YU48050B - Enzimatski postupak za utvrđivanje antibiotika sa beta-laktam jezgrom u biološkoj tečnosti - Google Patents

Enzimatski postupak za utvrđivanje antibiotika sa beta-laktam jezgrom u biološkoj tečnosti

Info

Publication number
YU48050B
YU48050B YU78391A YU78391A YU48050B YU 48050 B YU48050 B YU 48050B YU 78391 A YU78391 A YU 78391A YU 78391 A YU78391 A YU 78391A YU 48050 B YU48050 B YU 48050B
Authority
YU
Yugoslavia
Prior art keywords
infirmation
lactam
antibiotics
corn
beta
Prior art date
Application number
YU78391A
Other languages
English (en)
Other versions
YU78391A (sh
Inventor
J. Degelaen
J.-M. Frere
Original Assignee
Ucb-Bioproducts S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ucb-Bioproducts S.A. filed Critical Ucb-Bioproducts S.A.
Publication of YU78391A publication Critical patent/YU78391A/sh
Publication of YU48050B publication Critical patent/YU48050B/sh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06086—Dipeptides with the first amino acid being basic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/825—Actinomadura
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

ENZIMATSKI POSTUPAK ZA UTVRĐIVANJE ANTIBIOTIKA SA BETA-LAKTAM JEZGROM U BIOLOŠKOJ TEČNOSTI koji obuhvata sledeće stupnjeve: /1/ inkubaciju pomenute biološke tečnosti sa rastvorljivom D-alanil-D-alanin-karboksipeptidazom koju proizvodi mikroorganizam Actinomadura R39, pri čemu se ta inkubacija izvodi pod uslovima koji omogućavaju da antibiotik beta-lak-tam, eventualno prisutan u biološkoj tečnosti, reaguje sa enzimom da bi obrazovao ekvimolekularan kompleks enzim-an-tiobiotik, neaktivan i uglavnom ireverzibilan; /2/ inkubaciju dobijene smeše po završetku stupanja /1/ sa rastvorom supstrata pod uslovifma koji omogućuju hidrolizu pomenutog supstrata pomoću enzima za obrazovanje jedinjenja, čija je količina srazmerna preostaloj enzimatskoj aktivnosti; /3/ utvrđivanje količine nastalog jedinjenja u stupnju /2/; /4/ poredjenje vrednosti utvrdjene u stupnju /3/ sa elalonom radi dobijanja koncentracije antibiotika u biološkoj tečnosti, n aznačen time, što se inkubacija u stupnju /2/ vrši sa supstratom koji je neki tioestar opšte formule u kojoj R1 predstavlja benzoil, fenilacetil ili n alfa-acetil-L-lisil radikal R2 predstavlja glicil ili D-alanil radikal R3 je atom vodonika ili metil radikal, tako da se hidrolizom obrazuje 2-merkaptoalkanoična kiselina formule u kojoj R3 ima značenje dato napred, a u prisustvu neke aminokiseline koja ubrzava hidrolizu supstrata pomoću enzima, pri čemu se pomenuta aminokiselina uzima iz reda aminokiselina konfiguracije D i glicina. Prijava sadrži još 2 nezavisna i 12 zavisnih zahteva.
YU78391A 1990-04-30 1991-04-30 Enzimatski postupak za utvrđivanje antibiotika sa beta-laktam jezgrom u biološkoj tečnosti YU48050B (sh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB909009692A GB9009692D0 (en) 1990-04-30 1990-04-30 An enzymatic method of determining beta-lactam ring antibiotics

Publications (2)

Publication Number Publication Date
YU78391A YU78391A (sh) 1994-01-20
YU48050B true YU48050B (sh) 1996-10-18

Family

ID=10675235

Family Applications (1)

Application Number Title Priority Date Filing Date
YU78391A YU48050B (sh) 1990-04-30 1991-04-30 Enzimatski postupak za utvrđivanje antibiotika sa beta-laktam jezgrom u biološkoj tečnosti

Country Status (22)

Country Link
US (1) US5246830A (sh)
EP (1) EP0468946B1 (sh)
JP (1) JP2823382B2 (sh)
AT (1) ATE124998T1 (sh)
AU (1) AU636482B2 (sh)
BG (1) BG61082B2 (sh)
CA (1) CA2040180C (sh)
CZ (1) CZ280282B6 (sh)
DE (1) DE69111149T2 (sh)
DK (1) DK0468946T3 (sh)
ES (1) ES2075414T3 (sh)
FI (1) FI97151C (sh)
GB (1) GB9009692D0 (sh)
GR (1) GR3017235T3 (sh)
HU (1) HU214926B (sh)
IE (1) IE69037B1 (sh)
NO (1) NO302664B1 (sh)
NZ (1) NZ237950A (sh)
PL (1) PL169360B1 (sh)
PT (1) PT97511B (sh)
SK (1) SK279176B6 (sh)
YU (1) YU48050B (sh)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001519533A (ja) * 1997-10-07 2001-10-23 ユセベ バイオプロダクツ，ソシエテ アノニム 液状乳製品中の分析物を測定するためのアッセイ装置
US6143513A (en) * 1999-06-23 2000-11-07 Biacore Ab Method and kit for detecting betalactam-containing compounds
PL385415A1 (pl) * 2008-06-11 2009-12-21 Marek Ciesielski Sposób wykrywania bakteriooporności, zestaw diagnostyczny oraz zastosowanie oznaczania obecności leku i/lub produktów jego rozpadu
EP2766735B1 (en) * 2011-10-14 2017-02-01 Université de Liège Method for measuring beta-lactam antibiotics
CN111830015B (zh) * 2019-04-18 2022-12-09 中国农业科学院农产品加工研究所 一种定量检测抗生素的方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2824863A (en) * 1952-11-14 1958-02-25 Ciba Pharm Prod Inc Acylmercapto compounds
US4166825A (en) * 1978-08-17 1979-09-04 Abbott Laboratories Chromogenic substrates
CA1185881A (en) * 1982-02-01 1985-04-23 Jacques Degelaen ENZYMATIC PROCESS FOR THE DETERMINATION OF .beta.-LACTAM ANTIBIOTICS
US4806478A (en) * 1984-10-17 1989-02-21 Minnesota Mining And Manufacturing Company Dry enzyme formulations containing D-amino acid oxidase
US4686182A (en) * 1984-10-17 1987-08-11 Minnesota Mining And Manufacturing Company Method and kit for detecting antibiotics in a liquid sample

Also Published As

Publication number Publication date
PT97511B (pt) 1998-08-31
ATE124998T1 (de) 1995-07-15
PL290061A1 (en) 1991-12-02
HU911441D0 (en) 1991-11-28
DK0468946T3 (da) 1995-10-09
IE911433A1 (en) 1991-11-06
EP0468946B1 (fr) 1995-07-12
CA2040180C (en) 2001-06-19
SK279176B6 (sk) 1998-07-08
NO911692D0 (no) 1991-04-29
JPH0686695A (ja) 1994-03-29
PL169360B1 (pl) 1996-07-31
CS9101246A2 (en) 1991-11-12
GB9009692D0 (en) 1990-06-20
CA2040180A1 (en) 1991-10-31
JP2823382B2 (ja) 1998-11-11
PT97511A (pt) 1992-01-31
FI97151C (fi) 1996-10-25
NO911692L (no) 1991-10-31
CZ280282B6 (cs) 1995-12-13
AU636482B2 (en) 1993-04-29
YU78391A (sh) 1994-01-20
DE69111149T2 (de) 1995-12-07
HUT58825A (en) 1992-03-30
HU214926B (hu) 1998-07-28
AU7622691A (en) 1991-11-07
IE69037B1 (en) 1996-08-07
NZ237950A (en) 1992-02-25
DE69111149D1 (de) 1995-08-17
GR3017235T3 (en) 1995-11-30
FI97151B (fi) 1996-07-15
FI912054A (fi) 1991-10-31
NO302664B1 (no) 1998-04-06
ES2075414T3 (es) 1995-10-01
BG61082B2 (bg) 1996-10-31
US5246830A (en) 1993-09-21
FI912054A0 (fi) 1991-04-29
EP0468946A1 (fr) 1992-01-29

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