ATE485880T1 - Verfahren zur chromatographischen reinigung - Google Patents
Verfahren zur chromatographischen reinigungInfo
- Publication number
- ATE485880T1 ATE485880T1 AT05722292T AT05722292T ATE485880T1 AT E485880 T1 ATE485880 T1 AT E485880T1 AT 05722292 T AT05722292 T AT 05722292T AT 05722292 T AT05722292 T AT 05722292T AT E485880 T1 ATE485880 T1 AT E485880T1
- Authority
- AT
- Austria
- Prior art keywords
- chromatography matrix
- target compound
- purification method
- chromatographic purification
- ionic polyether
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000011097 chromatography purification Methods 0.000 title 1
- 239000012504 chromatography matrix Substances 0.000 abstract 3
- 239000004721 Polyphenylene oxide Substances 0.000 abstract 2
- 238000011210 chromatographic step Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- -1 poly(ethylene glycol) Polymers 0.000 abstract 2
- 229920000570 polyether Polymers 0.000 abstract 2
- 229920001223 polyethylene glycol Polymers 0.000 abstract 2
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/32—Bonded phase chromatography
- B01D15/325—Reversed phase
- B01D15/327—Reversed phase with hydrophobic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/26—Conditioning of the fluid carrier; Flow patterns
- G01N30/38—Flow patterns
- G01N30/46—Flow patterns using more than one column
- G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Polyethers (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0400886A SE0400886D0 (sv) | 2004-04-02 | 2004-04-02 | Process of purification |
| PCT/SE2005/000467 WO2005094960A1 (en) | 2004-04-02 | 2005-03-31 | A method for chromatographic purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE485880T1 true ATE485880T1 (de) | 2010-11-15 |
Family
ID=32173667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05722292T ATE485880T1 (de) | 2004-04-02 | 2005-03-31 | Verfahren zur chromatographischen reinigung |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20070213513A1 (OSRAM) |
| EP (1) | EP1729867B1 (OSRAM) |
| JP (1) | JP4831697B2 (OSRAM) |
| AT (1) | ATE485880T1 (OSRAM) |
| AU (1) | AU2005228834A1 (OSRAM) |
| CA (1) | CA2560012A1 (OSRAM) |
| DE (1) | DE602005024373D1 (OSRAM) |
| SE (1) | SE0400886D0 (OSRAM) |
| WO (1) | WO2005094960A1 (OSRAM) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| MX353340B (es) | 2006-09-13 | 2018-01-09 | Abbvie Inc | Mejoras de cultivos celulares. |
| JP2010530462A (ja) * | 2007-06-19 | 2010-09-09 | ジーイー・ヘルスケア・バイオ−サイエンシズ・アーベー | ポリマー２相システムおよびその使用 |
| AU2009204998B2 (en) | 2008-01-18 | 2014-03-27 | F. Hoffmann-La Roche Ag | Purification of non-glycosylated proteins |
| JP5596560B2 (ja) * | 2008-02-05 | 2014-09-24 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | 分離媒体の製造方法 |
| FR2930780B1 (fr) * | 2008-04-30 | 2010-08-20 | Moet & Chandon | Procede de degorgement de vins mousseux |
| US20090318674A1 (en) * | 2008-06-03 | 2009-12-24 | Gagnon Peter S | Process for purification of antibodies |
| TW201024318A (en) * | 2008-10-20 | 2010-07-01 | Abbott Lab | Isolation and purification of antibodies using protein A affinity chromatography |
| SG195577A1 (en) | 2008-10-20 | 2013-12-30 | Abbott Lab | Viral inactivation during purification of antibodies |
| ES2656167T5 (es) | 2011-03-16 | 2021-03-26 | Hoffmann La Roche | Cromatografía de intercambio iónico con selectividad mejorada para la separación de monómeros, agregados y fragmentos polipeptídicos mediante la modulación de la fase móvil |
| JP6147726B2 (ja) | 2011-03-29 | 2017-06-14 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニーＧｌａｘｏＳｍｉｔｈＫｌｉｎｅ ＬＬＣ | タンパク質精製のためのバッファー系 |
| KR101901830B1 (ko) * | 2011-06-08 | 2018-09-27 | 에이전시 포 사이언스, 테크놀로지 앤드 리서치 | 제한된 공수화 크로마토그래피를 통한 생물학적 산물의 정제 |
| EP2682168A1 (en) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Purification of biological molecules |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| JPWO2015041218A1 (ja) * | 2013-09-17 | 2017-03-02 | 株式会社カネカ | 新規抗体精製方法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎＭｅｔｈｏｄａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ）、並びに陽イオン交換基を用いた新規抗体精製法及びそれから得られる抗体（ＮｏｖｅｌＡｎｔｉｂｏｄｙＰｕｒｉｆｉｃａｔｉｏｎｍｅｔｈｏｄｕｓｉｎｇＣａｔｉｏｎＥｘｃｈａｎｇｅｒａｎｄＡｎｔｉｂｏｄｙｏｂｔａｉｎｅｄｔｈｅｒｅｆｒｏｍ） |
| TW201628649A (zh) | 2014-10-09 | 2016-08-16 | 再生元醫藥公司 | 減少醫藥調配物中微可見顆粒之方法 |
| KR20230041834A (ko) | 2016-08-16 | 2023-03-24 | 리제너론 파아마슈티컬스, 인크. | 혼합물로부터 개별 항체들을 정량하는 방법 |
| CN119492842A (zh) | 2016-10-25 | 2025-02-21 | 里珍纳龙药品有限公司 | 用于色谱数据分析的方法和系统 |
| CN118416215A (zh) | 2017-09-19 | 2024-08-02 | 里珍纳龙药品有限公司 | 减少粒子形成的方法以及由其形成的组合物 |
| CN108152104B (zh) * | 2017-12-25 | 2020-08-11 | 上海微谱化工技术服务有限公司 | 超支化水性聚氨酯中二氧化硅填料的分离及定量方法 |
| WO2019183334A1 (en) | 2018-03-21 | 2019-09-26 | Waters Technologies Corporation | Non-antibody high-affinity-based sample preparation, sorbents, devices and methods |
| WO2019237100A1 (en) * | 2018-06-08 | 2019-12-12 | Board Of Regents, The University Of Texas System | Systems and methods for separation of olefins from mixtures that contain reducing agents |
| TWI853823B (zh) | 2018-07-02 | 2024-09-01 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| EP3934620A1 (en) | 2019-03-05 | 2022-01-12 | Regeneron Pharmaceuticals, Inc. | Human serum albumin in formulations |
| EP4038082A1 (en) * | 2019-10-04 | 2022-08-10 | Merck Patent GmbH | Elution of monoclonal antibodies in protein a affinity chromatography |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3869436A (en) * | 1971-06-01 | 1975-03-04 | Statens Bakteriologiska Lab | Method for fractionating plasma proteins using peg and ion-exchangers |
| EP0119767B1 (en) * | 1983-03-11 | 1990-11-22 | FUJIREBIO KABUSHIKI KAISHA also trading as FUJIREBIO INC. | Method of measuring ligands |
| US4683294A (en) * | 1985-04-03 | 1987-07-28 | Smith Kline Rit, S.A. | Process for the extraction and purification of proteins from culture media producing them |
| DE3789413T2 (de) * | 1986-10-03 | 1994-07-28 | Ciba Geigy Ag | Lymphokin-ähnliche Peptide. |
| US5151358A (en) * | 1989-06-13 | 1992-09-29 | Genencor International, Inc. | Processes for the recovery of naturally produced chymosin |
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US5443953A (en) * | 1993-12-08 | 1995-08-22 | Immunomedics, Inc. | Preparation and use of immunoconjugates |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| SE9503925D0 (sv) * | 1995-11-07 | 1995-11-07 | Pharmacia Biotech Ab | Separationsmedium för IgG |
| WO1998017312A1 (en) * | 1996-10-22 | 1998-04-30 | Chugai Seiyaku Kabushiki Kaisha | Sepsis remedy comprising anti-il-8 antibody as active ingredient |
| AUPR110100A0 (en) * | 2000-10-30 | 2000-11-23 | Life Therapeutics Limited | Improved separation of macromolecules |
| US7514078B2 (en) * | 2001-06-01 | 2009-04-07 | Cornell Research Foundation, Inc. | Methods of treating prostate cancer with anti-prostate specific membrane antigen antibodies |
| US7998705B2 (en) * | 2002-08-06 | 2011-08-16 | FUJIFILM Diosynth Biotechnologies U.S.A., Inc | Increased dynamic binding capacity in ion exchange chromatography by addition of polyethylene glycol |
| US20060275766A1 (en) * | 2003-01-07 | 2006-12-07 | Haurum John S | Method for manufacturing recombinant polyclonal proteins |
-
2004
- 2004-04-02 SE SE0400886A patent/SE0400886D0/xx unknown
-
2005
- 2005-03-31 US US10/594,187 patent/US20070213513A1/en not_active Abandoned
- 2005-03-31 AT AT05722292T patent/ATE485880T1/de not_active IP Right Cessation
- 2005-03-31 EP EP05722292A patent/EP1729867B1/en not_active Expired - Lifetime
- 2005-03-31 JP JP2007506111A patent/JP4831697B2/ja not_active Expired - Lifetime
- 2005-03-31 DE DE602005024373T patent/DE602005024373D1/de not_active Expired - Lifetime
- 2005-03-31 WO PCT/SE2005/000467 patent/WO2005094960A1/en not_active Ceased
- 2005-03-31 AU AU2005228834A patent/AU2005228834A1/en not_active Abandoned
- 2005-03-31 CA CA002560012A patent/CA2560012A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005094960A1 (en) | 2005-10-13 |
| DE602005024373D1 (de) | 2010-12-09 |
| EP1729867A1 (en) | 2006-12-13 |
| EP1729867B1 (en) | 2010-10-27 |
| JP4831697B2 (ja) | 2011-12-07 |
| JP2007530971A (ja) | 2007-11-01 |
| SE0400886D0 (sv) | 2004-04-02 |
| US20070213513A1 (en) | 2007-09-13 |
| AU2005228834A1 (en) | 2005-10-13 |
| CA2560012A1 (en) | 2005-10-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |