CN111701003A - Application of Huoxiang Zhengqi compound in preparation of external washing and protecting product - Google Patents

Abstract

The invention discloses an application of a wrinkled giant hyssop compound in an external washing product, wherein the external washing product comprises a skin washing product and a clothes washing product, the skin washing product comprises shower gel, bath salt, perfumed soap, liquid soap and wash-free liquid soap, the clothes washing product comprises liquid soap and soap, the wrinkled giant hyssop compound comprises wrinkled giant hyssop decoction and a wrinkled giant hyssop preparation, the wrinkled giant hyssop preparation comprises wrinkled giant hyssop water, a wrinkled giant hyssop mixture, a wrinkled giant hyssop oral liquid and wrinkled gianthyssop particles, and the content of the wrinkled gianthyssop compound in the external washing product is 1-20 wt%. The functions of antibiosis, antivirus and resistance enhancement of the compound ageratum vital qi are exerted in an external use mode, and the problems of gastrointestinal irritation and liver burden existing in the compound ageratum vital qi oral administration are solved.

Description

Application of Huoxiang Zhengqi compound in preparation of external washing and protecting product

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to application of a compound of agastache rugosus and healthy energy in an external washing product.

Background

The compound Huoxiang Zhengqi is originated from Huoxiang Zhengqi powder in Song Dynasty's "Taiping Huimin He Ji Ju Fang, and the whole formula consists of Huoxiang, Magnolia officinalis, radix Angelicae Dahuricae, pericarpium Arecae, Perillae herba, pericarpium Citri Tangerinae, rhizoma Pinelliae Fermentata, Poria, radix Platycodi, Glycyrrhrizae radix and Atractylodis rhizoma. Has effects in relieving exterior syndrome, eliminating dampness, regulating qi-flowing, and regulating stomach, and can be used for treating common cold caused by affection of exogenous wind-cold, internal injury, damp stagnation, or summer-heat and dampness. The clinical practice and research in recent years show that the compound oral administration of agastache for restoring healthy energy can prevent and treat various virus infectious diseases including influenza and rotavirus. However, due to the relationship of medicine and dosage form, Huoxiang Zhengqi is not suitable for all people and it is impossible to prevent diseases by taking Huoxiang Zhengqi for a long time.

Along with the progress of society, the use frequency of daily chemical products is gradually increased, and washing products such as bath cream, perfumed soap, hand sanitizer, shampoo and the like become necessary products in daily life of people. However, the current external washing and caring products are mainly used for cleaning skin and have no effects of daily health care and disease prevention.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides the application of the compound of agastache rugosus and healthy qi in the external washing product, the using mode of the compound of agastache rugosus and healthy qi is added, the antibacterial, antiviral and resistance enhancing effects of the compound of agastache rugosus and healthy qi are exerted in an external mode, and the problems of gastrointestinal irritation and liver burden existing in the compound of agastache rugosus and healthy qi when the compound of agastache rugosus and healthy qi is orally taken are solved.

In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:

the application of HUOXIANGZHENGQI Compound in preparing external washing product is provided.

The beneficial effects produced by adopting the scheme are as follows: the compound agastache healthy energy is mainly prepared from agastache rugosus, perilla leaves, angelica dahurica, bighead atractylodes rhizome stir-fried with bran, dried orange peel, ginger processed pinellia tuber, ginger processed magnolia bark, poria cocos, platycodon grandiflorum, liquorice, pericarpium arecae, Chinese date and ginger, and has antibacterial and antiviral effects when the compound agastache healthy energy is externally applied, and the compound agastache healthy energy is added into a washing and protecting product, wherein the effective components can kill viruses and bacteria on the surface of the skin, reduce the damage of the bacteria and the viruses to a human body, enter the body through skin striae, enhance the transdermal absorption of the effective components, promote the direct blood access of the effective components, improve the bioavailability of the medicine and realize the effect of enhancing.

Most viruses need an acidic environment from the beginning of replication to the maturation of virus particles to the later assembly process, the external washing and caring product contains a certain amount of free alkali, the alkaline environment of the washing and caring product can inhibit the growth of the viruses to a certain extent, and after the effective components with antibacterial and antiviral effects are added into the washing and caring product, the antibacterial and antiviral effects are remarkably improved through the synergistic effect between the free alkali and the medicinal components.

Further, topical personal care products include skin care products and garment care products.

Further, skin care products include body washes, bath salts, soaps, hand washes, and leave-on hand washes.

The beneficial effects produced by adopting the scheme are as follows: when the compound agastache rugosus healthy qi lotion is used, the soap base component can remove oil stains on the surface of the skin, increase the hydration of the skin, cause the cuticle to swell and loosen, reduce the compactness of tissues, form gaps, promote the diffusion and storage of medicinal components in the cuticle, simultaneously, hot water can promote the skin striae to be opened to form a medicinal channel, so that the medicinal effective components can permeate into the skin and directly enter the blood to play a role in enhancing the immunity.

Moreover, although the compound of agastache rugosus healthy qi is a Chinese patent medicine and has better effect, the compound of agastache rugosus healthy qi is not suitable for all people due to the poor taste, the irritation to the digestive tract, certain burden on the liver and other factors, and is added into an external washing and nursing product, and the compound of agastache rugosus healthy qi is permeated into the skin in an external mode and directly enters the blood, so that the bioavailability of the medicine is improved, the irritation to the gastrointestinal tract and the inactivation of the first pass effect of the liver on the medicine are reduced, and the medication way of the compound of agastache rugosus healthy qi for preventing and treating viral diseases is; the skin washing and caring product can be used for a long time without any side effect.

Further, laundry care products include laundry detergents and soaps.

The beneficial effects produced by adopting the scheme are as follows: the compound agastache rugosus healthy energy is added into the clothes washing and protecting product, so that the sterilization and disinfection effects on the clothes can be improved, and the damage of bacteria and viruses on the clothes to a human body can be reduced.

Furthermore, the compound of wrinkled giant hyssop comprises a decoction and a preparation, wherein the preparation comprises a mixture, an oral liquid and a granule.

Furthermore, the content of the ageratum vital qi compound in the external washing and nursing product is more than 1 wt% -20 wt%.

Furthermore, the content of the ageratum vital qi compound in the skin washing and protecting product is 1.6 to 3 weight percent.

Furthermore, the content of the ageratum vital qi compound in the skin washing and protecting product is 1.6 wt%.

Furthermore, the content of the ageratum vital qi compound in the clothing washing and protecting product is 16 wt%.

The beneficial effects produced by adopting the scheme are as follows: the content of the compound of agastache rugosus healthy energy is too low, the content of active ingredients is low, and the effect of enhancing immunity by synergistically inhibiting bacteria, resisting viruses and the like cannot be achieved. The compound content of the ageratum vital qi is high, which can inhibit the growth of normal symbiotic microorganisms on the surface of skin and influence the health of human body, therefore, the content of the compound of the ageratum vital qi in the washing and nursing product is controlled within a certain range, and the bacteriostatic and antiviral effects are best.

The invention has the beneficial effects that:

1. the prepared external washing product has the functions of resisting bacteria and viruses and enhancing the immunity of a human body, wherein the compound component of the agastache rugosus vital qi can be used for cooperating with the washing product to enhance the antibacterial and antiviral effects, and meanwhile, the compound component of the agastache rugosus vital qi can enter the blood through skin striae to play a role in enhancing the resistance.

2. The compound of agastache rugosus healthy qi is added into the washing and nursing product, so that a new use mode is provided for the compound of agastache rugosus healthy qi, the compound of agastache rugosus healthy qi is more suitable for people, intestinal irritation caused by oral administration of the compound of agastache rugosus healthy qi is avoided, meanwhile, the inactivation of the first pass effect of the liver on medicinal components is reduced through a transdermal absorption mode, and the exertion of the medicinal effect is promoted.

Detailed Description

Example 1

The application of HUOXIANGZHENGQI oral liquid in preparing soap is provided. The content of the ageratum zhengqi oral liquid in the perfumed soap is 20 percent by weight.

The preparation method of the soap comprises the following steps: (1) taking a soap base, and heating and melting;

(2) adding the Huoxiang Zhengqi oral liquid into the dissolved soap base, and uniformly mixing to obtain a mixture;

(3) pouring the mixture into a mold, and cooling to obtain the product.

Example 2

Application of HUOXIANGZHENGQI liquid in preparing bath lotion is provided. The content of the ageratum vital essence liquid in the shower gel is 1.6 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches vital qi water and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Example 3

Application of HUOXIANGZHENGQI oral liquid in preparing bath lotion is provided. The content of the ageratum zhengqi oral liquid in the shower gel is 1.6 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches vital essence oral liquid and aromatic into reaction kettle, stirring for 30 min, and making into final product.

Example 4

Application of HUOXIANGZHENGQI mixture in preparing bath lotion is provided. The content of the ageratum vital energy mixture in the shower gel is 1.6 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches mixture and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Example 5

Application of HUOXIANGZHENGQI granule in preparing bath lotion is provided. The content of the ageratum vital qi granules in the shower gel is 1.6 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches vital qi granule and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Example 6

Application of HUOXIANGZHENGQI mixture in preparing hand lotion is provided. The content of the ageratum vital energy mixture in the hand sanitizer is 10 percent by weight.

The preparation method of the hand sanitizer comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches mixture and aromatic into reaction kettle, and stirring for 30 min.

Example 7

Application of HUOXIANGZHENGQI liquid in preparing soap is provided. The content of the ageratum zhengqi liquid in the perfumed soap is 3 wt%.

The preparation method of the soap comprises the following steps: (1) taking a soap base, and heating and melting;

(2) adding herba Agastaches vital qi water into dissolved soap base, and mixing to obtain mixture;

(3) pouring the mixture into a mold, and cooling to obtain the product.

Comparative example 1

Application of HUOXIANGZHENGQI liquid in preparing bath lotion is provided. The content of the ageratum vital essence liquid in the shower gel is 0.5 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches vital qi water and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Comparative example 2

Application of HUOXIANGZHENGQI liquid in preparing bath lotion is provided. The content of the ageratum vital qi liquid in the shower gel is 35 percent by weight.

The preparation method of the shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic, herba Agastaches vital qi water and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Reference 3

The preparation method of the common shower gel comprises the following steps: placing a natural thickener and water in a reaction kettle, stirring, heating to 75 ℃ and dissolving, stirring, cooling and cooling to 55 ℃; adding natural oil, natural antiseptic and aromatic into reaction kettle, stirring for 30 min to obtain the final product.

Further, the natural thickener and the natural oil and fat in the above examples. The natural preservatives, fragrances and soap bases are the same.

Test examples

Bacteriostatic effect of washing and protecting articles

1. Preparing a bacterial suspension: taking a proper amount of staphylococcus aureus, escherichia coli, bacillus subtilis and aerobacter strains under the aseptic condition, culturing in a liquid culture medium, and placing in a constant-temperature shaking table machine for later use.

2. Preparation of beef extract peptone medium: accurately weighing 3g of beef extract, 10g of peptone and 5g of sodium chloride, putting the beef extract, a small amount of distilled water into a beaker, heating on an asbestos net to completely dissolve the beef extract, adding 15g of agar, continuously heating to dissolve the agar, finally supplementing lost water, adding a small amount of 1mol/L sodium hydroxide to adjust the pH value to 7.6, subpackaging the beef extract in conical flasks as required, plugging and bundling the beef extract, and sterilizing at high temperature for later use.

Taking the washing and caring products in the examples 1-7 and the comparative examples 1-3, respectively, fully dissolving the washing and caring products in sterile water, respectively diluting to 200 μ g/ml, and preparing into medicated filter paper sheets, wherein the positive control group is 1ml/ml penicillin, the negative control group is 85% ethanol, performing bacteriostatic determination experiment, determining bacteriostatic ring size, and determining bacteriostatic ring diameter and minimum bactericidal concentration, and the results are shown in table 1:

table 1: bacteriostatic test effect of washing and caring articles

The data in the table show that the diameters of the inhibition zones in examples 1-7 and comparative example 2 are larger than that of the negative control group, and the washing product containing the agastache rugosus vital qi strengthening compound has stronger inhibition effect on staphylococcus aureus, aerobacter aerogenes, escherichia coli and the like; comparing the data in examples 2-5, it can be seen that the bacteriostatic action of the Huoxiang Zhengqi liquid in example 2 on Staphylococcus aureus, Aerobacter aerogenes and Escherichia coli is stronger than that of other preparations. By comparing the data in example 1, example 2, example 6, example 7, comparative example 1, comparative example 2 and comparative example 3, it can be seen that the higher the concentration of the traditional Chinese medicine compound in the washing and caring product is, the stronger the bacteriostatic action on staphylococcus aureus, aerobacter aerogenes, escherichia coli and the like is.

Second, the washing and protecting product can enhance the immunity of human body

Searching 100 experimenters with poor resistance and susceptible to cold in winter, randomly dividing the experimenters into 10 groups, recording the number of times of cold in the last year and the time of cold in each group of 10 experimenters, calculating the average number of cold in each group and the average healing time in each group, respectively using the washing products in the examples 1-7 and the comparative examples 1-3 for the second year, recording and calculating the average number of cold in each group and the average healing time in each group in the year, and specifically finding the result in table 2:

table 2: cold condition statistical table

As can be seen from the data in the table, after the experimenters in the groups of examples 1-7 and comparative examples 1-2 continuously use the corresponding washing products, the frequency and the time of cold are reduced, especially the proportion of the reduction of the frequency and the time of cold of the patients in the group of example 2 is larger, and the washing products in the example 2 are proved to have better resistance enhancing effect. Comparing the data in example 2 and example 5, it can be seen that the effect of enhancing the resistance of Huoxiang Zhengqi liquid is the best. From the data in example 1, example 2, example 6 and example 7, it can be known that although the concentration of the mixture of agastache rugosa and vital energy essence in the washing product in example 1 and example 7 is higher, the effect of enhancing the resistance is worse than that in example 2, which proves that the concentration of the mixture of agastache rugosa and vital energy essence in the washing product is too high to affect the growth and development of beneficial microorganisms on the surface of the human body and reduce the resistance of the human body, therefore, the content of the mixture of agastache rugosa and vital energy essence in the washing product is not higher.

Third, antiviral effect of washing and caring product

The Huoxiang Zhengqi Fufang has the main effects of relieving exterior syndrome, eliminating dampness, regulating qi and regulating the middle warmer, and is used for treating cold caused by wind cold and internal injury and damp stagnation. The gastrointestinal type cold is mainly gastrointestinal dysfunction caused by virus infection, the Huoxiang Zhengqi prescription has antiviral effect when being taken orally, has definite curative effect when being clinically applied to the gastrointestinal type viral cold, and has obvious improvement effect on symptoms such as nausea or vomiting, diarrhea, fever and the like. The main pathogenic viruses of gastrointestinal influenza are rotavirus (HRV) and coxsackie virus (Cox.V). In the research, a diluent prepared by the ageratum vital essence shower gel is selected as an intervention measure, ribavirin is adopted as a positive control drug, a Reed-Muench formula is used for calculating research indexes, and the effect and the safety of the diluent on resisting the two viruses are observed through the inhibition effect of the diluent on laryngeal cancer cells (Hep-2) and Romonkey kidney cells (MA104) after virus infection.

1. Material

1.1 medicine: the body wash of example 2; ribavirin: 100 mg/tablet, North China pharmaceutical group, Inc. (approved article No.: 080646).

1.2 instruments and devices: MK3 full-automatic enzyme labeling instrument (Finland), SW-CJ-IF super clean bench, CO2 incubator, TD5 bench multi-tube rack low speed centrifuge, XS-200 optical microscope, XSB-1A biological inverted microscope. RPMI1640 cell culture and maintenance fluids, fetal bovine serum, 0.25% pancreatin, MTT were all supplied by Changshaoxin BioCorp.

1.3 cell and viral strains: the laryngeal cancer cell line (Hep-2) and Romonkey kidney cell (MA104) were provided by the Hunan Yao college of medicine cell center of the university in Central south China and the institute of disease control, respectively. HRV was provided by the Chinese institute for disease control, Cox. VA-16 was provided by the clinical laboratory center in Sichuan province.

2. Method of producing a composite material

2.1 the Huoxiang Zhengqi bath lotion of example 2 was diluted to 1mg/ml with sterile double distilled water for further use.

2.2 determination of toxicity of the ageratum vital essence shower gel on in vitro culture cells: dissolving herba Agastaches vital qi bath lotion with sterile double distilled water to 1mg/ml, diluting with cell maintaining liquid (1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:125, 1:256), adding diluted herba Agastaches vital qi bath lotion into full monolayer cultured cells, setting 3 multiple wells per drug mass concentration, 100 μ l per well, setting normal cell control, and controlling at 37 deg.C and 5% CO₂Culturing in an incubator, and observing cytopathic effects (CPE) such as cell fusion, necrosis, desquamation, floating, death and the like under an inverted microscope; the representation method comprises the following steps: 0-25% (+), 25-50% (++), 50-75% (+++), 75-100% (+++), when CPE is not advanced, using trypan blue to stain, counting living cells, using MTT colorimetric method to measure OD value at 492nm, calculating cell inhibition rate, then calculating half Toxic Concentration (TC) of medicine according to Reed-Muench method₅₀) Taking the mass concentration of the medicine with the cell survival rate more than 80 percent as the maximum nontoxic concentration (TC) of the medicine to the cells₀) And performing an antiviral experiment.

Cell survival (%) - (mean OD value of drug group/mean OD value of normal cell control group)/100% cell inhibition (%) - (100% -cell survival rate)

2.3 half the infection dose (TCID) of HRV, Cox.V on cultured cells₅₀) The HRV and Cox.V are diluted to 1 × 10 according to the concentration gradient^-1～1×10^-9Respectively inoculating virus solution with various dilutions into cultured confluent monolayer cells according to 100 μ L/well, adsorbing 1H at 37 deg.C, taking out, adding cell maintenance solution (each virus dilution is provided with 3 wells) at 37 deg.C and 5% CO₂Culturing in incubator, replacing cell maintenance solution at 48H intervals, observing CPE under inverted microscope every day, taking out cultured cells after 7D observation, adding MTT20 μ L (5mg/ml), 37 deg.C, and 5% CO per well₂Culturing for 4h in an incubator, discarding supernatant in the wells, adding 150 μ L of dimethyl sulfoxide into each well, shaking for 10min at 100 times/min, measuring absorbance (A) value with an enzyme-labeling instrument 492nm, setting 630nm as reference wavelength, calculating average A value, and calculating virus TCID by Reed-Muench formula₅₀Results show TCID of HRV₅₀TCID of 1 × 10-4.6, Cox.V₅₀Is 1 × 10-5.3.

2.4 antiviral test of HUOXIANGZHENGQI bath lotion, 100 μ l/well of monolayer cells of 96-well culture plate were added to 100 × TCID₅₀HRV virus solution and 100 × TCID₅₀The COV.V.A-16 virus liquid. Standing at 37 deg.C for 5% CO₂Adsorbing for 2h in the incubator. Discarding the virus solution, washing twice with serum-free culture solution (RPMI-1640), and adding diluted drugs with different concentrations (not exceeding TC)₀Concentration) and ribavirin working solution, each drug was replicated in 3 wells at different dilutions. Viral controls and normal cell controls were set up in the experiment. Each plate was placed at 37 ℃ in 5% CO₂Incubations were continued and CPE was observed once daily. When the CPE of the virus control group cells reaches ++++ - +++++, the OD value is measured by adopting an MTT method, the virus inhibition rate is calculated according to the following formula, and the 50% inhibition concentration of cytopathic effect (IC 50) of the medicine is calculated according to a Reed-Muench method, and the formula of the Therapeutic Index (TI) is shown as follows: TI being TC₅₀/IC₅₀And calculating the therapeutic index of the medicine.

The virus inhibition ratio (%) × (average OD value of drug group-average OD value of virus control group)/(average OD value of control group-average OD value of virus control group) × 100%.

3. Results

3.1 cytotoxicity of drugs

The normal control wells were morphologically normal during the test period. The toxicity of the drug on two cells is consistent, the toxicity is manifested by cytopathic effect (CPE) such as cell fusion, necrosis, desquamation, floating, death, etc., the optical density value of the MTT method detector is obviously reduced, and the cell TC₅₀And TC₀The results are shown in Table 3;

table 3: drug-on-cell TC₅₀And TC₀(n＝3)

3.2 antiviral Effect of drugs

3.2.1 inhibition of Cox.V A-16 by Huoxiang Zhengqi shower gel: as can be seen from Table 4, the antiviral effects of the test substances show a dose-effect relationship, i.e., as the mass concentration of the drug increases, the antiviral effects also increase.

Table 4: inhibition of wrinkled giant hyssop vital energy shower gel on Cox.V A-16 (n is 3)

3.2.2 inhibition of Huoxiang Zhengqi bath lotion on HRV: as can be seen from Table 5, the antiviral effects of the test substances show a dose-effect relationship, i.e., the antiviral effects increase with increasing drug mass concentration.

Table 5: inhibition of HRV by wrinkled giant hyssop vital energy shower gel liquid soap (n is 3)

Claims (10)

1. The application of HUOXIANGZHENGQI Compound in preparing external washing product is provided.

2. The use of claim 1, wherein the external care device comprises a skin care device and a garment care device.

3. The use of claim 1, wherein the skin cleansing product comprises body wash, bath salt, soap, hand sanitizer, and leave-on hand sanitizer.

4. The use of claim 1, wherein the laundry care product comprises a laundry detergent and soap.

5. The use of claim 2, wherein the HUOXIANGZHENGQI compound comprises HUOXIANGZHENGQI decoction and HUOXIANGZHENGQI preparation, and the HUOXIANGZHENGQI preparation comprises HUOXIANGZHENGQI liquid, HUOXIANGZHENGQI mixture, HUOXIANGZHENGQI oral liquid and HUOXIANGZHENGQI granule.

6. The use according to any one of claims 1 to 4, wherein the content of the Huoxiang Zhengqi Fufang in the external use washing product is 1 wt% to 20 wt%.

7. The use according to any one of claims 1 to 4, wherein the amount of the Huoxiang Zhengqi Fufang in the skin care product is 1.6 to 3 wt%.

8. The use according to claim 7, wherein the content of the Huoxiang Zhengqi Fufang in the skin care product is 1.6 wt%.

9. The use according to claim 6, wherein the amount of the Huoxiang Zhengqi Fufang in the laundry article is 16 wt%.

10. A topical lotion comprising the wrinkled giant hyssop vital energy compound of any one of claims 1-9.