AR006937A1 - Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y - Google Patents

Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y

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Publication number
AR006937A1
AR006937A1 ARP970101812A ARP970101812A AR006937A1 AR 006937 A1 AR006937 A1 AR 006937A1 AR P970101812 A ARP970101812 A AR P970101812A AR P970101812 A ARP970101812 A AR P970101812A AR 006937 A1 AR006937 A1 AR 006937A1
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AR
Argentina
Prior art keywords
vector
trehalose
cells
organs
tissues
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ARP970101812A
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English (en)
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Syngenta Mogen Bv
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Publication date
Application filed by Syngenta Mogen Bv filed Critical Syngenta Mogen Bv
Publication of AR006937A1 publication Critical patent/AR006937A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
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    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01028—Alpha,alpha-trehalase (3.2.1.28)

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  • Health & Medical Sciences (AREA)
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  • Plant Pathology (AREA)
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  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Cereal-Derived Products (AREA)
  • Saccharide Compounds (AREA)

Abstract

Regulación de flujo de carbono en el metabolismo de las células. Se ha hallado que la inducción de un cambio en la disponibilidad intracelular delsacárico trehalosa-6-fosfato (T-6-P) induce modificaciones en el desarrollo y/o composición delascélulas, tejidos y organos in vivo. Estos cambios puedenser inducidos por la inducción o inhibición de la enzima trehalosa fosfato sintasa (TPS) que es capaz de formar T-6-P y trehalosa fosfato fosfatasa (TPP)que degrada T-6-P a trehalosa.El flujo de carbono a través de la glucólisis sera estimulado por una disminución del nivel intracelular de T-6-P. Asíse refiere a un método de modificación del desarrollo y/o composición de células, tejidos u órganos in vivo por medio dela inducción de unamodificación en la disponibilidad metabólica de trehalosa-6-fosfato. También se refiere a un vector de clonación utilizado en dicho método, a una plantao parte de planta transformada con dicho vector, a un organismo huésped quecomprende a dicho vector, a una célula transformada con dicho organismo, ya polinucleótidos que codifican trehalosa-6-fosfato sintasa y trehalosa-6- fosfato fosfatasa.
ARP970101812A 1996-05-03 1997-05-02 Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y AR006937A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96201225 1996-05-03
EP96202128 1996-07-26
EP96202395 1996-08-29

Publications (1)

Publication Number Publication Date
AR006937A1 true AR006937A1 (es) 1999-09-29

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ARP970101812A AR006937A1 (es) 1996-05-03 1997-05-02 Metodo de modificacion del desarrollo y/o composicion de celulas, tejidos u organos in vivo, un vector de clonacion utilizado en dicho metodo, plantao parte de planta transformada con dicho vector, organismo huesped que comprende a dicho vector, celula transformada con dicho organismo, y

Country Status (24)

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US (4) US6833490B1 (es)
EP (1) EP0901527B1 (es)
JP (1) JP2000510691A (es)
KR (1) KR20000010758A (es)
CN (1) CN1267558C (es)
AR (1) AR006937A1 (es)
AT (1) ATE301718T1 (es)
AU (2) AU718379B2 (es)
BR (1) BR9710959A (es)
CA (1) CA2253348C (es)
CZ (1) CZ345098A3 (es)
DE (1) DE69733945T2 (es)
DK (1) DK0901527T3 (es)
ES (1) ES2243997T3 (es)
IL (1) IL126781A0 (es)
IN (1) IN1997CH00924A (es)
MA (1) MA24248A1 (es)
MX (1) MX9809128A (es)
NO (1) NO985097L (es)
NZ (1) NZ332677A (es)
PL (1) PL329913A1 (es)
SK (1) SK148498A3 (es)
TR (4) TR199902037T2 (es)
WO (1) WO1997042326A2 (es)

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US6833490B1 (en) 2004-12-21
US20040093641A1 (en) 2004-05-13
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