IL130403A - Oil bodies and associated proteins as affinity matrices - Google Patents

Oil bodies and associated proteins as affinity matrices

Info

Publication number
IL130403A
IL130403A IL13040397A IL13040397A IL130403A IL 130403 A IL130403 A IL 130403A IL 13040397 A IL13040397 A IL 13040397A IL 13040397 A IL13040397 A IL 13040397A IL 130403 A IL130403 A IL 130403A
Authority
IL
Israel
Prior art keywords
oil body
oil
oil bodies
associated proteins
affinity matrices
Prior art date
Application number
IL13040397A
Other versions
IL130403A0 (en
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Publication of IL130403A0 publication Critical patent/IL130403A0/en
Publication of IL130403A publication Critical patent/IL130403A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G11/00—Compounds of cadmium
    • C01G11/003—Preparation involving a liquid-liquid extraction, an adsorption or an ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B1/00—Preparatory treatment of cellulose for making derivatives thereof, e.g. pre-treatment, pre-soaking, activation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Inorganic Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Materials Engineering (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for the separation of a target molecule from a mixture is described. The method employs oil bodies and their associated proteins as affinity matrices for the selective, non-covalent binding of desired target molecules. The oil body proteins may be genetically fused to a ligand having specificity for the desired target molecule. Native oil body proteins can also be used in conjunction with an oil body protein specific ligand such as an antibody or an oil body binding protein. The method allows the separation and recovery of the desired target molecules due to the difference in densities between oil bodies and aqueous solutions.
IL13040397A 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices IL130403A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/767,026 US5856452A (en) 1996-12-16 1996-12-16 Oil bodies and associated proteins as affinity matrices
PCT/CA1997/000951 WO1998027115A1 (en) 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices

Publications (2)

Publication Number Publication Date
IL130403A0 IL130403A0 (en) 2000-06-01
IL130403A true IL130403A (en) 2005-05-17

Family

ID=25078275

Family Applications (1)

Application Number Title Priority Date Filing Date
IL13040397A IL130403A (en) 1996-12-16 1997-12-05 Oil bodies and associated proteins as affinity matrices

Country Status (19)

Country Link
US (5) US5856452A (en)
EP (1) EP1007554B1 (en)
JP (2) JP3833719B2 (en)
KR (1) KR100421935B1 (en)
CN (1) CN1325513C (en)
AR (1) AR010780A1 (en)
AT (1) ATE321777T1 (en)
AU (1) AU739339B2 (en)
BR (1) BR9713727B1 (en)
CA (1) CA2275489C (en)
DE (1) DE69735599T2 (en)
DK (1) DK1007554T3 (en)
ES (1) ES2259193T3 (en)
HK (1) HK1025977A1 (en)
IL (1) IL130403A (en)
NZ (1) NZ336558A (en)
TW (1) TW589322B (en)
WO (1) WO1998027115A1 (en)
ZA (1) ZA9711237B (en)

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Also Published As

Publication number Publication date
KR100421935B1 (en) 2004-03-10
US20030096320A1 (en) 2003-05-22
US6924363B1 (en) 2005-08-02
JP3833719B2 (en) 2006-10-18
EP1007554A1 (en) 2000-06-14
DK1007554T3 (en) 2006-05-01
CA2275489A1 (en) 1998-06-25
ATE321777T1 (en) 2006-04-15
AU739339B2 (en) 2001-10-11
JP2001506241A (en) 2001-05-15
CN1245503A (en) 2000-02-23
NZ336558A (en) 2001-08-31
HK1025977A1 (en) 2000-12-01
US20030059910A1 (en) 2003-03-27
US6509453B1 (en) 2003-01-21
AR010780A1 (en) 2000-07-12
KR20000069499A (en) 2000-11-25
DE69735599T2 (en) 2006-08-31
US5856452A (en) 1999-01-05
US7332587B2 (en) 2008-02-19
IL130403A0 (en) 2000-06-01
BR9713727B1 (en) 2009-01-13
CA2275489C (en) 2007-03-27
AU5220498A (en) 1998-07-15
EP1007554B1 (en) 2006-03-29
JP2006306881A (en) 2006-11-09
ZA9711237B (en) 1998-07-06
DE69735599D1 (en) 2006-05-18
BR9713727A (en) 2000-01-25
CN1325513C (en) 2007-07-11
ES2259193T3 (en) 2006-09-16
TW589322B (en) 2004-06-01
WO1998027115A1 (en) 1998-06-25

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