ATE403674T1 - Neuer assay für die auftrennung und quantifizierung von hämagglutinin-antigenen - Google Patents

Neuer assay für die auftrennung und quantifizierung von hämagglutinin-antigenen

Info

Publication number
ATE403674T1
ATE403674T1 AT05716900T AT05716900T ATE403674T1 AT E403674 T1 ATE403674 T1 AT E403674T1 AT 05716900 T AT05716900 T AT 05716900T AT 05716900 T AT05716900 T AT 05716900T AT E403674 T1 ATE403674 T1 AT E403674T1
Authority
AT
Austria
Prior art keywords
antigens
methods
quantification
separation
column
Prior art date
Application number
AT05716900T
Other languages
English (en)
Inventor
Fija Lagerwerf
Johan Kapteyn
Original Assignee
Crucell Holland Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crucell Holland Bv filed Critical Crucell Holland Bv
Application granted granted Critical
Publication of ATE403674T1 publication Critical patent/ATE403674T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
    • C12N2760/16151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16311—Influenzavirus C, i.e. influenza C virus
    • C12N2760/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16311—Influenzavirus C, i.e. influenza C virus
    • C12N2760/16351—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/11—Orthomyxoviridae, e.g. influenza virus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/115—Paramyxoviridae, e.g. parainfluenza virus
    • G01N2333/12—Mumps virus; Measles virus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT05716900T 2004-03-17 2005-03-03 Neuer assay für die auftrennung und quantifizierung von hämagglutinin-antigenen ATE403674T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP2004050318 2004-03-17

Publications (1)

Publication Number Publication Date
ATE403674T1 true ATE403674T1 (de) 2008-08-15

Family

ID=34957414

Family Applications (1)

Application Number Title Priority Date Filing Date
AT05716900T ATE403674T1 (de) 2004-03-17 2005-03-03 Neuer assay für die auftrennung und quantifizierung von hämagglutinin-antigenen

Country Status (10)

Country Link
US (3) US7638608B2 (de)
EP (1) EP1725581B1 (de)
AT (1) ATE403674T1 (de)
AU (1) AU2005223371B2 (de)
CA (1) CA2560185C (de)
DE (1) DE602005008722D1 (de)
DK (1) DK1725581T3 (de)
ES (1) ES2313310T3 (de)
NZ (1) NZ549776A (de)
WO (1) WO2005090390A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100347306C (zh) * 2001-10-29 2007-11-07 克鲁塞尔荷兰公司 生产具有预定的翻译后修饰的蛋白质的方法和手段
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
WO2005056752A2 (en) 2003-10-24 2005-06-23 Gencia Corporation Methods and compositions for delivering polynucleotides
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
WO2005090390A1 (en) 2004-03-17 2005-09-29 Crucell Holland B.V. Novel assay for the separation and quantification of hemagglutinin antigens
AU2006243854A1 (en) * 2005-05-02 2006-11-09 Crucell Holland B.V. Novel assay for the separation and quantification of hemagglutinin antigens
US8530182B2 (en) * 2007-12-05 2013-09-10 Centers For Disease Control And Prevention Viral protein quantification process and vaccine quality control therewith
WO2009085106A1 (en) * 2007-12-27 2009-07-09 Duke University Resin assisted capture of cysteine-modified proteins/peptides and determination of presence and location of modification
CA2741961A1 (en) * 2008-11-05 2010-05-14 Glaxosmithkline Biologicals S.A. Novel method
US20120237545A1 (en) * 2009-05-29 2012-09-20 Norvartis Ag Assays for influenza virus hemagglutinins
CA2774555A1 (en) * 2009-09-25 2011-03-31 Glaxosmithkline Biologicals S.A. Immunodiffusion assay for influenza virus
KR101960928B1 (ko) 2011-04-21 2019-07-17 올로지 바이오서비시즈, 인크. 백신으로부터 항원을 단리하고 정량하기 위한 방법
EP3023790A1 (de) 2011-12-12 2016-05-25 Novartis AG Assay für influenzavirus-hämagglutinine
US10732180B2 (en) 2014-06-04 2020-08-04 Indevr, Inc. Universal capture array for multiplexed subtype-specific quantification and stability determination of influenza proteins
CA2957570A1 (en) * 2014-08-18 2016-02-25 Sanofi Pasteur Inc. Alkylated influenza vaccines
WO2017005880A1 (en) * 2015-07-07 2017-01-12 Seqirus UK Limited Influenza potency assays
AU2021264292A1 (en) * 2020-04-29 2022-12-08 Sk Bioscience Co., Ltd. Influenza virus production method using single-use culture process system and rapid confirmation test of influenza virus antigen purification condition

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100347306C (zh) * 2001-10-29 2007-11-07 克鲁塞尔荷兰公司 生产具有预定的翻译后修饰的蛋白质的方法和手段
WO2005090390A1 (en) 2004-03-17 2005-09-29 Crucell Holland B.V. Novel assay for the separation and quantification of hemagglutinin antigens
US7449116B2 (en) * 2004-10-01 2008-11-11 Agilent Technologies, Inc. Methods and systems for protein separation

Also Published As

Publication number Publication date
US20100041022A1 (en) 2010-02-18
EP1725581B1 (de) 2008-08-06
US20060051742A1 (en) 2006-03-09
CA2560185C (en) 2013-05-07
US20070196387A1 (en) 2007-08-23
DE602005008722D1 (de) 2008-09-18
CA2560185A1 (en) 2005-09-29
WO2005090390A1 (en) 2005-09-29
US7638608B2 (en) 2009-12-29
EP1725581A1 (de) 2006-11-29
AU2005223371A1 (en) 2005-09-29
AU2005223371B2 (en) 2011-06-09
NZ549776A (en) 2009-07-31
US7687611B2 (en) 2010-03-30
DK1725581T3 (da) 2008-11-10
ES2313310T3 (es) 2009-03-01
US8283452B2 (en) 2012-10-09

Similar Documents

Publication Publication Date Title
DE602005008722D1 (de) Neuer assay für die auftrennung und quantifizierung von hämagglutinin-antigenen
Slentz et al. Protein proteolysis and the multi-dimensional electrochromatographic separation of histidine-containing peptide fragments on a chip
Wolters et al. An automated multidimensional protein identification technology for shotgun proteomics
Guo et al. Recent advances in bio-affinity chromatography for screening bioactive compounds from natural products
Delaunay-Bertoncini et al. Immunoaffinity solid-phase extraction for pharmaceutical and biomedical trace-analysis—coupling with HPLC and CE—perspectives
Gaillard et al. Poisoning by plant material: review of human cases and analytical determination of main toxins by high-performance liquid chromatography–(tandem) mass spectrometry
Lamprecht et al. Enantioselective analysis of (R)-and (S)-atenolol in urine samples by a high-performance liquid chromatography column-switching setup
ATE529752T1 (de) Antikörperquantifizierung
Masuda et al. Microscale phosphoproteome analysis of 10 000 cells from human cancer cell lines
WO2005121163A3 (en) Isolation of plasma or serum proteins
Liang et al. Bridged hybrid monolithic column coupled to high-resolution mass spectrometry for top-down proteomics
Jmeian et al. Tandem Affinity Monolithic Microcolumns with Immobilized Protein A, Protein G ‘, and Antibodies for Depletion of High Abundance Proteins from Serum Samples: Integrated Microcolumn-Based Fluidic System for Simultaneous Depletion and Tryptic Digestion
Ma et al. A simple way to configure on-line two-dimensional liquid chromatography for complex sample analysis: acquisition of four-dimensional data
Ding et al. Selective enrichment of glycopeptides from glycoprotein digests using ion-pairing normal-phase liquid chromatography
Xiong et al. Potential of silica monolithic columns in peptide separations
Chaves et al. Biocompatible in-tube solid phase microextraction coupled with liquid chromatography-fluorescence detection for determination of interferon α in plasma samples
Yuan et al. Integrated platform for proteome profiling with combination of microreversed phase based protein and peptide separation via online solvent exchange and protein digestion
Singer et al. Separation of multiphosphorylated peptide isomers by hydrophilic interaction chromatography on an aminopropyl phase
Emmett et al. Supercritical fluid chromatography reduction of hydrogen/deuterium back exchange in solution-phase hydrogen/deuterium exchange with mass spectrometric analysis
Rucevic et al. Use of short monolithic columns for isolation of low abundance membrane proteins
Asensio-Ramos et al. Nano-liquid chromatography
WO2005026323A3 (en) Stationary phases and a purification process using the stationary phases
De Vos et al. Prototyping of a microfluidic modulator chip and its application in heart-cut strong-cation-exchange-reversed-phase liquid chromatography coupled to nanoelectrospray mass spectrometry for targeted proteomics
Brgles et al. Selectivity of monolithic supports under overloading conditions and their use for separation of human plasma and isolation of low abundance proteins
Hu et al. Separation methods applied to arsenic speciation

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1725581

Country of ref document: EP