ATE497019T1 - Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen - Google Patents

Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen

Info

Publication number
ATE497019T1
ATE497019T1 AT06117641T AT06117641T ATE497019T1 AT E497019 T1 ATE497019 T1 AT E497019T1 AT 06117641 T AT06117641 T AT 06117641T AT 06117641 T AT06117641 T AT 06117641T AT E497019 T1 ATE497019 T1 AT E497019T1
Authority
AT
Austria
Prior art keywords
targets
probes
discrimination
oligonucleotide probes
oligonucleotide
Prior art date
Application number
AT06117641T
Other languages
English (en)
Inventor
David John French
David Gordon Mcdowell
Tom Brown
Original Assignee
Lgc Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0007622A external-priority patent/GB0007622D0/en
Priority claimed from GB0026749A external-priority patent/GB0026749D0/en
Application filed by Lgc Ltd filed Critical Lgc Ltd
Application granted granted Critical
Publication of ATE497019T1 publication Critical patent/ATE497019T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6818Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Monitoring And Testing Of Transmission In General (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT06117641T 2000-03-29 2001-03-28 Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen ATE497019T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0007622A GB0007622D0 (en) 2000-03-29 2000-03-29 Hybridisation beacon and method of sequence detection
GB0026749A GB0026749D0 (en) 2000-11-02 2000-11-02 Hybridisation beacon and rapid method of sequence detection and discrimination

Publications (1)

Publication Number Publication Date
ATE497019T1 true ATE497019T1 (de) 2011-02-15

Family

ID=26243981

Family Applications (2)

Application Number Title Priority Date Filing Date
AT01915549T ATE334228T1 (de) 2000-03-29 2001-03-28 Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen
AT06117641T ATE497019T1 (de) 2000-03-29 2001-03-28 Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT01915549T ATE334228T1 (de) 2000-03-29 2001-03-28 Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen

Country Status (12)

Country Link
US (1) US7348141B2 (de)
EP (2) EP1715063B1 (de)
JP (1) JP5112592B2 (de)
AT (2) ATE334228T1 (de)
AU (2) AU2001242634B2 (de)
CA (1) CA2404174C (de)
DE (2) DE60143974D1 (de)
DK (2) DK1278889T3 (de)
ES (1) ES2269365T3 (de)
NZ (1) NZ521593A (de)
PT (2) PT1278889E (de)
WO (1) WO2001073118A2 (de)

Families Citing this family (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7998673B2 (en) * 2000-03-29 2011-08-16 Lgc Limited Hybridisation beacon and method of rapid sequence detection and discrimination
JP5213297B2 (ja) * 2000-08-11 2013-06-19 ユニバーシティ オブ ユタ リサーチ ファウンデーション 単一ラベルオリゴヌクレオチド・プローブ
WO2003018837A2 (de) * 2001-08-24 2003-03-06 Adnagen Ag Verfahren und diagnosekit zur molekularen diagnostik pharmakologisch relevanter gene
BR0303224A (pt) * 2002-02-11 2004-07-06 Applied Research Systems Detecção de nucleotìdeos por transferência de energia de ressonância fluorescente e métodos para a mesma
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
EP1992706B1 (de) 2002-02-21 2014-11-19 Alere San Diego, Inc. Rekombinase-Polymerase-Verstärkung
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
WO2005075682A1 (en) * 2004-02-04 2005-08-18 Hee-Kyung Park Microarray comprising qc probes and method for fabricating the same
EP1888786A4 (de) * 2005-05-27 2009-12-30 Wayne John Cancer Inst Verwendung freier zirkulierender dna zur diagnose, prognose und behandlung von krebs
GB0514889D0 (en) * 2005-07-20 2005-08-24 Lgc Ltd Oligonucleotides
US8062850B2 (en) 2005-07-25 2011-11-22 Alere San Diego, Inc. Methods for multiplexing recombinase polymerase amplification
AU2006330830B2 (en) 2005-12-23 2013-01-10 Bruker Spatial Biology, Inc. Nanoreporters and methods of manufacturing and use thereof
US8133984B2 (en) 2006-03-16 2012-03-13 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
CA2650993C (en) 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
WO2008014485A2 (en) 2006-07-28 2008-01-31 California Institute Of Technology Multiplex q-pcr arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
JPWO2008018305A1 (ja) * 2006-08-08 2009-12-24 アークレイ株式会社 変異の検出方法およびそれに用いるキット
CN101443457B (zh) * 2006-08-09 2015-05-27 爱科来株式会社 利用pcr的扩增产物的制造方法及其用途
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
EP2055776A4 (de) * 2006-11-30 2009-11-25 Arkray Inc Primer-set zur verstärkung des adipositas-gens, reagens zur verstärkung des adipositas-gens damit und anwendung davon
JPWO2008066161A1 (ja) * 2006-11-30 2010-03-11 アークレイ株式会社 Nat2遺伝子増幅用プライマーセット、それを含むnat2遺伝子増幅用試薬およびその用途
WO2008066162A1 (en) * 2006-11-30 2008-06-05 Arkray, Inc. Primer set for amplification of cyp2c19 gene, reagent for amplification of cyp2c19 gene comprising the same, and use of the same
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
JP4942508B2 (ja) * 2007-02-20 2012-05-30 アークレイ株式会社 abl遺伝子変異の検出用プローブおよびその用途
JP4410269B2 (ja) 2007-03-28 2010-02-03 株式会社東芝 N−アセチルトランスフェラーゼ2(nat2)の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
JP2009148245A (ja) * 2007-06-12 2009-07-09 Toyobo Co Ltd 核酸の迅速な検出方法
US20100285455A1 (en) * 2007-06-15 2010-11-11 The Feinstein Institute Medical Research Prediction of schizophrenia risk using homozygous genetic markers
WO2009053679A1 (en) * 2007-10-22 2009-04-30 Lgc Limited Oligonucleotides and uses thereof
KR20090127893A (ko) * 2007-12-26 2009-12-14 아크레이 가부시키가이샤 표적 핵산 서열의 증폭 방법 및 그것에 이용하는 프로브
WO2010018245A1 (es) * 2008-08-12 2010-02-18 Biotools Biotechnological & Medical Laboratories, S.A. Método para la detección y/o cuantificación de un ácido nucleico substrato
US9012208B2 (en) 2009-02-03 2015-04-21 Netbio, Inc. Nucleic acid purification
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP2473624B1 (de) 2009-08-31 2019-05-01 Gen-Probe Incorporated Test für dengue-virus
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
PL389135A1 (pl) * 2009-09-28 2011-04-11 Michał Lower Sposób i sonda DNA do otrzymywania endonukleaz restrukcyjnych, zwłaszcza o pożądanej specyficzności sekwencyjnej
US20110136105A1 (en) * 2009-12-05 2011-06-09 Evogen, Inc. Methods of quantifying nucleic acids
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
GB201010237D0 (en) 2010-06-18 2010-07-21 Lgc Ltd Methods and apparatuses
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
US20140155275A1 (en) * 2011-07-06 2014-06-05 The Johns Hopkins University Semi-digital ligation assay
EP4083230B1 (de) * 2011-07-06 2025-12-03 Quest Diagnostics Investments Incorporated Direkte amplifikation und nachweis von viralen und bakteriellen krankheitserregern
CA2851923A1 (en) * 2011-10-14 2013-04-18 Accugenomics, Inc. Nucleic acid amplification and use thereof
US8981318B1 (en) 2011-12-30 2015-03-17 Gene Capture, Inc. Multi-dimensional scanner for nano-second time scale signal detection
EP2809813B1 (de) 2012-02-03 2018-08-08 California Institute of Technology Signalcodierung und -decodierung bei biochemischen multiplextests
HK1206069A1 (en) 2012-02-27 2015-12-31 Cellular Research, Inc. Compositions and kits for molecular counting
CA2864523C (en) 2012-03-05 2019-02-05 Seegene, Inc. Detection of nucleotide variation on target nucleic acid sequence by pto cleavage and extension assay
AU2013202808B2 (en) 2012-07-31 2014-11-13 Gen-Probe Incorporated System and method for performing multiplex thermal melt analysis
CN104662172B (zh) 2012-08-03 2018-07-06 加州理工学院 Pcr中具有减少的硬件和要求的多重化和定量
ES2662825T3 (es) 2013-02-25 2018-04-09 Seegene, Inc. Detección de variación de nucleótido en una secuencia de ácidos nucleicos diana
EP2971115B1 (de) 2013-03-13 2022-07-27 Seegene, Inc. Quantifizierung von zielnukleinsäuren unter verwendung von schmelzspitzenwertanalyse
GB201310823D0 (en) * 2013-06-18 2013-07-31 Lgc Ltd Fluorophore-based oligonucleotide probes with a universal element
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
US11447814B2 (en) 2013-10-18 2022-09-20 Seegene, Inc. Detection of target nucleic acid sequence on solid phase by PTO cleavage and extension using HCTO assay
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP4098753A1 (de) * 2014-10-23 2022-12-07 Ricardo Mancebo Reagenzien und verfahren für eine isothermische kettenreaktion
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
EP3277843A2 (de) 2015-03-30 2018-02-07 Cellular Research, Inc. Verfahren und zusammensetzungen für kombinatorische barcodierung
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
ES2745694T3 (es) * 2015-09-11 2020-03-03 Cellular Res Inc Métodos y composiciones para normalización de biblioteca de ácido nucleico
KR102270771B1 (ko) 2015-11-20 2021-06-29 주식회사 씨젠 표적 분석물질에 대한 데이터 세트의 보정 방법
EP3411819B1 (de) 2016-02-05 2025-08-20 Seegene, Inc. Verfahren zur reduzierung des rauschpegels eines datensatzes für einen zielanalyten
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN109601008B (zh) 2016-04-01 2023-02-28 克罗玛科德公司 用于工程化信号产生的竞争性探针
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109642252A (zh) 2016-06-17 2019-04-16 加州理工学院 核酸反应及相关方法和组合物
KR102301305B1 (ko) 2016-06-30 2021-09-13 주식회사 씨젠 핵산 증폭용 튜브 및 핵산 증폭용 반응 용기
SG11201901733PA (en) 2016-09-26 2019-04-29 Cellular Res Inc Measurement of protein expression using reagents with barcoded oligonucleotide sequences
US11222713B2 (en) 2016-10-06 2022-01-11 Seegene, Inc. Methods for preparing oligonucleotides for detecting target nucleic acid molecules in samples
KR102535892B1 (ko) 2016-12-29 2023-05-26 주식회사 씨젠 프라이머 다이머 형성을 감소시키고 증폭 효율을 증가시키는 방법
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
WO2018182281A1 (en) 2017-03-28 2018-10-04 Seegene, Inc. . Analytical signal for determination of the presence of a target nucleic acid sequence
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
US11591645B2 (en) 2017-08-31 2023-02-28 Seegene, Inc. Evaluation of performance of components using a pair of dimer-forming primers
JP7012151B2 (ja) 2017-09-28 2022-01-27 シージーン アイエヌシー 試料内ターゲット分析物を分析する方法及び装置
EP3688188A4 (de) 2017-09-29 2021-06-16 Seegene, Inc. Detektion von zielnukleinsäuresequenzen durch pto-spaltung und erweiterungsabhängiger nicht-hybridisierungstest
EP4647508A2 (de) 2018-04-02 2025-11-12 Enumera Molecular, Inc. Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen
WO2019204357A1 (en) 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
ES3010286T3 (en) 2018-04-20 2025-04-02 Seegene Inc Method for detecting a plurality of target nucleic acid sequences in sample
KR101919896B1 (ko) * 2018-05-03 2018-11-19 주식회사 유디피아 단일 형광 채널 유전자증폭기기를 이용한 다중 핵산 검출 방법
JP7407128B2 (ja) 2018-05-03 2023-12-28 ベクトン・ディキンソン・アンド・カンパニー ハイスループットマルチオミクスサンプル解析
CN118910215A (zh) 2018-05-03 2024-11-08 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
EP3861134B1 (de) 2018-10-01 2024-09-04 Becton, Dickinson and Company Bestimmung von 5'-transkriptsequenzen
CN112969789A (zh) 2018-11-08 2021-06-15 贝克顿迪金森公司 使用随机引发的单细胞全转录组分析
JP7035972B2 (ja) * 2018-11-09 2022-03-15 横河電機株式会社 核酸配列計測用デバイス
CN113195717A (zh) 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
CN113574178B (zh) 2019-01-23 2024-10-29 贝克顿迪金森公司 与抗体关联的寡核苷酸
EP3924506A1 (de) 2019-02-14 2021-12-22 Becton Dickinson and Company Hybride gezielte und gesamttranskriptomamplifikation
CN113924041B (zh) 2019-03-14 2024-12-03 因斯利克萨公司 基于时间门控的荧光检测的方法和系统
EP3947718A4 (de) 2019-04-02 2022-12-21 Enumera Molecular, Inc. Verfahren, systeme und zusammensetzungen zum zählen von nukleinsäuremolekülen
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
ES2987640T3 (es) 2019-11-08 2024-11-15 Becton Dickinson Co Uso de cebado aleatorio para obtener información de V(D)J de longitud completa para la secuenciación del repertorio inmunitario
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
EP4097228B1 (de) 2020-01-29 2024-08-14 Becton, Dickinson and Company Barkodierte wells zur räumlichen abbildung einzelner zellen durch sequenzierung
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
US11149320B1 (en) 2020-03-31 2021-10-19 Diasorin S.P.A. Assays for the detection of SARS-CoV-2
EP4150118A1 (de) 2020-05-14 2023-03-22 Becton Dickinson and Company Primer zur profilierung von immunrepertoire
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116194589A (zh) 2020-07-31 2023-05-30 贝克顿迪金森公司 用于转座酶可及染色质的单细胞测定
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
WO2022132206A1 (en) 2020-12-15 2022-06-23 Becton, Dickinson And Company Single cell secretome analysis
EP4403645A4 (de) 2021-09-17 2025-10-08 Seegene Inc Nachweis einer zielnukleinsäuresequenz unter verwendung eines synthetischen, eine nichtnatürliche base tragenden markeroligonukleotids
EP4411386A4 (de) 2021-09-30 2025-10-08 Seegene Inc Verfahren zur verarbeitung und analyse einer probe in einem molekularen diagnostischen system
WO2023068823A1 (ko) 2021-10-21 2023-04-27 주식회사 씨젠 시료 내 표적 분석물질에 대한 양음성 판독 장치 및 방법
CN118382707B (zh) 2021-12-10 2025-04-25 日东纺绩株式会社 双链核酸的融解温度升高化剂及其用途
US20250305025A1 (en) 2022-05-06 2025-10-02 Seegene, Inc. Nucleic acid detection cartridge
EP4542552A1 (de) 2022-06-15 2025-04-23 Seegene, Inc. Verfahren und vorrichtung zum erstellen einer technischen konstruktionsdatei
WO2024010351A1 (ko) 2022-07-05 2024-01-11 주식회사 씨젠 복수의 표적 분석물 각각에 대한 근사 신호의 획득 방법 및 이를 수행하는 컴퓨터 장치
WO2024025390A1 (ko) 2022-07-29 2024-02-01 주식회사 씨젠 파라미터의 임계값 설정용 인터페이스 제공 장치 및 방법
EP4603593A1 (de) 2022-10-14 2025-08-20 Seegene, Inc. Verfahren zum nachweis von zielnukleinsäuren in einer probe unter verwendung von zwei sonden mit verschiedenen tm-werten
IL320357A (en) 2022-10-20 2025-06-01 Seegene Inc Method of guiding multiple detailed performance experiments required for developing reagent for detecting target nucleic acid molecule, and organizing experiment result record sheets
EP4621068A1 (de) 2022-11-16 2025-09-24 Seegene, Inc. Nachweis von zielnukleinsäuren durch quenching-pto-spaltungs- und verlängerungs-(q-ptoce)-assay
EP4636771A1 (de) 2022-12-15 2025-10-22 Seegene, Inc. Vorrichtung und verfahren zur anzeige des leistungsvergleichsergebnisses in bezug auf einen fluoreszenzdatenanalysealgorithmus
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5656493A (en) * 1985-03-28 1997-08-12 The Perkin-Elmer Corporation System for automated performance of the polymerase chain reaction
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
EP0533906A4 (en) * 1991-04-11 1994-09-28 Baxter Diagnostics Inc Detection of dna/rna by fluorescence polarization
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
US5746231A (en) * 1993-01-11 1998-05-05 Craig Lesser Tobacco smoke filter for removing toxic compounds
US5501238A (en) * 1993-01-11 1996-03-26 Von Borstel; Reid W. Cigarette filter containing a humectant
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5597696A (en) * 1994-07-18 1997-01-28 Becton Dickinson And Company Covalent cyanine dye oligonucleotide conjugates
US5817461A (en) * 1996-01-03 1998-10-06 Hamilton Civic Hospitals Research Development Inc. Methods and compositions for diagnosis of hyperhomocysteinemia
SE506700C2 (sv) * 1996-05-31 1998-02-02 Mikael Kubista Sond och förfaranden för analys av nukleinsyra
CA2274587A1 (en) * 1996-12-10 1998-06-18 Genetrace Systems Inc. Releasable nonvolatile mass-label molecules
US6451530B1 (en) * 1996-12-13 2002-09-17 The United States Of America As Represented By The Department Of Health And Human Services Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
US5846603A (en) * 1997-07-28 1998-12-08 Superior Fibers, Inc. Uniformly tacky filter media
JP2001514859A (ja) * 1997-09-04 2001-09-18 バイエル コーポレイション クエンチ可能な蛍光標識を有するオリゴヌクレオチドプローブおよびその使用方法
DE69841849D1 (de) * 1997-10-27 2010-09-30 Boston Probes Inc Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen
US5952202A (en) * 1998-03-26 1999-09-14 The Perkin Elmer Corporation Methods using exogenous, internal controls and analogue blocks during nucleic acid amplification
JP2003510017A (ja) 1999-06-22 2003-03-18 インビトロジェン コーポレイション 核酸の検出および識別のために改良されたプライマーおよび方法
SE9902565D0 (sv) * 1999-07-05 1999-07-05 Jonas Karlsson Probe for analysis of nucleic acids
FR2805348B1 (fr) * 2000-02-23 2002-07-12 Commissariat Energie Atomique Analyse de cibles biologiques utilisant une biopuce comportant un marqueur fluorescent
JP5213297B2 (ja) * 2000-08-11 2013-06-19 ユニバーシティ オブ ユタ リサーチ ファウンデーション 単一ラベルオリゴヌクレオチド・プローブ

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PT1715063E (pt) 2011-04-28
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