AR010780A1 - Un metodo para la separacion de una celula objetivo de una muestra y la composicion obtenida - Google Patents

Un metodo para la separacion de una celula objetivo de una muestra y la composicion obtenida

Info

Publication number
AR010780A1
AR010780A1 ARP970105903A ARP970105903A AR010780A1 AR 010780 A1 AR010780 A1 AR 010780A1 AR P970105903 A ARP970105903 A AR P970105903A AR P970105903 A ARP970105903 A AR P970105903A AR 010780 A1 AR010780 A1 AR 010780A1
Authority
AR
Argentina
Prior art keywords
fatty
separation
desired target
proteins
sample
Prior art date
Application number
ARP970105903A
Other languages
English (en)
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Publication of AR010780A1 publication Critical patent/AR010780A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G11/00—Compounds of cadmium
    • C01G11/003—Preparation involving a liquid-liquid extraction, an adsorption or an ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B1/00—Preparatory treatment of cellulose for making derivatives thereof, e.g. pre-treatment, pre-soaking, activation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se describe un método para la separacion de una molécula objetivo de una mezcla. El método emplea cuerpos grasos y sus proteínas asociadas como matricesde afinidad para el enlace no covalente selectivo de las moléculas objetivo deseadas. Lasp roteínas de cuerpo graso pueden estar fusionadas genéticamentea un ligando con especificidad para la molécula objetivo deseada. Las proteínas de cuerpo graso nativas se pueden utilizar también enconjunto conun ligando específico deproteína de cuerpo graso o una proteína de enlace de cuerpo graso. El método permite la separacion y recuperacion de las moléculasobjetivo deseadas debido a diferencias en densidades entre los cuerpos grasos y las soluciones acuosas. Tambiéncontempla un a composicion que comprendecuerpos grasos asociados con una molécula.
ARP970105903A 1996-12-16 1997-12-16 Un metodo para la separacion de una celula objetivo de una muestra y la composicion obtenida AR010780A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/767,026 US5856452A (en) 1996-12-16 1996-12-16 Oil bodies and associated proteins as affinity matrices

Publications (1)

Publication Number Publication Date
AR010780A1 true AR010780A1 (es) 2000-07-12

Family

ID=25078275

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP970105903A AR010780A1 (es) 1996-12-16 1997-12-16 Un metodo para la separacion de una celula objetivo de una muestra y la composicion obtenida

Country Status (19)

Country Link
US (5) US5856452A (es)
EP (1) EP1007554B1 (es)
JP (2) JP3833719B2 (es)
KR (1) KR100421935B1 (es)
CN (1) CN1325513C (es)
AR (1) AR010780A1 (es)
AT (1) ATE321777T1 (es)
AU (1) AU739339B2 (es)
BR (1) BR9713727B1 (es)
CA (1) CA2275489C (es)
DE (1) DE69735599T2 (es)
DK (1) DK1007554T3 (es)
ES (1) ES2259193T3 (es)
HK (1) HK1025977A1 (es)
IL (1) IL130403A (es)
NZ (1) NZ336558A (es)
TW (1) TW589322B (es)
WO (1) WO1998027115A1 (es)
ZA (1) ZA9711237B (es)

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Also Published As

Publication number Publication date
KR100421935B1 (ko) 2004-03-10
US20030096320A1 (en) 2003-05-22
US6924363B1 (en) 2005-08-02
JP3833719B2 (ja) 2006-10-18
EP1007554A1 (en) 2000-06-14
DK1007554T3 (da) 2006-05-01
CA2275489A1 (en) 1998-06-25
ATE321777T1 (de) 2006-04-15
AU739339B2 (en) 2001-10-11
JP2001506241A (ja) 2001-05-15
IL130403A (en) 2005-05-17
CN1245503A (zh) 2000-02-23
NZ336558A (en) 2001-08-31
HK1025977A1 (en) 2000-12-01
US20030059910A1 (en) 2003-03-27
US6509453B1 (en) 2003-01-21
KR20000069499A (ko) 2000-11-25
DE69735599T2 (de) 2006-08-31
US5856452A (en) 1999-01-05
US7332587B2 (en) 2008-02-19
IL130403A0 (en) 2000-06-01
BR9713727B1 (pt) 2009-01-13
CA2275489C (en) 2007-03-27
AU5220498A (en) 1998-07-15
EP1007554B1 (en) 2006-03-29
JP2006306881A (ja) 2006-11-09
ZA9711237B (en) 1998-07-06
DE69735599D1 (de) 2006-05-18
BR9713727A (pt) 2000-01-25
CN1325513C (zh) 2007-07-11
ES2259193T3 (es) 2006-09-16
TW589322B (en) 2004-06-01
WO1998027115A1 (en) 1998-06-25

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