DE60110769D1 - Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung - Google Patents

Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung

Info

Publication number
DE60110769D1
DE60110769D1 DE60110769T DE60110769T DE60110769D1 DE 60110769 D1 DE60110769 D1 DE 60110769D1 DE 60110769 T DE60110769 T DE 60110769T DE 60110769 T DE60110769 T DE 60110769T DE 60110769 D1 DE60110769 D1 DE 60110769D1
Authority
DE
Germany
Prior art keywords
solution
analyzing
isoelectric focusing
loaded molecules
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60110769T
Other languages
English (en)
Other versions
DE60110769T2 (de
Inventor
W Speicher
Xun Zuo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wistar Institute of Anatomy and Biology
Original Assignee
Wistar Institute of Anatomy and Biology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wistar Institute of Anatomy and Biology filed Critical Wistar Institute of Anatomy and Biology
Publication of DE60110769D1 publication Critical patent/DE60110769D1/de
Application granted granted Critical
Publication of DE60110769T2 publication Critical patent/DE60110769T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
DE60110769T 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung Expired - Lifetime DE60110769T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/541,520 US6638408B1 (en) 2000-04-03 2000-04-03 Method and device for separation of charged molecules by solution isoelectric focusing
US541520 2000-04-03
PCT/US2001/010012 WO2001075432A2 (en) 2000-04-03 2001-03-29 A method and device for analysis of charged molecules by solution isoelectric focusing

Publications (2)

Publication Number Publication Date
DE60110769D1 true DE60110769D1 (de) 2005-06-16
DE60110769T2 DE60110769T2 (de) 2006-02-23

Family

ID=24159922

Family Applications (2)

Application Number Title Priority Date Filing Date
DE60110769T Expired - Lifetime DE60110769T2 (de) 2000-04-03 2001-03-29 Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung
DE60143325T Expired - Lifetime DE60143325D1 (de) 2000-04-03 2001-03-29 Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE60143325T Expired - Lifetime DE60143325D1 (de) 2000-04-03 2001-03-29 Verfahren zur Analyse von Proteinen durch isoelektrische Fokussierung einer Lösung

Country Status (8)

Country Link
US (3) US6638408B1 (de)
EP (2) EP1564550B1 (de)
JP (1) JP4682348B2 (de)
AT (2) ATE295535T1 (de)
AU (2) AU4786101A (de)
CA (1) CA2405501C (de)
DE (2) DE60110769T2 (de)
WO (1) WO2001075432A2 (de)

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AU2003234885A1 (en) * 2002-06-07 2003-12-22 Picosep A/S Method and system for multi-stage isoelectric focussing
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
AU2003278178A1 (en) * 2003-09-24 2005-04-11 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
WO2005029061A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Extraction of molecules using frame
AU2004278448B2 (en) * 2003-10-07 2007-06-07 The University Of Newcastle Research Associates Limited Sperm cell separation by electrophoresis
WO2005046746A2 (en) * 2003-11-10 2005-05-26 Angiotech International Ag Medical implants and fibrosis-inducing agents
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FR2867695B1 (fr) 2004-03-18 2006-12-29 Portmann Instr Dispositif et procede de filtration et/ou de separation de molecules
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
US7704360B2 (en) * 2005-03-14 2010-04-27 Agilent Technologies, Inc. Devices and methods for separating sample constituents
US8809773B2 (en) * 2005-03-22 2014-08-19 Carnegie Mellon University Membrane interface apparatus and method for mass spectrometry
DE102005020135A1 (de) * 2005-04-29 2006-11-02 Becton, Dickinson And Co. Verfahren und Vorrichtung zur Durchführung der kontinuierlichen Ablenkungselektrophorese mit Hilfe von mehreren Gegenstrommedien
KR20070054053A (ko) * 2005-11-22 2007-05-28 삼성전자주식회사 전기영동표시장치와 전기영동표시장치의 구동방법
WO2007073293A1 (en) * 2005-12-22 2007-06-28 Ge Healthcare Bio-Sciences Ab Gel for isoelectric focusing
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
US7964075B2 (en) 2006-04-27 2011-06-21 Ge Healthcare Bio-Sciences Ab Electrodic bridge
EP2030009A1 (de) * 2006-06-02 2009-03-04 Koninklijke Philips Electronics N.V. Trennvorrichtung für die isoelektrische fokussierung
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
US8988881B2 (en) 2007-12-18 2015-03-24 Sandia Corporation Heat exchanger device and method for heat removal or transfer
EP2146200A1 (de) 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
US9005417B1 (en) 2008-10-01 2015-04-14 Sandia Corporation Devices, systems, and methods for microscale isoelectric fractionation
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
KR101796906B1 (ko) 2009-03-24 2017-11-10 유니버시티 오브 시카고 반응을 수행하기 위한 방법
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US8945914B1 (en) 2010-07-08 2015-02-03 Sandia Corporation Devices, systems, and methods for conducting sandwich assays using sedimentation
US9795961B1 (en) 2010-07-08 2017-10-24 National Technology & Engineering Solutions Of Sandia, Llc Devices, systems, and methods for detecting nucleic acids using sedimentation
US8962346B2 (en) 2010-07-08 2015-02-24 Sandia Corporation Devices, systems, and methods for conducting assays with improved sensitivity using sedimentation
WO2012118982A2 (en) 2011-03-02 2012-09-07 Sandia Corporation Axial flow heat exchanger devices and methods for heat transfer using axial flow devices
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
US20130164860A1 (en) * 2011-11-04 2013-06-27 Technion Research & Development Foundation Ltd. Affinity methods and compositions employing electronic control of ph
EP2773221B1 (de) 2011-11-04 2019-01-09 Bio-Rad Laboratories, Inc. Gleichzeitige reinigung von zellkomponenten
CA2856779C (en) 2011-11-22 2021-07-20 Stephen G. Haralampu Stopped-flow, m icro-flu i imc device and method for the charge-based separation of complex analyte mixtures
US9658195B2 (en) * 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
US9244065B1 (en) 2012-03-16 2016-01-26 Sandia Corporation Systems, devices, and methods for agglutination assays using sedimentation
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US20140051104A1 (en) * 2012-08-15 2014-02-20 Array Bridge Inc. Methods for protein purification and analysis
JP2014059160A (ja) * 2012-09-14 2014-04-03 Sharp Corp 電気泳動用試験具およびその製造方法
US20160252516A1 (en) * 2013-10-03 2016-09-01 Northwestern University System and method for high throughput mass spectrometric analysis of proteome samples
WO2016163097A1 (ja) * 2015-04-10 2016-10-13 パナソニックＩｐマネジメント株式会社 電気泳動支持体及び電気泳動装置
KR20210142668A (ko) * 2019-03-14 2021-11-25 해이스택 사이언시스 코포레이션 올리고뉴클레오티드 코딩된 분자를 프로세싱 또는 분석하기 위한 방법 및 시스템

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US4824547A (en) * 1987-09-04 1989-04-25 The United States Of America As Represented By The United States Department Of Energy Electrophoretic extraction of proteins from two-dimensional electrophoresis gel spots
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Also Published As

Publication number Publication date
EP1564550B1 (de) 2010-10-20
CA2405501C (en) 2007-11-13
EP1269178A2 (de) 2003-01-02
US6638408B1 (en) 2003-10-28
US20030205471A1 (en) 2003-11-06
ATE485509T1 (de) 2010-11-15
WO2001075432A2 (en) 2001-10-11
WO2001075432A3 (en) 2002-06-06
JP4682348B2 (ja) 2011-05-11
AU2001247861B2 (en) 2006-06-08
US20050087445A1 (en) 2005-04-28
CA2405501A1 (en) 2001-10-11
EP1564550A2 (de) 2005-08-17
ATE295535T1 (de) 2005-05-15
DE60143325D1 (de) 2010-12-02
EP1269178B1 (de) 2005-05-11
JP2003529762A (ja) 2003-10-07
AU4786101A (en) 2001-10-15
DE60110769T2 (de) 2006-02-23
EP1564550A3 (de) 2005-08-24

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