CN105012201B - The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed - Google Patents

The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed Download PDF

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CN105012201B
CN105012201B CN201510503560.3A CN201510503560A CN105012201B CN 105012201 B CN105012201 B CN 105012201B CN 201510503560 A CN201510503560 A CN 201510503560A CN 105012201 B CN105012201 B CN 105012201B
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chinese hawthorn
hawthorn seed
alcohol extract
helicobacter pylori
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严杰
林世恩
葛玉梅
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HANGZHOU HUAZHOU TECHNOLOGY Co Ltd
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HANGZHOU HUAZHOU TECHNOLOGY Co Ltd
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Abstract

The invention discloses a kind of oral care products with anti-helicobacter pylori of alcohol extract containing Chinese hawthorn seed, it is characterised in that the oral care product includes Chinese hawthorn seed alcohol extract.The present invention provides a kind of Chinese Traditional Medicine oral care product, and drug extracting method is simple, and dosage ingredient stability can directly go deep into oral cavity.Long-time service will not cause internal environment of oral cavity disorderly, without side-effects.The present invention not only has the similar effect for more having killing helicobacter pylori except halitosis, treating dental ulcer, anti-oral cavity common pathogen reach anti-inflammatory purpose, helicobacter pylori effectively being prevented orally to propagate of the oral cares class product such as existing herbal health care toothpaste.

Description

The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed
Technical field
The invention belongs to chemical product technical fields, and in particular to a kind of alcohol extract containing Chinese hawthorn seed has anti-helicobacter pylori The oral care product of effect.
Background technology
Helicobacter pylori, English name Helicobacter pylori, abbreviation Hp.Helicobacter pylori is a kind of infection rate, biography The very strong pathogenic bacteria of metachromia.Australian scholar's Barry Marshall Barry J.Marshall and Luo Bin Warrens in 1983 J.Robin Warren for the first time in Gastritis Mucosa Biopsy tissue separation detection to helicobacter pylori.Australia is big within 1989 Leah scholar Krajden detects HP out of Gastritis Buccodental plaque, and from molecular level confirm its in stomach Hp is identical.Hp is a kind of infectious disease orally propagated, and oral cavity Hp is the important depots of stomach Hp.Helicobacter pylori is to human body The damage of health is great:Be induce gastric cancer the first kind it is carcinogenic;90% duodenal ulcer and 80% gastric ulcer, by Helicobacter pylori infections cause.In China chronic active gastritis patient, the recall rate of helicobacter pylori is 95% to 100% ;Recall rate is 85% to 95% in duodenal ulcer patients;Recall rate is 70% to 80% in patients w ith peptic ulcer disease;Gastric cancer is suffered from Recall rate is 80% in person.Epidemiological survey China 12 years old or less teenager's Hp infection rates are up to 40%, adult's Hp infection Rate is up to 60% or more.Existing medical procedures are taken using large dosage of Multiple Classes of Antibiotics and kill helicobacter pylori simultaneously.Existing skill The shortcomings that art is:1. killing a large amount of beneficial bacteriums, taking for the large dosage of Multiple Classes of Antibiotics of disease recurrent exerbation 2. is easily led to, is increased The drug resistance of helicobacter pylori, bacterium, which generates drug resistance, makes the therapeutic effect of effective antibiotic originally reduce or lose, and increases The treatment difficulty and medical treatment cost of patient disease are added.
It as the saying goes, illness finds its way in by the mouth, and the infectious disease that direct oral cavity is propagated is other than helicobacter pylori, also Escherichia coli, golden yellow Color staphylococcus and candida albicans bacterium etc., therefore, oral hygiene and its important, and the nursing of oral hygiene, it is most important just It is to add disinfectant mostly using the product of toothpaste, mouthwash etc, existing oral care product to administer mouth disease, But commonly using the drug resistance that can increase oral bacteria, destroy oral environment.
Hawthorn(Scientific name:Crataegus pinnatifida), and hawthorn, large-fruited Chinese hawthorn, hawthorn, large-fruited Chinese hawthorn fruit, acid Jujube, red fruit, red fruit are rose family hawthorns, native to China, are distributed in Shandong, Henan, Shanxi, Hebei, Liaoning etc. Area.Hawthorn has important medical value, and slightly warm in nature is sour sweet, enters spleen, stomach, Liver Channel, has food digesting stomach fortifying, activates blood circulation and disperses blood clots, restrains The function of stop dysentery.Since ancient times, just become the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting.
Chinese hawthorn seed is the seed of rosaceous plant hawthorn or fructus crataegi cuneatae etc..《Detailed outline》In recorded that " helping digestion that gulps down mill product, is controlled The effect of insane hernia ".《The new compilation of materia medica》In recorded " control hernia, expedite the emergence of " purposes effect.《The southern regions of the Yunnan Province book on Chinese herbal medicine》Described in " Chinese hawthorn seed seven Seven, Plant Soot is clothing, and wine is swallowed, and controls difficult labour ".Modern study finds, the extract in Chinese hawthorn seed have sterilization, it is antiviral, The effects that anti-oxidant, reducing blood lipid, shield tooth, treatment breathing problem.The exploitation of hawthorn is more at present, and is easy to the Chinese hawthorn seed abandoned Exploitation it is less, be not fully used, cause the waste of resource, and the extract of Chinese hawthorn seed is produced for oral care In product, there is not yet report.
Toothpaste be in daily life daily must use dental care products, with the improvement of living standards mouthwash, chewing gum, Candy, effervescent tablet or mouth sprays application are also more and more extensive, the oral cares class product such as presently commercially available common health-care toothpaste Most of to make antimicrobial component using synthesis chemicals, synthetic antibacterial agents are used for a long time, and to human body, there are potentially hazardous and safe Sex chromosome mosaicism.Although having some herbal health care toothpaste in the market, antibacterial effect is also not ideal enough, does not have especially and kills Helicobacter pylori activity.
Invention content
For problems of the prior art, what the present invention provided a kind of alcohol extract containing Chinese hawthorn seed has anti-helicobacter pylorus The oral care product of bacterium effect.
The present invention is realized by the following technical programs:
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that should Oral care product includes Chinese hawthorn seed alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute It is toothpaste, mouthwash, chewing gum, candy, effervescent tablet or mouth sprays to state oral care product.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Toothpaste is stated to be grouped as by the group of following parts by weight:80-100 parts of calcium monohydrogen phosphate, 20-30 parts of water, 5-10 parts of D-sorbite, 1-5 parts of hydrated silica, 1-5 parts of polyethylene glycol-8,1-5 parts of propylene glycol, 1-5 parts of laruyl alcohol sodium sulfovinate, cellulose gum 1- 5 parts, 1-5 parts of hydroxyethyl cellulose, 1-5 parts of essence, 1-5 parts of sodium benzoate, 1-5 parts of saccharin sodium, Chinese hawthorn seed alcohol extract 1- 5 parts.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Mouthwash is stated to be grouped as by the group of following parts by weight:90-100 parts of water, 10-20 parts of sorbierite, 5-10 parts of propylene glycol, wood 5-10 parts of sugar alcohol, 5-10 parts of PEG-400 polyethylene glycol, 1-5 parts of glycyrrhizic acid diformazan, 1-5 parts of sodium benzoate, essence 1-3 Part, 1-5 parts of Chinese hawthorn seed alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Chewing gum is stated to be grouped as by the group of following parts by weight:30-40 parts of xylitol, 20-30 parts of matrix, 15-20 parts of sorbierite, 10-15 parts of maltitol liquor, 5-10 parts of D-mannital, 150-200 parts of citric acid, 00-120 parts of phosphatidase 1, Aspartame 30-40 parts, 30-40 parts of essence, 1-5 parts of Chinese hawthorn seed alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Candy is stated to be grouped as by the group of following parts by weight:50-100 parts of maltitol, 1-5 parts of citric acid, 1-5 parts of essence, hawthorn 1-5 parts of core alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Effervescent tablet is stated to be grouped as by the group of following parts by weight:20-30 parts of VC, 10-20 parts of citric acid, 30-40 parts of sodium bicarbonate, 5-10 parts of sodium carboxymethyl starch, 10-20 parts of lactose, 1-5 parts of Aspartame, 1-5 parts of essence, 1-5 parts of PVP-K30, 1-5 parts of PEG6000,1-5 parts of superfine silica gel powder, 1-5 parts of magnesium stearate, 1-5 parts of Chinese hawthorn seed alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Mouth sprays are stated to be grouped as by the group of following parts by weight:40-50 parts of water, 5-10 parts of sorbierite, 5-10 parts of propylene glycol, wood 1-5 parts of sugar alcohol, 1-5 parts of PEG-400 polyethylene glycol, 1-5 parts of glycyrrhizic acid diformazan, 1-5 parts of sodium benzoate, 1-2 parts of essence, 1-5 parts of Chinese hawthorn seed alcohol extract.
The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that institute Chinese hawthorn seed alcohol extract is stated to prepare using following methods:
1)Clean Chinese hawthorn seed after drying is taken, crushes, obtains Chinese hawthorn seed powder;
2)By solid-liquid ratio(g/ml)1:10 ratio, in step 1)The ethyl alcohol of addition 70% in obtained Chinese hawthorn seed powder, 80 DEG C Temperature condition under reflux extracting 4h, obtain extracting solution;
3)Extracting solution filter paper is filtered under diminished pressure, and is obtained a filtered solution, is added and step 2 in filter residue)70% second of same volume Alcohol, by step 2)Method extract filtering and removing slag after 2h again, obtain secondary filtered solution;
4)Merge a filtered solution and secondary filtered solution, with spin concentration under conditions of Rotary Evaporators 35r/min, 60 DEG C To paste or powdery;
5)Yield is calculated, 4 DEG C of refrigerators save backup.
The present invention provides a kind of Chinese Traditional Medicine oral care product, and drug extracting method is simple, dosage ingredient stability, can Directly go deep into oral cavity.Long-time service will not cause internal environment of oral cavity disorderly, without side-effects.The present invention not only has existing Chinese herbal medicine to protect The oral cares class product such as toothpaste is similar except halitosis, treating dental ulcer, anti-oral cavity common pathogen reach anti-inflammatory purpose, more There is killing helicobacter pylori, the effect for effectively preventing helicobacter pylori from orally propagating.
Specific implementation mode
Below in conjunction with specific embodiment, invention is further described in detail, and provides specific implementation mode.
Embodiment 1
The toothpaste with anti-helicobacter pylori of the alcohol extracting containing Chinese hawthorn seed, is grouped as by the group of following parts by weight: 90 parts of calcium monohydrogen phosphate, 25 parts of water, 7 parts of D-sorbite, 3 parts of hydrated silica, 3 parts of polyethylene glycol-8,4 parts of propylene glycol, the moon 2 parts of cinnamic alcohol sodium sulfovinate, 3 parts of cellulose gum, 3 parts of hydroxyethyl cellulose, 3 parts of essence, 3 parts of sodium benzoate, 3 parts of saccharin sodium, 3 parts of Chinese hawthorn seed alcohol extract.
Wherein, Chinese hawthorn seed alcohol extract is prepared using following methods:
1)Clean Chinese hawthorn seed after drying is taken, crushes, obtains Chinese hawthorn seed powder;
2)By solid-liquid ratio(g/ml)1:10 ratio, in step 1)The ethyl alcohol of addition 70% in 100g Chinese hawthorn seed powder is made 1000mL, reflux extracting 4h, obtains extracting solution under 80 DEG C of temperature condition;
3)Extracting solution filter paper is filtered under diminished pressure, and is obtained a filtered solution, 70% ethyl alcohol of 1000mL is added in filter residue, by step 2)Method extract filtering and removing slag after 2h again, obtain secondary filtered solution;
4)Merge a filtered solution and secondary filtered solution, with spin concentration under conditions of Rotary Evaporators 35r/min, 60 DEG C To paste or powdery;
5)Yield is calculated, 4 DEG C of refrigerators save backup
Chinese hawthorn seed alcohol extract recovery rate=(Chinese hawthorn seed alcohol extract is evaporated object weight/Chinese hawthorn seed coarse powder weight)×100%.
Embodiment 2
The mouthwash with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed is grouped by the group of following parts by weight At:95 parts of water, 15 parts of sorbierite, 7 parts of propylene glycol, 8 parts of xylitol, 8 parts of PEG-400 polyethylene glycol, glycyrrhizic acid diformazan 3 Part, 3 parts of sodium benzoate, 2 parts of essence, 3 parts of Chinese hawthorn seed alcohol extract.
The preparation method is the same as that of Example 1 for Chinese hawthorn seed alcohol extract.
Embodiment 3
The chewing gum with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed is grouped by the group of following parts by weight At:35 parts of xylitol, 25 parts of matrix, 17 parts of sorbierite, 17 parts of maltitol liquor, 8 parts of D-mannital, 170 parts of citric acid, 10 parts of phosphatidase 1,35 parts of Aspartame, 35 parts of essence, 3 parts of Chinese hawthorn seed alcohol extract.
The preparation method is the same as that of Example 1 for Chinese hawthorn seed alcohol extract.
Embodiment 4
The candy with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, is grouped as by the group of following parts by weight: 75 parts of maltitol, 3 parts of citric acid, 3 parts of essence, 3 parts of Chinese hawthorn seed alcohol extract.
The preparation method is the same as that of Example 1 for Chinese hawthorn seed alcohol extract.
Embodiment 5
The effervescent tablet with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed is grouped by the group of following parts by weight At:VC25 parts, 15 parts of citric acid, 35 parts of sodium bicarbonate, 7 parts of sodium carboxymethyl starch, 15 parts of lactose, 3 parts of Aspartame, essence 3 parts, 3 parts of 3 parts of PVP-K30, PEG6000,3 parts of superfine silica gel powder, 3 parts of magnesium stearate, 3 parts of Chinese hawthorn seed alcohol extract.
The preparation method is the same as that of Example 1 for Chinese hawthorn seed alcohol extract.
Embodiment 6
The mouth sprays with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed are grouped by the group of following parts by weight At:45 parts of water, 8 parts of sorbierite, 7 parts of propylene glycol, 3 parts of xylitol, 3 parts of PEG-400 polyethylene glycol, glycyrrhizic acid diformazan 2 Part, 3 parts of sodium benzoate, 2 parts of essence, 3 parts of Chinese hawthorn seed alcohol extract.
The preparation method is the same as that of Example 1 for Chinese hawthorn seed alcohol extract.
Test example
Laboratory apparatus and equipment
Key instrument:Anaerobic jar(Guangzhou Hitech Photoelectricity Biotech. Co., Ltd.), WGB-300 constant incubators (Chongqing Che Da laboratory apparatus factory), Millipore A10 ultra-pure-water treatment systems;SW-CJ-2F superclean benches(Suzhou is safe and sound Air technique Co., Ltd), the desk-top constant temperature supercentrifuges of Fresco(Heraeus), TU-181 type ultraviolet specrophotometers (Hewlett Packard, USA), -20 DEG C of refrigerators(Qingdao HaiEr Co., Ltd), -70 DEG C of refrigerators(Sanyo), LDZX- 4011 autoclave sterilizers(Shenan Medical Appliances Factory, Shanghai);0-250 DEG C of thermostatic drying chamber(Shanghai leap medical apparatus and instruments factory), AT261 electronic balances(Mettler), inverted light microscope(Olympus), ordinary optical microscope(Olympus), LABMED It is evacuated instrument.
Main equipment:Micropipettor(Gilson), oese, L-type glass rod, sterilize triangle spreading rod, alcolhol burner, tweezers (It needs to sterilize), tincture of iodine cotton balls, cotton ball soaked in alcohol, taper culture bottle, an aseptic sealed membrane, glass slide, cedar oil is various sterile Syringe, rack for test tube, 6 cm batch cultur wares, sterilize pipette tips(10,100,1000 μL), sterilize plastic test tube(1.5,5,10 mL), sterile emgloves.
Major experimental material and reagent
(1) strain:NCTC11637 plants of helicobacter pylori, escherichia coli(It is commonly called as Escherichia coli)Susceptibility type strain ATCC25922, staphylococcus aureus susceptibility type strain ATCC29213, ATCC14053 plants of candida albicans bacterium type strain are by Zhejiang The tight outstanding offer of river University Medical College Pathogen Biology system;
(2) mixed gas(85% nitrogen, 10% carbon dioxide, 5% oxygen):Pressure 10Mpa, Hangzhou the present work special gas are limited Company provides;
(3) helicobacter pylori selectivity Colombia blood plate(The Shanghai Mei Liai biological products Co., Ltd):The happy pellet in Hangzhou Bio tech ltd provides;
(4) brucella broth culture medium(Oxoid), brucella agar culture medium(Oxoid), husky fort agar medium (Barebio),Mueller-Hinton(MH)Agar medium(Barebio):Hangzhou Basebio Bio-tech Co., Ltd. provides.
(5) Grain stain liquid:Hangzhou Basebio Bio-tech Co., Ltd. provides.
(6) 6 mm blank drug susceptability test paper of diameter, tea polyphenols, sterile de- fiber Sheep Blood:Weigh the limited public affairs of biotechnology in Hangzhou pool Department provides;
(7) dimethyl sulfoxide (DMSO)(DMSO)(Sigma):The Hangzhou bio tech ltd Ze Heng provides;
(8) absolute ethyl alcohol, 75% ethyl alcohol, glycerine, cedar oil:Shanghai Sinopharm Chemical Reagent Co., Ltd. product.
Strain subject culture and dyeing microscopic examination
1. NCTC11637 plants of cultures of helicobacter pylori and dyeing microscopic examination
Use disinfection inoculation ring by NCTC11637 plants of intensive streak inoculations of helicobacter pylori in helicobacter pylorus in superclean bench On bacterium selectivity Colombia blood plate, tablet is inverted into anaerobic jar, and it is 650 mmHg to be pumped to negative pressure, is put into mixed Close gas(85% nitrogen, 10% carbon dioxide, 5% oxygen)It is 0 to air pressure, is so repeated 3 times, by 37 DEG C of culture 3 of anaerobic jar d。
On slide plus 1 drips physiological saline, is coated on physiological saline with disinfection inoculation ring picking lawn and is allowed to uniform, room temperature Middle natural drying forms mycoderm, crosses the 3-4 sterilization of alcohol lights and fixed mycoderm.Grain stain:Crystallization is added dropwise on slide mycoderm Purple dye liquor, room temperature dye 1 min, and the small water of tap water rinses, and iodine solution room temperature is added dropwise after drying and dyes 1 min, the small water punching of tap water It washes, destainer room temperature decoloration 15-30 Sec is added dropwise after drying(Slide need to slowly be rotated to improve decolorizing effect), the small water of tap water It rinses, is added dropwise after drying and redyes liquid chamber temperature 1 min of dyeing, the small water of tap water rinses, and arborvitae is added dropwise on slide mycoderm after drying Oil 1 drips, and slide room is on ordinary optical microscope objective table, in 10 × eyepiece, 100 × object microscopic observation.
2. ATCC25922 plants of cultures of escherichia coli and dyeing microscopic examination
ATCC25922 plants of intensive streak inoculations of escherichia coli are put down in MH agar with disinfection inoculation ring in superclean bench On plate, flat-plate inverted postpones 37 DEG C of culture 18-24 h.Dyeing and microscopy method are same as above.
3. ATCC29213 plants of cultures of Staphylococcus aureus and dyeing microscopic examination
Use disinfection inoculation ring by ATCC29213 plants of intensive streak inoculations of Staphylococcus aureus in MH agar in superclean bench On tablet, flat-plate inverted postpones 37 DEG C of culture 18-24 h.Dyeing and microscopy method are same as above.
4. the ATCC14053 plants of cultures of candida albicans bacterium and dyeing microscopic examination
Use disinfection inoculation ring by the ATCC14053 plants of intensive streak inoculations of candida albicans bacterium in Sha Baoqiong in superclean bench On fat tablet, flat-plate inverted postpones 37 DEG C of culture 2-3 d.Dyeing and microscopy method are same as above.
Test medicine and preparation
Test medicine stoste is prepared and appearance:Chinese hawthorn seed alcohol extract, 12.378 g extracts/100g crude drugs, is dissolved in 20 mL In DMSO, final concentration of 5000 mg/mL of crude drug, then with sterilizing distilled water be configured to respectively crude drug it is final concentration of 2500, 500,100,50,10 mg/mL solution, Chinese hawthorn seed alcohol extract are tasteless.
Anti-Heliobactorpylori drug sensitization test(Disk diffusion method)
1. prepared by drug sensitive test paper
6 mm blank susceptibility filter papers of diameter are put into culture dish, 121 DEG C of 30 min of autoclaving, 60 DEG C of drying It is spare.The extract drug stoste that the 20 above-mentioned various concentrations of μ L are drawn using sterilizing pipette tips is added in sterilizing filter paper on piece, makes filter paper Piece contained drug concentration is different.It is another to prepare DMSO and the sterilizing distilled water scraps of paper as a contrast.
2. prepared by tested bacterium solution
NCTC11637 plants of lawns of helicobacter pylori that fresh cultured is scraped with sterilizing L-type glass rod, are put into sterile saline In, helicobacter pylori is configured to 1 × 10 using turbidimetry7/ mL bacterium solutions(Growth is difficult, needs higher concentration bacterium solution).
3. drug sensitivity test operates
(1) the above-mentioned helicobacter pylori bacterium solutions of 200 μ L are drawn with sterilizing pipette tips add to sterilizing triangle painting in superclean bench On cloth stick, then it is spread evenly across on helicobacter pylori selectivity Colombia blood plate;
(2) with sterilizing tweezers, the scraps of paper containing concentration are equidistant is affixed on blood plate by different respectively, separately stick DMSO and sterilizing The distilled water scraps of paper are as a contrast.
(3) tablet is inverted into anaerobic jar, and it is 650 mmHg to be pumped to negative pressure, is put into mixed gas(85% nitrogen, 10% carbon dioxide, 5% oxygen)It is 0 to air pressure, is so repeated 3 times, by 37 DEG C of 3 d of culture of anaerobic jar.
(4) visually observed after cultivating around each scraps of paper whether there is or not antibacterial ring size, in DMSO and the sterilizing distilled water scraps of paper without antibacterial ring size Under the premise of, if antibacterial ring size occur in the drug containing scraps of paper, each inhibition zone diameter is measured as unit of mm.
4. experimental result:Chinese hawthorn seed alcohol extract anti-Heliobactorpylori drug sensitization test(Disk diffusion method)It the results are shown in Table 1.
Table 1
Drug sensitivity test(Plate dilution method)
1. prepared by tested bacterium solution
NCTC11637 plants of the helicobacter pylori of fresh cultured, escherichia coli ATCC25922 are scraped with sterilizing L-type glass rod ATCC14053 plants of strain, ATCC29213 plants of staphylococcus aureus, candida albicans bacterium lawns, are put into sterile saline, Helicobacter pylori is configured to 1 × 10 using turbidimetry7/ mL bacterium solutions(Growth is difficult, needs higher concentration bacterium solution), escherichia coli It is routinely configured to 1 × 10 with staphylococcus aureus and candida albicans bacterium6/ mL bacterium solutions.
2. prepared by drug containing tablet
(1) the Chinese hawthorn seed alcohol extract of 1 mL various concentrations is added in 6 cm diameter sterilizing plates;
(2) press product description and prepare brucella agar culture medium, MH agar mediums, husky fort agar medium, 121 DEG C of height 20 min of steam sterilizing, MH agar mediums, husky fort agar medium room temperature is pressed directly to use sterilized straw when being cooled to 55-60 DEG C 9 mL are taken to be added in above-mentioned dosing plate, gently turnery plate makes drug be uniformly mixed, and brucella agar culture medium room temperature is cooled to 10% de- fiber Sheep Blood of 37 DEG C of preheatings is first added at 55-60 DEG C, then presses in upper method addition dosing plate and gently turnery is flat Ware makes drug be uniformly mixed;
(3) above-mentioned plate is inverted 37 DEG C and is incubated 24 h inspection bacterium and drug is made to mix well.
3. drug sensitivity test operation and result judge
(1) above-mentioned one ring of bacterium solution of oese picking is used in superclean bench, and intensive streak inoculation is in above-mentioned drug containing tablet;
(2) it is inoculated with escherichia coli and staphylococcus aureus flat-plate inverted postpones 37 DEG C of 24 h of culture;Candida albicans bacterium (It is previous to claim Candida albicans, Bai Nian)Flat-plate inverted postpones 37 DEG C of 48 h of culture;It is inoculated with helicobacter pylori(Helicobacter pylori, HP)Tablet is inverted into anaerobic jar, and it is 650 mmHg to be pumped to negative pressure, is put into mixed gas(85% nitrogen, 10% carbon dioxide, 5% oxygen)It is 0 to air pressure, is so repeated 3 times, by 37 DEG C of 3 d of culture of anaerobic jar.
(3) each bacterium growing state, asepsis growth are visually observed after cultivating(-)Drug highest dilution is judged to the drug Minimum inhibitory concentration(Minimal inhibitory concentration, MIC), more bacterium, which grows, to be judged to(+), Shao Shuohuo The growth of only a few bacterium is judged to(±).
4. experimental result:Chinese hawthorn seed alcohol extracting medicament sensitivity test, dish dilution method the results are shown in Table 2, MIC judgement knots Fruit is shown in Table 3.
Table 2
Table 3

Claims (1)

1. the oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed, it is characterised in that
The oral care product includes Chinese hawthorn seed alcohol extract, the oral care product be toothpaste, mouthwash, chewing gum, candy, Effervescent tablet or mouth sprays, the chewing gum are grouped as by the group of following parts by weight:30-40 parts of xylitol, matrix 20-30 Part, 15-20 parts of sorbierite, 10-15 parts of maltitol liquor, 5-10 parts of D-mannital, 150-200 parts of citric acid, phosphatide 100-120 parts, 30-40 parts of Aspartame, 30-40 parts of essence, 1-5 parts of Chinese hawthorn seed alcohol extract;The toothpaste is by following weight The group of number is grouped as:80-100 parts of calcium monohydrogen phosphate, 20-30 parts of water, 5-10 parts of D-sorbite, 1-5 parts of hydrated silica gather - 8 1-5 parts of ethylene glycol, 1-5 parts of propylene glycol, 1-5 parts of laruyl alcohol sodium sulfovinate, 1-5 parts of cellulose gum, hydroxyethyl cellulose 1-5 parts, 1-5 parts of essence, 1-5 parts of sodium benzoate, 1-5 parts of saccharin sodium, 1-5 parts of Chinese hawthorn seed alcohol extract;The mouthwash by The group of following parts by weight is grouped as:90-100 parts of water, 10-20 parts of sorbierite, 5-10 parts of propylene glycol, xylitol 5-10 Part, 5-10 parts of PEG-400 polyethylene glycol, 1-5 parts of glycyrrhizic acid diformazan, 1-5 parts of sodium benzoate, 1-3 parts of essence, Chinese hawthorn seed alcohol 1-5 parts of extract;The candy is grouped as by the group of following parts by weight:50-100 parts of maltitol, 1-5 parts of citric acid are fragrant It is 1-5 parts smart, 1-5 parts of Chinese hawthorn seed alcohol extract;The effervescent tablet is grouped as by the group of following parts by weight:20-30 parts of VC, lemon 10-20 parts of lemon acid, 30-40 parts of sodium bicarbonate, 5-10 parts of sodium carboxymethyl starch, 10-20 parts of lactose, Aspartame 1-5 Part, 1-5 parts of essence, 1-5 parts of 1-5 parts of PVP-K30, PEG6000,1-5 parts of superfine silica gel powder, 1-5 parts of magnesium stearate, hawthorn 1-5 parts of core alcohol extract;The mouth sprays are grouped as by the group of following parts by weight:40-50 parts of water, 5-10 parts of sorbierite, 5-10 parts of propylene glycol, 1-5 parts of xylitol, 1-5 parts of PEG-400 polyethylene glycol, 1-5 parts of dipotassium glycyrrhizinate, sodium benzoate 1- 5 parts, 1-2 parts of essence, 1-5 parts of Chinese hawthorn seed alcohol extract;
The Chinese hawthorn seed alcohol extract is prepared using following methods:
1)Clean Chinese hawthorn seed after drying is taken, graininess is crushed to, 60 mesh sieve is crossed, obtains Chinese hawthorn seed powder;
2)By solid-liquid ratio(g/ml)1:10 ratio, in step 1)The ethyl alcohol of addition 70% in Chinese hawthorn seed powder, 80 DEG C of temperature is made Reflux extracting 4h, obtains extracting solution under the conditions of degree;
3)Extracting solution filter paper is filtered under diminished pressure, and is obtained a filtered solution, is added and step 2 in filter residue)70% ethyl alcohol of same volume, By step 2)Method extract filtering and removing slag after 2h again, obtain secondary filtered solution;
4)Merge a filtered solution and secondary filtered solution, with spin concentration under conditions of Rotary Evaporators 35r/min, 60 DEG C to cream Shape or powdery;
5)Yield is calculated, 4 DEG C of refrigerators save backup.
CN201510503560.3A 2015-08-17 2015-08-17 The oral care product with anti-helicobacter pylori of the alcohol extract containing Chinese hawthorn seed Active CN105012201B (en)

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CN103284233A (en) * 2013-06-01 2013-09-11 安徽山里仁食品股份有限公司 Preparation method of snowflake kernels
CN103536496A (en) * 2013-10-30 2014-01-29 昆明理工大学 Toothpaste and preparation method thereof
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CN1481897A (en) * 2002-09-10 2004-03-17 上海高科生物工程有限公司 Biotechnological formulation for suppressing and killing helicobacter pylori, its preparation and use
CN1502358A (en) * 2002-11-21 2004-06-09 毅 铁 Use of crataegus pit extract in preparation of medicine for treating gastropathy
CN102988525A (en) * 2012-12-13 2013-03-27 沈阳药科大学 Preparation method for total lignans in hawthorn seeds, and novel application
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