EP0929810A2 - Homogenes verfahren zur bestimmung von energieübertragung bei lumineszenz-assays - Google Patents
Homogenes verfahren zur bestimmung von energieübertragung bei lumineszenz-assaysInfo
- Publication number
- EP0929810A2 EP0929810A2 EP97919087A EP97919087A EP0929810A2 EP 0929810 A2 EP0929810 A2 EP 0929810A2 EP 97919087 A EP97919087 A EP 97919087A EP 97919087 A EP97919087 A EP 97919087A EP 0929810 A2 EP0929810 A2 EP 0929810A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- acceptor
- donor
- energy transfer
- assay
- label
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57434—Specifically defined cancers of prostate
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
- G01N33/6869—Interleukin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/743—Steroid hormones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/40—Rare earth chelates
Definitions
- temporal discrimination time-resolution
- organic donor-acceptor pairs of different decay times using pyrene as the long excited state donor, pulsed laser for excitation and phycoerythrin as the short decay-time acceptor (US 4,822,733).
- time-resolved energy transfer principle is applied for homogeneous solution hybridization using fluorescein (Morrison et al (1989) Illrd International Symposium on Quantitative Luminescence Spectrometry in Biomedical Sciences, Ghent Belgium) as acceptor.
- a particularly preferred group of long excited state lifetime lanthanide chelates are Compounds 1 to 12 the structure of which is dislosed in Scheme 1 to 5.
- ⁇ exc means excitation wavelength, ⁇ decay time and G x ⁇ luminescence yield.
- the values given in the schemes are measured from the compound coupled to a protein. 98/15830
- covalently conjugated probes e.g. those of xanthene dyes (rhodamine, tetramethylrhodamine, Texas
- the great number of acceptor molecules may compensate the long distance after bead coating, and the luminescent bead actually provide a energy accepting surface.
- the bead can absorb most of the donor emitted light, a simple radiative enery transfer can be applied, in which the energy transfer is a function of space angle and critical distance with 10 ⁇ m beads is in the range of micrometers.
- the binding proteins e.g. agglutinin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Endocrinology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Reproductive Health (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FI963989 | 1996-10-04 | ||
FI963989A FI963989A (fi) | 1996-10-04 | 1996-10-04 | Homogeenisiä määritysmenetelmiä, jotka perustuvat luminesenssienergiasiirtoon |
PCT/FI1997/000560 WO1998015830A2 (en) | 1996-10-04 | 1997-09-19 | Homogenous luminescence energy transfer assays |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0929810A2 true EP0929810A2 (de) | 1999-07-21 |
Family
ID=8546810
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP97919087A Withdrawn EP0929810A2 (de) | 1996-10-04 | 1997-09-19 | Homogenes verfahren zur bestimmung von energieübertragung bei lumineszenz-assays |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0929810A2 (de) |
JP (1) | JP2001502055A (de) |
FI (1) | FI963989A (de) |
WO (1) | WO1998015830A2 (de) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1933151A1 (de) | 2006-12-15 | 2008-06-18 | F. Hoffmann-La Roche Ag | Prüfungen zum Messen der Phosphatmodifikationsenzymaktivität |
Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5998146A (en) * | 1998-07-17 | 1999-12-07 | Wallac Oy | Homogeneous luminescence assay method based on energy transfer |
EP1141404A1 (de) * | 1998-12-24 | 2001-10-10 | Aclara BioSciences, Inc. | Individuell adressierbare feste oberflächen, geeignet für parallele analysen |
FR2792072A1 (fr) * | 1999-04-08 | 2000-10-13 | Cis Bio Int | Methode homogene de detection ou de determination d'une interaction chimique ou physicochimique |
WO2001040805A1 (en) * | 1999-11-30 | 2001-06-07 | Akzo Nobel N.V. | Steroid compounds for steroid receptor binding assays |
CA2333346C (en) * | 2000-01-31 | 2013-01-22 | The Penn State Research Foundation | Interrogation of changes in the contents of a sealed container |
FI20000333A0 (fi) | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
US7160735B2 (en) | 2000-04-28 | 2007-01-09 | Monogram Biosciences, Inc. | Tagged microparticle compositions and methods |
US7771929B2 (en) | 2000-04-28 | 2010-08-10 | Monogram Biosciences, Inc. | Tag library compounds, compositions, kits and methods of use |
FR2809817B1 (fr) * | 2000-06-02 | 2003-08-15 | Cis Bio Int | Procede de detection de presence d'un liquide dans un melange |
DE10058596A1 (de) * | 2000-11-25 | 2002-06-06 | Aventis Pharma Gmbh | Verfahren zum Screening von chemischen Verbindungen zur Modulierung der Wechselwirkung einer EVH1-Domäne oder eines Proteins mit einer EVH1-Domäne mit einer EVH1-Bindedomäne oder einem Protein mit einer EVH1-Bindedomäne sowie ein Verfahren zum Nachweis besagter Wechselwirkung |
WO2002088733A1 (fr) | 2001-04-23 | 2002-11-07 | Eiken Kagaku Kabushiki Kaisha | Methode d'analyse faisant intervenir une interaction intermoleculaire par rapporteur (marqueur) |
AU2002305658A1 (en) | 2001-05-21 | 2002-12-03 | Aclara Biosciences, Inc. | Analyzing phosphorylated proteins |
AU2002310012A1 (en) | 2001-05-21 | 2002-12-03 | Monogram Biosciences, Inc. | Methods and compositions for analyzing proteins |
WO2002097112A2 (en) | 2001-05-26 | 2002-12-05 | Aclara Biosciences, Inc. | Catalytic amplification of multiplexed assay signals |
US7211440B2 (en) * | 2002-03-08 | 2007-05-01 | Wallac Oy | Dissociative fluorescence enhancement assay |
US20040229294A1 (en) | 2002-05-21 | 2004-11-18 | Po-Ying Chan-Hui | ErbB surface receptor complexes as biomarkers |
JP4206378B2 (ja) | 2002-07-08 | 2009-01-07 | 哲雄 長野 | 蛍光プローブ |
EP1608980B1 (de) * | 2003-03-28 | 2007-08-15 | SOUKKA, Tero | Bioassay mit energietransfer mit homogener lumineszenz |
US7696245B2 (en) | 2003-03-28 | 2010-04-13 | Sekisui Medical Co., Ltd. | Fluorescent probe for zinc |
FI20030460A0 (fi) | 2003-03-28 | 2003-03-28 | Tero Soukka | Homogeeninen määritysmenetelmä, joka perustuu luminesenssienergiasiirtoon |
EP1673634B1 (de) * | 2003-10-01 | 2008-08-06 | Wallac Oy | Verbesserter homogenener zeitaufgelöster energietransfertest |
WO2005062048A1 (de) * | 2003-12-01 | 2005-07-07 | Dade Behring Marburg Gmbh | Homogenes nachweisverfahren |
JP2005194244A (ja) | 2004-01-09 | 2005-07-21 | Shigenobu Yano | 亜鉛イオン蛍光センサー |
DE112005002567T5 (de) * | 2004-10-19 | 2007-11-22 | Wallac Oy | Eine neue Sonde und deren Verwendung in Bioaffinitäts-Analysen |
DE112005002636T5 (de) * | 2004-10-22 | 2007-09-06 | Wallac Oy | Hochempfindliche homogene Analyse auf der Grundlage einer FRET-Messung mit Anti-Stokes-Verschiebung |
JP4683572B2 (ja) * | 2005-01-28 | 2011-05-18 | 塩野義製薬株式会社 | 組換え蛋白質の定量法 |
WO2006125855A1 (en) * | 2005-05-24 | 2006-11-30 | Hidex Oy | Correction method and measurement device for anti-stokes photoluminescence measurement |
WO2007077291A1 (en) | 2005-12-30 | 2007-07-12 | Turun Yliopisto | Method for determination of concentration, charge or unit size of a substance |
WO2007125822A1 (ja) * | 2006-04-28 | 2007-11-08 | Tokyo Institute Of Technology | 薬剤スクリーニングのための複合体 |
EP2053059A1 (de) | 2007-10-26 | 2009-04-29 | Sanofi-Aventis | Komplexe von TRPC-Domänen und SESTD1 Domänen und Verfahren und Verwendungen damit |
US8652785B2 (en) | 2008-12-04 | 2014-02-18 | Sanofi | Method of screening a modulator of endothelial NO synthase comprising the use of heme binding protein 1 |
EP2226633A1 (de) | 2009-03-06 | 2010-09-08 | Charité - Universitätsmedizin Berlin | Verwendungen und Verfahren zur Identifikation einer zytoprotektiven Verbindung mit gp130 oder LIFRalpha |
JP5732452B2 (ja) * | 2009-04-30 | 2015-06-10 | セイエス ビオ アンテルナショナル | Vftドメイン膜タンパク質のダイマーを調節する化合物の検出方法 |
EP2267152A1 (de) | 2009-06-26 | 2010-12-29 | Sanofi-Aventis | Testsystem zum Messen der MEST-Aktivität, sowie Verfahren und Verwendungen damit |
EP2320236A1 (de) | 2009-11-06 | 2011-05-11 | Sanofi-aventis | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
EP2348130A1 (de) | 2010-01-21 | 2011-07-27 | Sanofi | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
EP2348128A1 (de) | 2010-01-21 | 2011-07-27 | Sanofi | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
EP2348129A1 (de) | 2010-01-21 | 2011-07-27 | Sanofi | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
WO2011113888A1 (en) | 2010-03-18 | 2011-09-22 | Sanofi-Aventis | Methods and uses relating to the identification of compound involved in pain as well as methods of diagnosing algesia |
US20130079238A1 (en) | 2010-03-18 | 2013-03-28 | Sanofi | Methods and uses relating to the identification of compound involved in pain as well as methods of diagnosing algesia |
WO2011113890A1 (en) | 2010-03-18 | 2011-09-22 | Sanofi | Methods and uses relating to the identification of compound involved in pain as well as methods of diagnosing algesia |
EP2390348A1 (de) | 2010-05-25 | 2011-11-30 | Sanofi | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
EP2390349A1 (de) | 2010-05-25 | 2011-11-30 | Sanofi | Verfahren und Verwendungen im Zusammenhang mit der Identifizierung einer am Schmerz beteiligten Verbindung sowie Verfahren zur Diagnose von Algesie |
EP2532755A1 (de) | 2011-06-10 | 2012-12-12 | Sanofi-Aventis | Verfahren und Verwendungen auf Grundlage der Slfn2 Expression zur Identifizierung und Profilierung von Verbindungen zur Verwendung bei der Behandlung oder Vorbeugung von Schmerzen |
CN102353790B (zh) * | 2011-06-25 | 2014-03-05 | 南方医科大学 | 一种甲胎蛋白的均相时间分辨荧光分析方法 |
EP2864783A4 (de) | 2012-06-22 | 2016-02-10 | Univ Macquarie | Multiplex-suspensionsassay/-array mittels lebensdauercodierung |
BR112015025832B1 (pt) | 2013-04-15 | 2022-03-15 | Wallac Oy | Método e dispositivo para correção de diafonia e instrumento de medida |
CN104181310A (zh) * | 2014-07-30 | 2014-12-03 | 美艾利尔(上海)诊断产品有限公司 | 一种检测β-hCG含量的时间分辨免疫荧光试剂盒及其制备方法和应用 |
SG11202002065VA (en) | 2017-09-13 | 2020-04-29 | Progenity Inc | Preeclampsia biomarkers and related systems and methods |
EP4070113A4 (de) | 2019-12-04 | 2023-12-20 | Biora Therapeutics, Inc. | Beurteilung von präeklampsie unter verwendung von tests für freien und dissoziierten plazentalen wachstumsfaktor |
FI130554B (en) | 2020-01-20 | 2023-11-16 | Oy Arctic Partners Ab | LUMINESCENCE HYBRIDIZATION DETERMINATION METHOD |
Family Cites Families (12)
Publication number | Priority date | Publication date | Assignee | Title |
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US4996143A (en) * | 1985-12-23 | 1991-02-26 | Syngene, Inc. | Fluorescent stokes shift probes for polynucleotide hybridization |
US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
EP0272320B1 (de) * | 1986-06-17 | 1994-03-23 | Baxter Diagnostics Inc. | Homogenes fluortestverfahren mit abstoss des fluorzenten hintergrundes. |
US5194300A (en) * | 1987-07-15 | 1993-03-16 | Cheung Sau W | Methods of making fluorescent microspheres |
GB8820887D0 (en) * | 1988-09-06 | 1988-10-05 | Atomic Energy Authority Uk | Detecting selected species |
US5326692B1 (en) * | 1992-05-13 | 1996-04-30 | Molecular Probes Inc | Fluorescent microparticles with controllable enhanced stokes shift |
FR2664699B1 (fr) * | 1990-07-13 | 1995-08-18 | Cis Bio Int | Procede d'amplification du signal d'emission d'un compose luminescent. |
FR2664702B1 (fr) * | 1990-07-13 | 1993-08-06 | Cis Bio Int | Procede de reduction d'interferences dans un dosage par fluorescence. |
US5457186A (en) * | 1993-10-13 | 1995-10-10 | Wallac Oy | Luminescent lanthanide chelates with decreased non-radiative energy loss |
US5622821A (en) * | 1994-06-29 | 1997-04-22 | The Regents Of The University Of California | Luminescent lanthanide chelates and methods of use |
FI96367C (fi) * | 1994-10-17 | 1996-06-10 | Erkki Juhani Soini | Biospecifinen määritysmenetelmä |
US5741657A (en) * | 1995-03-20 | 1998-04-21 | The Regents Of The University Of California | Fluorogenic substrates for β-lactamase and methods of use |
-
1996
- 1996-10-04 FI FI963989A patent/FI963989A/fi unknown
-
1997
- 1997-09-19 EP EP97919087A patent/EP0929810A2/de not_active Withdrawn
- 1997-09-19 JP JP10517208A patent/JP2001502055A/ja active Pending
- 1997-09-19 WO PCT/FI1997/000560 patent/WO1998015830A2/en not_active Application Discontinuation
Non-Patent Citations (1)
Title |
---|
See references of WO9815830A2 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1933151A1 (de) | 2006-12-15 | 2008-06-18 | F. Hoffmann-La Roche Ag | Prüfungen zum Messen der Phosphatmodifikationsenzymaktivität |
Also Published As
Publication number | Publication date |
---|---|
WO1998015830A3 (en) | 1998-06-18 |
FI963989A0 (fi) | 1996-10-04 |
JP2001502055A (ja) | 2001-02-13 |
WO1998015830A2 (en) | 1998-04-16 |
FI963989A (fi) | 1998-04-05 |
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