WO2022149634A1 - 저온 고압 효소 반응시킨 컬러 베이스 및 추출액을 함유하는 색조 화장료 조성물 - Google Patents
저온 고압 효소 반응시킨 컬러 베이스 및 추출액을 함유하는 색조 화장료 조성물 Download PDFInfo
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- WO2022149634A1 WO2022149634A1 PCT/KR2021/000252 KR2021000252W WO2022149634A1 WO 2022149634 A1 WO2022149634 A1 WO 2022149634A1 KR 2021000252 W KR2021000252 W KR 2021000252W WO 2022149634 A1 WO2022149634 A1 WO 2022149634A1
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- 229910052918 calcium silicate Inorganic materials 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000002737 cell proliferation kit Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
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- 230000001472 cytotoxic effect Effects 0.000 description 1
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- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
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- 230000002500 effect on skin Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
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- 210000003754 fetus Anatomy 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
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- 230000001788 irregular Effects 0.000 description 1
- 229940119170 jojoba wax Drugs 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229960003390 magnesium sulfate Drugs 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000037368 penetrate the skin Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
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- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
- A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/19—Cosmetics or similar toiletry preparations characterised by the composition containing inorganic ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9755—Gymnosperms [Coniferophyta]
- A61K8/9767—Pinaceae [Pine family], e.g. pine or cedar
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/42—Colour properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/60—Particulates further characterized by their structure or composition
- A61K2800/61—Surface treated
- A61K2800/62—Coated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Definitions
- the present invention is a powder of spongy bone fragments reacted with low-temperature and high-pressure enzyme; low-temperature and high-pressure enzyme-reacted color base; and a mixed extract of pine needles, elm root, evening primrose and arrowroot root subjected to low-temperature and high-pressure enzyme reaction; to a cosmetic composition containing a mixture as an active ingredient, wherein the cosmetic composition of the present invention has excellent long-term storage stability, There is an advantage in that it has excellent effects such as moisturizing power, covering power, color development power, and adhesion.
- makeup cosmetics include skin care functions such as skin nutrition supply, astringent effect, and moisturizing function, as well as functions to cover skin imperfections, arrange skin texture, and beautify skin by color. It can be said that the function of covering the blemishes and the function of giving color to the skin to beautify the skin are mostly performed by foundations among makeup cosmetics.
- the skin is a membrane that covers the outside of the body and occupies the widest part of the human body and functions to protect the body from the external environment.
- the skin is largely composed of three layers: the epidermis, the dermis, and the hypodermis. It acts organically to protect and regulate internal organs and other internal organs.
- the stratum corneum on the outermost surface of the epidermis is a flat, grayish-white tissue that has no vitality and is colorless and non-nucleated. This varies greatly from person to person depending on the skin type and skin condition, and is constantly replaced and eliminated. The closer the stratum corneum is to the surface of the skin, the less the adhesion between cells and the gaps between the cells. If the old dead skin cells are not removed, the skin tone becomes dull and the process of creating new cells is slowed, thereby promoting overall skin aging.
- the characteristic of aging skin is that the ability to exfoliate dead cells is reduced, the cell regeneration rotation cycle increases, the skin loses vitality and elasticity, and the elastic fibers and collagen fibers of the dermal layer of the skin are denatured, resulting in loss of elasticity.
- intrinsic aging which occurs with increasing age, regardless of external factors
- photoaging which is induced by long-term UV exposure. Skin damaged by intrinsic aging becomes dry and elasticity decreases, resulting in increased fine lines.
- photoaging increases the roughness and coarse wrinkles of the skin, and exhibits characteristics such as skin atrophy and loss of elasticity.
- the spongy bone fragment powder through the impregnation freeze-drying coating of the human umbilical cord blood cell culture solution of Republic of Korea Patent No. 10-2150798 (registration date: August 26, 2020) developed and patented by the present inventor A manufacturing method, and a cosmetic composition and cosmetic manufacturing method using the same are patented.
- the patented invention comprises the steps of: a) drying and pulverizing a sponge to prepare a sponge powder; b) mixing the sponge powder with distilled water; c) reacting the mixture with the enzyme in an enzyme reactor; d) washing the reacted mixture after centrifugation; e) heating the mixture of step d) in an electric furnace to obtain spongy bone fragments; and f) air-mixing and coating the skin active material while spraying the spongy bone fragments.
- the patented invention is a non-chemical method of high-temperature and high-pressure enzymatic decomposition method, which is purified by a high-temperature and high-pressure enzymatic decomposition method, the porous needle-type spongy bone powder is immersed in a solution in which a stem cell-derived material and an active skin ingredient are mixed, and then freeze-dried. Since it is a method that decomposes at high temperature in this method, there is a lot of waste of a solution in which stem cell-derived substances and skin active ingredients are mixed, and the manufacturing process is complicated.
- the present invention maximizes the efficiency of delivery into the skin of the human umbilical cord blood stem cell culture medium known to have anti-wrinkle and anti-aging effects in the prior art and to secure the safety of the compound dissolved or suspended in the mixture according to the physical properties of the mixture.
- the SPE Solid Phase Extraction
- the present invention is to disclose a method for producing a cosmetic composition using the spongy bone powder coated with an effective skin material as described above, and has the following objects.
- the present invention has been derived from the above needs, and in the present invention, a cosmetic composition with excellent storage stability and quality is provided by enzymatically reacting the spongy bone fragment powder, color base and mixed extract contained in the cosmetic composition under low temperature and high pressure conditions.
- Spongy bone fragment powder coated with human cord blood cell culture solution prepared by adding purified spongy bone fragment powder after bromelain enzyme treatment into the mixer, then adding human cord blood cell culture solution thereto and freeze-drying in a mixed state;
- It provides a cosmetic composition for moisturizing the skin containing a mixture of a mixture of pine needles, elm root, evening primrose and arrowroot root, which has been subjected to low-temperature and high-pressure enzyme reaction, as an active ingredient.
- spongy can be processed without chemical treatment, and human cord blood stem cell culture solution known to have anti-wrinkle and anti-aging effects
- human umbilical cord blood stem cell culture solution is delivered directly into the skin together with the needle-type spongy bone fragments by freeze-drying and coating on the needle-type spongy bone fragments purified by a non-chemically active method to maximize the conventional effect. It is possible to simultaneously achieve the function for beautiful and healthy skin regeneration by increasing self-immunity and normalizing the skin turnover cycle through stimulation.
- the color base (Color Base N-30) and the mixed extract (Extract N-30), as well as the spongy bone fragment powder, which are active ingredients in the cosmetic composition, make it easy to penetrate the skin by enzymatic reaction under low temperature and high pressure conditions, and aged at low temperature. It has the effect of increasing the safe active ingredient content through the process.
- Spongy bone fragment powder coated with human cord blood cell culture solution prepared by adding purified spongy bone fragment powder after bromelain enzyme treatment into the mixer, then adding human cord blood cell culture solution thereto and freeze-drying in a mixed state;
- It relates to a cosmetic composition containing, as an active ingredient, a mixture of pine needles, elm root, evening primrose and arrowroot root, which have undergone a low-temperature and high-pressure enzyme reaction.
- the spongy bone fragment powder which is the main component, is derived from the spicule of Sclerospongia, a river of sponges living in freshwater, so called sponge-spicule. call it This structure is in the form of a fine needle and is composed of calcium carbonate, silicate, and a jelly-shaped collagen matrix skeleton. Numerous pores are observed on the surface of the spicule, which feed and symbiotic green algae enter and exit.
- the spicule has a length of 0.01 mm to more than 1.0 mm, so its shape and size vary.
- stem cells in the present invention refer to undifferentiated cells that can differentiate into various types of tissues in embryos or adults.
- Stem cells are mainly harvested from embryos in the early division stage. Cells at this stage do not yet have the ability to form organs, so they can be cultured into a specifically selected cell line based on prior input.
- human umbilical cord blood cell culture medium (USC1994-FPBN) is a complex component containing 89 proteins obtained by culturing stem cells of 'umbilical cord blood' in the umbilical cord that connects the fetus and mother.
- a specific method for preparing the powder of spongy bone fragments purified after bromelain enzyme treatment is as follows.
- Pre-treating the sponge powder by drying and pulverizing the sponges collected from freshwater to a moisture content of less than 8% and separating them through a 70 mesh sieve;
- the pretreated sponge powder 50 g was diluted by adding 100 mL of PBS Buffer (Phosphate-Buffered Saline Buffer), and precipitation using DTT (0.12% w/v);
- PBS Buffer Phosphate-Buffered Saline Buffer
- the decomposed powder was purified by using a C18 cartridge to purify the organic material, and after precipitating a stationary phase in which the active ingredient remained, the precipitated powder was placed in an electric furnace, and the temperature was sequentially raised at 150°C for 20 minutes, at 350°C for 30 minutes, and at 750°C for 50 minutes. It can be prepared by the steps of
- the human umbilical cord blood cell culture medium was prepared by mixing USC 1994-FPBN powder mixed with mannitol, niacinamide, human cord blood cell conditioned media, and sodium hyaluronate in distilled water in a weight ratio of 1:2. It may be dissolved, but is not limited thereto.
- the air mixer may be an air mixer in which pressure reduction and cooling nitrogen input, and the temperature inside the air mixer is set to 20 °C, but is not limited thereto.
- the mixing is specifically performed by putting 100 g of spongy bone fragment powder into an air mixer and dispersing while adding cooling nitrogen to form an aerosol, and then visually checking the inside of the air mixer to ensure smooth dispersion.
- it is possible to air-mix after spraying the human umbilical cord blood cell culture solution at 3 ml/min, but is not limited thereto.
- the method for producing the spongy bone fragment powder coated with the human umbilical cord blood cell culture solution of the present invention described above is purified by a non-chemically active method in order to maximize the efficiency of delivery of the human umbilical cord blood stem cell culture medium, which is known to have anti-wrinkle and anti-aging effects, into the skin.
- the human umbilical cord blood stem cell culture solution is delivered directly into the skin along with the needle-type cavernous bone fragments to maximize the conventional effect, as well as increase autoimmunity through stimulation and normalize the skin turnover cycle, resulting in beautiful and beautiful skin.
- the function for healthy skin regeneration was achieved at the same time.
- Cosmetics containing the spongy bone flake powder coated with the human umbilical cord blood cell culture solution of the present invention as an active ingredient not only regenerates the skin of the human body, but also regenerates the skin according to the improvement of autoimmunity and wrinkle improvement after the inflammatory response caused by the physical stimulation of the needle-type spongy bone powder , whitening, skin elasticity improvement, skin aging prevention, skin moisture improvement, age spots removal, acne treatment and wound removal effects It was confirmed that the turnover cycle of epidermal cells is generally 28 days by promoting cell division, but the skin is regenerated while shortening it to about 72 hours.
- the active ingredient is directly delivered to the epidermis for about 24 to 36 hours while the lyophilized coated active ingredient of the acicular spongy bone fragment impregnated and coated with the human cortex blood-derived cell culture medium is absorbed in the needle-type spongy bone fragment. Furthermore, due to the increase in blood flow and the promotion of metabolism due to stimulation, the absorption and transfer of active ingredients is superior to that of general skin application. induce
- the color base is made of titanium dioxide, mica, zinc oxide, yellow iron oxide, red iron oxide, black iron oxide, triethoxycaprylylsilane, ultramarine, hydrogen dimethicone and dimethicone.
- the low-temperature and high-pressure enzymatic reaction of the color base is preferably performed by mixing 18 to 22% by weight of water with respect to the weight of the color base in the color base, followed by Protamex and Alcalase ( Alcalase) can be added to react for 20 to 28 hours at a low temperature of 30 to 45 ° C and a high pressure of 50 to 70 Mpa, and more preferably, after mixing 20 wt% of water with respect to the weight of the color base in the color base, Tamex (Protamex) and Alcalase (Alcalase) can be added to react for 24 hours at a low temperature of 30 ⁇ 45 °C and a high pressure of 60 Mpa.
- Protamex and Alcalase Alcalase
- the mixed extract is preferably pine needle extract, elm root extract, evening primrose extract and arrowroot extract in a weight ratio of 20-30:20-30:20-30:20-30.
- the mixed extract is aged at 1 ⁇ 8°C for 2 ⁇ 5 days, and then Protamex and Alcalase are added. It may be reacted for 28 hours, and more preferably, a mixed extract obtained by mixing pine needle extract, sugar elm root extract, evening primrose extract and arrowroot extract in a weight ratio of 25:25:25:25 is heated at 1 to 8 ° C. After aging for ⁇ 5 days, it may be reacted for 24 hours at a low temperature of 50°C and a high pressure of 60 Mpa by adding Protamex and Alcalase.
- the cosmetic composition is propylene glycol, ethylhexylmethoxycinnamate, emulsion stabilizer, caprylic/capric triglyceride, squalane, cetylethylhexanoate, dimethicone, Arbutin, ethylhexyl salicylate, phenyl trimethicone, diisostearyl malate, jojoba oil, magnesium sulfate, disteadimonium hectorite, beeswax, hydroxyacetophenone, panthenol, calcium stearate, fragrance , Aloe vera leaf juice, tocopheryl acetate, caprylyl glycol, allantoin, adenosine, mannitol, hexylcinnamal, butylphenylmethylpropional, niacinamide, citronellol, limonene, human cord blood cell
- the cosmetic may preferably be a color cosmetic, and the types include face powder, compact, powder foundation, two-way cake, make-up base, liquid foundation, cream foundation, eye shadow and It may be one color cosmetic selected from the group consisting of powder blush, but is not limited thereto.
- the sponge collected from freshwater was dried and pulverized so that the moisture content was less than 8%, and separated in a 70 mesh sieve to pre-treat the sponge powder.
- 100 mL of PBS Buffer Phosphate-Buffered Saline Buffer
- DTT 0.12% w/v
- the precipitated powder was centrifuged at 4°C, 15 minutes, and 16,200 rpm, and after centrifugation, heat denaturation was performed at 95°C for 3 minutes. After heat denaturation, 10 mL of bromelain enzyme was added and decomposed at 37° C. for 24 hours.
- organic matter is purified using a C18 cartridge and a stationary phase with the remaining active ingredients is precipitated.
- the precipitated powder is placed in an electric furnace and heated sequentially at 150°C for 20 minutes, at 350°C for 30 minutes, and at 750°C for 50 minutes. 100 g of the powder was prepared.
- Color Base N-30 includes titanium dioxide, mica, zinc oxide, yellow iron oxide, red iron oxide, black iron oxide, ultramarine, triethoxycaprylylsilane, hydrogen dimethicone, and dimethicone. 20 wt% purified water was mixed with Color Base N-30 prepared by mixing as shown in Table 1. Next, 3 g of Protamex and Alcalase were added in a weight ratio of 1:1. For the stability of the dye, the temperature of the enzyme reactor was lowered to 30-45° C., set to 60 Mpa, and then the enzyme reaction was performed for about 24 hours.
- the extract (Extract N-30) is a mixture of all of Daewang pine needle extract, elm root extract, evening primrose extract, and arrowroot extract, and the mixing ratio is as shown in Table 2 below.
- Color base (Color Base N-30) purified by low-temperature and high-pressure enzymatic reaction of Preparation Example 2;
- Cosmetics that have undergone a low-temperature aging step at 1 to 8°C for 72 hours after mixing them in the mixing ratio of Table 3 using the extract (Extract N-30) purified by the low-temperature and high-pressure enzyme reaction of Preparation Example 3.
- Color base (Color Base N-30) purified by low-temperature and high-pressure enzymatic reaction of Preparation Example 2;
- Sponge bone fragment powder refined by chemical method (a method of removing impurities by putting it in hydrochloric acid and drying it),
- Color base (Color Base N-30) prepared by the method of Preparation Example 2, but not subjected to low-temperature and high-pressure enzymatic reaction,
- HaCaT cells were cultured in DMEM (Dulbecco's Modified Eagle Medium) containing 10% Fetal bovine serum (FBS) and 1% penicillin-streptomycin (PS, Gibco) at 37° C., 5% CO 2 condition.
- DMEM Dulbecco's Modified Eagle Medium
- FBS Fetal bovine serum
- PS penicillin-streptomycin
- Each sample was diluted by concentration in the cultured HaCaT cells (human keratinocyte line) for 24 hours and treated with MTS assay (CellTiter Aqueous One Solution Cell proliferation assay kit 3-(4,5-dimethylthiazol-2-yl)) -5-(3-carboxymethoxyphenyl)-2-(4-sulfophynyl)-2H-tetrazolium, inner salt; MTS, Promega Co. Madison, WI, USA) method to measure cell viability at 490 nm with a microplate reader (Molecular Devices, Sunnyvale, CA, USA).
- MTS assay CellTiter Aqueous One Solution Cell proliferation assay kit 3-(4,5-dimethylthiazol-2-yl)) -5-(3-carboxymethoxyphenyl)-2-(4-sulfophynyl)-2H-tetrazolium, inner salt; MTS, Promega Co. Madison, WI, USA
- Hyaluronic acid content was compared with single extract, evening primrose single extract with low temperature and high pressure enzyme reaction, and arrowroot root single extract with low temperature and high pressure enzyme reaction.
- each sample was treated on the HaCaT cells for 24 hours, irradiated with ultraviolet light (UV Crosslinker, Ultra Lum) for 30 minutes at 20 mJ/cm 2 , the supernatant of the culture was collected, and a hyaluronic acid ELISA kit (R&D Systems, Inc., Minneapolis, MN, USA) was used to measure the content of hyaluronic acid (Hyaluronic Acid).
- UV Crosslinker, Ultra Lum ultraviolet light
- Hyaluronic Acid hyaluronic Acid
- Hyaluronic acid content of extract Extract (300 ⁇ g/mL) Hyaluronic acid (ng/ml) No treatment (no added extract mixture, no UVB treatment) 42.3 No addition of mixed extract + UVB treatment 12.4
- Preparation Example 3 (low temperature and high pressure enzyme reaction mixture extract) + UVB treatment 32.5 Mixed extract without low-temperature and high-pressure enzyme treatment + UVB treatment 29.4
- Low-temperature and high-pressure enzyme reaction Daewang pine needle extract 100% + UVB treatment 20.1
- Example 1 In order to examine the skin moisturizing effect with the foundations of Example 1 and Comparative Examples 1 and 2, the skin conductivity was measured, and as a result, Example 1 showed the highest.
- Example 1 As a result of measuring and evaluating an L* value indicating brightness (skin brightness) using a chromameter, Example 1 showed the highest.
- Example 1 and Comparative Examples were subjected to temperature 30 ⁇ 2° C./relative humidity 66 ⁇ 5% for 1 month, 3 months, and 6 months, and the separation phenomenon of the emulsified state and the dispersed state was observed and evaluated as a score. .
- Example 1 Comparative Example 1 Comparative Example 2 emulsified state 1 month after storage 5 5 5 3 months after storage 5 5 4 After storage for 6 months 5 4 4 separation phenomenon 1 month after storage 5 5 5 3 months after storage 5 5 4 After storage for 6 months 5 4 3
- Example 1 As a result, even if the foundation of Example 1 was stored for 6 months, the emulsification state was good, the separation phenomenon did not occur, and it was confirmed that the long-term storage stability was high.
- the score is the average value of 30 responses (however, the brighter the color, the closer to 10).
- Example 1 As a result, the cosmetics of Example 1 showed a high score of 9 or more in all items, confirming that the feeling of use was excellent.
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
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JP2021516641A JP7329588B2 (ja) | 2021-01-08 | 2021-01-08 | 低温高圧酵素を反応させたカラーベースおよび抽出液を含有する色調化粧料組成物 |
JP2023126906A JP7371298B2 (ja) | 2021-01-08 | 2023-08-03 | 低温高圧酵素を反応させたカラーベースおよび抽出液を含有する色調化粧料組成物 |
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KR20190047582A (ko) * | 2017-10-27 | 2019-05-08 | 주식회사 케미랜드 | 식물성 소재의 2단계 순차적 효소처리 추출물과 이를 포함하는 기능성 화장료 조성물 및 그 제조방법 |
KR102150798B1 (ko) * | 2019-06-12 | 2020-09-01 | 주식회사 다이오홀딩스 | 인체 제대혈 세포 배양액의 함침 동결 건조 코팅을 통한 해면 골편 파우더의 제조 방법, 및 이를 이용한 화장료 조성물 및 화장품 제조 방법 |
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KR20190047582A (ko) * | 2017-10-27 | 2019-05-08 | 주식회사 케미랜드 | 식물성 소재의 2단계 순차적 효소처리 추출물과 이를 포함하는 기능성 화장료 조성물 및 그 제조방법 |
KR102150798B1 (ko) * | 2019-06-12 | 2020-09-01 | 주식회사 다이오홀딩스 | 인체 제대혈 세포 배양액의 함침 동결 건조 코팅을 통한 해면 골편 파우더의 제조 방법, 및 이를 이용한 화장료 조성물 및 화장품 제조 방법 |
KR20200144904A (ko) * | 2019-06-19 | 2020-12-30 | (주)아모레퍼시픽 | 혼합 사용이 가능한 채도 조절용 화장료 조성물 |
KR102165564B1 (ko) * | 2019-07-22 | 2020-10-14 | 주식회사 오퍼스아시아 | 모공축소 효과가 우수한 화장료 조성물 |
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CN117487782A (zh) * | 2023-09-19 | 2024-02-02 | 广州旭朗生物科技有限公司 | 一种水解海绵及其提取和应用 |
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KR102242215B1 (ko) | 2021-04-20 |
JP2023502802A (ja) | 2023-01-26 |
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