ATE455787T1 - Verfahren und vorrichtung zur abtrennung von biomolekülen - Google Patents

Verfahren und vorrichtung zur abtrennung von biomolekülen

Info

Publication number
ATE455787T1
ATE455787T1 AT01976508T AT01976508T ATE455787T1 AT E455787 T1 ATE455787 T1 AT E455787T1 AT 01976508 T AT01976508 T AT 01976508T AT 01976508 T AT01976508 T AT 01976508T AT E455787 T1 ATE455787 T1 AT E455787T1
Authority
AT
Austria
Prior art keywords
molecules
focusing
section
separation
velocity
Prior art date
Application number
AT01976508T
Other languages
English (en)
Inventor
Giles Sanders
John Hassard
Stuart Hassard
Dimitrios Sideris
Original Assignee
Deltadot Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deltadot Ltd filed Critical Deltadot Ltd
Application granted granted Critical
Publication of ATE455787T1 publication Critical patent/ATE455787T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis
    • C07K1/28—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT01976508T 2000-12-15 2001-10-23 Verfahren und vorrichtung zur abtrennung von biomolekülen ATE455787T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0030708.2A GB0030708D0 (en) 2000-12-15 2000-12-15 Single channel proteomics concepts
PCT/GB2001/004696 WO2002048177A1 (en) 2000-12-15 2001-10-23 Method and apparatus for separating biological molecules

Publications (1)

Publication Number Publication Date
ATE455787T1 true ATE455787T1 (de) 2010-02-15

Family

ID=9905220

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01976508T ATE455787T1 (de) 2000-12-15 2001-10-23 Verfahren und vorrichtung zur abtrennung von biomolekülen

Country Status (12)

Country Link
US (1) US6979390B2 (de)
EP (1) EP1341806B1 (de)
JP (1) JP4054678B2 (de)
AT (1) ATE455787T1 (de)
AU (1) AU2001295776A1 (de)
CA (1) CA2431357A1 (de)
DE (1) DE60141159D1 (de)
DK (1) DK1341806T3 (de)
ES (1) ES2339744T3 (de)
GB (1) GB0030708D0 (de)
PT (1) PT1341806E (de)
WO (1) WO2002048177A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2489077A1 (en) * 2002-06-07 2003-12-18 Picosep A/S Method and system for multi-stage isoelectric focussing
GB0215779D0 (en) 2002-07-08 2002-08-14 Deltadot Ltd Material separation device
EP2127736A1 (de) 2003-04-10 2009-12-02 The President and Fellows of Harvard College Erzeugung und Kontrolle von Fluiden
CN1842368B (zh) 2003-08-27 2014-05-28 哈佛大学 流体物种的电子控制
JP2006038535A (ja) * 2004-07-23 2006-02-09 Sharp Corp 物質の検出方法および物質の分離装置
DE202005008763U1 (de) * 2005-06-02 2006-10-19 Universität Duisburg-Essen Vorrichtung zum Sortieren von Mikropartikeln, insbesondere biogefährdender und zu schützender Stoffe
GB0606181D0 (en) * 2006-03-29 2006-05-10 Deltadot Ltd Separation of biomolecules
WO2018182082A1 (ko) * 2017-03-27 2018-10-04 명지대학교 산학협력단 펌프가 필요 없는 비접촉식 전기전도도 측정 기반 미소유체 칩 등전점 전기영동
HUE061595T2 (hu) 2017-12-21 2023-07-28 Ribon Therapeutics Inc Kinazolinonok mint PARP14 inhibitorok

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9509410D0 (en) 1995-05-10 1995-07-05 Imperial College Molecular imaging
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6764817B1 (en) * 1999-04-20 2004-07-20 Target Discovery, Inc. Methods for conducting metabolic analyses
US6676819B1 (en) * 1999-09-14 2004-01-13 Yaoqing Diana Liu Methods and apparatus for automatic on-line multi-dimensional electrophoresis
GB0019496D0 (en) 2000-08-08 2000-09-27 Diamond Optical Tech Ltd System and method
GB0019499D0 (en) 2000-08-08 2000-09-27 Diamond Optical Tech Ltd system and method
GB0019500D0 (en) 2000-08-08 2000-09-27 Diamond Optical Tech Ltd System and method

Also Published As

Publication number Publication date
GB0030708D0 (en) 2001-01-31
ES2339744T3 (es) 2010-05-25
CA2431357A1 (en) 2002-06-20
US6979390B2 (en) 2005-12-27
PT1341806E (pt) 2010-04-16
EP1341806A1 (de) 2003-09-10
EP1341806B1 (de) 2010-01-20
US20040100268A1 (en) 2004-05-27
AU2001295776A1 (en) 2002-06-24
DE60141159D1 (de) 2010-03-11
JP2004525350A (ja) 2004-08-19
WO2002048177A1 (en) 2002-06-20
DK1341806T3 (da) 2010-02-22
JP4054678B2 (ja) 2008-02-27

Similar Documents

Publication Publication Date Title
JP4754759B2 (ja) 化合物の電気泳動分離
ATE295535T1 (de) Verfahren und vorrichtung zur analyse von geladenen molekülen durch isoelektrische fokussierung einer lösung
Ros et al. Protein purification by Off‐Gel electrophoresis
ATE335845T1 (de) Elektrophoretische analyse von molekülen mit immobilisierten sonden
AU2001267455A1 (en) Electrophoretic separation of compounds
DE69800630D1 (de) Chip zur elektroforetischen Trennung von Molekülen und Verfahren zur Verwendung desselben
DE69721585D1 (de) Verfahren zur aufnahme von flüssigen proben für analytische tests
ATE227023T1 (de) Verfahren und vorrichtung zum ausführen von mikrofluidmanipulationen für chemische analyse und synthese
ATE455787T1 (de) Verfahren und vorrichtung zur abtrennung von biomolekülen
Jenkins et al. Identification and quantitation of human urine proteins by capillary electrophoresis
ATE370158T1 (de) Verfahren zur elektrophoretischen auftrennung von membranproteinen
SE9803224D0 (sv) Method for separation of macromolecules
DE69420750D1 (de) Verfahren zur Probenanalyse mittels Kapillarelektroforese
JPH02107960A (ja) 荷電または分極性巨大分子を電気泳動により分離、精製および濃縮するための方法および装置
Sun et al. Enhanced albumin protein separations and protein—drug binding constant measurements using anti-inflammatory drugs as run buffer additives in affinity capillary electrophoresis
DE69941655D1 (de) Elektrophoretischer Trennung von Nukleinsäuren aus biologischen Proben bei niedrigem pH-Wert
ATE197507T1 (de) Verfahren und verbindungen zur detektion von analyten mittels remanenzmessung und deren verwendung
ATE240519T1 (de) Vorrichtung und verfahren zur miniaturisierten, hochparallelen elektrophoretischen trennung
BRPI0406265A (pt) Método para análise eletroforética capilar, métodos para pesquisa e tipagem de proteìnas monoclonais, processo para preparação de anticorpos modificados, anticorpos modificados, kit para detecção e tipagem de proteìnas monoclonais e uso de anticorpos
ATE213227T1 (de) Verfahren und vorrichtung zur aufreinigung und anreicherung von molekülen
ATE274185T1 (de) Verfahren und zusammensetzungen zur trennung von biologischen makromolekülen
WO2002029396A3 (de) Verfahren und vorrichtung zur 2d-elektrophorese in grossen gelen
ATE397658T1 (de) Verfahren zur zweidimensionalen konformationsabhängigen trennung nichtzirkulärer nukleinsäuren
Kaneta et al. On‐column capture of a specific protein separated by SDS‐CGE using an immunological reaction on magnetic beads
Delmotte Separation of proteins and other compounds by capillary isotachophoresis

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1341806

Country of ref document: EP

REN Ceased due to non-payment of the annual fee