AR039721A1 - Metodo para transformar la soja - Google Patents

Metodo para transformar la soja

Info

Publication number
AR039721A1
AR039721A1 ARP030102206A ARP030102206A AR039721A1 AR 039721 A1 AR039721 A1 AR 039721A1 AR P030102206 A ARP030102206 A AR P030102206A AR P030102206 A ARP030102206 A AR P030102206A AR 039721 A1 AR039721 A1 AR 039721A1
Authority
AR
Argentina
Prior art keywords
tissue
cells
soybean
soy
transgenic
Prior art date
Application number
ARP030102206A
Other languages
English (en)
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR039721A1 publication Critical patent/AR039721A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente provee métodos para la transformación medida por Agrobacterium de células o tejido de soja y la regeneración de las células o tejido transformados en plantas transformadas. Los métodos pueden ser utilizados para transformar muchos cultivares de soja. Reivindicación 1: Un método para transformar las células o tejido de soja, que comprende: (a) preparar una explanta a partir de una semilla de soja mediante: (i) la eliminación de un hipocótilo de dicha semilla de soja; (ii) la eliminación de un cotiledón junto con su yema axilar adyacente, dejando las hojas primarias unidas un cotiledón remanente; y (iii) la eliminación de una porción de una hoja primaria de dicho cotiledón remanente, generando de este modo una base de hoja primaria; y (b) co-cultivar dicha explanta con Agrobacterium que comprende al menos un ácido nucleico de interés a ser incorporado en un genoma de una o más células de soja. Reivindicación 19: Una planta de soja transgénica regenerada a partir de células o tejido de soja transformado de acuerdo con el método de la reivindicación 1. Reivindicación 20: Una semilla transgénica producida por la planta transgénica de la reivindicación 19.
ARP030102206A 2002-06-22 2003-06-20 Metodo para transformar la soja AR039721A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US39056202P 2002-06-22 2002-06-22

Publications (1)

Publication Number Publication Date
AR039721A1 true AR039721A1 (es) 2005-03-09

Family

ID=30000570

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP030102206A AR039721A1 (es) 2002-06-22 2003-06-20 Metodo para transformar la soja

Country Status (11)

Country Link
US (2) US20040034889A1 (es)
EP (1) EP1517991A4 (es)
JP (1) JP2005535312A (es)
CN (1) CN1668744A (es)
AR (1) AR039721A1 (es)
AU (1) AU2003279185B2 (es)
BR (1) BR0312005A (es)
CA (1) CA2490154A1 (es)
NZ (1) NZ537237A (es)
WO (1) WO2004000006A2 (es)
ZA (1) ZA200410134B (es)

Families Citing this family (26)

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Publication number Priority date Publication date Assignee Title
US7560611B2 (en) 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US8058514B2 (en) * 2007-02-28 2011-11-15 Japan Tobacco Inc. Agrobacterium-mediated method for producing transformed plant without selection step
US8030544B2 (en) 2007-03-09 2011-10-04 Monsanto Technology Llc Methods for plant transformation using spectinomycin selection
US20080229447A1 (en) * 2007-03-12 2008-09-18 Syngenta Participations Ag Transformation of immature soybean seeds through organogenesis
JP2008259497A (ja) * 2007-03-16 2008-10-30 Takano Foods Kk アグロバクテリウムを介したダイズ国内品種の形質転換体作出方法並びに形質転換体当代及び後代の種子を短期間で獲得する方法
US20090023212A1 (en) * 2007-07-19 2009-01-22 Syngenta Participations Ag Method for transforming soybean (Glycine max)
US20090023213A1 (en) * 2007-07-19 2009-01-22 Syngenta Participations Ag Method to increase the rate of transgenic embryo formation after inoculation of immature cotyledons with agrobacterium
AP3489A (en) * 2008-12-15 2015-12-31 Temasek Life Sciences Lab Ltd Genetic transformation of jatrophy curcas
US9175305B2 (en) 2009-01-22 2015-11-03 Syngenta Participations Ag Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
WO2010085705A2 (en) 2009-01-22 2010-07-29 Syngenta Participations Ag Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
AU2010326672A1 (en) * 2009-07-10 2011-08-04 Syngenta Participations Ag Novel hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
CN101717786B (zh) * 2009-12-11 2012-02-08 南开大学 一种快速获得大豆转基因愈伤组织的转化方法
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
CN102181479B (zh) * 2011-03-14 2013-05-15 东北农业大学 一种农杆菌介导的大豆转基因方法
CN102329816B (zh) * 2011-06-15 2012-12-26 北京未名凯拓作物设计中心有限公司 一种农杆菌介导的纵切幼苗上胚轴顶端转化大豆的方法
AU2012284205B2 (en) 2011-07-15 2016-11-17 Syngenta Participations Ag Methods of increasing yield and stress tolerance in a plant
EP2734035A4 (en) 2011-07-22 2015-03-25 Basf Plant Science Co Gmbh PLANT CONVERSION METHOD
CN102499075A (zh) * 2011-10-09 2012-06-20 华南农业大学 一种制备大豆复合型外植体的方法及用该外植体制备快速转基因大豆植株的方法
JP2016502853A (ja) * 2012-12-19 2016-02-01 ダウ アグロサイエンシィズ エルエルシー 効率的なハイスループットトランスジェニック事象生成のための改善されたダイズ形質転換
US20170298372A1 (en) * 2014-09-19 2017-10-19 E I Du Pont De Nemours And Company Soybean if5a promoter and its use in constitutive expression of transgenic genes in plants
CN108265075A (zh) * 2016-12-29 2018-07-10 南京农业大学 一种提高大豆组培丛生芽伸长率和遗传转化效率的方法
AR113761A1 (es) 2017-10-18 2020-06-10 Syngenta Participations Ag Control de plagas de hemípteros utilizando moléculas de arn
US11453885B1 (en) * 2019-02-19 2022-09-27 Inari Agriculture Technology, Inc. Plant transformation
CN110358788B (zh) * 2019-06-25 2020-07-21 中国农业科学院作物科学研究所 一种以pmi为筛选基因的大豆遗传转化方法
CN115605082A (zh) * 2019-11-26 2023-01-13 先正达农作物保护股份公司（Ch） 转化方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6664109B2 (en) * 1985-01-17 2003-12-16 Calgene Llc Transformation system with Ti or Ri plasmid
DE3889546T2 (de) * 1987-12-21 1994-09-08 Univ Toledo Transformation von keimenden pflanzensamen mit hilfe von agrobacterium.
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
AU670316B2 (en) * 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
GB9307408D0 (en) * 1993-04-08 1993-06-02 Danisco Transgenic plants
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
US5968830A (en) * 1997-03-28 1999-10-19 Mississippi State University Soybean transformation and regeneration methods
US6544733B1 (en) * 1998-08-31 2003-04-08 Monsanto Technology Llc Method of identifying non-host plant disease resistance genes
WO2000042207A2 (en) * 1999-01-14 2000-07-20 Monsanto Technology Llc Soybean transformation method
CA2394367C (en) * 1999-12-15 2014-01-21 Regents Of The University Of Minnesota Method to enhance agrobacterium-mediated transformation of plants
US6644777B2 (en) * 2000-03-29 2003-11-11 Canon Kabushiki Kaisha Liquid ejecting apparatus, and method for maintaining and recovering ejection performance of the same
JP2001278875A (ja) * 2000-03-31 2001-10-10 Daikin Ind Ltd ３−フルオロアルコキシメチル−３−アルキルオキセタンの製法
CA2418056C (en) * 2000-08-11 2012-10-16 Syngenta Participations Ag Methods for stable transformation of plants
US20030046733A1 (en) * 2001-09-06 2003-03-06 Dias Kalyani Mallika Transformation of soybeans

Also Published As

Publication number Publication date
US20040034889A1 (en) 2004-02-19
WO2004000006A3 (en) 2004-05-13
WO2004000006A2 (en) 2003-12-31
BR0312005A (pt) 2005-08-16
US20060260012A1 (en) 2006-11-16
EP1517991A2 (en) 2005-03-30
JP2005535312A (ja) 2005-11-24
EP1517991A4 (en) 2006-03-29
AU2003279185B2 (en) 2008-05-29
CA2490154A1 (en) 2003-12-31
ZA200410134B (en) 2005-11-22
AU2003279185A1 (en) 2004-01-06
NZ537237A (en) 2008-01-31
CN1668744A (zh) 2005-09-14

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