ATE295535T1 - Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung - Google Patents

Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Info

Publication number
ATE295535T1
ATE295535T1 AT01920851T AT01920851T ATE295535T1 AT E295535 T1 ATE295535 T1 AT E295535T1 AT 01920851 T AT01920851 T AT 01920851T AT 01920851 T AT01920851 T AT 01920851T AT E295535 T1 ATE295535 T1 AT E295535T1
Authority
AT
Austria
Prior art keywords
solution
charged molecules
analyzing charged
isoelectrical focusing
isoelectrical
Prior art date
Application number
AT01920851T
Other languages
English (en)
Inventor
David W Speicher
Xun Zuo
Original Assignee
Wistar Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wistar Inst filed Critical Wistar Inst
Application granted granted Critical
Publication of ATE295535T1 publication Critical patent/ATE295535T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
AT01920851T 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung ATE295535T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/541,520 US6638408B1 (en) 2000-04-03 2000-04-03 Method and device for separation of charged molecules by solution isoelectric focusing
PCT/US2001/010012 WO2001075432A2 (en) 2000-04-03 2001-03-29 A method and device for analysis of charged molecules by solution isoelectric focusing

Publications (1)

Publication Number Publication Date
ATE295535T1 true ATE295535T1 (de) 2005-05-15

Family

ID=24159922

Family Applications (2)

Application Number Title Priority Date Filing Date
AT01920851T ATE295535T1 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung
AT05010119T ATE485509T1 (de) 2000-04-03 2001-03-29 Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT05010119T ATE485509T1 (de) 2000-04-03 2001-03-29 Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung

Country Status (8)

Country Link
US (3) US6638408B1 (de)
EP (2) EP1269178B1 (de)
JP (1) JP4682348B2 (de)
AT (2) ATE295535T1 (de)
AU (2) AU4786101A (de)
CA (1) CA2405501C (de)
DE (2) DE60110769T2 (de)
WO (1) WO2001075432A2 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001036449A1 (en) * 1999-11-15 2001-05-25 Proteome Systems Ltd Multi-compartment electrophoresis
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
CN1235667C (zh) * 2000-10-06 2006-01-11 格拉迪普有限公司 多口分离装置及方法
AUPR161400A0 (en) * 2000-11-22 2000-12-14 Life Therapeutics Limited Apparatus and method for isoelectric focusing separations
JP4108037B2 (ja) * 2001-07-16 2008-06-25 プロテイン， フォレスト， インコーポレイテッド 生体分子の等電点によって生体分子を分析するためのバッファのアレイ
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US20030164297A1 (en) * 2002-03-04 2003-09-04 Corning Incorporated Electrophoretic inorganic porous material
SE0201655D0 (sv) * 2002-05-31 2002-05-31 Amersham Biosciences Ab A method of electrophoresis
GB0212853D0 (en) * 2002-06-01 2002-07-17 Novartis Pharma Ag Separation of molecules
US20030226752A1 (en) * 2002-06-05 2003-12-11 Gradipore Limited Method for pH-biased isoelectric trapping separation
EP1540324A2 (de) * 2002-06-07 2005-06-15 Picosep A/S Verfahren und system zum mehrstufigen isoelektrischen fokussieren
ATE525643T1 (de) * 2002-06-07 2011-10-15 Picosep As Verfahren und system zum mehrstufigen isoelektrischen fokussieren
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
EP1668350B1 (de) * 2003-09-24 2012-06-13 Agilent Technologies, Inc. Elektroforetische trennung von amphoterischen molekülen
WO2005029061A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Extraction of molecules using frame
ATE424449T1 (de) * 2003-10-07 2009-03-15 Univ Newcastle Res Ass Trennung von spermazellen durch elektrophorese
CA2536041A1 (en) * 2003-11-10 2005-05-26 Angiotech International Ag Medical implants and fibrosis-inducing agents
US20050161332A1 (en) * 2004-01-23 2005-07-28 Gyula Vigh Method and apparatus to improve the concentration detection sensitivity in isoelectric focusing systems
FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
US7704360B2 (en) * 2005-03-14 2010-04-27 Agilent Technologies, Inc. Devices and methods for separating sample constituents
US8809773B2 (en) * 2005-03-22 2014-08-19 Carnegie Mellon University Membrane interface apparatus and method for mass spectrometry
DE102005020135A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung der kontinuierlichen Ablenkungselektrophorese mit Hilfe von mehreren Gegenstrommedien
KR20070054053A (ko) * 2005-11-22 2007-05-28 삼성전자주식회사 전기영동표시장치와 전기영동표시장치의 구동방법
EP1968997B1 (de) * 2005-12-22 2016-06-29 GE Healthcare Bio-Sciences AB Gel für die isoelektrische fokussierung
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
WO2007126354A1 (en) * 2006-04-27 2007-11-08 Ge Healthcare Bio-Sciences Ab Electrodic bridge
US20090178929A1 (en) * 2006-06-02 2009-07-16 Koninklijke Philips Electronics N.V. Separation device for isoelectric focusing
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
US8988881B2 (en) 2007-12-18 2015-03-24 Sandia Corporation Heat exchanger device and method for heat removal or transfer
EP2146200A1 (de) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
US9005417B1 (en) 2008-10-01 2015-04-14 Sandia Corporation Devices, systems, and methods for microscale isoelectric fractionation
WO2010111265A1 (en) 2009-03-24 2010-09-30 University Of Chicago Slip chip device and methods
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US8945914B1 (en) 2010-07-08 2015-02-03 Sandia Corporation Devices, systems, and methods for conducting sandwich assays using sedimentation
US9795961B1 (en) 2010-07-08 2017-10-24 National Technology & Engineering Solutions Of Sandia, Llc Devices, systems, and methods for detecting nucleic acids using sedimentation
US8962346B2 (en) 2010-07-08 2015-02-24 Sandia Corporation Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
US9261100B2 (en) 2010-08-13 2016-02-16 Sandia Corporation Axial flow heat exchanger devices and methods for heat transfer using axial flow devices
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
EP2773743B1 (de) * 2011-11-04 2019-05-22 Bio-Rad Laboratories, Inc. Affinitätsverfahren und -zusammensetzungen mit elektronischer ph-steuerung
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US9182372B2 (en) 2011-11-22 2015-11-10 Stephen G. Haralampu Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
EP2814597A4 (de) * 2012-02-15 2015-10-28 Bio Rad Laboratories Elektronische steuerung von ph-wert und ionenstärke
US9244065B1 (en) 2012-03-16 2016-01-26 Sandia Corporation Systems, devices, and methods for agglutination assays using sedimentation
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US20140051104A1 (en) * 2012-08-15 2014-02-20 Array Bridge Inc. Methods for protein purification and analysis
JP2014059160A (ja) * 2012-09-14 2014-04-03 Sharp Corp 電気泳動用試験具およびその製造方法
US20160252516A1 (en) * 2013-10-03 2016-09-01 Northwestern University System and method for high throughput mass spectrometric analysis of proteome samples
US10533967B2 (en) * 2015-04-10 2020-01-14 Panasonic Intellectual Property Management Co., Ltd. Electrophoretic support body and electrophoretic device
KR20210142668A (ko) * 2019-03-14 2021-11-25 해이스택 사이언시스 코포레이션 올리고뉴클레오티드 코딩된 분자를 프로세싱 또는 분석하기 위한 방법 및 시스템

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
GB2036081B (en) * 1977-06-15 1982-08-25 Nat Res Dev Membrane electrophoresis
US4204929A (en) * 1978-04-18 1980-05-27 University Patents, Inc. Isoelectric focusing method
DE3337669C2 (de) * 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
US4673483A (en) * 1986-03-20 1987-06-16 Ionics Incorporated Isoelectric focusing apparatus
ATE107534T1 (de) * 1987-04-03 1994-07-15 Gradipore Ltd Trennung von geladenen molekülen.
DE3876273T2 (de) 1987-04-11 1993-05-27 Ciba Geigy Ag Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens.
US4824547A (en) * 1987-09-04 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Electrophoretic extraction of proteins from two-dimensional electrophoresis gel spots
US4994165A (en) * 1989-02-16 1991-02-19 Cornell Research Foundation, Inc. Liquid junction coupling for capillary zone electrophoresis/ion spray spectrometry
US4963236A (en) * 1989-03-08 1990-10-16 Ampholife Technologies Apparatus and methods for isoelectric focusing
US5160594A (en) * 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
US5336387A (en) * 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
ZA981370B (en) * 1997-02-20 1998-09-07 Cerberus Developments Bv Method and apparatus for continuous flow isoelectric focusing for purifying biological substances
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6171466B1 (en) * 1999-03-29 2001-01-09 Percy H. Rhodes Method and apparatus for electrophoretic focusing

Also Published As

Publication number Publication date
US20030205471A1 (en) 2003-11-06
CA2405501A1 (en) 2001-10-11
EP1269178A2 (de) 2003-01-02
US6638408B1 (en) 2003-10-28
EP1564550B1 (de) 2010-10-20
EP1564550A2 (de) 2005-08-17
EP1564550A3 (de) 2005-08-24
CA2405501C (en) 2007-11-13
AU2001247861B2 (en) 2006-06-08
DE60143325D1 (de) 2010-12-02
JP2003529762A (ja) 2003-10-07
US20050087445A1 (en) 2005-04-28
DE60110769D1 (de) 2005-06-16
EP1269178B1 (de) 2005-05-11
WO2001075432A3 (en) 2002-06-06
AU4786101A (en) 2001-10-15
DE60110769T2 (de) 2006-02-23
WO2001075432A2 (en) 2001-10-11
JP4682348B2 (ja) 2011-05-11
ATE485509T1 (de) 2010-11-15

Similar Documents

Publication Publication Date Title
ATE295535T1 (de) Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung
DE69511229D1 (de) Verfahren zur Analyse von gleichmässig erregten mechanischen Schwingungen
DE3382384D1 (de) Verfahren und vorrichtung zur quantitativen analyse.
DE69609922D1 (de) Verfahren zur Trocknung von Gasen mittels Glycol mit anschliessender Raffinierung von gasförmigen Abflüssen
DE68907407D1 (de) Verfahren zur sequenzanalyse von dna.
DE69721585D1 (de) Verfahren zur aufnahme von flüssigen proben für analytische tests
DE60204669D1 (de) Verfahren und vorrichtung zur gel-freien qualitativen proteomanalyse und deren verwendungen
DE69836648D1 (de) Vorrichtung und Verfahren zur Trennung von Flüssigkeitsbestandteilen
ATE338951T1 (de) Vorrichtung zur automatischen durchführung von laboratoriumprüfungen
DE69712433D1 (de) Verfahren und Vorrichtung zur Gewinnung von Lithium durch Anlegen eines Spannung über einem Lithion-Ionen leitenden Festelektrolyt
DE69602811D1 (de) Vorrichtung zur Herstellung von Musterproben
DE69905425D1 (de) Verfahren und vorrichtung zur trennung von bioorganischen molekülen mittels elektrophorese
ATE217962T1 (de) Vorrichtung zur parallel-ausrichtung von makromolekülen und verwendung davon
ATE108904T1 (de) Verfahren zur untersuchung von relaxationsspektren und resonanzen in materialien.
DE3752148D1 (de) Echtzeitabtastvorrichtung in einem Elektrophoreseapparat zur DNS-Sequenzbestimmung
DE69420750D1 (de) Verfahren zur Probenanalyse mittels Kapillarelektroforese
ATE299589T1 (de) Verfahren zur bestimmung von substanzen mittels der evaneszenzfeldmethode
DE19581819T1 (de) Verfahren und Vorrichtung zur Herstellung von Substanzen für optische Analysen
ATE197507T1 (de) Verfahren und verbindungen zur detektion von analyten mittels remanenzmessung und deren verwendung
ATE455787T1 (de) Verfahren und vorrichtung zur abtrennung von biomolekülen
ATE449843T1 (de) Elektrophoretischer trennung von nukleinsäuren aus biologischen proben bei niedrigem ph-wert
DE69433382D1 (de) Vorrichtung zur wiederholten probenentnahme mittels beweglicher probenröhrchen
ATE213227T1 (de) Verfahren und vorrichtung zur aufreinigung und anreicherung von molekülen
DD139373A1 (de) Verfahren und vorrichtung zur identifikation chemischer analysenproben
DE69024899D1 (de) Verfahren und Einrichtung zur Analyse durch Synthetisieren von Sprache

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties