IL305444B2 - Genomic library preparation and targeted epigenetic testing using Cas–Grna ribonucleoproteins - Google Patents
Genomic library preparation and targeted epigenetic testing using Cas–Grna ribonucleoproteinsInfo
- Publication number
- IL305444B2 IL305444B2 IL305444A IL30544423A IL305444B2 IL 305444 B2 IL305444 B2 IL 305444B2 IL 305444 A IL305444 A IL 305444A IL 30544423 A IL30544423 A IL 30544423A IL 305444 B2 IL305444 B2 IL 305444B2
- Authority
- IL
- Israel
- Prior art keywords
- cas
- examples
- double
- grna
- base pairs
- Prior art date
Links
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
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| US202163158492P | 2021-03-09 | 2021-03-09 | |
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| US202163163381P | 2021-03-19 | 2021-03-19 | |
| US202163228344P | 2021-08-02 | 2021-08-02 | |
| US202163246879P | 2021-09-22 | 2021-09-22 | |
| US202163295432P | 2021-12-30 | 2021-12-30 | |
| PCT/US2022/019252 WO2022192186A1 (en) | 2021-03-09 | 2022-03-08 | Genomic library preparation and targeted epigenetic assays using cas-grna ribonucleoproteins |
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Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| WO1994003624A1 (en) | 1992-08-04 | 1994-02-17 | Auerbach Jeffrey I | Methods for the isothermal amplification of nucleic acid molecules |
| US5834202A (en) | 1992-08-04 | 1998-11-10 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US5614389A (en) | 1992-08-04 | 1997-03-25 | Replicon, Inc. | Methods for the isothermal amplification of nucleic acid molecules |
| US6261808B1 (en) | 1992-08-04 | 2001-07-17 | Replicon, Inc. | Amplification of nucleic acid molecules via circular replicons |
| US5593826A (en) | 1993-03-22 | 1997-01-14 | Perkin-Elmer Corporation, Applied Biosystems, Inc. | Enzymatic ligation of 3'amino-substituted oligonucleotides |
| ES2204913T3 (es) | 1993-04-12 | 2004-05-01 | Northwestern University | Metodo para formacion de oligonucleotidos. |
| US5422252A (en) | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
| CA2122203C (en) | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| WO1995003430A1 (en) | 1993-07-23 | 1995-02-02 | Gen-Probe Incorporated | Methods for enhancing nucleic acid amplification |
| FR2708288B1 (fr) | 1993-07-26 | 1995-09-01 | Bio Merieux | Procédé d'amplification d'acides nucléiques par transcription utilisant le déplacement, réactifs et nécessaire pour la mise en Óoeuvre de ce procédé. |
| US5523204A (en) | 1993-12-10 | 1996-06-04 | Becton Dickinson And Company | Detection of nucleic acids in cells by strand displacement amplification |
| SE9400522D0 (sv) | 1994-02-16 | 1994-02-16 | Ulf Landegren | Method and reagent for detecting specific nucleotide sequences |
| US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
| WO1995025180A1 (en) | 1994-03-16 | 1995-09-21 | Gen-Probe Incorporated | Isothermal strand displacement nucleic acid amplification |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| FR2726277B1 (fr) | 1994-10-28 | 1996-12-27 | Bio Merieux | Oligonucleotide utilisable comme amorce dans une methode d'amplification basee sur une replication avec deplacement de brin |
| US5916779A (en) | 1995-09-21 | 1999-06-29 | Becton, Dickinson And Company | Strand displacement amplification of RNA targets |
| US5631147A (en) | 1995-09-21 | 1997-05-20 | Becton, Dickinson And Company | Detection of nucleic acids in cells by thermophilic strand displacement amplification |
| CA2249717A1 (en) | 1996-03-18 | 1997-09-25 | Richard K. Wilkosz | Target nucleic acid sequence amplification |
| US5773733A (en) | 1996-04-12 | 1998-06-30 | National Science Council | Alumina-aluminum nitride-nickel composites |
| US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
| EP1020534B2 (en) | 1998-11-09 | 2011-01-05 | Eiken Kagaku Kabushiki Kaisha | Process for synthesizing nucleic acid |
| US6155972A (en) | 1999-02-02 | 2000-12-05 | Acorn Cardiovascular, Inc. | Cardiac constraint jacket construction |
| ATE362532T1 (de) | 1999-03-19 | 2007-06-15 | Takara Bio Inc | Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers |
| US6238868B1 (en) | 1999-04-12 | 2001-05-29 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology |
| US6326173B1 (en) | 1999-04-12 | 2001-12-04 | Nanogen/Becton Dickinson Partnership | Electronically mediated nucleic acid amplification in NASBA |
| US6309833B1 (en) | 1999-04-12 | 2001-10-30 | Nanogen/Becton Dickinson Partnership | Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures |
| DK1218542T3 (da) | 1999-09-13 | 2004-08-02 | Nugen Technologies Inc | Fremgangsmåder og sammensætninger til lineær isotermisk amplifikation af polynukleotidsekvenser |
| EA005739B1 (ru) | 2000-08-23 | 2005-06-30 | Такара Био Инк. | Способ амплификации нуклеиновых кислот |
| US6927028B2 (en) * | 2001-08-31 | 2005-08-09 | Chinese University Of Hong Kong | Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA |
| WO2004048594A2 (en) | 2002-11-21 | 2004-06-10 | Epicentre Technologies | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| US20050153333A1 (en) | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| JP2010021219A (ja) | 2008-07-09 | 2010-01-28 | Nec Schott Components Corp | パッケージングデバイス装置およびパッケージ用ベース部材 |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| DE102012219142B4 (de) * | 2012-10-19 | 2016-02-25 | Analytik Jena Ag | Verfahren zum Trennen, Erfassen oder Anreichern von unterschiedlichen DNA-Spezies |
| WO2016100955A2 (en) * | 2014-12-20 | 2016-06-23 | Identifygenomics, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
| WO2021252617A1 (en) | 2020-06-09 | 2021-12-16 | Illumina, Inc. | Methods for increasing yield of sequencing libraries |
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| US20240287504A1 (en) | 2024-08-29 |
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| CA3209074A1 (en) | 2022-09-15 |
| JP2024510206A (ja) | 2024-03-06 |
| KR20230154078A (ko) | 2023-11-07 |
| WO2022192186A1 (en) | 2022-09-15 |
| MX2023010650A (es) | 2023-11-28 |
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