AR049204A1 - Transformacion de porotos de soja - Google Patents

Transformacion de porotos de soja

Info

Publication number
AR049204A1
AR049204A1 ARP050102309A ARP050102309A AR049204A1 AR 049204 A1 AR049204 A1 AR 049204A1 AR P050102309 A ARP050102309 A AR P050102309A AR P050102309 A ARP050102309 A AR P050102309A AR 049204 A1 AR049204 A1 AR 049204A1
Authority
AR
Argentina
Prior art keywords
plant
shoots
tissue
optionally
axillary
Prior art date
Application number
ARP050102309A
Other languages
English (en)
Inventor
P Olhoft
L Grist
L Bernal
S Price
D Arias
H Haiping
Original Assignee
Basf Plant Science Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh filed Critical Basf Plant Science Gmbh
Publication of AR049204A1 publication Critical patent/AR049204A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Métodos mejorados para la incorporacion de ADN en el genoma de una planta de poroto de soja (Glycine max) utilizando células meristemáticas de nudos de hojas primarias o superiores como tejido blanco mediante transformacion mediada por Agrobacterium y posterior regeneracion de las células transformadas a una planta entera. Reivindicacion 1: Un método para producir una planta transgénica de poroto de soja que compende los pasos de: (a) proveer un tejido meristemático axilar de un nudo de hoja primario o superior de una semilla germinada de poroto de soja, y (b) co-cultivar dicho tejido meristemático axilar con un Agrobacterium que comprende un T-ADN transgénico, en donde dicho T-ADN transgénico que comprende al menos un casete de expresion vegetal para un rasgo agronomicamente valiosos, y -opcionalmente- uno o más genes marcadores seleccionables, y (c) transferir dicho tejido meristemático axilar co-cultivado en un medio de induccion de brotes que comprende: (i) al menos un factor de crecimiento vegetal en una concentracion adecuada para inducir la induccion de novo de brotes a partir de dicho tejido meristemático axilar, y (ii) opcionalmente, uno o más de los compuestos de seleccion, que en combinacion con el gen marcador seleccionable de (b) permite la identificacion y/o la seleccion de una célula vegetal, tejido o planta que comprende dicho gen marcador seleccionable, y/o (iii) opcionalmente uno mo más antibioticos adecuados para inhibir el crecimiento de Agrobacterium, y cultivar dicho tejido meristemático axilar co-cultivado hasta que los brotes son inducidos y desarrollan de allí y aislar dichos brotes, y (d) transferir dichos brotes aislados a un medio formador de raíces y cultivar dichos brotes en dicho medio formador de raíces hasta que dichos brotes hayan formado raíces, y luego regenerar los plantines así derivados a plantas maduras, que comprende inssertar en su genoma un T-ADN que comprende dicho al menos un casete de expresion vegetal para un rasgo agronomicamente valioso, y -opcionalmente- dicho al menos un gen marcador seleccionable.
ARP050102309A 2004-06-07 2005-06-07 Transformacion de porotos de soja AR049204A1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US57770804P 2004-06-07 2004-06-07
US62170204P 2004-10-25 2004-10-25
US62913804P 2004-11-18 2004-11-18

Publications (1)

Publication Number Publication Date
AR049204A1 true AR049204A1 (es) 2006-07-05

Family

ID=34969247

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP050102309A AR049204A1 (es) 2004-06-07 2005-06-07 Transformacion de porotos de soja

Country Status (13)

Country Link
US (1) US8541653B2 (es)
EP (1) EP1756282B1 (es)
JP (1) JP4744512B2 (es)
AR (1) AR049204A1 (es)
AT (1) ATE427995T1 (es)
AU (1) AU2005252338B2 (es)
BR (1) BRPI0511857A (es)
CA (1) CA2567779A1 (es)
DE (1) DE602005013775D1 (es)
IL (1) IL179298A (es)
MX (1) MXPA06013357A (es)
RU (1) RU2389797C2 (es)
WO (1) WO2005121345A1 (es)

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GB2437281A (en) * 2006-04-21 2007-10-24 Basf Plant Science Gmbh Linum transformation method using acetohydroxyacid synthase gene selection marker
CN101652480A (zh) 2007-03-23 2010-02-17 巴斯福植物科学有限公司 具有提高的胁迫耐受性和产量的转基因植物
CA2687320A1 (en) 2007-05-29 2008-12-04 Basf Plant Science Gmbh Transgenic plants with increased stress tolerance and yield
EP2520656A3 (en) 2007-07-13 2013-05-01 BASF Plant Science GmbH Transgenic plants with increased stress tolerance and yield
AR067795A1 (es) 2007-08-02 2009-10-21 Basf Plant Science Gmbh Plantas transgenicas con mayor tolerancia al estres y mayor rendimiento
EP2220240A2 (en) 2007-11-27 2010-08-25 BASF Plant Science GmbH Transgenic plants with increased stress tolerance and yield
CA2748973A1 (en) 2009-01-07 2010-07-15 Basf Agrochemical Products B.V. Soybean event 127 and methods related thereto
US8884101B2 (en) 2009-07-29 2014-11-11 Japan Tobacco Inc. Method of gene introduction into Triticum plant using Agrobacterium, and a method of producing transformed Triticum plant
RU2012112347A (ru) 2009-08-31 2013-10-10 Басф Плант Сайенс Компани Гмбх Регуляторные молекулы нуклеиновых кислот для усиления конститутивной генной экспрессии в растениях
RU2559534C2 (ru) 2009-08-31 2015-08-10 Басф Плант Сайенс Компани Гмбх Регуляторные молекулы нуклеиновых кислот для усиления семя-специфичной и/или семя-предпочтительной генной экспрессии в растениях
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DE112011101566T5 (de) 2010-05-04 2013-05-08 Basf Se Pflanzen mit erhöhter herbizidtoleranz
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US9068191B2 (en) 2010-12-29 2015-06-30 Syngenta Participations Ag Methods and compositions for a soybean in-planta transient expression system
EP3434781A3 (en) 2011-03-18 2019-03-06 Basf Plant Science Company GmbH Promoters for regulating expression in plants
JP6076974B2 (ja) * 2011-07-13 2017-02-08 ダウ アグロサイエンシィズ エルエルシー 積み重ねられた除草剤耐性イベント８２６４．４２．３２．１、関連する形質転換大豆株、およびその検出
WO2013014585A1 (en) 2011-07-22 2013-01-31 Basf Plant Science Company Gmbh Plant transformation method
BR102012019434B1 (pt) * 2011-07-26 2021-11-09 Dow Agrosciences Llc Métodos de controle de pestes, de insetos, molécula e sequência de dna diagnóstica para o evento de soja 9582.814.19.1
WO2013092275A2 (en) 2011-12-23 2013-06-27 Basf Plant Science Company Gmbh Genes to enhance the defense against pathogens in plants
US10041087B2 (en) 2012-06-19 2018-08-07 BASF Agro B.V. Plants having increased tolerance to herbicides
AR091489A1 (es) 2012-06-19 2015-02-11 Basf Se Plantas que tienen una mayor tolerancia a herbicidas inhibidores de la protoporfirinogeno oxidasa (ppo)
US10294488B2 (en) 2012-12-18 2019-05-21 Basf Se Herbicide-metabolizing cytochrome P450 monooxygenases
US10462994B2 (en) 2013-01-29 2019-11-05 Basf Plant Science Company Gmbh Fungal resistant plants expressing HCP7
US10231397B2 (en) 2013-01-29 2019-03-19 Basf Plant Science Company Gmbh Fungal resistant plants expressing EIN2
WO2014117990A1 (en) 2013-01-29 2014-08-07 Basf Plant Science Company Gmbh Fungal resistant plants expressing hcp6
US10465204B2 (en) 2013-03-08 2019-11-05 Basf Plant Science Company Gmbh Fungal resistant plants expressing MybTF
US10619138B2 (en) 2013-08-12 2020-04-14 Basf Se Herbicide-resistant hydroxyphenylpyruvate dioxygenases
EA201690374A1 (ru) 2013-08-12 2016-07-29 Басф Агро Б.В. Растения с повышенной устойчивостью к гербицидам
BR112016002835A2 (pt) 2013-08-12 2017-09-19 Basf Agro Bv Plantas, semente, células vegetais, produtos vegetais, prole ou planta descendente derivada de uma planta, método de controle de ervas, método de produção de planta, método de produção de plantas de prole, método de produção de produtos vegetais, método de cultivo da planta, combinação útil para controle de ervas, processos de preparação da combinação útil para controle de ervas e uso da combinação
AU2014315191A1 (en) 2013-09-04 2016-04-21 Indigo Ag, Inc. Agricultural endophyte-plant compositions, and methods of use
WO2015150465A2 (en) 2014-04-03 2015-10-08 Basf Se Plants having increased tolerance to herbicides
EP3140811A4 (en) 2014-05-06 2018-01-24 Dow Agrosciences LLC System for imaging and orienting seeds and method of use
US9980424B2 (en) 2014-05-06 2018-05-29 Dow Agrosciences Llc System for seed preparation and method of use
US20170137833A1 (en) * 2014-05-16 2017-05-18 Kaneka Corporation Agrobacterium and method for producing transformed plant using the same
WO2016124515A1 (en) 2015-02-04 2016-08-11 Basf Plant Science Company Gmbh Method of increasing resistance against soybean rust in transgenic plants by increasing the scopoletin content
WO2016128470A1 (en) 2015-02-11 2016-08-18 Basf Se Herbicide-resistant hydroxyphenylpyruvate dioxygenases
EP3054014A3 (en) 2016-05-10 2016-11-23 BASF Plant Science Company GmbH Use of a fungicide on transgenic plants
BR112019001483A2 (pt) 2016-07-27 2019-09-10 Basf Agro Bv método para controlar a vegetação indesejada em um local de cultivo de plantas
WO2018114759A1 (en) 2016-12-20 2018-06-28 BASF Agro B.V. Plants having increased tolerance to herbicides
EA202091289A1 (ru) 2017-11-29 2020-10-12 Басф Се Растения, обладающие повышенной устойчивостью к гербицидам
EP3508581A1 (en) 2018-01-03 2019-07-10 Kws Saat Se Regeneration of genetically modified plants
BR112021017973A2 (pt) 2019-03-12 2021-11-16 Basf Se Célula, construção de expressão, planta ou parte de planta, conjunto de plantas, semente, acúmulo de sementes, uso de uma planta, métodos para criar uma célula, para produzir uma população de plantas, para reduzir ou abolir a susceptibilidade a infecções e métodos de ensaio de uma planta e de propagação de uma planta
CN114945273A (zh) 2019-11-29 2022-08-26 巴斯夫欧洲公司 增加植物对抗真菌感染的抗性
WO2023031161A1 (en) 2021-09-03 2023-03-09 BASF Agricultural Solutions Seed US LLC Plants having increased tolerance to herbicides
WO2023041649A1 (en) 2021-09-20 2023-03-23 Basf Plant Science Company Gmbh Coumarin transporters and uses thereof
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Also Published As

Publication number Publication date
CA2567779A1 (en) 2005-12-22
EP1756282A1 (en) 2007-02-28
EP1756282B1 (en) 2009-04-08
DE602005013775D1 (de) 2009-05-20
AU2005252338A1 (en) 2005-12-22
IL179298A (en) 2010-12-30
RU2006146885A (ru) 2008-07-20
JP2008501327A (ja) 2008-01-24
BRPI0511857A (pt) 2008-01-22
ATE427995T1 (de) 2009-04-15
US8541653B2 (en) 2013-09-24
RU2389797C2 (ru) 2010-05-20
JP4744512B2 (ja) 2011-08-10
WO2005121345A1 (en) 2005-12-22
IL179298A0 (en) 2007-03-08
AU2005252338B2 (en) 2008-12-18
US20090049567A1 (en) 2009-02-19
MXPA06013357A (es) 2007-03-01

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