JP2024510206A - ＣＡＳ－ｇＲＮＡリボ核タンパク質を使用するゲノムライブラリー調製及び標的化エピジェネティックアッセイ - Google Patents

ＣＡＳ－ｇＲＮＡリボ核タンパク質を使用するゲノムライブラリー調製及び標的化エピジェネティックアッセイ Download PDF

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JP2024510206A
JP2024510206A JP2023555515A JP2023555515A JP2024510206A JP 2024510206 A JP2024510206 A JP 2024510206A JP 2023555515 A JP2023555515 A JP 2023555515A JP 2023555515 A JP2023555515 A JP 2023555515A JP 2024510206 A JP2024510206 A JP 2024510206A
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cas
grna
base pairs
composition
fragment
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JP2024510206A5 (enExample
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ケネディー アンドリュー
シュルツァバージャー サラ
ベル エマ
ミラー オリバー
シュナイダー キム
マスグレイブ‐ブラウン エステル
ゴームリー ニアル
スラッター アンドリュー
チェン フェング
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イルミナ インコーポレイテッド
イルミナ ケンブリッジ リミテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023555515A 2021-03-09 2022-03-08 ＣＡＳ－ｇＲＮＡリボ核タンパク質を使用するゲノムライブラリー調製及び標的化エピジェネティックアッセイ Pending JP2024510206A (ja)

Applications Claiming Priority (13)

Application Number Priority Date Filing Date Title
US202163158492P 2021-03-09 2021-03-09
US63/158,492 2021-03-09
US202163162775P 2021-03-18 2021-03-18
US63/162,775 2021-03-18
US202163163381P 2021-03-19 2021-03-19
US63/163,381 2021-03-19
US202163228344P 2021-08-02 2021-08-02
US63/228,344 2021-08-02
US202163246879P 2021-09-22 2021-09-22
US63/246,879 2021-09-22
US202163295432P 2021-12-30 2021-12-30
US63/295,432 2021-12-30
PCT/US2022/019252 WO2022192186A1 (en) 2021-03-09 2022-03-08 Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins

Publications (2)

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JP2024510206A true JP2024510206A (ja) 2024-03-06
JP2024510206A5 JP2024510206A5 (enExample) 2025-03-19

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US (1) US20240287504A1 (enExample)
EP (1) EP4305163A1 (enExample)
JP (1) JP2024510206A (enExample)
KR (1) KR20230154078A (enExample)
AU (1) AU2022234741A1 (enExample)
BR (1) BR112023018152A2 (enExample)
CA (1) CA3209074A1 (enExample)
IL (1) IL305444B2 (enExample)
MX (1) MX2023010650A (enExample)
WO (1) WO2022192186A1 (enExample)
ZA (1) ZA202308325B (enExample)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018500937A (ja) * 2014-12-20 2018-01-18 アーク バイオ， エルエルシー Ｃｒｉｓｐｒ／ｃａｓ系タンパク質を使用する核酸の標的化枯渇、富化および分割のための組成物および方法

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WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5593826A (en) 1993-03-22 1997-01-14 Perkin-Elmer Corporation, Applied Biosystems, Inc. Enzymatic ligation of 3'amino-substituted oligonucleotides
ES2204913T3 (es) 1993-04-12 2004-05-01 Northwestern University Metodo para formacion de oligonucleotidos.
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
CA2122203C (en) 1993-05-11 2001-12-18 Melinda S. Fraiser Decontamination of nucleic acid amplification reactions
WO1995003430A1 (en) 1993-07-23 1995-02-02 Gen-Probe Incorporated Methods for enhancing nucleic acid amplification
FR2708288B1 (fr) 1993-07-26 1995-09-01 Bio Merieux Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé.
US5523204A (en) 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
SE9400522D0 (sv) 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5677170A (en) 1994-03-02 1997-10-14 The Johns Hopkins University In vitro transposition of artificial transposons
WO1995025180A1 (en) 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
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FR2726277B1 (fr) 1994-10-28 1996-12-27 Bio Merieux Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
CA2249717A1 (en) 1996-03-18 1997-09-25 Richard K. Wilkosz Target nucleic acid sequence amplification
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EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
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ATE362532T1 (de) 1999-03-19 2007-06-15 Takara Bio Inc Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers
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US6927028B2 (en) * 2001-08-31 2005-08-09 Chinese University Of Hong Kong Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA
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JP2018500937A (ja) * 2014-12-20 2018-01-18 アーク バイオ， エルエルシー Ｃｒｉｓｐｒ／ｃａｓ系タンパク質を使用する核酸の標的化枯渇、富化および分割のための組成物および方法

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ZA202308325B (en) 2024-05-30
IL305444B1 (en) 2024-10-01
US20240287504A1 (en) 2024-08-29
EP4305163A1 (en) 2024-01-17
AU2022234741A1 (en) 2023-09-14
CA3209074A1 (en) 2022-09-15
KR20230154078A (ko) 2023-11-07
WO2022192186A1 (en) 2022-09-15
MX2023010650A (es) 2023-11-28
BR112023018152A2 (pt) 2023-10-31
IL305444A (en) 2023-10-01
IL305444B2 (en) 2025-02-01

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