WO2016002505A1 - Filtre ayant une forme externe optimisée de nervures à l'intérieur du filtre - Google Patents

Filtre ayant une forme externe optimisée de nervures à l'intérieur du filtre Download PDF

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Publication number
WO2016002505A1
WO2016002505A1 PCT/JP2015/067335 JP2015067335W WO2016002505A1 WO 2016002505 A1 WO2016002505 A1 WO 2016002505A1 JP 2015067335 W JP2015067335 W JP 2015067335W WO 2016002505 A1 WO2016002505 A1 WO 2016002505A1
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Prior art keywords
filter
cell separation
separation material
cell
liquid
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PCT/JP2015/067335
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English (en)
Japanese (ja)
Inventor
敬太 山下
尚武 前久保
伸好 梅田
伸彦 佐藤
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株式会社カネカ
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Priority to JP2016531248A priority Critical patent/JP6646575B2/ja
Publication of WO2016002505A1 publication Critical patent/WO2016002505A1/fr

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/02—Blood transfusion apparatus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/14—Dialysis systems; Artificial kidneys; Blood oxygenators ; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis
    • A61M1/16—Dialysis systems; Artificial kidneys; Blood oxygenators ; Reciprocating systems for treatment of body fluids, e.g. single needle systems for hemofiltration or pheresis with membranes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
    • A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
    • A61M1/34—Filtering material out of the blood by passing it through a membrane, i.e. hemofiltration or diafiltration
    • A61M1/3403—Regulation parameters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D29/00—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor
    • B01D29/01—Filters with filtering elements stationary during filtration, e.g. pressure or suction filters, not covered by groups B01D24/00 - B01D27/00; Filtering elements therefor with flat filtering elements

Definitions

  • the present invention relates to a cell separation filter or a cell separation method using the same.
  • An erythrocyte product is a blood product that is used when bleeding and erythrocytes are deficient, or when oxygen is deficient due to reduced function of erythrocytes.
  • the erythrocyte preparation does not require leukocytes that induce side effects such as abnormal immune reactions or graft-versus-host disease (GVHD), and it is necessary to remove leukocytes with a filter.
  • GVHD graft-versus-host disease
  • platelets may be removed in addition to leukocytes.
  • the platelet preparation is a blood preparation used for patients who are bleeding or tend to bleed due to lack of blood coagulation factors.
  • unnecessary cells and components other than platelets are removed by centrifugation, and only the necessary platelet components are collected.
  • hematopoietic stem cell transplantation for the treatment of leukemia and solid cancer has been actively performed, and a method of separating and administering leukocyte groups containing hematopoietic stem cells necessary for treatment has been taken.
  • umbilical cord blood is attracting attention in addition to bone marrow and peripheral blood because of its advantages such as low burden on donors and excellent proliferation ability.
  • menstrual blood it has been suggested that there are abundant stem cells in menstrual blood, and menstrual blood that has been discarded may be used as a valuable source of stem cells.
  • leukocytes For bone marrow and peripheral blood, it is desirable to separate and purify leukocytes after removing unnecessary cells, but umbilical cord blood is also becoming popular for banking for relatives and is stored frozen until use. From the necessity, leukocytes are separated and purified for the purpose of preventing red blood cell hemolysis due to cryopreservation.
  • Patent Document 1 a method of collecting leukocytes using a filter material that captures only white blood cells without capturing red blood cells and platelets (Patent Document 1, Patent Document 2, and Patent Document 3) has been reported as a cell separation method.
  • Patent Document 2 a method of collecting leukocytes using a filter material that captures only white blood cells without capturing red blood cells and platelets
  • an object of the present invention is to provide a cell separation filter with improved cell recovery efficiency. Another object of the present invention is to provide a cell separation method capable of efficiently collecting desired cells using the cell separation filter.
  • the present inventors have intensively studied to solve such problems. As a result, the cell recovery rate is significantly improved by forming a raised portion having a predetermined outer shape on the surface of the liquid inlet side or the liquid outlet side in the cell separation material filled in the cell separation filter, In addition, the present inventors have found that blood components that are not recovered but captured by the cell separation material can be significantly reduced, and the present invention has been completed.
  • the gist of the present invention is as follows.
  • a filter having a container filled with a cell separation material, having a liquid inlet at either the upper part or the lower part of the container, and a liquid outlet at the opposite side,
  • the surface of the filled cell separation material has a raised portion that is raised in at least one direction on the liquid inlet side or the liquid outlet side,
  • the minimum value of the thickness of the cell separation material is 1.20 ⁇ 10 to 1.26 ⁇ 10 mm, and the maximum thickness of the cell separation material is 1.26 ⁇ 10 to 2.10 ⁇ 10 mm [1]
  • the cell-containing liquid is introduced from the liquid inlet of the filter according to any one of the above [1] to [9], and is contacted with the cell separation material filled in the filter to obtain leukocytes and / or simple substances.
  • a first step of capturing the nuclei in the cell separator, and A cell separation method comprising a second step of introducing a collection liquid into the filter and collecting white blood cells and / or mononuclear cells from the cell separation material.
  • the first step including a step of introducing a physiological saline or a buffer solution from a liquid inlet of the filter and bringing the cell separation material into contact with the physiological saline or the buffer solution, [10] or [11].
  • the cells in the blood can be efficiently recovered by using the filter of the present invention.
  • FIG. 2 is a top view of the filter shown in FIG. 1 and a vertical cross-sectional view taken along the line AA of the filter.
  • FIG. 3 is an enlarged vertical sectional view of a main part of the filter shown in FIG. 2. It is the schematic which shows the measurement location at the time of measuring the external shape of a protruding part. It is the schematic which shows an example of the pressing member with a nozzle of a filter. It is a longitudinal cross-sectional view of the cell separation material with which the filter shown in FIG. 2 was filled.
  • 1 is a schematic diagram illustrating a structure of a filter used in Example 1.
  • FIG. 1 is a schematic diagram illustrating a structure of a filter used in Example 1.
  • FIG. 3 is a longitudinal sectional view showing an example of an outer shape of a cell separation material 11 filled in a filter used in Example 1.
  • FIG. 1 is a schematic diagram of a circuit of a cell separation device used in Example 1.
  • FIG. It is the figure at the time of putting the cell separation material taken out from the filter after the cell collection
  • the filter of the present invention is a filter having a container filled with a cell separation material, having a liquid inlet at either the upper part or the lower part of the container and a liquid outlet at the opposite side.
  • the surface of the filled cell separation material has a raised portion that is raised in at least one direction on the liquid inlet side or the liquid outlet side,
  • Cell separation material The form of the cell separation material used in the present invention is not particularly limited, and examples thereof include a porous body having a communicating pore structure, a fiber assembly, and a fabric. Preferably it is comprised with a fiber, More preferably, it is a nonwoven fabric.
  • the material for the cell separation material examples include polyolefins such as polypropylene, polyethylene, high density polyethylene and low density polyethylene, polyester, vinyl chloride, polyvinyl alcohol, vinylidene chloride, rayon, vinylon, polystyrene, acrylic (polymethyl methacrylate, poly Hydroxyethyl methacrylate, polyacrylonitrile, polyacrylic acid, polyacrylate, etc.), nylon, polyurethane, polyimide, aramid, polyamide, cupra, polyparaphenylene terephthalamide, carbon, phenol, tetron, pulp, hemp, cellulose, kenaf, chitin, Examples include chitosan, glass, and cotton.
  • polyolefins such as polypropylene, polyethylene, high density polyethylene and low density polyethylene, polyester, vinyl chloride, polyvinyl alcohol, vinylidene chloride, rayon, vinylon, polystyrene, acrylic (polymethyl methacrylate, poly Hydroxyethyl
  • the cell separation material may be composed of a single material among these materials, or may be composed of a composite material obtained by combining a plurality of materials.
  • the average fiber diameter of the cell separation material used in the present invention is not particularly limited as long as it is appropriately selected according to the type of target cells.
  • the cell separation material may be subjected to a hydrophilic treatment.
  • hydrophilizing treatment By hydrophilizing treatment, non-specific capture of cells other than the desired necessary cells can be suppressed, and cell-containing liquid can be passed through the cell separation material without bias to improve performance and improve efficiency of collecting necessary cells. Can be granted.
  • Hydrophilic treatment methods include water-soluble polyhydric alcohols, polymers having hydroxyl groups, cationic groups or anionic groups, or copolymers thereof (for example, hydroxyethyl methacrylate, dimethylaminoethyl methacrylate, or copolymers thereof).
  • Adsorption method water-soluble polymer (polyethylene glycol, polyvinyl pyrrolidone, polyvinyl alcohol, etc.) adsorption method, hydrophilic polymer fixed to hydrophobic membrane (for example, hydrophilic monomer is chemically bonded to the surface) Method), electron beam irradiation method, method of cross-linking insolubilization of hydrophilic polymer by irradiating radiation to cell separation filter in water-containing state, method of insolubilization and immobilization of hydrophilic polymer by heat treatment in dry state , The method of sulfonating the surface of hydrophobic membrane, hydrophilic polymer and sparse A method of forming a membrane from a mixture with a conductive polymer dope, a method of imparting hydrophilic groups to the membrane surface by treatment with an aqueous alkaline solution (NaOH, KOH, etc.), a soaked hydrophobic porous membrane in alcohol, and then treating with a water-soluble polymer aqueous solution
  • hydrophilic polymer examples include polyvinyl pyrrolidone, polyvinyl alcohol, polyethylene glycol, ethylene-vinyl alcohol copolymer, polyhydroxyethyl methacrylate, polysaccharide (cellulose, chitin, chitosan, etc.), water-soluble polyhydric alcohol, and the like.
  • hydrophobic polymer examples include polystyrene, polyvinyl chloride, polyolefin (polyethylene, polypropylene, etc.), acrylic, urethane, vinylon, nylon, polyester, and the like.
  • cell adhesion proteins and antibodies against specific antigens expressed on the target stem cells are immobilized on the cell separation material.
  • cell adhesion proteins include fibronectin, laminin, vitronectin, collagen and the like.
  • antibodies include, but are not limited to, CD73, CD90, CD105, CD166, CD140a, CD271, and the like.
  • immobilization methods include general protein immobilization methods such as cyanogen bromide activation method, acid azide derivative method, condensation reagent method, diazo method, alkylation method, and crosslinking method. It can be used arbitrarily.
  • the container filled with the cell separating material has a liquid inlet at either the upper part or the lower part and a liquid outlet at the opposite side.
  • the liquid introduction port refers to a port for introducing a liquid containing a target cell (also referred to as a cell-containing liquid) into the container from the outside of the container.
  • the liquid outlet port is a port provided on the opposite side of the liquid inlet port with respect to the vertical direction, and is mainly used to discharge the liquid that has passed through the cell separation material during cell separation operation to the outside of the container.
  • the shape of the container may be any shape such as a sphere, a container, a cassette, a bag, and a tube as long as the cell separating material can be incorporated therein.
  • Preferable specific examples include, for example, a cylindrical container having a capacity of about 0.1 to 400 mL and a diameter of about 0.1 to 15 cm, a square or a rectangle having a length of about 0.1 to 20 cm, and a thickness of 0. Examples thereof include a square columnar container of about 1 to 5 cm.
  • the presser with nozzle that can cover the cylindrical main body 3 and the openings at the top and bottom thereof.
  • the members 4 and 5 and the caps 6 and 7 for fixing the main body 3 and the pressing member with nozzle 4 and 5 are configured.
  • the holding members with nozzles 4 and 5 are respectively provided with a liquid inlet 9 for introducing liquid into the container 2 and a liquid outlet 10 for discharging liquid from the container 2.
  • the liquid inlet 9 and the liquid outlet 10 are constituted by nozzles in order to easily connect a tube for feeding a liquid.
  • the shape and size of the nozzle are not particularly limited.
  • the holding members with nozzles 4 and 5 have a stopper shape, and are fixed in contact with the inner surface of the main body 3 by being pushed into the lumen of the cylindrical main body 3.
  • a seal 8 is provided on the contact surface between the presser members 4 and 5 with the nozzle and the main body 3.
  • the seal 8 ensures the airtightness between the presser members 4 and 5 with the nozzle and the main body 3 and prevents the entry of microorganisms and the like from the outside.
  • a resin packing may be provided around a groove provided on the surface of the presser members 4 and 5 with nozzles. There is no particular limitation on the configuration.
  • the presser members 4 and 5 with nozzles may be directly fixed to the main body 3 (not shown).
  • the pressing member with the nozzle and the main body can be fixed by, for example, providing a screw on the surface where the pressing member with the nozzle and the main body come into contact.
  • the cell separation material 11 is stacked and filled.
  • the locations for capturing cells are dispersed, clogging is suppressed, and separation and collection of cells from the filter are also possible. Can be done efficiently. Note that a portion in which cell separation materials having the same fiber diameter are successively laminated is treated as one layer regardless of the number of laminated cell separation materials.
  • the container 2 may be provided with a cleaning liquid inlet (not shown) for cleaning non-adherent cells remaining in the cell separation material 11 independently on the liquid inlet 9 side, or the liquid outlet 10.
  • a cell recovery solution inlet for recovering cells captured by the cell separation material independently for flowing the cell recovery solution in the direction opposite to the flow of the cell-containing solution and the washing solution) may be provided. (Not shown).
  • the container 2 can be manufactured using any structural material.
  • the structural material include non-reactive polymers, biocompatible metals, alloys, and glass.
  • Non-reactive polymers include acrylonitrile polymers such as acrylonitrile butadiene styrene terpolymers; polytetrafluoroethylene, polychlorotrifluoroethylene, copolymers of tetrafluoroethylene and hexafluoropropylene, halogenated polymers such as polyvinyl chloride; polyamides and polyimides , Polysulfone, polycarbonate, polyethylene, polypropylene, polyvinyl chloride acrylic copolymer, polycarbonate acrylonitrile butadiene styrene, polystyrene, polymethylpentene and the like.
  • Metal materials (biocompatible metals, alloys) useful as container materials include stainless steel, titanium, platinum, tantalum, gold, and alloys thereof, as well as gold-plated alloy iron, platinum-plated alloy iron, cobalt chromium alloy, and nitride.
  • Examples thereof include titanium-coated stainless steel.
  • Particularly preferred is a material having sterilization resistance, and specific examples include polypropylene, polyvinyl chloride, polyethylene, polyimide, polycarbonate, polysulfone, and polymethylpentene.
  • the filter of the present invention has a raised portion that is raised on at least one direction on the liquid inlet side or the liquid outlet side on the surface of the cell separation material filled in the container.
  • the raised portion is a raised portion (12 a, 12 a ′, 12 a ′′, 12 b, 12 b ′ on the surface of the cell separation material 11. , 12b ′′). More specifically, as shown in FIGS.
  • the recessed part between the said protruding parts means the recessed part between protruding parts.
  • the filter 1 shown in FIG. 2B has three raised portions 12a, 12a ′, 12a ′′ on the surface of the cell separation material 11 on the liquid inlet 9 side, and two recessed portions 15a, 15a ′ therebetween.
  • the surface of the cell separation material 11 on the liquid outlet 10 side has three raised portions 12b, 12b ′, 12b ′′, and two concave portions 15b, 15b ′ therebetween.
  • the concave portions 15a and 15b between the raised portions are both valley shapes that are recessed in a curved shape between two substantially mountain-shaped raised portions.
  • the concave portion refers to a concave portion in a state where the concave portion can directly contact the cell-containing liquid introduced into the filter. Therefore, as will be described later, by providing the protrusions 16 on the holding members 4 and 5 with the nozzles, the protrusions and the surface of the cell separation material are formed in the recessed portions generated by pressing the surface of the cell separation material 11. Does not correspond to the recess between the raised portions.
  • the distal end surface of the holding member with nozzles 4 and 5 inserted into the lumen of the main body 3 of the container 2 is shown in FIG.
  • the surface of the cell separation material 11 filled in the container 2 can be pressed.
  • the said protrusion parts 16a and 16b protrude to the lumen
  • the part can be formed.
  • the shape of the tip surfaces of the protrusions 16a and 16b is not particularly limited, and examples thereof include a linear shape, a rod shape, a polygonal shape, an elliptical shape, a circular shape, or a shape obtained by combining two or more of these shapes.
  • a linear shape a rod shape, a polygonal shape, an elliptical shape, a circular shape, or a shape obtained by combining two or more of these shapes.
  • the surface of the cell separation material is pressed with the protrusions, distortion occurs between the protrusions, and the recesses between the protrusions are easily formed. For example, as shown in FIG.
  • the periphery of the cell separation material 11 is pressed into a round shape with a substantially circular protrusion 16a, and further, a rod-shaped protrusion 16b from the periphery to the center at intervals of approximately 120 °.
  • the pressure is pressed, distortion occurs on the surface of the cell separation material 11 that is not pressed, and a plurality of ridges and the ridges of these ridges are formed as shown in FIGS. 2 (b), 3 (a), and 3 (b).
  • a plurality of substantially valley-shaped recesses are formed therebetween.
  • the raised portions 12a and 12a '' are large raised portions formed on the surface of the cell separation material 11 surrounded by the substantially circular protruding portions 16a and 16b in three directions, and the protruding portions 12a ′. Is a small raised portion near the center in the downward direction of the liquid inlet 9. Therefore, four raised portions are formed on the surface of the cell separating material 11 on the liquid inlet 9 side. Moreover, since the recessed part 15a arises between the said large protruding part 12a and the small protruding part 12a '(it is the same also between the said protruding parts 12a''and12a'), the cell separation material 11 by the side of the liquid inlet 9 is produced. There are a total of three recesses on the surface of
  • the area of the front end surface of the protrusion is preferably 30% or less of the surface area of the cell separation material from the viewpoint of facilitating formation of a raised portion, particularly a recessed portion between raised portions.
  • the area of the tip surface of the protrusion may be calculated by measuring using a ruler or the like, and the vertical length (y) and horizontal length (x) in the cross-sectional area of the tip surface are measured.
  • the origin (0, 0) for determining the approximate area may be calculated by measuring a plurality of points other than the origin and performing integral calculation using Excel.
  • the tip surface of the protrusion may be adjusted so that the heights of all the protrusions coincide with each other, or the tip surface may be adjusted to change the height of the protrusion. You may adjust so that there may be a three-dimensional level
  • the expression (1) is an expression showing the outer shape of a substantially parabolic ridge on the upper surface in a longitudinal section parallel to the vertical direction of the cell separation material.
  • said Formula (2) is a type
  • the position of the longitudinal section for confirming the outer shape of the raised portion is not particularly limited as long as a substantially parabolic section can be confirmed.
  • the height and length of the cross section of the raised portion shown in the longitudinal section are the height y of the raised portion and the length x of the raised portion, respectively.
  • the lowest position of the raised portion is set as the origin (0, 0).
  • the upward height is indicated by “+”
  • the downward height is indicated by “ ⁇ ”.
  • the a is an index indicating the size of the bulge in the bulge that satisfies the formula (1) or the formula (2), and the higher the value of the a, the higher the bulge is in the upward or downward direction. Become. On the other hand, the smaller the value of a, the lower the bulge is in the upward or downward direction. From the viewpoint of maintaining the structure and function of the filter itself and performing ideal fluid flow during cell recovery, the a is preferably 0.030 to 0.120, and 0.036 to 0.115. It is more preferable.
  • the b is preferably from 0.3 to 1.5, and more preferably from 0.4 to 1.4.
  • the origin O1 is first determined, and then the positions of four points (R1, R2, R3, R4) from the origin O1 are set to R1. It is measured as (y1, x1), R2 (y2, x2), R3 (y3, x3), R4 (y4, x4). Then, the numerical formula (1) can be calculated by inputting these numerical values into spreadsheet software and calculating an approximate curve.
  • an origin O2 different from the raised portion 12a is determined, and then four points (R5, R6, R7, R8) from the origin O2. ) Is measured as R5 (-y5, x5), R6 (-y6, x6), R7 (-y7, x7), R8 (-y8, x8). Then, the numerical formula (2) can be calculated by inputting these numerical values into spreadsheet software and calculating an approximate curve.
  • the ratio of a and b (a / b) is adjusted to a range of 0.03 to 0.25.
  • the cell separation material has an ideal shape suitable for cell recovery, and has a shape with a plurality of ridges on the surface. It is conceivable that, when the cell is collected, ideal liquid flow is performed, and as a result, the cell collection efficiency is improved.
  • the a / b is more preferably 0.05 to 0.10, and further preferably 0.06 to 0.093.
  • At least one ridge is provided on at least one surface on the liquid inlet side or the liquid outlet side, and at least one concave portion is provided between the ridges. It is preferable.
  • the raised part and the recessed part between raised parts said by said Formula (c), (d) are the raised part and recessed part between raised parts seen in the longitudinal cross-section of the cell separation material with which the filter was filled.
  • Examples of the cell separation material satisfying the relationship of the formula (c) include a cell separation material having a raised portion on one of the liquid inlet side and the liquid outlet side.
  • a cell separation material having a raised portion on one of the liquid inlet side and the liquid outlet side For example, when the pressing member with nozzle 4 shown in FIG. 5 is used on the liquid inlet side or the liquid outlet side of the filter 1, four raised portions and three recessed portions between the raised portions are generated on the surface of the cell separation material 11. Therefore, the relationship of the formula (c) is satisfied.
  • a cell separation material of another form satisfying the relationship of the formula (c) for example, a case where a nozzle having a different number of protrusions 16b on the holding member 4 with nozzle from the number shown in FIG. 5 is used. Can be mentioned.
  • protrusions 16b when there are two protrusions 16b, there are two oval ridges between the protrusions and one circular ridge at the center, and there is a recess between the oval ridge and the circular ridge. Since there is one, three raised portions and two recessed portions between the raised portions are generated on the surface of the cell separation material. Further, when there are four protrusions 16b, there are four elliptical bulges between the protrusions and a circular bulge at the center, and one recess between the elliptical bulge and the circular bulge. For this reason, five raised portions and four recessed portions between the raised portions are formed on the surface of the cell separation material.
  • Examples of the cell separation material that satisfies the relationship of the formula (d) include cell separation materials that have raised portions on both the liquid inlet side and the liquid outlet side. For example, when the pressing member with nozzle 4 shown in FIG. 5 is used on the liquid inlet side and the liquid outlet side of the filter 1, the raised portions and the recessed portions between the raised portions are formed on the upper and lower surfaces.
  • the cell separation material has eight ridges and six ridges between the raised portions, and satisfies the relationship of the above formula (d).
  • Examples of the cell separation material in another form that satisfies the relationship of the formula (d) include, for example, a case where a nozzle different from the number of the protrusions 16b illustrated in FIG.
  • the pressing member with nozzle 4 having two protrusions 16b when used, six ridges and four recesses between the ridges are formed on the surfaces of the upper and lower cell separation materials, and there are four protrusions 16b. In the case where the pressing member with nozzle 4 is used, 10 raised portions and 8 recessed portions between raised portions are generated on the surface of the cell separating material.
  • the surface of the cell separation material to be filled is generally suppressed in a substantially flat shape with respect to the vertical direction by a lid, a partition wall or the like. Therefore, the raised portions, especially the recessed portions between the raised portions, have a shape that cannot be seen on the surface of the cell separation material filled in the conventional filter.
  • the minimum thickness of the cell separation material is preferably 1.20 ⁇ 10 to 1.26 ⁇ 10 mm. Further, the maximum value of the thickness of the cell separation material is preferably 1.26 ⁇ 10 to 2.10 ⁇ 10 mm, and more preferably 1.55 ⁇ 10 to 2.05 ⁇ 10 mm.
  • the thickness of the cell separating material including the raised portions 12a and 12b is the thickness of the cell separating material including the other raised portions.
  • this thickness X becomes the maximum value of the cell separation material 11.
  • the minimum value of the thickness of the cell separating material 11 is, for example, the thickness Y between the surfaces of the cell separating material 11 in the portion pressed by the protrusion 16a in FIG.
  • the longitudinal section of the cell separation material 11 is observed at various angles with respect to the center of the cell separation material, What is necessary is just to measure the maximum thickness and the minimum thickness.
  • the longitudinal section of the filter is observed by imaging with X-ray CT, and the length of each part of the longitudinal section of the cell separation material 11 is determined by CT data viewer (product name “myVGL2” manufactured by Volume Graphics). .2 ").
  • the sum of the cross-sectional areas of the raised portions is 2.08 ⁇ 10 to 1.09 ⁇ 10 2 mm 2
  • the cross-sectional area other than the raised portions is 5.40 ⁇ 10 2 to 5.61 ⁇ .
  • the cross-sectional area of the cell separation material refers to a vertical cross-sectional area when the cell separation material is cut in parallel to the vertical direction of the container.
  • the cross-sectional area of the raised portion refers to a cross-sectional area when the cell separation material is cut in the vertical direction at a portion including a position where the height of the raised portion is maximum with respect to the upward or downward direction.
  • the cross-sectional area other than the raised portion refers to an area obtained by subtracting the cross-sectional area of the raised portion from the total cross-sectional area measured by cutting the cell separating material in the vertical direction as described above.
  • FIG. 6 shows a longitudinal section of the cell separation material 11 of the filter 1 shown in FIG.
  • the cross-sectional area of the cell separation material is all the cross-sectional areas ( ⁇ + ⁇ + ⁇ ′) shown in the figure.
  • the cross-sectional area of the raised portion there are a cross-sectional area ⁇ on the liquid inlet 9 side and a cross-sectional area ⁇ ′ on the liquid outlet 10 side, and the sum ( ⁇ + ⁇ ′) thereof becomes the cross-sectional area of the raised portion.
  • the remaining cross-sectional area ((beta)) which pulled the cross-sectional area of the protruding part from the cross-sectional area of the said cell separation material becomes cross-sectional area other than a protruding part.
  • the ratio ( ⁇ + ⁇ ′) / ⁇ is more preferably adjusted to a range of 0.23 to 0.42.
  • the cells targeted in the present invention may be white blood cells or mononuclear cells, and are not particularly limited.
  • artificial pluripotent stem cells iPS cells
  • embryonic stem cells ES cells
  • mesenchymal stem cells Adipose-derived mesenchymal cells
  • adipose-derived stromal stem cells pluripotent adult stem cells
  • bone marrow stromal cells hematopoietic stem cells and other multipotent biological stem cells
  • T cells, B cells killer T cells (cytotoxic T cells)
  • Lymphocyte cells such as NK cells, NKT cells, regulatory T cells, macrophages, monocytes, dendritic cells, granulocytes, erythrocytes, platelets, etc., nerve cells, muscle cells, fibroblasts, hepatocytes
  • somatic cells such as cardiomyocytes or cells that have undergone treatment such as gene introduction or differentiation.
  • the filter of the present invention is suitably used for collecting various cells, particularly leukocytes, hematopoietic stem cells and / or mononuclear cells.
  • leukocytes include granulocytes such as neutrophils, eosinophils and basophils in peripheral blood, and mononuclear cells such as monocytes and lymphocytes.
  • a cell-containing liquid is introduced from the liquid inlet of the filter and brought into contact with a cell separation material filled in the filter, whereby leukocytes and / or mononuclear cells are separated from the cell separation material.
  • a first step to be captured by, and Examples include a method including a second step of introducing a collection solution into the filter and collecting white blood cells and / or mononuclear cells from the cell separation material.
  • the cell-containing liquid can be used without particular limitation as long as it is a suspension containing cells containing the leukocytes, hematopoietic stem cells and / or mononuclear cells.
  • biological tissue such as the umbilical cord can be treated with an enzyme.
  • suspensions prepared by pretreatment such as sonication and so on.
  • it may be a suspension obtained by culturing or proliferating cells such as leukocytes exemplified above using a culture solution or a stimulating factor in vitro.
  • the cell-containing liquid when the cell-containing liquid is introduced from the liquid inlet of the filter, the cell-containing liquid is introduced into the filter by pressurizing the cell-containing liquid, By contacting with the cell separation material filled in the filter, leukocytes and / or mononuclear cells are captured by the cell separation material.
  • the degree of pressurization is not particularly limited.
  • the cell separation material a material capable of capturing leukocytes and / or mononuclear cells may be used.
  • blood components other than leukocytes may be removed as unnecessary components or may be collected as necessary, and there is no particular limitation.
  • the collection liquid is introduced into the filter that has captured white blood cells, etc., so that the white blood cells and the like are separated from the filter into the collection liquid, and the collection liquid containing the white blood cells and the like is collected from the liquid inlet.
  • white blood cells and the like can be collected.
  • the recovered liquid is introduced into the filter from the liquid outlet of the filter.
  • the recovered solution is not particularly limited as long as it is a solution that is isotonic with cells, and examples include those that have been used as injection solvents such as physiological saline and Ringer's solution, buffers, and culture media for cell culture. .
  • a medium that can be cultured as it is after passing through the culture step is preferable, and when it is used as it is for the treatment without passing through the culture step, safety such as an isotonic solution that has been used for infusions such as physiological saline is guaranteed. It is preferable to use the recovered liquid.
  • the first step and the second step may be performed at the room temperature or may be performed at a refrigeration temperature.
  • processing the refrigerated cell containing liquid is mentioned.
  • Examples of storage of the cell-containing liquid include storage by a refrigerator set at a refrigeration temperature, storage by a water bath, storage by dry ice, and the like. It is preferable to store in a refrigerator because of its versatility.
  • the refrigeration temperature is preferably 1 ° C or higher and 6 ° C or lower, more preferably 3 ° C or higher and 5 ° C or lower. If the refrigeration temperature is less than 1 ° C, the cells die, and if the storage temperature exceeds 6 ° C, the bacteria may propagate and cause contamination.
  • a physiological saline or buffer solution is introduced from the liquid inlet of the filter, and the cell separation material and the physiological Saline or buffer may be contacted.
  • physiological saline or a buffer solution is introduced from the liquid inlet of the filter and led out from the liquid outlet of the filter, thereby By removing the contaminating components, unnecessary components can be reduced in the collected cells.
  • Example 1 As shown in FIG. 7, 112 cylindrical non-woven fabrics (cell separators 11) made of polybutylene terephthalate are filled in a cylindrical container body 3 made of polycarbonate having a diameter of 52 mm in a stacked state.
  • the structure shown in FIGS. 1, 2 (a) and 2 (b) is obtained by inserting the presser members 4 and 5 shown in FIG. 5 into the upper and lower openings and screwing them with caps 6 and 7 from above.
  • the filter 1 which has was produced.
  • the protrusions 16b of the two upper and lower nozzle pressing members 4, 5 are aligned, and the thickness of the nonwoven fabric pressed by the tip surface of the protrusion 16b is the minimum value of the cell separation material. I did it. In addition, the distance between the tip surfaces of the upper and lower protrusions 16b was adjusted to 12 mm (the minimum value of the thickness of the cell separation material).
  • the raised portions 12a, 12a ′, The raised portions 12b and 12b ′ and the recessed portion 15b were confirmed on the surface of the recessed portion 15a and the liquid outlet port side.
  • the raised portions 12 a ′ and 12 b ′ are both between the protruding portions 16 b and are large raised portions having a substantially elliptical shape when viewed from the upper surface.
  • the raised portions 12 a and 12 b are both of the cell separating material 11. It was near the center and was a small bulge with a substantially circular shape when viewed from above.
  • the position of the longitudinal section to be observed is rotated by about 3 ° from the center of the cell separation material, and the longitudinal section including the raised portion on the upper surface next to the protruding portion 16b is observed.
  • the origin was determined, and the height and length of the raised portions at a plurality of points from the origin were measured. The results are shown in Table 2.
  • the state of the cell separation material filled in the filter 1 was as follows. (Thickness of cell separation material) Maximum thickness of cell separation material in filter (X): 18.46 mm Minimum thickness (Y) of cell separation material in the filter: 12.09 mm Thickness of the cell separation material from the concave portion between the raised portions on the liquid inlet side to the concave portion between the raised portions on the liquid outlet side: 13.96 to 16.92 (mm) (Cross sectional area of cell separation material) Sum of cross-sectional areas of raised portions: 144.93 to 182.46 mm 2 Cross-sectional area other than the raised portion: 545.86 mm 2
  • Example 2 The filter 1 was produced in the same manner as in Example 1 except that the protrusions 16b of the upper and lower two presser members 4 and 5 in the filter 1 were not aligned and fixed by rotating by 60 ° from the center. .
  • Example 3 A filter 1 was produced in the same manner as in Example 1 except that the number of cell separation materials to be laminated was 140. The minimum value of the thickness of the cell separation material was not changed, and the compression was strengthened by changing the number of stacked layers so that the cell separation material was more easily raised.
  • Example 1 except that it was pressed in advance so that the height of the laminated state was 12 mm, and was filled with non-woven fabric (112 sheets) in which the surface of the cell separation material was substantially planar and the height of the raised portions was suppressed. Similarly, filter 1 was produced.
  • a tube 17a is connected to the liquid inlet 9, and the tube 17a is connected to a means 18 for containing a cell suspension and a means 19 for containing a priming physiological saline.
  • the tube 17b and the tube 17c connected to the means (collection bag) 20 for storing the collected liquid that has passed through the filter and the means 21 for collecting the collected liquid collected in the collection bag or the like are connected via the flow path switching means 22a.
  • the tube 17b was connected with a means 18 for containing a cell suspension and a means 19 for containing a priming physiological saline via a flow path switching means 22b.
  • the tube 17c is connected to a means 20 for storing the recovered liquid that has passed through the filter and a means 21 for recovering the recovered liquid collected in a recovery bag or the like via a flow path switching means 22c. Further, a tube 17d is connected to the liquid outlet 10, and a means (waste liquid bag) 23 for containing the cell suspension that has passed through the filter and a means 24 for collecting the recovered liquid are connected via the flow path switching means 22d. Connected.
  • each flow path switching unit was appropriately performed according to the type of liquid passing through the filter 1 and the target unit for liquid transfer.
  • a priming operation is performed by manually passing 150 mL of physiological saline through the filter 1 using a syringe, and the physiological saline that has passed through the filter 1 is passed through the waste liquid bag 23. It was collected.
  • CPD blood preservation solution C solution
  • HES hydroxyethyl starch
  • white blood cells were recovered from the liquid inlet 9 into the recovery bag 20.
  • a blood cell counter (trade name “K-4500”, manufactured by Sysmex Corporation) was used for blood count of blood before treatment and blood count of the solution after recovery, and the leukocyte recovery rate was calculated from each measurement result.
  • Test Example 2 Cell separation materials were taken out from the filters used in Examples 1 and 3 and Comparative Example 1 after white blood cell collection, and placed on white absorbent paper. As a result, as shown in FIG. 10, the cell separation material obtained in Examples 1 and 3 had almost the original color (white) of the cell separation material, whereas the cell obtained in Comparative Example 1 A large amount of blood-derived components remained from the separating material and stained red. Therefore, as in the filter 1 used in Examples 1 and 3, a raised portion that protrudes in at least one direction on the liquid inlet side or the liquid outlet side on the surface of the packed cell separation material. It can be seen that the formation and the outer shape of the cell separation material satisfy a predetermined formula can reduce the recovery loss of blood components and can recover the target cells more efficiently.
  • the cells collected in the present invention can be provided by culturing and proliferating the cells, or can be used without being proliferated, and may be used as therapeutic cells.
  • Specific treatment subjects include ischemic diseases and vascular diseases, but are not limited thereto.
  • Cells to be transplanted into a cartilage injury patient by differentiation induction with a differentiation inducer or the like cells to be transplanted into a bone disease patient, cells to be transplanted into a myocardial disease patient or vascular disease patient, neural tissue
  • the present invention is not limited to these cells.
  • the differentiation inducer those capable of inducing differentiation of the target cells are preferably used, but as the differentiation inducer for cartilage, dexamethasone, TGF ⁇ , insulin, transferrin, ethanolamine, proline, ascorbic acid, pyruvate Salt, selenium, etc .; bone differentiation inducers include dexamethasone, ⁇ -glycerophosphate, vitamin C, ascorbate, etc .; myocardial differentiation inducers include EGF, PDGF, 5-azacytidine Examples of the differentiation inducer to nerve include EGF, bFGF, bHLH and the like; Examples of the differentiation inducer to blood vessel include bFGF, VEGF and the like.
  • the cells collected or grown according to the present invention may be stored frozen. From the point that damage to cells can be reduced, it is preferably cryopreserved using liquid nitrogen. Alternatively, cryopreserved cells can be thawed and transplanted into humans or animals, used for research, or cultured again.
  • the pharmaceutical composition can be produced using the cells collected or grown according to the present invention.
  • a pharmaceutical composition can be produced by mixing the cells with a pharmaceutically acceptable additive.
  • pharmaceutically acceptable additives include coagulants, nutrient sources such as vitamins, and antibiotics.

Abstract

L'invention concerne un filtre ayant un récipient rempli d'un matériau de séparation de cellules. Le filtre comprend un orifice d'introduction d'un fluide, dans une section supérieure ou une section inférieure du récipient, et un orifice d'évacuation du fluide sur son côté opposé. Le filtre possède des nervures sur la surface du matériau de séparation de cellules tel qu'introduit, qui dépassent vers le côté orifice d'introduction du fluide ou vers le côté orifice d'évacuation du fluide. La hauteur (y) et la longueur (x) des nervures satisfont à la formule (1) y = -ax2 + bx et/ou à la formule (2)) y = ax2 - bx. Le rapport (a/b) entre a et b est ajusté à une valeur comprise dans la plage de 0,03 à 0,25. L'objet de la présente invention est de fournir un filtre de séparation de cellules présentant un rendement amélioré de récupération des cellules, et un procédé de séparation de cellules qui utilise le filtre de séparation de cellules, de sorte que des cellules cibles peuvent être efficacement récupérées.
PCT/JP2015/067335 2014-06-30 2015-06-16 Filtre ayant une forme externe optimisée de nervures à l'intérieur du filtre WO2016002505A1 (fr)

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JP2016010394A (ja) * 2014-06-30 2016-01-21 株式会社カネカ フィルター内の隆起部の比率を最適化したフィルター
WO2018194061A1 (fr) * 2017-04-17 2018-10-25 株式会社カネカ Récipient de filtre pour séparer des cellules et dispositif de filtre pour séparer des cellules
WO2020054755A1 (fr) * 2018-09-11 2020-03-19 日産化学株式会社 Dispositif de séparation et procédé de séparation de matériau à séparer l'utilisant
CN114126773A (zh) * 2019-09-17 2022-03-01 株式会社村田制作所 过滤回收装置

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WO2012039400A1 (fr) * 2010-09-21 2012-03-29 旭化成メディカル株式会社 Filtre de traitement de sang et procédé de production de ce filtre
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JP2003274923A (ja) * 2002-03-22 2003-09-30 Terumo Corp 単球捕捉フィルターおよび単球分離培養装置、並びに樹状細胞回収方法
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JP2016010394A (ja) * 2014-06-30 2016-01-21 株式会社カネカ フィルター内の隆起部の比率を最適化したフィルター
WO2018194061A1 (fr) * 2017-04-17 2018-10-25 株式会社カネカ Récipient de filtre pour séparer des cellules et dispositif de filtre pour séparer des cellules
WO2020054755A1 (fr) * 2018-09-11 2020-03-19 日産化学株式会社 Dispositif de séparation et procédé de séparation de matériau à séparer l'utilisant
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CN114126773B (zh) * 2019-09-17 2023-10-03 株式会社村田制作所 过滤回收装置

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