AU2003251674B2 - Variants of the major allergen Phl p 1 from timothy grass - Google Patents
Variants of the major allergen Phl p 1 from timothy grass Download PDFInfo
- Publication number
- AU2003251674B2 AU2003251674B2 AU2003251674A AU2003251674A AU2003251674B2 AU 2003251674 B2 AU2003251674 B2 AU 2003251674B2 AU 2003251674 A AU2003251674 A AU 2003251674A AU 2003251674 A AU2003251674 A AU 2003251674A AU 2003251674 B2 AU2003251674 B2 AU 2003251674B2
- Authority
- AU
- Australia
- Prior art keywords
- allergen
- variant
- timothy grass
- rphl
- phl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/02—Nasal agents, e.g. decongestants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
- A61P11/06—Antiasthmatics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/14—Decongestants or antiallergics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Pulmonology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Ophthalmology & Optometry (AREA)
- Otolaryngology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02018157 | 2002-08-19 | ||
| EP02018157.4 | 2002-08-19 | ||
| PCT/EP2003/008471 WO2004022588A1 (de) | 2002-08-19 | 2003-07-31 | Varianten des majorallergens phl p 1 aus lieschgras |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2003251674A1 AU2003251674A1 (en) | 2004-03-29 |
| AU2003251674B2 true AU2003251674B2 (en) | 2009-09-03 |
Family
ID=31970262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2003251674A Ceased AU2003251674B2 (en) | 2002-08-19 | 2003-07-31 | Variants of the major allergen Phl p 1 from timothy grass |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US7731970B2 (https=) |
| EP (1) | EP1532169B1 (https=) |
| JP (2) | JP4668616B2 (https=) |
| CN (1) | CN100509844C (https=) |
| AU (1) | AU2003251674B2 (https=) |
| BR (1) | BRPI0313613B1 (https=) |
| CA (1) | CA2495825C (https=) |
| ES (1) | ES2400181T3 (https=) |
| PL (1) | PL212891B1 (https=) |
| PT (1) | PT1532169E (https=) |
| RU (1) | RU2323942C2 (https=) |
| WO (1) | WO2004022588A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2323942C2 (ru) * | 2002-08-19 | 2008-05-10 | Мерк Патент Гмбх | ВАРИАНТЫ ОСНОВНОГО АЛЛЕРГЕНА Phl p 1 ИЗ ТИМОФЕЕВКИ ЛУГОВОЙ |
| DE102004035337A1 (de) * | 2004-07-21 | 2006-03-16 | Merck Patent Gmbh | Varianten der Gruppe 1-Allergene aus Poaceae mit reduzierter Allergenität und erhaltener T-Zellreaktivität |
| MX2014009285A (es) * | 2012-02-07 | 2015-02-04 | Jolla Inst Allergy Immunolog | Alergenos del fleo de los prados y metodos y usos para la modulacion de respuesta inmune. |
| JP6102442B2 (ja) * | 2013-04-04 | 2017-03-29 | 日産自動車株式会社 | リチウムイオン二次電池 |
| CN113825518A (zh) | 2019-01-17 | 2021-12-21 | 莱蒂生物制药公司 | 纯化过敏原提取物的方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05509230A (ja) * | 1990-08-17 | 1993-12-22 | ザ・ユニバーシテイ・オブ・メルボルン | ライグラスの花粉のアレルゲン |
| IL115177A0 (en) * | 1994-09-16 | 1995-12-31 | Immunomedics Inc | Phosphorus-32 labeling of antibodies for cancer therapy |
| US6281332B1 (en) * | 1994-12-02 | 2001-08-28 | The Johns Hopkins University School Of Medicine | Hedgehog-derived polypeptides |
| CA2267106A1 (en) * | 1996-10-07 | 1998-07-16 | The Johns Hopkins University School Of Medicine | Novel hedgehog-derived polypeptides |
| GB2348808B (en) * | 1998-01-09 | 2003-03-19 | Circassia Ltd | Methods and compositions for desensitisation |
| DE19918682A1 (de) * | 1999-04-23 | 2000-10-26 | Merck Patent Gmbh | DNA-Sequenz und rekombinante Herstellung eines Graminaen-Allergens |
| US20070011783A1 (en) * | 1999-05-06 | 2007-01-11 | Jingdong Liu | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| US20100293669A2 (en) * | 1999-05-06 | 2010-11-18 | Jingdong Liu | Nucleic Acid Molecules and Other Molecules Associated with Plants and Uses Thereof for Plant Improvement |
| IT1318692B1 (it) | 2000-09-12 | 2003-08-27 | Consiglio Nazionale Ricerche | Varianti di proteine allergeniche di phleum pratense. |
| AU2002256276A1 (en) * | 2001-04-19 | 2002-11-05 | Penn State Research Foundation | Novel expansin polynucleotides, related polypeptides and methods of use |
| RU2323942C2 (ru) * | 2002-08-19 | 2008-05-10 | Мерк Патент Гмбх | ВАРИАНТЫ ОСНОВНОГО АЛЛЕРГЕНА Phl p 1 ИЗ ТИМОФЕЕВКИ ЛУГОВОЙ |
-
2003
- 2003-07-31 RU RU2005107782/13A patent/RU2323942C2/ru not_active IP Right Cessation
- 2003-07-31 ES ES03793667T patent/ES2400181T3/es not_active Expired - Lifetime
- 2003-07-31 PT PT37936671T patent/PT1532169E/pt unknown
- 2003-07-31 WO PCT/EP2003/008471 patent/WO2004022588A1/de not_active Ceased
- 2003-07-31 JP JP2004533298A patent/JP4668616B2/ja not_active Expired - Fee Related
- 2003-07-31 CN CNB038198231A patent/CN100509844C/zh not_active Expired - Fee Related
- 2003-07-31 CA CA2495825A patent/CA2495825C/en not_active Expired - Lifetime
- 2003-07-31 US US10/525,000 patent/US7731970B2/en not_active Expired - Fee Related
- 2003-07-31 AU AU2003251674A patent/AU2003251674B2/en not_active Ceased
- 2003-07-31 EP EP03793667A patent/EP1532169B1/de not_active Expired - Lifetime
- 2003-07-31 PL PL373069A patent/PL212891B1/pl unknown
- 2003-07-31 BR BRPI0313613A patent/BRPI0313613B1/pt not_active IP Right Cessation
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2010
- 2010-03-22 US US12/728,745 patent/US9453058B2/en not_active Expired - Fee Related
- 2010-11-08 JP JP2010249581A patent/JP5868587B2/ja not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| Focke, M. et al., The FASEB Journal (2001): 1-25 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2495825A1 (en) | 2004-03-18 |
| RU2323942C2 (ru) | 2008-05-10 |
| JP5868587B2 (ja) | 2016-02-24 |
| US9453058B2 (en) | 2016-09-27 |
| ES2400181T3 (es) | 2013-04-08 |
| JP4668616B2 (ja) | 2011-04-13 |
| PL373069A1 (en) | 2005-08-08 |
| PT1532169E (pt) | 2013-03-18 |
| CN100509844C (zh) | 2009-07-08 |
| US20060251682A1 (en) | 2006-11-09 |
| JP2006516382A (ja) | 2006-07-06 |
| CN1675240A (zh) | 2005-09-28 |
| BRPI0313613B1 (pt) | 2016-06-07 |
| WO2004022588A1 (de) | 2004-03-18 |
| RU2005107782A (ru) | 2005-11-20 |
| EP1532169A1 (de) | 2005-05-25 |
| CA2495825C (en) | 2015-03-17 |
| US20120263741A1 (en) | 2012-10-18 |
| AU2003251674A1 (en) | 2004-03-29 |
| JP2011097940A (ja) | 2011-05-19 |
| EP1532169B1 (de) | 2012-12-19 |
| PL212891B1 (pl) | 2012-12-31 |
| US7731970B2 (en) | 2010-06-08 |
| BR0313613A (pt) | 2005-06-21 |
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