WO2014193162A1 - Procédé de préparation d'un biopesticide au moyen d'une souche de paecilomyces sp. - Google Patents

Procédé de préparation d'un biopesticide au moyen d'une souche de paecilomyces sp. Download PDF

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Publication number
WO2014193162A1
WO2014193162A1 PCT/KR2014/004766 KR2014004766W WO2014193162A1 WO 2014193162 A1 WO2014193162 A1 WO 2014193162A1 KR 2014004766 W KR2014004766 W KR 2014004766W WO 2014193162 A1 WO2014193162 A1 WO 2014193162A1
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WO
WIPO (PCT)
Prior art keywords
weight
parts
composition
oil
spores
Prior art date
Application number
PCT/KR2014/004766
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English (en)
Korean (ko)
Inventor
박호용
손광희
전기
신동하
오현우
조한영
Original Assignee
한국생명공학연구원
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Application filed by 한국생명공학연구원 filed Critical 한국생명공학연구원
Priority to JP2016516445A priority Critical patent/JP2016526043A/ja
Publication of WO2014193162A1 publication Critical patent/WO2014193162A1/fr

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom

Definitions

  • the present invention relates to a method for preparing a pesticide biopesticide using active spores of the strains of the genus Pacylomyces (/ 3 ⁇ 4ec / AM? Yces).
  • Crop protection materials are classified into biopesticides and chemical pesticides, and biopesticides are classified into biological pesticides, including microbiological pesticides and biochemical pesticides, and others that use natural enemies, ships, and parasites.
  • biopesticides using microorganisms, asbestos, etc., are classified as organic materials.
  • the present invention is 5 to 10 parts by weight of the culture medium or active spores thereof, 90 to 98 parts by weight of vegetable oil, emulsifier 2 to 5 parts by weight of the culture medium of the strain of the genus Pacillomyces relative to the total weight of the oil composition
  • the agent composition is provided.
  • the present invention is 5 to 10 parts by weight of the culture medium or active spores of the strain of the genus Paciromomyses (/ 3 ⁇ 4ec / / z? Yces), 30 to 50 parts by weight of corn starch or potato starch,
  • a pest control method comprising the step of treating a plant, soil or seed with a pesticide wetting agent composition comprising 30 to 50 parts by weight of excipients and 30 to 50 parts by weight of dextrin.
  • Figure 5 is a diagram confirming the pesticidal effect of the hydrating agent and emulsifying agent containing the active spores of the Pacillomyces lysinus HY-4 strain.
  • the vegetable oil is preferably at least one selected from the group consisting of corn oil, soybean oil, grape seed oil, canola oil, olive oil, kerosene and animal oil, but is not limited thereto.
  • the emulsifier is preferably at least one selected from the group consisting of Tween 20, Tween 80, Span 80, Span 85, and Triton X-100, but is not limited thereto.
  • the present inventors in order to examine the stability of the active spores for various emulsifiers, adding the active spores of the present invention to soybean oil, suspended by the addition of emulsifiers and then confirmed the germination of the spores, and as a result of measuring the survival rate, The emulsifier It was confirmed that there was no significant effect on the germination of spores until 6 months (see FIG. 2).
  • the present inventors confirmed the stability improvement effect of the active spores of the present invention using various antioxidants, it was confirmed that the active spores with the addition of antioxidants maintained the spore survival rate of 88% or more even after 210 days (see Fig. 4) .
  • the emulsion composition of the present invention facilitates dispersing, mixing and suspending the active spores produced from the strains of the genus Pacillomyces in water and solvents, and can maintain and stabilize the intrinsic pest control activity. It can be usefully used.
  • the present invention is a pest control oil comprising a culture solution of the genus of Pacillomyces strain or 5 to 10 parts by weight of its active spores, 90 to 98 parts by weight of vegetable oil, 2 to 5 parts by weight of an emulsifier based on the total weight of the oil composition
  • the agent composition is provided.
  • the oil-based composition for controlling the sea layer is preferably an improved sea layer control activity and stability, but is not limited thereto.
  • the culture solution or the active spores of the strain of the genus Paciromomyses contains 5 parts by weight or less based on the total weight of the oil composition, so the pest control effect is significantly reduced,
  • Culture medium of the genus strain or active spores thereof is preferably contained 5 to 10 parts by weight based on the total weight of the emulsion composition, but is not limited thereto.
  • composition prepared in step 2) is a known adjuvant as needed It may further include.
  • the adjuvant may include, in particular, stabilizers, antioxidants, thickeners and antifoaming agents, but is not limited thereto.
  • the hydrate, emulsion or oil composition prepared by the method of the present invention facilitates the dispersion, mixing and suspending the active spores produced from the strains of the genus Pacylomyces in water or solvents, and provides unique pest control activity. Since it can maintain and stabilize, it can be usefully used for pest control.
  • the present invention is based on the total weight of the hydrating agent composition of the culture solution of the strains of the genus Paciromomyses (/ 3 ⁇ 4ec / o / 3 ⁇ 4 ces) or its active spores 5 to 10 30 to 50 parts by weight of corn starch or potato starch, 30 to 50 parts by weight of excipients and 30 to 50 parts by weight of dextrin 30 to 50 parts by weight of the pesticide control method comprising the step of treating the plant, soil or seed to the hydration control composition To provide.
  • the present invention is 5 to 10 parts by weight of the culture medium or active spores thereof, 50 to 60 parts by weight of vegetable oil, 30 to 40 parts by weight of water, emulsifiers 2 to 5 parts by weight based on the total weight of the emulsion composition It provides a pest control method comprising the step of treating the pesticide emulsion composition for controlling plants, soil or seeds.
  • the spore survival rate was significantly decreased in the case of the emulsion without an emulsion starting from 120 days after storage, maintaining 5 survival rates after 180 days, while the storage sample used as an emulsion was continuously Spore viability of at least 90% was maintained (FIG. 2).

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  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne un procédé de préparation d'un matériau organique écologique, ou biopesticide, au moyen d'une souche de Paecilomyces sp. Plus particulièrement, une composition de poudre hydrodispersible, de concentré pouvant former une émulsion ou d'agent huileux destinée à la lutte contre les nuisibles, laquelle sont préparés en mélangeant, dans une condition optimale, une spore active produite à partir de la souche de Paecilomyces sp. avec un excipient, un émulsifiant, etc., est facilement dispersée, mise en suspension ou mélangée dans de l'eau ou un solvant organique, conserve un effet unique de prévention des insectes, augmente la stabilité, et peut être ainsi utilisée efficacement pour la lutte contre les nuisibles.
PCT/KR2014/004766 2013-05-31 2014-05-28 Procédé de préparation d'un biopesticide au moyen d'une souche de paecilomyces sp. WO2014193162A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2016516445A JP2016526043A (ja) 2013-05-31 2014-05-28 ペシロマイセス属菌株を用いた生物農薬の製造方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2013-0062878 2013-05-31
KR1020130062878A KR20140141857A (ko) 2013-05-31 2013-05-31 패실로마이세스 속 균주를 이용한 생물 농약 제조방법

Publications (1)

Publication Number Publication Date
WO2014193162A1 true WO2014193162A1 (fr) 2014-12-04

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Family Applications (1)

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PCT/KR2014/004766 WO2014193162A1 (fr) 2013-05-31 2014-05-28 Procédé de préparation d'un biopesticide au moyen d'une souche de paecilomyces sp.

Country Status (3)

Country Link
JP (2) JP2016526043A (fr)
KR (1) KR20140141857A (fr)
WO (1) WO2014193162A1 (fr)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108739804A (zh) * 2018-05-18 2018-11-06 中国农业科学院农业环境与可持续发展研究所 球孢白僵菌可湿性粉剂的制备方法
CN110063330A (zh) * 2019-06-03 2019-07-30 江苏省苏科农化有限责任公司 一种含斯盘80和脂肪胺聚合物绿色环保乳化剂
WO2019238734A1 (fr) * 2018-06-15 2019-12-19 Bayer Aktiengesellschaft Formulations liquides et stables au stockage pour spores fongiques
CN110628651A (zh) * 2019-10-21 2019-12-31 昆明理工大学 一种提高淡紫拟青霉孢子活力的液体深层发酵方法
CN114097828A (zh) * 2021-11-23 2022-03-01 安徽农业大学 一种刀孢蜡蚧菌油剂及其制备方法和应用

Citations (4)

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JP2003063917A (ja) * 2001-08-22 2003-03-05 Dainippon Ink & Chem Inc 糸状菌を有効成分とする製剤の製造方法
JP2004315407A (ja) * 2003-04-15 2004-11-11 Royal Industries Kk 農作物の病害予防及び処置剤並びにそれを用いた病害防止及び処置方法
KR100475131B1 (ko) * 2001-11-02 2005-03-08 한국생명공학연구원 신규한 패실로마이세스 속 미생물 및 그를 포함하는 토양해충 방제용 미생물 살충제
KR20110075132A (ko) * 2009-12-28 2011-07-06 효성오앤비 주식회사 항진균성 바실러스 서브틸리스 균주 및 이를 포함하는 미생물 수화제

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US6506803B1 (en) * 1999-04-28 2003-01-14 Regents Of The University Of Michigan Methods of preventing and treating microbial infections
AU6132700A (en) * 1999-09-30 2001-04-05 Chienna B.V. Polymers loaded with bioactive agents
JP4405718B2 (ja) * 2002-10-02 2010-01-27 クミアイ化学工業株式会社 農業用水和性組成物、その製造方法及びその保存方法
JP2005278526A (ja) * 2004-03-30 2005-10-13 Idemitsu Kosan Co Ltd 植物病害を防除する微生物およびそれを用いた植物病害防除剤。
US7485451B2 (en) * 2004-11-18 2009-02-03 Regents Of The University Of California Storage stable compositions of biological materials
WO2008062413A2 (fr) * 2006-11-21 2008-05-29 Mitam Ltd. Formulations de champignons entomopathogènes pour la lutte contre les insectes
JP2009221132A (ja) * 2008-03-14 2009-10-01 Idemitsu Kosan Co Ltd 農園芸植物の病虫害防除剤

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003063917A (ja) * 2001-08-22 2003-03-05 Dainippon Ink & Chem Inc 糸状菌を有効成分とする製剤の製造方法
KR100475131B1 (ko) * 2001-11-02 2005-03-08 한국생명공학연구원 신규한 패실로마이세스 속 미생물 및 그를 포함하는 토양해충 방제용 미생물 살충제
JP2004315407A (ja) * 2003-04-15 2004-11-11 Royal Industries Kk 農作物の病害予防及び処置剤並びにそれを用いた病害防止及び処置方法
KR20110075132A (ko) * 2009-12-28 2011-07-06 효성오앤비 주식회사 항진균성 바실러스 서브틸리스 균주 및 이를 포함하는 미생물 수화제

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108739804A (zh) * 2018-05-18 2018-11-06 中国农业科学院农业环境与可持续发展研究所 球孢白僵菌可湿性粉剂的制备方法
WO2019238734A1 (fr) * 2018-06-15 2019-12-19 Bayer Aktiengesellschaft Formulations liquides et stables au stockage pour spores fongiques
US20210127686A1 (en) * 2018-06-15 2021-05-06 Bayer Aktiengesellschaft Liquid and storage-stable formulations for fungal spores
CN110063330A (zh) * 2019-06-03 2019-07-30 江苏省苏科农化有限责任公司 一种含斯盘80和脂肪胺聚合物绿色环保乳化剂
CN110628651A (zh) * 2019-10-21 2019-12-31 昆明理工大学 一种提高淡紫拟青霉孢子活力的液体深层发酵方法
CN114097828A (zh) * 2021-11-23 2022-03-01 安徽农业大学 一种刀孢蜡蚧菌油剂及其制备方法和应用

Also Published As

Publication number Publication date
JP6387438B2 (ja) 2018-09-05
JP2016526043A (ja) 2016-09-01
JP2017197550A (ja) 2017-11-02
KR20140141857A (ko) 2014-12-11

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