ZA881534B - Novel fusion proteins and their purification - Google Patents

Novel fusion proteins and their purification

Info

Publication number
ZA881534B
ZA881534B ZA881534A ZA881534A ZA881534B ZA 881534 B ZA881534 B ZA 881534B ZA 881534 A ZA881534 A ZA 881534A ZA 881534 A ZA881534 A ZA 881534A ZA 881534 B ZA881534 B ZA 881534B
Authority
ZA
South Africa
Prior art keywords
purification
fusion proteins
novel fusion
novel
affinity chromatography
Prior art date
Application number
ZA881534A
Other languages
English (en)
Inventor
Doebeli Heinz
Eggimann Bernhard
Gentz Reiner
Hochuli Erich
Stueber Dietrich
Original Assignee
F. Hoffmann-La Roche & Co. Aktiengesellschaft
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F. Hoffmann-La Roche & Co. Aktiengesellschaft filed Critical F. Hoffmann-La Roche & Co. Aktiengesellschaft
Publication of ZA881534B publication Critical patent/ZA881534B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
ZA881534A 1987-03-10 1988-03-03 Novel fusion proteins and their purification ZA881534B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH89587 1987-03-10

Publications (1)

Publication Number Publication Date
ZA881534B true ZA881534B (en) 1988-09-12

Family

ID=4197678

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA881534A ZA881534B (en) 1987-03-10 1988-03-03 Novel fusion proteins and their purification

Country Status (11)

Country Link
US (2) US5284933A (xx)
EP (1) EP0282042B1 (xx)
JP (1) JP2686090B2 (xx)
AT (1) ATE106897T1 (xx)
AU (1) AU609783B2 (xx)
CA (1) CA1340522C (xx)
DE (1) DE3889949D1 (xx)
DK (1) DK173951B1 (xx)
IE (1) IE63991B1 (xx)
NZ (1) NZ223735A (xx)
ZA (1) ZA881534B (xx)

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US5047333A (en) * 1987-12-22 1991-09-10 Eniricerche S.P.A. Method for the preparation of natural human growth hormone in pure form

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5047333A (en) * 1987-12-22 1991-09-10 Eniricerche S.P.A. Method for the preparation of natural human growth hormone in pure form

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DE3889949D1 (de) 1994-07-14
IE880685L (en) 1988-09-10
JP2686090B2 (ja) 1997-12-08
EP0282042A3 (de) 1991-09-11
US5284933A (en) 1994-02-08
US5310663A (en) 1994-05-10
NZ223735A (en) 1990-10-26
CA1340522C (en) 1999-05-04
JPS63251095A (ja) 1988-10-18
EP0282042B1 (de) 1994-06-08
AU1270988A (en) 1988-09-15
AU609783B2 (en) 1991-05-09
EP0282042A2 (de) 1988-09-14
DK84288D0 (da) 1988-02-18
DK173951B1 (da) 2002-03-04
ATE106897T1 (de) 1994-06-15
IE63991B1 (en) 1995-06-28
DK84288A (da) 1988-09-11

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