AR015805A1 - Transformacion del sorgo mediante agrobacterium - Google Patents
Transformacion del sorgo mediante agrobacteriumInfo
- Publication number
- AR015805A1 AR015805A1 ARP980101976A ARP980101976A AR015805A1 AR 015805 A1 AR015805 A1 AR 015805A1 AR P980101976 A ARP980101976 A AR P980101976A AR P980101976 A ARP980101976 A AR P980101976A AR 015805 A1 AR015805 A1 AR 015805A1
- Authority
- AR
- Argentina
- Prior art keywords
- sorghum
- transformed
- agrobacterium
- transformation
- plants
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8205—Agrobacterium mediated transformation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se presentan métodos y composiciones para la transformacion eficaz del sorgo. El método implica la infeccion de Agrobacterium, en especial las quecomprenden un vector superbinario. De este modo, todo gen de interés puede ser introducido en la plantade sorgo. El gen transformado estará flanqueado almenos por un borde de ADN-T y estará presente en el sorgo transformado en una baja cantidad de copias. Asimismo, se proporciona plantas, tejidos, células ysorgo transformado. La invencion comprende las plantas de sorgo fértiles y regeneradas y las semillas transgénicas producidas a partir de ellas T1 y lasgeneraciones subsiguientes.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US4512197P | 1997-04-30 | 1997-04-30 | |
US09/056,418 US6369298B1 (en) | 1997-04-30 | 1998-04-07 | Agrobacterium mediated transformation of sorghum |
Publications (1)
Publication Number | Publication Date |
---|---|
AR015805A1 true AR015805A1 (es) | 2001-05-30 |
Family
ID=26722402
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP980101976A AR015805A1 (es) | 1997-04-30 | 1998-04-29 | Transformacion del sorgo mediante agrobacterium |
Country Status (5)
Country | Link |
---|---|
US (2) | US6369298B1 (es) |
AR (1) | AR015805A1 (es) |
AU (1) | AU743706B2 (es) |
CO (1) | CO5290304A1 (es) |
WO (1) | WO1998049332A1 (es) |
Families Citing this family (59)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2267014A1 (en) * | 1999-03-26 | 2000-09-26 | Brenda Rojas | Monocot transformation using acrobacterium |
US6875907B2 (en) | 2000-09-13 | 2005-04-05 | Pioneer Hi-Bred International, Inc. | Antimicrobial peptides and methods of use |
CA2479739C (en) | 2002-03-20 | 2013-02-19 | J.R. Simplot Company | Refined plant transformation |
US20040133938A1 (en) * | 2002-11-19 | 2004-07-08 | Yinghui Dan | Use of lipoic acid in plant culture media |
US20060253917A1 (en) | 2002-12-26 | 2006-11-09 | Bret Cooper | Cell proliferation-related polypeptides and uses therefor |
WO2005086813A2 (en) | 2004-03-08 | 2005-09-22 | Syngenta Participations Ag | Glutamine-rich maize seed protein and promoter |
MX2007007948A (es) | 2004-12-28 | 2007-09-11 | Pioneer Hi Bred Int | Calidad de grano mejorada a traves de la expresion alterada de las proteinas de semilla. |
US7611898B2 (en) | 2005-05-09 | 2009-11-03 | The Samuel Roberts Noble Foundation | Agrobacterium transformation of stolons |
BRPI0717904B1 (pt) | 2006-12-07 | 2018-05-08 | Kansas State University Research Foundation | "método para controlar as ervas daninhas, método para produzir uma linhagem vegetal de híbrido de sorgo e método para identificar linhagens vegetais de sorgo". |
EP2114125B1 (en) | 2007-01-12 | 2013-12-04 | Kansas State University Research Foundation | Acetyl-coa carboxylase herbicide resistant sorghum |
WO2009006297A2 (en) | 2007-06-29 | 2009-01-08 | Pioneer Hi-Bred International, Inc. | Methods for altering the genome of a monocot plant cell |
EP2036983A1 (de) * | 2007-09-12 | 2009-03-18 | Bayer CropScience AG | Pflanzen, die erhöhte Mengen an Glucosaminglycanen synthetisieren |
WO2009082208A2 (en) | 2007-12-21 | 2009-07-02 | Keygene N.V. | Trichome specific promoters |
US8143484B2 (en) * | 2008-01-23 | 2012-03-27 | Pioneer Hi-Bred International, Inc. | Method for regulating agrobacterium-mediated transformation |
WO2010071418A1 (en) | 2008-12-19 | 2010-06-24 | Keygene N.V. | Recql5 in homologous recombination |
US8404930B2 (en) * | 2009-01-26 | 2013-03-26 | Pioneer Hi-Bred International, Inc. | Methods for improving monocot transformation |
US8373025B2 (en) | 2009-02-09 | 2013-02-12 | Chromatin Germplasm, Llc | Herbicide resistant sorghum |
MX336182B (es) | 2009-06-08 | 2016-01-11 | Nunhems Bv | Plantas tolerantes a la sequia. |
CN102892888A (zh) | 2009-12-30 | 2013-01-23 | 先锋国际良种公司 | 在植物中导入并调节表达基因的方法和组合物 |
CN107974457A (zh) | 2010-05-28 | 2018-05-01 | 纽海姆有限公司 | 果实大小增大的植物 |
US9617551B2 (en) * | 2010-07-29 | 2017-04-11 | Dow Agrosciences Llc | Method of increasing plant transformation frequency using modified strains of Agrobacteria |
EP2927323A3 (en) | 2011-04-11 | 2015-12-09 | Targeted Growth, Inc. | Identification and the use of krp mutants in plants |
BR112013027635A2 (pt) | 2011-04-29 | 2019-09-24 | Keygene Nv | sequência de nucleotídeos, enzima, vetor, hospedeiro, planta ou planta transgênica ou parte da mesma, semente, métodos para fornecer uma planta (célula) com resistência a glifosato, para acentuar resistência a glifosato de uma planta (célula), e para gerar um produto de planta, produto de planta, usos de uma sequência de nucleotídeos e de uma mutação |
WO2012159891A1 (en) | 2011-05-20 | 2012-11-29 | Syngenta Participations Ag | Endosperm-specific plant promoters and uses therefor |
US9365862B1 (en) | 2011-05-26 | 2016-06-14 | Nutech Ventures | Herbicide resistant sorghum mutants |
EP2714901A1 (en) | 2011-05-31 | 2014-04-09 | Keygene N.V. | Pest resistant plants |
WO2013048254A1 (en) | 2011-09-30 | 2013-04-04 | Keygene N.V. | Heat tolerance microrna |
WO2013048255A2 (en) | 2011-09-30 | 2013-04-04 | Keygene N.V. | Heat tolerance microrna |
US20140123339A1 (en) | 2012-10-31 | 2014-05-01 | Pioneer Hi Bred International Inc | Transformed Plants Having Increased Beta-Carotene Levels, Increased Half-Life and Bioavailability and Methods of Producing Such |
DK3062606T3 (da) | 2013-10-29 | 2019-06-24 | Biotech Inst Llc | Forædling, fremstilling, forarbejdning og anvendelse af speciel cannabis |
JP2018027020A (ja) * | 2014-12-24 | 2018-02-22 | 日本たばこ産業株式会社 | アグロバクテリウム菌を用いた、ソルガム属植物への遺伝子導入方法およびソルガム属植物の形質転換植物の作成方法 |
JP6978152B2 (ja) | 2015-09-04 | 2021-12-08 | キージーン ナムローゼ フェンノートシャップ | 複相胞子生殖遺伝子 |
WO2018087301A1 (en) | 2016-11-10 | 2018-05-17 | Keygene N.V. | Methods for improving drought resistance in plants - kgdr06, kgdr26, kgdr25, kgdr42 and kgdr37 |
WO2018101824A1 (en) | 2016-11-30 | 2018-06-07 | Universiteit Van Amsterdam | Plants comprising pathogen effector constructs |
WO2018146323A1 (en) | 2017-02-13 | 2018-08-16 | Keygene N.V. | Method for altering ripening characteristics of fruit |
WO2018146322A1 (en) | 2017-02-13 | 2018-08-16 | Keygene N.V. | Method for altering ripening characteristics of fruit |
CN108504672B (zh) * | 2018-03-30 | 2022-09-27 | 南京农业大学 | 青枯菌n477胞外蛋白phd及其编码基因和应用 |
AU2019246415A1 (en) | 2018-03-30 | 2020-09-17 | Keygene N.V. | QTLs for powdery mildew resistance in melon |
EP3775226A1 (en) | 2018-04-05 | 2021-02-17 | Keygene N.V. | Improved shoot regeneration by overexpression of chk genes |
NL2020823B1 (en) | 2018-04-25 | 2019-11-05 | Univ Delft Tech | NAD+ dependent 7ß-hydroxysteroid dehydrogenase |
EP3787395A1 (en) | 2018-05-01 | 2021-03-10 | Keygene N.V. | Genes for hormone-free plant regeneration |
JP7396770B2 (ja) | 2018-07-12 | 2023-12-12 | キージーン ナムローゼ フェンノートシャップ | 植物細胞におけるゲノム編集のためのv型crispr/ヌクレアーゼシステム |
EP3874048A1 (en) | 2018-11-01 | 2021-09-08 | Keygene N.V. | Dual guide rna for crispr/cas genome editing in plants cells |
AU2020225760A1 (en) | 2019-02-21 | 2021-08-19 | Keygene N.V. | Genotyping of polyploids |
NL2022905B1 (en) | 2019-04-09 | 2020-10-20 | Univ Delft Tech | Yeast with engineered Molybdenum co-factor biosynthesis |
NL2023169B1 (en) | 2019-05-21 | 2020-12-01 | Univ Delft Tech | Biotin prototrophy |
CA3138988A1 (en) | 2019-05-29 | 2020-12-03 | Keygene N.V. | Gene for parthenogenesis |
EP4075951A1 (en) | 2019-12-19 | 2022-10-26 | Keygene N.V. | Germacrene a synthase mutants |
WO2022079087A1 (en) | 2020-10-13 | 2022-04-21 | Keygene N.V. | Modified promoter of a parthenogenesis gene |
EP4291649A1 (en) | 2021-02-11 | 2023-12-20 | Koninklijke Nederlandse Akademie van Wetenschappen | Curing disease by transcription regulatory gene editing |
WO2022219175A1 (en) | 2021-04-15 | 2022-10-20 | Keygene N.V. | Mobile endonucleases for heritable mutations |
JP2024514169A (ja) | 2021-04-15 | 2024-03-28 | キージーン ナムローゼ フェンノートシャップ | 共再生難再生性植物体 |
EP4186917A1 (en) | 2021-11-29 | 2023-05-31 | Keygene N.V. | Tobamovirus resistant plants |
WO2023111225A1 (en) | 2021-12-17 | 2023-06-22 | Keygene N.V. | Double decapitation of plants |
WO2024008763A2 (en) | 2022-07-05 | 2024-01-11 | Keygene N.V. | Orobanche resistant plants |
WO2024023067A1 (en) | 2022-07-25 | 2024-02-01 | Koninklijke Nederlandse Akademie Van Wetenschappen | Curing disease by transcription regulatory gene editing |
WO2024047210A1 (en) | 2022-09-01 | 2024-03-07 | Keygene N.V. | Hydrogel-carriers for induction of plant morphogenesis |
US20240093220A1 (en) | 2022-09-09 | 2024-03-21 | Friedrich Alexander Universität Erlangen-Nürnberg | Plant regulatory elements and uses thereof |
WO2024084025A1 (en) | 2022-10-21 | 2024-04-25 | Keygene N.V. | Rna transfection in plant cells with modified rna |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5773691A (en) * | 1992-03-19 | 1998-06-30 | E. I. Du Pont De Nemours And Company | Chimeric genes and methods for increasing the lysine and threonine content of the seeds of plants |
ATE398679T1 (de) | 1992-07-07 | 2008-07-15 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
AU4469793A (en) | 1992-08-19 | 1994-02-24 | Monsanto Company | Method for transforming monocotyledonous plants |
PT672752E (pt) | 1993-09-03 | 2004-10-29 | Japan Tobacco Inc | Processo de transformacao de uma monocotiledonea com a utilizacao de um escutelode um embriao imaturo |
PT687730E (pt) | 1993-12-08 | 2007-07-02 | Japan Tobacco Inc | Método de transformação de plantas e vector para esse fim |
US5631152A (en) | 1994-10-26 | 1997-05-20 | Monsanto Company | Rapid and efficient regeneration of transgenic plants |
-
1998
- 1998-04-07 US US09/056,418 patent/US6369298B1/en not_active Expired - Lifetime
- 1998-04-14 AU AU71342/98A patent/AU743706B2/en not_active Ceased
- 1998-04-14 WO PCT/US1998/007849 patent/WO1998049332A1/en active IP Right Grant
- 1998-04-29 AR ARP980101976A patent/AR015805A1/es unknown
- 1998-04-29 CO CO98023424A patent/CO5290304A1/es not_active Application Discontinuation
-
2002
- 2002-03-19 US US10/101,342 patent/US20020138879A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
AU7134298A (en) | 1998-11-24 |
CO5290304A1 (es) | 2003-06-27 |
AU743706B2 (en) | 2002-01-31 |
US20020138879A1 (en) | 2002-09-26 |
US6369298B1 (en) | 2002-04-09 |
WO1998049332A1 (en) | 1998-11-05 |
WO1998049332A8 (en) | 1999-04-01 |
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