ATE424449T1 - Trennung von spermazellen durch elektrophorese - Google Patents

Trennung von spermazellen durch elektrophorese

Info

Publication number
ATE424449T1
ATE424449T1 AT04761402T AT04761402T ATE424449T1 AT E424449 T1 ATE424449 T1 AT E424449T1 AT 04761402 T AT04761402 T AT 04761402T AT 04761402 T AT04761402 T AT 04761402T AT E424449 T1 ATE424449 T1 AT E424449T1
Authority
AT
Austria
Prior art keywords
electrophoresis
separation
sperm
sperm cells
population
Prior art date
Application number
AT04761402T
Other languages
English (en)
Inventor
Robert Aitken
Christopher James Ainsworth
Original Assignee
Univ Newcastle Res Ass
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2003905474A external-priority patent/AU2003905474A0/en
Application filed by Univ Newcastle Res Ass filed Critical Univ Newcastle Res Ass
Application granted granted Critical
Publication of ATE424449T1 publication Critical patent/ATE424449T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/50—Polycarbonates

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT04761402T 2003-10-07 2004-10-07 Trennung von spermazellen durch elektrophorese ATE424449T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU2003905474A AU2003905474A0 (en) 2003-10-07 Gamete Separation

Publications (1)

Publication Number Publication Date
ATE424449T1 true ATE424449T1 (de) 2009-03-15

Family

ID=34397666

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04761402T ATE424449T1 (de) 2003-10-07 2004-10-07 Trennung von spermazellen durch elektrophorese

Country Status (6)

Country Link
US (1) US8123924B2 (de)
EP (1) EP1689853B1 (de)
AT (1) ATE424449T1 (de)
AU (1) AU2004278448B2 (de)
DE (1) DE602004019804D1 (de)
WO (1) WO2005033295A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003902363A0 (en) * 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation
EP2361967A1 (de) * 2010-02-26 2011-08-31 Assistance Publique - Hôpitaux de Paris Verfahren zur Trennung von Gameten, und deren Zusammensetzungen und Verwendungen
CN113969274A (zh) * 2012-03-16 2022-01-25 生育创新有限公司 精细胞的处理
GB201210496D0 (en) * 2012-06-13 2012-07-25 Fertility Innovations Ltd Method and apparatus for sperm enrichment
US9034161B2 (en) 2012-08-23 2015-05-19 Douglas T. Carrell Sperm separation devices and associated methods
ES2757884T3 (es) 2012-08-29 2020-04-30 Inguran Llc Eliminación magnética o identificación de células del esperma dañadas o comprometidas
US10379026B2 (en) 2012-08-29 2019-08-13 Inguran, Llc Cell processing using magnetic particles
EP2717034A1 (de) * 2012-10-08 2014-04-09 Roumen Parapanov Verfahren und Kits zur Qualitätsbeurteilung von Spermien und Verwendungen davon
EP3528935A4 (de) * 2016-10-20 2020-05-20 Memphasys Limited Spermientrennung durch elektrophorese
JP7097900B2 (ja) * 2016-10-20 2022-07-08 メンファシス リミテッド 電気泳動デバイス
CA3089148A1 (en) 2017-01-31 2018-08-09 Genea Ip Holdings Pty Limited Method and system for processing a biological sample
CN110967230B (zh) * 2019-11-22 2022-05-31 珠海高瑞特医疗科技有限公司 一种精子活性氧含量的测定方法及试剂盒
EP3851853A1 (de) * 2020-01-17 2021-07-21 Biotechnologicky Ustav AV CR, v.v.i. Verfahren zur trennung von spermien mit unbeschädigtem intaktem kopf von spermien mit beschädigtem kopf und somatischen zellen
CN111323361B (zh) * 2020-03-17 2022-05-10 南通大学 一种快速分离精子头、精子尾和正常有活力精子的方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4323439A (en) 1979-12-31 1982-04-06 The Regents Of The University Of California Method and apparatus for dynamic equilibrium electrophoresis
US5071536A (en) 1985-11-25 1991-12-10 Ivory Cornelius F High resolution continuous flow electrophoresis
US6001617A (en) * 1989-06-07 1999-12-14 Queen's University At Kingston Electroporation device and method of use
US5336387A (en) 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US5296375A (en) * 1992-05-01 1994-03-22 Trustees Of The University Of Pennsylvania Mesoscale sperm handling devices
US5662813A (en) 1994-10-21 1997-09-02 Bioseparations, Inc. Method for separation of nucleated fetal erythrocytes from maternal blood samples
TW391881B (en) 1996-09-25 2000-06-01 Baxter Int Method and apparatus for filtering suspensions of medical and biological fluids or the like
US6824995B1 (en) 1998-03-03 2004-11-30 Emory University Diagnostic for metastatic prostate cancer
AU3146800A (en) * 1999-03-05 2000-09-28 De Danske Kvaegavlsforeninger Determination of sperm concentration and viability for artificial insemination
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
WO2002000100A2 (en) 2000-06-23 2002-01-03 Daniel Armstrong Method for separation, identification and evaluation of microbes and cells
US6923896B2 (en) 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
SE521371C2 (sv) 2000-09-25 2003-10-28 Sca Hygiene Prod Ab Absorberande alster med midjebälte
US20020119218A1 (en) * 2000-12-21 2002-08-29 Burke Earl P. Method & apparatus of electric cleansing of food products
US6991715B2 (en) * 2001-05-15 2006-01-31 Gradipore Limited Prion diagnostic test
DE10149875A1 (de) * 2001-10-10 2003-07-03 Alpha Technology Ges Fuer Ange Vorrichtung zur Extraktion elektrisch geladener Moleküle
AU2003902363A0 (en) 2003-05-15 2003-05-29 Life Therapeutics Limited Cell separation

Also Published As

Publication number Publication date
US8123924B2 (en) 2012-02-28
EP1689853B1 (de) 2009-03-04
AU2004278448A1 (en) 2005-04-14
AU2004278448B2 (en) 2007-06-07
DE602004019804D1 (de) 2009-04-16
EP1689853A1 (de) 2006-08-16
WO2005033295A1 (en) 2005-04-14
US20090101507A1 (en) 2009-04-23
EP1689853A4 (de) 2007-07-11

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Legal Events

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RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties