ATE485509T1 - Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung - Google Patents

Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung

Info

Publication number
ATE485509T1
ATE485509T1 AT05010119T AT05010119T ATE485509T1 AT E485509 T1 ATE485509 T1 AT E485509T1 AT 05010119 T AT05010119 T AT 05010119T AT 05010119 T AT05010119 T AT 05010119T AT E485509 T1 ATE485509 T1 AT E485509T1
Authority
AT
Austria
Prior art keywords
solution
analyzing proteins
proteins
isoelectrical focusing
isoelectrical
Prior art date
Application number
AT05010119T
Other languages
English (en)
Inventor
David W Speicher
Xun Zuo
Original Assignee
Wistar Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wistar Inst filed Critical Wistar Inst
Application granted granted Critical
Publication of ATE485509T1 publication Critical patent/ATE485509T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
AT05010119T 2000-04-03 2001-03-29 Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung ATE485509T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/541,520 US6638408B1 (en) 2000-04-03 2000-04-03 Method and device for separation of charged molecules by solution isoelectric focusing

Publications (1)

Publication Number Publication Date
ATE485509T1 true ATE485509T1 (de) 2010-11-15

Family

ID=24159922

Family Applications (2)

Application Number Title Priority Date Filing Date
AT05010119T ATE485509T1 (de) 2000-04-03 2001-03-29 Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung
AT01920851T ATE295535T1 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT01920851T ATE295535T1 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Country Status (8)

Country Link
US (3) US6638408B1 (de)
EP (2) EP1269178B1 (de)
JP (1) JP4682348B2 (de)
AT (2) ATE485509T1 (de)
AU (2) AU2001247861B2 (de)
CA (1) CA2405501C (de)
DE (2) DE60110769T2 (de)
WO (1) WO2001075432A2 (de)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003514829A (ja) * 1999-11-15 2003-04-22 プロテオム システムズ リミテッド 多室電気泳動
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
US20030019753A1 (en) * 2000-10-05 2003-01-30 David Ogle Multi-port separation apparatus and method
AUPR161400A0 (en) * 2000-11-22 2000-12-14 Life Therapeutics Limited Apparatus and method for isoelectric focusing separations
IL159810A0 (en) * 2001-07-16 2004-06-20 Protein Forest Inc Matrixes, arrays, systems and methods
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US20030164297A1 (en) * 2002-03-04 2003-09-04 Corning Incorporated Electrophoretic inorganic porous material
SE0201655D0 (sv) * 2002-05-31 2002-05-31 Amersham Biosciences Ab A method of electrophoresis
GB0212853D0 (en) * 2002-06-01 2002-07-17 Novartis Pharma Ag Separation of molecules
US20030226752A1 (en) * 2002-06-05 2003-12-11 Gradipore Limited Method for pH-biased isoelectric trapping separation
WO2003104792A2 (en) * 2002-06-07 2003-12-18 Picosep A/S A method of separating biocomponents contained in a liquid, a separating system and a separating unit
ATE525643T1 (de) * 2002-06-07 2011-10-15 Picosep As Verfahren und system zum mehrstufigen isoelektrischen fokussieren
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
WO2005029061A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Extraction of molecules using frame
WO2005029060A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
EP1689853B1 (de) * 2003-10-07 2009-03-04 The University Of Newcastle Research Associates Limited Trennung von spermazellen durch elektrophorese
EP1691852A2 (de) * 2003-11-10 2006-08-23 Angiotech International AG Medizinsche implantate und fibroseinduzierende mittel
US20050161332A1 (en) * 2004-01-23 2005-07-28 Gyula Vigh Method and apparatus to improve the concentration detection sensitivity in isoelectric focusing systems
FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
US7704360B2 (en) * 2005-03-14 2010-04-27 Agilent Technologies, Inc. Devices and methods for separating sample constituents
WO2006102520A2 (en) * 2005-03-22 2006-09-28 Carnegie Mellon University Membrane interface apparatus and method for analysis of volatile molecules by mass spectrometry
DE102005020135A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung der kontinuierlichen Ablenkungselektrophorese mit Hilfe von mehreren Gegenstrommedien
KR20070054053A (ko) * 2005-11-22 2007-05-28 삼성전자주식회사 전기영동표시장치와 전기영동표시장치의 구동방법
US20080289963A1 (en) * 2005-12-22 2008-11-27 Ge Healthcare Bio-Sciences Ab Gel for Isoelectric Focusing
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
AU2007243994B2 (en) * 2006-04-27 2012-08-02 Ge Healthcare Bio-Sciences Ab Electrodic bridge
US20090178929A1 (en) * 2006-06-02 2009-07-16 Koninklijke Philips Electronics N.V. Separation device for isoelectric focusing
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
US8988881B2 (en) 2007-12-18 2015-03-24 Sandia Corporation Heat exchanger device and method for heat removal or transfer
EP2146200A1 (de) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
US9005417B1 (en) 2008-10-01 2015-04-14 Sandia Corporation Devices, systems, and methods for microscale isoelectric fractionation
JP5766178B2 (ja) 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ ＳｌｉｐＣｈｉｐ装置および方法
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US8962346B2 (en) 2010-07-08 2015-02-24 Sandia Corporation Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
US8945914B1 (en) 2010-07-08 2015-02-03 Sandia Corporation Devices, systems, and methods for conducting sandwich assays using sedimentation
US9795961B1 (en) 2010-07-08 2017-10-24 National Technology & Engineering Solutions Of Sandia, Llc Devices, systems, and methods for detecting nucleic acids using sedimentation
US9261100B2 (en) 2010-08-13 2016-02-16 Sandia Corporation Axial flow heat exchanger devices and methods for heat transfer using axial flow devices
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
EP2773743B1 (de) * 2011-11-04 2019-05-22 Bio-Rad Laboratories, Inc. Affinitätsverfahren und -zusammensetzungen mit elektronischer ph-steuerung
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2788746B1 (de) 2011-11-22 2018-10-24 Stephen G. Haralampu Mikrofluidische vorrichtung mit gestopptem fluss sowie verfahren zur ladungsbasierten trennung komplexer analytmischungen
WO2013123425A1 (en) * 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9244065B1 (en) 2012-03-16 2016-01-26 Sandia Corporation Systems, devices, and methods for agglutination assays using sedimentation
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US20140051104A1 (en) * 2012-08-15 2014-02-20 Array Bridge Inc. Methods for protein purification and analysis
JP2014059160A (ja) * 2012-09-14 2014-04-03 Sharp Corp 電気泳動用試験具およびその製造方法
US20160252516A1 (en) * 2013-10-03 2016-09-01 Northwestern University System and method for high throughput mass spectrometric analysis of proteome samples
WO2016163097A1 (ja) * 2015-04-10 2016-10-13 パナソニックＩｐマネジメント株式会社 電気泳動支持体及び電気泳動装置
JP2022525340A (ja) * 2019-03-14 2022-05-12 ヘイスタック サイエンシーズ コーポレイション オリゴヌクレオチドコード化分子を加工または分析する方法および系

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
DE2861803D1 (en) * 1977-06-15 1982-07-01 Nat Res Dev Electrophoresis membranes, their use in a separation method and separation apparatus
US4204929A (en) * 1978-04-18 1980-05-27 University Patents, Inc. Isoelectric focusing method
DE3337669C2 (de) * 1983-10-17 1989-09-21 Carl Schleicher & Schuell Gmbh & Co Kg, 3352 Einbeck Gerät zur Elektroelution elektrisch geladener Makromoleküle
US4673483A (en) * 1986-03-20 1987-06-16 Ionics Incorporated Isoelectric focusing apparatus
ATE107534T1 (de) * 1987-04-03 1994-07-15 Gradipore Ltd Trennung von geladenen molekülen.
EP0287513B1 (de) * 1987-04-11 1992-12-02 Ciba-Geigy Ag Isoelektrisches Fokussierverfahren sowie Einrichtung zur Durchführung dieses Verfahrens
US4824547A (en) * 1987-09-04 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Electrophoretic extraction of proteins from two-dimensional electrophoresis gel spots
US4994165A (en) * 1989-02-16 1991-02-19 Cornell Research Foundation, Inc. Liquid junction coupling for capillary zone electrophoresis/ion spray spectrometry
US5160594A (en) * 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
US4963236A (en) * 1989-03-08 1990-10-16 Ampholife Technologies Apparatus and methods for isoelectric focusing
US5336387A (en) * 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
ZA981370B (en) * 1997-02-20 1998-09-07 Cerberus Developments Bv Method and apparatus for continuous flow isoelectric focusing for purifying biological substances
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6171466B1 (en) * 1999-03-29 2001-01-09 Percy H. Rhodes Method and apparatus for electrophoretic focusing

Also Published As

Publication number Publication date
EP1564550A2 (de) 2005-08-17
AU4786101A (en) 2001-10-15
JP2003529762A (ja) 2003-10-07
DE60143325D1 (de) 2010-12-02
AU2001247861B2 (en) 2006-06-08
DE60110769D1 (de) 2005-06-16
CA2405501C (en) 2007-11-13
US20030205471A1 (en) 2003-11-06
JP4682348B2 (ja) 2011-05-11
EP1269178B1 (de) 2005-05-11
WO2001075432A2 (en) 2001-10-11
US20050087445A1 (en) 2005-04-28
CA2405501A1 (en) 2001-10-11
EP1564550A3 (de) 2005-08-24
EP1564550B1 (de) 2010-10-20
US6638408B1 (en) 2003-10-28
WO2001075432A3 (en) 2002-06-06
DE60110769T2 (de) 2006-02-23
EP1269178A2 (de) 2003-01-02
ATE295535T1 (de) 2005-05-15

Similar Documents

Publication Publication Date Title
ATE485509T1 (de) Verfahren zur analyse von proteinen durch isoelektrische fokussierung einer lösung
DE69511229D1 (de) Verfahren zur Analyse von gleichmässig erregten mechanischen Schwingungen
DE69735295D1 (de) Verfahren zur Aufkonzentrierung von Antikörperlösungen
DE68907407D1 (de) Verfahren zur sequenzanalyse von dna.
DE69112994D1 (de) Verfahren zur interaktionalen selbstmodelierenden analyse von gemischen.
DE69721585D1 (de) Verfahren zur aufnahme von flüssigen proben für analytische tests
DE69609922D1 (de) Verfahren zur Trocknung von Gasen mittels Glycol mit anschliessender Raffinierung von gasförmigen Abflüssen
DK1712623T3 (da) Udvælgelse af proteiner ved anvendelse af RNA-proteinfusioner
DE69113960D1 (de) ATR-Objektiv und Verfahren zur Probenuntersuchung.
ATE108904T1 (de) Verfahren zur untersuchung von relaxationsspektren und resonanzen in materialien.
DE3752148D1 (de) Echtzeitabtastvorrichtung in einem Elektrophoreseapparat zur DNS-Sequenzbestimmung
DE3865963D1 (de) Elektrophoretisches gel hoher aufloesung sowie methode zur abscheidung von serumproteinen.
ATE220558T1 (de) Verbessertes verfahren zur stabilisierung von proteinen
DE69420750D1 (de) Verfahren zur Probenanalyse mittels Kapillarelektroforese
DE69729726D1 (de) Verfahren zur trennung von wirkstoffgemischen mit kapillaraffinity-gelelektrophorese
ATE299589T1 (de) Verfahren zur bestimmung von substanzen mittels der evaneszenzfeldmethode
ATE341633T1 (de) Verfahren zur spaltung von fusionproteinen
DE3482680D1 (de) Verfahren zur analyse von aminosaeuren.
DE69902796D1 (de) Verfahren zur reinigung von biomolekül- oder proteinkomplexen
DE69518506D1 (de) Verfahren zur strukturierung von leiterbahnen ohne unterätzung
DE69941655D1 (de) Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert
AT367769B (de) Verfahren zur reinigung von proteinen
DE69927691D1 (de) Verfahren zur eiweissanalyse
ATE455787T1 (de) Verfahren und vorrichtung zur abtrennung von biomolekülen
DE69319752D1 (de) Verfahren zur identifizierung von analyten

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties