Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K10/00—Animal feeding-stuffs
  • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
  • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
  • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING OR TREATMENT THEREOF
  • A23C9/00—Milk preparations; Milk powder or milk powder preparations
  • A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
  • A23C9/123—Fermented milk preparations; Treatment using microorganisms or enzymes using only microorganisms of the genus lactobacteriaceae; Yoghurt
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
  • A61K35/66—Microorganisms or materials therefrom
  • A61K35/74—Bacteria
  • A61K35/741—Probiotics
  • A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
  • A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K10/00—Animal feeding-stuffs
  • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
  • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
  • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
  • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
  • A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
  • A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0012—Galenical forms characterised by the site of application
  • A61K9/0034—Urogenital system, e.g. vagina, uterus, cervix, penis, scrotum, urethra, bladder; Personal lubricants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/0087—Galenical forms not covered by A61K9/02 - A61K9/7023
  • A61K9/0095—Drinks; Beverages; Syrups; Compositions for reconstitution thereof, e.g. powders or tablets to be dispersed in a glass of water; Veterinary drenches
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/02—Suppositories; Bougies; Bases therefor; Ovules
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
  • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
  • A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/02—Separating microorganisms from their culture media
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
  • C12N1/20—Bacteria; Culture media therefor
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
  • A23V2400/00—Lactic or propionic acid bacteria
  • A23V2400/11—Lactobacillus
  • A23V2400/175—Rhamnosus
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
  • A61K2035/11—Medicinal preparations comprising living procariotic cells
  • A61K2035/115—Probiotics
• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
  • C12R2001/00—Microorganisms ; Processes using microorganisms
  • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
  • C12R2001/225—Lactobacillus

Definitions

• the present invention relates to a novel lactic acid Lactobacillus rhamnosus bacterium suitable for treatment of e.g. bacterial vaginosis.
• Bacterial vaginosis is one of the most common disorders in women of reproductive age. According to the US Centers for Disease Control and Prevention (CDC), risk factors for BV include douching and having new or multiple sex partners. BV is caused by an imbalance of naturally occurring bacterial microbiota and can be microbiologically characterized by replacement of the lac- tobacilli-predominant vaginal microbiota by potential pathogenic vaginal bacteria. The change from a healthy, H 2 0 2 and lactic acid producing lactobacilli-dominated microbiota to a complex mul- tispecies microbiota can occur relatively quickly and result in BV.
• Bacterial vaginosis (BV) is accompanied by a rise in pH (rise from normal/healthy pH of approximately 4 to a pH above 4.5) and a microbial dysbiosis in which the usually dominant Lactobacillus vaginal microbiota is overwhelmed by an overgrowth of predominantly anaerobic organisms.
• EP2509610B1 HSO Health Care, Vienna - filed 13.09.201 1 and published/granted in 2013 provides a summary of herein relevant background prior art.
• Lactobacillus species which play an essential role in protecting women from urogenital infection.
• Lactobacilli have the ability to adhere to vaginal epithelia, to inhibit the adhesion and growth of pathogens, deplete nutrients otherwise available to pathogens, and modulate the host immune response and microenvironment.
• Lactobacilli metabolize the glycogen contained in the cells of the vaginal vault, forming lactic acid as the final product.
• pH-values of around 4.0 are reached, a level at which many pathogens cannot flourish.
• vaginal cap- sules for vaginal administration When administrated to a woman in the form of a pharmaceutical composition (e.g. vaginal cap- sules for vaginal administration) - the protective effect of Lactobacilli against potential pathogens in the vagina is generated through the metabolic activity of the Lactobacilli.
• the bacteria consume glycogen and other sources of glucose and produce lactic acid.
• the low pH generated in this manner is harmful to the less desirable bacteria and fungi and thus protects the vaginal mucosa against infections. Because vaginal infection is an important mechanism of disease responsible for preterm birth, maintaining the natural, healthy balance of the Lactobacillus microbiota in the vagina is particularly important during pregnancy.
• a deficiency in Lactobacilli can upset the microbial balance in the vagina, frequently resulting in the syndrome of bacterial vaginosis, which may be associated with a quantitative and qualitative shift from normally occurring Lactobacilli to a mixed microbiota dominated by anaerobic bacteria.
• bacterial vaginosis may be characterized by a complete loss of Lactobacilli and a concomitant increase in Gram-variable and Gram-negative rods, primary among them Gardnerella vaginalis as well as Bacteroides, Prevotella, and Mobi- luncus species.
• loss of vaginal Lactobacilli also leaves nonpregnant women susceptible to infection which may result in endometritis or even pelvic inflammatory disease.
• vaginal atrophy Vaginal dryness, burning, itching and dyspareunia are frequent com- plaints along with dysuria, urinary frequency and recurrent infections.
• the genitourinary atrophy following menopause is associated with a decline in estrogen secretion accompanied by depletion of Lactobacilli and increased colonization by pathogenic microorganisms associated with bacterial vaginosis and urinary tract infections.
• vaginal estriol therapy reduces Escherichia coli colonization and increases the numbers of Lactobacilli, with the result that the in- cidence of recurrent urinary and genital tract infections drops significantly.
• Lactobacilli topical use of Lactobacilli is a safe and promising treatment for the prevention of vaginosis and recurrent urinary tract infections.
• vaginal supplementation is a long-standing, widely accepted practice for Lactobacilli substitution
• oral administration of a Lactobacillus preparation represents a new concept for the restitution of a normal vaginal microbiota.
• Relatively recent results indicate that the probiotic strains L. rhamnosus GR-1 (ATCC 55826) and L. reuteri RC-14 (ATCC 55845) can be taken orally on a daily basis for two months without any side effects.
• EP2509610B1 relates to a use of a combination of specific deposited strains of L. crispatus, L. rhamnosus, L. jensenii and/or L. gasseri for treatment of bacterial vaginosis (BV).
• table 11 on page 15 is shown that e.g. deposited L. rhamnosus (DS 22560) has a relatively good capability to lower pH.
• the problem to be solved by the present invention is to provide a novel Lactobacillus rhamnosus strain with good properties in particular in relation to the treatment of vaginal and female genital and urogenital infection and/or urinary tract infections caused by Lactobacillus deficiency (such as e.g. bacterial vaginosis).
• RAPD Random Amplified Polymorphic DNA
• BPL005 had the highest capacity of the vagina isolated strains to reduce pH levels
• Genome sequencing of the whole genome of strain BPL005 showed that it had a unique contig of 3.023 Mb - i.e. the genome sequence was not a prior art described sequence.
• Bacterial vaginosis is accompanied by a rise in pH (rise from normal/healthy pH of approximately 4 to a pH above 4.5) and a mi- crobial dysbiosis in which the usually dominant Lactobacillus vaginal microbiota is overwhelmed by an overgrowth of predominantly anaerobic organisms.
• strain BPL005 had similar pH-reducing levels than the best probiotics tested of herein relevant commercial gynaecological probiotic products.
• results of working Example 3 herein showed good efficiency of strain BPL005 in the production process at lab-scale (volume 1 L) and data of the made lyophilized powder showed that the BPL005 strain has obtained a good stability in a 15 months period.
• a first aspect of the invention relates to a lactic acid L rhamnosus bacterium with the registration number CECT 8800 (herein also termed BPL005) or a mutant thereof, wherein the mutant strain is obtained by using the deposited strain as starting material, and wherein the mutant has retained or further improved the capacity to reduce pH levels of CECT 8800.
• a second aspect of the invention relates to a dietetic or a pharmaceutical composition comprising the bacterium of the first aspect and/or any herein described embodiment thereof.
• the term "dietetic" relates according to the art to diet or the regulation of food or feed intake.
• a dietetic composition is a composition that is suitable for being added to a food product for a human or a feed product for an animal.
• a third aspect of the invention relates to a dietetic or pharmaceutical composition according to the second aspect and/or any herein described embodiment thereof, for use in the treatment of a disorder in a woman, wherein the disorder is imbalance of naturally occurring vaginal bacterial micro- biota, vaginal infection, female genital infection, urogenital infection or urinary tract infection.
• a fourth aspect of the invention relates to a dairy product, a drink product, a food product for hu- man nutrition and/or a feed product for animal nutrition characterized in that the product contains a dietetic composition according to the second aspect and/or any herein described embodiment thereof.
• the first aspect of the invention relates to the herein described novel strain or "a mutant thereof ".
• mutant thereof of the first aspect relates to mutant strains obtained by using the deposited strain as starting material.
• the invention relates to a method for obtaining a mutant strain of lactic acid L. rhamnosus bacterium with the registration number CECT 8800 comprising the following steps:
• step (ii) analyzing the in step (i) made mutants and identify a mutant strain that has retained or further improved the capacity of CECT 8800 to reduce pH levels;
• the first aspect relates to a lactic acid L. rhamnosus bacterium with the registration number CECT 8800 (herein also termed BPL005).
• This article discloses the complete genome sequence of the lactic acid L. rhamnosus bacterium with the registration number CECT 8800 of the present invention.
• a dietetic or a pharmaceutical composition - second aspect of the invention is a dietetic or a pharmaceutical composition - second aspect of the invention.
• a second aspect of the invention relates to a dietetic or a pharmaceutical composition
• a dietetic or a pharmaceutical composition comprising the bacterium of the first aspect and/or any herein described embodiment thereof.
• the dietetic composition generally comprises dietetic or nutritional acceptable adjuvants and/or excipients.
• the pharmaceutical composition generally comprises pharmaceutical acceptable adjuvants and/or excipients.
• such composition comprises the bacteria in a concentrated form including frozen, dried or freeze-dried concentrates typically having a concentration of viable cells, which is in the range of 10 4 to 10 14 cfu (colony forming units) per gram of the composition. It may be preferred that the range is in the range of 10 6 to 10 14 cfu (colony forming units) per gram of the composition or is in the range of 10 8 to 10 14 cfu (colony forming units) per gram of the composition. A relevant range may also be in the range of 10 e to 10 13 cfu (colony forming units) per gram of the composition. It may be preferred that the composition comprises freeze-dried (alternatively expressed lyophi- lized) bacteria.
• composition may as further components contain cryoprotectants (e.g. maltodextrine) and/or conventional additives including nutrients such as yeast extracts, sugars and vitamins.
• cryoprotectants e.g. maltodextrine
• conventional additives including nutrients such as yeast extracts, sugars and vitamins.
• composition comprises freeze-dried bacteria then is preferably comprises suitable cryoprotectants (e.g. maltodextrine).
• suitable cryoprotectants e.g. maltodextrine
• the dietetic or a pharmaceutical com- position as described herein will in certain embodiments comprise a multiplicity of strains either belonging to the same species or belonging to different species.
• a typical example of such a useful combination of lactic acid bacteria in a composition as described herein is a mixture of a Leu- conostoc sp. and one or more Lactococcus subsp. such as Lc. lactis subsp. lactis, Lc. lactis subsp. cremoris or Lc.s lactis subsp. lactis biovar. diacetylactis.
• L. crispatus L. rhamnosus
• Lactobacillus jensenii L. gasseri
• compositions according to the inventions suitable for oral intake or topical vaginal administration may be preferred.
• probiotic bacteria for female use can be administered orally in the form of capsules, or (filled in sachets) suspended in a drink, or in the form of fermented milk (yogurt). When administered orally, they are expected to survive passage through the stomach and duodenum (displaying a certain stability towards acid and bile) and temporarily colonize the gut. From there, small numbers of bacteria will ascend to the vagina and (again temporarily) colonize the vaginal mucosa.
• Probiotic bacteria can also be used conventionally as vaginal capsules or suppositories and directly applied to the vagina.
• a composition according to the invention may be administered in the form of suppositories, vaginal capsules for vaginal admin- istration or as coated capsules, tablets, sachets, pills, pearls, vials for oral intake as well as yogurt, yogurt drinks, fermented milk, juices, and other fermented drinks and foods.
• a third aspect of the invention relates to a dietetic or pharmaceutical compo- sition according to the second aspect and/or any herein described embodiment thereof, for use in the treatment of a disorder in a woman, wherein the disorder is imbalance of naturally occurring vaginal bacterial microbiota, vaginal infection, female genital infection, urogenital infection or urinary tract infection.
• a dietetic or pharmaceutical composition as described herein will many times be used in order to restore and/or maintain a healthy vaginal bacterial microbiota.
• the third aspect may alternatively be formulated as relating to a method for treatment of a disor- der in a women, wherein the disorder imbalance of naturally occurring vaginal bacterial microbiota, vaginal infection, female genital infection, urogenital infection or urinary tract infection, in a human woman comprising administering a relevant amount of a dietetic or pharmaceutical composition according to the second aspect and/or any herein described embodiment thereof to the human woman.
• a herein relevant disorder is vaginosis, vaginitis, chronic bacterial vaginosis, chronic yeast infection, chronic urinary tract infection in menopause, atrophic vaginitis, vaginosis or bacterial vaginosis.
• the disorder in a woman is bacterial vaginosis (BV).
• compositions according to the invention are particularly suitable for use in the treatment or prevention of asymptomatic and recurrent bacterial vaginosis in pregnancy or preterm delivery caused by bacterial vaginosis.
• Dairy product, a drink product, a food product etc - fourth aspect of the invention Dairy product, a drink product, a food product etc - fourth aspect of the invention
• a fourth aspect of the invention relates to a dairy product, a drink product, a food product for human nutrition and/or a feed product for animal nutrition characterized in that the product contains a dietetic composition according to the second aspect and/or any herein de- scribed embodiment thereof.
• Dairy products of the present invention can consist of milk, yogurt, cheese, homogenized products (based on milk, cheese, fruit), fermented or non-fermented milk (including powdered milk, non- lactose containing milk, milk shakes) containing probiotics.
• Therapeutic cheese can be obtained by the addition of suitable probiotic microorganisms in a concentrated dried form in a certain processing phase of the cheese in order to guarantee the supply of the dose of the microorganisms necessary for the organism.
• the drinks can be instantaneous drinks or water containing the compositions according to the present invention. Said integrators, dairy and food products are suitable for use in the treatment the same disorders in a women as discussed above - i.e.
• An embodiment of the invention relates to a method of manufacturing a fermented food or fer- mented feed product comprising adding a lactic acid Lactobacillus rhamnosus bacterium of first aspect to a food or feed product starting material and keeping the thus inoculated starting material under conditions where the lactic acid bacterium is metabolically active.
• Useful food product starting materials include any material which is conventionally subjected to a lactic acid bacterial fermentation step such as milk, vegetable materials, meat products, fruit juices, must, doughs, soya and batters.
• the fermented products which are obtained by the method, include as typical examples dairy products such as fermented milk, yogurt, cheese including fresh cheese products or mozzarella, and buttermilk.
• the substrate material is a starting material for an animal feed such as silage, e. g. grass, cereal material, peas, alfalfa or sugar-beet leaf, where bacterial cultures are inoculated in the feed crop to be ensiled in order to obtain a preservation hereof, or in protein rich animal waste products such as slaughtering offal and fish offal, also with the aims of preserving this offal for animal feeding purposes.
• silage e. g. grass, cereal material, peas, alfalfa or sugar-beet leaf
• bacterial cultures are inoculated in the feed crop to be ensiled in order to obtain a preservation hereof, or in protein rich animal waste products such as slaughtering offal and fish offal, also with the aims of preserving this offal for animal feeding purposes.
• Swabs were spread in MRS agar plates and incubated anaerobically at 37°C. Gram-positive, non- sporulated rods were selected as presumptive lactobacilli. Pure cultures were long-term stored (- 20°C) in glycerol for further analysis.
• RAPD agarose Random Amplified Polymorphic DNA
• each strain was cultured anaerobically at 37°C in 1 L fermentors (Biobundle; Applikon), in the industrial medium optimized in Biopolis for lactobacilli.
• 1 L fermentors Biobundle; Applikon
• two conditions were assayed: with and without pH control. In both cases, levels of pH were monitored during the assay.
• Samples were collected throughout the assay.
• 0.8 mL of cell-free supernatant was mixed by vortexing with 0.2 mL of a mixture containing 5% meta-phosphoric acid, copper sulfate (1.56 mg/mL) and 50 mM 4- methyl valeric acid as an internal standard. Samples were then filtered by 0.45 pm pore size and diluted 1/2 and 1/10.
• MIC Minimum Inhibitory Concentration
• Raw sequences obtained from the whole genome sequencing have been assembled constructing a scaffolding to be used for genome annotation.
• tRNAs, rRNA s and open Reading Frames (ORFs) will be predicted.
• ORFs functionality will be annotated by comparing each ORF against a database containing all sequences from the bacteria kingdom. Once gene functionality associated to each ORF, virulence and antibiotic resistance genes will be searched and listed.
• agarose RAPD profiles were performed in order to reject duplicate isolates.
• the fourteen isolates were grouped in six different strains. Strains were identified by 16S rRNA sequencing (Table 1 ) and Lactobacillus strains were selected for further studies (Table 2). In addition, collection strains L. crispatus CECT 4840 and L. iners DSM 13335 were included in the study.
• Table 1 Groups obtained by RAPD profile and 16S rRNA sequencing.
• strain L. rhamnosus BPL005 and L. casei BPL013 were selected for further studies. 4. Identification of the organic acid profile
• Tables 4-5 show the production of organic acids.
• samples were recovered at the end of the fermentation.
• highest levels were obtained for lactic acid, being the highest concentrations 22.53 g/L in the case of BPL013 and 19.90 g/L for BPL005 strain, in fermentations with controlled pH.
• the rapid production of lactic acid in the case of BPL005 strain is in agreement with its high capacity to reduce pH in a short time.
• the production of lactic acid was followed by a decrease in pH in both strains, being more remarkable in the case of strain BPL005.
• strain BPL005 was the best producer in all cases. It has been reported that these organic acids could inhibit pathogen bacteria and yeasts (Juarez ef al. Urogenital pathogen inhibition and compatibility between vaginal Lactobacillus strains to be considered as probiotic candidates. 2011 . European Journal of Obstetrics & Gynecology and Reproductive Biology. 159: 399-406) In the case of lactic acid, levels obtained were higher than oth- er previously described in above cited Juarez ef al. reference.
• Lactic acid 3.172 ⁇ 0.066 12.168 ⁇ 0.223 15.635 ⁇ 0.158 19.731 ⁇ 0.232 Acetic acid 0.105 ⁇ 0.050 0.123 ⁇ 0.058 0.143 ⁇ 0.058 0.145 ⁇ 0.049 Propionic acid 0.740 ⁇ 0.029 0.155 ⁇ 0.029 0.210 ⁇ 0.026 0.134 ⁇ 0.013
• Lactic acid 0,479 ⁇ 0,090 1 ,387 ⁇ 0,096 10,093 ⁇ 0,088 22,814 ⁇ 0,397
• ND Not detected. 5. Resistance to commercial products assays.
• strain BPL005 The whole genome of strain BPL005 has been sequenced. Whole genome extraction has been performed; identification of strain corroborated and sequencing has been applied. The assembly rendered a unique contig of 3.023 Mb. There were not sequences predicted as plasmids.
• RAPD Random Amplified Polymorphic DNA
• BPL005 had the highest capacity of the vagina isolated strains to reduce pH levels
• Genome sequencing of the whole genome of strain BPL005 showed that it had a unique contig of 3.023 Mb - i.e. the genome sequence was not a prior art described sequence.
• Probiotic strains were isolated from commercial products and stored at -80°C. Identification at species level was confirmed by 16S rDNA sequencing. For each strain, pH reduction assays were performed as explained above. BPL005 strain was included as a control.
• probiotics-based products were analysed. Products tested are summarized in Table 8. For each product, concentration was obtained by counts in MRS plates and functionality of strains was tested. To study the functionality, probiotic strains were isolated, identified by 16S rDNA sequencing and its capacity to decrease pH tested.
• GynOphilus 1 Capsules 4,00E+09 0.34 1 ,36E+09 341 mg 1 FLORGYNAL TAM ⁇
• strain BPL005 had similar pH-reducing levels than the best probiotics tested of herein relevant commercial gynaecological probiotic products.
• EXAMPLE 3 Evaluation of the productivity of Lactobacillus strain BPL005 in laboratory-scale production (volume 1L)
• the lyophilized powder was vacuum-packaged and stored at 5°C ( ⁇ 3°C). Stability of the product was checked by counts in MRS during 15 months.
• the lyophilized powder was vacuum-packaged and stored at 5°C. Stability of the product was checked by counts in MRS. The BPL005 strain has obtained a good stability in a 15 months period.
• pellet was resuspended in carrier (maltodextrine-based) and lyophilised in an industrial lyophilizer.
• carrier maltodextrine-based
• viability was checked on MRS agar plates just after fermentation process and after industrial freeze-drying pro- cess.
• the purity of culture and lyophilised product was checked by electrophoretic RAPD (Random Amplification of Polymorphic DNA) profiles of randomly selected colonies.

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