DE60143325D1 - Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung - Google Patents

Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung

Info

Publication number
DE60143325D1
DE60143325D1 DE60143325T DE60143325T DE60143325D1 DE 60143325 D1 DE60143325 D1 DE 60143325D1 DE 60143325 T DE60143325 T DE 60143325T DE 60143325 T DE60143325 T DE 60143325T DE 60143325 D1 DE60143325 D1 DE 60143325D1
Authority
DE
Germany
Prior art keywords
solution
isoelectric focusing
analyzing proteins
proteins
analyzing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60143325T
Other languages
English (en)
Inventor
David W Speicher
Xun Zuo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wistar Institute of Anatomy and Biology
Original Assignee
Wistar Institute of Anatomy and Biology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wistar Institute of Anatomy and Biology filed Critical Wistar Institute of Anatomy and Biology
Application granted granted Critical
Publication of DE60143325D1 publication Critical patent/DE60143325D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
DE60143325T 2000-04-03 2001-03-29 Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung Expired - Lifetime DE60143325D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/541,520 US6638408B1 (en) 2000-04-03 2000-04-03 Method and device for separation of charged molecules by solution isoelectric focusing

Publications (1)

Publication Number Publication Date
DE60143325D1 true DE60143325D1 (de) 2010-12-02

Family

ID=24159922

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60143325T Expired - Lifetime DE60143325D1 (de) 2000-04-03 2001-03-29 Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung
DE60110769T Expired - Lifetime DE60110769T2 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE60110769T Expired - Lifetime DE60110769T2 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Country Status (8)

Country Link
US (3) US6638408B1 (de)
EP (2) EP1564550B1 (de)
JP (1) JP4682348B2 (de)
AT (2) ATE295535T1 (de)
AU (2) AU4786101A (de)
CA (1) CA2405501C (de)
DE (2) DE60143325D1 (de)
WO (1) WO2001075432A2 (de)

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JP4108037B2 (ja) * 2001-07-16 2008-06-25 プロテイン， フォレスト， インコーポレイテッド 生体分子の等電点によって生体分子を分析するためのバッファのアレイ
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AU2003234885A1 (en) * 2002-06-07 2003-12-22 Picosep A/S Method and system for multi-stage isoelectric focussing
EP1930721A3 (de) * 2002-06-07 2008-09-03 Picosep A/S Verfahren und System zum mehrstufigen Isoelektrischen fokussieren
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
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AU2004278448B2 (en) * 2003-10-07 2007-06-07 The University Of Newcastle Research Associates Limited Sperm cell separation by electrophoresis
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US20050161332A1 (en) * 2004-01-23 2005-07-28 Gyula Vigh Method and apparatus to improve the concentration detection sensitivity in isoelectric focusing systems
FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
US7704360B2 (en) * 2005-03-14 2010-04-27 Agilent Technologies, Inc. Devices and methods for separating sample constituents
WO2006102520A2 (en) * 2005-03-22 2006-09-28 Carnegie Mellon University Membrane interface apparatus and method for analysis of volatile molecules by mass spectrometry
DE102005020135A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung der kontinuierlichen Ablenkungselektrophorese mit Hilfe von mehreren Gegenstrommedien
KR20070054053A (ko) * 2005-11-22 2007-05-28 삼성전자주식회사 전기영동표시장치와 전기영동표시장치의 구동방법
EP1968997B1 (de) 2005-12-22 2016-06-29 GE Healthcare Bio-Sciences AB Gel für die isoelektrische fokussierung
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
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US20090178929A1 (en) * 2006-06-02 2009-07-16 Koninklijke Philips Electronics N.V. Separation device for isoelectric focusing
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
US8988881B2 (en) 2007-12-18 2015-03-24 Sandia Corporation Heat exchanger device and method for heat removal or transfer
EP2146200A1 (de) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
US9005417B1 (en) 2008-10-01 2015-04-14 Sandia Corporation Devices, systems, and methods for microscale isoelectric fractionation
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9415392B2 (en) 2009-03-24 2016-08-16 The University Of Chicago Slip chip device and methods
US9795961B1 (en) 2010-07-08 2017-10-24 National Technology & Engineering Solutions Of Sandia, Llc Devices, systems, and methods for detecting nucleic acids using sedimentation
US8945914B1 (en) 2010-07-08 2015-02-03 Sandia Corporation Devices, systems, and methods for conducting sandwich assays using sedimentation
US8962346B2 (en) 2010-07-08 2015-02-24 Sandia Corporation Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
US9261100B2 (en) 2010-08-13 2016-02-16 Sandia Corporation Axial flow heat exchanger devices and methods for heat transfer using axial flow devices
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
CN104024396A (zh) * 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 采用pH的电子控制的亲和方法及组合物
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CA2856779C (en) 2011-11-22 2021-07-20 Stephen G. Haralampu Stopped-flow, m icro-flu i imc device and method for the charge-based separation of complex analyte mixtures
EP2814597A4 (de) * 2012-02-15 2015-10-28 Bio Rad Laboratories Elektronische steuerung von ph-wert und ionenstärke
US9244065B1 (en) 2012-03-16 2016-01-26 Sandia Corporation Systems, devices, and methods for agglutination assays using sedimentation
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US20140051104A1 (en) * 2012-08-15 2014-02-20 Array Bridge Inc. Methods for protein purification and analysis
JP2014059160A (ja) * 2012-09-14 2014-04-03 Sharp Corp 電気泳動用試験具およびその製造方法
US20160252516A1 (en) * 2013-10-03 2016-09-01 Northwestern University System and method for high throughput mass spectrometric analysis of proteome samples
CN107430088B (zh) * 2015-04-10 2020-02-28 松下知识产权经营株式会社 电泳支承体和电泳装置
CN113677836A (zh) * 2019-03-14 2021-11-19 海斯塔克科学公司 用于处理或分析寡核苷酸编码分子的方法和系统

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US4824547A (en) * 1987-09-04 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Electrophoretic extraction of proteins from two-dimensional electrophoresis gel spots
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US6171466B1 (en) * 1999-03-29 2001-01-09 Percy H. Rhodes Method and apparatus for electrophoretic focusing

Also Published As

Publication number Publication date
ATE295535T1 (de) 2005-05-15
DE60110769D1 (de) 2005-06-16
US6638408B1 (en) 2003-10-28
CA2405501C (en) 2007-11-13
JP2003529762A (ja) 2003-10-07
US20030205471A1 (en) 2003-11-06
DE60110769T2 (de) 2006-02-23
WO2001075432A2 (en) 2001-10-11
AU4786101A (en) 2001-10-15
WO2001075432A3 (en) 2002-06-06
EP1564550A3 (de) 2005-08-24
ATE485509T1 (de) 2010-11-15
EP1269178B1 (de) 2005-05-11
EP1564550B1 (de) 2010-10-20
US20050087445A1 (en) 2005-04-28
CA2405501A1 (en) 2001-10-11
EP1269178A2 (de) 2003-01-02
AU2001247861B2 (en) 2006-06-08
JP4682348B2 (ja) 2011-05-11
EP1564550A2 (de) 2005-08-17

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