DK84288D0 - Fusionsproteiner og oprensning deraf - Google Patents

Fusionsproteiner og oprensning deraf

Info

Publication number
DK84288D0
DK84288D0 DK084288A DK84288A DK84288D0 DK 84288 D0 DK84288 D0 DK 84288D0 DK 084288 A DK084288 A DK 084288A DK 84288 A DK84288 A DK 84288A DK 84288 D0 DK84288 D0 DK 84288D0
Authority
DK
Denmark
Prior art keywords
purification
fusion proteins
affinity chromatography
metal chelate
chelate affinity
Prior art date
Application number
DK084288A
Other languages
English (en)
Other versions
DK84288A (da
DK173951B1 (da
Inventor
Heinz Doebeli
Bernhard Eggimann
Erich Hochuli
Dietrich Stueber
Reiner Gentz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of DK84288D0 publication Critical patent/DK84288D0/da
Publication of DK84288A publication Critical patent/DK84288A/da
Application granted granted Critical
Publication of DK173951B1 publication Critical patent/DK173951B1/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
DK198800842A 1987-03-10 1988-02-18 Fusionsproteiner, gener der koder herfor, ekspressionsvektorer, transformeret bakterie, fremgangsmåde til oprensning af fusionsproteiner, fremgangsmåde til oprensning af et biologisk aktivt polypeptid eller protein, vacciner indeholdende fusionsprotei... DK173951B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CH89587 1987-03-10
CH89587 1987-03-10

Publications (3)

Publication Number Publication Date
DK84288D0 true DK84288D0 (da) 1988-02-18
DK84288A DK84288A (da) 1988-09-11
DK173951B1 DK173951B1 (da) 2002-03-04

Family

ID=4197678

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198800842A DK173951B1 (da) 1987-03-10 1988-02-18 Fusionsproteiner, gener der koder herfor, ekspressionsvektorer, transformeret bakterie, fremgangsmåde til oprensning af fusionsproteiner, fremgangsmåde til oprensning af et biologisk aktivt polypeptid eller protein, vacciner indeholdende fusionsprotei...

Country Status (11)

Country Link
US (2) US5284933A (da)
EP (1) EP0282042B1 (da)
JP (1) JP2686090B2 (da)
AT (1) ATE106897T1 (da)
AU (1) AU609783B2 (da)
CA (1) CA1340522C (da)
DE (1) DE3889949D1 (da)
DK (1) DK173951B1 (da)
IE (1) IE63991B1 (da)
NZ (1) NZ223735A (da)
ZA (1) ZA881534B (da)

Families Citing this family (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8706599D0 (en) * 1987-03-19 1987-04-23 Hoffmann La Roche Plasmodium falciparum merozoite antigen peptides
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
DE3804148A1 (de) * 1988-02-11 1989-08-24 Andreas Dr Plueckthun Verfahren zur spaltung von fusionsproteinen
US5919665A (en) 1989-10-31 1999-07-06 Ophidian Pharmaceuticals, Inc. Vaccine for clostridium botulinum neurotoxin
WO1991010910A2 (de) * 1990-01-19 1991-07-25 Boehringer Mannheim Gmbh Verfahren zur enzymatischen behandlung von substraten
US5594115A (en) * 1990-04-09 1997-01-14 Pharmacia & Upjohn Company Process of purifying recombinant proteins and compounds useful in such process
ATE139572T1 (de) * 1990-04-09 1996-07-15 Upjohn Co Verfahren zur reinigung von rekombinanten proteinen und dafür nützliche produkte
CA2094512A1 (en) * 1990-12-13 1992-06-14 Teresa M. Kubiak Fusion polypeptides
US5439829A (en) * 1991-01-30 1995-08-08 Eli Lilly And Company Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion
US5646016A (en) * 1991-02-06 1997-07-08 Genetics Institute, Inc. Peptide and protein fusions to thioredoxin, thioredoxin-like molecules, and modified thioredoxin-like molecules
FR2685347A1 (fr) * 1991-12-23 1993-06-25 Univ Pasteur Procede biotechnologique d'obtention et de production microbienne d'oligomeres peptidiques comme substituts de la gelatine.
DE4237244A1 (de) * 1992-11-04 1994-05-05 Elias Entwicklungslabor Fuer I Verfahren zur Herstellung hochreiner humaner GAD-1- und GAD-2- Proteine
KR100310739B1 (ko) * 1993-02-04 2002-05-30 알란 스코브 단백질재생을위한개선된방법
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
CA2125467C (en) * 1993-07-06 2001-02-06 Heinz Dobeli Process for producing hydrophobic polypeptides, proteins or peptides
GB2284208A (en) * 1993-11-25 1995-05-31 Pna Diagnostics As Nucleic acid analogues with a chelating functionality for metal ions
US5643757A (en) * 1994-03-21 1997-07-01 American Cyanamid Company High yield production of human apolipoprotein A1 in E. coli.
DE4433980C2 (de) * 1994-09-23 1996-08-22 Boehringer Ingelheim Int Verfahren und Biosensorhit zur Untersuchung der Wechselwirkung von Biomolekülen mittels Oberflächen-Plasma-Resonanz
US6472148B1 (en) 1994-09-26 2002-10-29 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
US5620850A (en) * 1994-09-26 1997-04-15 President And Fellows Of Harvard College Molecular recognition at surfaces derivatized with self-assembled monolayers
CA2203504A1 (en) * 1994-10-24 1996-05-02 James A. Williams Vaccine and antitoxin for treatment and prevention of c. difficile disease
US6967088B1 (en) * 1995-03-16 2005-11-22 Allergan, Inc. Soluble recombinant botulinum toxin proteins
ES2096529B1 (es) * 1995-04-26 1997-11-16 Univ Madrid Complutense Procedimiento para la preparacion y purificacion por cromatografia de afinidad, de la proteina codificada por la orf-5 del virus del sindrome reproductivo y respiratorio porcino.
DE19536166C1 (de) 1995-09-29 1997-03-06 Siegfried Dr Krell Verfahren zur Bestimmung von Antikörpern gegen Treponema pallidum (Syphilis)
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
DE69730551T2 (de) 1996-04-15 2005-09-01 Givaudan S.A. Hydroxyperoxide Lyasen
ES2108652B1 (es) * 1996-04-19 1998-07-16 Antibioticos Sa Un procedimiento para modificar la enzima 7/3-(4-carboxibutanamido) cefalosporinacilasa y purificarla en un solo paso cromatografico.
EP0937148A1 (en) * 1996-04-19 1999-08-25 Henry M. Jackson Foundation For The Advancement Of Military Medicine Histidine-tagged intimin and methods of using intimin to stimulate an immune response and as an antigen carrier with targeting capability
WO1997040177A1 (en) 1996-04-19 1997-10-30 Henry M. Jackson Foundation For The Advancement Of Military Medicine Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens
US5948889A (en) * 1996-05-21 1999-09-07 Case Western Reserve University Compositions and methods for screening antimicrobials
EP0821006B1 (de) 1996-07-26 2004-04-21 Aventis Pharma Deutschland GmbH Insulinderivate mit erhöhter Zinkbindung
US7014992B1 (en) 1996-11-05 2006-03-21 Clinical Micro Sensors, Inc. Conductive oligomers attached to electrodes and nucleoside analogs
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
US6455323B1 (en) * 1997-07-03 2002-09-24 Pharmacia & Upjohn Company Anti-bacterial methods and materials
US6183740B1 (en) 1997-08-13 2001-02-06 Diversa Corporation Recombinant bacterial phytases and uses thereof
US6855365B2 (en) 1997-08-13 2005-02-15 Diversa Corporation Recombinant bacterial phytases and uses thereof
US7078035B2 (en) * 1997-08-13 2006-07-18 Diversa Corporation Phytases, nucleic acids encoding them and methods for making and using them
US20040091968A1 (en) * 1997-08-13 2004-05-13 Short Jay M. Recombinant phytases and methods of making and using them
US7432097B2 (en) * 1997-08-13 2008-10-07 Verenium Corporation Phytases, nucleic acids encoding them and methods of making and using them
US6720014B1 (en) * 1997-08-13 2004-04-13 Diversa Corporation Phytase-containing foodstuffs and methods of making and using them
ES2131018B1 (es) 1997-09-25 2000-04-01 Antibioticos Sau Un procedimiento enzimatico para preparar acido 7beta-(4-carboxibutanamido)cefalosporanico utilizando la enzima d-aminoacido oxidasa de trigonopsis variabilis modificada producida en escherichia coli.
US6444442B1 (en) * 1997-12-30 2002-09-03 Universidade Federal De Minas Gerais-Ufmg Method of producing a recombinant non-glycosylated gp90 of equine infectious anemia virus (EIAV), and product thereof
CA2222993A1 (en) 1998-02-04 1999-08-04 The Ontario Cancer Institute A method for using a ribosome-inactivating protein complex as a structural template and a molecular search engine in the design, construction and screening of combinatorial protein libraries
DE19819843A1 (de) * 1998-05-05 1999-11-11 Biotechnolog Forschung Gmbh Metallchelat bindende Peptide
DE19822823A1 (de) * 1998-05-20 2000-02-10 Basf Ag Neue Peptidfragmente zur Reinigung von Proteinen
DE19825447A1 (de) 1998-06-06 1999-12-09 Hoechst Marion Roussel De Gmbh Neue Insulinanaloga mit erhöhter Zinkbildung
US20070161010A1 (en) * 1998-08-04 2007-07-12 Gjerde Douglas T Methods and compositions for mutation analysis
ATE253075T1 (de) * 1998-08-27 2003-11-15 Bayer Ag Oligomerreinigung durch selektion mittels zweier enden
US7176298B2 (en) 1998-09-25 2007-02-13 Clontech Laboratories, Inc. Polynucleotides encoding metal ion affinity peptides and related products
US6479300B1 (en) 1999-03-15 2002-11-12 Millipore Corporation Metal loaded ligand bound membranes for metal ion affinity chromatography
US6790950B2 (en) 1999-04-09 2004-09-14 Pharmacia & Upjohn Company Anti-bacterial vaccine compositions
ES2535961T3 (es) * 1999-04-09 2015-05-19 Zoetis P&U Llc Bacteria pasteurelácea atenuada que tiene una mutación en un gen de virulencia
ATE320443T1 (de) * 1999-07-15 2006-04-15 Qiagen Gmbh Verfahren zur trennung von teilchenförmigen substraten aus einer lösung indem man den teilchenverlust minimiert
US6379903B1 (en) 1999-10-08 2002-04-30 Sigma-Aldrich Co. Purification of recombinant proteins fused to multiple epitopes
US20020100042A1 (en) * 2000-01-19 2002-07-25 Denis Khoo Method and system for providing intelligent advertisement placement in a motion picture
US6764823B2 (en) 2000-04-06 2004-07-20 Pharmacia & Upjohn Company Antimicrobial methods and materials
US6623655B1 (en) * 2000-04-24 2003-09-23 Sigma-Aldrich Co. Metal chelating compositions
EP1328658A2 (en) * 2000-06-22 2003-07-23 San Diego State University Foundation Recombination modulators and methods for their production and use
US7592008B2 (en) 2000-11-20 2009-09-22 The Board Of Trustees Of The University Of Illinois, A Body Corporate And Politic Of The State Of Illinois Membrane scaffold proteins
JP4061043B2 (ja) * 2000-12-28 2008-03-12 株式会社ポストゲノム研究所 ｉｎｖｉｔｒｏ転写／翻訳系によるペプチド等の製造方法
DE10113776B4 (de) * 2001-03-21 2012-08-09 "Iba Gmbh" Isoliertes streptavidinbindendes, kompetitiv eluierbares Peptid, dieses umfassendes Fusionspeptid, dafür codierende Nukleinsäure, Expressionsvektor, Verfahren zur Herstellung eines rekombinanten Fusionsproteins und Verfahren zum Nachweis und/oder zur Gewinnung des Fusionsproteins
WO2002079493A2 (en) * 2001-03-29 2002-10-10 Hybrigen, Inc. Improved hybrid gene libraries and uses thereof
US20050176070A1 (en) * 2001-04-05 2005-08-11 Auton Kevin A. Protein analysis
US20040180415A1 (en) * 2001-05-15 2004-09-16 Tchaga Grigoriy S. Methods and compositions for protein purification
US20040002058A1 (en) * 2001-06-21 2004-01-01 Uab Research Foundation Chimeric capsid proteins and uses thereof
WO2003000855A2 (en) * 2001-06-21 2003-01-03 Uab Research Foundation Chimeric capsid proteins and uses thereof
CA2447594A1 (en) 2001-06-21 2003-01-03 Clontech Laboratories, Inc. Water-soluble polymeric metal ion affinity compositions and methods for using the same
US20030087321A1 (en) * 2001-09-28 2003-05-08 Tomich Che-Shen C. Antimicrobial methods and materials
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
US20050272089A1 (en) * 2001-10-19 2005-12-08 Mott John E Critical genes and polypeptides of haemophilus influenzae and methods of use
CA2465912A1 (en) * 2001-11-09 2003-05-22 Dana-Farber Cancer Institute, Inc. Eit-6, a polypeptide encoded by an estrogen regulated gene
JP2005511755A (ja) * 2001-12-05 2005-04-28 ラージ スケール バイオロジー コーポレーション 複数種類のタンパク質を高スループットで産生および精製するための、融通性の高い方法および装置
US6844426B2 (en) * 2002-02-06 2005-01-18 Toyo Boseki Kabushiki Kaisha Magnetic carrier capable of binding with protein and purification method of protein utilizing the magnetic carrier
US7799561B2 (en) * 2002-06-12 2010-09-21 Sigma-Aldrich, Co. Affinity peptides and method for purification of recombinant proteins
US8029803B2 (en) 2002-06-20 2011-10-04 Paladin Labs, Inc. Chimeric antigens for eliciting an immune response
US8025873B2 (en) 2002-06-20 2011-09-27 Paladin Labs, Inc. Chimeric antigens for eliciting an immune response
EP1546359A4 (en) 2002-09-12 2007-05-09 Molecular Probes Inc JOB-SPECIFIC MARKING OF AFFINITY DAYS IN FUSION PROTEINS
JP4078247B2 (ja) * 2003-05-02 2008-04-23 キヤノン株式会社 磁性体−生体物質複合体型構造体、磁性体に対して結合能を有するアミノ酸配列を有するペプチド断片及びその遺伝子、ならびに磁性体−生体物質複合体型構造体の製造方法
CN1813059A (zh) * 2003-05-02 2006-08-02 西格玛-奥尔德利希公司 固相细胞裂解和捕获平台
US20060030007A1 (en) * 2003-05-30 2006-02-09 Byrd Devon R Peptides for metal ion affinity chromatography
US7086750B2 (en) * 2003-06-23 2006-08-08 Se-Kure Controls, Inc. System for generating a message
US8007805B2 (en) * 2003-08-08 2011-08-30 Paladin Labs, Inc. Chimeric antigens for breaking host tolerance to foreign antigens
US20050064547A1 (en) * 2003-09-24 2005-03-24 Brown Arthur M. Vectors and transfected cells
DE602004025192D1 (de) * 2003-12-05 2010-03-11 Roche Diagnostics Gmbh Rekombinante Carboxypeptidase B und ihre Aufreinigung
US20050123932A1 (en) * 2003-12-09 2005-06-09 Mekbib Astatke Nucleic acid-chelating agent conjugates
US7276362B2 (en) * 2004-01-30 2007-10-02 Roche Diagnostics Operations, Inc. Recombinant histidine-tagged inosine monophosphate dehydrogenase polypeptides
ES2245867B1 (es) * 2004-02-05 2007-04-01 Universidad Publica De Navarra Procedimiento produccion sacarosa sintasa recombinante a partir de escherichia coli y uso en fabricacion de kits de determinacion de sacarosa, produccion de azucares-nucleotidos y obtencion de plantas transgenicas con alto contenido de almidon y alto balance amilosa/amilopectina.
WO2005116063A1 (en) 2004-05-24 2005-12-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Live, oral vaccine for protection against shigella dysenteriae serotype 1
DE102004038134B4 (de) 2004-08-05 2013-07-25 Johann Wolfgang Goethe-Universität Frankfurt am Main Multivalente Chelatoren zum Modifizieren und Organisieren von Zielmolekülen, Verfahren zu ihrer Herstellung sowie ihre Verwendung
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
US7294614B2 (en) * 2004-10-12 2007-11-13 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
US7582475B2 (en) 2005-01-07 2009-09-01 University Of Georgia Research Foundation, Inc. Vectors and methods for high throughput co-expression
US7229769B2 (en) * 2005-03-25 2007-06-12 Illumina, Inc. Compositions and methods for detecting protease activity
KR20120089475A (ko) 2005-04-29 2012-08-10 인펙셔스 디지즈 리서치 인스티튜트 (아이디알아이) 결핵균 감염을 예방 또는 치료하기 위한 신규한 방법
US20060251619A1 (en) * 2005-05-04 2006-11-09 Gilles Borrelly Modified interferon-gamma polypeptides and methods for using modified interferon-gamma polypeptides
US7741053B2 (en) * 2005-05-13 2010-06-22 Sigma-Aldrich Co. Processes for purification of recombinant proteins
US7265213B2 (en) * 2005-07-28 2007-09-04 Kpl, Inc. Methodology of conjugating chelators to biomolecules
EP1924601B1 (en) * 2005-08-30 2011-10-19 Novo Nordisk A/S Expression of proteins in e.coli
ZA200804078B (en) * 2005-10-13 2009-09-30 Virexx Medical Corp Chimeric hepatitis C virus antigens for eliciting an immune response
US7462750B2 (en) 2005-10-31 2008-12-09 Specialty Coating Systems, Inc. Parylene variants and methods of synthesis and use
WO2007084388A2 (en) * 2006-01-12 2007-07-26 Sessa William C Nogo-b receptor
EP1981978B1 (en) * 2006-02-08 2011-01-19 USV Limited Affinity polypeptide for purification of recombinant proteins
WO2007120999A2 (en) * 2006-03-06 2007-10-25 Bio-Rad Laboratories, Inc. Chelating monomers and polymers
GB0610479D0 (en) * 2006-05-26 2006-07-05 Ge Healthcare Bio Sciences Ab A method for generating metal chelating affinity ligands
WO2008060776A2 (en) * 2006-10-03 2008-05-22 University Of Medicine And Dentistry Of New Jersey Atap peptides, nucleic acids encoding the same and associated methods of use
JP2010519894A (ja) * 2007-03-01 2010-06-10 ノボ・ノルデイスク・エー／エス 大腸菌におけるタンパク質の発現
US8536302B2 (en) 2007-08-27 2013-09-17 Sysmex Corporation Dockerin polypeptide and method of purifying recombinant fused protein using the same
EP2209494B1 (en) * 2007-10-09 2016-07-20 Polytherics Limited Novel conjugated proteins and peptides
US20090162845A1 (en) 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
JP5260976B2 (ja) * 2008-02-08 2013-08-14 東ソー株式会社 血液凝固第ｖｉｉｉ因子ｃ２ドメインタンパク質の製造方法
AU2009242546B2 (en) 2008-04-30 2015-01-22 Integrated Dna Technologies, Inc. RNase-H-based assays utilizing modified RNA monomers
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
GB0823550D0 (en) 2008-12-24 2009-01-28 Kinasource Ltd Peptide tag and uses thereof
NZ596459A (en) 2009-05-21 2013-11-29 Verenium Corp Phytases, nucleic acids encoding them and methods for making and using them
US8304248B2 (en) 2009-11-16 2012-11-06 Torres Anthony R Protein separation via ion-exchange chromatography and associated methods, systems, and devices
US8945307B2 (en) 2009-12-23 2015-02-03 Aeroquest Research Group Llc Apparatus and method for vapor deposition of dielectric wire coating
WO2012033653A1 (en) 2010-09-10 2012-03-15 New England Biolabs, Inc. A composition, method and kit for obtaining purified recombinant proteins
AU2012236896A1 (en) 2011-03-25 2013-05-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
FR2984328B1 (fr) 2011-12-20 2016-12-30 Bio-Rad Innovations Procede de detection d'une infection par le virus de l'hepatite c
JP6510747B2 (ja) * 2013-08-02 2019-05-08 東洋ビーネット株式会社 プロテアーゼ活性測定法
CA3024331A1 (en) 2016-05-16 2017-11-23 President And Fellows Of Harvard College Method for purification and activation of botulinum neurotoxin
CN111378646A (zh) * 2018-12-27 2020-07-07 北京贝瑞和康生物技术有限公司 一种快速单反应管提取长片段基因组dna的方法和试剂盒
JP2019068869A (ja) * 2019-02-18 2019-05-09 東洋ビーネット株式会社 プロテアーゼ活性測定法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8412517D0 (en) * 1984-05-16 1984-06-20 Nagai K Recombinant fusion proteins
US4569794A (en) * 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins

Also Published As

Publication number Publication date
DK84288A (da) 1988-09-11
JP2686090B2 (ja) 1997-12-08
US5284933A (en) 1994-02-08
EP0282042A3 (de) 1991-09-11
US5310663A (en) 1994-05-10
EP0282042A2 (de) 1988-09-14
EP0282042B1 (de) 1994-06-08
NZ223735A (en) 1990-10-26
DK173951B1 (da) 2002-03-04
IE880685L (en) 1988-09-10
IE63991B1 (en) 1995-06-28
JPS63251095A (ja) 1988-10-18
ZA881534B (en) 1988-09-12
AU1270988A (en) 1988-09-15
CA1340522C (en) 1999-05-04
ATE106897T1 (de) 1994-06-15
DE3889949D1 (de) 1994-07-14
AU609783B2 (en) 1991-05-09

Similar Documents

Publication Publication Date Title
DK84288A (da) Fusionsproteiner og oprensning deraf
DE3779501D1 (de) Neue metallchelatharze.
KR950700779A (ko) 단백질의 정제방법(Protein purification)
DK142194A (da) Fremgangsmåde til affinitetsseparering
DE69232549D1 (de) Austauschbare säulenkartusche für gaschromatographie
ATE393217T1 (de) Verfahren zur reinigung von faktor-ix
DK166546C (da) Fremgangsmaade til spaltning af peptider og proteiner ved methionyl-bindingen
RO95694A (ro) Procedeu pentru recuperarea metalelor neferoase
ATE88718T1 (de) Verfahren zur reinigung des hepatitis-oberflaechen-antigens und das so erhaltene produkt.
DK0789767T3 (da) Fremgangsmåde til fremstilling af oprensede papilomavirusproteiner
DK0428984T3 (da) Fremgangsmåde til fraskillelse af aminosyrer
DE59309127D1 (de) Verfahren zur Reaktivierung von gereinigten Membranproteinen
DE3671247D1 (de) Verfahren zur schmelzreduktion von eisenerzen.
DK436982A (da) Fremgangsmaade til forbedring af kvaliteten af valleprotein
DK447685D0 (da) Fremgangsmaade til fremstilling af mel med hoej proteinkvalitet
EP0316748A3 (en) Process for the selective cleavage of fusion proteins
DK0401657T3 (da) Fremgangsmåde til C-terminal modificering af proteiner
DE3762076D1 (de) Verfahren zum vakuumdichten verbinden zweier metallener gehaeuseteile.
DE68917769D1 (de) Verfahren zur Raffination der Aminosäuren.
DK0415557T3 (da) Separation af antimetalchelat-antistoffer
DK0433978T3 (da) Fremgangsmåde til fremstilling af nojirimycin og beslægtede forbindelser
DK93884D0 (da) Fremgangsmade til fremstilling af 1,5-dibeskyttede-1,5,10-triazadecaner, mellemprodukter derfor samt disses fremstilling
DK77784A (da) Apparat til fremstilling af co2 til mig-svejsere

Legal Events

Date Code Title Description
AHS Application shelved for other reasons than non-payment
B1 Patent granted (law 1993)
PUP Patent expired