WO2020019929A1 - Compound chinese traditional medicine antibacterial composition and application thereof in toiletry products - Google Patents

Abstract

A compound Chinese traditional medicine antibacterial composition and an application thereof in preparing toiletry products; the composition comprises 1-10 parts of Melaleuca alternifolia leaf powder, 0.1-5 parts of Melaleuca alternifolia leaf oil, 1-10 parts of Scutellaria baicalensis extract, and 0.05-0.5 parts of Cymbopogon nardus oil. The composition has the effects of inhibiting pathogenic microorganisms, balancing skin surface flora, isolating and removing harmful substances on the skin, and enhancing the nourishment of the skin cells.

Description

Compound traditional Chinese medicine antibacterial composition and application thereof in washing products Technical field

The present invention relates to the technical field of toiletries, in particular to a compound traditional Chinese medicine antibacterial composition and its application in toiletries.

Background technique

With the improvement of people's consumption awareness, consumers are paying more and more attention to the safety of products when choosing washing and cleaning products, and pursuing natural skin care. Toiletries containing a variety of medicinal plant extracts can meet people's needs for natural, green, Health needs. However, most of the toiletries on the market are mainly cleansing, lacking real antibacterial effects, or adding prohibited ingredients, which are highly irritating and cause secondary damage to the skin.

Summary of the Invention

Based on this, it is necessary to provide a natural compound traditional Chinese medicine antibacterial composition capable of effectively antibacterial and its application in washing products.

A compound traditional Chinese medicine antibacterial composition, based on parts by weight, includes the following components:

In one embodiment, the compound traditional Chinese medicine antibacterial composition includes, by weight parts, the following components:

In one embodiment, the compound traditional Chinese medicine antibacterial composition includes, by weight parts, the following components:

In one embodiment, the Scutellaria baicalensis extract is prepared by the following method:

Weigh the root bark of Scutellaria baicalensis and boil it with water, filter out the solids and obtain.

In one of the embodiments, the method of boiling comprises adding 250ml to 350ml of water for every 15g of the root bark of Scutellaria baicalensis, and boiling for 30min to 60min until the remaining liquid is 80ml to 120ml.

In one embodiment, the Alternate Leaf Melaleuca Leaf Powder is prepared by the following method:

Fresh branches with a single branch diameter of not more than 0.5 cm and the leaves thereon are selected, and dried, crushed and sieved to obtain it.

In one embodiment, the screening uses a 60-180 mesh screen.

The invention also provides the application of the compound traditional Chinese medicine antibacterial composition of any of the above embodiments in the preparation of toiletries.

The present invention also provides a washing and protecting article, comprising a washing and protecting matrix, a solvent, and the compound traditional Chinese medicine antibacterial composition according to any one of the foregoing embodiments.

The invention also provides a method for preparing the toiletries, comprising the following steps:

The compound traditional Chinese medicine antibacterial composition is mixed with the solvent, and then added to the washing and protecting matrix.

In one embodiment, the solvent is propylene glycol and / or butanediol.

The raw materials of the compound traditional Chinese medicine antibacterial composition of the present invention are introduced as follows:

Alternate Leaf Melaleuca Leaf Oil, also known as Australian Tea Tree Essential Oil, is a pure natural plant extract and a medium-efficient fungicide. Alternate leaf millet leaf oil can antibacterially remove mites, condense pores, and has a strong percutaneous penetration promotion effect, which can help the absorption of other active ingredients in the component. The main chemical constituents of Alpinia latifolia oil include 4-terpineol and 1,8-eucalyptol. The antibacterial activity of essential oils increases with the concentration of 4-terpineol under certain conditions. Eucalyptus is related to the irritation of essential oils. The lower the concentration of 1,8-eucalyptin, the less the irritation.

Alternate leaf white lamellar leaf powder contains volatile oil, terpineol, olefins and other ingredients. In addition to the granular powder containing more than 1% of leaf oil, it can also play a massage role in the washing process, which can remove the dry and aging skin surface Cuticle, removes dirt and sebum blocking pores, regulates sebum metabolism and improves skin environment.

Scutellaria baicalensis extract contains baicalin, baicalein, flavonoids, amino acids, sugars, etc., and has antibacterial, anti-inflammatory, anti-allergic, anti-oxidant, scavenging free radicals, and conditioning skin. Scutellaria baicalensis extract can clear heat and detoxify, disperse stasis and hemostasis, diuretic and swelling; inhibit elastase secretion, eliminate free radicals, thereby increase skin metabolism, skin elasticity and anti-wrinkle, and play an anti-aging role; can promote brain amide secretion, and Improve sebum and make skin soft; can inhibit dermatitis, enhance skin resistance, have anti-inflammatory properties; can neutralize released histamine, and have the ability to prevent allergies.

Asian citronella oil contains citronellal, geraniol, and citronellol, which can be used to clean oily skin, play a role in sterilizing and anti-inflammatory, soothing muscles, relieving pain, and softening the skin. Asian citronella oil has a strong scent of green pepper, refreshing the mind and eliminating brain fatigue. At the same time, it can reconcile the odor of alternate leaf white laminaria leaf oil and make the components smell more harmonious and more comfortable.

Compared with the existing solutions, the present invention has the following beneficial effects:

In the above-mentioned compound Chinese medicine antibacterial composition, each component is matched according to a specific ratio, and a variety of functional ingredients are rich in harmony and synergy with each other to kill pathogenic bacteria such as E. coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa. The killing effect is obviously better than the effect of each component when used alone.

The above-mentioned compound traditional Chinese medicine antibacterial composition can play a role in inhibiting pathogenic microorganisms, balancing the surface flora of the skin, isolating and removing harmful substances outside the skin, and strengthening skin cell nutrition. The selected components are natural plant ingredients, green and environmentally friendly, non-toxic and side effects, mild and non-irritating.

The test results show that the skin cream containing the above-mentioned compound traditional Chinese medicine antibacterial composition has obvious therapeutic effect on acne, and the skin cream containing only a single medicinal ingredient has no curative effect on acne, or the cure rate is low, and the above-mentioned compound traditional Chinese medicine antibacterial The skin cream of the composition can significantly improve the cure rate of acne in a short time.

detailed description

In order to facilitate understanding of the present invention, the present invention will be described more fully with reference to specific embodiments. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough understanding of the present disclosure.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to limit the invention.

The alternate leaf millet leaf powder used in the following examples and comparative examples is prepared by a method including the following steps:

Select fresh branches with a single branch not larger than 0.5 cm in diameter and the leaves on them, wash them with water, dry them, cut the branches to about 2 cm per section, pulverize them with a grinder, and use 60 to Sieving 180 mesh screen 3 times, that's it.

The alternate leaf white lamellar oil used in the following examples and comparative examples is prepared by a method including the following steps:

Fresh branches and leaves of Melaleuca alternifolia were selected, heated and extracted by water distillation equipment, and condensed to separate crude oil and water to obtain crude oil. The refined equipment filtered out harmful substances and retained most of the active ingredients in the Australian tea tree.

The Scutellaria baicalensis extract used in the following examples and comparative examples is prepared by a method including the following steps:

Weigh the root bark of Scutellaria baicalensis and add 250ml to 350ml of water for every 15g of the root bark of Scutellaria baicalensis. Boil for 30min to 60min until the remaining liquid is 80ml to 120ml. Filter through a 50-mesh filter to obtain the Scutellaria baicalensis extract. Seal it in a refrigerator at 4 ° C. Save.

The citronella oil used in the following examples and comparative examples is the same commercially available product.

Example 1

Weigh 1g of S. alternifolia leaf powder, 5g of S. alternifolia leaf oil, 10g of Scutellaria baicalensis extract and 0.05g of citronella oil, and dissolve it in a propylene glycol solvent to obtain a 10% by weight compound Chinese medicine antibacterial composition mixture.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Example 2

Weigh 10g of Alternate Leaf Melaleuca Leaf Powder, 0.1g of Alternate Leaf Melaleuca Leaf Oil, 1g of Scutellaria Baicalensis Extract and 0.5g of Citronella Oil, and dissolve it in a propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution with a concentration of 10% by weight. .

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Example 3

Weigh 5g of S. alternifolia leaf powder, 3g of S. alternifolia leaf oil, 8g of Scutellaria baicalensis extract and 0.05g of citronella oil, and dissolve it in a propylene glycol solvent to obtain a 10% by weight compound traditional Chinese medicine antibacterial composition mixture.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Example 4

Weigh 8g of Alternate Leaf Melaleuca Leaf Powder, 0.5g of Alternate Leaf Melaleuca Leaf Oil, 3g of Scutellaria Baicalensis Extract and 0.3g of Citronella Oil, and dissolve it in a propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution with a concentration of 10% by weight. .

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Example 5

Weigh 5g of Alternate Leaf Melaleuca Leaf Powder, 2.5g of Alternate Leaf Melaleuca Leaf Oil, 5g of Scutellaria Baicalensis Extract and 0.25g of Citronella Oil, and dissolve it in a propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution with a concentration of 10% by weight. .

The above-mentioned compound Chinese herbal medicine antibacterial composition mixed liquid is added to a cleansing matrix (water, sodium hyaluronate, succinol, hydrolyzed collagen, lipids, etc.), and stirred to make a skin cream.

Comparative Example 1

Weighing the powder of Alternaria alternifolia powder with propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution with a concentration of 10% by weight.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Comparative Example 2

Weigh the mixed Chinese herbal medicine antibacterial composition mixed solution of the S. alternifolia leaf oil with propylene glycol solvent.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Comparative Example 3

Weigh the Scutellaria baicalensis extract with a propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution with a concentration of 10% by weight.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Comparative Example 4

The citronella oil was weighed and dissolved in a propylene glycol solvent to obtain a compound traditional Chinese medicine antibacterial composition mixed solution having a concentration of 10% by weight.

The above-mentioned compound traditional Chinese medicine antibacterial composition mixed liquid is added to a washing and protecting matrix (water, sodium hyaluronate, butanediol, hydrolyzed collagen and lipid, etc.), and the mixture is evenly mixed to prepare a skin cream.

Suspension quantitative sterilization test

The colonies of E. coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans which were cultivated for 18 to 24 hours were prepared by a direct colony suspension preparation method to prepare a bacteria suspension that meets the experimental requirements.

In a sterile test tube, add 1 ml of the test bacteria suspension and 4 ml of the compound traditional Chinese medicine antibacterial composition mixture prepared in Examples 1 to 5 and Comparative Examples 1 to 4, mix well, and keep the temperature constant to (20 ± 1 ° C) for 5 minutes and 10 minutes. . Take 0.5ml sample solution and add it to 4.5ml neutralizer (2% Tween-80 + 0.5% lecithin + 1% glycine + 0.03mol / L PBS) test tube, mix and neutralize for 10min. Take 1.0ml of the sample solution and measure the number of viable bacteria according to the live bacteria culture counting method to calculate the killing rate. If there are a large number of colonies growing on the plate, a serial 10-fold dilution can be performed and then counted for live bacteria culture. At the same time, the diluted liquid was used in place of the mixed liquid of the traditional Chinese medicine antibacterial composition prepared in Examples 1 to 5 and Comparative Examples 1 to 4, and a parallel test was performed as a positive control. The results are as follows:

Example 1

Example 2

Example 3

Example 4

Example 5

Comparative Example 1

Comparative Example 2

Comparative Example 3

Comparative Example 4

As can be seen from the data in the table, compared with the individual components, the compound Chinese herbal medicine antibacterial composition provided in Examples 1 to 5 used the specific ratios of Alternate Leaf Melaleuca Leaf Powder, Alternate Leaf Melaleuca Leaf Oil, Scutellaria Baicalensis The extract and citronella oil cooperate with each other and have a more obvious killing effect on pathogenic bacteria such as Escherichia coli, Staphylococcus aureus, Candida albicans and Pseudomonas aeruginosa. The compound traditional Chinese medicine antibacterial composition provided in Example 5 After 10 minutes, the sterilization rate is ≧ 99.9%.

Determination of the minimum inhibitory concentration (MIC value) of Staphylococcus aureus

Test method: The minimum inhibitory concentration (MIC) is determined by using the micro-dilution method, using the EP tube for comparative dilution, and diluting the mixed traditional Chinese medicine antibacterial composition mixed solution prepared in Examples 1 to 5 and Comparative Examples 1 to 4 by 8 concentrations. 50%, 25%, 12.5%, 6.25%, 3.13%, 1.56%, 0.78%, 0.39%, 0.20%, 0.10% were added to the 96-well plate, 50 μl per well. Add the diluted Staphylococcus aureus bacterial solution (10 ⁶ cfu / ml) to the wells of the compound traditional Chinese medicine antibacterial composition mixed solution prepared in Examples 1 to 5 and Comparative Examples 1 to 4, 50ul per well, and cover Incubate at 35 ° C for 24h. Observation with the naked eye, the lowest concentration of the drug tube without bacteria growth, that is, the MIC value of the test bacteria. The results are as follows:

As can be seen from the data in the table, compared with the individual components, the compound Chinese herbal medicine antibacterial composition provided in Examples 1 to 5 used the specific ratios of Alternate Leaf Melaleuca Leaf Powder, Alternate Leaf Melaleuca Leaf Oil, Scutellaria Baicalensis The extract and citronella oil cooperated with each other, and the minimum inhibitory concentration on S. aureus was significantly reduced.

Teen Facial Acne Treatment Test

Test subjects: 14 to 20 years old with facial acne. There were 25 cases in each test group.

Test method: The test is carried out for 14 days. Every morning and evening, 3 to 5 g of the skin cream prepared in Examples 1 to 5 and Comparative Examples 1 to 4 is applied to the face with acne.

Test criteria: ① healed: acne disappeared; ② markedly effective: acne reduced to a little; ③ effective: acne reduced; ④ ineffective: no effect on acne.

The effect of treatment test is shown in the table below.

sample	get well	Marked effect	effective	invalid	Recovery rate	Total efficiency
Example 1	8	7	3	7	32%	72%
Example 2	11	4	4	6	44%	76%
Example 3	15	2	5	3	60%	88%
Example 4	13	6	3	3	52%	88%
Example 5	15	6	3	1	60%	96%
Comparative Example 1	1	5	6	13	8%	48%
Comparative Example 2	3	5	6	11	12%	56%
Comparative Example 3	0	2	7	16	0	36%
Comparative Example 4	0	0	10	15	0	40%

As can be seen from the data in the table, compared with the comparative examples 1 to 4, the skin cream provided in Examples 1 to 5 has a relatively obvious effect of eliminating acne. The skin cream provided in Example 5 has the best effect, which illustrates the present invention. The compound Chinese medicine antibacterial composition with preferred component content has better effect. During the testing of the product of the present invention, no toxic or side effects were found, no allergic reaction occurred, and only 3 cases of localized skin ulceration had a slight tingling sensation during the first two days of use, and disappeared after the third day.

In the above-mentioned compound Chinese medicine antibacterial composition, each component is matched according to a specific ratio, and a variety of functional ingredients are rich in harmony and synergy with each other, and play a role in inhibiting pathogenic microorganisms, balancing the surface flora of the skin, isolating and removing harmful substances on the skin and Strengthen the nutritional effect on skin cells.

The above-mentioned compound traditional Chinese medicine antibacterial composition, the selected components are all natural plant ingredients, green and environmental protection, no toxic and side effects, mild and non-irritating.

The technical features of the embodiments described above can be arbitrarily combined. In order to simplify the description, all possible combinations of the technical features in the above embodiments have not been described. However, as long as there is no contradiction in the combination of these technical features, It should be considered as the scope described in this specification.

The above-mentioned embodiments only express several implementation manners of the present invention, and their descriptions are more specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be noted that, for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, which all belong to the protection scope of the present invention. Therefore, the protection scope of the invention patent shall be subject to the appended claims.

Claims (10)

1. A compound traditional Chinese medicine antibacterial composition, characterized by comprising, by weight parts, the following components:

   
2. The compound traditional Chinese medicine antibacterial composition according to claim 1, characterized by comprising, by weight parts, the following components:

   
3. The compound traditional Chinese medicine antibacterial composition according to claim 2, characterized by comprising, by weight parts, the following components:

   
4. The compound traditional Chinese medicine antibacterial composition according to any one of claims 1 to 3, wherein the Scutellaria baicalensis extract is prepared by the following method:

   Weigh the root bark of Scutellaria baicalensis and boil it with water, filter out the solids and obtain.
5. The compound traditional Chinese medicine antibacterial composition according to claim 4, characterized in that the method of boiling comprises: adding 250ml to 350ml of water for every 15g of Scutellaria baicalensis root bark, and boiling for 30min to 60min until the remaining liquid is 80ml to 120ml .
6. The compound traditional Chinese medicine antibacterial composition according to any one of claims 1 to 3, characterized in that the intergrowth leaf white lamellar leaf powder is prepared by the following method:

   Fresh branches with a single branch diameter of not more than 0.5 cm and the leaves thereon are selected, and dried, crushed and sieved to obtain it.
7. The compound traditional Chinese medicine antibacterial composition according to claim 6, characterized in that the screening uses a 60-180 mesh screen.
8. Application of the compound traditional Chinese medicine antibacterial composition according to any one of claims 1 to 7 in the preparation of toiletries.
9. A washing and care article, comprising a washing and care base, a solvent, and the compound Chinese medicine antibacterial composition according to any one of claims 1 to 7.
10. The preparation method of the toiletries according to claim 9, further comprising the following steps:

    The compound traditional Chinese medicine antibacterial composition is mixed with the solvent, and then added to the washing and protecting matrix.