WO2018084275A1 - System and method for cultivating araliaceae medicinal plants - Google Patents

Abstract

The purpose of the invention is to provide a fertilizer for Araliaceae plants that is capable of efficiently increasing the content of specific ginsenosides and shortening the cultivation period. Disclosed is a system for cultivating Araliaceae plants, said system comprising a means that emits violet light (1) and a means that emits red light (2), wherein the intensity of the red light (2) is within a range from 3 to 30 with respect to an intensity of 1 of the violet light (1). Also disclosed is a method for cultivating Araliaceae medicinal plants, said cultivation method comprising a step for emitting violet light (1) and a step for emitting red light (2), wherein the intensity of the red light (2) is within a range from 3 to 30 with respect to an intensity of 1 of the violet light (1).

Description

Urugiaceae medicinal plant cultivation system and cultivation method

The present invention relates to a cultivation system and a cultivation method for a medicinal plant.

Ginseng (Ginseng) is also known as Korean ginseng and Panax ginseng, and is a perennial plant belonging to the family Araceae that has been used for medicinal purposes or for food. For example, the newly sprouted ginseng is used as a medicinal dish in addition to one of the traditional Korean dishes, samgyetang, and eats not only the roots of ginseng but also stems and leaves. be able to.

The main useful ingredient contained in Korean ginseng is a ginseng saponin group called ginsenoside, and this ginsenoside may have various functions such as antioxidant action, blood pressure promoting action, and anti-inflammatory action. In recent years, Korean ginseng has attracted attention not only in Korea but also around the world.

There are more than 20 types of ginsenosides in Ginseng known as F1, F2, Rc, Rb1, Rb2, Rb3, Rc, Rd. And it has become clear that every various ginsenoside has an interesting physiological activity (patent document 1).

Open field cultivation of Korean ginseng usually requires a cultivation period of at least about 4 to 6 years from sowing to harvesting and is very difficult to cultivate because it is vulnerable to pests. It is known that continuous production is difficult.

Therefore, research on efficient cultivation methods for Korean ginseng has been actively conducted so far. For example, Patent Literature 2 discloses a cultivation method in which control is performed under specific cultivation conditions from sowing to harvesting.

The size of the roots of the Korean ginger is important, but it is judged that the quality of the long and thin stems will deteriorate. Therefore, the cultivation method for ginseng with short and thick stems is required.

However, it is still not sufficient as a method for cultivating these ginseng pods, and establishment of a method for efficiently cultivating ginseng pods containing a lot of specific ginsenosides is desired.

JP 2014-015462 A International Publication No. 2015/093607

The present invention has an object to provide a cultivation system and a cultivation method for a medicinal plant that can increase the content of ginsenoside, have good quality, and shorten the cultivation period.

As a result of intensive studies to solve the above problems, the present inventor has means for irradiating purple light and means for irradiating red light, and the intensity of the red light is set to an intensity of purple light 1. On the other hand, it was found that the content of a specific ginsenoside in the Argiaceae medicinal plant can be increased by setting the content in the range of 3 to 30. The present invention has been completed based on such findings.

That is, the present invention relates to a cultivation system and a cultivation method for a medicinal plant.
Item 1.
It is a cultivation system for araceae plants,
(1) means for irradiating purple light, and (2) means for irradiating red light, and (2) the intensity of the red light is 3 to 30 with respect to (1) the intensity of purple light 1. The cultivation system, which is a range.
Item 2.
(1) The cultivation system according to item 1, wherein the violet light has a peak in a wavelength range of 380 to 450 nm.
Item 3.
(2) The cultivation system according to item 1 or 2, wherein the red light has a peak in a wavelength range of 620 to 750 nm.
Item 4.
Item 4. The cultivation system according to any one of Items 1 to 3, wherein (2) the intensity of red light is in the range of 3.2 to 10 with respect to (1) the intensity of violet light 1.
Item 5.
Item 5. The cultivation system according to any one of Items 1 to 4, wherein the content of ginsenoside in the Argiaceae plant can be increased.
Item 6.
The ginsenoside is ginsenoside F1, ginsenoside F2, ginsenoside F5, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rh2 (S), ginsenoside Rh1 Item 6. The cultivation system according to Item 5, which is at least one ginsenoside selected from the group consisting of R).
Item 7.
Item 7. The cultivation system according to any one of Items 1 to 6, wherein the Araceae plant belongs to the genus Tochibaninjin.
Item 8.
Item 8. The cultivation system according to any one of Items 1 to 7, further comprising means for applying fertilizer.
Item 9.
Item 9. The cultivation system according to any one of Items 1 to 8, further comprising means for ultrasonically spraying the fertilizer.
Item 10.
A method for cultivating medicinal medicinal plants, comprising (1) a step of irradiating violet light, and (2) a step of irradiating red light, and (2) the intensity of the red light is (1) The cultivation method as described above, which is in the range of 3 to 30 with respect to the strength 1.
Item 11.
(1) The cultivation method according to item 10, wherein the violet light has a peak in a wavelength range of 380 to 450 nm.
Item 12.
(2) The cultivation method according to item 10 or 11, wherein the red light has a peak in a wavelength range of 620 to 750 nm.
Item 13.
Item 13. The cultivation method according to any one of Items 10 to 12, further comprising a step of applying a fertilizer.
Item 14.
Item 14. The cultivation method according to any one of Items 10 to 13, further comprising the step of ultrasonically spraying the fertilizer.
Item 15.
Item 15. A plant composition obtained from the cultivation system according to any one of Items 1 to 9 or the cultivation method according to any one of Items 10 to 14.
Item 15-1.
Item 15. An Araceae plant obtained by the cultivation system according to any one of Items 1 to 9, or the cultivation method according to any one of Items 10 to 14.
Item 16.
Item 20. An argycaceous plant composition according to item 15, which satisfies at least one of the following conditions A) to C).
A) The content of ginsenoside F2 contained in the harvest is 0.1 mg or more / live weight 1 g,
B) The content of ginsenoside Rg2 (S) contained in the harvest is 0.05 mg or more / live weight 1 g,
C) Ginsenoside Rf content contained in the harvest is 0.05 mg or more / live weight 1 g.
Item 17.
Item 15. A processed product comprising the Araceae plant composition according to Item 15 or 16.
Item 18.
Item 15. An extract of the Argiaceae plant composition according to Item 15 or 16.
Item 19.
Item 10. The cultivation system according to any one of Items 1 to 9, which suppresses the length of a stem of the Argiaceae plant.
Item 20.
Item 10. The cultivation system according to any one of Items 1 to 9, which increases the thickness of a stem of an Araceae plant.
Item 21.
Item 15. The cultivation method according to any one of Items 10 to 14, wherein the length of a stem of the Argiaceae plant is suppressed.
Item 22.
Item 15. The cultivation method according to any one of Items 10 to 14, wherein the thickness of a stem of the Argiaceae plant is increased.

According to the present invention, the content of a specific ginsenoside in the Argiaceae plant can be remarkably increased.

According to the present invention, it is possible to cultivate Araceae plants with good quality, thick stems and short.

In addition, according to the present invention, it is possible to cultivate a sprout Korean ginseng in about 20 to 30 days, where conventional outdoor cultivation takes about one year.

Thus, since it can be shortened compared to the cultivation period of the conventional medicinal plant, it is possible to dramatically improve the production efficiency and to provide the medicinal plant at low cost.

FIG. 1 is obtained by the Korean vine (25th day) obtained by the cultivation system and cultivation method (Examples 1 and 2) of the medicinal plant of the present invention, and the conventional cultivation method of Comparative Example 1. This is a comparative photograph of the Korean people. FIG. 2 is a photograph of a Korean ginseng after being cultivated with the fertilizer of Comparative Example 1 in each cultivation period (after 50 days, 70 days, 90 days, and 115 days). FIG. 3 is a photograph of a Korean ginseng after being cultivated for 25 days by the cultivation system and cultivation method (Examples 1 and 2) of the medicinal plant of the present invention. FIG. 4 is a schematic diagram of the cultivation system (isolated bed soil cultivation) of the present invention. FIG. 5 is a schematic diagram of the cultivation system of Comparative Example 1 (cultivation employing ultrasonic spraying means). FIG. 6 is a schematic diagram of the cultivation system of the present invention (cultivation employing LED light irradiation means and ultrasonic spray means).

Hereinafter, the cultivation system and cultivation method of the present invention will be described in detail.

Cultivation system The cultivar system of the present invention (hereinafter sometimes referred to as “the cultivation system of the present invention”) includes means for irradiating purple light and means for irradiating red light, and the red light. The intensity of the light is in the range of 3 to 30 with respect to the intensity 1 of violet light.

The violet light has a peak in the wavelength range of 380 to 450 nm, preferably 400 to 445 nm, and more preferably 420 to 440 nm.

The red light has a peak in the wavelength range of 620 to 750 nm, preferably 700 to 740 nm, and more preferably 710 to 730 nm.

The light irradiation means is not particularly limited, and examples thereof include (1) means for applying purple illumination light and means for applying red irradiation light.

(2) The intensity of red irradiation light is preferably 3.2 to 10, more preferably 1: 3.5 to 8, particularly preferably 3.8 to 4, with respect to (1) the intensity of purple illumination light 1. .2 is preferable.

The purple illumination light and the red irradiation light may be irradiated simultaneously or separately (alternately), and the simultaneous irradiation is preferable. The wavelengths of violet light and red light can be changed within the above wavelength range.

Light intensity (intensity)
The amount (intensity) of the violet illumination light and the red illumination light is not particularly limited. For example, the photosynthesis flux density (PPFD) is 1 to 1000 μmol / m ² s, preferably 10 to 500 μmol / m. ² s, particularly preferably about 20 to 250 μmol / m ² s. In addition, as this PPFD, the light quantity of the distance of about 30 cm from a light source can be measured using a general photon meter.

The light intensity (intensity) ratio of the violet illumination light and the red illumination light is, for example, “purple: red” and is 1: 3 to 30, preferably 1: 3.2 to 10, more preferably 1: 3. It is in the range of 5 to 8, particularly preferably 3.8 to 4.2. The amount of violet illumination light and red illumination light can be varied within the above range.

Irradiation time LED light irradiation time is the longest cultivation period. The shortest time can be arbitrarily set as long as the effect of the present invention is exhibited.

The light irradiation unit includes a light source that emits purple light or red light. A conventionally well-known light source can be used individually or in combination for the light source of purple light and red light.

As the light source, it is preferable to use an optical semiconductor element such as a light emitting diode (LED) or a laser diode (LD) that emits light that allows easy wavelength selection and a large proportion of light energy in the effective wavelength region. Further, when electroluminescence (EL) is used as the light source, EL may be organic or inorganic.

Opt-semiconductor element is small and has a long life, and it emits light at a specific wavelength depending on the material, so there is no unnecessary heat radiation, so energy efficiency is good, and even if it is irradiated close to a plant, it does not cause damage such as burning of leaves. For this reason, it becomes possible by using an optical semiconductor element for a light source to cultivate at a lower power cost and more space-saving than other light sources.

The cultivation system of the present invention can include means for applying fertilizer.

The fertilizer used in the cultivation system of the present invention is not particularly limited, and examples thereof include silicate. The silicate is not particularly limited, and examples thereof include an alkali metal silicate or a hydrate thereof; an alkaline earth metal silicate or a hydrate thereof, and the like. Among them, the silicate is preferably a water-soluble silicate (sometimes referred to as water-soluble silicon), more preferably an alkali metal silicate or a hydrate thereof, and a hydrate of an alkali metal silicate. Is more preferable.

The alkali metal silicate is not particularly limited, for _{example, Na 2 SiO 3, Na 4} SiO 4, Na 2 Si 2 O 5, Na 2 Si 4 sodium silicate O _9, etc.; K ₂ SiO _3, K ₄ Formulas such as potassium silicate such as SiO ₄ , K ₂ Si ₂ O ₅ , K ₂ Si ₄ O _9, etc .: m (M ₂ O) · n (SiO ₂ ) (where m and n are positive integers) And M represents an alkali metal atom.).

The number of hydrates of the alkali metal silicate is not limited, and the monohydrate, dihydrate, trihydrate, tetrahydrate, and 5 water of the alkali metal silicate described above. Japanese hydrates, hexahydrates, heptahydrates, octahydrates, 9 hydrates, 10 hydrates, 11 hydrates and the like can be mentioned. Of these, sodium silicate decahydrate (Na ₂ SiO ₃ .10H ₂ O) is preferable.

Na ₂ SiO ₃ · 10H ₂ O is a commercially available product or, for example, a crystal (KR10-) in which quartz (quartz) is burnt and dissolved at a high temperature (about 1,650 ° C or higher) for 8 hours or more to decompose unnecessary components. 0361045 or the method described in KR10-415594) can be used.

The alkaline earth metal silicate is not particularly limited, and examples thereof include calcium silicate such as a formula: 2CaO · xSiO ₂ (where 1 <x <2).

The hydrate of the alkaline earth metal silicate is not limited to the number of hydrates, and the monohydrate, dihydrate, trihydrate, tetrahydrate of the alkali metal silicate described above, Examples include pentahydrate, hexahydrate, heptahydrate, octahydrate, nonahydrate, decahydrate, and eleven hydrate.

Silicates can be used singly or in combination of two or more.

The silicate can be used in the form of a solid (crystal, granule, powder, etc.), or an aqueous solution obtained by dissolving the solid in a solvent such as water or ethanol can be used.

The concentration of the aqueous solution is not particularly limited, and examples thereof include a range of 0.001 to 20000 ppm. Specifically, as a usage form of the cultivation system or cultivation method of the present invention, solid silicate is dissolved in water to produce a concentrated solution type (for example, 5000 ppm to 20000 ppm), and the concentrated solution is actually used as a fertilizer. Can be used by appropriately diluting with water or the like.

The fertilizer used in the cultivation system of the present invention may be composed only of the silicate (or a hydrate thereof), but may contain a known fertilizer other than the silicate.

The known fertilizer is not particularly limited as long as it is a fertilizer component other than the silicate, and examples thereof include inorganic fertilizers (chemical fertilizers) and organic fertilizers.

Examples of inorganic fertilizers include nitrogenous fertilizers (lime nitrogen; urea; inorganic acid ammonium salts such as ammonium sulfate, ammonium chloride, and ammonium nitrate; alkali metal salts or alkaline earth metal salts of nitric acid such as sodium nitrate and potassium nitrate); phosphoric acid Fertilizer (alkali metal salt or alkaline earth metal salt of phosphoric acid such as superphosphate lime, heavy superphosphate, molten phosphorus fertilizer, calcined phosphorus fertilizer); Caliper fertilizer (potassium carbonate, potassium chloride, potassium sulfate) Compound potassium phosphate fertilizer (eg, monopotassium phosphate, dipotassium phosphate, etc.); siliceous fertilizer (eg, calcium silicate); magnesiumaceous fertilizer (eg, magnesium sulfate, magnesium chloride, etc.); Zinc fertilizer (eg, zinc sulfate, zinc, etc.); Calcium fertilizer (eg, quicklime, slaked lime, calcium carbonate, etc.) Manganese fertilizer (for example, manganese sulfate, sulfated manganese clay); boron fertilizer (for example, boron, boric acid, borate, etc.); fertilizer containing fertilizer (for example, chelated iron (ethylenediaminetetraacetic acid iron complex: EDTA- Fe), steel slag, etc.); copper fertilizers (for example, copper sulfate, etc.); ordinary fertilizers (including compound fertilizers) defined in the Fertilizer Control Law such as molybdenum fertilizers (for example, sodium molybdate).

As the organic fertilizer, for example, any organic fertilizer may be used as long as it contains organic nitrogen such as protein or a decomposition product thereof, amino acid and ammonia. Specific examples of the organic fertilizer include organic fertilizers such as compost, green manure, blurred fertilizer, and litter; food residues such as fish meal, oil cake, okara, raw garbage, rice bran, and extracts or concentrates obtained therefrom; Organic wastes such as Inawara, and waste water containing these organic substances are listed.

Known fertilizers can be used alone or in combination of two or more.

When a known fertilizer is added to the silicate fertilizer, the amount added is not particularly limited. For example, the amount is usually 0.001 to 10000 parts by mass, preferably 0. The amount is about 01 to 1000 parts by mass, more preferably about 0.1 to 500 parts by mass.

In addition, the fertilizer further includes physiologically active substances (for example, growth regulators such as growth promoters and growth inhibitors), microbial material extracts, agricultural chemicals (for example, weeding) to the extent that the effects of the present invention are not impaired. Agents, insecticides, fungicides, acaricides, nematicides, etc.), surfactants (for example, nonionic surfactants, anionic surfactants, carboxylic acid surfactants, sulfonic acid surfactants) , Sulfate ester surfactants, phosphate ester surfactants, amphoteric surfactants, etc.), vitamins (eg, vitamin B1, vitamin B6, nicotinamide, choline salts, etc.), antiseptic agents (eg, benzoic acid) Acid sodium, sorbic acid, propionic acid, etc.), chelating agents (eg, ethylenediaminetetraacetic acid or salts thereof; citric acid or salts thereof (eg, sodium salts, potassium salts, etc.)), pH adjusters, Gluteal agents, spreading agents, other components such as coloring agents may be added.

When other components are used, the amount used is usually 0.001 to 10000 parts by weight, preferably 0.01 to 1000 parts by weight, more preferably 0.1 to 500 parts by weight with respect to 100 parts by weight of the silicate. About mass parts.

Other components can be used alone or in combination of two or more.

The content of the silicate ions in the fertilizer is not particularly limited, for example, not more than 20 wt% in terms of SiO ₂ is preferably 10% by mass, more preferably 5 mass%. Moreover, as a minimum of content of the said silicate ion, 0.001 mass% is preferable, 0.01 mass% is more preferable, 0.1 mass% is further more preferable.

The cultivation system of the present invention can further include ultrasonic spraying means. In addition, the cultivation system of the present invention can employ known cultivation means used in plant factories.

The ultrasonic spraying means is not particularly limited, and a known or commercially available ultrasonic spraying apparatus can be used. There are no particular limitations on the conditions such as the spray amount per spray time of the liquid fertilizer, the spray time, and the spray interval.

Cultivation Method The cultivation system of the present invention can be used in a method for cultivating Argiaceae plants (hereinafter sometimes referred to as “cultivation method of the present invention”). The cultivation method of the present invention comprises (1) a step of irradiating purple light, and (2) a step of irradiating red light, and (2) the intensity of red light is (1) the intensity of purple light 1 On the other hand, it may have steps that range from 3 to 30, and may further comprise a step of applying fertilizer (process) and / or a step of ultrasonically spraying the fertilizer (process).

The cultivation method of the present invention can include a step of applying a fertilizer. The form (shape) of the fertilizer is not particularly limited. For example, any known form of fertilizer such as powder, granule, paste, slurry, suspension, and solution can be used. . The fertilizer can be diluted to a desired concentration with an agriculturally acceptable solvent or carrier to give a liquid fertilizer.

Agriculturally acceptable solvents include water (including sterilized water, deionized water, and ultrapure water) or other agriculturally acceptable aqueous solutions. Examples of the aqueous solution include a buffer such as a phosphate buffer and a liquid medium.

The agriculturally acceptable carrier includes the above-mentioned other components. When silicate is used as the fertilizer, it is preferably an aqueous solution, and the concentration of silicate in the aqueous solution is not particularly limited, and is usually 0.001 to 200000 ppm, preferably 0.01 to 150,000 ppm, more preferably 0.1 to 100,000 ppm.

The fertilizer application method is not particularly limited, and a method similar to a general fertilizer application method can be used. For example, when the fertilizer is a liquid fertilizer, a method of spraying or irrigating the liquid fertilizer on soil; a method of spraying the liquid fertilizer on the leaves of crops; a method of drip irrigation of the liquid fertilizer; A method of cultivating the cultivation; a method of spray-cultivating the liquid fertilizer, and the like.

According to the plant cultivation method of the present invention, it is possible to reduce the cultivation cost in the cultivation of Argiaceae plants. In particular, in an artificial medium cultivation method such as hydroponics or spray cultivation, an inexpensive cultivated plant can be provided by suppressing power costs and fertilizer costs.

The cultivation method of the present invention includes a step of applying the fertilizer of the present invention to an Argiaceae plant. About an application method, it can select suitably according to the kind, etc. of soil and a araceae plant. For example, the fertilizer can be applied to the cultivated soil, preferably the corresponding part of the main root group of the target crop by mixing, spraying, irrigation and the like. There is no limitation in particular as the cultivation method of this invention, For example, the said fertilizer is applicable to isolation bed soil culture cultivation, hydroponics cultivation, outdoor cultivation, etc.

In this specification, “isolated bed soil cultivation” means adding culture soil (floor soil, masa soil, bark, coconut culture soil, peat moss, perlite, etc.) to an isolated cultivation container (bed) and instilling the nutrient solution. Or it is the method of irrigating and growing a crop.

In this specification, “hydroponics” is a cultivation method performed in a state where all or a part of the roots of the cultivated plant is immersed in a hydroponic liquid, for example, a bottom irrigation method, a bubbling method, a spray (mist) method. Etc. The bottom irrigation method is a method of cultivating by supplying water or nutrient solution to the plant by filling the bottom surface of the re-incubator with water or nutrient solution. The bubbling method is a method of cultivating by supplying water or nutrient solution containing air bubbles to the underground part of the plant. A spraying method is a method of cultivating by spraying water or nutrient solution on the underground part of a plant.

In the present specification, “spray cultivation” includes, for example, a method of spraying in a mist form. Examples of such spraying methods include a spray type using high-pressure gas, ultrasonic spraying (ultrasonic mist), and the like. The ultrasonic spray is not particularly limited as long as it is a known ultrasonic spray used in plant cultivation.

As the spray cultivation, an ultrasonic spray method is preferable in that a specific component in the araceae plant is increased or the growth of the araceae plant is promoted.

The cultivation method of the present invention may further include an LED light irradiation step. The cultivation method of the present invention can be applied to a plant factory, particularly a fully controlled plant factory. Here, a “fully controlled plant factory” means that in a closed space like a building, weather conditions such as light, humidity, temperature, etc., medium supply or exchange, etc. are completely systematized, and computer controlled It means a factory that cultivates plants in a man-made environment. In plant factories, hydroponic cultivation is generally adopted from the viewpoints of management, hygiene, labor, and the like.

In normal hydroponics, it is cultivated only with hydroponic liquid, but in hydroponic culture in this specification, the hydroponic liquid can be filled with a support as a plant scaffold. For the support, for example, inorganic materials such as urethane, rock wool, sand, gravel, vermiculite, pearlite; sawdust, rice husk, coconut shell, bark chip, floor soil, masa soil, bark, coconut culture soil, peat moss, agar Natural organic materials such as; or combinations thereof can also be used.

Conditions known in the art with respect to cultivated plants can be applied to the weather conditions including light and dark times (light irradiation time and dark time), temperature, humidity and the like, and the growing conditions such as the growing period in the plant factory. Especially, it is preferable that the cultivation method of this invention has an ultrasonic spraying process and an LED light irradiation process.

Argiaceae plant (or Argiaceae plant composition)
The araceae plant obtained by the cultivation system or the cultivation method of the present invention (hereinafter also referred to as “the urchinaceae plant obtained by the present invention”) contains ginsenoside. Among them, ginsenoside includes ginsenoside F1, ginsenoside F2, ginsenoside F5, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rg2 (S), ginsenoside R, and ginsenoside R It contains at least one selected from the group consisting of Rh1 (R).

The Araceae plant obtained by the present invention is at least the following A) to C):
A) The content of ginsenoside F2 contained in the harvest is 0.1 mg or more / live weight 1 g,
B) The content of ginsenoside Rg2 (S) contained in the harvest is 0.05 mg or more / live weight 1 g,
C) The ginsenoside Rf content contained in the harvest contains 0.05 mg or more / live weight 1 g.

The content of ginsenoside in the Argiaceae plant can be increased by the cultivation system or cultivation method of the present invention.

Ginsenoside, for example, is a unique saponin group contained in Korean ginseng, and there are more than 20 kinds, and it is known to have various physiological activities such as antioxidant action, blood circulation promoting action, etc. Yes.

Examples of ginsenoside include protopanaxadiol type ginsenoside [eg, Rb1, Rb2, Rc, Rd, (20R) Rg3, (20S) Rg3, Rh2], protopanaxatriol type ginsenoside [eg, Re, Rf, Rg1 , Rg2, Rh1], and oleanolic acid type ginsenoside [eg, RO]. Among them, in the cultivation system and cultivation method of the present invention, ginsenoside F1, ginsenoside F2, ginsenoside F5, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rg, ginsenoside Rg1, ginsenoside Rg2 The content of at least one ginsenoside selected from the group consisting of S), ginsenoside Rh1 (S), and ginsenoside Rh1 (R) can be increased. Nutrients (Ginsenoside Rf, Rg2s, F2, etc.) that are not included in the sprout cultivated without adding silicate to the sprout (young sprout) human pods cultivated with the addition of fertilizer containing the silicate of the present invention Is included. For example, ginsenoside F2 is a component that has recently attracted attention for its physiological activity such as the effect of preventing or treating atopic dermatitis (Patent Document 1).

The cultivation system or cultivation method of the present invention can suppress the length of the stem of the Araceae plant or increase the thickness of the stem of the Argiaceae plant. In the araliaceae plant, there are underground parts such as roots, rhizomes and tubers, and ground parts such as leaves, petioles, stems, fruit stems and flowers, but according to the cultivation system or cultivation method of the present invention, the usual cultivation method As compared with, the stems of Argiaceae plants are thick and can be cultivated with a short length. It is assumed that the content of the ginsenoside is increased due to such characteristics.

There are no particular limitations on the aragonaceae plant, for example, the genus Taranoki, Udo, etc., the genus Ucogi, Kosiabura, etc., the genus Takatsuki, such as Takatsutsu, the genus Azalea, etc. Examples include the genus Tochibaninjin such as Tochibaninjin. Among these, the genus Araceae is preferably the genus Tochibanin, which is a medicinal plant, and more preferably Korean ginseng (Korean ginseng, Panax ginseng). In addition, the carrot of a vegetable is an Aceraceae and is a completely different kind from an Argiaceae plant.

There are no particular limitations on the types of Korean ginseng, and examples include Hanafang San (United States Ginseng), Tanachi ginseng (Chinese Ginseng), Takebushi Ginseng (Japanese Ginseng) and the like. In addition, these ginseng can produce processed products such as white ginseng, red ginseng and black ginseng by a normal drying process.

Processed products The Argiaceae plants obtained by the cultivation system or cultivation method of the present invention are processed into various processed products such as extracts, powders, granules, granules, tablets, capsules, gels, and liquids. Can do. As a processing method for these processed products, a known processing technique can be used. The processed product thus obtained can be produced as it is or by containing other components, for example, to produce foods or pharmaceuticals with enhanced bioregulatory functionality.

The extract extract means a product obtained by extracting an Argiaceae plant with a solvent such as water or ethanol. Especially, as an extract, what was extracted with the solvent (solution) containing the said silicate or its hydrate is preferable.

For example, leaves, stems, roots, and the like can be used as the part of the Argiaceae plant used for extraction. The arboreal silicate extract of the present invention can be extracted using all (leaves, stems, and roots) or a part of the above-mentioned parts of the Argiaceae plant. Especially, it is preferable to extract the whole said site | part of the Argiaceae plant at the point which is easy to manufacture or contains many kinds of active ingredients.

The extraction method is not particularly limited. For example, aragonaceae plants or their respective parts as they are, or cut or crushed, dried, dried and crushed, squeezed and extracted juice, etc. Extraction method combining immersion, stirring, heating, etc. in a solvent in which is dissolved; supercritical fluid extraction method and the like.

The solvent (solution) containing a silicate or a hydrate thereof is not particularly limited as long as it is a solvent generally used for obtaining an extract. For example, water; ethanol, n-propyl alcohol, isopropyl alcohol, n- Examples include lower alcohol solvents such as butyl alcohol; organic solvents such as ester solvents such as methyl acetate, ethyl acetate, butyl acetate, isobutyl acetate, and propyl acetate. These solvents can be used alone or in combination of two or more. When combining, extraction operation can be performed using a mixture of a plurality of solvents, or extraction operation can be performed in multiple stages in order with different solvents. Among the solvents, water and ethanol are preferably used, and water is particularly preferable from the viewpoints of operability, safety and environmental properties. In the present invention, by using an aqueous silicate solution, it is possible to increase the amount of the extract of Araceae and greatly improve the extraction speed.

The amount of the solvent at the time of extraction is not particularly limited, and is, for example, 1 to 10000 parts by mass of water, preferably 5 to 1000 parts by mass, more preferably 100 parts by mass of Argiaceae plants. 10 to 500 parts by mass.

When an aqueous solution of silicate is used, the concentration of the silicate is not particularly limited, and is, for example, 0.1 ppm to 200000 ppm, preferably 1 ppm to 100,000 ppm, and more preferably 10 to 50000 ppm.

The amount of silicate is not particularly limited and is, for example, 1 to 10000 parts by mass, preferably 50 to 1000 parts by mass, more preferably 100 to 500 parts per 100 parts by mass of Araceae plants. Part by mass.

When extracting, it can be extracted by heating or pressurizing / heating. When pressurizing, the pressure is not particularly limited, and is selected, for example, in the range of 1.01 to 5 MPa. In the case of heating, the temperature may be room temperature or higher, preferably 60 ° C. or higher, and more preferably 100 ° C. or higher.

The extraction time is not particularly limited and is, for example, in the range of 1 to 24 hours, preferably 2 to 18 hours, more preferably 3 to 12 hours.

Next, the mixture containing the extract and the residue is subjected to filtration, centrifugation, or the like as necessary, and the solid component that is the residue is removed to obtain an extract. Note that the removed solid component can be subjected to the extraction operation again, and this operation can be repeated several times. In the present invention, the extraction step can be performed once, but can be extracted in two or more steps. Different extraction conditions may be used for two or more extractions.

The extract (or extract) thus obtained may be used as it is, and may be used as a powder after drying by a method such as concentration, freeze-drying or spray-drying, if necessary. Good.

Hereinafter, the present invention will be specifically described with reference to Examples and Comparative Examples, but the present invention is not limited thereto.

Light source of light irradiation means The LED of the following light source 1 was used as the light source for the light environment used in the cultivation system and cultivation method of the medicinal plant of the present invention.
Light source 1
・ Purple LED (Center wavelength: 430 nm): Red LED (Center wavelength: 720 nm)
A light source having a red light intensity of about 4 with respect to a purple light intensity of 1 was used.
・ PPFD: 220μmol / m ² sec (distance from light source 30cm)
・ Operating voltage: 24V / 28.8W
・ 600mA constant current method / 30W class high power LED
In addition, as a light source used in the comparative example 1, LED of the following light source 2 was used.
Light source 2
LED (center wavelength: 450 nm): LED (center wavelength: 660 nm)
A light source having a red light intensity of about 2 with respect to a purple light intensity 1 was used.
・ PPFD: 200μmol / m ² sec (distance from light source 30cm)
・ Operating voltage: 24V / 28.8W
・ 600mA constant current method / 30W class high power LED.

Cultivation system: Isolation bed soil cultivation system As shown in FIG. Pipe (FRP or iron), 2. Support pipe, 3. 3. Nonwoven fabric, 4. Polyethylene (PE) film tunnel, Cultivation bed, 6. 6. Infusion hose, Seedlings, 8. Black PE film, 9. 9. Straw foam bed Garden floor soil, 11. Rice husks, 12. Black PE film, 13. Drains, and 14. Has a coaster pipe.

Cultivation system: spray tillage cultivation method As shown in FIG. Pipe (FRP or iron), 16. Non-woven fabric, 17. Vinyl tunnel, 18. Iron struts, 19. Cultivation bed, 20. Seedlings, 21. Fixing sponge, 22. Bed top plate, 23. Supply pipe, 24. Drainage channel, 25. Injection nozzle (M-Techwin Co., Ltd., product name: MH-106A (6 wards), humidification ultrasonic type, power consumption: 240W, maximum spray amount: 2,800 ± 200 (approx. 3000cc) / hr, water method: Automatic water supply method, water pressure used: 0.2 to 6.8 bar, use area about 30 tsubo, external dimensions: 516 (width) × 270 (length) × 284 (height)), and 26. It has a nonwoven fabric.

Cultivation system: Ultrasonic spray cultivation method In spray cultivation, since rhizosphere oxygen may be deficient, ultrasonic spray cultivation is useful as a means to solve it.
As shown in FIG. 6, the cultivation system using the light irradiation means using LED and the ultrasonic spray means is 27. LED, 28. Fog spray nozzle, 29. Vinyl cover, 30. Koryojin, 31. Cultivation bed top plate, 32. Cultivation bed, 33. Ultrasonic spray pump, 34. Pipes, and 35. Has a fixing sponge.

Conditions for HPLC analysis Ultra-high performance liquid chromatography system: LaChromUltra L-2000 U series (manufactured by Hitachi High-Technologies)
The apparatus includes an eluent reservoir, an HPLC pump (L-2160U), an automatic injection system (L-2200U), and an ultraviolet detector (L-2400U).

Column: LaChromUltra C18 short-length column (2mm id, 50mm L, 2μm; manufactured by Hitachi High-Technologies), LaChromUltra C18 middle-length column (2mm id, 100mm L, 2μm; manufactured by Hitachi High-Technologies)
Eluent A: 20% acetonitrile aqueous solution, eluent B: 80% acetonitrile aqueous solution Column temperature: 30 ° C
Detection: Varian 380-LC
Injection volume: 5.0μL
Gradient: A / B = 100/0 → 0/100 (10 min); Flow rate: 0.2 mL / min.

Fertilizer <Fertilizer 1>
Silicate (Na ₂ SiO ₃ -10H ₂ O) crystals 500 g (manufactured by KOSIBIO Co., Ltd) were dissolved in 50 liters of water to prepare liquid fertilizer 1 (10,000 ppm) of the present invention.

<Reference fertilizer>
(Fertilizer A)
Fertilizer solution 1 (500ml, DAEYU Co., Ltd) containing 1-4 below.
1. Calcium nitrate 20g
2. 30g of glass
3. Chelated iron 2.5g
4). About 470cc water

(Fertilizer B)
Fertilizer solution 2 containing the following 1-8 (100ml, DAEYU Co., Ltd).
1. Monobasic magnesium phosphate 4.5g
2. Magnesium sulfate 12.5g
3. Boron or boric acid 150mg
4). Manganese 100mg
5). Zinc 10mg
6). Copper 5mg
7). Sodium molybdate 1mg
8). Water about 93cc

(Fertilizer C)
Liquid fertilizer A (500 g), liquid fertilizer B (100 g), and water (50 L) were mixed together and stirred well to prepare liquid fertilizer C (50600 g) containing the following components 1 to 10.
1. Calcium nitrate (Ca (NO ₃ ) ₂ · 4H ₂ O) 4g,
2. 6 g of potassium nitrate (KNO ₃ )
3. Chelated iron (ethylenediaminetetraacetic acid iron complex: EDTA-Fe) 0.5g,
4). 0.9 g of primary ammonium phosphate (NH ₄ · H ₂ PO ₄ )
5). Magnesium sulfate (MgSO ₄ · 4H ₂ O) 2.5 g,
6). Boron (B) or boric acid (H ₃ BO ₃ ) 0.03g,
7). Manganese (MnSO ₄ · 4 _H2 O) 0.02g,
8). Zinc (ZnSO ₄ · 4H ₂ O) 0.005g,
9. Copper (CuSO ₄ · 5H ₂ O) 0.002g,
10. Sodium molybdate (NaMoO ₄ · 2H ₂ O) 0.001 g.

<Fertilizer 2>
Sodium silicate decahydrate (50 g) and the above liquid fertilizer C (50600 g) were mixed together to prepare silicate and liquid fertilizer 2 (50650 g).

<Fertilizer 3>
The liquid fertilizer C was used as fertilizer 3.

[Comparative Example 1]
(Hydroculture; spray cultivation method)
1 year old seedlings of the Korean ginseng are arranged at regular intervals (about 4 cm) on the cultivation top plate bed, and the above spray spray (not ultrasonic) is placed in the basement and the LED lamp (light source 2) is placed on the ground. (FIG. 5). While irradiating light using LED of the said light source 2, the said fertilizer 3 was sprayed and it grew.

The components of Korean ginseng cultivated in Comparative Example 1 were analyzed, and the results of the amount of each component of ginsenoside are shown in Table 1 and Table 2.

[Example 1]
(Soil cultivation; isolated bed cultivation method)
The annual seedlings of the Korean ginseng were planted at regular intervals (about 4 cm) on the soil of an isolation bed (FIG. 4). The fertilizer 1 was used to cultivate the Korean soil in the soil cultivation method under the light irradiation of the LED (light source 1).

The components of Ginseng cultivated by the method described in Example 1 were analyzed, and among them, the results of the amount of each component of ginsenoside are shown in Table 1.

<Cultivation result>
The cultivation system and cultivation method described in Comparative Example 1 usually requires a cultivation period of about 60 to 90 days from the implantation of the ginseng to the collection, whereas the cultivation system and the cultivation method described in Example 1 The cultivation method was able to grow quickly in the cultivation period of 25 days, and the production efficiency improved dramatically.
Moreover, as is clear from the results in Table 1, the ginseng cultivated using the cultivation system and the cultivation method of the present invention is more ginsenoside than the ginseng cultivated by the method described in the conventional comparative example 1. The content of each increased significantly. As shown in FIG. 1, the stem A of Korean ginseng cultivated using the cultivation system and cultivation method of the present invention is the same as the stem B of Korean ginseng grown using the method described in the conventional Comparative Example 1. In comparison, the stems were short and thick, and according to the present invention, Korean ginseng, which is considered to have high quality, could be harvested.

[Example 2]
(Hydroculture; ultrasonic spray cultivation method)
On the cultivated top plate, the annual seedlings of the Korean ginseng are arranged at equal intervals (about 4 cm), and the ultrasonic spray and the LED lamp (light source 1) are arranged in the basement (FIG. 6). ). Using the fertilizer 2 of the present invention, cultivation of Korean ginseng using an ultrasonic spray plowing method was performed under the irradiation of the LED light.

The components of ginseng cultivated by the method described in Example 2 were analyzed, and among them, the results of the amount of each component of ginsenoside are shown in Table 2.

<Cultivation result>
The cultivation system and cultivation method described in Comparative Example 1 usually requires a cultivation period of about 60 to 90 days from the implantation of the ginseng to the collection, whereas the cultivation system and the cultivation method described in Example 2 The cultivation method was able to grow quickly in the cultivation period of 25 days, and the production efficiency improved dramatically.
Moreover, as is clear from the results in Table 2, Korean ginseng cultivated using the cultivation system and cultivation method described in the present invention was cultivated using the cultivation system and cultivation method described in the conventional Comparative Example 1. Compared with Korean ginseng, the content of ginsenoside was significantly increased. As shown in FIG. 1, the stem A of Korean ginseng cultivated using the fertilizer of the present invention is shorter and thicker than the stem B of Korean ginseng cultivated using the conventional comparative fertilizer 1. Therefore, according to the present invention, Korean ginseng having high quality could be harvested.

[Example 3 (Manufacturing method of sea urchin plant silicon water extract 1)]
Korean ginseng obtained by the cultivation system or cultivation method of Example 1 above was dried at 90 ° C. for 10 hours, 60 g of the dried product (water content 12-14%), 100 g of sodium silicate decahydrate crystals, And 3 L of water was put into a pressure cooker and heated at 85 ° C. to 125 ° C. for about 6 hours to obtain a dark brown ginseng silicon water extract (2970 g, ginseng concentration 2%). In addition, about 100 to 120 g of solid components are precipitated in the Korean ginseng silicon water extract. It was found that when sodium silicate decahydrate aqueous solution was used, the extraction speed was faster than when extracting with water. The Korean ginseng obtained by the same cultivation system or cultivation method as in Example 1 was extracted with only 3 L of water except that 100 g of sodium silicate decahydrate crystals were not added. Didn't go well. Therefore, it was found that it is important to use silicon water.

[Example 4 (Manufacturing method of sea urchin plant silicon water extract 2)]
The Korean ginseng obtained by the cultivation system or cultivation method of Examples 1 and 2 was freeze-dried, 60 g of the dried product was placed in a pressure cooker, and sodium silicate decahydrate used in Examples 1 and 2 above. 3 L of an aqueous solution (10000 ppm) was added and heated at 85 ° C. to 125 ° C. for about 6 hours to obtain a dark brown extract (2970 g). It was found that when sodium silicate decahydrate aqueous solution was used, the extraction speed was faster than when extracting with water.

[Example 5 (Manufacturing method of sea urchin plant silicon water extract 3)]
The Korean ginseng obtained by the cultivation system or cultivation method of Examples 1 and 2 was freeze-dried, 60 g of the dried product, 100 g of sodium silicate decahydrate crystals, and 3 L of water were placed in a pressure cooker at 85 ° C. Heating at ˜125 ° C. for about 6 hours gave a dark brown extract (2970 g). About 100 to 120 g of solid components are precipitated in the extract. It was found that when sodium silicate decahydrate aqueous solution was used, the extraction speed was faster than when extracting with water.

1. Pipe (FRP or iron), 2. Support pipe, 3. 3. Nonwoven fabric, 4. PE film tunnel, Cultivation bed, 6. 6. Infusion hose, Seedlings, 8. Black PE film, 9. 9. Straw foam bed Garden floor soil, 11. Rice husks, 12. Black PE film, 13. Drainage, 14. Coaster pipe, 15. Pipe (FRP or iron), 16. Non-woven fabric, 17. Vinyl tunnel, 18. Iron struts, 19. Cultivation bed, 20. Seedlings, 21. Fixing sponge, 22. Bed top plate, 23. Supply pipe, 24. Drainage channel, 25. Injection nozzle (not ultrasonic), 26. Non-woven fabric, 27. LED, 28. Fog spray nozzle, 29. Vinyl cover, 30. Koryojin, 31. Cultivation bed top plate, 32. Cultivation bed, 33. Ultrasonic spray pump, 34. Pipe, 35. Sponge for fixing

Claims (18)

1. It is a cultivation system for araceae plants,
   (1) means for irradiating purple light, and (2) means for irradiating red light, and (2) the intensity of the red light is 3 to 30 with respect to (1) the intensity of purple light 1. The cultivation system, which is a range.
2. (1) The cultivation system according to claim 1, wherein the violet light has a peak in a wavelength range of 380 to 450 nm.
3. (2) The cultivation system according to claim 1 or 2, wherein the red light has a peak in a wavelength range of 620 to 750 nm.
4. The cultivation system according to any one of claims 1 to 3, wherein the intensity of (2) red light is in the range of 3.2 to 10 with respect to (1) intensity of violet light 1.
5. The cultivation system according to any one of claims 1 to 4, wherein the content of ginsenoside in the araceae plant can be increased.
6. The ginsenoside is ginsenoside F1, ginsenoside F2, ginsenoside F5, ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, ginsenoside Rh2 (S), ginsenoside Rh1 The cultivation system according to claim 5, which is at least one kind of ginsenoside selected from the group consisting of R).
7. The cultivation system according to any one of claims 1 to 6, wherein the Araceae plant belongs to the genus Tochibaninjin.
8. The cultivation system according to any one of claims 1 to 7, further comprising means for applying fertilizer.
9. The cultivation system according to any one of claims 1 to 8, further comprising means for ultrasonically spraying the fertilizer.
10. A method for cultivating medicinal medicinal plants, comprising (1) a step of irradiating violet light, and (2) a step of irradiating red light, and (2) the intensity of the red light is (1) The cultivation method as described above, which is in the range of 3 to 30 with respect to the strength 1.
11. (1) The cultivation method according to claim 10, wherein the violet light has a peak in a wavelength range of 380 to 450 nm.
12. (2) The cultivation method according to claim 10 or 11, wherein the red light has a peak in a wavelength range of 620 to 750 nm.
13. The cultivation method according to any one of claims 10 to 12, further comprising a step of applying a fertilizer.
14. The cultivation method according to any one of claims 10 to 13, further comprising a step of ultrasonically spraying the fertilizer.
15. A cornaceae plant composition obtained by the cultivation system according to any one of claims 1 to 9 or the cultivation method according to any one of claims 10 to 14.
16. The arginaceae plant composition according to claim 15, which satisfies at least one of the following conditions A) to C):
    A) The content of ginsenoside F2 contained in the harvest is 0.1 mg or more / live weight 1 g,
    B) The content of ginsenoside Rg2 (S) contained in the harvest is 0.05 mg or more / live weight 1 g,
    C) Ginsenoside Rf content contained in the harvest is 0.05 mg or more / live weight 1 g.
17. A processed product comprising the Araceae plant composition according to claim 15 or 16.
18. An extract of the Argiaceae plant composition according to claim 15 or 16.

Images