ATE449844T1 - Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems - Google Patents

Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Info

Publication number
ATE449844T1
ATE449844T1 AT02021915T AT02021915T ATE449844T1 AT E449844 T1 ATE449844 T1 AT E449844T1 AT 02021915 T AT02021915 T AT 02021915T AT 02021915 T AT02021915 T AT 02021915T AT E449844 T1 ATE449844 T1 AT E449844T1
Authority
AT
Austria
Prior art keywords
new method
host cell
homologous recombination
recombination system
cloning dna
Prior art date
Application number
AT02021915T
Other languages
German (de)
English (en)
Inventor
Francis Stewart
Youming Zhang
Frank Buchholz
Original Assignee
Europ Lab Molekularbiolog
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europ Lab Molekularbiolog filed Critical Europ Lab Molekularbiolog
Application granted granted Critical
Publication of ATE449844T1 publication Critical patent/ATE449844T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02021915T 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems ATE449844T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97121462 1997-12-05
EP98118756 1998-10-05

Publications (1)

Publication Number Publication Date
ATE449844T1 true ATE449844T1 (de) 2009-12-15

Family

ID=26145961

Family Applications (2)

Application Number Title Priority Date Filing Date
AT02021915T ATE449844T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
AT98963541T ATE242320T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT98963541T ATE242320T1 (de) 1997-12-05 1998-12-07 Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems

Country Status (11)

Country Link
US (5) US6509156B1 (https=)
EP (2) EP1034260B1 (https=)
JP (1) JP4139561B2 (https=)
AT (2) ATE449844T1 (https=)
AU (1) AU752105B2 (https=)
CA (2) CA2759882A1 (https=)
DE (2) DE69815384T2 (https=)
DK (1) DK1034260T3 (https=)
ES (2) ES2201567T3 (https=)
PT (1) PT1034260E (https=)
WO (1) WO1999029837A2 (https=)

Families Citing this family (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6156574A (en) 1997-06-23 2000-12-05 The Rockefeller University Methods of performing gene trapping in bacterial and bacteriophage-derived artificial chromosomes and use thereof
US6821759B1 (en) 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US6509156B1 (en) * 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system
AUPP684998A0 (en) * 1998-10-30 1998-11-26 Murdoch Institute for Research into Birth Defects Limited, The A method of recombination and agents useful for same
US6720139B1 (en) 1999-01-27 2004-04-13 Elitra Pharmaceuticals, Inc. Genes identified as required for proliferation in Escherichia coli
US6709852B1 (en) 1999-06-22 2004-03-23 Invitrogen Corporation Rapid growing microorganisms for biotechnology applications
CN1192101C (zh) * 1999-06-25 2005-03-09 惠氏控股有限公司 革兰阴性菌的肽聚糖结合脂蛋白的脂化形式的制备
US6355412B1 (en) * 1999-07-09 2002-03-12 The European Molecular Biology Laboratory Methods and compositions for directed cloning and subcloning using homologous recombination
AU6229800A (en) * 1999-07-20 2001-02-05 Rockefeller University, The Conditional homologous recombination of large genomic vector inserts
US6589738B1 (en) 1999-11-09 2003-07-08 Elitra Pharmaceuticals, Inc. Genes essential for microbial proliferation and antisense thereto
DK1311661T3 (da) 2000-08-14 2012-11-26 Us Gov Health & Human Serv Forøget homolog rekombination medieret ved lambda-rekombinationsproteiner
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002062988A2 (en) * 2001-02-09 2002-08-15 Gene Bridges Gmbh Recombination method
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
EP1409702A1 (en) * 2001-07-20 2004-04-21 Youming Zhang Improved rect or recet cloning and subcloning method
WO2003010333A2 (en) * 2001-07-24 2003-02-06 Affinium Pharmaceuticals Inc. Methods for gene disruption and uses thereof
US7338800B2 (en) * 2002-01-16 2008-03-04 Baylor College Of Medicine In vivo gene transfer
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
US20060270043A1 (en) * 2002-01-23 2006-11-30 Blattner Frederick R Bacteria with reduced genome
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US8039243B2 (en) * 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
US8119365B2 (en) * 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
AU2003257155A1 (en) * 2002-08-02 2004-02-23 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
DE10236001A1 (de) 2002-08-06 2004-02-19 Icon Genetics Ag Transformation von Plastiden unter Verwendung modularer Vektoren
US7303906B2 (en) * 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
US7563600B2 (en) * 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US20040209370A1 (en) * 2002-12-19 2004-10-21 Wonchul Suh Method for chromosomal engineering
US7521242B2 (en) 2003-05-09 2009-04-21 The United States Of America As Represented By The Department Of Health And Human Services Host cells deficient for mismatch repair and their use in methods for inducing homologous recombination using single-stranded nucleic acids
WO2005014796A2 (en) 2003-08-08 2005-02-17 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
WO2005054438A2 (en) * 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
AU2005222788A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
JP2005245320A (ja) * 2004-03-04 2005-09-15 Aisin Seiki Co Ltd 核酸組換え方法、宿主細胞、及び、発現ベクター
US7674621B2 (en) 2004-05-21 2010-03-09 The United States Of America As Represented By The Department Of Health And Human Services Plasmids and phages for homologous recombination and methods of use
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
JP2008523786A (ja) * 2004-10-18 2008-07-10 コドン デバイシズ インコーポレイテッド 高忠実度合成ポリヌクレオチドのアセンブリ方法
GB0423755D0 (en) * 2004-10-26 2004-11-24 Gene Bridges Gmbh Methods for heterologus expression of secondary metabolites
US20080051326A1 (en) * 2004-11-12 2008-02-28 Alexander Dylan C Antiinfective Lipopeptides
WO2006127423A2 (en) * 2005-05-18 2006-11-30 Codon Devices, Inc. Methods of producing polynucleotide libraries using scarless ligation
WO2007005053A1 (en) * 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
WO2007110695A2 (en) * 2005-10-13 2007-10-04 Bc Cancer Agency Modular genomes for synthetic biology and metabolic engineering
KR100681815B1 (ko) 2005-12-02 2007-02-12 한국생명공학연구원 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법.
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
ES2465996T3 (es) * 2006-05-25 2014-06-09 Sangamo Biosciences, Inc. Métodos y composiciones para la inactivación genética
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
WO2008142028A1 (en) 2007-05-17 2008-11-27 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
GB0803109D0 (en) * 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
EP2251423B1 (en) 2008-03-07 2016-01-13 National University Corporation University Of Toyama Homologous recombination method, cloning method, and kit
WO2010030776A1 (en) * 2008-09-10 2010-03-18 Genscript Corporation Homologous recombination-based dna cloning methods and compositions
GB0909660D0 (en) 2009-06-04 2009-07-22 Gene Bridges Gmbh Method of altering nucleic acids
DK3622815T3 (da) 2009-07-08 2023-06-26 Kymab Ltd Gnavermodeller og terapeutiske molekyler
WO2011053957A2 (en) 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
US20110125118A1 (en) * 2009-11-20 2011-05-26 Opx Biotechnologies, Inc. Production of an Organic Acid and/or Related Chemicals
GB0922108D0 (en) 2009-12-17 2010-02-03 Gene Bridges Gmbh Heterologous hosts
WO2011080505A2 (en) 2009-12-28 2011-07-07 Bigdna Ltd Method of growing phage for commercial use
JP2011217736A (ja) * 2010-03-26 2011-11-04 Aichi Medical Univ 組換えクラミジア及びその製造方法
US9096825B2 (en) 2010-05-27 2015-08-04 Pharmazell Gmbh 7 α-hydroxysteroid dehydrogenase knockout mutants and use therefor
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
CN103228130B (zh) 2010-06-17 2016-03-16 科马布有限公司 动物模型及治疗分子
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
CN103502448B (zh) 2010-11-12 2017-03-29 Gen9股份有限公司 核酸合成的方法和设备
CA2868113A1 (en) 2011-03-22 2012-09-27 Opx Biotechnologies, Inc. Microbial production of chemical products and related compositions, methods and systems
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
BR112014006394A2 (pt) 2011-09-19 2017-03-28 Kymab Ltd manipulação de diversidade genética de imunoglobulina e terapêuticos multi-anticorpos
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN107058059B (zh) 2011-09-26 2020-08-07 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US9253965B2 (en) 2012-03-28 2016-02-09 Kymab Limited Animal models and therapeutic molecules
CN103160495A (zh) * 2011-12-15 2013-06-19 张力军 一种非连接依赖性的快速克隆方法
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN102634534A (zh) * 2012-03-30 2012-08-15 深圳市中联生物科技开发有限公司 基于同源重组的核酸分子克隆方法及相关试剂盒
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
US20130288320A1 (en) 2012-04-27 2013-10-31 Bioamber Inc. Methods and microorganisms for increasing the biological synthesis of difunctional alkanes
CN102676470B (zh) * 2012-05-29 2014-04-16 吴江近岸蛋白质科技有限公司 一种用于快速基因克隆的酶混合物及制备方法、包含该酶混合物的试剂盒
IL236303B (en) 2012-06-25 2022-07-01 Gen9 Inc Methods for high-throughput nucleic acid assembly and sequencing
US20150353940A1 (en) * 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
SI2959006T1 (sl) 2013-02-22 2018-10-30 F. Hoffmann-La Roche Ag Uporaba prokariontskega seva, ozdravljenega aminokislinske avksotrofije, za proizvodnjo rekombinantnega polipeptida
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
AU2015324564B2 (en) 2014-06-11 2021-07-01 Duke University Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
US10407676B2 (en) 2014-12-09 2019-09-10 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN104630178A (zh) * 2015-01-28 2015-05-20 苏州神洲基因有限公司 一种混合蛋白酶体系、包含该混合蛋白酶体系的试剂盒及其使用方法
BR112017021667A2 (pt) 2015-04-09 2018-07-24 Genomatica Inc microorganismos modificados & métodos para produção aperfeiçoada de álcool de crotila
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
JP2020530979A (ja) 2017-06-30 2020-11-05 インスクリプタ， インコーポレイテッド 自動細胞処理方法、モジュール、機器及びシステム
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
JP6985508B2 (ja) 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US10443031B1 (en) 2018-03-29 2019-10-15 Inscripta, Inc. Methods for controlling the growth of prokaryotic and eukaryotic cells
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
WO2020081149A2 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
US20220228157A1 (en) * 2019-05-23 2022-07-21 President And Fellows Of Harvard College Gene editing in diverse bacteria
WO2020257395A1 (en) 2019-06-21 2020-12-24 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
BR112022017196A2 (pt) * 2020-03-03 2022-10-25 Univ Leland Stanford Junior Recombinação genômica guiada por rna na escala de kilobase
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN115160416B (zh) * 2022-06-08 2025-06-10 青岛农业大学 一种感应潜艇金属离子Cd(II)的AraC突变体及其构建的探潜微生物传感器和应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4963487A (en) * 1985-01-03 1990-10-16 Massachusetts Institute Of Technology Method for deletion of a gene from a bacteria
US5286632A (en) * 1991-01-09 1994-02-15 Jones Douglas H Method for in vivo recombination and mutagenesis
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6509156B1 (en) * 1997-12-05 2003-01-21 Europaisches Laboratorium Fur Molekularoiologie (Embl) DNA cloning method relying on the E. coli recE/recT recombination system

Also Published As

Publication number Publication date
US20030036198A1 (en) 2003-02-20
EP1034260B1 (en) 2003-06-04
EP1034260A2 (en) 2000-09-13
US20120208277A1 (en) 2012-08-16
AU1877199A (en) 1999-06-28
WO1999029837A3 (en) 1999-07-22
ES2201567T3 (es) 2004-03-16
US20040203057A1 (en) 2004-10-14
US20100317061A1 (en) 2010-12-16
DE69815384D1 (de) 2003-07-10
AU752105B2 (en) 2002-09-05
US7736851B2 (en) 2010-06-15
CA2759882A1 (en) 1999-06-17
EP1291420B1 (en) 2009-11-25
JP4139561B2 (ja) 2008-08-27
WO1999029837B1 (en) 1999-09-16
PT1034260E (pt) 2003-10-31
WO1999029837A2 (en) 1999-06-17
ES2337027T3 (es) 2010-04-20
US6509156B1 (en) 2003-01-21
CA2312474A1 (en) 1999-06-17
ATE242320T1 (de) 2003-06-15
DK1034260T3 (da) 2003-09-29
CA2312474C (en) 2012-07-03
EP1291420A1 (en) 2003-03-12
DE69815384T2 (de) 2003-12-04
DE69841340D1 (de) 2010-01-07
US6787316B2 (en) 2004-09-07
JP2002503448A (ja) 2002-02-05

Similar Documents

Publication Publication Date Title
ATE449844T1 (de) Neue methode zur klonierung dns unter anwendung des e. coli rece/rect rekombinationssystems
ES2525677T3 (es) Celulasas, genes que las codifican y usos de las mismas
PT698105E (pt) Sequencias de nucleotidos para o controlo da expressao de sequencias de adn num hospedeiro celular
BR9814404A (pt) " clonagem de expressão em fungos filamentosos "
ATE415480T1 (de) B7l-1 moleküle
WO1999000410A3 (en) Human extracellular matrix proteins
EP0947142A3 (en) Method for cross-linking protein by using enzyme
NZ510356A (en) Molecules designated LDCAM
FI961196A0 (fi) Aktiviinin reseptoreita muistuttava kinaasi(alk), joka kuuluu TGF-reseptoriperheeseen ja/tai BMP-reseptoriperheeseen
BR9712942A (pt) Método para a produção de glicerol, célula hospedeira transformada, método para a seleção da expressão do gene, cepa pah21/dh5x de escherichia coli e cepa pah21/dh5x de escherichia coli.
DK0763101T3 (da) Nukleinsyresekvenser for og ekspressionssystemer til heparinase III afledt af Flavobacterium heparinum
CY1106978T1 (el) Ενζυμα φωσφοδιεστερασης
NO177570C (no) Fremgangsmåte ved fremstilling av peptider med fibronektinbindende egenskaper, mikroorganisme og DNA-sekvens som koder for peptider med fibronektinbindende egenskaper
DK0956150T3 (da) Uorganisk nanofiltreringsmembran og dens anvendelse i sukkerindustrien
DK2386319T3 (da) Promotere, der udviser endothelcellespecificitet og fremgangsmåder til anvendelse heraf
DE69532725D1 (de) Methode zur direkter klonierung von nukleasegenen in e.coli
DE69533626D1 (de) Tioredioxine h aus zwig und hart-weizen und ähnliche proteine, diese kodierende dna-fragmente und verfahren zu ihrer herstellung
EA199900893A1 (ru) Бактериальные плазмиды
DK0923644T3 (da) G-koblet receptor, der udviser selektiv selektivitet for ATP
EE200100234A (et) PRV-1-geen ja selle kasutamine
DK1129191T3 (da) Fremgangsmåder til diagnosticering eller behandling af neurologiske sygdomme
DK1127943T3 (da) Midler og fremgangsmåder til ekspression af homologe og heterologe proteiner i stammer af Rhodococcus
WO2003022039A1 (fr) Procede permettant de conferer une resistance a un virus au moyen d'une proteine vegetale se liant a une proteine de transport de virus vegetal
DK1141310T3 (da) Produktion af proteiner i grampositive mikroorganismer
JPH10210974A5 (https=)

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties