CN104997701A - Oral care product containing bletilla striata alcohol extract and having anti-Hp (helicobacter pylori) efficacy - Google Patents
Oral care product containing bletilla striata alcohol extract and having anti-Hp (helicobacter pylori) efficacy Download PDFInfo
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- CN104997701A CN104997701A CN201510503584.9A CN201510503584A CN104997701A CN 104997701 A CN104997701 A CN 104997701A CN 201510503584 A CN201510503584 A CN 201510503584A CN 104997701 A CN104997701 A CN 104997701A
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Abstract
The invention discloses an oral care product containing bletilla striata alcohol extract and having anti-Hp (helicobacter pylori) efficacy. The oral care product is characterized by containing the bletilla striata alcohol extract. The invention provides a kind of traditional Chinese medicine oral care products, a medicine extracting method is simple, dosage and ingredients are stable, and the products can go deep into an oral cavity directly. The oral care product cannot cause internal environment disturbance of the oral cavity when used for a long time, has no side effects, not only has similar functions including halitosis removal, oral ulcer treatment, resistance to common pathogenic bacteria in the oral cavity and inflammation diminishing to the conventional oral care products such as Chinese herbal medicine health care toothpaste and the like, but also has the functions of Hp killing and effective prevention of oral transmission of Hp.
Description
Technical field
The invention belongs to daily use chemicals product technical field, be specifically related to a kind of oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract.
Background technology
Helicobacter pylori, English name Helicobacter pylori, is called for short Hp.Helicobacter pylori is the pathogenic bacterium that a kind of infection rate, infectiousness are very strong.Australian scholar's Barry Marshall Barry J.Marshall in 1983 and Luo Bin Warren J.Robin Warren first in Gastritis Mucosa Biopsy tissue separation detection to helicobacter pylori.Within 1989, Australian scholar Krajden detects HP in the dental plaque of Gastritis Buccodental, and confirms that it is identical with gastric Hp from molecular level.Hp is the infectious disease that a kind of per os is propagated, and oral cavity Hp is the important depots of stomach Hp.The infringement of helicobacter pylori to health is great: be that to bring out the first kind of gastric cancer carcinogenic; The duodenal ulcer of 90% and the gastric ulcer of 80%, caused by helicobacter pylori infections.In China chronic active gastritis patient, the recall rate of helicobacter pylori is 95% to 100%; In duodenal ulcer patients, recall rate is 85% to 95%; In patients w ith peptic ulcer disease, recall rate is 70% to 80%; In patients with gastric cancer, recall rate is 80%.Epidemiological study China less than 12 years old teenager Hp infection rate is up to 40%, and adult Hp infection rate reaches more than 60%.Existing medical procedures adopts heavy dose of Multiple Classes of Antibiotics to take simultaneously and kills helicobacter pylori.The shortcoming of prior art is: 1. kill a large amount of probiotics, easily cause taking of the heavy dose of Multiple Classes of Antibiotics of disease recurrent exerbation 2., add the Drug resistance of helicobacter pylori, antibacterial produces drug resistance makes effective antibiotic therapeutic effect originally reduce or lose, and adds treatment difficulty and the medical treatment cost of patient disease.
As the saying goes, illness finds its way in by the mouth, and the infectious disease that direct oral cavity is propagated, except helicobacter pylori, also has escherichia coli, staphylococcus aureus and candida albicans bacterium etc., therefore, oral hygiene and important, and the nursing of oral hygiene, topmost is exactly the product using toothpaste, collutory and so on, existing oral care product, most interpolation disinfectant administers oral disease, but often use can increase the Drug resistance of oral cavity bacterium, destroys oral environment.
The Pseudobulbus Bletillae (Rhizoma Bletillae), (formal name used at school: Bletilla striata) has another name called Lian Jicao, Gan Gen, gives free of charge, indocalamus orchid, Zhu Lan, Rhizoma Bletillae, purple a species of orchid, hundred large bamboo hats with a conical crown and broad brim.Perennial herb bulbous plant (tuber), plant height 18-60 centimetre.Mainly be distributed in China, Japan and Upper Myanmar.The main florescence is in spring, but according to the difference of various places weather, evening, Winter Solstice, early summer all may be bloomed.The Pseudobulbus Bletillae (Rhizoma Bletillae) has medical value and Ornamental value widely.Be mainly used in astringing to arrest bleeding, detumescence and promoting granulation.Flower has purplish red, white, blue, yellow and powder isochrome, can potted plant indoor appreciation, also can intersperse in the Hua Tai comparatively covered, flower border or one jiao, garden.
Toothpaste be in daily life every day must use dental care products, along with the application of growth in the living standard collutory, chewing gum, confection, effervescent tablet or mouth sprays is also more and more extensive, the mouth care series products great majority such as current commercially available common health-care toothpaste use synthetic chemistry medicine to make antimicrobial component, and life-time service synthetic antibacterial agents exists potential harm and safety sex chromosome mosaicism to human body.Although more existing herbal health care toothpaste on market, antibacterial effect is also not ideal enough, does not particularly have and kills helicobacter pylori activity.
Summary of the invention
For problems of the prior art, the invention provides a kind of oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract.
The present invention is realized by the following technical programs:
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, is characterized in that this oral care product comprises Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, is characterized in that described oral care product is toothpaste, collutory, chewing gum, confection, effervescent tablet or mouth sprays.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, it is characterized in that described toothpaste is made up of the component of following parts by weight: calcium hydrogen phosphate 80-100 part, water 20-30 part, Sorbitol 5-10 part, hydrated silica 1-5 part, PEG-8 1-5 part, propylene glycol 1-5 part, sodium lauryl sulfate 1-5 part, cellulose gum 1-5 part, hydroxyethyl-cellulose 1-5 part, essence 1-5 part, sodium benzoate 1-5 part, saccharin sodium 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, it is characterized in that described collutory is made up of the component of following parts by weight: water 90-100 part, sorbitol 10-20 part, propylene glycol 5-10 part, xylitol 5-10 part, PEG-400 Polyethylene Glycol 5-10 part, glycyrrhizic acid diformazan 1-5 part, sodium benzoate 1-5 part, essence 1-3 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, it is characterized in that described chewing gum is made up of the component of following parts by weight: xylitol 30-40 part, gum base 20-30 part, sorbitol 15-20 part, maltitol liquor 10-15 part, D-mannital 5-10 part, citric acid 150-200 part, phosphatidase 1 00-120 part, aspartame 30-40 part, essence 30-40 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, is characterized in that described confection is made up of the component of following parts by weight: maltose alcohol 50-100 part, citric acid 1-5 part, essence 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, is characterized in that described effervescent tablet is made up of the component of following parts by weight: VC 20-30 part, citric acid 10-20 part, sodium bicarbonate 30-40 part, carboxymethyl starch sodium 5-10 part, lactose 10-20 part, aspartame 1-5 part, essence 1-5 part, PVP-K30 1-5 part, PEG6000 1-5 part, micropowder silica gel 1-5 part, magnesium stearate 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, it is characterized in that described mouth sprays is made up of the component of following parts by weight: water 40-50 part, sorbitol 5-10 part, propylene glycol 5-10 part, xylitol 1-5 part, PEG-400 Polyethylene Glycol 1-5 part, glycyrrhizic acid diformazan 1-5 part, sodium benzoate 1-5 part, essence 1-2 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
The described oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, is characterized in that described Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract adopts following methods preparation:
1) get the dry Pseudobulbus Bletillae (Rhizoma Bletillae) to pulverize, cross 60 mesh sieves, obtain common bletilla;
2) by solid-liquid ratio 1:7.5(g/mL) in common bletilla, add 95% ethanol, 95 DEG C of backflow extracting 1.5 h, obtain extracting solution;
3) extracting solution filter paper filtration under diminished pressure, obtains a filtered solution, adds 95% ethanol with step (2) same volume in filtering residue, by filter cleaner after upper method backflow extracting 1.5 h, obtains secondary filtered solution; Filtering residue always adds the ethanol of 95% of same volume, by filter cleaner after upper method extracting 1.5h, obtains three filtered solutions;
4) merge three filtered solutions, use Rotary Evaporators 35rpm, 60 DEG C of concentrated by rotary evaporations, to paste or drying, obtain Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract;
5) calculate yield, 4 DEG C of Refrigerator stores are for subsequent use.
The invention provides a quasi-tradition Chinese medicine oral care product, extract drugs method is simple, dosage ingredient stability, directly can go deep into oral cavity.Life-time service can not cause internal environment of oral cavity disorderly, has no side effect.The present invention not only has the similar antihalitosis of the mouth care series products such as existing herbal health care toothpaste, treating dental ulcer, anti-oral cavity common pathogen to reach antiinflammatory object, more kills helicobacter pylori, effectively stops the effect that helicobacter pylori per os is propagated.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in further detail, and provides detailed description of the invention.
Embodiment 1
Containing the toothpaste with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) alcohol extraction, be made up of the component of following parts by weight: calcium hydrogen phosphate 90 parts, 25 parts, water, Sorbitol 7 parts, hydrated silica 3 parts, PEG-8 3 parts, propylene glycol 4 parts, sodium lauryl sulfate 2 parts, cellulose gum 3 parts, hydroxyethyl-cellulose 3 parts, 3 parts, essence, sodium benzoate 3 parts, saccharin sodium 3 parts, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
Wherein, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract adopts following methods preparation:
1) get the dry Pseudobulbus Bletillae (Rhizoma Bletillae) to pulverize, cross 60 mesh sieves, obtain common bletilla;
2) by solid-liquid ratio 1:7.5(g/mL) in 200g common bletilla, add 95% ethanol 1500mL, 95 DEG C of backflow extracting 1.5 h, obtain extracting solution;
3) extracting solution filter paper filtration under diminished pressure, obtains a filtered solution, adds 95% ethanol of 1500mL in filtering residue, by filter cleaner after upper method backflow extracting 1.5 h, obtains secondary filtered solution; Filtering residue always adds the ethanol of 1500mL95%, by filter cleaner after upper method extracting 1.5h, obtains three filtered solutions;
4) merge three filtered solutions, use Rotary Evaporators 35rpm, 60 DEG C of concentrated by rotary evaporations, to paste or drying, obtain Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract;
5) calculate yield, 4 DEG C of Refrigerator stores are for subsequent use.
Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract extraction ratio=(Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract evaporate to dryness thing weight/Pseudobulbus Bletillae (Rhizoma Bletillae) coarse powder weight) × 100%.
Embodiment 2
Containing the collutory with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, be made up of the component of following parts by weight: 95 parts, water, sorbitol 15 parts, propylene glycol 7 parts, xylitol 8 parts, PEG-400 Polyethylene Glycol 8 parts, glycyrrhizic acid diformazan 3 parts, sodium benzoate 3 parts, 2 parts, essence, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
The preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract is with embodiment 1.
Embodiment 3
Containing the chewing gum with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, be made up of the component of following parts by weight: xylitol 35 parts, gum base 25 parts, sorbitol 17 parts, maltitol liquor 17 parts, D-mannital 8 parts, citric acid 170 parts, phosphatidase 1 10 parts, aspartame 35 parts, 35 parts, essence, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
The preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract is with embodiment 1.
Embodiment 4
Containing the confection with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) extract, be made up of the component of following parts by weight: maltose alcohol 75 parts, citric acid 3 parts, 3 parts, essence, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
The preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract is with embodiment 1.
Embodiment 5
Containing the effervescent tablet with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, be made up of the component of following parts by weight: VC25 part, citric acid 15 parts, sodium bicarbonate 35 parts, carboxymethyl starch sodium 7 parts, lactose 15 parts, aspartame 3 parts, 3 parts, essence, PVP-K30 3 parts, PEG6000 3 parts, micropowder silica gel 3 parts, magnesium stearate 3 parts, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
The preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract is with embodiment 1.
Embodiment 6
Containing the mouth sprays with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, be made up of the component of following parts by weight: 45 parts, water, sorbitol 8 parts, propylene glycol 7 parts, xylitol 3 parts, PEG-400 Polyethylene Glycol 3 parts, glycyrrhizic acid diformazan 2 parts, sodium benzoate 3 parts, 2 parts, essence, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 3 parts.
The preparation method of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract is with embodiment 1.
Test example
Experimental apparatus and equipment
Key instrument: anaerobic jar (Guangzhou Hitech Photoelectricity Biotech. Co., Ltd.), WGB-300 constant incubator (Chongqing Che Da experimental apparatus factory), Millipore A10 ultra-pure-water treatment system; SW-CJ-2F superclean bench (SuZhou Antai Air Tech Co., Ltd.), the desk-top constant temperature high speed centrifuge (Heraeus) of Fresco, TU-181 type ultraviolet spectrophotometer (Hewlett Packard, USA),-20 DEG C of refrigerators (Qingdao HaiEr Co., Ltd),-70 DEG C of refrigerators (Sanyo), LDZX-4011 autoclave sterilizer (Shenan Medical Appliances Factory, Shanghai); 0-250 DEG C of thermostatic drying chamber (Shanghai leap medical apparatus and instruments factory), AT261 electronic balance (Mettler), inverted light microscope (Olympus), ordinary optical microscope (Olympus), LABMED bleeds instrument.
Main equipment: micropipettor (Gilson), inoculating loop, L-type glass rod, sterilizing triangle spreading rod, alcohol burner, tweezers (needing sterilizing), iodine tincture cotton balls, cotton ball soaked in alcohol, taper culture bottle, an aseptic sealed membrane, microscope slide, cedar oil, various asepsis injector, test tube rack, 6 cm batch cultur wares, sterilizing rifle head (10,100,1000 μ L), sterilizing plastic test tube (1.5,5,10 mL), sterile milk Rubber gloves.
Major experimental material and reagent
(1) strain: helicobacter pylori NCTC11637 strain, escherichia coli (being commonly called as escherichia coli) susceptibility type strain ATCC25922, staphylococcus aureus susceptibility type strain ATCC29213, candida albicans bacterium type strain ATCC14053 strain are provided by the tight outstanding person of Pathogen Biology system of Medical College of Zhejiang Univ.;
(2) mist (85% nitrogen, 10% carbon dioxide, 5% oxygen): pressure 10Mpa, Hangzhou Jin Gong special gas company limited provides;
(3) helicobacter pylori selectivity Colombia blood plate (Mei Liai Shanghai biological product company limited): Yi Dan bio tech ltd, Hangzhou provides;
(4) brucella broth culture medium (Oxoid), brucella agar culture medium (Oxoid), husky fort agar culture medium (Barebio), Mueller-Hinton(MH) agar culture medium (Barebio): Hangzhou Basebio Bio-tech Co., Ltd. provides.
(5) Gram's staining liquid: Hangzhou Basebio Bio-tech Co., Ltd. provides.
(6) diameter 6 mm blank drug susceptability test paper, tea polyphenols, aseptic defiber Sheep Blood: Ze Heng bio tech ltd, Hangzhou provides;
(7) dimethyl sulfoxide (DMSO) (Sigma): Ze Heng bio tech ltd, Hangzhou provides;
(8) dehydrated alcohol, 75% ethanol, glycerol, cedar oil: Shanghai Chemical Reagent Co., Ltd., Sinopharm Group product.
Strain subject is cultivated and dyeing microscopic examination
1. helicobacter pylori NCTC11637 strain cultivation and dyeing microscopic examination
In superclean bench with disinfection inoculation ring by the intensive streak inoculation of helicobacter pylori NCTC11637 strain on helicobacter pylori selectivity Colombia blood plate, flat board is inverted in anaerobic jar, being pumped to negative pressure is 650 mmHg, putting into mist (85% nitrogen, 10% carbon dioxide, 5% oxygen) is 0 to air pressure, repetition like this 3 times, cultivates 3 d by anaerobic jar 37 DEG C.
Slide adds 1 normal saline, coats normal saline make it even with disinfection inoculation ring picking lawn, in room temperature, natural drying forms Mycoderma, crosses ethanol lights and sterilizes for 3-4 time and fix Mycoderma.Gram's staining: slide Mycoderma drips crystal violet dye liquor, room temperature dyes 1 min, the little water of tap water rinses, iodine liquid chamber temperature dyeing 1 min is dripped after drying, the little water of tap water rinses, drip destaining solution room temperature decolouring 15-30 Sec(after drying and need slow circumvolve slide to improve decolorizing effect), the little water of tap water rinses, drip after drying and redye liquid chamber temperature dyeing 1 min, the little water of tap water rinses, on slide Mycoderma, drip cedar oil 1 after drying, slide room on ordinary optical microscope object stage, at 10 × eyepiece, 100 × thing Microscopic observation.
2. escherichia coli ATCC25922 strain cultivation and dyeing microscopic examination
Use disinfection inoculation ring in superclean bench by the intensive streak inoculation of escherichia coli ATCC25922 strain on MH agar plate, 18-24 h is cultivated in flat-plate inverted postpone 37 DEG C.Dyeing and microscopy method the same.
3. Staphylococcus aureus ATCC29213 strain cultivation and dyeing microscopic examination
Use disinfection inoculation ring in superclean bench by the intensive streak inoculation of Staphylococcus aureus ATCC29213 strain on MH agar plate, 18-24 h is cultivated in flat-plate inverted postpone 37 DEG C.Dyeing and microscopy method the same.
4. candida albicans bacterium ATCC14053 strain cultivation and dyeing microscopic examination
Use disinfection inoculation ring in superclean bench by the intensive streak inoculation of candida albicans bacterium ATCC14053 strain on the agar plate of husky fort, 2-3 d is cultivated in flat-plate inverted postpone 37 DEG C.Dyeing and microscopy method the same.
Test medicine and preparation
The preparation of test medicine stock solution and outward appearance: Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, 8.760g extract/100g crude drug, be dissolved in 20 mL DMSO, its crude drug final concentration is 5000 mg/mL, then being mixed with crude drug final concentration respectively with sterilizing distilled water is 2500,500,100,50,10 mg/mL solution, and Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract mildly bitter flavor is puckery.
Anti-Heliobactorpylori drug sensitization test (disk diffusion method)
1. drug sensitive test paper preparation
Blank for diameter 6 mm susceptibility filter paper is put into culture dish, 121 DEG C of autoclaving 30 min, 60 DEG C of dry for standby.The extract medicine stock solution using sterilizing rifle head to draw the above-mentioned variable concentrations of 20 μ L is added on sterilizing filter paper, makes filter paper contained drug concentration different.Another preparation DMSO and the sterilizing distilled water scraps of paper are in contrast.
2. tested bacterium solution preparation
With the helicobacter pylori NCTC11637 strain lawn of sterilizing L-type glass rod scraping fresh cultured, put into sterile saline, adopt turbidimetry that helicobacter pylori is mixed with 1 × 10
7/ mL bacterium liquid (growth difficulty, needs higher concentration bacterium liquid).
3. drug sensitivity test operation
(1) in superclean bench, draw 200 μ L above-mentioned helicobacter pylori bacterium liquid with sterilizing rifle head to add on sterilizing triangle spreading rod, be then spread evenly across on helicobacter pylori selectivity Colombia blood plate;
(2) respectively difference is affixed on blood plate containing the concentration scraps of paper are equidistant with sterilizing tweezers, separately sticks DMSO and the sterilizing distilled water scraps of paper in contrast.
(3) flat board is inverted in anaerobic jar, and being pumped to negative pressure is 650 mmHg, and putting into mist (85% nitrogen, 10% carbon dioxide, 5% oxygen) is 0 to air pressure, so repeats 3 times, and anaerobic jar 37 DEG C is cultivated 3 d.
(4) after cultivating around each scraps of paper of perusal with or without bacterial restrain, at DMSO and the sterilizing distilled water scraps of paper without under the prerequisite of bacterial restrain, if bacterial restrain appears in the pastille scraps of paper, then in units of mm, measure each inhibition zone diameter.
4. experimental result: Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract anti-Heliobactorpylori drug sensitization test (disk diffusion method) the results are shown in Table 1.
Table 1
Drug sensitivity test (plate dilution method)
1. tested bacterium solution preparation
With the helicobacter pylori NCTC11637 strain of sterilizing L-type glass rod scraping fresh cultured, escherichia coli ATCC25922 strain, staphylococcus aureus ATCC29213 strain, candida albicans bacterium ATCC14053 strain lawn, put into sterile saline, adopt turbidimetry that helicobacter pylori is mixed with 1 × 10
7/ mL bacterium liquid (growth difficulty, needs higher concentration bacterium liquid), escherichia coli and staphylococcus aureus and candida albicans bacterium are all mixed with 1 × 10 routinely
6/ mL bacterium liquid.
2. pastille flat board preparation
(1) in 6 cm diameter sterilizing plates, add the Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract of 1 mL variable concentrations;
(2) press product description preparation brucella agar culture medium, MH agar culture medium, husky fort agar culture medium, 121 DEG C of high pressure steam sterilization 20 min, directly getting 9 mL with sterilizing suction pipe when MH agar culture medium, husky fort agar culture medium room temperature are cooled to 55-60 DEG C adds in above-mentioned dosing plate, turnery plate makes medicament mixed even gently, brucella agar culture medium room temperature first adds 10% defiber Sheep Blood of 37 DEG C of preheatings when being cooled to 55-60 DEG C, then to add in dosing plate by upper method and turnery plate makes medicament mixed even gently;
(3) above-mentioned plate is inverted 37 DEG C and is hatched 24 h and examine bacterium medicine is fully mixed.
3. drug sensitivity test operation and result judge
(1) in superclean bench, use inoculating loop picking above-mentioned bacterium liquid one ring, intensive streak inoculation is on above-mentioned pastille flat board;
(2) inoculate escherichia coli and staphylococcus aureus flat-plate inverted postpone 37 DEG C cultivation 24 h; 48 h are cultivated in candida albicans bacterium (claimed Candida albicans in the past, and read in vain) flat-plate inverted postpone 37 DEG C; Inoculation helicobacter pylori (
helicobacter pylori, HP) and flat board is inverted in anaerobic jar, and being pumped to negative pressure is 650 mmHg, and putting into mist (85% nitrogen, 10% carbon dioxide, 5% oxygen) is 0 to air pressure, so repeats 3 times, and anaerobic jar 37 DEG C is cultivated 3 d.
(3) cultivate and terminate each bacteria growing situation of rear perusal, the most high dilution of asepsis growth (-) medicine is judged to minimum inhibitory concentration (the minimal inhibitory concentration of this medicine, MIC), more bacteria growing is judged to (+), and minority or only a few bacteria growing are judged to (±).
4. experimental result: Pseudobulbus Bletillae (Rhizoma Bletillae) alcohol extraction medicament sensitivity test, dish dilution method, the results are shown in Table 2, MIC result of determination in table 3.
Table 2
Table 3
Claims (9)
1., containing the oral care product with anti-helicobacter pylori effect of Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract, it is characterized in that
This oral care product comprises Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract.
2. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 1, is characterized in that described oral care product is toothpaste, collutory, chewing gum, confection, effervescent tablet or mouth sprays.
3. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, it is characterized in that described toothpaste is made up of the component of following parts by weight: calcium hydrogen phosphate 80-100 part, water 20-30 part, Sorbitol 5-10 part, hydrated silica 1-5 part, PEG-8 1-5 part, propylene glycol 1-5 part, sodium lauryl sulfate 1-5 part, cellulose gum 1-5 part, hydroxyethyl-cellulose 1-5 part, essence 1-5 part, sodium benzoate 1-5 part, saccharin sodium 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
4. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, it is characterized in that described collutory is made up of the component of following parts by weight: water 90-100 part, sorbitol 10-20 part, propylene glycol 5-10 part, xylitol 5-10 part, PEG-400 Polyethylene Glycol 5-10 part, glycyrrhizic acid diformazan 1-5 part, sodium benzoate 1-5 part, essence 1-3 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
5. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, it is characterized in that described chewing gum is made up of the component of following parts by weight: xylitol 30-40 part, gum base 20-30 part, sorbitol 15-20 part, maltitol liquor 10-15 part, D-mannital 5-10 part, citric acid 150-200 part, phosphatidase 1 00-120 part, aspartame 30-40 part, essence 30-40 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
6. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, it is characterized in that described confection is made up of the component of following parts by weight: maltose alcohol 50-100 part, citric acid 1-5 part, essence 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
7. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, is characterized in that described effervescent tablet is made up of the component of following parts by weight: VC 20-30 part, citric acid 10-20 part, sodium bicarbonate 30-40 part, carboxymethyl starch sodium 5-10 part, lactose 10-20 part, aspartame 1-5 part, essence 1-5 part, PVP-K30 1-5 part, PEG6000 1-5 part, micropowder silica gel 1-5 part, magnesium stearate 1-5 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
8. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to claim 2, it is characterized in that described mouth sprays is made up of the component of following parts by weight: water 40-50 part, sorbitol 5-10 part, propylene glycol 5-10 part, xylitol 1-5 part, PEG-400 Polyethylene Glycol 1-5 part, glycyrrhizic acid diformazan 1-5 part, sodium benzoate 1-5 part, essence 1-2 part, Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract 1-5 part.
9. the oral care product with anti-helicobacter pylori effect containing Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract according to any one of claim 1-8, is characterized in that described Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract adopts following methods preparation:
1) get the dry Pseudobulbus Bletillae (Rhizoma Bletillae) to pulverize, cross 60 mesh sieves, obtain common bletilla;
2) by solid-liquid ratio 1:7.5(g/mL) in common bletilla, add 95% ethanol, 95 DEG C of backflow extracting 1.5 h, obtain extracting solution;
3) extracting solution filter paper filtration under diminished pressure, obtains a filtered solution, adds 95% ethanol with step (2) same volume in filtering residue, by filter cleaner after upper method backflow extracting 1.5 h, obtains secondary filtered solution; Filtering residue always adds the ethanol of 95% of same volume, by filter cleaner after upper method extracting 1.5h, obtains three filtered solutions;
4) merge three filtered solutions, use Rotary Evaporators 35rpm, 60 DEG C of concentrated by rotary evaporations, to paste or drying, obtain Pseudobulbus Bletillae (Rhizoma Bletillae) ethanol extract;
5) calculate yield, 4 DEG C of Refrigerator stores are for subsequent use.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105326656A (en) * | 2015-10-29 | 2016-02-17 | 山东省药学科学院 | Oral antibacterial composition containing nisin and polylysine and preparation method thereof |
| CN106943327A (en) * | 2017-03-31 | 2017-07-14 | 镇远县道益堂生物科技有限公司 | A kind of bletilla striata toothpaste and preparation method thereof |
| CN107721927A (en) * | 2017-11-03 | 2018-02-23 | 隆安县荣胜养殖有限公司 | A kind of method of the extraction purification bletilla striata alcohol from the bletilla striata |
| CN112438940A (en) * | 2020-12-17 | 2021-03-05 | 宁波市朱氏贸易有限公司 | Toothpaste capable of effectively inhibiting oral helicobacter pylori |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178844A (en) * | 2011-04-28 | 2011-09-14 | 孔建 | Oral Chinese medicine preparation for treating gum swelling and pain and preparation method thereof |
| CN103520052A (en) * | 2013-10-18 | 2014-01-22 | 柳州两面针股份有限公司 | Application of hyacinth bletilla extract in preparing oral care product |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102178844A (en) * | 2011-04-28 | 2011-09-14 | 孔建 | Oral Chinese medicine preparation for treating gum swelling and pain and preparation method thereof |
| CN103520052A (en) * | 2013-10-18 | 2014-01-22 | 柳州两面针股份有限公司 | Application of hyacinth bletilla extract in preparing oral care product |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105326656A (en) * | 2015-10-29 | 2016-02-17 | 山东省药学科学院 | Oral antibacterial composition containing nisin and polylysine and preparation method thereof |
| CN106943327A (en) * | 2017-03-31 | 2017-07-14 | 镇远县道益堂生物科技有限公司 | A kind of bletilla striata toothpaste and preparation method thereof |
| CN107721927A (en) * | 2017-11-03 | 2018-02-23 | 隆安县荣胜养殖有限公司 | A kind of method of the extraction purification bletilla striata alcohol from the bletilla striata |
| CN112438940A (en) * | 2020-12-17 | 2021-03-05 | 宁波市朱氏贸易有限公司 | Toothpaste capable of effectively inhibiting oral helicobacter pylori |
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Application publication date: 20151028 |