AR068470A1 - Metodo para producir una planta transgenica a partir del contacto de un explante con una construccion genetica - Google Patents

Metodo para producir una planta transgenica a partir del contacto de un explante con una construccion genetica

Info

Publication number
AR068470A1
AR068470A1 ARP080104043A ARP080104043A AR068470A1 AR 068470 A1 AR068470 A1 AR 068470A1 AR P080104043 A ARP080104043 A AR P080104043A AR P080104043 A ARP080104043 A AR P080104043A AR 068470 A1 AR068470 A1 AR 068470A1
Authority
AR
Argentina
Prior art keywords
methods
bud
explant
contact
cells
Prior art date
Application number
ARP080104043A
Other languages
English (en)
Inventor
Qiudeng Que
John Dawson
Marina Sigareva
Original Assignee
Syngenta Participations Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag filed Critical Syngenta Participations Ag
Publication of AR068470A1 publication Critical patent/AR068470A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

Abstract

Métodos para la transformacion de células o tejido de soja y la regeneracion de las células o tejido de soja en plantas transformadas. Los métodos que se divulgan utilizan un explante preparado a partir de una plántula de soja inmadura que se puede inducir directamente a partir de brotes que dan origen a plantas transgénicas por organogénesis. Dichos métodos seleccionan entre diferentes explantes a las semillas de soja inmaduras en las etapas de desarrollo R5 tardía, R6 o R7. Las construcciones genéticas que se ponen en contacto con el explante comprenden Un gen de interés y un gen marcador de seleccion que le confiere resistencia a un antibiotico o a un herbicida. El cultivo del explante se realiza en un medio de regeneracion con hormona vegetal, lo cual induce formacion de brotes. Se realiza el transplante del brote transgénico en tierra humeda (humedad relativa de menos de 80%) y con una intensidad lumínica entre 50-150 uE/m2/seg., estimulando la generacion de raíces y crecimiento del brote. Los métodos descritos no requieren germinacion, son rápidos y eficientes.
ARP080104043A 2007-03-12 2008-09-17 Metodo para producir una planta transgenica a partir del contacto de un explante con una construccion genetica AR068470A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/716,975 US20080229447A1 (en) 2007-03-12 2007-03-12 Transformation of immature soybean seeds through organogenesis
PCT/US2008/003308 WO2008112267A2 (en) 2007-03-12 2008-03-12 Transformation of immature soybean seeds through organogenesis

Publications (1)

Publication Number Publication Date
AR068470A1 true AR068470A1 (es) 2009-11-18

Family

ID=39759797

Family Applications (2)

Application Number Title Priority Date Filing Date
ARP080101016A AR065707A1 (es) 2007-03-12 2008-03-12 Transformacion de semillas de soya inmaduras a traves de organogenesis
ARP080104043A AR068470A1 (es) 2007-03-12 2008-09-17 Metodo para producir una planta transgenica a partir del contacto de un explante con una construccion genetica

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ARP080101016A AR065707A1 (es) 2007-03-12 2008-03-12 Transformacion de semillas de soya inmaduras a traves de organogenesis

Country Status (4)

Country Link
US (1) US20080229447A1 (es)
AR (2) AR065707A1 (es)
CL (1) CL2008000718A1 (es)
WO (2) WO2008112044A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2727575T3 (es) 2009-01-22 2019-10-17 Syngenta Participations Ag Polipéptidos de Hidroxifenilpiruvato Dioxigenasa mutantes, y Métodos de uso
US9347046B2 (en) 2009-01-22 2016-05-24 Syngenta Participations Ag Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
AR083029A1 (es) 2010-12-09 2013-01-23 Syngenta Participations Ag Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
AU2012284205B2 (en) 2011-07-15 2016-11-17 Syngenta Participations Ag Methods of increasing yield and stress tolerance in a plant
WO2013014585A1 (en) * 2011-07-22 2013-01-31 Basf Plant Science Company Gmbh Plant transformation method
US9861105B2 (en) 2011-07-28 2018-01-09 Syngenta Participations Ag Methods and compositions for controlling nematode pests
EP2856862A4 (en) * 2012-05-31 2015-12-30 Kaneka Corp PLANT TRANSFORMATION PROCESSES USING A PLANT FORCE-INFRINGING HORMON
CN103238395B (zh) * 2012-06-14 2014-11-26 西南大学 促种子发芽的方法
AU2013361491A1 (en) * 2012-12-19 2015-06-04 Dow Agrosciences Llc Improved soybean transformation for efficient and high-throughput transgenic event production
BR112016020670B1 (pt) 2014-03-07 2023-02-07 E.I. Du Pont De Nemours And Company Método de extração de um embrião, sistema para a extração de um embrião
US9924626B2 (en) 2014-05-06 2018-03-27 Dow Agrosciences Llc System for imaging and orienting seeds and method of use
MX2016014312A (es) 2014-05-06 2017-04-27 Dow Agrosciences Llc Sistema para preparacion de semillas y metodo de uso.
AR113761A1 (es) 2017-10-18 2020-06-10 Syngenta Participations Ag Control de plagas de hemípteros utilizando moléculas de arn
MX2021011836A (es) 2019-04-01 2021-10-22 Syngenta Crop Protection Ag Aumento de la transformabilidad de las plantas por transferencia de citotipo.
CN115605082A (zh) * 2019-11-26 2023-01-13 先正达农作物保护股份公司（Ch） 转化方法
WO2023192920A1 (en) * 2022-03-30 2023-10-05 Pairwise Plants Services, Inc. Methods for producing an organogenic callus and compositions, systems, and methods relating thereto

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310673A (en) * 1986-06-26 1994-05-10 Oji Paper Company, Ltd. Mass propagation through shoot primordia and regeneration of plants from protoplasts of shoot primordia
US5024944A (en) * 1986-08-04 1991-06-18 Lubrizol Genetics, Inc. Transformation, somatic embryogenesis and whole plant regeneration method for Glycine species
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
CA2036935A1 (en) * 1990-02-26 1991-08-27 Paul Christou Plant transformation process with early identification of germ line transformation events
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
CA2359868A1 (en) * 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
BR0113156A (pt) * 2000-08-11 2003-07-08 Syngenta Participations Ag Método para produzir um planta dicotiledÈnea transformada, célula de planta transformada, cultura de múltiplos brotos, planta transformada, semente produzida pela planta transformada, método para produzir uma planta compreendendo um genoma de plastìdeo transformado, genoma de plastìdeo transformado, e, plastìdeo
US20030046733A1 (en) * 2001-09-06 2003-03-06 Dias Kalyani Mallika Transformation of soybeans
CA2490154A1 (en) * 2002-06-22 2003-12-31 Syngenta Participations Ag Method of transforming soybean
WO2004074442A2 (en) * 2003-02-14 2004-09-02 Monsanto Technology Llc Plant regulatory sequences for selective control of gene expression

Also Published As

Publication number Publication date
CL2008000718A1 (es) 2008-05-16
WO2008112267A3 (en) 2008-11-06
WO2008112044A1 (en) 2008-09-18
WO2008112267A2 (en) 2008-09-18
AR065707A1 (es) 2009-06-24
WO2008112044A8 (en) 2008-12-11
US20080229447A1 (en) 2008-09-18

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