ATE397208T1 - Messverfahren basierend auf fluoreszenz- energie- transfer mit einem donor langer fluoreszenzlebenszeit - Google Patents

Messverfahren basierend auf fluoreszenz- energie- transfer mit einem donor langer fluoreszenzlebenszeit

Info

Publication number
ATE397208T1
ATE397208T1 AT01908141T AT01908141T ATE397208T1 AT E397208 T1 ATE397208 T1 AT E397208T1 AT 01908141 T AT01908141 T AT 01908141T AT 01908141 T AT01908141 T AT 01908141T AT E397208 T1 ATE397208 T1 AT E397208T1
Authority
AT
Austria
Prior art keywords
fluorescence
energy transfer
donor
method based
measuring method
Prior art date
Application number
AT01908141T
Other languages
English (en)
Inventor
Tetsuo Nagano
Kazuya Kikuchi
Mitsunori Koresawa
Hirotatsu Kojima
Original Assignee
Daiichi Pure Chemicals Co Ltd
Tetsuo Nagano
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Daiichi Pure Chemicals Co Ltd, Tetsuo Nagano filed Critical Daiichi Pure Chemicals Co Ltd
Application granted granted Critical
Publication of ATE397208T1 publication Critical patent/ATE397208T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
AT01908141T 2000-02-28 2001-02-28 Messverfahren basierend auf fluoreszenz- energie- transfer mit einem donor langer fluoreszenzlebenszeit ATE397208T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000050868 2000-02-28

Publications (1)

Publication Number Publication Date
ATE397208T1 true ATE397208T1 (de) 2008-06-15

Family

ID=18572606

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01908141T ATE397208T1 (de) 2000-02-28 2001-02-28 Messverfahren basierend auf fluoreszenz- energie- transfer mit einem donor langer fluoreszenzlebenszeit

Country Status (10)

Country Link
US (2) US20030157727A1 (de)
EP (1) EP1271133B1 (de)
JP (1) JP4589588B2 (de)
KR (1) KR20030011777A (de)
CN (1) CN1222767C (de)
AT (1) ATE397208T1 (de)
AU (2) AU3599601A (de)
CA (1) CA2401360A1 (de)
DE (1) DE60134219D1 (de)
WO (1) WO2001063265A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030010585A (ko) 2000-02-28 2003-02-05 다이이치 가가쿠 야쿠힝 가부시키가이샤 아연 형광탐침
US6756231B1 (en) * 2000-08-18 2004-06-29 Daiichi Pure Chemicals Co., Ltd. Diaminorhodamine derivative
EP1553408A4 (de) 2002-07-08 2005-08-17 Tetsuo Nagano Fluoreszenzsonde
WO2004040296A1 (ja) * 2002-10-16 2004-05-13 Daiichi Pure Chemicals Co., Ltd. パーオキシナイトライト測定用試薬
US7696245B2 (en) 2003-03-28 2010-04-13 Sekisui Medical Co., Ltd. Fluorescent probe for zinc
US7378282B2 (en) 2003-05-14 2008-05-27 Tetsuo Nagano Method for measuring hypochlorite ion
DE10337108B3 (de) * 2003-08-11 2005-05-04 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Photochromes relaxationskinetisches Verfahren
US7943395B2 (en) 2003-11-21 2011-05-17 Kimberly-Clark Worldwide, Inc. Extension of the dynamic detection range of assay devices
US20050112703A1 (en) 2003-11-21 2005-05-26 Kimberly-Clark Worldwide, Inc. Membrane-based lateral flow assay devices that utilize phosphorescent detection
JP2005194244A (ja) * 2004-01-09 2005-07-21 Shigenobu Yano 亜鉛イオン蛍光センサー
US20100255518A1 (en) * 2006-04-04 2010-10-07 Goix Philippe J Highly sensitive system and methods for analysis of troponin
WO2009107769A1 (ja) * 2008-02-29 2009-09-03 国立大学法人東京大学 活性酸素測定用試薬
JP2010129634A (ja) * 2008-11-26 2010-06-10 Tokyo Electron Ltd 基板の保管装置及び基板の処理装置
CN103881707A (zh) * 2013-12-30 2014-06-25 安徽师范大学 一种磷光能量转移体系，其合成方法，用途以及单链脱氧核糖核苷酸的检测方法
CN104267006B (zh) * 2014-08-21 2016-06-01 北京师范大学 邻香豆酸-LTbH复合体及其合成方法
CN104316501A (zh) * 2014-10-20 2015-01-28 南昌大学 基于荧光共振能量转移对硫氰酸根离子进行检测的方法
CN104359880B (zh) * 2014-11-05 2017-03-15 合肥学院 对痕量百草枯检测的CdTe量子点荧光探针的化学制备方法
CN104650334B (zh) * 2015-02-16 2016-08-24 上海交通大学 可降解的项链状一氧化氮荧光高分子探针的制备方法
CN106290268A (zh) * 2016-07-12 2017-01-04 华南师范大学 一种利用单个串联供体受体结构测量受体‑供体消光系数比值的方法
CN107163068A (zh) * 2017-05-08 2017-09-15 辽宁大学 一种新型荧光探针及其制备方法和在检测尿酸中的应用
CN111855626B (zh) * 2020-06-29 2022-08-12 中国科学院苏州生物医学工程技术研究所 一种时间分辨荧光共振能量转移体系及其应用

Family Cites Families (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US43498A (en) * 1864-07-12 Improved cooling-tub for water and beer
US182253A (en) * 1876-09-12 Improvement in the methods of forming last-blanks
US157727A (en) * 1874-12-15 Improvement in water-indicators for steam-boilers
US64308A (en) * 1867-04-30 Assifinqftrto himself
US30054A (en) * 1860-09-18 Improvement sn molds for rifle-balls
US162298A (en) * 1875-04-20 Improvement in sight-protectors
US130314A (en) * 1872-08-06 Improvement in wheels for vehicles
US37332A (en) * 1863-01-06 Improvement in machines for drawing bolts and spikes
US147035A (en) * 1874-02-03 Improvement in fertilizing compounds to be used to protect trees
US123478A (en) * 1872-02-06 Improvement in rotary cutters for sole-trimming machines
US153027A (en) * 1874-07-14 Improvement in egg and fruit carriers
GB8602304D0 (en) * 1986-01-30 1986-03-05 Dakubu S Dihydropyridine condensation products
US5049673A (en) * 1987-10-30 1991-09-17 The Regents Of The University Of California Fluorescent indicator dyes for calcium working at long wavelengths
US5037615A (en) * 1987-10-30 1991-08-06 Cordis Corporation Tethered pair fluorescence energy transfer indicators, chemical sensors, and method of making such sensors
US5648270A (en) * 1995-02-06 1997-07-15 Molecular Probes, Inc. Methods of sensing with fluorescent conjugates of metal-chelating nitrogen heterocycles
US5208148A (en) * 1990-12-07 1993-05-04 Molecular Probes, Inc. Lipophilic fluorescent glycosidase substrates
US5451343A (en) * 1991-05-20 1995-09-19 Spectra Group Limited, Inc. Fluorone and pyronin y derivatives
DE69233674T2 (de) * 1991-05-22 2007-10-18 Dade Behring Marburg Gmbh Homogenes Testsystem zur Bestimmung eines Analytes
US5340716A (en) * 1991-06-20 1994-08-23 Snytex (U.S.A.) Inc. Assay method utilizing photoactivated chemiluminescent label
US5246867A (en) * 1992-01-17 1993-09-21 University Of Maryland At Baltimore Determination and quantification of saccharides by luminescence lifetimes and energy transfer
US5302731A (en) * 1992-07-13 1994-04-12 Becton, Dickinson And Company Fluorescent pH indicators
US5622821A (en) * 1994-06-29 1997-04-22 The Regents Of The University Of California Luminescent lanthanide chelates and methods of use
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
US5863727A (en) * 1996-05-03 1999-01-26 The Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
JP3200024B2 (ja) * 1997-02-19 2001-08-20 哲雄 長野 ジアミノフルオレセイン誘導体
US6037137A (en) * 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
DE69817186T2 (de) * 1997-07-02 2004-05-27 Nagano, Testuo Diaminorhodamin-derivate
FR2768817B1 (fr) * 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique
EP1069121B1 (de) * 1998-03-31 2003-07-16 Nagano, Testuo Reagenz zum nachweis von singulett-sauerstoff
AU4962899A (en) * 1998-06-29 2000-01-17 Fluorrx, Inc. Assay method and device
ATE279411T1 (de) * 1998-08-12 2004-10-15 Daiichi Pure Chemicals Co Ltd Fluorszierende marker
JP2000111480A (ja) * 1998-10-06 2000-04-21 Kazuko Matsumoto 新規標識試薬
US6972182B1 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using coiled binding partners
JP4226245B2 (ja) * 1999-06-02 2009-02-18 哲雄 長野 Ｉｐ３受容体リガンド
US20020177120A1 (en) * 1999-06-04 2002-11-28 Kathryn J. Elliott Assays for apotosis modulators
US6441197B1 (en) * 2000-01-20 2002-08-27 Daiichi Pure Chemicals Co., Ltd. Diaminofluorescein derivative
KR20030010585A (ko) * 2000-02-28 2003-02-05 다이이치 가가쿠 야쿠힝 가부시키가이샤 아연 형광탐침
US6756231B1 (en) * 2000-08-18 2004-06-29 Daiichi Pure Chemicals Co., Ltd. Diaminorhodamine derivative

Also Published As

Publication number Publication date
KR20030011777A (ko) 2003-02-11
CN1222767C (zh) 2005-10-12
AU3599601A (en) 2001-09-03
DE60134219D1 (de) 2008-07-10
EP1271133A4 (de) 2006-02-08
JP4589588B2 (ja) 2010-12-01
EP1271133A1 (de) 2003-01-02
WO2001063265A1 (fr) 2001-08-30
US20030157727A1 (en) 2003-08-21
CA2401360A1 (en) 2001-08-30
CN1416527A (zh) 2003-05-07
EP1271133B1 (de) 2008-05-28
US20060252099A1 (en) 2006-11-09
AU2001235996B2 (en) 2004-12-02

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