WO2014147869A1 - Procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, procédé de culture du tissu végétal, agent de stérilisation, agent germicide, et composition de milieu de culture pour la culture d'un tissu végétal - Google Patents

Procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, procédé de culture du tissu végétal, agent de stérilisation, agent germicide, et composition de milieu de culture pour la culture d'un tissu végétal Download PDF

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Publication number
WO2014147869A1
WO2014147869A1 PCT/JP2013/075495 JP2013075495W WO2014147869A1 WO 2014147869 A1 WO2014147869 A1 WO 2014147869A1 JP 2013075495 W JP2013075495 W JP 2013075495W WO 2014147869 A1 WO2014147869 A1 WO 2014147869A1
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Prior art keywords
medium
plant tissue
culture
culture medium
sterilizing agent
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PCT/JP2013/075495
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English (en)
Japanese (ja)
Inventor
洋一 水田
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Mizuta Youichi
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Priority to US14/778,420 priority Critical patent/US20160264932A1/en
Priority to JP2015506539A priority patent/JP6041279B2/ja
Priority to KR1020157030420A priority patent/KR20150132585A/ko
Publication of WO2014147869A1 publication Critical patent/WO2014147869A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/002—Culture media for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0215—Disinfecting agents, e.g. antimicrobials for preserving living parts

Definitions

  • the present invention relates to a method for producing a medium used for culturing plant tissue, a method for culturing plant tissue, a sterilizing agent, a sterilizing agent, and a medium composition for plant tissue culture.
  • a gel or solid medium in which a gelling agent such as agarose or gellan gum is added to the culture solution, or a liquid medium not containing the gelling agent is used.
  • the medium is sterilized in a state of being accommodated in a culture container, and then plant tissue is planted in the medium in a sterile environment (hereinafter referred to as “placement”), and then the plant tissue is cultured in the culture chamber.
  • placement a sterile environment
  • a high-pressure steam sterilizer (autoclave) is used for sterilization of a culture medium and a culture container, and a plant tissue placement work is performed in a sterile room (clean bench). Therefore, when the sterilized medium and the like are taken out from the high-pressure steam sterilizer and moved to the sterilization chamber, careful work must be performed so as not to contaminate bacteria, which is troublesome.
  • the culturable amount is limited to the capacity of the high-pressure steam sterilizer or the sterile room, a large amount of plant tissue cannot be cultured at once. Therefore, research and development of methods that can cultivate plant tissues easily and outdoors without using a high-pressure steam sterilizer or an aseptic room are underway.
  • Non-Patent Document 1 is an academic paper whose author is the inventor of the present application, and discloses a simple plant tissue culture method using a plurality of types of drugs including chlorinated fungicides.
  • the medium is heated and sterilized by adding the drug to the medium dissolved by boiling several times and sterilized by immersing the culture vessel and the plant in a liquid containing the drug, and then in a normal environment.
  • Non-patent document 1 reports that sterilization and sterilization effects comparable to those obtained when using a high-pressure steam sterilizer or a sterile room are obtained.
  • the problem to be solved by the present invention is to cultivate plant tissues that can be easily sterilized or sterilized, and can grow to the same extent as when using an autoclave or a clean bench. It is intended to provide a method for producing a medium, a method for culturing plant tissue, a sterilizing agent for a culture medium for plant tissue, a bactericidal agent for plant bodies, and a medium composition for plant tissue culture.
  • a method for producing a culture medium for plant tissue culture according to the present invention made to solve the above problems, Boiling the medium, A step of adding a first sterilization treatment agent comprising a plurality of kinds of powdered drugs to the medium during boiling; Adding a second sterilization treatment agent comprising one kind of drug to the medium at the end of boiling, and dispensing the medium into a culture container; And a step of cooling the dispensed medium in order.
  • the present invention is characterized in that the first sterilizing agent added during the boiling of the medium is composed of a plurality of kinds of powdered drugs, and the second sterilizing agent added at the end of the boiling is composed of one kind of drug.
  • the first sterilization treatment agent can be prepared in advance because it does not denature even when powdered drugs are mixed together.
  • the method for producing a culture medium for plant tissue Boiling the medium, A step of adding a sterilizing agent comprising a plurality of kinds of powdered drugs to the medium during boiling, and then dispensing the medium into a culture container; This is substantially the same as sequentially performing the step of cooling the dispensed medium.
  • the drug constituting the first sterilization treatment agent preferably contains one or more components selected from sucrose fatty acid esters, nisin, natamycin, ⁇ polylysine, protamines, citrate, and hypochlorite.
  • sucrose fatty acid esters and nisin are preferably included.
  • glycerin fatty acid esters may be included.
  • medical agent which comprises a 2nd sterilization processing agent contains the 1 thru
  • the first and second sterilizing agents it is preferable to use drugs or ingredients that have been designated as food additives or agricultural chemicals.
  • the culture method of the plant tissue based on this invention made in order to solve the said subject, Immersing a culture container containing a solid or semi-solid medium in a first bactericidal composition liquid containing a chlorine-based bactericide for a predetermined time; Immersing the plant tissue in the second bactericidal composition liquid for a predetermined time; Placing the plant tissue extracted from the second sterilizing composition liquid on the medium in the culture container extracted from the first sterilizing composition liquid, and culturing the plant tissue.
  • a medium manufactured by the above-described manufacturing method may be used as the medium in the culture vessel immersed in the first sterilizing composition liquid.
  • the first bactericidal composition liquid preferably contains a drug containing sucrose fatty acid esters in addition to the chlorine-based bactericidal agent.
  • a 2nd disinfection composition liquid contains the chemical
  • drugs containing sucrose fatty acid esters and natamycin are preferably designated as food additives, and drugs containing captan and drugs containing oxolinic acid are both designated as pesticides. It is preferable to have received.
  • CMIT ZeroenC, caisson, etc.
  • the sterilization agent for a culture medium for plant tissue is characterized by comprising a powdery drug containing at least sucrose fatty acid esters and nisin.
  • the plant tissue culture medium composition according to the present invention is characterized by including the sterilizing agent and a powdered medium component.
  • the sterilizing agent used for culturing plant tissue according to the present invention is used for sterilizing a plant body or a culture vessel, and is characterized by mixing a plurality of powdered agents. .
  • the culture container can be simply and without performing troublesome work.
  • the culture medium can be sterilized, and if the obtained culture medium is used, plant tissues can be grown to the same extent as when an autoclave or a clean bench is used.
  • the sterilization treatment can be performed simply by immersing the medium in the first sterilization composition liquid and the plant tissue in the second sterilization composition liquid.
  • the plant tissue cultured by this culture method can be grown to the same extent as when an autoclave or a clean bench is used.
  • the plant tissue culture medium sterilization agent and the plant body sterilization treatment agent and the culture medium composition according to the present invention are premixed with components necessary for the medium sterilization treatment and plant body sterilization treatment. Therefore, the medium and the plant can be easily sterilized and sterilized.
  • Explanatory drawing of the sterilization procedure of a solid medium (2 times addition type).
  • Explanatory drawing of the sterilization procedure of a solid culture medium (single addition type).
  • Explanatory drawing of the sterilization procedure of a solid culture medium (medium component mixing type).
  • cultivation procedure using a solid medium Explanatory drawing of the culture
  • (1-2) A method of sterilizing by adding a sterilizing agent once when the medium is solidified (hereinafter referred to as “single addition type”, see FIG. 2) 1.
  • the medium is heated to boiling (boiling). 2.
  • a 1st sterilization processing agent a component is mentioned later
  • dissolved culture medium a culture medium is dispensed to a culture container and it cools at room temperature.
  • (1-3) A method in which a sterilizing agent is mixed in advance with medium components (hereinafter referred to as “medium component mixed type”, see FIG. 3).
  • medium component mixed type medium components
  • the sterilized solid medium contained in the culture container is the second sterilization treatment solution (P-1670 is selected from 2000 mg / L (SE-P2000), Chemomicron G is selected from 1400 mg / L (Cl1400) or both. Soak in). 3. After taking out the culture vessel from the second sterilization treatment composition solution, it is turned over and the excess solution is discharged. 4). The explant obtained in the procedure 1 is placed on the medium in the culture vessel obtained in the procedure 3, and cultured in a normal laboratory.
  • Nisaprine (Nisin) had no adverse effects on growth at concentrations below 500 mg / L.
  • Chemomicron G (Cl) had no adverse effect on growth at a concentration of 14 mg / L or less.
  • the effective chlorine concentration of Chemmicron G14mg / L is 10mg / L. Based on the above results, the upper limit value of the drug concentration used for each sterilization agent and sterilization solution was set.
  • Solid medium Otsuka
  • a prescription Sterilization method Autoclave Bacterial inoculation method Culture: Ground surface [B5, S3: 50 ⁇ L / test tube drop of bacterial suspension on medium surface, An: Conidia powder or conidia powder pregelatinized starch 10 mg / tube into the surface of the medium diluted at the specified magnification in the above], explant [B5, S3: immersed in bacterial suspension (10 ⁇ L attached per explant), An: conidial powder or conidia Powder inoculated powder diluted with pregelatinized starch at a specified magnification (5 mg adhered per explant)]
  • Explant Potato Placement method: Method shown in Fig. 4 Culture period: 14 days
  • FIG. 12 shows the results when Otsuka A-prescribed liquid medium was used as the liquid medium and cultured for 30 days. From Fig. 12, impact N is not adversely affected if the concentration is 500 mg / L or less, orthoside 80 does not adversely affect the growth if the concentration is 50 mg / L or less, and Chemomicon G is autoclaved if the concentration is 7 mg / L or less. It was found that a sterilization effect equivalent to or higher than that can be obtained.
  • the microbial contamination rate was compared between the case where the bacteria were inoculated into the medium and the case where the bacteria were inoculated into the explants.
  • the inoculation concentration of the fungus, the type of plant, etc. are as follows.
  • the microbial contamination rate was higher in the inoculated explant than in the inoculated medium.
  • An's microbial contamination rate is high and it is difficult to prevent contamination.
  • the culture medium, culture vessel, culture conditions, etc. used for the culture are as follows.
  • Medium Otsuka A prescription equal time + ammonium sulfate 0.5 g / L, sucrose 15 g / L, gellan gum 2 g / L Add 2.5 g / L for yeast extract, 5 g / L for peptone, and add 75 g / L of pulverized mixer to the culture medium.
  • Culture vessel 23 mm inner diameter test tube and plastic molton stopper (3) Medium volume 30 mL, 1 explant per culture container
  • the culture was performed in either a single addition type or a double addition type.
  • Example 1 It consists of the following procedures. 1. After the medium is heated to boiling (boiling), a first sterilizing agent consisting of a mixture of SE-P: 0.2 g / L and nisin: 0.2 g / L is added to the dissolved medium. SE-P and nisin are both powdered drugs. Continue boiling for 2.2 minutes. 3. After adding Cl: 3 mg / L as the second sterilizing agent, the medium is dispensed into a culture container and cooled at room temperature to solidify the medium.
  • Example 2 The procedure is the same as that of Example 1 except that the duration of boiling is 5 minutes. 1. After the medium is boiled, a first sterilizing agent consisting of a mixture of SE-P: 0.2 g / L and nisin: 0.2 g / L is added. Continue boiling for 2.5 minutes. 3. After adding Cl: 3 mg / L, dispense the medium into the culture vessel and cool at room temperature.
  • Example 3 The procedure is the same as that of Example 1 except that the duration of boiling is 10 minutes, and the procedure is as follows. 1. After the medium is boiled, a first sterilizing agent consisting of a mixture of SE-P: 0.2 g / L and nisin: 0.2 g / L A: 5 mg / L is added. 2. Continue boiling for 10 minutes. 3. After adding Cl: 2 mg / L, the medium is dispensed into culture vessels and cooled at room temperature.
  • Example 4 The procedure is the same as that of Example 1 except that the boiling time is 30 minutes, and the procedure is as follows. 1. After the medium is boiled, a first sterilizing agent consisting of a mixture of SE-P: 0.2 g / L and nisin: 0.2 g / L is added. 2. Continue boiling for 30 minutes. 3. After adding Cl: 2 mg / L, the medium is dispensed into culture vessels and cooled at room temperature.
  • the comparative example is almost the same as the procedure of Examples 1 to 4 except that the two kinds of drugs were weighed and added after boiling of the medium and after the boiling continued.
  • ⁇ Comparative Example 1> 1. The medium is heated and boiled, and then Cl: 1.4 mg / L and SE-L: 0.5 g / L are weighed and added. 2. Continue boiling for 3 minutes. 3. Cl: 1.4 mg / L and nisin: 0.2 g / L L are weighed and added, and then the medium is dispensed into a culture vessel and cooled at room temperature.
  • FIG. 15 shows the results of culturing for 30 days by the culture method described above using the media produced by the methods of Examples 1 to 4 and Comparative Examples 1 to 4 described above.
  • Comparative Examples 1 and 2 are conventional medium sterilization methods developed by the present inventor, and none of the test tubes in which spore colonies were formed produced a sufficient sterilization effect.
  • none of the drugs used in Comparative Examples 1 and 2 can be mixed in advance.
  • SE-L used as the SE source in Comparative Example 1 is a viscous liquid.
  • Nisin is a powder, but simultaneously added Cl is a strong oxidizable solid and may be denatured when mixed.
  • SE-P used as the SE source in Comparative Example 2 is a powder, but it cannot be mixed in advance because Cl added simultaneously is a strong oxidizing solid. Therefore, at the time of boiling, it is necessary to weigh and add two kinds of chemicals after boiling continues, which takes time.
  • Comparative Example 3 is an example in which all the agents are added at once, thereby reducing labor, but in this example, spore colonies are formed in several media, Comparative Example 1, Compared to 2, the sterilization effect decreased.
  • the food additive is used as a sterilizing agent for the medium, but an agrochemical may be used. Furthermore, the following modifications are also possible.
  • ⁇ Modification 1> 1 After the medium is boiled, a mixture of P-1670: nisapurine: natamycin: stana wettable powder (pesticide): dextrin in a weight ratio of 100: 100: 10: 1: 39 is added at 0.25 g / L. According to this modified example 1, it is possible to reduce the invasion of bacteria into the culture medium after cooling and solidification, and there is no need to keep the culture container sealed.
  • ⁇ Modification 2> 1 P-1670: Nisapurin: Natamycin: Stana wettable powder (pesticide): Dextrin by weight ratio of 100: 100: 10: 1: 39 0.25g / L, granulated sugar 20g / L, gellan gum 2g / L, Otsuka Add 1 L of boiling water to 25 g of a mixture of House 1 No. 1.5 g / L and Otsuka House No. 2 1 g / L, stir well and dissolve. According to the second modification, a sterilized medium can be created simply by pouring hot water into the powder.
  • ⁇ Modification 3> This is a medium sterilization method suitable for liquid culture at room temperature.
  • the weight ratio of P-1670: Nisapurin: Natamycin: Impact N: Orthoside 80: Stana wettable powder (pesticide) to medium at room temperature Add a mixture of 4: 4: 20: 51: 20: 1 at 0.25 g / L and 7 mg / L of CHEMIKRON G and seal.
  • a simple plant culture medium containing major salts, vitamins, and plant regulators can be sterilized in a situation where heat-resistant spores are contaminated to about 10,000 CFU / L.
  • ⁇ Modification 5> This is a sterilization method for plant tissues, which uses food additives. Specifically, the plant is immersed in an aqueous solution containing 1 g / L of P-1670, 10 g / L of natamycin, and 10 g / L of nisapurine, and then introduced into the medium sterilized in Example 5 or Modification 2. Seal the container. However, in the case of Example 5, the medium is immersed in a solution containing 1 g / L of P-1670 and 1.43 g / L of Chemmicron G together with the container. This method can be sterilized to an extent sufficient for aseptic culture of explants without using a clean bench.

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Abstract

Cette invention concerne un procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, caractérisé en ce qu'il comprend : une étape consistant à porter un milieu de culture à ébullition ; une étape consistant à ajouter un premier agent de stérilisation, qui comprend de multiples types de produits chimiques à l'état pulvérulent, au milieu de culture pendant l'ébullition ; une étape consistant à ajouter un second agent de stérilisation, qui comprend un seul type de produit chimique, au milieu de culture au point d'achèvement de l'ébullition, puis à distribuer le milieu de culture dans des récipients de culture ; une étape consistant à refroidir le milieu de culture distribué ; une étape consistant à humidifier le milieu de culture refroidi et le tissu végétal à l'aide de multiples types de produits chimiques liquides ; et une étape consistant à placer le tissu végétal dans le milieu de culture, lesdites étapes étant exécutées dans cet ordre. Par conséquent, la présente invention concerne un procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, qui permet la mise en œuvre facile d'un traitement de stérilisation et d'un traitement germicide et la croissance du tissu végétal au même niveau en cas d'utilisation d'un autoclave ou d'un banc stérile, et un procédé d'ancrage d'un tissu végétal.
PCT/JP2013/075495 2013-03-22 2013-09-20 Procédé de préparation d'un milieu de culture pour la culture d'un tissu végétal, procédé de culture du tissu végétal, agent de stérilisation, agent germicide, et composition de milieu de culture pour la culture d'un tissu végétal WO2014147869A1 (fr)

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US14/778,420 US20160264932A1 (en) 2013-09-20 2013-09-20 Method for preparing culture medium for culturing plant tissue, method for culturing plant tissue, sterilizing agent, microbicidal agent, and culture medium composition for culturing plant tissue
JP2015506539A JP6041279B2 (ja) 2013-03-22 2013-09-20 植物組織の培養用培地の製造方法及び植物組織の培養方法並びに滅菌処理剤、殺菌処理剤、及び植物組織培養用培地組成物
KR1020157030420A KR20150132585A (ko) 2013-03-22 2013-09-20 식물 조직의 배양용 배지의 제조 방법 및 식물 조직의 배양 방법 및 멸균 처리제, 살균 처리제, 및 식물 조직 배양용 배지 조성물

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Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012505648A (ja) * 2008-10-17 2012-03-08 ビオメリュー レジオネラ属バクテリアを検出および／または同定するための反応培地

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3648608B2 (ja) * 2002-06-12 2005-05-18 独立行政法人理化学研究所 植物用防カビ剤及びカビ発生防止方法
DE602004030790D1 (de) * 2003-06-16 2011-02-10 Monsanto Technology Llc Verfahren und gerät zur herstellung von genetisch transformierbarem pflanzengewebe
GB201116010D0 (en) * 2011-09-15 2011-10-26 Algipharma As Use of alginate oligomers to enhance the effects of antifungal agents

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012505648A (ja) * 2008-10-17 2012-03-08 ビオメリュー レジオネラ属バクテリアを検出および／または同定するための反応培地

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
YOICHI MIZUTA ET AL.: "Autoclave to Clean Bench o Mochiinai Kan'i Soshiki Baiyo ni Okeru Biseibutsu Osenritsu no Teigen", HORTICULTURAL RESEARCH ( JAPAN, vol. 9, 2, 2010, pages 298 *
YOICHI MIZUTA ET AL.: "Hikanetsu Mekkinho o Mochiita Kan'i Ekitai Baiyo ni Okeru Biseibutsu Osen no Teigen to Shokubutsu no Seiiku Sokushin", HORTICULTURAL RESEARCH ( JAPAN, vol. 8, 2, 2009, pages 308 *
YOICHI MIZUTA ET AL.: "Kokin Busshitsu Shorigo ni Gaishokutai o Enso Yoeki de Nuraseba Clean Bench Fuyo de Ichido ni Tasu Chisho Dekiru", JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, vol. 75, 1, 2006, pages 188 *
YOICHI MIZUTA ET AL.: "Mekkin Yoki to Teinodo no Sakkinzai o Mochiita Autoclave Mekkin Fuyo Kan'i Baiyoho", JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, vol. 70, 2, 2001, pages 140 *
YOICHI MIZUTA ET AL.: "Shitsunai deno Baichi Mekkin to Gaishokutai Chisho ni Oite Sushu no Yakuzai no Heiyo ni yori Biseibutsu Josenritsu to Kanbensei ga Kojo suru", HORTICULTURAL RESEARCH ( JAPAN, vol. 11, 2, 22 September 2012 (2012-09-22), pages 501 *
YOICHI MIZUTA ET AL.: "Shokuhin Tenkabutsu to Shafutsu o Heiyo shite Baichi o Mekkin suru to Chokikan no Shokubutsu Baiyo demo Biseibutsu Osen sarenai", JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, vol. 75, 2, 2006, pages 311 *

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