WO2023035437A1 - 一种微藻培养基及降解藻毒素的方法 - Google Patents

一种微藻培养基及降解藻毒素的方法 Download PDF

Info

Publication number
WO2023035437A1
WO2023035437A1 PCT/CN2021/136046 CN2021136046W WO2023035437A1 WO 2023035437 A1 WO2023035437 A1 WO 2023035437A1 CN 2021136046 W CN2021136046 W CN 2021136046W WO 2023035437 A1 WO2023035437 A1 WO 2023035437A1
Authority
WO
WIPO (PCT)
Prior art keywords
culture medium
microalgae
sodium
microalgae culture
water
Prior art date
Application number
PCT/CN2021/136046
Other languages
English (en)
French (fr)
Inventor
黄成谭
潘军
叶蕾
黄敏
Original Assignee
海南绿藻世界生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 海南绿藻世界生物科技有限公司 filed Critical 海南绿藻世界生物科技有限公司
Publication of WO2023035437A1 publication Critical patent/WO2023035437A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Definitions

  • the invention relates to the technical field of microalgae cultivation, in particular to a microalgae culture medium and a method for degrading algae toxins.
  • Microalgae are one of the main producers in aquaculture ponds. In the aquaculture industry, there is a saying that water should be raised before raising fish. The color of the aquaculture water body before putting seedlings desired by research and cultivation technicians is light green, that is, the water body formed by microalgae as the dominant species. Microalgae are direct users of harmful substances in aquaculture water. As we all know, with the passage of breeding time, more feces, residual bait, and animal carcasses accumulate in the water, which will eventually directly lead to the increase of ammonia nitrogen and nitrite content, the increase of various stress responses of farmed animals, and the large-scale outbreak of diseases. Possibility increases.
  • beneficial algae is a difficult point in the industry at present in the process of cultivating outside ponds.
  • harmful algae such as cyanobacteria are prone to outbreaks and are more vigorous, and beneficial algae such as chlorella are not easy to survive.
  • Alkaloid neurotoxins are neuroexcitatory blockers that cause paralysis of the respiratory muscles of aquatic animals and lead to death. Cytotoxins attack various specific cells of animals, produce poison and cause damage.
  • the pathological changes after aquatic animal poisoning mainly include hepatomegaly, congestion, necrosis, intestinal bleeding, increased mucus on the body surface, partial or complete gill silk ulceration, etc.
  • the clinical manifestations include food refusal, wandering, paralysis, and death.
  • Cyanotoxins are toxic secondary metabolites released after bloom cyanobacteria cells rupture, and there are many types in fresh water. According to the toxic effect of toxins on organisms and the chemical composition and structure of toxins, cyanotoxins are mainly divided into two types: hepatotoxins that act on the liver and neurotoxins that act on the nervous system. Toxins, such as lipopolysaccharide, skin toxins, etc., mainly including microcystin, nodularin and cylindromycin
  • algal toxins can accumulate in fish gonads and cause various pathological damages. They can also lead to reduced fish spawning, lower fertilization rates, and lower survival rates of juvenile fish. And growth retardation, and has a parental transmission effect; in addition, algal toxins can also interfere with the secretion level of fish sex hormones and the expression of genes related to sex hormone synthesis.
  • Algal toxins will quickly bind to protein phosphatases in fish cells, inhibit protein phosphorylation, interfere with cell signal transduction, and cause cells to produce excessive reactive oxygen species, resulting in lipid peroxidation and DNA damage.
  • Algal toxins not only easily cause fish diseases, cause fish and shrimps to refuse to eat or even die, and affect the quality of aquatic products and breeding benefits. More importantly, they can enter the human body through the food chain, posing a serious threat to human health. Therefore, it is more and more urgent to analyze and study the poisoning mechanism, route, diagnosis method and detoxification method of algae toxins in pond culture to reduce or eliminate the harm to aquatic animals.
  • the invention provides a microalgae culture medium and a method for degrading algae toxins.
  • the medium can effectively promote the rapid growth and reproduction of beneficial microalgae, ensure the large-scale reproduction of beneficial microorganisms, accelerate the decomposition and transformation of organic matter in the aquaculture process, resist the erosion of harmful organisms, reduce algae toxins in water, and help improve aquaculture. environment and promote the growth of aquaculture products.
  • the invention provides a microalgae culture medium, which is composed of potassium persulfate, sodium dehydroacetate, sodium citrate, fruit acid, glacial acetic acid, sodium thiosulfate, lactis streptococcus, sodium lauroyl glutamate and Tween .
  • every 1L of microalgal culture medium consists of the following components and the balance of water:
  • the Tween is at least one of Tween 80 and Tween 60.
  • every 1L of microalgae culture medium is made up of the following components and the water of balance:
  • the invention also provides the application of the microalgae culture medium in promoting the growth of microalgae and degrading algae toxins.
  • microalgae are beneficial algae, including Chlorella, Oocystella, Nannochloropsis, Chaetoceros, and Thalassiosira.
  • the present invention also provides a preparation method for microalgae culture medium, comprising the steps of:
  • feed liquid 1 Cool the feed liquid 1 to normal temperature, add fruit acid, glacial acetic acid, and sodium thiosulfate in sequence, stir and mix evenly, and obtain feed liquid 2;
  • Streptococcus nisin was slowly added into the feed liquid 2 while stirring, and after being completely dissolved, sodium lauroyl glutamate and Tween were added in sequence, and water was added to make up the volume.
  • the ratio of the amount of water used in step 1) to that in step 3) is 1:1.
  • the invention also provides a method for degrading algal toxins, which comprises spraying the microalgae culture medium of the invention into an aquaculture pond.
  • the spraying amount of the culture medium is: 100-200ml of the culture medium is sprayed per mu of algae liquid culture pond, and the specific spraying amount can be 100ml or 200ml.
  • the invention relates to a microalgae culture medium and a method for degrading algae toxins.
  • the medium consists of potassium persulfate, sodium dehydroacetate, sodium citrate, fruit acid, glacial acetic acid, sodium thiosulfate, lactis streptococcus, sodium lauroyl glutamate and Tween.
  • the medium can effectively promote the rapid growth and reproduction of beneficial microalgae, ensure the large-scale reproduction of beneficial microorganisms, accelerate the decomposition and transformation of organic matter in the aquaculture process, resist the erosion of harmful organisms, reduce algae toxins in water, and help improve aquaculture. environment and promote the growth of aquaculture products.
  • the invention provides a microalgae culture medium and a method for degrading algal toxins.
  • Those skilled in the art can refer to the content of this article to appropriately improve the process parameters to achieve.
  • all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention.
  • the method and application of the present invention have been described through preferred embodiments, and relevant personnel can obviously make changes or appropriate changes and combinations to the method and application herein without departing from the content, spirit and scope of the present invention to realize and apply the present invention Invent technology.
  • test materials used in the present invention are all common commercially available products, which can be purchased in the market.
  • composition is shown in Table 1.
  • step 2 Place the material liquid in step 1 to cool to room temperature, then add three materials D, E, and F in sequence, and stir to mix evenly.
  • step 3 In the material liquid of step 2, slowly add material H while stirring. After it is completely dissolved, add material I and mix evenly.
  • Embodiment 4 cultivates microalgae, the method for degrading algae toxin
  • the culture medium of the present invention is not used, it needs to be stored at 0-4°C.
  • Experimental group use embodiment 1 of the present invention to carry out relevant breeding and detoxification operations, and use it after rainy days, and the amount of spraying is 200ml/mu of breeding pond.
  • Control group 1 using a commercially available detoxification product (trade name: Lvshuiwei) to carry out relevant breeding and detoxification operations, and use it after rainy days, the amount of spraying is 200ml/mu of breeding pond.
  • a commercially available detoxification product (trade name: Lvshuiwei) to carry out relevant breeding and detoxification operations, and use it after rainy days, the amount of spraying is 200ml/mu of breeding pond.
  • the algal toxin concentration in the culture pond was monitored every 2 hours.
  • Control group 1 Control group 2 0h 15mg/L 13mg/L 15mg/L 2 hours 4mg/L 11mg/L 23mg/L 4 hours 1mg/L 10mg/L 40mg/L 6 hours 0mg/L 8mg/L 80mg/L 8 hours 0mg/L 11mg/L 150mg/L 10h 0mg/L 24mg/L 230mg/L
  • control group 2 carry out the degradation algae toxin in the outer pond by the substratum described in the patent of the present invention, after the cloudy and rainy weather, the algae toxin in the culture pond begins to break out (control group 2), after using the substratum described in the present invention, the algae toxin Rapidly degrades and is completely removed within 6 hours.
  • control group 1 also had a certain degradation effect, the effect was not significant, and after maintaining for 6 hours, the algae toxin concentration rebounded, and the degradation effect failed. It can be seen that this patent has significant effect.
  • Three culture ponds were used to compare the water quality stability of the cultivation process with the culture medium of Example 1 of the present invention and the common detoxification in the market, and to monitor the changes of algae toxins in the cultivation process, as follows:
  • Example 1 of the present invention was used to maintain the stability of algae toxins in the relevant breeding process, and the specific use method was the same as that described in this patent.
  • Control group 1 using a commercially available detoxification product (trade name: Lvshuiwei) to carry out the operation of maintaining the stability of the relevant cultured algae toxins, with a daily spraying amount of 100ml/mu,
  • the algal toxin concentration in the culture pond was monitored every 2 days.
  • the culture medium of the present invention is used to maintain the stability of algal toxins during the breeding process, and the algal toxins are continuously maintained at 0, while the control group has increased to varying degrees. It can be seen that the effect of the medium of the present invention on decomposing algal toxins is remarkable.

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cultivation Of Seaweed (AREA)

Abstract

提供了一种微藻培养基及降解藻毒素的方法，该微藻培养基由过硫酸氢钾、脱氢乙酸钠、柠檬酸钠、果酸、冰乙酸、硫代硫酸钠、乳酸链球菌、月桂酰基谷氨酸钠和吐温组成。该培养基能够有效地促进有益微藻快速生长繁殖，保证有益微生物的大量繁殖，加快水产养殖过程有机物的分解转化，抵抗敌害生物的侵蚀，降低了水体中的藻毒素，有利于改善水产养殖环境、促进水产养殖产品的生长。

Description

一种微藻培养基及降解藻毒素的方法
本申请要求于2021年9月10日提交中国专利局、申请号为202111061438.7、发明名称为“一种微藻培养基及降解藻毒素的方法”的中国专利申请的优先权，其全部内容通过引用结合在本申请中。
技术领域
本发明涉及微藻培养技术领域，尤其涉及一种微藻培养基及降解藻毒素的方法。
背景技术
微藻是水产养殖塘塘主要的生产者之一。在水产养殖业内，有养鱼先养水的说法。研究和养殖技术人员所希望的放苗前的养殖水体颜色为浅绿色，即是微藻为优势种形成的水体。微藻是养殖水体中有害物质的直接利用者。众所周知，随着养殖时间的推移，水中的粪便、残饵、动物尸体累积较多，最终直接导致氨氮、亚硝酸盐含量聚升，养殖动物的各类应激反应增多增强，病害大规模爆发的可能性增大。但是，采取定向培藻的养殖方式，培养有益微藻的繁殖可促进有益微生物的大量生长，加快塘塘有机物的分解转化。藻通过自身光合作用，降低水体中氨氮、亚硝酸盐等有害物质含量，增加溶解氧含量，形成养殖水体中物质良性循环的环境。同样，微藻具有的强大修复水体的功能也使其可以应用在湖泊水体的污染治理上。因此，采用微藻来开发培水产品也有着非常好的市场前景。
但是，在外塘养殖过程中，如何定向培养有益藻是目前行业的难点，尤其是蓝藻等有害藻容易爆发，更具活力等优势下，有益的小球藻等藻类不易生存。
在阴雨天气，外塘藻类容易腐败变质，产生有毒的藻毒素，从而对鱼苗造成肝细胞损伤，小剂量慢性刺激可引发肝癌，破坏肝细胞结构。生物碱类神经毒素是神经兴奋阻断剂，导致水生动物呼吸肌麻痹而死亡。 细胞毒素则侵害动物的各种特定细胞，产生毒害，引起损伤。水生动物中毒后的病理变化主要有肝脏肿大、充血、坏死，肠道出血，体表黏液增多，鳃丝部分或全部溃烂等，临床表现有拒食、漫游、麻痹、死亡等。
蓝藻毒素是水华蓝藻细胞破裂后释放出来的有毒次级代谢产物，在淡水中的种类很多。根据毒素对生物的毒性作用及毒素的化学组成和结构，蓝藻毒素主要分为作用于肝脏的肝毒素和作用于神经系统的神经毒素2种类型，其它还有细胞毒性、皮肤毒性和刺激毒性藻毒素，如脂多糖、皮肤毒素等，主要包括微囊藻毒素(microcystin)、节球藻毒素(nodularin)和柱孢藻毒素
蓝藻水华及其毒素污染已成为饮用水安全，的主要危害之一，由此带来的水生态健康风险也是
当前研究的热点
蓝藻大量爆发会产生天然毒素，给水生生物造成严重危害，藻毒素可在鱼类性腺中累积并导致各种病理损伤，还可导致鱼类产卵量减少、受精率下降、幼鱼成活率降低并发育迟缓，且具有亲本传递效应；此外，藻毒素还会干扰鱼类性激素的分泌水平与性激素合成相关基因的表达。
藻毒素会迅速与鱼体细胞内的蛋白磷酸酶结合，抑制蛋白磷酸化，干扰细胞信号传导，还能导致细胞产生过量的活性氧簇，导致脂质过氧化，引起DNA损伤。
随着水产养殖业的发展，养殖品种的密度和产量在不断提高。伴随着颗粒饲料的大量使用，养殖水体的富营养化问题也日益加剧。近年来，水生动物在养殖过程中受到各种致病因素的影响，进而引发各种疾病，大规模死亡事件不断增加，除传统的氨氮、亚硝酸盐、硫化氢、重金属盐类、化学鱼药等之外，由藻类毒素引起的鱼虾病害和损失呈上升趋势。由于由藻类毒素引起的鱼虾中毒方面的研究不充分，同时当前水质检测项目也存在一定的局限性，因此对一些藻类毒素引起的中毒也很难做出准确的判断。目前，市场上的解毒产品越来越多，养殖户在面对五花八门的解毒产品时难以做出正确的选择而往往不知所措，只能频繁更换解毒产品，不仅造成浪费，还耽误病情造成更大损失。藻毒素不仅容易引 起鱼病，导致鱼虾拒食甚至死亡，影响水产品品质和养殖效益，更重要的是其可通过食物链进入人体，对人体健康造成严重威胁。因此对池塘养殖中藻类毒素的中毒机理、途径、诊断方法及解毒方式进行分析和研究以减轻或解除对水生动物的危害也越来越来迫切。
发明内容
有鉴于此，本发明提供了一种微藻培养基及降解藻毒素的方法。该培养基能够有效地促进有益微藻快速生长繁殖，保证有益微生物的大量繁殖，加快水产养殖过程有机物的分解转化，抵抗敌害生物的侵蚀，降低了水体中的藻毒素，有利于改善水产养殖环境、促进水产养殖产品的生长。
为了实现上述发明目的，本发明提供以下技术方案：
本发明提供一种微藻培养基，由过硫酸氢钾、脱氢乙酸钠、柠檬酸钠、果酸、冰乙酸、硫代硫酸钠、乳酸链球菌、月桂酰基谷氨酸钠和吐温组成。
一些实施方案中，每1L微藻培养基由如下组分和余量的水组成：
Figure PCTCN2021136046-appb-000001
本发明中，所述吐温为吐温80、吐温60中的至少一种。
一些具体实施例中，每1L微藻培养基由如下组分和余量的水组成：
Figure PCTCN2021136046-appb-000002
Figure PCTCN2021136046-appb-000003
或，
Figure PCTCN2021136046-appb-000004
或，
Figure PCTCN2021136046-appb-000005
本发明还提供了所述的微藻培养基在促进微藻生长、降解藻类毒素中的应用。
其中，所述微藻为有益藻类，包括小球藻、卵囊藻、微拟球藻、角毛藻、海链藻。
本发明还提供微藻培养基的制备方法，包括如下步骤：
1)在水中依次加入过硫酸氢钾、脱氢乙酸钠、柠檬酸钠，充分搅拌均匀至完全溶解，获得料液1；
2)将料液1冷却至常温，依次加入果酸、冰乙酸、硫代硫酸钠，搅拌混合均匀，获得料液2；
3)在所述料液2中边搅拌边缓慢加入乳酸链球菌，待完全溶解后，再依次加入月桂酰基谷氨酸钠、吐温，加水定容。
一些实施方案中，步骤1)中和步骤3)中所述水的用量之比为1:1。
本发明还提供一种降解藻毒素的方法，向水产养殖池中喷洒本发明的微藻培养基。
本发明中，所述培养基的喷洒量为：每亩藻液养殖池喷洒100～200ml所述培养基，具体喷洒量可为100ml或200ml。
本发明一种微藻培养基及降解藻毒素的方法。该培养基由过硫酸氢钾、脱氢乙酸钠、柠檬酸钠、果酸、冰乙酸、硫代硫酸钠、乳酸链球菌、月桂酰基谷氨酸钠和吐温组成。该培养基能够有效地促进有益微藻快速生长繁殖，保证有益微生物的大量繁殖，加快水产养殖过程有机物的分解转化，抵抗敌害生物的侵蚀，降低了水体中的藻毒素，有利于改善水产养殖环境、促进水产养殖产品的生长。
具体实施方式
本发明提供了一种微藻培养基及降解藻毒素的方法。本领域技术人员可以借鉴本文内容，适当改进工艺参数实现。特别需要指出的是，所有类似的替换和改动对本领域技术人员来说是显而易见的，它们都被视为包括在本发明。本发明的方法及应用已经通过较佳实施例进行了描述，相关人员明显能在不脱离本发明内容、精神和范围内对本文的方法和应用进行改动或适当变更与组合，来实现和应用本发明技术。
本发明采用的试材皆为普通市售品，皆可于市场购得。
下面结合实施例，进一步阐述本发明：
实施例1本发明培养基的制备
组成见表1。
表1培养基配方组成
序号 物料名称 重量
A 过硫酸氢钾 50g
B 脱氢乙酸钠 5g
C 柠檬酸钠 20g
D 果酸 35g
E 冰乙酸 35g
F 硫代硫酸钠 10g
G 乳酸链球菌 40g
H 月桂酰基谷氨酸钠 40g
I 吐温80 60g
配制方法：
1、将500ml纯化水煮沸，依次放入A、B、C三种物料，充分搅拌均匀至完全溶解。
2、将步骤1料液放置冷却至常温，再依次加入D、E、F三种物料，搅拌混合均匀。
3、在步骤2料液中，边搅拌边缓慢加入H物料、待完全溶解后，再加入I物料，混合均匀。
4、最后加入G物料，用纯化水定容至1L，即得本专利所述培养基。
实施例2本发明培养基的制备
配方组成见表2，配制方法同实施例1。
表2培养基配方组成
序号 物料名称 重量
A 过硫酸氢钾 80g
B 脱氢乙酸钠 10g
C 柠檬酸钠 25g
D 果酸 35g
E 冰乙酸 50g
F 硫代硫酸钠 15g
G 乳酸链球菌 40g
H 月桂酰基谷氨酸钠 50g
I 吐温80 80g
实施例3本发明培养基的制备
配方组成见表3，配制方法同实施例1。
表3培养基配方组成
序号 物料名称 重量
A 过硫酸氢钾 70g
B 脱氢乙酸钠 5g
C 柠檬酸钠 20g
D 果酸 40g
E 冰乙酸 35g
F 硫代硫酸钠 12g
G 乳酸链球菌 35g
H 月桂酰基谷氨酸钠 50g
I 吐温60 80g
实施例4培养微藻、降解藻毒素的方法
1、鱼、虾、贝类养殖初始，按照100ml/亩养殖池进行泼洒使用，连续泼洒5天。
2、养殖中后期，按照200ml/亩养殖池进行泼洒使用，每隔3天泼洒一次。
3、如遇阴雨天气，则在暴雨过后额外泼洒一次，使用量为100ml/亩养殖池。
4、本发明培养基，如未使用，则需要在0-4℃进行保存。
实施例5
将3口养殖塘，运用本发明实施例1培养基与市场上常见解毒素进行解毒比较，具体如下
实验组：运用本发明实施例1进行相关养殖解毒操作，在阴雨天后进行泼洒使用，泼洒量200ml/亩养殖池。
对照组1，运用市售解毒产品(商品名：绿水威)进行相关养殖解毒操作，在阴雨天后进行泼洒使用，泼洒量200ml/亩养殖池。
对照组2，不做任何解毒操作。
经解毒操作后，每隔2小时监测一次养殖池中藻毒素浓度。
结果如下：
表4
解毒时长 实验组 对照组1 对照组2
0h 15mg/L 13mg/L 15mg/L
2h 4mg/L 11mg/L 23mg/L
4h 1mg/L 10mg/L 40mg/L
6h 0mg/L 8mg/L 80mg/L
8h 0mg/L 11mg/L 150mg/L
10h 0mg/L 24mg/L 230mg/L
由以上数据可见:通过本发明专利所述培养基进行外塘降解藻毒素，在阴雨天气后，养殖池中藻毒素开始爆发(对照组2)，经使用本发明所述培养基后，藻毒素迅速降解，在6小时内已完全去除。而对照组1虽也有一定的降解作用但效果不显著，且在维持6小时后，藻毒素浓度反弹，降解作用失效。可见本专利具有显著作用。
实施例6
将3口养殖塘，运用本发明实施例1培养基与市场上常见解毒素进行养殖过程水质维稳比较，监控养殖过程藻毒素变化，具体如下：
实验组：采用本发明实施例1培养基进行相关养殖过程藻毒素维稳操作，具体同本专利所述使用方法。
对照组1，运用市售解毒产品(商品名：绿水威)进行相关养殖藻毒素维稳操作，每日泼洒量100ml/亩，
对照组2，不做任何解毒操作。
经解毒操作后，每隔2日监测一次养殖池中藻毒素浓度。
结果如下(单位：mg/L)：
表5
Figure PCTCN2021136046-appb-000006
Figure PCTCN2021136046-appb-000007
由以上数据可见，通过本发明培养基进行养殖过程藻毒素维稳操作，藻毒素持续维持在0，而对照组均有不同程度上升，可见本发明培养基分解藻毒素的效果显著。
以上所述仅是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本发明的保护范围。

Claims (10)

  1. 一种微藻培养基，其特征在于，由过硫酸氢钾、脱氢乙酸钠、柠檬酸钠、果酸、冰乙酸、硫代硫酸钠、乳酸链球菌、月桂酰基谷氨酸钠和吐温组成。
  2. 根据权利要求1所述的微藻培养基，其特征在于，每1L微藻培养基由如下组分和余量的水组成：
    Figure PCTCN2021136046-appb-100001
  3. 根据权利要求1或2所述的微藻培养基，其特征在于，所述吐温为吐温80、吐温60中的至少一种。
  4. 根据权利要求1所述的微藻培养基，其特征在于，每1L微藻培养基由如下组分和余量的水组成：
    Figure PCTCN2021136046-appb-100002
  5. 权利要求1～4任一项所述的微藻培养基在促进微藻生长、降解藻类毒素中的应用。
  6. 根据权利要求5所述的应用，其特征在于，所述微藻为有益藻类，包括小球藻、卵囊藻、微拟球藻、角毛藻、海链藻。
  7. 权利要求1～4任一项所述的微藻培养基的制备方法，其特征在于，包括如下步骤：
    1)在水中依次加入过硫酸氢钾、脱氢乙酸钠、柠檬酸钠，充分搅拌均匀至完全溶解，获得料液1；
    2)将料液1冷却至常温，依次加入果酸、冰乙酸、硫代硫酸钠，搅拌混合均匀，获得料液2；
    3)在所述料液2中边搅拌边缓慢加入乳酸链球菌，待完全溶解后，再依次加入月桂酰基谷氨酸钠、吐温，加水定容。
  8. 根据权利要求7所述的制备方法，其特征在于，步骤1)中和步骤3)中所述水的用量之比为1:1。
  9. 一种降解藻毒素的方法，其特征在于，向水产养殖池中喷洒将权利要求1～4任一项所述的培养基或权利要求7或8所述的制备方法制得的微藻培养基。
  10. 根据权利要求9所述的方法，其特征在于，所述培养基的喷洒量为：每亩藻液养殖池喷洒100～200ml所述培养基。
PCT/CN2021/136046 2021-09-10 2021-12-07 一种微藻培养基及降解藻毒素的方法 WO2023035437A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202111061438.7 2021-09-10
CN202111061438.7A CN113684170A (zh) 2021-09-10 2021-09-10 一种微藻培养基及降解藻毒素的方法

Publications (1)

Publication Number Publication Date
WO2023035437A1 true WO2023035437A1 (zh) 2023-03-16

Family

ID=78585947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2021/136046 WO2023035437A1 (zh) 2021-09-10 2021-12-07 一种微藻培养基及降解藻毒素的方法

Country Status (2)

Country Link
CN (1) CN113684170A (zh)
WO (1) WO2023035437A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113684170A (zh) * 2021-09-10 2021-11-23 海南绿藻世界生物科技有限公司 一种微藻培养基及降解藻毒素的方法
CN114621874B (zh) * 2021-12-28 2023-09-29 宁波浮田生物技术有限公司 一种微藻培养基及应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102391953A (zh) * 2011-10-26 2012-03-28 南昌大学 一种真菌介导的微藻收获的方法
CN103224889A (zh) * 2013-05-21 2013-07-31 青岛蔚蓝生物集团有限公司 一种微藻培养基及其应用
CN105886404A (zh) * 2016-06-14 2016-08-24 华东理工大学 一种可以抑制铜绿微囊藻生长的混养式微藻培养方法
CN106591131A (zh) * 2015-10-20 2017-04-26 青岛力天宏泰新能源科技有限公司 一种用于海洋微藻大规模培养的异养培养基
AU2020101711A4 (en) * 2020-08-07 2020-09-17 Tianjin Agricultural University A culture medium for culturing chlorella with high efficiency and practicability and application thereof
CN113308377A (zh) * 2021-06-16 2021-08-27 海南绿藻世界生物科技有限公司 一种微藻培养基及其培养方法和养殖水体的净化方法
CN113684170A (zh) * 2021-09-10 2021-11-23 海南绿藻世界生物科技有限公司 一种微藻培养基及降解藻毒素的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105217802B (zh) * 2015-10-29 2018-05-18 上海泰缘生物科技有限公司 一种水质净化剂
CN107117666A (zh) * 2017-05-11 2017-09-01 江苏苏沃尚新材料科技有限公司 一种水质净化剂
CN110786339A (zh) * 2019-11-15 2020-02-14 天津市创嘉生物技术有限公司 用于水产养殖的消毒剂及其制备方法
CN112010369A (zh) * 2020-08-26 2020-12-01 湖南科义生物科技有限公司 一种过硫复合盐水产养殖水质改良剂及其制备方法和其粉剂、片剂制备方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102391953A (zh) * 2011-10-26 2012-03-28 南昌大学 一种真菌介导的微藻收获的方法
CN103224889A (zh) * 2013-05-21 2013-07-31 青岛蔚蓝生物集团有限公司 一种微藻培养基及其应用
CN106591131A (zh) * 2015-10-20 2017-04-26 青岛力天宏泰新能源科技有限公司 一种用于海洋微藻大规模培养的异养培养基
CN105886404A (zh) * 2016-06-14 2016-08-24 华东理工大学 一种可以抑制铜绿微囊藻生长的混养式微藻培养方法
AU2020101711A4 (en) * 2020-08-07 2020-09-17 Tianjin Agricultural University A culture medium for culturing chlorella with high efficiency and practicability and application thereof
CN113308377A (zh) * 2021-06-16 2021-08-27 海南绿藻世界生物科技有限公司 一种微藻培养基及其培养方法和养殖水体的净化方法
CN113684170A (zh) * 2021-09-10 2021-11-23 海南绿藻世界生物科技有限公司 一种微藻培养基及降解藻毒素的方法

Also Published As

Publication number Publication date
CN113684170A (zh) 2021-11-23

Similar Documents

Publication Publication Date Title
CN101838614B (zh) 一种虾青素生产菌株及其诱变筛选方法与应用
CN106701622A (zh) 一种芽孢杆菌与小球藻混合剂及其制备方法和应用
WO2023035437A1 (zh) 一种微藻培养基及降解藻毒素的方法
TWI398219B (zh) 一種藻酸鈉用於提升對蝦屬生殖能力之用途
Daume The roles of bacteria and micro and macro algae in abalone aquaculture: a review
CN104585105B (zh) 凡纳滨对虾室外水体生态式育苗方法
CN113099989A (zh) 一种稻虾共作生态养殖方法
CN113308377A (zh) 一种微藻培养基及其培养方法和养殖水体的净化方法
CN113278557A (zh) 共生菌组合物及其制备方法和微藻的培养方法
CN108531408A (zh) 一株胶红酵母新菌株及微生态制剂
CN109548962A (zh) 一种包含植物乳杆菌的复合微生物菌剂及其应用
CN109430579A (zh) Em菌钙镁复合微生态制剂及其制备方法和使用方法
CN106083297A (zh) 一种水产养殖用培藻剂的制作方法
CN104488969B (zh) 一种抑制蓝藻的微生态制剂
CN106212392A (zh) 一种提高轮虫生长繁殖能力与强化其作为饵料营养的方法
CN105884426A (zh) 淡水虾养殖用培养基及其制备方法
CN104388327B (zh) 一种微生态制剂、饲料添加剂及预混料
CN109020667A (zh) 一种利用黑水虻养殖粪渣生产肥水料的方法及肥水料
CN112725186A (zh) 一种水产养殖用耐高温小球藻选育方法
CN104276669A (zh) 一种用于泥鳅养殖水的微生物净水剂及其制备方法
CN109006605B (zh) 一种南美白对虾淡水生态养殖方法
CN105815610A (zh) 一种水产养殖用培养基及其制备方法
CN110250047A (zh) 一种工厂化养殖四指马鲅的方法
CN111254080B (zh) 一株Nanochlorum sp.微藻及其作为水产饵料的应用
CN112450135A (zh) 一种北方池塘高效生态养虾的方法

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 21956606

Country of ref document: EP

Kind code of ref document: A1

NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 21956606

Country of ref document: EP

Kind code of ref document: A1