ZA200410134B - Method for transforming soybean. - Google Patents
Method for transforming soybean. Download PDFInfo
- Publication number
- ZA200410134B ZA200410134B ZA200410134A ZA200410134A ZA200410134B ZA 200410134 B ZA200410134 B ZA 200410134B ZA 200410134 A ZA200410134 A ZA 200410134A ZA 200410134 A ZA200410134 A ZA 200410134A ZA 200410134 B ZA200410134 B ZA 200410134B
- Authority
- ZA
- South Africa
- Prior art keywords
- soybean
- shoot
- plant
- seed
- primary
- Prior art date
Links
- 244000068988 Glycine max Species 0.000 title claims description 65
- 235000010469 Glycine max Nutrition 0.000 title claims description 55
- 238000000034 method Methods 0.000 title claims description 53
- 230000001131 transforming effect Effects 0.000 title claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 68
- 108090000623 proteins and genes Proteins 0.000 claims description 56
- 241000589158 Agrobacterium Species 0.000 claims description 29
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 23
- 230000009261 transgenic effect Effects 0.000 claims description 17
- 150000007523 nucleic acids Chemical class 0.000 claims description 12
- 108020004707 nucleic acids Proteins 0.000 claims description 9
- 102000039446 nucleic acids Human genes 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 230000001939 inductive effect Effects 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 230000001172 regenerating effect Effects 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 239000003550 marker Substances 0.000 claims description 6
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 claims description 4
- 239000004062 cytokinin Substances 0.000 claims description 4
- 239000005556 hormone Substances 0.000 claims description 4
- 229940088597 hormone Drugs 0.000 claims description 4
- 108090000769 Isomerases Proteins 0.000 claims description 3
- 229930192334 Auxin Natural products 0.000 claims description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 2
- 239000002363 auxin Substances 0.000 claims description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 2
- 229960001669 kinetin Drugs 0.000 claims description 2
- 230000000630 rising effect Effects 0.000 claims description 2
- SKZKKFZAGNVIMN-UHFFFAOYSA-N Salicilamide Chemical compound NC(=O)C1=CC=CC=C1O SKZKKFZAGNVIMN-UHFFFAOYSA-N 0.000 claims 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims 1
- 125000000311 mannosyl group Chemical group C1([C@@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
- 239000002609 medium Substances 0.000 description 55
- 210000004027 cell Anatomy 0.000 description 32
- 210000001519 tissue Anatomy 0.000 description 24
- 229930006000 Sucrose Natural products 0.000 description 21
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 21
- 239000005720 sucrose Substances 0.000 description 21
- 239000013612 plasmid Substances 0.000 description 20
- 230000009466 transformation Effects 0.000 description 17
- 150000003839 salts Chemical class 0.000 description 15
- 229940088594 vitamin Drugs 0.000 description 15
- 239000011782 vitamin Substances 0.000 description 15
- 235000013343 vitamin Nutrition 0.000 description 15
- 229930003231 vitamin Natural products 0.000 description 15
- 230000008929 regeneration Effects 0.000 description 14
- 238000011069 regeneration method Methods 0.000 description 14
- 229920001817 Agar Polymers 0.000 description 13
- 239000008272 agar Substances 0.000 description 13
- 230000014509 gene expression Effects 0.000 description 13
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 11
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 11
- 229960001230 asparagine Drugs 0.000 description 11
- 235000009582 asparagine Nutrition 0.000 description 11
- 230000012010 growth Effects 0.000 description 10
- OHKOGUYZJXTSFX-KZFFXBSXSA-N ticarcillin Chemical compound C=1([C@@H](C(O)=O)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)C=CSC=1 OHKOGUYZJXTSFX-KZFFXBSXSA-N 0.000 description 10
- 229960004659 ticarcillin Drugs 0.000 description 10
- AZZMGZXNTDTSME-JUZDKLSSSA-M cefotaxime sodium Chemical compound [Na+].N([C@@H]1C(N2C(=C(COC(C)=O)CS[C@@H]21)C([O-])=O)=O)C(=O)\C(=N/OC)C1=CSC(N)=N1 AZZMGZXNTDTSME-JUZDKLSSSA-M 0.000 description 9
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 9
- 229960004261 cefotaxime Drugs 0.000 description 8
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 description 8
- 108700019146 Transgenes Proteins 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 101710164820 Flotillin-2 Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 238000001476 gene delivery Methods 0.000 description 6
- 230000001404 mediated effect Effects 0.000 description 6
- 239000002689 soil Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000012882 rooting medium Substances 0.000 description 5
- 238000000844 transformation Methods 0.000 description 5
- 239000013598 vector Substances 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 4
- 101000581817 Rattus norvegicus Regenerating islet-derived protein 3-alpha Proteins 0.000 description 4
- 101710199430 TATA-box-binding protein 2 Proteins 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 108091006047 fluorescent proteins Proteins 0.000 description 4
- 238000005755 formation reaction Methods 0.000 description 4
- 239000004009 herbicide Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 4
- 238000012546 transfer Methods 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 101710199392 TATA-box-binding protein 1 Proteins 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- -1 e=.g. Natural products 0.000 description 3
- 230000002363 herbicidal effect Effects 0.000 description 3
- 101150082349 pmi gene Proteins 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 229960000268 spectinomycin Drugs 0.000 description 3
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
- GOSWTRUMMSCNCW-HNNGNKQASA-N 9-ribosyl-trans-zeatin Chemical compound C1=NC=2C(NC\C=C(CO)/C)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O GOSWTRUMMSCNCW-HNNGNKQASA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 101710164822 Flotillin-1 Proteins 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000257303 Hymenoptera Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 102000004195 Isomerases Human genes 0.000 description 2
- 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- GBXZONVFWYCRPT-KVTDHHQDSA-N [(2s,3s,4r,5r)-3,4,5,6-tetrahydroxy-1-oxohexan-2-yl] dihydrogen phosphate Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](C=O)OP(O)(O)=O GBXZONVFWYCRPT-KVTDHHQDSA-N 0.000 description 2
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 101150103518 bar gene Proteins 0.000 description 2
- 235000013405 beer Nutrition 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 125000004093 cyano group Chemical group *C#N 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- 101150054900 gus gene Proteins 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- 229940064880 inositol 100 mg Drugs 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 229960003512 nicotinic acid Drugs 0.000 description 2
- 235000001968 nicotinic acid Nutrition 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 238000003976 plant breeding Methods 0.000 description 2
- 235000008160 pyridoxine Nutrition 0.000 description 2
- 239000011677 pyridoxine Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 230000011869 shoot development Effects 0.000 description 2
- 229910001961 silver nitrate Inorganic materials 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 230000030118 somatic embryogenesis Effects 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 235000019157 thiamine Nutrition 0.000 description 2
- 229940027257 timentin Drugs 0.000 description 2
- GOSWTRUMMSCNCW-UHFFFAOYSA-N trans-zeatin riboside Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1OC(CO)C(O)C1O GOSWTRUMMSCNCW-UHFFFAOYSA-N 0.000 description 2
- 229940011671 vitamin b6 Drugs 0.000 description 2
- VKWIASMEGJUYIK-UHFFFAOYSA-N 2-pent-1-enyl-7H-purin-6-amine Chemical compound CCCC=Cc1nc(N)c2[nH]cnc2n1 VKWIASMEGJUYIK-UHFFFAOYSA-N 0.000 description 1
- UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 description 1
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 1
- 108091000044 4-hydroxy-tetrahydrodipicolinate synthase Proteins 0.000 description 1
- QUTYKIXIUDQOLK-PRJMDXOYSA-N 5-O-(1-carboxyvinyl)-3-phosphoshikimic acid Chemical compound O[C@H]1[C@H](OC(=C)C(O)=O)CC(C(O)=O)=C[C@H]1OP(O)(O)=O QUTYKIXIUDQOLK-PRJMDXOYSA-N 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 101710103719 Acetolactate synthase large subunit Proteins 0.000 description 1
- 101710182467 Acetolactate synthase large subunit IlvB1 Proteins 0.000 description 1
- 101710171176 Acetolactate synthase large subunit IlvG Proteins 0.000 description 1
- 101710176702 Acetolactate synthase small subunit Proteins 0.000 description 1
- 101710147947 Acetolactate synthase small subunit 1, chloroplastic Proteins 0.000 description 1
- 101710095712 Acetolactate synthase, mitochondrial Proteins 0.000 description 1
- 102000000452 Acetyl-CoA carboxylase Human genes 0.000 description 1
- 108010016219 Acetyl-CoA carboxylase Proteins 0.000 description 1
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241001167018 Aroa Species 0.000 description 1
- XUKUURHRXDUEBC-KAYWLYCHSA-N Atorvastatin Chemical compound C=1C=CC=CC=1C1=C(C=2C=CC(F)=CC=2)N(CC[C@@H](O)C[C@@H](O)CC(O)=O)C(C(C)C)=C1C(=O)NC1=CC=CC=C1 XUKUURHRXDUEBC-KAYWLYCHSA-N 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 108010006654 Bleomycin Proteins 0.000 description 1
- 239000005489 Bromoxynil Substances 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
- 241000725101 Clea Species 0.000 description 1
- 241000518994 Conta Species 0.000 description 1
- 241000111471 Convolvulus scoparius Species 0.000 description 1
- 108030005585 Cyanamide hydratases Proteins 0.000 description 1
- 102100024746 Dihydrofolate reductase Human genes 0.000 description 1
- 101100301524 Drosophila melanogaster Reg-5 gene Proteins 0.000 description 1
- 101100491986 Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) aromA gene Proteins 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 241001640034 Heteropterys Species 0.000 description 1
- DOMWKUIIPQCAJU-LJHIYBGHSA-N Hydroxyprogesterone caproate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(C)=O)(OC(=O)CCCCC)[C@@]1(C)CC2 DOMWKUIIPQCAJU-LJHIYBGHSA-N 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 102000048193 Mannose-6-phosphate isomerases Human genes 0.000 description 1
- 241000252067 Megalops atlanticus Species 0.000 description 1
- 241001214257 Mene Species 0.000 description 1
- 101100238304 Mus musculus Morc1 gene Proteins 0.000 description 1
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 1
- 229930193140 Neomycin Natural products 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 101710196435 Probable acetolactate synthase large subunit Proteins 0.000 description 1
- 101710181764 Probable acetolactate synthase small subunit Proteins 0.000 description 1
- 101710104000 Putative acetolactate synthase small subunit Proteins 0.000 description 1
- 102100037889 Regenerating islet-derived protein 4 Human genes 0.000 description 1
- 101710193245 Regenerating islet-derived protein 4 Proteins 0.000 description 1
- MJNIWUJSIGSWKK-UHFFFAOYSA-N Riboflavine 2',3',4',5'-tetrabutanoate Chemical compound CCCC(=O)OCC(OC(=O)CCC)C(OC(=O)CCC)C(OC(=O)CCC)CN1C2=CC(C)=C(C)C=C2N=C2C1=NC(=O)NC2=O MJNIWUJSIGSWKK-UHFFFAOYSA-N 0.000 description 1
- 229940100389 Sulfonylurea Drugs 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- 239000006035 Tryptophane Substances 0.000 description 1
- 101150049278 US20 gene Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 101150037081 aroA gene Proteins 0.000 description 1
- 229940009098 aspartate Drugs 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000008238 biochemical pathway Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229960001561 bleomycin Drugs 0.000 description 1
- OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- CETPSERCERDGAM-UHFFFAOYSA-N ceric oxide Chemical compound O=[Ce]=O CETPSERCERDGAM-UHFFFAOYSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 108020001096 dihydrofolate reductase Proteins 0.000 description 1
- 102000034287 fluorescent proteins Human genes 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
- 150000002333 glycines Chemical class 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000001976 improved effect Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000000749 insecticidal effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000005087 leaf formation Effects 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 229960000485 methotrexate Drugs 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 description 1
- 229960004927 neomycin Drugs 0.000 description 1
- 231100001222 nononcogenic Toxicity 0.000 description 1
- 101150113864 pat gene Proteins 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 235000010204 pine bark Nutrition 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 101150075980 psbA gene Proteins 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 229940124530 sulfonamide Drugs 0.000 description 1
- 150000003456 sulfonamides Chemical class 0.000 description 1
- YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical compound OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 150000003544 thiamines Chemical class 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 229960004799 tryptophan Drugs 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US39056202P | 2002-06-22 | 2002-06-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA200410134B true ZA200410134B (en) | 2005-11-22 |
Family
ID=30000570
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA200410134A ZA200410134B (en) | 2002-06-22 | 2004-12-15 | Method for transforming soybean. |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US20040034889A1 (fr) |
| EP (1) | EP1517991A4 (fr) |
| JP (1) | JP2005535312A (fr) |
| CN (1) | CN1668744A (fr) |
| AR (1) | AR039721A1 (fr) |
| AU (1) | AU2003279185B2 (fr) |
| BR (1) | BR0312005A (fr) |
| CA (1) | CA2490154A1 (fr) |
| NZ (1) | NZ537237A (fr) |
| WO (1) | WO2004000006A2 (fr) |
| ZA (1) | ZA200410134B (fr) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7560611B2 (en) | 2003-08-05 | 2009-07-14 | Monsanto Technology Llc | Method and apparatus for substantially isolating plant tissues |
| US7150993B2 (en) | 2003-08-05 | 2006-12-19 | Monsanto Technology Llc | Method for excision of plant embryos for transformation |
| US8058514B2 (en) * | 2007-02-28 | 2011-11-15 | Japan Tobacco Inc. | Agrobacterium-mediated method for producing transformed plant without selection step |
| US8044260B2 (en) | 2007-03-09 | 2011-10-25 | Monsanto Technology Llc | Method of meristem excision and transformation |
| US20080229447A1 (en) * | 2007-03-12 | 2008-09-18 | Syngenta Participations Ag | Transformation of immature soybean seeds through organogenesis |
| JP2008259497A (ja) * | 2007-03-16 | 2008-10-30 | Takano Foods Kk | アグロバクテリウムを介したダイズ国内品種の形質転換体作出方法並びに形質転換体当代及び後代の種子を短期間で獲得する方法 |
| US20090023212A1 (en) * | 2007-07-19 | 2009-01-22 | Syngenta Participations Ag | Method for transforming soybean (Glycine max) |
| US20090023213A1 (en) * | 2007-07-19 | 2009-01-22 | Syngenta Participations Ag | Method to increase the rate of transgenic embryo formation after inoculation of immature cotyledons with agrobacterium |
| WO2010071608A1 (fr) * | 2008-12-15 | 2010-06-24 | Temasek Life Sciences Laboratory Limited | Transformation génétique de jatropha curcas |
| ES2727575T3 (es) | 2009-01-22 | 2019-10-17 | Syngenta Participations Ag | Polipéptidos de Hidroxifenilpiruvato Dioxigenasa mutantes, y Métodos de uso |
| US9175305B2 (en) | 2009-01-22 | 2015-11-03 | Syngenta Participations Ag | Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
| US9347046B2 (en) * | 2009-01-22 | 2016-05-24 | Syngenta Participations Ag | Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
| CN101717786B (zh) * | 2009-12-11 | 2012-02-08 | 南开大学 | 一种快速获得大豆转基因愈伤组织的转化方法 |
| TWI667347B (zh) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法 |
| CN102181479B (zh) * | 2011-03-14 | 2013-05-15 | 东北农业大学 | 一种农杆菌介导的大豆转基因方法 |
| CN102329816B (zh) * | 2011-06-15 | 2012-12-26 | 北京未名凯拓作物设计中心有限公司 | 一种农杆菌介导的纵切幼苗上胚轴顶端转化大豆的方法 |
| AU2012284205B2 (en) | 2011-07-15 | 2016-11-17 | Syngenta Participations Ag | Methods of increasing yield and stress tolerance in a plant |
| WO2013014585A1 (fr) | 2011-07-22 | 2013-01-31 | Basf Plant Science Company Gmbh | Procédé de transformation de plante |
| CN102499075A (zh) * | 2011-10-09 | 2012-06-20 | 华南农业大学 | 一种制备大豆复合型外植体的方法及用该外植体制备快速转基因大豆植株的方法 |
| AU2013361491A1 (en) * | 2012-12-19 | 2015-06-04 | Dow Agrosciences Llc | Improved soybean transformation for efficient and high-throughput transgenic event production |
| US20170298372A1 (en) * | 2014-09-19 | 2017-10-19 | E I Du Pont De Nemours And Company | Soybean if5a promoter and its use in constitutive expression of transgenic genes in plants |
| CN108265075A (zh) * | 2016-12-29 | 2018-07-10 | 南京农业大学 | 一种提高大豆组培丛生芽伸长率和遗传转化效率的方法 |
| AR113761A1 (es) | 2017-10-18 | 2020-06-10 | Syngenta Participations Ag | Control de plagas de hemípteros utilizando moléculas de arn |
| US11453885B1 (en) * | 2019-02-19 | 2022-09-27 | Inari Agriculture Technology, Inc. | Plant transformation |
| CN110358788B (zh) * | 2019-06-25 | 2020-07-21 | 中国农业科学院作物科学研究所 | 一种以pmi为筛选基因的大豆遗传转化方法 |
| CN115605082A (zh) * | 2019-11-26 | 2023-01-13 | 先正达农作物保护股份公司（Ch） | 转化方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6664109B2 (en) * | 1985-01-17 | 2003-12-16 | Calgene Llc | Transformation system with Ti or Ri plasmid |
| ATE105585T1 (de) * | 1987-12-21 | 1994-05-15 | Univ Toledo | Transformation von keimenden pflanzensamen mit hilfe von agrobacterium. |
| US5416011A (en) * | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| BR9306802A (pt) * | 1992-07-27 | 1998-12-08 | Pioneer Hi Bred Int | Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais |
| GB9307408D0 (en) * | 1993-04-08 | 1993-06-02 | Danisco | Transgenic plants |
| US5959179A (en) * | 1996-03-13 | 1999-09-28 | Monsanto Company | Method for transforming soybeans |
| US5968830A (en) * | 1997-03-28 | 1999-10-19 | Mississippi State University | Soybean transformation and regeneration methods |
| AU6240899A (en) * | 1998-08-31 | 2000-03-21 | Monsanto Technology Llc | Transgene assay using stable agrobacterium rhizogenes transformation |
| CA2359868A1 (fr) * | 1999-01-14 | 2000-07-20 | Monsanto Company | Procede de transformation de soja |
| BR0016367B1 (pt) * | 1999-12-15 | 2013-09-03 | mÉtodo para transformar tecido de explante de soja | |
| US6644777B2 (en) * | 2000-03-29 | 2003-11-11 | Canon Kabushiki Kaisha | Liquid ejecting apparatus, and method for maintaining and recovering ejection performance of the same |
| JP2001278875A (ja) * | 2000-03-31 | 2001-10-10 | Daikin Ind Ltd | ３−フルオロアルコキシメチル−３−アルキルオキセタンの製法 |
| BR0113156A (pt) * | 2000-08-11 | 2003-07-08 | Syngenta Participations Ag | Método para produzir um planta dicotiledÈnea transformada, célula de planta transformada, cultura de múltiplos brotos, planta transformada, semente produzida pela planta transformada, método para produzir uma planta compreendendo um genoma de plastìdeo transformado, genoma de plastìdeo transformado, e, plastìdeo |
| US20030046733A1 (en) * | 2001-09-06 | 2003-03-06 | Dias Kalyani Mallika | Transformation of soybeans |
-
2003
- 2003-06-17 CA CA002490154A patent/CA2490154A1/fr not_active Abandoned
- 2003-06-17 JP JP2004515886A patent/JP2005535312A/ja active Pending
- 2003-06-17 BR BR0312005-8A patent/BR0312005A/pt not_active IP Right Cessation
- 2003-06-17 CN CN03816844.8A patent/CN1668744A/zh active Pending
- 2003-06-17 EP EP03742051A patent/EP1517991A4/fr not_active Withdrawn
- 2003-06-17 US US10/463,074 patent/US20040034889A1/en not_active Abandoned
- 2003-06-17 WO PCT/US2003/019212 patent/WO2004000006A2/fr active Application Filing
- 2003-06-17 US US10/517,039 patent/US20060260012A1/en not_active Abandoned
- 2003-06-17 AU AU2003279185A patent/AU2003279185B2/en not_active Ceased
- 2003-06-17 NZ NZ537237A patent/NZ537237A/en unknown
- 2003-06-20 AR ARP030102206A patent/AR039721A1/es unknown
-
2004
- 2004-12-15 ZA ZA200410134A patent/ZA200410134B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20060260012A1 (en) | 2006-11-16 |
| AU2003279185A1 (en) | 2004-01-06 |
| NZ537237A (en) | 2008-01-31 |
| WO2004000006A2 (fr) | 2003-12-31 |
| CA2490154A1 (fr) | 2003-12-31 |
| AU2003279185B2 (en) | 2008-05-29 |
| JP2005535312A (ja) | 2005-11-24 |
| US20040034889A1 (en) | 2004-02-19 |
| CN1668744A (zh) | 2005-09-14 |
| BR0312005A (pt) | 2005-08-16 |
| EP1517991A2 (fr) | 2005-03-30 |
| WO2004000006A3 (fr) | 2004-05-13 |
| AR039721A1 (es) | 2005-03-09 |
| EP1517991A4 (fr) | 2006-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ZA200410134B (en) | Method for transforming soybean. | |
| Sriskandarajah et al. | Genetic transformation of the apple scion cultivar ‘Delicious’ via Agrobacterium tumefaciens | |
| AU736027B2 (en) | Method for transforming indica rice | |
| US5024944A (en) | Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species | |
| US7910803B2 (en) | Transformation in Camelina sativa | |
| JP2004506427A (ja) | 植物の安定した形質転換の方法 | |
| BRPI0716225B1 (pt) | Methods for the rapid production of a transgenic monocotyledonous plant for the expansion of a development priority window for the production of transgenic plants and for the screening of cells with respect to the transformation capacity | |
| US5792927A (en) | Genetically transformed rose plants and methods for their production | |
| US7960614B2 (en) | Plant transformation and selection | |
| US6255559B1 (en) | Methods for producing genetically modified plants, genetically modified plants, plant materials and plant products produced thereby | |
| Carneiro | Coffee biotechnology and its application in genetic transformation | |
| WO1996025504A1 (fr) | Modification genetique de vegetaux | |
| CN108588002B (zh) | 获得谷子用于遗传转化的胚性愈伤组织和遗传转化的方法 | |
| US20010007157A1 (en) | Genetically transformed rose plants and methods for their production | |
| Joung et al. | Plant transformation methods and applications | |
| O’Kennedy et al. | Improved regeneration efficiency of a pearl millet (Pennisetum glaucum [L.] R. Br.) breeding line | |
| Mangena | A simplified in-planta genetic transformation in soybean | |
| EP1180927B1 (fr) | Transformation d'especes allium a l'aide d'agrobacterium en utilisant des cultures de cals embryogenes | |
| Ahmed | Studies on the tissue culture and potential for the development of a genetic transformation system for avocados (Persea americana Mill.) | |
| US7893323B2 (en) | Transformation of Allium sp. with agrobacterium using embryogenic callus cultures | |
| US20100248372A1 (en) | Composition and Method for Modulating Plant Transformation | |
| US20100251415A1 (en) | Composition and Method for Modulating Plant Transformation | |
| Yakandawala et al. | Transient expression of uida reporter gene in regenerable callus tissues of Anthurium andraeanum lind. by agrobacterium mediated transformation | |
| Khaliluev et al. | Development of regeneration system and study of the transformation potential of a commercial tomato variety | |
| NOMURA | An Efficient Transfornlation System in Chrysanthemum [Dendranthema X grandiflorum (Ramat.) Kitamura Jfor Stable and Noll-chimeric~ l |