ATE497019T1 - Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen - Google Patents
Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzenInfo
- Publication number
- ATE497019T1 ATE497019T1 AT06117641T AT06117641T ATE497019T1 AT E497019 T1 ATE497019 T1 AT E497019T1 AT 06117641 T AT06117641 T AT 06117641T AT 06117641 T AT06117641 T AT 06117641T AT E497019 T1 ATE497019 T1 AT E497019T1
- Authority
- AT
- Austria
- Prior art keywords
- targets
- probes
- discrimination
- oligonucleotide probes
- oligonucleotide
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 3
- 238000009396 hybridization Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 3
- 125000003729 nucleotide group Chemical group 0.000 abstract 3
- 239000002751 oligonucleotide probe Substances 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 238000010804 cDNA synthesis Methods 0.000 abstract 1
- 239000002299 complementary DNA Substances 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 238000002844 melting Methods 0.000 abstract 1
- 230000008018 melting Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 102000054765 polymorphisms of proteins Human genes 0.000 abstract 1
- 210000003296 saliva Anatomy 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Monitoring And Testing Of Transmission In General (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0007622A GB0007622D0 (en) | 2000-03-29 | 2000-03-29 | Hybridisation beacon and method of sequence detection |
| GB0026749A GB0026749D0 (en) | 2000-11-02 | 2000-11-02 | Hybridisation beacon and rapid method of sequence detection and discrimination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE497019T1 true ATE497019T1 (de) | 2011-02-15 |
Family
ID=26243981
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01915549T ATE334228T1 (de) | 2000-03-29 | 2001-03-28 | Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen |
| AT06117641T ATE497019T1 (de) | 2000-03-29 | 2001-03-28 | Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01915549T ATE334228T1 (de) | 2000-03-29 | 2001-03-28 | Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US7348141B2 (de) |
| EP (2) | EP1278889B1 (de) |
| JP (1) | JP5112592B2 (de) |
| AT (2) | ATE334228T1 (de) |
| AU (2) | AU4263401A (de) |
| CA (1) | CA2404174C (de) |
| DE (2) | DE60143974D1 (de) |
| DK (2) | DK1278889T3 (de) |
| ES (1) | ES2269365T3 (de) |
| NZ (1) | NZ521593A (de) |
| PT (2) | PT1278889E (de) |
| WO (1) | WO2001073118A2 (de) |
Families Citing this family (118)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7998673B2 (en) * | 2000-03-29 | 2011-08-16 | Lgc Limited | Hybridisation beacon and method of rapid sequence detection and discrimination |
| CA2417986C (en) | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
| WO2003018837A2 (de) * | 2001-08-24 | 2003-03-06 | Adnagen Ag | Verfahren und diagnosekit zur molekularen diagnostik pharmakologisch relevanter gene |
| AU2003226829A1 (en) * | 2002-02-11 | 2003-09-04 | Applied Research Systems Ars Holding N.V. | Nucleotide detection by fluorescence resonance energy transfer and methods thereof |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| ATE400663T1 (de) | 2002-02-21 | 2008-07-15 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| EP1721001A4 (de) * | 2004-02-04 | 2007-08-29 | Park Hee Kyung | Qualitätskontrollsonden umfassender mikroarray und verfahren zur herstellung davon |
| AU2006251937A1 (en) * | 2005-05-27 | 2006-11-30 | John Wayne Cancer Institute | Use of free circulating DNA for diagnosis, prognosis, and treatment of cancer |
| GB0514889D0 (en) * | 2005-07-20 | 2005-08-24 | Lgc Ltd | Oligonucleotides |
| ES2420831T3 (es) | 2005-07-25 | 2013-08-27 | Alere San Diego, Inc. | Procedimientos para multiplexar la ampliación de la recombinasa polimerasa |
| WO2007076129A2 (en) | 2005-12-23 | 2007-07-05 | Nanostring Technologies, Inc. | Nanoreporters and methods of manufacturing and use thereof |
| US8133984B2 (en) | 2006-03-16 | 2012-03-13 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| EP2426221B1 (de) | 2006-05-04 | 2016-06-22 | Alere San Diego, Inc. | Rekombinase-Polymerase-Amplifikation |
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| WO2008014485A2 (en) | 2006-07-28 | 2008-01-31 | California Institute Of Technology | Multiplex q-pcr arrays |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| KR101110396B1 (ko) * | 2006-08-08 | 2012-07-04 | 아크레이 가부시키가이샤 | 변이의 검출 방법 및 그것에 이용하는 키트 |
| EP2048247B1 (de) * | 2006-08-09 | 2014-06-18 | ARKRAY, Inc. | Herstellungsverfahren für pcr-amplifikationsprodukt und seine verwendung |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| KR101119417B1 (ko) * | 2006-11-30 | 2012-04-12 | 아크레이 가부시키가이샤 | 비만 유전자 증폭용 프라이머 세트, 그것을 포함하는 비만 유전자 증폭용 시약 및 그 용도 |
| WO2008066161A1 (en) * | 2006-11-30 | 2008-06-05 | Arkray, Inc. | Primer set for amplification of nat2 gene, reagent for amplification of nat2 gene comprising the same, and use of the same |
| EP2055789A4 (de) * | 2006-11-30 | 2009-12-23 | Arkray Inc | Primer-set zur verstärkung des cyp2c19-gens, reagens zur verstärkung des cyp2c19-gens damit und anwendung davon |
| US9938641B2 (en) * | 2006-12-18 | 2018-04-10 | Fluidigm Corporation | Selection of aptamers based on geometry |
| JP4942508B2 (ja) * | 2007-02-20 | 2012-05-30 | アークレイ株式会社 | ａｂｌ遺伝子変異の検出用プローブおよびその用途 |
| JP4410269B2 (ja) | 2007-03-28 | 2010-02-03 | 株式会社東芝 | Ｎ−アセチルトランスフェラーゼ２（ｎａｔ２）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法 |
| JP2009148245A (ja) * | 2007-06-12 | 2009-07-09 | Toyobo Co Ltd | 核酸の迅速な検出方法 |
| WO2008156591A1 (en) * | 2007-06-15 | 2008-12-24 | The Feinstein Institute Medical Research | Prediction of schizophrenia risk using homozygous genetic markers |
| ATE516371T1 (de) | 2007-10-22 | 2011-07-15 | Lgc Ltd | Oligonukleotide und deren verwendungen |
| WO2009081967A1 (ja) * | 2007-12-26 | 2009-07-02 | Arkray, Inc. | 標的核酸配列の増幅方法およびそれに用いるプローブ |
| WO2010018245A1 (es) * | 2008-08-12 | 2010-02-18 | Biotools Biotechnological & Medical Laboratories, S.A. | Método para la detección y/o cuantificación de un ácido nucleico substrato |
| EP2394152B1 (de) | 2009-02-03 | 2019-05-01 | ANDE Corporation | Nukleinsäureaufreinigung |
| US9469867B2 (en) | 2009-05-20 | 2016-10-18 | Alere San Diego, Inc. | DNA glycosylase/lyase and AP endonuclease substrates |
| US8999675B2 (en) | 2009-08-31 | 2015-04-07 | Gen-Probe Incorporated | Dengue virus assay |
| WO2011027966A2 (en) | 2009-09-03 | 2011-03-10 | Seegene, Inc. | Td probe and its uses |
| PL389135A1 (pl) * | 2009-09-28 | 2011-04-11 | Michał Lower | Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej |
| US20110136105A1 (en) * | 2009-12-05 | 2011-06-09 | Evogen, Inc. | Methods of quantifying nucleic acids |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| GB201010237D0 (en) | 2010-06-18 | 2010-07-21 | Lgc Ltd | Methods and apparatuses |
| MX352460B (es) | 2011-01-11 | 2017-11-24 | Seegene Inc | Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto. |
| AU2012278784B2 (en) * | 2011-07-06 | 2015-12-24 | The Johns Hopkins University | Semi-digital ligation assay |
| CN108866169A (zh) | 2011-07-06 | 2018-11-23 | 探索诊断投资公司 | 病毒和细菌病原体的直接扩增和检测 |
| CA2851923A1 (en) * | 2011-10-14 | 2013-04-18 | Accugenomics, Inc. | Nucleic acid amplification and use thereof |
| US8981318B1 (en) | 2011-12-30 | 2015-03-17 | Gene Capture, Inc. | Multi-dimensional scanner for nano-second time scale signal detection |
| EP4361608A3 (de) | 2012-02-03 | 2024-07-24 | California Institute of Technology | Signalkodierung und -dekodierung in multiplexierten biochemischen assays |
| GB2513024B (en) | 2012-02-27 | 2016-08-31 | Cellular Res Inc | A clonal amplification method |
| KR101794981B1 (ko) | 2012-03-05 | 2017-11-09 | 주식회사 씨젠 | Ｐｔｏ 절단 및 연장 어세이에 의한 타겟 핵산서열에서의 뉴클레오타이드 변이 검출 |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| EP3901243A1 (de) | 2012-08-03 | 2021-10-27 | California Institute of Technology | Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen |
| ES2662825T3 (es) | 2013-02-25 | 2018-04-09 | Seegene, Inc. | Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana |
| JP2016508733A (ja) | 2013-03-13 | 2016-03-24 | シージーン アイエヌシー | メルティングピーク分析を利用したターゲット核酸配列の定量 |
| GB201310823D0 (en) * | 2013-06-18 | 2013-07-31 | Lgc Ltd | Fluorophore-based oligonucleotide probes with a universal element |
| GB2546833B (en) | 2013-08-28 | 2018-04-18 | Cellular Res Inc | Microwell for single cell analysis comprising single cell and single bead oligonucleotide capture labels |
| WO2015057008A2 (en) | 2013-10-18 | 2015-04-23 | Seegene, Inc. | Detection of target nucleic acid sequence on solid phase by pto cleavage and extension using hcto assay |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| WO2016065207A1 (en) * | 2014-10-23 | 2016-04-28 | Ricardo Mancebo | Reagents and methods for isothermal chain reaction |
| US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
| EP3347465B1 (de) * | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Verfahren und zusammensetzung zur standardisierung von nukleinsäure bibliothek |
| EP3378000B1 (de) | 2015-11-20 | 2021-09-01 | Seegene, Inc. | Verfahren zur kalibrierung eines datensatzes eines zielanalyts |
| KR102300738B1 (ko) | 2016-02-05 | 2021-09-10 | 주식회사 씨젠 | 타겟 분석물질에 대한 데이터 세트의 노이즈 수준 감축 방법 |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| CN109601008B (zh) | 2016-04-01 | 2023-02-28 | 克罗玛科德公司 | 用于工程化信号产生的竞争性探针 |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| WO2017218777A1 (en) | 2016-06-17 | 2017-12-21 | California Institute Of Technology | Nucleic acid reactions and related methods and compositions |
| KR102301305B1 (ko) | 2016-06-30 | 2021-09-13 | 주식회사 씨젠 | 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기 |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| WO2018066950A1 (en) | 2016-10-06 | 2018-04-12 | Seegene, Inc. . | Methods for preparing oligonucleotides for detecting target nucleic acid molecules in samples |
| JP6938641B2 (ja) | 2016-12-29 | 2021-09-22 | シージーン アイエヌシー | プライマーダイマー形成を減少させ、増幅効率を増加させる方法 |
| WO2018144240A1 (en) | 2017-02-01 | 2018-08-09 | Cellular Research, Inc. | Selective amplification using blocking oligonucleotides |
| KR102408564B1 (ko) | 2017-03-28 | 2022-06-14 | 주식회사 씨젠 | 타겟 핵산 서열의 존재를 결정하기 위한 분석 시그널 |
| AU2018281745B2 (en) | 2017-06-05 | 2022-05-19 | Becton, Dickinson And Company | Sample indexing for single cells |
| WO2019045532A2 (en) | 2017-08-31 | 2019-03-07 | Seegene, Inc. | EVALUATION OF COMPONENT PERFORMANCE USING A PAIR OF DIMER FORMER PRIMERS |
| AU2018339207A1 (en) | 2017-09-28 | 2020-04-16 | Seegene, Inc. . | Method and device for analyzing target analyte in sample |
| US11401546B2 (en) | 2017-09-29 | 2022-08-02 | Seegene, Inc. | Detection of target nucleic acid sequences by PTO cleavage and extension-dependent extension assay |
| CN112166199B (zh) | 2018-04-02 | 2025-04-29 | 埃努梅拉分子股份有限公司 | 用于对核酸分子进行计数的方法、系统和组合物 |
| US12454720B2 (en) | 2018-04-17 | 2025-10-28 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| EP3781708B1 (de) | 2018-04-20 | 2025-01-15 | Seegene, Inc. | Verfahren zur detektion einer vielzahl von zielnukleinsäuresequenzen in einer probe |
| EP3788170B1 (de) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molekulare barcodierung auf gegenüberliegenden transkriptenden |
| KR101919896B1 (ko) * | 2018-05-03 | 2018-11-19 | 주식회사 유디피아 | 단일 형광 채널 유전자증폭기기를 이용한 다중 핵산 검출 방법 |
| ES2945191T3 (es) | 2018-05-03 | 2023-06-29 | Becton Dickinson Co | Análisis de muestras multiómicas de alto rendimiento |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| EP4471156A3 (de) | 2018-10-01 | 2025-02-26 | Becton, Dickinson and Company | Bestimmung von 5'-transkriptsequenzen |
| WO2020097315A1 (en) | 2018-11-08 | 2020-05-14 | Cellular Research, Inc. | Whole transcriptome analysis of single cells using random priming |
| JP7035972B2 (ja) * | 2018-11-09 | 2022-03-15 | 横河電機株式会社 | 核酸配列計測用デバイス |
| CN113195717A (zh) | 2018-12-13 | 2021-07-30 | 贝克顿迪金森公司 | 单细胞全转录组分析中的选择性延伸 |
| WO2020154247A1 (en) | 2019-01-23 | 2020-07-30 | Cellular Research, Inc. | Oligonucleotides associated with antibodies |
| EP3924506A1 (de) | 2019-02-14 | 2021-12-22 | Becton Dickinson and Company | Hybride gezielte und gesamttranskriptomamplifikation |
| EP3937780A4 (de) | 2019-03-14 | 2022-12-07 | InSilixa, Inc. | Verfahren und systeme zur zeitgesteuerten fluoreszenzbasierten detektion |
| EP3947718A4 (de) | 2019-04-02 | 2022-12-21 | Enumera Molecular, Inc. | Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen |
| CN114051534B (zh) | 2019-07-22 | 2025-02-21 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| EP4407041A3 (de) | 2019-11-08 | 2024-09-25 | Becton Dickinson and Company | Verwendung von random-rpiming zur gewinnung von v(d)j-informationen in voller länge für immunrepertoiresequenzierung |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| EP4471155A3 (de) | 2020-01-29 | 2024-12-18 | Becton, Dickinson and Company | Strichcodierte bohrlöcher zur räumlichen abbildung einzelner zellen durch sequenzierung |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| US11149320B1 (en) | 2020-03-31 | 2021-10-19 | Diasorin S.P.A. | Assays for the detection of SARS-CoV-2 |
| CN115605614A (zh) | 2020-05-14 | 2023-01-13 | 贝克顿迪金森公司（Us） | 用于免疫组库谱分析的引物 |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| KR20240058112A (ko) | 2021-09-17 | 2024-05-03 | 주식회사 씨젠 | 합성 비자연 염기를 포함하는 태그 올리고뉴클레오타이드를 이용한 타겟 핵산 서열의 검출 |
| KR20240056605A (ko) | 2021-09-30 | 2024-04-30 | 주식회사 씨젠 | 분자진단 시스템의 샘플 처리 및 분석 방법 |
| EP4421814A4 (de) | 2021-10-21 | 2025-11-12 | Seegene Inc | Vorrichtung und verfahren zum lesen von positiv/negativen informationen über eine zieltestsubstanz in einer probe |
| US20250043328A1 (en) | 2021-12-10 | 2025-02-06 | Nitto Boseki Co., Ltd. | Agent for raising melting temperature of double-stranded nucleic acid, and uses therefor |
| WO2023214843A1 (ko) | 2022-05-06 | 2023-11-09 | 주식회사 씨젠 | 핵산 검출 카트리지 |
| KR20250022719A (ko) | 2022-06-15 | 2025-02-17 | 주식회사 씨젠 | 기술 문서를 생성하는 방법 및 장치 |
| EP4553489A1 (de) | 2022-07-05 | 2025-05-14 | Seegene, Inc. | Verfahren zur gewinnung eines annähernden signals für jeden von mehreren zielanalyten und computervorrichtung zur durchführung davon |
| US20250336479A1 (en) | 2022-07-29 | 2025-10-30 | Seegene, Inc | Apparatus and method for providing interface for setting threshold value of parameter |
| KR20250086619A (ko) | 2022-10-14 | 2025-06-13 | 주식회사 씨젠 | 상이한 tm 값을 가지는 2개의 프로브를 이용하여 샘플 내 타겟 핵산을 검출하는 방법 |
| WO2024085731A1 (ko) | 2022-10-20 | 2024-04-25 | 주식회사 씨젠 | 타겟 핵산 분자 검출용 시약 개발에 필요한 복수 개의 세부 성능 실험 가이딩 및 실험 결과 기록서 정리 방법 |
| EP4621068A1 (de) | 2022-11-16 | 2025-09-24 | Seegene, Inc. | Nachweis von zielnukleinsäuren durch quenching-pto-spaltungs- und verlängerungs-(q-ptoce)-assay |
| WO2024128878A1 (ko) | 2022-12-15 | 2024-06-20 | 주식회사 씨젠 | 형광 데이터의 분석 알고리즘에 대한 성능 비교 결과를 디스플레이하는 장치 및 방법 |
| WO2025141105A1 (en) | 2023-12-27 | 2025-07-03 | bioMérieux | Specific probes for the detection of nucleic acids, methods and uses |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656493A (en) * | 1985-03-28 | 1997-08-12 | The Perkin-Elmer Corporation | System for automated performance of the polymerase chain reaction |
| US4889818A (en) * | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| EP0533906A4 (en) * | 1991-04-11 | 1994-09-28 | Baxter Diagnostics Inc | Detection of dna/rna by fluorescence polarization |
| WO1994002634A1 (en) * | 1992-07-24 | 1994-02-03 | University Of South Australia | Amplification and detection process |
| US5746231A (en) * | 1993-01-11 | 1998-05-05 | Craig Lesser | Tobacco smoke filter for removing toxic compounds |
| US5501238A (en) * | 1993-01-11 | 1996-03-26 | Von Borstel; Reid W. | Cigarette filter containing a humectant |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5597696A (en) * | 1994-07-18 | 1997-01-28 | Becton Dickinson And Company | Covalent cyanine dye oligonucleotide conjugates |
| US5817461A (en) * | 1996-01-03 | 1998-10-06 | Hamilton Civic Hospitals Research Development Inc. | Methods and compositions for diagnosis of hyperhomocysteinemia |
| SE506700C2 (sv) * | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
| DE69735445T2 (de) * | 1996-12-10 | 2006-08-10 | Sequenom, Inc., San Diego | Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung |
| US6451530B1 (en) * | 1996-12-13 | 2002-09-17 | The United States Of America As Represented By The Department Of Health And Human Services | Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization |
| US5846603A (en) * | 1997-07-28 | 1998-12-08 | Superior Fibers, Inc. | Uniformly tacky filter media |
| AU9220498A (en) * | 1997-09-04 | 1999-03-22 | Bayer Corporation | Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof |
| AU1366299A (en) * | 1997-10-27 | 1999-05-17 | Boston Probes, Inc. | Methods, kits and compositions pertaining to pna molecular beacons |
| US5952202A (en) * | 1998-03-26 | 1999-09-14 | The Perkin Elmer Corporation | Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification |
| NZ528967A (en) | 1999-06-22 | 2005-03-24 | Invitrogen Corp | Improved primers and methods for the amplification and discrimination of nucleic acids |
| SE9902565D0 (sv) * | 1999-07-05 | 1999-07-05 | Jonas Karlsson | Probe for analysis of nucleic acids |
| FR2805348B1 (fr) * | 2000-02-23 | 2002-07-12 | Commissariat Energie Atomique | Analyse de cibles biologiques utilisant une biopuce comportant un marqueur fluorescent |
| CA2417986C (en) * | 2000-08-11 | 2013-11-26 | University Of Utah Research Foundation | Single-labeled oligonucleotide probes |
-
2001
- 2001-03-28 US US10/239,913 patent/US7348141B2/en not_active Expired - Lifetime
- 2001-03-28 JP JP2001570832A patent/JP5112592B2/ja not_active Expired - Fee Related
- 2001-03-28 EP EP01915549A patent/EP1278889B1/de not_active Expired - Lifetime
- 2001-03-28 EP EP06117641A patent/EP1715063B1/de not_active Expired - Lifetime
- 2001-03-28 AT AT01915549T patent/ATE334228T1/de active
- 2001-03-28 PT PT01915549T patent/PT1278889E/pt unknown
- 2001-03-28 PT PT06117641T patent/PT1715063E/pt unknown
- 2001-03-28 DE DE60143974T patent/DE60143974D1/de not_active Expired - Lifetime
- 2001-03-28 AU AU4263401A patent/AU4263401A/xx active Pending
- 2001-03-28 AT AT06117641T patent/ATE497019T1/de active
- 2001-03-28 DK DK01915549T patent/DK1278889T3/da active
- 2001-03-28 CA CA2404174A patent/CA2404174C/en not_active Expired - Lifetime
- 2001-03-28 DK DK06117641.8T patent/DK1715063T3/da active
- 2001-03-28 AU AU2001242634A patent/AU2001242634B2/en not_active Expired
- 2001-03-28 NZ NZ521593A patent/NZ521593A/en not_active IP Right Cessation
- 2001-03-28 WO PCT/GB2001/001430 patent/WO2001073118A2/en not_active Ceased
- 2001-03-28 ES ES01915549T patent/ES2269365T3/es not_active Expired - Lifetime
- 2001-03-28 DE DE60121750T patent/DE60121750T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP5112592B2 (ja) | 2013-01-09 |
| US7348141B2 (en) | 2008-03-25 |
| AU4263401A (en) | 2001-10-08 |
| EP1278889B1 (de) | 2006-07-26 |
| DK1278889T3 (da) | 2006-11-27 |
| EP1715063B1 (de) | 2011-01-26 |
| NZ521593A (en) | 2004-11-26 |
| CA2404174A1 (en) | 2001-10-04 |
| DE60121750T2 (de) | 2007-08-16 |
| DE60121750D1 (de) | 2006-09-07 |
| PT1715063E (pt) | 2011-04-28 |
| CA2404174C (en) | 2010-05-18 |
| EP1278889A2 (de) | 2003-01-29 |
| US20040091864A1 (en) | 2004-05-13 |
| ATE334228T1 (de) | 2006-08-15 |
| PT1278889E (pt) | 2006-12-29 |
| WO2001073118A2 (en) | 2001-10-04 |
| ES2269365T3 (es) | 2007-04-01 |
| DE60143974D1 (de) | 2011-03-10 |
| DK1715063T3 (da) | 2011-05-16 |
| JP2003528626A (ja) | 2003-09-30 |
| EP1715063A2 (de) | 2006-10-25 |
| WO2001073118A3 (en) | 2002-09-12 |
| EP1715063A3 (de) | 2007-05-02 |
| AU2001242634B2 (en) | 2006-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE497019T1 (de) | Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen | |
| Whitcombe et al. | Advances in approaches to DNA-based diagnostics | |
| US6287781B1 (en) | Method for detection of target nucleic acids using PCR | |
| US10570462B2 (en) | Kits and reaction mixtures for analyzing single-stranded nucleic acid sequences | |
| US20020098484A1 (en) | Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion | |
| WO2003066803A2 (en) | Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection | |
| JP2005525787A (ja) | プローブとの相互作用による遺伝子ハプロタイプの検出方法 | |
| CA2704487A1 (en) | One-step target detection assay | |
| KR20040024556A (ko) | 검출 시스템 | |
| Nurmi et al. | High-throughput genetic analysis using time-resolved fluorometry and closed-tube detection | |
| Rickert et al. | Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan | |
| CN101705292A (zh) | 基于核酸外切酶ⅲ/ⅰ保护分析检测单核苷酸多态性的荧光生物传感方法 | |
| JP4190562B2 (ja) | 遺伝子配列検査法 | |
| CN101285095B (zh) | 一种检测脱氧核糖核酸的单核苷酸多态性的方法 | |
| Louis et al. | Rapid combined genotyping of factor V, prothrombin and methylenetetrahydrofolate reductase single nucleotide polymorphisms using minor groove binding DNA oligonucleotides (MGB probes) and real-time polymerase chain reaction. | |
| CN107400722B (zh) | 一种检测人基因组的竞争性实时荧光pcr snp探针 | |
| CA2896616C (en) | Improved calibration of high resolution melting | |
| JP3974557B2 (ja) | 変異解析方法及びそのためのシステム | |
| Lehmusvuori et al. | Closed-tube human leukocyte antigen DQA1∗ 05 genotyping assay based on switchable lanthanide luminescence probes | |
| US20210180115A1 (en) | Multiple analysis method for amplicon by using fluorescence-based multiple melting analysis | |
| JP4310675B2 (ja) | 塩基多型の同定方法 | |
| EP1354063A2 (de) | Detektion spezifischer nukleotidsequenzen | |
| US20030124581A1 (en) | Newborn screening for hemoglobinopathy by DNA microarray analysis | |
| JP2003135098A (ja) | 遺伝子検査方法 | |
| US20040101868A1 (en) | Analysis method for hemochromatosis mutation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1715063 Country of ref document: EP |