WO2019167458A1 - 汚染防止剤及び皮膚外用剤 - Google Patents
汚染防止剤及び皮膚外用剤 Download PDFInfo
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- WO2019167458A1 WO2019167458A1 PCT/JP2019/001209 JP2019001209W WO2019167458A1 WO 2019167458 A1 WO2019167458 A1 WO 2019167458A1 JP 2019001209 W JP2019001209 W JP 2019001209W WO 2019167458 A1 WO2019167458 A1 WO 2019167458A1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
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- the present invention relates to a contamination inhibitor characterized by containing mannosyl erythritol lipid (MEL). More specifically, the present invention relates to a contamination preventive agent useful for preventing adhesion of air pollutants such as microparticulate materials containing MEL as an active ingredient, and a skin external preparation containing the contamination preventive agent.
- MEL mannosyl erythritol lipid
- External stimuli are broadly divided into physical stimuli such as drying, sunlight, and temperature, and chemical stimuli caused by atmospheric aerosol particles that are liquid and solid fine particles floating in the environment.
- physical stimuli such as drying, sunlight, and temperature
- chemical stimuli caused by atmospheric aerosol particles that are liquid and solid fine particles floating in the environment.
- Representative examples of atmospheric aerosol particles include various chemical substances, xenobiotics, particles, etc., automobiles, thermal power plants, incinerators, factories, etc.
- microparticulate matter so-called PM2.5
- PM2.5 microparticulate matter
- the adverse effects on the living body caused by the adhesion of these microparticulate substances to the skin and hair are being widely recognized, and this has attracted attention in conjunction with the recent increase in health consciousness.
- the adverse effect on the skin caused by microparticulates is that microparticulates penetrate deep into the stratum corneum, stimulating the immune system as xenobiotics, and inflammatory mediators such as prostaglandin E2 (PGE2) and histamine. Mechanisms that promote production and induce inflammation have been proposed.
- PGE2 prostaglandin E2
- Mechanisms that promote production and induce inflammation have been proposed.
- substances that can cause damage do not adhere to the skin and / or the adverse effects caused by the adhering substances causing irritation are reduced (antioxidation or neutralization). It is done.
- Patent Document 1 As an approach that does not allow air pollutants to adhere to the skin, anti-poration containing hyaluronic acid and / or a salt thereof as an active ingredient as an anti-poration agent and anti-poration skin external composition for reducing skin damage caused by air pollutants An agent has been proposed (Patent Document 1).
- Patent Document 2 As an approach to alleviate the adverse effects of irritation-causing substances adhering to the skin, the extract of the genus Hippophae genus Gumiaceae suppresses the increase in PGE2 caused by atmospheric aerosol particles such as particulate air pollutants, thereby reducing the atmospheric aerosol An action of suppressing skin inflammation caused by particles has been found (Patent Document 2).
- a protective effect against a microparticulate substance by a polymer compound contained in a living body and an effect of suppressing a biological reaction caused by a microparticulate substance caused by an organic acid containing a plant extract or a metal complex have been proposed.
- the proposal of a new material that exerts an effect with different mechanisms against the influence of contaminants on the skin is expected to have a synergistic effect of preventing contamination of microparticulate substances, and formulation formulation formulation From the viewpoint of diversification of the above, and from the viewpoint of providing a safer component having the effect of protecting the fine particulate matter, it is required to propose a new material having an effect of preventing contamination of the fine particulate matter.
- biosurfactant that is an amphiphilic substance having both a hydrophilic group and a lipophilic group and has a surface-active ability.
- Biosurfactants which are microorganism-derived surfactants, are being studied as environmentally advanced surfactants that are highly safe and have a low environmental impact and excellent biodegradability.
- biosurfactants can be classified into five types: sugar type, acyl peptide type, phospholipid type, fatty acid type and polymer compound type. Among them, sugar type biosurfactants are the most studied, and bacteria And many types of substances produced by yeast have been reported.
- MEL mannosyl erythritol lipid
- JP 2017-186276 A Japanese Unexamined Patent Publication No. 2016-216366 JP 2016-88929 A
- An object of the present invention is to provide a highly safe antifouling effect agent having an excellent antifouling effect, and a skin external preparation using the antifouling agent.
- MEL mannosyl erythritol lipid
- Item 1 An antifouling agent comprising mannosyl erythritol lipid (MEL). Item 2. Item 2. The antifouling agent according to Item 1, wherein the mannosyl erythritol lipid (MEL) is MEL-B. Item 3. A structure in which MEL-B is represented by the general formula (2) or the general formula (3) (in the general formula (2) and the general formula (3), the substituent R1 may be the same or different and has 4 to 24 carbon atoms. Item 3. An antifouling agent according to Item 1 or 2, which has an aliphatic acyl group of Item 4. Item 4. The pollution inhibitor according to Item 3, wherein R1 has 8 to 14 carbon atoms. Item 5. Item 5.
- the contamination inhibitor according to any one of Items 1 to 4 which is a composition obtained by dispersing and dissolving mannosyl erythritol lipid (MEL) in a solvent.
- Item 6. A topical skin preparation comprising the contamination inhibitor according to any one of Items 1 to 5.
- Item 7. The skin external preparation according to Item 6, containing 0.001 to 10.0% by mass of mannosyl erythritol lipid (MEL).
- Item 8. Item 6. A method for suppressing adhesion of atmospheric aerosol particles to skin or hair by using the antifouling agent according to any one of Items 1 to 5.
- the present invention it is possible to provide a contamination inhibitor containing MEL as an active ingredient.
- the present invention uses the self-organizing ability of MEL and forms a contamination prevention layer made of MEL on the surface of the material to be coated, thereby preventing the adsorption and penetration of atmospheric aerosol particles into the material to be coated. Since it can be expected to reduce skin damage, it is effective as an antifouling agent.
- Example 2 it is a figure which shows the result of having performed the pollution prevention evaluation using the bio skin by containing MEL.
- Example 2 it is a figure which shows the removal rate of a city air dust as a city air dust pollution prevention effect to the bio skin by including MEL.
- the present invention is described in detail below.
- the antifouling agent of the present invention is characterized by using MEL as an active ingredient.
- an air pollutant that is a main pollution target refers to a harmful substance or a harmful substance group existing in the atmosphere.
- Specific examples include smoke, dust, automobile exhaust, specific substances, hazardous air pollutants, and volatile organic compounds (VOC) that are regulated by the Air Pollution Control Act (independent administrative agency environmental regeneration) Conservation Organization Homepage; https://www.erca.go.jp/yobou/taiki/taisaku/01_01.html) is not limited to these. More specifically, there are suspended particulate substances composed of a plurality of types of harmful substances such as PM10 and PM2.5.
- contamination prevention is to prevent atmospheric aerosol particles such as fine particulate matter from adhering to the skin and hair. Furthermore, by preventing atmospheric aerosol particles such as microparticulate matter from adhering to the skin and hair, skin damage caused by them is prevented and skin damage is alleviated.
- Atmospheric aerosol particles are typical as external stimuli for the skin and hair, and include various chemical substances, xenobiotics, particles, etc., smoke from automobiles, thermal power plants, incinerators, factories, etc. Volcanic eruptions, particulate matter such as dust and yellow sand derived from soil particles, etc., exhaust gases such as carbon monoxide, sulfur oxides, nitrogen oxides derived from combustion, etc., hydrocarbons and nitrogen oxides, etc. Derived from photochemical oxidants such as ozone and polycyclic aromatic hydrocarbons generated by photochemical reaction, exhaust gases such as volatile organic compounds derived from combustion and volatilization from petroleum products, fine particles, minerals, industrial products, etc. And fine particles such as asbestos and cigarette smoke. Among them, urban air dust containing a lot of fine particulate matter is widely recognized as having adverse effects on living bodies, particularly in the Asian region, from the viewpoint of being transported across borders from neighboring countries.
- the antifouling agent provided by the present invention is excellent in the effect of preventing the adhesion of atmospheric aerosol particles such as fine particulate matter, and therefore can suppress skin damage caused by atmospheric aerosol particles such as fine particulate matter. Furthermore, since MEL itself has an anti-inflammatory effect and a barrier function improving effect, it can be expected to suppress and improve inflammation and rough skin caused by atmospheric aerosol particles such as fine particulate matter. Moreover, the improvement of the effect is anticipated by adding a various compounding component.
- This antifouling agent can be provided as cosmetics, quasi-drugs, medical supplies, hygiene products, and pharmaceuticals.
- MEL The structure of MEL is shown in general formula (1).
- the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different.
- MEL is classified into four types, MEL-A, MEL-B, MEL-C, and MEL-D, based on the presence or absence of acetyl groups at the 4th and 6th positions of mannose.
- MEL may be used independently, 2 or more types of MEL can also be used together.
- the substituents R2 and R3 are both acetyl groups.
- the substituent R2 is an acetyl group, and the substituent R3 is a hydrogen atom.
- the substituent R2 is a hydrogen atom, and the substituent R3 is an acetyl group.
- the substituents R2 and R3 are both hydrogen atoms.
- the carbon number of the substituent R1 in the MEL represented by the above general formula (1) is the carbon number of the fatty acid constituting the triglyceride, which is an oil / fat contained in the MEL production medium, and the utilization of the fatty acid of the MEL-producing bacterium to be used. Varies depending on the degree.
- an unsaturated fatty acid residue can be included as the substituent R1 unless the MEL-producing bacterium assimilates up to the double bond portion of the unsaturated fatty acid. .
- the obtained MEL is usually in the form of a mixture of compounds having different fatty acid residue portions of the substituent R1.
- the MEL preferably used for the antifouling agent of the present invention is MEL-A, MEL-B or MEL-C, more preferably MEL- having a structure represented by the general formula (2) or the general formula (3).
- B. More preferred is MEL-B having a structure represented by the general formula (3).
- the substituent R1 is an aliphatic acyl group having 4 to 24 carbon atoms which may be the same or different.
- the number of carbon atoms of the substituent R1 is not particularly limited as long as it is within the above range, but it is more preferably 8 to 14.
- the substituent R1 may be a saturated aliphatic acyl group or an unsaturated aliphatic acyl group, and is not particularly limited. When it has an unsaturated bond, you may have a some double bond, for example.
- the carbon chain may be linear or branched. In the case of an oxygen atom-containing hydrocarbon group, the number and position of oxygen atoms contained are not particularly limited.
- MEL can be cultured and produced using microorganisms.
- Pseudozyma sp. MEL mixture obtained by cultivating etc. can be purified using silica gel column chromatography to isolate each of MEL-A, MEL-B and MEL-C.
- MEL The usage form of the MEL added to the composition of the present invention is arbitrary.
- MEL an extract from a microorganism culture solution or a purified high-purity product is used. Alternatively, it may be used after being suspended in water or dissolved in a solvent.
- Suitable MEL-B is a purified high-purity product, and a product purified to a purity of 80% to 100%, more preferably 90% to 100% is preferably used.
- the antifouling agent of the present invention refers to MEL itself or a composition formed by dispersing and dissolving MEL in a solvent.
- the solvent is not particularly limited as long as it can disperse and dissolve MEL, and may be either a water-soluble base or an oil-soluble base.
- the water-soluble base include water, ethanol, isopropanol, ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, polypropylene glycol, 1,3-butylene glycol, 1,3-propanediol, and pentylene glycol. Glycerin and the like.
- oil-soluble base examples include avocado oil, olive oil, orange oil, chamomile oil, kukui nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, castor oil, cottonseed oil, peanut oil, turtle oil Oil, mink oil, egg yolk oil, palm oil, palm kernel oil, mole, palm oil, hydrogenated oil, hydrogenated castor oil, waxes, lanolins, liquid paraffin, petrolatum, squalane, lauric acid, myristic acid, palmitic acid, stearin Natural fatty acids such as acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, Isopentanoic acid, lauryl alcohol, cetanol
- the blending amount of MEL in the antifouling agent is not particularly limited as long as it is within a range that can impart the antifouling effect to the external preparation for skin, but is usually preferably 0.001% by mass to 100.0% by mass, and preferably 0.005% by mass Is more preferably from 95% by weight, and further preferably from 0.01% to 90% by weight.
- the present invention provides a stain-preventing agent containing mannosyl erythritol lipid and a skin external preparation containing the same, but the amount of MEL may be added within a range that exhibits a stain-preventing effect.
- the external preparation for skin it is usually preferably 0.001 to 10.0% by mass, more preferably 0.01 to 1.0% by mass, and further preferably 0.05 to 0.5% by mass. If the amount is less than 0.001% by mass, the MEL layer structure may not be sufficiently formed on the surface of the material to be coated, and the antifouling effect may not be sufficiently exhibited. On the other hand, if the amount exceeds 10.0% by mass, There is concern about the decline in workability.
- components other than the MEL that can be contained in the external preparation for skin are not particularly limited as long as the effects of the present invention are not impaired, and can be appropriately selected according to the purpose. .
- the antifouling agent of the present invention has an excellent anti-adhesion effect on atmospheric aerosol particles such as microparticulate substances and is excellent in safety, and is therefore suitable for use as a skin external preparation.
- the external preparation for skin of the present invention contains the above-described anti-staining agent of the present invention, and further contains other components as necessary.
- the topical skin preparation means various drugs applied to the skin, and the classification is not particularly limited, and includes, for example, a wide range of skin cosmetics, quasi drugs, pharmaceuticals, and the like. .
- the form of the external preparation for skin is not particularly limited and may be appropriately selected depending on the intended purpose.
- ointment cream, milky lotion, serum, lotion, pack, foundation, lip balm, bath preparation, hair artic , Hair lotion, soap, body shampoo, etc.
- the other components are not particularly limited and may be appropriately selected depending on the purpose.
- Components usually used for producing a skin external preparation such as an oily component, a surfactant, a solvent, a moisturizer, an increase agent, and the like.
- Adhesives, medicinal ingredients, preservatives, pigments, powders, pH adjusters, ultraviolet absorbers, antioxidants, fragrances, and the like can be appropriately blended.
- oily components examples include avocado oil, olive oil, orange oil, chamomile oil, cucumber nut oil, safflower oil, soybean oil, camellia oil, corn oil, rapeseed oil, castor oil, cottonseed oil, peanut oil, turtle oil, mink oil , Egg yolk oil, palm oil, palm kernel oil, molasses, coconut oil, hardened oil, hardened castor oil, waxes, lanolins, liquid paraffin, petrolatum, squalane, lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid Natural fatty acids such as oleic acid, 12-hydroxystearic acid, undecylenic acid, lanolin fatty acid, isononanoic acid, caproic acid, 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2-ethylhexanoic acid, isopentanoic acid, lauryl Alcohol, cetanol, steary
- anionic surfactant (carboxylate, sulfonate, sulfate ester salt, phosphate ester salt), cationic surfactant (amine salt, quaternary ammonium salt), amphoteric surfactant ( Carboxylic acid type amphoteric surfactant, sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant), nonionic surfactant (ether type nonionic surfactant, ether ester) Type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing type nonionic surfactant), other surfactants (natural surfactants, protein hydrolysates) Derivatives, polymer surfactants, surfactants containing titanium and silicon, and fluorocarbon surfactants).
- solvent examples include ethanol, isopropanol, ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, polypropylene glycol, 1,3-butylene glycol, 1,3-propanediol, pentylene glycol, glycerin and the like. It is done.
- any component that is generally used in cosmetics may be used, and examples thereof include pyrrolidone carboxylic acid, lactic acid, sodium lactate, amino acid, sodium hyaluronate, and chondroitin sulfate. It is done. By adding these moisturizing agents, effects such as imparting moisturizing properties to the external skin preparation for preventing contamination of the present invention are expected.
- any of ingredients generally used in cosmetics may be used, such as guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, xanthan gum, Curdlan, methyl cellulose, hydroxyethyl cellulose, carboxymethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol, Examples include polyvinyl pyrrolidone, carboxyvinyl polymer, alkyl-modified carboxyvinyl polymer, sodium polyacrylate, bentonite and the like. These thickeners are expected to have effects such as improved stability and improved feeling of use with respect to the skin external preparation for preventing contamination of the present invention.
- any of ingredients generally used in cosmetics may be used.
- Addition of these medicinal ingredients is expected to impart an antioxidant action and an anti-aging effect to the external skin preparation for preventing contamination of the present invention.
- any component that is generally used in cosmetics may be used, for example, parabens such as methylparaben, ethylparaben, propylparaben, butylparaben, phenoxyethanol, ethylhexylglycerin and the like. Can be mentioned.
- parabens such as methylparaben, ethylparaben, propylparaben, butylparaben, phenoxyethanol, ethylhexylglycerin and the like.
- any component that is generally used in cosmetics may be used.
- citric acid, Na citrate, lactic acid, Na lactate, Na hydroxide, K hydroxide, etc. Is mentioned.
- the antifouling agent and the external preparation for skin of the present invention can be used on a daily basis, and the adhesion of atmospheric aerosol particles such as fine particulate matter to the skin is prevented by the action of the MEL which is an active ingredient. Is. Therefore, the antifouling agent and the external preparation for skin of the present invention can suppress the occurrence of skin damage caused by atmospheric aerosol particles such as microparticulate substances, and the anti-inflammatory effect of MEL itself can reduce the effects of microparticulate substances and the like. From the viewpoint of suppressing inflammation caused by atmospheric aerosol particles, it can be suitably used as an external preparation for skin used for protecting the skin from the external environment.
- MEL has an effect of improving the barrier function, so it can be expected to show useful effects on the skin by different mechanisms.
- it becomes possible to effectively prevent and improve various skin symptoms such as dry skin, rough skin, atopic dermatitis, psoriasis caused by inflammation caused by fine particulate matter. Be expected.
- the antifouling agent and external preparation for skin of the present invention are preferably applied to humans, but as long as the effects are exerted, animals other than humans (for example, mice, rats, hamsters, dogs, It is also possible to apply to cats, cows, pigs, monkeys, etc.).
- the antifouling agent of the present invention contains natural MEL derived from microbial fermentation as an active ingredient, and is advantageous in terms of excellent safety.
- Example 1 Production of MEL-B Pseudozyma tsukubaensis (NBRC 1940) was cultured with aeration and agitation for 48 hours in a YM medium at a culture temperature of 26 ° C. using a 500 ml Sakaguchi flask. The obtained solution is used as a seed culture solution. The obtained seed culture solution was cultured with aeration and agitation in a YM medium (containing 5% olive oil) at a culture temperature of 26 ° C. for 7 days using a 10 L jar fermenter. An equal amount of ethyl acetate was added to the culture and stirred for distribution.
- a YM medium containing 5% olive oil
- Example 2 MEL-B urban dust prevention test (sample)
- the test sample was prepared by the preparation method described below with the composition shown in Table 1.
- the sample used in the present invention was MEL-B obtained in Example 1 described above.
- a in Table 1 was heated to 70 ° C., and B was added and stirred and dissolved.
- a C + D phase (D phase in the case of the comparative example) dissolved in advance by heating at 60 ° C. was gradually added to prepare a test sample in which MEL was dispersed.
- 10 mg of urban air dust (NIES CRM No. 28) was weighed into a plastic bag (manufactured by Production Japan Co., Ltd.), covered and shaken with a plastic bag to create a simulated atmosphere in which urban air dust was scattered.
- the bioskin coated with the test sample was placed in a plastic bag in which city air dust was scattered and shaken to attach the city air dust to the bioskin.
- a plurality of bioskins with urban atmospheric dust adhered were photographed with a microscope (manufactured by OMRON) and the state immediately after adhesion was recorded. Thereafter, the bioskin with urban air dust adhered was exposed to 200 g of shower-like running water to remove the surface moisture so as not to be rubbed. A part similar to the part photographed immediately after the attachment was photographed again with a microscope, and the state after dropping of urban atmospheric dust by running water was recorded (see FIG. 1).
- Photographs taken before and after running water treatment are converted to black and white using image processing software ImageJ (https://imagej.nih.gov/ij/), and the area value of the black part is digitized as urban air dust adhesion area, and running water treatment The urban air dust removal rate before and after was calculated. The study was double blinded.
- Example 3 Below, the formulation example as a skin external preparation in this invention is shown.
- Lotion Toner lotion was produced by a conventional method according to the following composition. ⁇ Purified water ... 90.70g ⁇ Glycerin ... 3.00g ⁇ Phenoxyethanol ... 0.20g ⁇ Butylene glycol ⁇ ⁇ ⁇ 5.00g ⁇ Pentylene glycol ⁇ ⁇ ⁇ 1.00g ⁇ MEL-B ... 0.10g
- Cream According to the following composition, a cream was produced by a conventional method.
- ⁇ Purified water 58.50 g ⁇ Butylene glycol ⁇ ⁇ ⁇ 10.00g ⁇ Glycerin ... 5.00g ⁇ Phenoxyethanol ... 0.20g ⁇ Ethylhexylglycerin ⁇ ⁇ ⁇ 0.20g ⁇ Squalane ... 10.00g ⁇ Olive oil ... 10.00g ⁇ Behenyl alcohol ... 2.50 g ⁇ Polyglyceryl pentastearate-10 ... 1.90 g ⁇ Stearoyl lactate Na ... 0.60g ⁇ Cetyl palmitate ... 1.00 g ⁇ MEL-B ... 0.10g
- the anti-staining agent and the external preparation for skin of the present invention prevent air aerosol particles such as fine particulate matter from adhering to the skin and hair, thereby preventing damage such as inflammation to the skin due to air pollutants. Can be expected. Furthermore, it can be suitably used for an external preparation for skin from the viewpoint that synergistic skin damage can be suppressed by the anti-inflammatory effect and barrier function improving effect of MEL.
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Abstract
Description
一方、皮膚に付着した刺激原因物質による悪影響を緩和するアプローチとしては、グミ科ヒッポファエ属の抽出物が粒子状大気汚染物質等の大気エアロゾル粒子に起因するPGE2の上昇を抑制することで、大気エアロゾル粒子に起因する皮膚炎症を抑制する作用を見出している(特許文献2)。また、皮膚内部に浸透した汚染物質が表皮細胞で皮膚炎症を惹起するメカニズムに注目し、アミノ酸類の亜鉛塩及び/又は亜鉛錯体による細胞傷害緩和作用、CYP1過剰誘導作用、COX-2過剰誘導作用、IL-8過剰誘導作用の抑制といった顕著な有用性を見出している(特許文献3)。
項2.マンノシルエリスリトールリピッド(MEL)がMEL-Bである項1に記載の汚染防止剤。
項3.MEL-Bが一般式(2)または一般式(3)にて示される構造(一般式(2)及び一般式(3)中、置換基R1は同一でも異なっていてもよい炭素数4~24の脂肪族アシル基を示す)を有する項1または2に記載の汚染防止剤。
項5.マンノシルエリスリトールリピッド(MEL)が溶媒中に分散・溶解して成る組成物である項1から4のいずれかに記載の汚染防止剤。
項6.項1から5のいずれかに記載の汚染防止剤を含む皮膚外用剤。
項7.マンノシルエリスリトールリピッド(MEL)を0.001~10.0質量%含有する項6に記載の皮膚外用剤。
項8.項1から5のいずれかに記載の汚染防止剤を用いることにより大気エアロゾル粒子の皮膚または毛髪への付着を抑制する方法。
本発明の汚染防止剤は、MELを有効成分とすることを特徴とする。
MELの構造を一般式(1)に示す。一般式(1)中、置換基R1は、同一でも異なっていてもよい炭素数4~24の脂肪族アシル基である。MELは、マンノースの4位及び6位のアセチル基の有無に基づいて、MEL-A、MEL-B、MEL-C及びMEL-Dの4種類に分類される。MELは、単独で使用してもよいが、2種以上のMELを併用することもできる。
Pseudozyma tsukubaensis(NBRC 1940)を、500ml容坂口フラスコを用いて、YM培地にて培養温度26℃で48時間通気攪拌培養した。得られた溶液を種培養液とする。得られた種培養液を、10L容ジャーファーメンターを用いて、YM培地(5% オリーブ油を含む)にて、培養温度26℃で7日間通気攪拌培養した。培養液に等量の酢酸エチルを加え攪拌し分配を行った。酢酸エチル層に無水硫酸Naを適量加え30分間静置させた後、加温濃縮し、粗MEL-Bを得た。得られた粗MEL-Bを、シリカゲルカラムを用いて、クロロホルム:アセトン=1:0、クロロホルム:アセトン=9:1、クロロホルム:アセトン1:1、クロロホルム:アセトン=3:7、クロロホルム:アセトン=0:1で溶出した。MEL-B画分を分取・濃縮し、精製MEL-Bを得た。
(試料)
被験試料としては、表1に示す組成で、以下に記載する調製方法により調製した。なお、本発明に用いた試料は、上述の実施例1で得られたMEL-Bを用いた。
はじめに表1におけるAを70℃まで加温し、Bを添加して撹拌・溶解した。続いて、A+B相を撹拌しながら、あらかじめ60℃で加温溶解したC+D相(比較例の場合にはD相)を徐々に添加し、MELを分散させた被験試料を調製した。
人工皮膚模型バイオスキン(ビューラックス製)に対して、被験試料を2mg/cm2となるように均一に塗布し、静置して乾燥させた(n=3)。都市大気粉塵(NIES CRM No.28)10mgをポリ袋(生産日本社製)に秤り取り、蓋をしてポリ袋を振り、都市大気粉塵が飛散する疑似大気を作製した。都市大気粉塵を飛散させたポリ袋に被験試料を塗布したバイオスキンを入れて振ることで、バイオスキンに都市大気粉塵を付着させた。都市大気粉塵を付着させたバイオスキンをマイクロスコープ(オムロン製)で複数点撮影し、付着直後の状態を記録した。その後、都市大気粉塵が付着したバイオスキンをシャワー状の流水200gに晒し、表面の水分をこすらないように除いた。付着直後に撮影した箇所と同様の箇所を再度マイクロスコープで撮影し、流水による都市大気粉塵の脱落後の状態を記録した(図1参照)。流水処理前後の撮影写真について、画像処理ソフトウェアImageJ(https://imagej.nih.gov/ij/)を用いて白黒変換し、黒色部分の面積値を都市大気粉塵付着面積として数値化し、流水処理前後の都市大気粉塵除去率を算出した。試験は二重盲検化して実施した。
以下に、本発明における皮膚外用剤としての処方例を示す。
下記組成に従い、化粧水を常法により製造した。
・精製水・・・90.70g
・グリセリン・・・3.00g
・フェノキシエタノール・・・0.20g
・ブチレングリコール・・・5.00g
・ペンチレングリコール・・・1.00g
・MEL-B・・・0.10g
下記組成に従い、ジェルを常法により製造した。
・精製水・・・88.80g
・カルボマー・・・0.30g
・キサンタンガム・・・0.10g
・アルギニン・・・0.40g
・グリセリン・・・5.00g
・フェノキシエタノール・・・0.20g
・ブチレングリコール・・・5.00g
・MEL-B・・・0.2g
下記組成に従い、クリームを常法により製造した。
・精製水・・・58.50g
・ブチレングリコール・・・10.00g
・グリセリン・・・5.00g
・フェノキシエタノール・・・0.20g
・エチルヘキシルグリセリン・・・0.20g
・スクワラン・・・10.00g
・オリーブ油・・・10.00g
・ベヘニルアルコール・・・2.50g
・ペンタステアリン酸ポリグリセリル-10・・・1.90g
・ステアロイル乳酸Na・・・0.60g
・パルミチン酸セチル・・・1.00g
・MEL-B・・・0.10g
Claims (8)
- マンノシルエリスリトールリピッド(MEL)を含有することを特徴とする汚染防止剤。
- マンノシルエリスリトールリピッド(MEL)がMEL-Bである請求項1に記載の汚染防止剤。
- R1の炭素数が8~14個である請求項3に記載の汚染防止剤。
- マンノシルエリスリトールリピッド(MEL)が溶媒中に分散・溶解して成る組成物である請求項1から4のいずれかに記載の汚染防止剤。
- 請求項1から5のいずれかに記載の汚染防止剤を含む皮膚外用剤。
- マンノシルエリスリトールリピッド(MEL)を0.001~10.0質量%含有する請求項6に記載の皮膚外用剤。
- 請求項1から5のいずれかに記載の汚染防止剤を用いることにより大気エアロゾル粒子の皮膚または毛髪への付着を抑制する方法。
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