ZA202308325B - Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins - Google Patents

Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins

Info

Publication number
ZA202308325B
ZA202308325B ZA2023/08325A ZA202308325A ZA202308325B ZA 202308325 B ZA202308325 B ZA 202308325B ZA 2023/08325 A ZA2023/08325 A ZA 2023/08325A ZA 202308325 A ZA202308325 A ZA 202308325A ZA 202308325 B ZA202308325 B ZA 202308325B
Authority
ZA
South Africa
Prior art keywords
cas
subsequence
grna
genomic library
library preparation
Prior art date
Application number
ZA2023/08325A
Other languages
English (en)
Inventor
Andrew Kennedy
Sarah SHULTZABERGER
Emma Bell
Oliver Miller
Kim Schneider
Esther Musgrave-Brown
Niall Gormley
Andrew Slatter
Feng Chen
Original Assignee
Illumina Inc
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc, Illumina Cambridge Ltd filed Critical Illumina Inc
Publication of ZA202308325B publication Critical patent/ZA202308325B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ZA2023/08325A 2021-03-09 2023-08-29 Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins ZA202308325B (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202163158492P 2021-03-09 2021-03-09
US202163162775P 2021-03-18 2021-03-18
US202163163381P 2021-03-19 2021-03-19
US202163228344P 2021-08-02 2021-08-02
US202163246879P 2021-09-22 2021-09-22
US202163295432P 2021-12-30 2021-12-30
PCT/US2022/019252 WO2022192186A1 (en) 2021-03-09 2022-03-08 Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins

Publications (1)

Publication Number Publication Date
ZA202308325B true ZA202308325B (en) 2024-05-30

Family

ID=80937135

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA2023/08325A ZA202308325B (en) 2021-03-09 2023-08-29 Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins

Country Status (11)

Country Link
US (1) US20240287504A1 (enExample)
EP (1) EP4305163A1 (enExample)
JP (1) JP2024510206A (enExample)
KR (1) KR20230154078A (enExample)
AU (1) AU2022234741A1 (enExample)
BR (1) BR112023018152A2 (enExample)
CA (1) CA3209074A1 (enExample)
IL (1) IL305444B2 (enExample)
MX (1) MX2023010650A (enExample)
WO (1) WO2022192186A1 (enExample)
ZA (1) ZA202308325B (enExample)

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* Cited by examiner, † Cited by third party
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US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
WO1995003430A1 (en) 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
US5773733A (en) 1996-04-12 1998-06-30 National Science Council Alumina-aluminum nitride-nickel composites
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
US6155972A (en) 1999-02-02 2000-12-05 Acorn Cardiovascular, Inc. Cardiac constraint jacket construction
ATE362532T1 (de) 1999-03-19 2007-06-15 Takara Bio Inc Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6326173B1 (en) 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
DK1218542T3 (da) 1999-09-13 2004-08-02 Nugen Technologies Inc Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser
EA005739B1 (ru) 2000-08-23 2005-06-30 Такара Био Инк. Способ амплификации нуклеиновых кислот
US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
WO2004048594A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
JP2010021219A (ja) 2008-07-09 2010-01-28 Nec Schott Components Corp パッケージングデバイス装置およびパッケージ用ベース部材
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Also Published As

Publication number Publication date
IL305444B1 (en) 2024-10-01
US20240287504A1 (en) 2024-08-29
EP4305163A1 (en) 2024-01-17
AU2022234741A1 (en) 2023-09-14
CA3209074A1 (en) 2022-09-15
JP2024510206A (ja) 2024-03-06
KR20230154078A (ko) 2023-11-07
WO2022192186A1 (en) 2022-09-15
MX2023010650A (es) 2023-11-28
BR112023018152A2 (pt) 2023-10-31
IL305444A (en) 2023-10-01
IL305444B2 (en) 2025-02-01

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