WO2020042697A8 - 一种重组微生物、其制备方法及其在生产辅酶q10中的应用 - Google Patents

一种重组微生物、其制备方法及其在生产辅酶q10中的应用 Download PDF

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Publication number
WO2020042697A8
WO2020042697A8 PCT/CN2019/089437 CN2019089437W WO2020042697A8 WO 2020042697 A8 WO2020042697 A8 WO 2020042697A8 CN 2019089437 W CN2019089437 W CN 2019089437W WO 2020042697 A8 WO2020042697 A8 WO 2020042697A8
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Prior art keywords
recombinant microorganism
preparation
application
method therefor
coenzyme
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PCT/CN2019/089437
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English (en)
French (fr)
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WO2020042697A1 (zh
Inventor
于洪巍
袁慎峰
朱永强
潘梦垚
于凯
陈志荣
李永
邱贵生
刘晓庆
Original Assignee
浙江新和成股份有限公司
黑龙江新和成生物科技有限公司
上虞新和成生物化工有限公司
浙江大学
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Application filed by 浙江新和成股份有限公司, 黑龙江新和成生物科技有限公司, 上虞新和成生物化工有限公司, 浙江大学 filed Critical 浙江新和成股份有限公司
Priority to KR1020207032816A priority Critical patent/KR102473375B1/ko
Priority to US17/271,356 priority patent/US20210324391A1/en
Priority to JP2020544025A priority patent/JP7072809B2/ja
Priority to DE112019000467.0T priority patent/DE112019000467T5/de
Publication of WO2020042697A1 publication Critical patent/WO2020042697A1/zh
Publication of WO2020042697A8 publication Critical patent/WO2020042697A8/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/66—Preparation of oxygen-containing organic compounds containing the quinoid structure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

提供了一种重组微生物、其制备方法及其在生产辅酶Q10中的应用。具体地，提供一种外源导入编码全局调控蛋白irrE的基因来构建重组微生物的方法，该重组微生物适用于发酵法生产辅酶Q10，特别适用于生产氧化型辅酶Q10。
PCT/CN2019/089437 2018-08-28 2019-05-31 一种重组微生物、其制备方法及其在生产辅酶q10中的应用 WO2020042697A1 (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020207032816A KR102473375B1 (ko) 2018-08-28 2019-05-31 재조합 미생물, 그 제조방법 및 보효소 q10의 생산에 있어서 그의 사용
US17/271,356 US20210324391A1 (en) 2018-08-28 2019-05-31 Recombinant microorganism, preparation method therefor and application thereof in producing coenzyme q10
JP2020544025A JP7072809B2 (ja) 2018-08-28 2019-05-31 組換え微生物、その製造方法及び補酵素ｑ１０の生産における使用
DE112019000467.0T DE112019000467T5 (de) 2018-08-28 2019-05-31 Rekombinanter Mikroorganismus, Verfahren zu dessen Herstellung und seine Anwendung bei der Herstellung von Coenzym Q10

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201810984954.9A CN109055417B (zh) 2018-08-28 2018-08-28 一种重组微生物、其制备方法及其在生产辅酶q10中的应用
CN201810984954.9 2018-08-28

Publications (2)

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WO2020042697A1 WO2020042697A1 (zh) 2020-03-05
WO2020042697A8 true WO2020042697A8 (zh) 2020-09-10

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PCT/CN2019/089437 WO2020042697A1 (zh) 2018-08-28 2019-05-31 一种重组微生物、其制备方法及其在生产辅酶q10中的应用

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Country Link
US (1) US20210324391A1 (zh)
JP (1) JP7072809B2 (zh)
KR (1) KR102473375B1 (zh)
CN (1) CN109055417B (zh)
DE (1) DE112019000467T5 (zh)
WO (1) WO2020042697A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109055417B (zh) * 2018-08-28 2020-07-07 浙江新和成股份有限公司 一种重组微生物、其制备方法及其在生产辅酶q10中的应用
WO2020223923A1 (en) * 2019-05-08 2020-11-12 Inner Mongolia Kingdomway Pharmaceutical Co., Ltd. Methods for a controlled coenzyme q10 fermentation production process

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2428248A1 (en) 2000-11-21 2002-08-08 Irm Llc Solubility reporter gene constructs
TWI305547B (en) 2001-12-27 2009-01-21 Kaneka Corp Processes for producing coenzyme q10
CN101418300B (zh) * 2007-10-22 2010-12-08 中国农业科学院生物技术研究所 提高植物耐盐及抗旱性的基因及其应用
CN101671679B (zh) * 2009-04-30 2013-09-18 浙江大学 一种耐辐射球菌抗逆相关基因及其应用
CN103509729B (zh) 2012-06-15 2016-09-28 浙江新和成股份有限公司 一种生产辅酶q10工程菌的构建方法、工程菌及其应用
PL2700715T3 (pl) * 2012-08-20 2019-03-29 Evonik Degussa Gmbh Sposób fermentacyjnego wytwarzania L-aminokwasów z zastosowaniem ulepszonych szczepów z rodziny Enterobacteriaceae
CN104561154B (zh) 2014-12-30 2020-01-10 内蒙古金达威药业有限公司 一种辅酶q10发酵工艺及控制策略
CN104830873B (zh) * 2015-05-11 2017-06-30 中国农业科学院生物技术研究所 一种位点突变的嗜热异常球菌IrrE蛋白及其应用
CN105420417B (zh) 2015-10-26 2019-04-30 上虞新和成生物化工有限公司 基于在线氧消耗速率和电导率协同控制的辅酶q10发酵生产工艺
CN106520653B (zh) * 2016-12-02 2019-05-07 天津科技大学 高产电活性和环境胁迫耐受性的基因工程菌
CN107868795B (zh) * 2017-10-18 2021-02-02 华东理工大学 利用乙酸生产丙酮或异丙醇的代谢工程大肠杆菌菌株的构建方法及应用
CN107699535B (zh) * 2017-11-08 2021-07-06 光明乳业股份有限公司 一种诱导合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用
CN108048496B (zh) 2017-12-25 2020-11-10 浙江新和成股份有限公司 氧化型辅酶q10的发酵生产方法、及由其制备而得的高含量氧化型辅酶q10
CN109055417B (zh) * 2018-08-28 2020-07-07 浙江新和成股份有限公司 一种重组微生物、其制备方法及其在生产辅酶q10中的应用

Also Published As

Publication number Publication date
JP7072809B2 (ja) 2022-05-23
JP2021517806A (ja) 2021-07-29
WO2020042697A1 (zh) 2020-03-05
KR20210005632A (ko) 2021-01-14
US20210324391A1 (en) 2021-10-21
CN109055417A (zh) 2018-12-21
DE112019000467T5 (de) 2020-10-01
KR102473375B1 (ko) 2022-12-01
CN109055417B (zh) 2020-07-07

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