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• • A—HUMAN NECESSITIES
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• • A—HUMAN NECESSITIES
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• • A—HUMAN NECESSITIES
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  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
  • A61P3/06—Antihyperlipidemics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P3/00—Drugs for disorders of the metabolism
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  • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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  • C12Q2600/00—Oligonucleotides characterized by their use
  • C12Q2600/158—Expression markers
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• • G—PHYSICS
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  • G01N2800/08—Hepato-biliairy disorders other than hepatitis

Definitions

• the invention relates to variants of fibroblast growth factor 19 (FGF19) proteins and peptide sequences (and peptidomimetics) and fusions of FGF19 and/or fibroblast growth factor 21 (FGF21) proteins and peptide sequences (and peptidomimetics), and variants of fusions of FGF19 and/or FGF21 proteins and peptide sequences (and peptidomimetics) that modulate bile acid homeostasis, and methods for and uses of the variants and fusions in treatment of bile acid related and associated disorders.
• FGF19 fibroblast growth factor 19
• FGF21 fibroblast growth factor 21
• Bile acids steroid acids that are found predominantly in the bile of mammals, regulate cholesterol, triglyceride, glucose and energy homeostasis, and facilitate digestion and absorption of lipids in the small intestine. Emulsification of lipids and fat-soluble vitamins in the intestine allows the formation of micelles that can then be transported via the lacteal system. Other functions of bile acids include driving the flow of bile to eliminate catabolites from the liver and aiding in the reduction of the bacteria flora found in the small intestine and biliary tract. Bile acids are also involved in the regulation of their own synthesis and enterohepatic circulation. See, e.g., Staels et al, Diabetes Care (2009) vol. 32 no. suppl 2 S237-S245.
• bile acid production occurs primarily in the perivenous hepatocytes through a series of enzymatic reactions that convert cholesterol into the two primary bile acids, cholic acid and chenodeoxycholic acid.
• the primary bile acids are synthesized by two distinct pathways. In the "classic” or “neutral” pathway, the primary bile acids are produced by hydroxylation of cholesterol through catalysis by the cytochrome P450 enzyme cholesterol 7a-hydroxylase (cyp7al), which catalyzes the first and rate-limiting step in the classical bile acid synthesis pathway. (See, e.g., Inagaki et al, Cell Metabolism 2:217-25 (Oct 2005)).
• cyp7al activity of cyp7al is down-regulated by cholic acid and up- regulated by cholesterol; thus, cyp7al is regulated by bile acids themselves.
• the conversion of cholesterol to bile acids is primarily effected by this pathway.
• This pathway is regulated by the enzyme cyp27al, which converts oxysterols to bile acids.
• cyp27al is not regulated by bile acids themselves.
• cholic acid and chenodeoxycholic acid are secreted into the lumen of the intestine, intestinal bacteria dehydroxylate a portion of each to form the secondary bile acids, deoxycholic acid (derived from cholic acid) and lithocholic acid (derived from chenodeoxycholic acid).
• Hepatic cells may conjugate these four bile with one of two amino acids, glycine or taurine, to form a total of eight possible conjugated bile acids, referred to as bile salts.
• the principal bile acids are cholic acid, chenodeoxycholic acid, glycocholic acid, taurocholic acid, deoxycholic acid and lithocholic acid.
• All four of these bile acids can be transported back into the blood stream, be returned to the liver, and be re-secreted through enterohepatic circulation. See, e.g., Staels et ah, Diabetes Care (2009) vol. 32 suppl 2 S237-S245.
• the primary bile acids (cholic acid and chenodeoxycholic acid) are synthesized in the liver), while the secondary bile acids (deoxycholic acid and lithocholic acid) are made by bacteria.
• the four bile acids are secreted into the bile canalicular lumen for storage in the gallbladder as mixed micelles with phospholipids and cholesterol.
• cholecystokinin stimulates gallbladder contraction resulting in its release of micellar bile acids into the intestinal lumen to aid digestion.
• Enterohepatic circulation enables -90-95% of bile acids to be reabsorbed from the distal ileum and transported back to the liver; this bile acid uptake and transportation occurs primarily by pericentral hepatocytes.
• the approximately 5% of bile acids that are not reabsorbed are eliminated in the feces, and that amount of loss is subsequently replaced by de novo bile acid synthesis in the liver.
• the primary bile acids are physiological ligands/activators of farnesoid-X-receptor (FXR), pregnane -X-receptor (PXR) and constitutive androstane receptor (CAR), and litocholic acid is a ligand for the Vitamin D receptor (VDR) and the
• FXR demonstrates a high selectivity for bile acids
• PXR and CAR act upon a number of receptors integrating lipid homeostasis with xenobiotic metabolism.
• FXR, PXR, CAR and TGR5 exert synergistic activities in regulating lipid and glucose homeostasis and energy expenditure, as well as in regulating liver and peripheral insulin sensitivity.
• bile acids are potentially toxic to cells, and the size of the bile acid pool is tightly regulated within the liver and intestine to prevent cytotoxic accumulation.
• FXR nuclear receptors
• FGF 19 is secreted from the intestine and signals to the liver to repress Cyp7al .
• intestinal FXR activation due to transintestinal bile acid flux after a meal also induces the expression of FGF 19, which is released by small intestine epithelial cells and circulates to bind to hepatocyte FGF receptor 4 (FGFR4) receptors; the FGFR4 receptors signal a reduction in bile acid synthesis via c-Jun NH 2 -terminal kinase (JNK) pathway activation.
• JNK c-Jun NH 2 -terminal kinase
• abnormal bile acid homeostasis can result in, or exacerbate, a number of disorders, including cholestasis, portosystemic shunt, Crohn's disease, and hepatic microvascular dysplasia.
• bile acids play a role in modulating the metabolic syndrome, a cluster of cardiovascular disease risk factors that include visceral obesity, insulin resistance, dyslipidemia, increased blood pressure, and hypercoagulability.
• modulation of bile acid activity can provide a number of beneficial therapeutic effects.
• Activation of FXR by bile acids lowers plasma triglycerides and has been shown to improve hyperglycemia in diabetic mice.
• Bile acids may also regulate energy expenditure in an FXR-independent manner in mice through activation of the G protein-coupled receptor TGR5.
• modulation of FXR activity and bile acid metabolism may provide a therapeutic approach for the treatment of, for example, the metabolic syndrome and diabetes type 2. See, e.g. , Lefebvre et al, Physiol Rev. 2009 Jan;89(l): 147-91.
• Bile acid synthesis disrupts the enterohepatic circulation of bile acids, decreases plasma total and LDL cholesterol, and increases levels of HDL cholesterol, apohpoprotein (apo)-AI, and triglycerides.
• apo apohpoprotein
• triglycerides As a direct consequence of interrupting the return of bile acids to the liver, cyp7al expression becomes de-repressed, and conversion of cholesterol into bile acids is stimulated.
• agents that sequester bile acids in the gut ⁇ e.g. , cholestyramine
• prevent their reabsorption prevent their reabsorption, resulting in, as a compensatory mechanism, more endogenous cholesterol being shunted into the production of bile acids, leading to reduced cholesterol levels.
• bile acid synthesis has also been found to be associated with type 2 diabetes.
• a number of factors may contribute to glucose regulation, including effects on bile acid pool size and composition, FXR-mediated alterations in hepatic glucose production and intestinal glucose absorption, influences on peripheral insulin sensitivity, incretin effects, and energy use.
• modulation of bile acid synthesis useful in the treatment of diabetes, it may also find clinical utility in the treatment of pre-diabetes.
• bile acid diarrhea a condition referred to as a bile acid diarrhea (BAD). More particularly, when intestinal expression of the bile acid transporters is reduced, the intestine is less efficient at bile acid reabsorption (Type 1 bile acid malabsorption). Similarly, if intestinal motility is affected by gastro-intestinal surgery, or bile acids are deconjugated by small intestinal bacterial overgrowth, absorption is less efficient (Type 3 bile acid malabsorption).
• cholestasis The condition of cholestasis is caused by acute or chronic interruption in the excretion of bile (through, for example, obstruction) within or outside the liver. Failure to form bile results in progressive cholestatic liver injury and death. Obstruction causes bile salts, the bile pigment bilirubin, and lipids to accumulate in the blood stream instead of being eliminated normally.
• Symptoms of chronic cholestasis include skin discoloration, scars or skin injuries caused by scratching, bone pain, xanthoma, or xanthelasma. Patients with advanced cholestasis feel ill, tire easily, and are often nauseated. Abdominal pain and such systemic symptoms as anorexia, vomiting, and fever are usually due to the underlying condition that causes cholestasis.
• Intrahepatic cholestasis is usually caused by hepatitis or by medications that produce symptoms resembling hepatitis. Phenothiazine-derivative agents, including chlorpromazine, can cause sudden fever and inflammation, although symptoms usually disappear after cessation of the agents. In rare cases, a condition resembling chronic biliary cirrhosis, discussed further below, persists even after the medication is stopped. Some patients experience a similar reaction in response to, for example, tricyclic antidepressants (e.g., amitriptyline and imipramine) and phenylbutazone. Intrahepatic cholestasis may also have other causes, including alcoholic liver disease, primary biliary cirrhosis, and cancer that has metastasized.
• Phenothiazine-derivative agents including chlorpromazine
• Extrahepatic cholestasis can be caused by conditions such as tumors and gallstones that block the flow of bile from the gallbladder to the duodenum (e.g., by a stone obstructing the common bile duct). Extrahepatic cholestasis may also be caused by pancreatic cancer and, less frequently, as a result of non-cancerous narrowing of the common duct, ductal carcinoma, or disorders of the pancreas.
• Symptoms of both intrahepatic and extrahepatic cholestasis include jaundice, dark urine, and pale stools. Itching over the skin may be severe if the condition is advanced.
• Intrahepatic cholestasis of pregnancy frequently develops during the second and third trimesters of pregnancy, and it is the second most common cause of jaundice during pregnancy. Although symptoms usually disappear within two-to-four weeks after the baby's birth, they may reappear if the mother subsequently becomes again. A similar condition affects some women who take oral contraceptives, but symptoms disappear upon cessation of the use of oral contraceptives.
• Drug-induced cholestasis may be a complication of chemotherapy or other medications.
• the two major types of drug-induced cholestasis are idiosyncratic reactions and direct toxic injury. Idiosyncratic reactions may occur at the onset of treatment or thereafter. Allergic responses are varied and are not related to the amount of medication being taken.
• PBC Primary Biliary Cirrhosis
• PBC cholestatic liver disease
• a majority of PBC patients are asymptomatic at the time of initial diagnosis, but most develop symptoms, such as fatigue and pruritus, over time. Jaundice may result from advanced disease.
• a liver biopsy can be used to confirm the diagnosis of PBC, and bilirubin is frequently monitored to provide an indication of liver function. Elevated serum levels of ALP, an enzyme released by hepatic cells in response to bile acid- mediated toxicity, is generally closely monitored in patients as an indicator of treatment response and prognosis.
• OCA is a bile acid analog and FXR agonist derived from the primary human bile acid chenodeoxycholic acid, or CDCA.
• OCA is being evaluated for patients having an inadequate therapeutic response to ursodiol or who are unable to tolerate ursodiol (Intercept Pharmaceuticals, New York).
• Primary sclerosing cholangitis is a chronic fibrosing inflammatory process that results in the destruction of the biliary tree and biliary cirrhosis.
• the strictures are located in both the intrahepatic and extrahepatic ducts in more than 80% of the patients, but about 10% of these patients have only intrahepatic strictures, while less than 5% will have only extrahepatic strictures.
• Bile acid malabsorption is readily diagnosed by the SeHCAT (23-seleno-25-homo-tauro- cholic acid (selenium homocholic acid taurine or tauroselcholic acid)) nuclear medicine test.
• SeHCAT 23-seleno-25-homo-tauro- cholic acid (selenium homocholic acid taurine or tauroselcholic acid) nuclear medicine test.
• An alternative diagnostic test involves measurement in the serum of 7 alpha-hydroxy-4-cholesten-3-one, a bile acid precursor.
• Bile acid sequestrants ⁇ e.g., cholestyramine and colestipol which are in powder form) are the main agents used to treat bile acid malabsorption. Unfortunately, many patients do not tolerate cholestyramine and colestipol, often because of the poor texture and taste of the resin powder.
• bile acid sequestrant colesevelam is available in tablet form and is often better tolerated.
• All bile acid sequestrants are capable of binding other compounds, and it is also possible that deficiencies of fat-soluble vitamins (A, D, E and K) may occur, requiring administration of vitamin supplements.
• Displacement and replacement therapy have also proven useful in certain disorders associated with bile acid homeostasis.
• displacement therapy the composition of the circulating bile acids is changed, either to decrease the cytotoxicity of endogenous bile acids or to modulate cholesterol metabolism to decrease biliary cholesterol secretion.
• bile acid replacement aims to correct a bile acid deficiency.
• UDCA therapy has been shown to improve liver test results in patients with primary biliary cirrhosis, an effect that likely involves multiple mechanisms. UDCA therapy has also shown favorable effects in other cholestatic conditions, such as cholestasis associated with pregnancy and cholestasis associated with total parenteral nutrition.
• Bile acid replacement is used in inborn errors of bile acid biosynthesis, usually with a mixture of chenodeoxycholic Acid (CDCA) or Ursodeoxycholic Acid (UDCA) and cholic acid, to suppress the synthesis of cytotoxic bile acid precursors and restore the input of primary bile acids into the enterohepatic circulation.
• CDCA chenodeoxycholic Acid
• UDCA Ursodeoxycholic Acid
• treatment of PBC generally entails administration of ursodiol, though alternative therapies are being evaluated for patients having an inadequate therapeutic response to ursodiol or who are unable to tolerate ursodiol.
• bile acid disorders such as the foregoing disorders and including, but not limited to: metabolic syndrome; a lipid or glucose disorder;
• cholestasis including, for example diseases of intrahepatic cholestasis (e.g., primary biliary cirrhosis (PBC), primary familial intrahepatic cholestasis (PFIC) (e.g., progressive PFIC), primary sclerosing choangitis (PSC), pregnancy intrahepatic cholestasis (PIC), neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic))
• PBC primary biliary cirrhosis
• the invention is based, in part, on variants of FGF19 peptide sequences, fusions of FGF19 and/or FGF21 peptide sequences and variants of fusions (chimeras) of FGF19 and/or FGF21 peptide sequences having one or more activities, such as bile acid homeostasis modulating activity.
• variants and fusions (chimeras) of FGF19 and/or FGF21 peptide sequences include sequences that are used for treating a bile-acid related or associated disorder.
• variants and fusions (chimeras) of FGF19 and/or FGF21 peptide sequences also include sequences that do not substantially or significantly increase or induce hepatocellular carcinoma (HCC) formation or HCC tumorigenesis.
• variants and fusions (chimeras) of FGF19 and/or FGF21 peptide sequences further include sequences that do not induce a substantial elevation or increase in lipid profile.
• a method or use of modulating bile acid homeostasis or treating a bile-acid related or associated disorder includes: administering a chimeric peptide sequence, comprising: a) an N-terminal region comprising at least seven amino acid residues, the N-terminal region having a first amino acid position and a last amino acid position, wherein the N-terminal region comprises DSSPL or DASPH; and b) a C-terminal region comprising a portion of SEQ ID NO: 99 [FGF19], the C-terminal region having a first amino acid position and a last amino acid position, wherein the C-terminal region comprises amino acid residues 16-29 of SEQ ID NO: 99 [FGF19] (WGDPIRLRHLYTSG; SEQ ID NO: 169), wherein the W residue corresponds to the first amino acid position of the C-terminal region, to modulate bile acid homeostasis or treat the bile-acid related or associated disorder.
• a method or use of modulating bile acid homeostasis or treating a bile-acid related or associated disorder includes: administering a chimeric peptide sequence, comprising: a) an N-terminal region comprising a portion of SEQ ID NO: 100 [FGF21], the N- terminal region having a first amino acid position and a last amino acid position, wherein the N- terminal region comprises amino acid residues GQV, and wherein the V residue corresponds to the last amino acid position of the N-terminal region; and b) a C-terminal region comprising a portion of SEQ ID NO: 99 [FGF19], the C-terminal region having a first amino acid position and a last amino acid position, wherein the C-terminal region comprises amino acid residues 21-29 of SEQ ID NO:99 [FGF19], PvLPvHLYTSG (SEQ ID NO: 185), and wherein the R residue corresponds to the first position of the C-terminal region, to
• a method or use of modulating bile acid homeostasis or treating a bile-acid related or associated disorder includes: administering a chimeric peptide sequence, comprising: a) an N-terminal region comprising a portion of SEQ ID NO: 100 [FGF21], the N- terminal region having a first amino acid position and a last amino acid position,
• N-terminal region comprises at least 5 contiguous amino acids of SEQ ID NO: 100
• [FGF21] including the amino acid residues GQV, and wherein the V residue corresponds to the last amino acid position of the N-terminal region; and b) a C-terminal region comprising a portion of SEQ ID NO: 99 [FGF19], the C-terminal region having a first amino acid position and a last amino acid position, wherein the C-terminal region comprises amino acid residues 21-29 of SEQ ID NO:99 [FGF19], RLRHLYTSG (SEQ ID NO: 185), and wherein the R residue corresponds to the first position of the C-terminal region, to modulate bile acid homeostasis or treat the bile-acid related or associated disorder.
• a method or use of modulating bile acid homeostasis or treating a bile-acid related or associated disorder includes: administering a peptide sequence, comprising or consisting of any of: a) a FGF19 sequence variant having one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF19; b) a FGF21 sequence variant having one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF21; c) a portion of an FGF19 sequence fused to a portion of an FGF21 sequence; or d) a portion of an FGF19 sequence fused to a portion of an FGF21 sequence, wherein the FGF19 and/or FGF21 sequence portion(s) have one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF19 and/or FGF21, to modulate bile acid homeostasis or treat the bil
• a chimeric peptide sequence has an N-terminal region with at least 6 contiguous amino acids of SEQ ID NO: 100 [FGF21] including the amino acid residues GQ; or has an N-terminal region with at least 7 contiguous amino acids of SEQ ID NO: 100 [FGF21] including the amino acid residues GQV.
• a peptide sequence has amino-terminal amino acids 1-16 of SEQ ID NO: 100 [FGF21] fused to carboxy-terminal amino acids 21-194 of SEQ ID NO:99 [FGF19], or the peptide sequence has amino-terminal amino acids 1-147 of SEQ ID NO: 99 [FGF19] fused to carboxy-terminal amino acids 147-181 of SEQ ID NO: 100 [FGF21] (M41), or the peptide sequence has amino-terminal amino acids 1-20 of SEQ ID NO: 99 [FGF19] fused to carboxy-terminal amino acids 17-181 of SEQ ID NO: 100 [FGF21] (M44), or the peptide sequence has amino-terminal amino acids 1-146 of SEQ ID NO: 100 [FGF21] fused to carboxy-terminal amino acids 148-194 of SEQ ID NO:99 [FGF19] (M45), or the peptide sequence has amino-terminal amino acids 1-20 of SEQ ID NO: 99 [
• a peptide sequence has at least one amino acid substitution to amino acid residues 125-129 of SEQ ID NO:99 [FGF19], EIRPD; at least one amino acid substitution to amino acid residues 126-128 of SEQ ID NO:99 [FGF19], IRP; or at least one amino acid substitution to amino acid residues 127-128 of SEQ ID NO: 99 [FGF19], RP, or at least one amino acid substitution to amino acid residues 1-124 of SEQ ID NO: 99 [FGF19] and/or to amino acid residues 130-194 of SEQ ID NO:99 [FGF19]. More specifically, for example, a peptide sequence with a substitution to one of amino acid residues 127-128 of SEQ ID NO: 99 [FGF19], IRP, wherein at least one amino acid substitution is R127L or P128E.
• Methods and uses of the invention can be practiced using a peptide or chimeric sequence, as set forth herein.
• a sequence that includes or consists of any peptide sequence set forth herein as Ml to M98, or MlOl to M160, or SEQ ID NOs: l to 98, 101 to 135, or 138 to 196 a peptide sequence that includes or consists of any sequence set forth in Tables 1-10, or a peptide sequence that includes or consists of any sequence set forth in the Sequence Listing herein.
• Methods and uses of the invention can be practiced using a peptide or chimeric sequence of any suitable length.
• the N-terminal or C-terminal region of the peptide or chimeric sequence is from about 20 to about 200 amino acid residues in length.
• a peptide or chimeric sequence has 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18,
• a peptide or chimeric sequence has an N-terminal region, or a C- terminal region that includes or consists of an amino acid sequence of about 5 to 10, 10 to 20, 20 to 30, 30 to 40, 40 to 50, 60 to 70, 70 to 80, 80 to 90, 90 to 100 or more amino acids.
• a peptide or chimeric sequence has an FGF19 sequence portion, or an FGF21 sequence portion that includes or consists of an amino acid sequence of about 5 to 10, 10 to
• a peptide sequence has: a WGDPI (SEQ ID NO: 170) sequence motif corresponding to the WGDPI sequence of amino acids 16-20 of SEQ ID NO: 99 [FGF19]; has a substituted, mutated or absent WGDPI (SEQ ID NO: 170) sequence motif corresponding to FGF19 WGDPI (SEQ ID NO: 170) sequence of amino acids 16-20 of FGF19; has a WGDPI (SEQ ID NO: 170) sequence with one or more amino acids substituted, mutated or absent.
• the peptide sequence is distinct from an FGF 19 variant sequence having any of GQV, GDI, WGPI (SEQ ID NO: 171), WGDPV (SEQ ID NO: 172), WGDI (SEQ ID NO: 173), GDPI (SEQ ID NO: 174), GPI, WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 178), WGDAI (SEQ ID NO: 179), WGDPA (SEQ ID NO: 180), WDPI (SEQ ID NO: 181), WGDI (SEQ ID NO: 182), WGDP (SEQ ID NO: 183) or FGDPI (SEQ ID NO: 184) substituted for the FGF 19 WGDPI (SEQ ID NO: 170) sequence at amino acids 16-20.
• the N-terminal region comprises amino acid residues VHYG (SEQ ID NO: 101), wherein the N-terminal region comprises amino acid residues DASPHVHYG (SEQ ID NO: 102), or the N-terminal region comprises amino acid residues DSSPLVHYG (SEQ ID NO: 103). More particularly, in one aspect the G corresponds to the last position of the N-terminal region.
• the N-terminal region comprises amino acid residues DSSPLLQ (SEQ ID NO: 104), where the Q residue is the last amino acid position of the N-terminal region, or comprises amino acid residues DSSPLLQFGGQV (SEQ ID NO: 105), where the V residue corresponds to the last position of the N-terminal region.
• an N-terminal region further includes: RHPIP (SEQ ID NO: 106), where R is the first amino acid position of the N-terminal region; or HPIP (SEQ ID NO: 107), where H is the first amino acid position of the N-terminal region; or RPLAF (SEQ ID NO: 108), where R is the first amino acid position of the N-terminal region; or PLAF (SEQ ID NO: 109), where P is the first amino acid position of the N-terminal region; or R, where R is the first amino acid position of the N-terminal region.
• RHPIP SEQ ID NO: 106
• HPIP SEQ ID NO: 107
• H the first amino acid position of the N-terminal region
• RPLAF SEQ ID NO: 108
• PLAF SEQ ID NO: 109
• a peptide or chimeric sequence has: amino acid residues HPIP (SEQ ID NO: 107), which are the first 4 amino acid residues of the N-terminal region.
• a peptide or chimeric sequence has: an R residue at the first position of the N- terminal region, or the first position of the N-terminal region is an M residue, or the first and second positions of the N-terminal region is an MR sequence, or the first and second positions of the N- terminal region is an RM sequence, or the first and second positions of the N-terminal region is an RD sequence, or the first and second positions of the N-terminal region is an DS sequence, or the first and second positions of the N-terminal region is an MD sequence, or the first and second positions of the N-terminal region is an MS sequence, or the first through third positions of the N- terminal region is an MDS sequence, or the first through third positions of the N-terminal region is an RDS sequence, or the first through third positions
• a peptide or chimeric sequence has at the N-terminal region first amino acid position an "M” residue, an "R” residue, a “S” residue, a “H” residue, a “P” residue, a “L” residue or an “D” residue.
• a peptide or chimeric sequence peptide sequence does not have a "M” residue or an "R” residue at the first amino acid position of the N-terminal region.
• a peptide or chimeric sequence has an N-terminal region with any one of the following sequences: MDSSPL (SEQ ID NO: l 10), MSDSSPL (SEQ ID NO:
• SDSSPL SEQ ID NO: 112
• MSSPL SEQ ID NO: 113
• SSPL SEQ ID NO: 114
• a peptide or chimeric sequence has a residue at the last position of the C-terminal region that corresponds to about residue 194 of SEQ ID NO:99 [FGF19].
• a peptide sequence has or consists of any one of the following sequences:
• the R terminal residue is deleted.
• the N-terminus of the peptide sequence includes or consists of any of: HPIPDSSPLLQFGGQVRLRHLYTSG (M5-R) (amino acids 1-25 of SEQ ID NO: 160);
• RPLAFSDSSPLLQFGGQVRLRHLYTSG (amino acids 1-27 of SEQ ID NO:7);
• HPIPDSSPLLQWGDPIRLRHLYTSG (M8-R) (amino acids 2-26 of SEQ ID NO:8);
• HPIPDSSPLLQFGWGDPIRLRHLYTSG (M9-R) (amino acids 2-28 of SEQ ID NO:9);
• HPIPDSSPHVHYGWGDPIRLRHLYTSG (M10-R) (amino acids 2-28 of SEQ ID NO: 10);
• RPLAFSDAGPLLQWGDPIRLRHLYTSG (Ml 1) (amino acids 1-27 of SEQ ID NO: l 1);
• RPLAFSDAGPLLQFGWGDPIRLRHLYTSG (M12) (amino acids 1-29 of SEQ ID NO: 12);
• RPLAFSDAGPLLQFGGQVRLRHLYTSG (Ml 3) (amino acids 1-27 of SEQ ID NO: 13);
• HPIPDSSPHVHYGGQVRLRHLYTSG (M14-R) (amino acids 2-26 of SEQ ID NO: 14);
• RPLAFSDAGPHVHYGGQVRLRHLYTSG (M15) (amino acids 1-27 of SEQ ID NO: 15);
• RPLAFSDAGPHVHWGDPIRLRHLYTSG (M16) (amino acids 1-27 of SEQ ID NO: 16);
• RPLAFSDAGPHVGWGDPIRLRHLYTSG (M17) (amino acids 1-27 of SEQ ID NO: 17);
• RPLAFSDAGPHYGWGDPIRLRHLYTSG (Ml 8) (amino acids 1-27 of SEQ ID NO: 18);
• RPLAFSDAGPVYGWGDPIRLRHLYTSG (M19) (amino acids 1-27 of SEQ ID NO: 19);
• RPLAFSDAGPVHGWGDPIRLRHLYTSG (M20) (amino acids 1-27 of SEQ ID NO:20);
• RPLAFSDAGPVHYWGDPIRLRHLYTSG (M21) (amino acids 1-27 of SEQ ID NO:21);
• RPLAFSDAGPHVHGWGDPIRLRHLYTSG (M22) (amino acids 1-27 of SEQ ID NO:22);
• RPLAFSDAGPHHGWGDPIRLRHLYTSG (M23) (amino acids 1-27 of SEQ ID NO:23);
• RPLAFSDAGPHHYWGDPIRLRHLYTSG (M24) (amino acids 1-27 of SEQ ID NO:24);
• RPLAFSDAGPHVYWGDPIRLRHLYTSG (M25) (amino acids 1-27 of SEQ ID NO:25);
• RPLAFSDSSPLVHWGDPIRLRHLYTSG (M26) (amino acids 1-27 of SEQ ID NO:26);
• RPLAFSDSSPHVHWGDPIRLRHLYTSG (M27) (amino acids 1-27 of SEQ ID NO:27);
• RPLAFSDAGPHVWGDPIRLRHLYTSG (M28) (amino acids 1-26 of SEQ ID NO:28);
• RPLAFSDAGPHVHYWGDPIRLRHLYTSG (M29) (amino acids 1-28 of SEQ ID NO:29);
• RPLAFSDAGPHVHYAWGDPIRLRHLYTSG (M30) (amino acids 1-29 of SEQ ID NO:30);
• RHPIPDSSPLLQFGAQVRLRHLYTSG (M31) (amino acids 1-26 of SEQ ID NO:31);
• RHPIPDSSPLLQFGDQVRLRHLYTSG (M32) (amino acids 1-26 of SEQ ID NO:32);
• RHPIPDSSPLLQFGPQVRLRHLYTSG (M33) (amino acids 1-26 of SEQ ID NO:33);
• RHPIPDSSPLLQFGGAVRLRHLYTSG (amino acids 1-26 of SEQ ID NO:34);
• RHPIPDSSPLLQFGGEVRLRHLYTSG (M35) (amino acids 1-26 of SEQ ID NO:35);
• RHPIPDSSPLLQFGGNVRLRHLYTSG (amino acids 1-26 of SEQ ID NO:36);
• RHPIPDSSPLLQFGGQARLRHLYTSG (amino acids 1-26 of SEQ ID NO:37); RHPIPDSSPLLQFGGQIRLRHLYTSG (M38) (amino acids 1-26 of SEQ ID NO:38); RHPIPDSSPLLQFGGQTRLRHLYTSG (M39) (amino acids 1-26 of SEQ ID NO:39);
• RHPIPDSSPLLQFGWGQPVRLRHLYTSG (amino acids 1-28 of SEQ ID NO:40);
• DAGPHVHYGWGDPIRLRHLYTSG (M74-R) (amino acids 2-24 of SEQ ID NO:74);
• VHYGWGDPIRLRHLYTSG (M75-R) (amino acids 2-19 of SEQ ID NO: 75);
• RLRHLYTSG (M77-R) (amino acids 2-10 of SEQ ID NO: 77);
• RHPIPDSSPLLQFGWGDPIRLRHLYTSG (amino acids 1-28 of SEQ ID NO: 9);
• RHPIPD S SPLLQ WGDPIRLRHLYT SG (M8) (amino acids 1-26 of SEQ ID NO:8);
• RPLAF SD AGPLLQFG WGDPIRLRHLYT SG (M12) (amino acids 1-29 of SEQ ID NO: 12);
• RHPIPDSSPHVHYGWGDPIRLRHLYTSG (amino acids 1-28 of SEQ ID NO: 10);
• RPLAFSDAGPLLQFGGQVRLRHLYTSG (M13) (amino acids 1-27 of SEQ ID NO: 13);
• RHPIPDSSPHVHYGGQVRLRHLYTSG (amino acids 1-26 of SEQ ID NO: 14);
• RPLAFSDAGPHVHYGGDIRLRHLYTSG amino acids 1-27 of SEQ ID NO:43;
• RDSSPLLQFGGQVRLRHLYTSG (M6) (amino acids 1-22 of SEQ ID NO:6).
• a peptide sequence includes or consists of:
• the R terminal residue is deleted.
• a peptide sequence includes the addition of amino acid residues 30-194 of SEQ ID NO:99 [FGF19] at the C-terminus, resulting in a chimeric polypeptide.
• a peptide or chimeric sequence has an amino acid substitution, an addition, insertion or is a subsequence that has at least one amino acid deleted.
• Such amino acid substitutions, additions, insertions and deletions of a peptide sequence can be 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10 or more amino acid residues (10-20, 20-30, 30-40, 40-50, etc.), for example, at the N- or C-terminus, or internal.
• the amino acid substitution, or deletion is at any of amino acid positions 8-20 of FGF19 (AGPHVHYGWGDPI) (SEQ ID NO: 187).
• a peptide or chimeric sequence includes all or a portion of an FGF19 sequence set forth as:
• a peptide or chimeric sequence has a function or activity greater or less than a comparison sequence.
• a peptide sequence has reduced HCC formation compared to FGF19, or an FGF 19 variant sequence having any of GQV, GDI, WGPI (SEQ ID NO: 171), WGDPV (SEQ ID NO: 172), WGDI (SEQ ID NO: 173), GDPI (SEQ ID NO: 174), GPI, WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 178), WGDAI (SEQ ID NO: 179), WGDPA (SEQ ID NO: 180), WDPI (SEQ ID NO: 181), WGDI (SEQ ID NO: 182), WGDP (SEQ ID NO: 183) or FGDPI (SEQ ID NO: 184) substituted for the WGDPI (SEQ ID NO:
• a peptide or chimeric sequence has an effect on function or activity of other molecules.
• a peptide sequence maintains or increases an FGFR4 mediated activity.
• a peptide sequence binds to fibroblast growth factor receptor 4 (FGFR4) or activates FGFR4, or does not detectably bind to FGFR4 or activate FGFR4.
• FGFR4 fibroblast growth factor receptor 4
• a peptide sequence binds to FGFR4 with an affinity less than, comparable to or greater than FGF 19 binding affinity for FGFR4.
• a peptide sequence activates FGFR4 to an extent or amount less than, comparable to or greater than FGF 19 activates FGFR4.
• a peptide or chimeric sequence includes one or more L-amino acids, D-amino acids, non-naturally occurring amino acids, or amino acid mimetic, derivative or analogue.
• a peptide or chimeric sequence has an N-terminal region, or a C-terminal region, or a FGF 19 sequence portion, or an FGF21 sequence portion, joined by a linker or spacer.
• a chimeric peptide or peptide sequence is included in a pharmaceutical composition, which in turn can be used for practicing the invention methods and uses. Such compositions include combinations of inactive or other active ingredients.
• a composition, such as a pharmaceutical composition includes chimeric peptide sequence or peptide sequence and an agent that improves bile acid homeostasis.
• a use or method of treatment of a subject includes administering an invention chimeric peptide or peptide sequence to a subject, such as a subject having, or at risk of having, a disorder treatable by an invention peptide sequence, in an amount effective for treating the disorder.
• a method or use includes administering an invention chimeric peptide or peptide sequence to a subject, such as a subject having a bile acid related or associated disorder.
• a chimeric peptide sequence or peptide sequence is administered to a subject in an amount effective to improve or provide bile acid homeostasis.
• Non-limiting exemplary bile acid related or associated disorders treatable according to the invention methods and uses include: metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn
• the bile acid related or associated disorder is bile acid malabsorption. In another embodiment, the bile acid related or associated disorder is diarrhea. In another embodiment, the bile acid related or associated disorder is cholestasis (e.g., intrahepatic or extrahepatic cholestasis). In another embodiment, the bile acid related or associated disorder is primary billiary cirrhosis. In another embodiment, the bile acid related or associated disorder is primary sclerosing cholangitis. In another embodiment, the bile acid related or associated disorder is PFIC (e.g., progressive PFIC).
• PFIC progressive PFIC
• Methods and uses of analyzing and/or identifying a chimeric peptide sequence or peptide sequence are also provided, such as chimeric peptide sequences and peptide sequences that modulate bile acid homeostasis, optionally without having substantial or significant HCC activity.
• a method or use includes: a) providing a candidate peptide sequence; b) administering the candidate peptide sequence to a test animal; c) measuring bile acid levels of the animal after administration of the candidate peptide sequence, to determine if the candidate peptide sequence modulates bile acid homeostasis; and d) analyzing the candidate peptide sequence for induction of HCC in the animal, or expression of a marker correlating with HCC activity.
• a candidate peptide that modulates bile acid homeostasis but does not have substantial HCC activity thereby identifies the candidate peptide sequence as a peptide sequence having that modulates bile acid homeostasis without substantial HCC activity.
• the chimeric peptide sequence or peptide sequence is also analyzed for induction of HCC in the animal (e.g. , assessing a hepatic tissue sample from the test animal), or expression of a marker correlating with HCC activity.
• Such methods and uses identify the candidate as having bile acid homeostasis modulating activity, optionally also without substantial or significant HCC activity.
• FIG. 1 shows cyp7al expression in db/db mice dosed intraperitoneally with the indicated concentrations of FGF19 and FGF21 (SEQ ID NOs:99 and 100).
• FIG. 2A-2D show cyp7al expression in human primary hepatocytes following dosing of A) variant Ml (SEQ ID NO: l); B) variant M2 (SEQ ID NO:2); C) variant M5 (SEQ ID NO:5); and D) variant M32 (SEQ ID NO:32).
• FIG. 3A-3D show cyp7al expression in human primary hepatocytes following dosing of A) variant M69 (SEQ ID NO:69); B) variant M75 (SEQ ID NO:75); C) variant M70 (SEQ ID NO:70); and D) variant M76 (SEQ ID NO:76).
• FIG. 4A-4D show cyp7al expression in human primary hepatocytes following dosing of A) variant M85 (SEQ ID NO:85); B) variant M96 (SEQ ID NO:96); C) variant M90 (SEQ ID NO:90); and D) variant M98 (SEQ ID NO:98).
• FIG. 5 is a table showing the cyp7al IC5 0 (pM), relative cyp7al expression and HCC core of the indicated variants: Ml , M2, M5, M32, M69, M70, M75, M76, M85, M90, M96 and M98.
• FIG. 6 depicts the results of a human clinical trial, showing administration of M70 is able to suppress 7a-hydroxy-4-cholsten-3-one (C4), a marker of bile acid synthesis, as compared to a placebo.
• FIG. 7 depicts that the expression of FGFR4/p-klotho complex in L6 cells potentiates activation of intracellular signaling pathways by FGF19, M3 and M70.
• a chimeric peptide sequence includes or consists of an N-terminal region having at least seven amino acid residues and the N-terminal region having a first amino acid position and a last amino acid position, where the N-terminal region has a DSSPL (SEQ ID NO: 121) or DASPH (SEQ ID NO: 122) sequence; and a C-terminal region having a portion of FGF19 and the C-terminal region having a first amino acid position and a last amino acid position, where the C-terminal region includes amino acid residues 16-29 of FGF19 (WGDPIRLRHLYTSG; SEQ ID NO: 169) and the W residue corresponds to the first amino acid position of the C-terminal region.
• a chimeric peptide sequence includes or consists of an N- terminal region having a portion of FGF21 and the N-terminal region having a first amino acid position and a last amino acid position, where the N-terminal region has a GQV sequence and the V residue corresponds to the last amino acid position of the N-terminal region; and a C-terminal region having a portion of FGF19 and the C-terminal region having a first amino acid position and a last amino acid position where the C-terminal region includes amino acid residues 21-29 of FGF19 (RLRHLYTSG; SEQ ID NO: 185) and the R residue corresponds to the first position of the C- terminal region.
• RLRHLYTSG amino acid residues 21-29 of FGF19
• a peptide sequence includes or consists of a FGF19 sequence variant having one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF19.
• a peptide sequence includes or consists of a FGF21 sequence variant having one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF21.
• a peptide sequence includes or consists of a portion of an FGF19 sequence fused to a portion of an FGF21 sequence.
• a peptide sequence includes or consists of a portion of an FGF19 sequence fused to a portion of an FGF21 sequence, where the FGF19 and/or FGF21 sequence portion(s) have one or more amino acid substitutions, insertions or deletions compared to a reference or wild type FGF 19 and/or FGF21.
• the invention also provides methods and uses of treating a subject having or at risk of having a disorder treatable using variants and fusions of FGF19 and/or FGF21 peptide sequences.
• a method or use includes contacting or administering to a subject one or more variant or fusion FGF19 and/or FGF21 peptide sequences in an amount effective for treating a bile- acid related or associated disorder.
• a method or use includes contacting or administering to a subject one or more nucleic acid molecules encoding a variant or fusion FGF19 and/or FGF21 peptide sequence (for example, an expression control element in operable linkage with the nucleic acid encoding the peptide sequence, optionally including a vector), in an amount effective for treating a bile-acid related or associated disorder.
• a representative reference or wild type FGF19 sequence is set forth as:
• a representative reference or wild type FGF21 sequence is set forth as:
• FGF21 allelic variants include, e.g., M70, M71 and M72.
• peptide protein
• polypeptide sequence are used interchangeably herein to refer to two or more amino acids, or “residues,” including chemical modifications and derivatives of amino acids, covalently linked by an amide bond or equivalent.
• the amino acids forming all or a part of a peptide may be from among the known 21 naturally occurring amino acids, which are referred to by both their single letter abbreviation or common three-letter abbreviation.
• conventional amino acid residues have their conventional meaning. Thus, "Leu” is leucine, "He” is isoleucine, "Nle” is norleucine, and so on.
• Non-limiting particular examples are a peptide sequence with amino-terminal amino acids 1-16 of FGF21 fused to carboxy-terminal amino acids 21-194 of FGF19; a peptide sequence with amino-terminal amino acids 1-147 of FGF19 fused to carboxy-terminal amino acids
• FGF21 a peptide sequence with amino-terminal amino acids 1-20 of FGF19 fused to carboxy-terminal amino acids 17-181 of FGF21 ; a peptide sequence with amino-terminal amino acids 1-146 of FGF21 fused to carboxy-terminal amino acids 148-194 of FGF19; and a peptide sequence with amino-terminal amino acids 1-20 of FGF19 fused to internal amino acids 17-146 of FGF21 fused to carboxy-terminal amino acids 148-194 of FGF19.
• Additional particular peptides sequences have a WGDPI (SEQ ID NO: 170) sequence motif corresponding to the WGDPI sequence of amino acids 16-20 of FGF19 (SEQ ID NO:99), lack a WGDPI (SEQ ID NO: 170) sequence motif corresponding to the WGDPI sequence of amino acids 16-20 of FGF19 (SEQ ID NO: 99), or have a substituted (i.e. , mutated) WGDPI (SEQ ID NO: 170) sequence motif corresponding to FGF19 WGDPI sequence of amino acids 16-20 of FGF19 (SEQ ID NO:99).
• Particular peptide sequences of the invention also include sequences distinct from FGF19 and FGF21 (e.g. , as set forth herein), and FGF 19 variant sequences having any GQV, GDI, WGPI (SEQ ID NO: 171), WGDPV (SEQ ID NO: 172), WGDI (SEQ ID NO: 173), GDPI (SEQ ID NO: 174), GPI, WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 178), WGDAI (SEQ ID NO: 179), WGDPA (SEQ ID NO: 180), WDPI (SEQ ID NO: 181), WGDI (SEQ ID NO: 182), WGDP (SEQ ID NO: 183) or FGDPI (SEQ ID NO: 184) substituted for FGF 19 WGDPI(SEQ ID NO: 170) sequence at amino acids 16-20.
• wild-type FGF19 and FGF21 may be excluded sequences, and FGF 19 having any of GQV, GDI, WGPI(SEQ ID NO: 171),
• WGDPV (SEQ ID NO: 172), WGDI(SEQ ID NO: 173), GDPI(SEQ ID NO: 174), GPI, WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 178), WGDAI (SEQ ID NO: 179), WGDPA (SEQ ID NO: 180), WDPI (SEQ ID NO: 181), WGDI (SEQ ID NO: 182), WGDP (SEQ ID NO: 183) or FGDPI (SEQ ID NO: 184) substituted for the WGDPI(SEQ ID NO: 170) sequence at amino acids 16-20 of FGF 19 may also be excluded.
• a sequence has, for example, 3 FGF21 residues fused to FGF 19 having, for example, any of GQV, GQV, GDI, or GPI, or 2 FGF21 residues fused to any of WGPI (SEQ ID NO: 171), WGDI (SEQ ID NO: 173), GDPI (SEQ ID NO: 174), WDPI (SEQ ID NO: 181), WGDI (SEQ ID NO: 182), or WGDP (SEQ ID NO: 183).
• WGPI SEQ ID NO: 171
• WGDI SEQ ID NO: 173
• GDPI SEQ ID NO: 174
• WDPI SEQ ID NO: 181
• WGDI SEQ ID NO: 182
• WGDP SEQ ID NO: 183
• peptide sequences include or consist of all or a part of a sequence variant specified herein as M1-M98 (SEQ ID NOs: l-52, 192, and 54-98, respectively). More particular non-limiting examples of peptide sequences include or consist of all or a part of a sequence set forth as:
• peptide sequences having at the N- terminus, a peptide sequence including or consisting of all or a part of any of:
• HPIPDSSPLLQFGGQVRLRHLYTSG (M5-R) (amino acids 1-25 of SEQ ID NO: 160);
• DSSPLLQFGGQVRLRHLYTSG (M6) (M6-R) (amino acids 2-22 of SEQ ID NO:6);
• RPLAF SD S SPLLQFGGQ VRLRHLYTS G (M7) (amino acids 1-27 of SEQ ID NO:7);
• HPIPD S SPLLQ WGDPIRLRHLYT SG (M8-R) (amino acids 2-26 of SEQ ID NO:8);
• HPIPDSSPLLQFGWGDPIRLRHLYTSG (M9-R) (amino acids 2-28 of SEQ ID NO:9);
• HPIPD S SPHVHYG WGDPIRLRHLYTS G (M10-R) (amino acids 2-28 of SEQ ID NO: 10);
• RPLAF SD AGPLLQ WGDPIRLRHLYTS G (Ml 1) (amino acids 1-27 of SEQ ID NO: l 1);
• RPLAFSDAGPLLQFGWGDPIRLRHLYTSG (M12) (amino acids 1-29 of SEQ ID NO: 12);
• RPLAF SDAGPLLQFGGQ VRLRHLYTS G (M13) (amino acids 1-27 of SEQ ID NO: 13);
• HPIPD S SPHVHYGGQ VRLRHLYT SG (M14-R) (amino acids 2-26 of SEQ ID NO: 14);
• RPLAF SD AGPHVHYGGQVRLRHLYT SG (Ml 5) (amino acids 1-27 of SEQ ID NO: 15);
• RPLAF SDAGPHVH WGDPIRLRHLYT SG (Ml 6) (amino acids 1-27 of SEQ ID NO: 16);
• RPLAF SDAGPHVG WGDPIRLRHLYT SG (Ml 7) (amino acids 1-27 of SEQ ID NO: 17);
• RPLAF SDAGPHYG WGDPIRLRHLYT SG (Ml 8) (amino acids 1-27 of SEQ ID NO: 18);
• RPLAF SD AGP VYG WGDPIRLRHLYT SG (Ml 9) (amino acids 1-27 of SEQ ID NO: 19);
• RPLAF SD AGP VHG WGDPIRLRHLYT SG (M20) (amino acids 1-27 of SEQ ID NO:20);
• RPLAF SD AGP VHY WGDPIRLRHLYT SG (M21) (amino acids 1-27 of SEQ ID NO:21);
• RPLAF SDAGPHVHG WGDPIRLRHLYTS G (M22) (amino acids 1-27 of SEQ ID NO:22);
• RPLAF SDAGPHHG WGDPIRLRHLYT SG (M23) (amino acids 1-27 of SEQ ID NO:23);
• RPLAF SDAGPHHY WGDPIRLRHLYT SG (M24) (amino acids 1-27 of SEQ ID NO:24);
• RPLAF SDAGPHVY WGDPIRLRHLYT SG (M25) (amino acids 1-27 of SEQ ID NO:25);
• RPLAF SD S SPLVH WGDPIRLRHLYT SG (M26) (amino acids 1-27 of SEQ ID NO:26);
• RPLAF SDAGPHV WGDPIRLRHLYTS G (M28) (amino acids 1-26 of SEQ ID NO:28);
• RPLAFSDAGPHVHYWGDPIRLRHLYTSG (M29) (amino acids 1-28 of SEQ ID NO:29);
• RPLAFSDAGPHVHYAWGDPIRLRHLYTSG (M30) (amino acids 1-29 of SEQ ID NO:30);
• RHPIPDSSPLLQFGAQVRLRHLYTSG (M31) (amino acids 1-26 of SEQ ID NO:31);
• RHPIPDSSPLLQFGDQVRLRHLYTSG (M32) (amino acids 1-26 of SEQ ID NO:32);
• RHPIPDSSPLLQFGPQVRLRHLYTSG (M33) (amino acids 1-26 of SEQ ID NO:33);
• RHPIPDSSPLLQFGGAVRLRHLYTSG (amino acids 1-26 of SEQ ID NO:34);
• RHPIPDSSPLLQFGGEVRLRHLYTSG (M35) (amino acids 1-26 of SEQ ID NO:35); RHPIPDSSPLLQFGGNVRLRHLYTSG (M36) (amino acids 1-26 of SEQ ID NO:36); RHPIPDSSPLLQFGGQARLRHLYTSG (M37) (amino acids 1-26 of SEQ ID NO:37);
• RHPIPDSSPLLQFGGQIRLRHLYTSG (amino acids 1-26 of SEQ ID NO:38);
• RHPIPDSSPLLQFGGQTRLRHLYTSG (M39) (amino acids 1-26 of SEQ ID NO:39);
• RHPIPDSSPLLQFGWGQPVRLRHLYTSG (amino acids 1-28 of SEQ ID NO:40);
• DAGPHVHYGWGDPIRLRHLYTSG (M74-R) (amino acids 2-24 of SEQ ID NO:74);
• VHYGWGDPIRLRHLYTSG (M75-R) (amino acids 2-19 of SEQ ID NO: 75);
• RLRHLYTSG (M77-R) (amino acids 2-10 of SEQ ID NO: 77);
• RHPIPDSSPLLQFGWGDPIRLRHLYTSG (amino acids 1-28 of SEQ ID NO: 9);
• RHPIPD S SPLLQ WGDPIRLRHLYT SG (M8) (amino acids 1-26 of SEQ ID NO:8);
• RPLAF SD AGPLLQFG WGDPIRLRHLYT SG (M12) (amino acids 1-29 of SEQ ID NO: 12);
• RHPIPDSSPHVHYGWGDPIRLRHLYTSG (amino acids 1-28 of SEQ ID NO: 10);
• RPLAFSDAGPLLQFGGQVRLRHLYTSG (M13) (amino acids 1-27 of SEQ ID NO: 13);
• RHPIPDSSPHVHYGGQVRLRHLYTSG (amino acids 1-26 of SEQ ID NO: 14);
• RPLAFSDAGPHVHYGGDIRLRHLYTSG amino acids 1-27 of SEQ ID NO:43;
• RDSSPLLQFGGQVRLRHLYTSG (M6) (amino acids 1-22 of SEQ ID NO:6);
• amino terminal R residue may be deleted.
• Peptide sequences of the invention additionally include those with reduced or absent induction or formation of HCC compared to FGF 19, or an FGF 19 variant sequence having any of
• GQV GQV, GDI, WGPI (SEQ ID NO: 171), WGDPV (SEQ ID NO: 172), WGDI (SEQ ID NO: 173), GDPI
• WDPI SEQ ID NO: 181
• WGDI SEQ ID NO: 182
• WGDP SEQ ID NO: 183
• FGDPI FGDPI
• Peptide sequences of the invention also include those with greater glucose lowering activity compared to FGF 19, or an FGF 19 variant sequence having any of GQV, GDI, WGPI, WGPI (SEQ ID NO: 1).
• WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WA
• WGDI (SEQ ID NO: 182), WGDP (SEQ ID NO: 183) or FGDPI (SEQ ID NO: 184) substituted for the WGDPI (SEQ ID NO: 170) sequence at amino acids 16-20 of FGF19.
• Peptide sequences of the invention moreover include those with less lipid (e.g., triglyceride, cholesterol, non-HDL or HDL) increasing activity compared to FGF 19, or an FGF 19 variant sequence having any of GQV, GDI, WGPI (SEQ ID NO: 171), WGDPV (SEQ ID NO: 172), WGDI (SEQ ID NO: 173), GDPI (SEQ ID NO: 174), GPI, WGQPI (SEQ ID NO: 175), WGAPI (SEQ ID NO: 176), AGDPI (SEQ ID NO: 177), WADPI (SEQ ID NO: 178), WGDAI (SEQ ID NO: 179), WGDPA (SEQ ID NO: 180), WD
• the number of amino acids or residues in an invention peptide sequence will total less than about 250 (e.g., amino acids or mimetics thereof).
• the number of residues comprise from about 20 up to about 200 residues (e.g., amino acids or mimetics thereof).
• the number of residues comprise from about 50 up to about 200 residues (e.g., amino acids or mimetics thereof).
• the number of residues comprise from about 100 up to about 195 residues (e.g., amino acids or mimetics thereof) in length.
• Amino acids or residues can be linked by amide or by non-natural and non-amide chemical bonds including, for example, those formed with glutaraldehyde, N-hydroxysuccinimide esters, bifunctional maleimides, or N, N'-dicyclohexylcarbodiimide (DCC).
• Non-amide bonds include, for example, ketomethylene, aminomethylene, olefin, ether, thioether and the like (see, e.g., Spatola in Chemistry and Biochemistry of Amino Acids, Peptides and Proteins, Vol. 7, pp 267-357 (1983), "Peptide and Backbone Modifications," Marcel Decker, NY).
• a peptide of the invention when a peptide of the invention includes a portion of an FGF19 sequence and a portion of an FGF21 sequence, the two portions need not be joined to each other by an amide bond, but can be joined by any other chemical moiety or conjugated together via a linker moiety.
• the invention also includes subsequences, variants and modified forms of the exemplified peptide sequences (including the FGF19 and FGF21 variants and subsequences listed in Tables 1-10 and Sequence Listing), so long as the foregoing retains at least a detectable or measureable activity or function.
• certain exemplified variant peptides have FGF19 C- terminal sequence
• EPEDLRGHLESDMFSSPLETDSMDPFGLVTGLEAVRSPSFEK (SEQ ID NO: 188) at the C- terminal portion, e.g., following the "TSG" amino acid residues of the variant.
• FGF21 sequence at the amino-terminus have an "R” residue positioned at the N-terminus, which can be omitted.
• certain exemplified variant peptides include an "M” residue positioned at the N-terminus, which can be appended to or further substituted for an omitted residue, such as an "R” residue.
• peptide sequences at the N-terminus include any of: RDSS (SEQ ID NO: 1 15), DSS, MDSS (SEQ ID NO: 1 16) or MRDSS (SEQ ID NO: 1 17).
• peptide sequences include those with the following residues at the N- terminus: MDSSPL (SEQ ID NO: l 19), MSDSSPL (SEQ ID NO: 120) (cleaved to SDSSPL(SEQ ID NO: 1 12)) and MSSPL (SEQ ID NO: 1 13) (cleaved to SSPL (SEQ ID NO: 1 14)).
• the "peptide,” “polypeptide,” and “protein” sequences of the invention include subsequences, variants and modified forms of the FGF19 and FGF21 variants and subsequences listed in Tables 1-10 and Sequence Listing, and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and Sequence Listing, so long as the subsequence, variant or modified form (e.g. , fusion or chimera) retains at least a detectable activity or function, e.g. , modulates bile acid homeostasis.
• the subsequence, variant or modified form e.g. , fusion or chimera
• modified peptide sequences, nucleic acids and other compositions may have greater or less activity or function, or have a distinct function or activity compared with a reference unmodified peptide sequence, nucleic acid, or other composition, or may have a property desirable in a protein formulated for therapy (e.g. serum half-life), to elicit antibody for use in a detection assay, and/or for protein purification.
• a peptide sequence of the invention can be modified to increase serum half-life, to increase in vitro and/or in vivo stability of the protein, etc.
• Such subsequences, variants and modified forms of the peptide sequences exemplified herein include substitutions, deletions and/or insertions/additions of one or more amino acids, to or from the amino terminus, the carboxy-terminus or internally.
• substitutions, deletions and/or insertions/additions of one or more amino acids include substitutions, deletions and/or insertions/additions of one or more amino acids, to or from the amino terminus, the carboxy-terminus or internally.
• One example is a substitution of an amino acid residue for another amino acid residue within the peptide sequence.
• Another is a deletion of one or more amino acid residues from the peptide sequence, or an insertion or addition of one or more amino acid residues into the peptide sequence.
• the number of residues substituted, deleted or inserted/added are one or more amino acids (e.g. , 1-3, 3-5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60-70, 70-80, 80-90, 90-100, 100-1 10,
• an FGF19 or FGF21 sequence can have few or many amino acids substituted, deleted or inserted/added (e.g. , 1-3, 3-5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60-70, 70-80, 80-90, 90-100, 100-1 10, 110-120, 120-130, 130-140, 140-150, 150-160, 160- 170, 170-180, 180-190, 190-200, 200-225, 225-250, or more).
• an FGF 19 amino acid sequence can include or consist of an amino acid sequence of about 1-3, 3-5, 5-10, 10-20, 20-30, 30- 40, 40-50, 50-60, 60-70, 70-80, 80-90, 90-100, 100-1 10, 1 10-120, 120-130, 130-140, 140-150, 150- 160, 160-170, 170-180, 180-190, 190-200, 200-225, 225-250, or more amino acids from FGF21 ; or an FGF21 amino acid or sequence can include or consist of an amino acid sequence of about 1-3, 3- 5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60-70, 70-80, 80-90, 90-100, 100-1 10, 1 10-120, 120-130, 130-140, 140-150, 150-160, 160-170, 170-180, 180-190, 190-200, 200-225, 225-250, or more amino acids from FGF 19.
• substitutions include substituting a D residue for an L-residue. Accordingly, although residues are listed in the L-isomer configuration D-amino acids at any particular or all positions of the peptide sequences of the invention are included, unless a D-isomer leads to a sequence that has no detectable or measurable function.
• a "conservative substitution” is a replacement of one amino acid by a biologically, chemically or structurally similar residue.
• Biologically similar means that the substitution is compatible with a biological activity, e.g. , glucose lowering activity.
• Structurally similar means that the amino acids have side chains with similar length, such as alanine, glycine and serine, or having similar size, or the structure of a first, second or additional peptide sequence is maintained.
• Chemical similarity means that the residues have the same charge or are both hydrophilic and hydrophobic.
• Particular examples include the substitution of one hydrophobic residue, such as isoleucine, valine, leucine or methionine for another, or the substitution of one polar residue for another, such as the substitution of arginine for lysine, glutamic for aspartic acids, or glutamine for asparagine, serine for threonine, etc.
• Routine assays can be used to determine whether a subsequence, variant or modified form has activity, e.g. , glucose lowering activity.
• a reference peptide sequence for example, a peptide sequence in any of
• a BLASTP algorithm is typically used in combination with a scoring matrix, such as PAM100, PAM 250, BLOSUM 62 or BLOSUM 50.
• FASTA e.g. , FASTA2 and FASTA3
• SSEARCH sequence comparison programs are also used to quantitate the extent of identity (Pearson et al, Proc. Natl. Acad. Sci. USA 85:2444 (1988); Pearson, Methods Mol Biol. 132: 185 (2000); and Smith et al., J. Mol. Biol. 147: 195 (1981)).
• Programs for quantitating protein structural similarity using Delaunay-based topological mapping have also been developed (Bostick et al., Biochem Biophys Res Commun. 304:320 (2003)).
• peptide sequences including subsequences, variants and modified forms of the peptide sequences exemplified herein (e.g. , sequences listed in Tables 1-10 and Sequence
• an "amino acid” or “residue” includes conventional alpha-amino acids as well as beta-amino acids, alpha, alpha disubstituted amino acids and N-substituted amino acids wherein at least one side chain is an amino acid side chain moiety as defined herein.
• An "amino acid” further includes N- alkyl alpha-amino acids, wherein the N-terminus amino group has a Ci to e linear or branched alkyl substituent.
• the term “amino acid” therefore includes stereoisomers and modifications of naturally occurring protein amino acids, non-protein amino acids, post-translationally modified amino acids
• amino acid side chain moiety as used herein includes any side chain of any amino acid, as the term “amino acid” is defined herein. This therefore includes the side chain moiety in naturally occurring amino acids. It further includes side chain moieties in modified naturally occurring amino acids as set forth herein and known to one of skill in the art, such as side chain moieties in stereoisomers and modifications of naturally occurring protein amino acids, non-protein amino acids, post- translationally modified amino acids, enzymatically modified or synthesized amino acids, derivatized amino acids, constructs or structures designed to mimic amino acids, etc. For example, the side chain moiety of any amino acid disclosed herein or known to one of skill in the art is included within the definition.
• a "derivative of an amino acid side chain moiety" is included within the definition of an amino acid side chain moiety.
• suitable protecting groups are known to the skilled artisan. Provided such derivatization provides a desired activity in the final peptide sequence ⁇ e.g., glucose lowering, improved glucose or lipid metabolism, anti-diabetic activity, absence of substantial HCC formation or tumorigenesis, absence of substantial modulation of lean or fat mass, etc).
• amino acid side chain moiety includes all such derivatization, and particular non- limiting examples include: gamma-amino butyric acid, 12-amino dodecanoic acid, alpha- aminoisobutyric acid, 6-amino hexanoic acid, 4-(aminomethyl)-cyclohexane carboxylic acid, 8- amino octanoic acid, biphenylalanine, Boc ⁇ t-butoxycarbonyl, benzyl, benzoyl, citrulline, diaminobutyric acid, pyrrollysine, diaminopropionic acid, 3,3-diphenylalanine, orthonine, citrulline, l,3-dihydro-2H-isoindolecarboxylic acid, ethyl, Fmoc— fiuorenylmethoxycarbonyl, heptanoyl (CH 3 - -(CH 2 ) 5 ⁇ C
• a single amino acid including stereoisomers and modifications of naturally occurring protein amino acids, non-protein amino acids, post-translationally modified amino acids,
• enzymatically synthesized amino acids non-naturally occurring amino acids including derivatized amino acids, an alpha, alpha disubstituted amino acid derived from any of the foregoing (i.e. , an alpha, alpha disubstituted amino acid, wherein at least one side chain is the same as that of the residue from which it is derived), a beta-amino acid derived from any of the foregoing (i.e.
• a beta- amino acid which other than for the presence of a beta-carbon is otherwise the same as the residue from which it is derived) etc., including all of the foregoing can be referred to herein as a "residue.”
• Suitable substituents, in addition to the side chain moiety of the alpha-amino acid, include CI to C6 linear or branched alkyl.
• Aib is an example of an alpha, alpha disubstituted amino acid.
• alpha, alpha disubstituted amino acids can be referred to using conventional L- and D-isomeric references, it is to be understood that such references are for convenience, and that where the substituents at the alpha-position are different, such amino acid can interchangeably be referred to as an alpha, alpha disubstituted amino acid derived from the L- or D-isomer, as appropriate, of a residue with the designated amino acid side chain moiety.
• (S)-2-Amino-2-methyl-hexanoic acid can be referred to as either an alpha, alpha disubstituted amino acid derived from L-Nle (norleucine) or as an alpha, alpha disubstituted amino acid derived from D-Ala.
• Aib can be referred to as an alpha, alpha disubstituted amino acid derived from Ala. Whenever an alpha, alpha disubstituted amino acid is provided, it is to be understood as including all (R) and (S) configurations thereof.
• N-substituted amino acid includes any amino acid wherein an amino acid side chain moiety is covalently bonded to the backbone amino group, optionally where there are no substituents other than H in the alpha-carbon position.
• Sarcosine is an example of an N-substituted amino acid.
• sarcosine can be referred to as an N-substituted amino acid derivative of Ala, in that the amino acid side chain moiety of sarcosine and Ala is the same, i.e. , methyl.
• Covalent modifications of the invention peptide sequences, including subsequences, variants and modified forms of the peptide sequences exemplified herein (e.g., sequences listed in Tables 1-10 and Sequence Listing), are included in the invention.
• One type of covalent modification includes reacting targeted amino acid residues with an organic derivatizing agent that is capable of reacting with selected side chains or the N- or C-terminal residues of the peptide.
• Derivatization with bifunctional agents is useful, for instance, for cross linking peptide to a water-insoluble support matrix or surface for use in the method for purifying anti-peptide antibodies, and vice-versa.
• cross linking agents include, e.g. , 1 , 1 -bis(diazoacetyl)-2-phenylethane,
• N-hydroxysuccinimide esters for example, esters with 4-azidosalicylic acid, homobifunctional imidoesters, including disuccinimidyl esters such as 3,3'- dithiobis(succinimidylpropionate), bifunctional maleimides such as bis-N-maleimido-l ,8-octane and agents such as methyl-3-[(p-azidophenyl)dithio]propioimidate.
• peptidomimetic includes a molecule that is a mimic of a residue (referred to as a "mimetic"), including but not limited to piperazine core molecules, keto-piperazine core molecules and diazepine core molecules.
• an amino acid mimetic of an invention peptide sequence includes both a carboxyl group and amino group, and a group corresponding to an amino acid side chain, or in the case of a mimetic of Glycine, no side chain other than hydrogen.
• these would include compounds that mimic the sterics, surface charge distribution, polarity, etc. of a naturally occurring amino acid, but need not be an amino acid, which would impart stability in the biological system.
• Proline may be substituted by other lactams or lactones of suitable size and substitution;
• Leucine may be substituted by an alkyl ketone, N-substituted amide, as well as variations in amino acid side chain length using alkyl, alkenyl or other substituents, others may be apparent to the skilled artisan.
• the essential element of making such substitutions is to provide a molecule of roughly the same size and charge and configuration as the residue used to design the molecule. Refinement of these modifications will be made by analyzing the compounds in a functional ⁇ e.g., glucose lowering) or other assay, and comparing the structure activity relationship. Such methods are within the scope of the skilled artisan working in medicinal chemistry and drug development.
• glycosylation broadly refers to the presence, addition or attachment of one or more sugar ⁇ e.g., carbohydrate) moieties to proteins, lipids or other organic molecules.
• deglycosylation herein is generally intended to mean the removal or deletion, of one or more sugar ⁇ e.g. , carbohydrate) moieties.
• the phrase includes qualitative changes in the glycosylation of the native proteins involving a change in the type and proportions (amount) of the various sugar (e.g., carbohydrate) moieties present.
• Glycosylation can be achieved by modification of an amino acid residue, or by adding one or more glycosylation sites that may or may not be present in the native sequence.
• a typically non-glycosylated residue can be substituted for a residue that may be glycosylated.
• Addition of glycosylation sites can be accomplished by altering the amino acid sequence.
• the alteration to the peptide sequence may be made, for example, by the addition of, or substitution by, one or more serine or threonine residues (for O-linked glycosylation sites) or asparagine residues (for N-linked glycosylation sites).
• the structures of N-linked and O-linked oligosaccharides and the sugar residues found in each type may be different.
• One type of sugar that is commonly found on both is N-acetylneuraminic acid (hereafter referred to as sialic acid).
• sialic acid is usually the terminal residue of both N-linked and O-linked oligosaccharides and, by virtue of its negative charge, may confer acidic properties to the glycoprotein.
• Peptide sequences of the invention may optionally be altered through changes at the nucleotide (e.g., DNA) level, particularly by mutating the DNA encoding the peptide at preselected bases such that codons are generated that will translate into the desired amino acids.
• Another means of increasing the number of carbohydrate moieties on the peptide is by chemical or enzymatic coupling of glycosides to the polypeptide (see, for example, in WO 87/05330).
• De-glycosylation can be accomplished by removing the underlying glycosylation site, by deleting the glycosylation by chemical and/or enzymatic means, or by substitution of codons encoding amino acid residues that are glycosylated.
• Chemical deglycosylation techniques are known, and enzymatic cleavage of carbohydrate moieties on polypeptides can be achieved by the use of a variety of endo- and exo- glycosidases.
• DHFR Dihydrofolate reductase
• CHO Chinese Hamster Ovary
• Another type of modification is to conjugate (e.g., link) one or more additional components or molecules at the N- and/or C-terminus of an invention peptide sequence, such as another protein (e.g., a protein having an amino acid sequence heterologous to the subject protein), or a carrier molecule.
• an exemplary peptide sequence can be a conjugate with another component or molecule.
• the amino- or carboxy- terminus of an invention peptide sequence can be fused with an immunoglobulin Fc region (e.g., human Fc) to form a fusion conjugate (or fusion molecule).
• Fc fusion conjugates can increase the systemic half-life of biopharmaceuticals, and thus the biopharmaceutical product may have prolonged activity or require less frequent administration.
• Fc binds to the neonatal Fc receptor (FcRn) in endothelial cells that line the blood vessels, and, upon binding, the Fc fusion molecule is protected from degradation and re- released into the circulation, keeping the molecule in circulation longer.
• Fc binding is believed to be the mechanism by which endogenous IgG retains its long plasma half-life.
• Well-known and validated Fc-fusion drugs consist of two copies of a biopharmaceutical linked to the Fc region of an antibody to improve pharmacokinetics, solubility, and production efficiency. More recent Fc-fusion technology links a single copy of a biopharmaceutical to Fc region of an antibody to optimize the pharmacokinetic and pharmacodynamic properties of the biopharmaceutical as compared to traditional Fc-fusion conjugates.
• a conjugate modification can be used to produce a peptide sequence that retains activity with an additional or complementary function or activity of the second molecule.
• a peptide sequence may be conjugated to a molecule, e.g., to facilitate solubility, storage, in vivo or shelf half- life or stability, reduction in immunogenicity, delayed or controlled release in vivo, etc.
• Other functions or activities include a conjugate that reduces toxicity relative to an unconjugated peptide sequence, a conjugate that targets a type of cell or organ more efficiently than an
• unconjugated peptide sequence or a drug to further counter the causes or effects associated with a disorder or disease as set forth herein (e.g., diabetes).
• a disorder or disease e.g., diabetes
• polyoxyalkylenes can prolong half-life.
• a linking moiety covalently bound to both the protein and the nonproteinaceous polymer e.g., a PEG
• PEG-conjugated biomolecules have been shown to possess clinically useful properties, including better physical and thermal stability, protection against susceptibility to enzymatic degradation, increased solubility, longer in vivo circulating half-life and decreased clearance, reduced immunogenicity and
• PEGs suitable for conjugation to an invention peptide sequence is generally soluble in water at room temperature, and have the general formula R(0-CH 2 -CH 2 ) n O-R, where R is hydrogen or a protective group such as an alkyl or an alkanol group, and where n is an integer from 1 to 1000. When R is a protective group, it generally has from 1 to 8 carbons.
• the PEG conjugated to the peptide sequence can be linear or branched. Branched PEG derivatives, "star-PEGs" and multi- armed PEGs are included in the invention.
• a molecular weight of the PEG used in the invention is not restricted to any particular range, but certain embodiments have a molecular weight between 500 and 20,000 while other embodiments have a molecular weight between 4,000 and 10,000.
• the invention includes compositions of conjugates wherein the PEGs have different "n" values and thus the various different PEGs are present in specific ratios.
• Such compositions can be produced by reaction conditions and purification methods know in the art.
• PEG may directly or indirectly (e.g. , through an intermediate) bind to the peptide sequences of the invention.
• PEG binds via a terminal reactive group (a "spacer").
• the spacer is, for example, a terminal reactive group which mediates a bond between the free amino or carboxyl groups of one or more of the peptide sequences and polyethylene glycol.
• the PEG having the spacer which may be bound to the free amino group includes N- hydroxysuccinylimide polyethylene glycol which may be prepared by activating succinic acid ester of polyethylene glycol with N-hydroxysuccinylimide.
• Another activated polyethylene glycol which may be bound to free amino group is 2,4-bis(0-methoxypolyethyleneglycol)-6-chloro-s-triazine which may be prepared by reacting polyethylene glycol monomethyl ether with cyanuric chloride.
• the activated polyethylene glycol which is bound to the free carboxyl group includes
• Conjugation of one or more of invention peptide sequences to PEG having a spacer may be carried out by various conventional methods.
• the conjugation reaction can be carried out in solution at a pH of from 5 to 10, at temperature from 4°C to room temperature, for 30 minutes to 20 hours, utilizing a molar ratio of reagent to protein of from 4: 1 to 30: 1.
• Various methods known in the art may be used to terminate the reaction. In some embodiments the reaction is terminated by acidifying the reaction mixture and freezing at, e.g., -20°C.
• invention peptide sequences including subsequences, sequence variants and modified forms of the exemplified peptide sequences (including the peptides listed in Tables 1-10 and
• Sequence Listing further include conjugation to large, slowly metabolized macromolecules such as proteins; polysaccharides, such as sepharose, agarose, cellulose, cellulose beads; polymeric amino acids such as polyglutamic acid, polylysine; amino acid copolymers; inactivated virus particles; inactivated bacterial toxins such as toxoid from diphtheria, tetanus, cholera, leukotoxin molecules; inactivated bacteria; and dendritic cells.
• conjugated forms if desired, can be used to produce antibodies against peptide sequences of the invention.
• Additional suitable components and molecules for conjugation include, for example, thyroglobulin; albumins such as human serum albumin (HSA); tetanus toxoid; Diphtheria toxoid; polyamino acids such as poly(D-lysine:D-glutamic acid); VP6 polypeptides of rotaviruses; influenza virus hemagglutinin, influenza virus nucleoprotein; Keyhole Limpet Hemocyanin (KLH); and hepatitis B virus core protein and surface antigen; or any combination of the foregoing.
• HSA human serum albumin
• tetanus toxoid diphtheria toxoid
• polyamino acids such as poly(D-lysine:D-glutamic acid)
• VP6 polypeptides of rotaviruses influenza virus hemagglutinin, influenza virus nucleoprotein
• KLH Keyhole Limpet Hemocyanin
• Fusion of albumin to an invention peptide sequence can, for example, be achieved by genetic manipulation, such that the DNA coding for HSA (human serum albumin), or a fragment thereof, is joined to the DNA coding for a peptide sequence. Thereafter, a suitable host can be transformed or transfected with the fused nucleotide sequence in the form of, for example, a suitable plasmid, so as to express a fusion polypeptide.
• the expression may be effected in vitro from, for example, prokaryotic or eukaryotic cells, or in vivo from, for example, a transgenic organism.
• the expression of the fusion protein is performed in mammalian cell lines, for example, CHO cell lines.
• Albuferon® Human Genome Sciences
• albumin a combination of albumin and interferon a-2B used to treat hepatitis C infection.
• dAbs are the smallest functional binding units of human antibodies (IgGs) and have favorable stability and solubility characteristics.
• the technology entails a dAb(s) conjugated to HSA (thereby forming a "AlbudAb”; see, e.g., EP1517921B, WO2005/118642 and WO2006/051288) and a molecule of interest (e.g., a peptide sequence of the invention).
• AlbudAbs are often smaller and easier to manufacture in microbial expression systems, such as bacteria or yeast, than current technologies used for extending the serum half-life of peptides. As HSA has a half-life of about three weeks, the resulting conjugated molecule improves the half-life.
• Use of the dAb technology may also enhance the efficacy of the molecule of interest.
• Additional suitable components and molecules for conjugation include those suitable for isolation or purification.
• Particular non-limiting examples include binding molecules, such as biotin (biotin-avidin specific binding pair), an antibody, a receptor, a ligand, a lectin, or molecules that comprise a solid support, including, for example, plastic or polystyrene beads, plates or beads, magnetic beads, test strips, and membranes.
• Purification methods such as cation exchange chromatography may be used to separate conjugates by charge difference, which effectively separates conjugates into their various molecular weights.
• the cation exchange column can be loaded and then washed with -20 mM sodium acetate, pH -4, and then eluted with a linear (0 M to 0.5 M) NaCl gradient buffered at a pH from 3 to 5.5, preferably at pH -4.5.
• the content of the fractions obtained by cation exchange chromatography may be identified by molecular weight using conventional methods, for example, mass spectroscopy, SDS-PAGE, or other known methods for separating molecular entities by molecular weight.
• a fraction is then accordingly identified which contains the conjugate having the desired number of PEGs attached, purified free from unmodified protein sequences and from conjugates having other numbers of PEGs attached.
• an invention peptide sequence is linked to a chemical agent (e.g., an immunotoxin or chemotherapeutic agent), including, but are not limited to, a cytotoxic agent, including taxol, cytochalasin B, gramicidin D, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, and analogs or homologs thereof.
• a chemical agent e.g., an immunotoxin or chemotherapeutic agent
• a cytotoxic agent including taxol, cytochalasin B, gramicidin D, mitomycin, etoposide, tenoposide, vincristine, vinblastine, colchicin, doxorubicin, daunorubicin, and analogs or homologs thereof.
• Other chemical agents include, for example, antimetabolites (e.g., methotrexate, 6-mercaptopurine, 6- thioguanine, cytarabine, 5-fluorouracil decarbazine); alkylating agents (e.g., mechlorethamine, carmustine and lomustine, cyclothosphamide, busulfan, dibromomannitol, streptozotocin, mitomycin C, and cisplatin); antibiotics (e.g., bleomycin); and anti-mitotic agents (e.g., vincristine and vinblastine).
• Cytotoxins can be conjugated to a peptide of the invention using linker technology known in the art and described herein.
• suitable components and molecules for conjugation include those suitable for detection in an assay.
• detectable labels such as a radioisotope (e.g. , I; S, P; P), an enzyme which generates a detectable product (e.g., luciferase, ⁇ -galactosidase, horse radish peroxidase and alkaline phosphatase), a fluorescent protein, a chromogenic protein, dye (e.g., fluorescein isothiocyanate); fluorescence emitting metals (e.g., 152 Eu); chemiluminescent compounds (e.g., luminol and acridinium salts); bioluminescent compounds (e.g., luciferin); and fluorescent proteins.
• Indirect labels include labeled or detectable antibodies that bind to a peptide sequence, where the antibody may be detected.
• a peptide sequence of the invention is conjugated to a radioactive isotope to generate a cytotoxic radiopharmaceutical (radioimmunoconjugates) useful as a diagnostic or therapeutic agent.
• radioactive isotopes include, but are not limited to, iodine 13 indium 111 , yttrium 90 and lutetium 177 .
• Methods for preparing radioimmunoconjugates are known to the skilled artisan. Examples of radioimmunoconjugates that are commercially available include ibritumomab, tiuxetan, and tositumomab.
• Suitable linkers include "flexible linkers" which are generally of sufficient length to permit some movement between the modified peptide sequences and the linked components and molecules.
• the linker molecules are generally about 6-50 atoms long.
• the linker molecules may also be, for example, aryl acetylene, ethylene glycol oligomers containing 2-10 monomer units, diamines, diacids, amino acids, or combinations thereof.
• Suitable linkers can be readily selected and can be of any suitable length, such as 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 10-20, 20-30, 30-50 amino acids (e.g., Gly).
• Exemplary flexible linkers include glycine polymers (G)n, glycine-serine polymers (for example, (GS) n , GSGGS n (SEQ ID NO: 129) and GGGS n (SEQ ID NO: 130), where n is an integer of at least one), glycine-alanine polymers, alanine-serine polymers, and other flexible linkers.
• Glycine and glycine-serine polymers are relatively unstructured, and therefore may serve as a neutral tether between components.
• Exemplary flexible linkers include, but are not limited to GGSG (SEQ ID NO: 131), GGSGG (SEQ ID NO: 132), GSGSG (SEQ ID NO: 133), GSGGG (SEQ ID NO: 134), GGGSG (SEQ ID NO: 189), and GSSSG (SEQ ID NO: 135).
• Peptide sequences of the invention including the FGF19 and FGF21 variants and subsequences and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and Sequence
• Tables 1-10 and Sequence Listing have one or more activities as set forth herein.
• One example of an activity is modulating bile acid homeostasis.
• Another example of an activity is reduced stimulation or formation of HCC, for example, as compared to FGF19.
• An additional example of an activity is lower or reduced lipid (e.g., triglyceride, cholesterol, non-HDL) or HDL increasing activity, for example, as compared to FGF21.
• a further example of an activity is a lower or reduced lean muscle mass reducing activity, for example, as compared to FGF21.
• an activity is binding to FGFR4, or activating FGFR4, for example, peptide sequences that bind to FGFR4 with an affinity comparable to or greater than FGF 19 binding affinity for FGFR4; and peptide sequences that activate FGFR4 to an extent or amount comparable to or greater than FGF 19 activates FGFR4.
• activities include treating a bile-acid related or associated disorder.
• peptide sequences of the invention including the FGF19 and FGF21 variants and subsequences and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and
• Tables 1-10 and Sequence Listing include those with the following activities: peptide sequences modulating bile acid homeostasis or treating a bile-acid related or associated disorder while having reduced HCC formation compared to FGF 19, or an FGF 19 variant sequence having any of GQV,
• GDI GDI
• WGPI SEQ ID NO: 171
• WGDPV SEQ ID NO: 172
• WGDI SEQ ID NO: 173
• GDPI SEQ ID NO: 1
• WDPI SEQ ID NO: 181
• WGDI SEQ ID NO: 182
• WGDP SEQ ID NO: 183
• FGDPI FGDPI
• WGDI SEQ ID NO: 173
• GDPI SEQ ID NO: 174
• GPI GPI
• WGQPI SEQ ID NO: 175
• WGAPI SEQ ID NO: 173
• WGDPA SEQ ID NO: 180
• WDPI SEQ ID NO: 181
• WGDI SEQ ID NO: 182
• WGDP SEQ ID NO: 169
• FGDPI substituted for the WGDPI (SEQ ID NO: 170) sequence at amino acids 16-20 of FGF 19; peptide sequences having less lipid increasing activity (e.g., less triglyceride, cholesterol, non-HDL) or more HDL increasing activity compared to FGF 19, or an FGF
• peptide sequences of the invention including the FGF19 and FGF21 variants and subsequences and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and Sequence Listing, as well as subsequences, variants and modified forms of the sequences listed in Tables 1-10 and the Sequence Listing include those with the following activities: peptide sequences that modulate bile acid homeostasis; peptide sequences that treat a bile-acid related or associated disorder, peptide sequences that bind to FGFR4, or activate FGFR4, such as peptide sequences that bind to FGFR4 with an affinity comparable to or greater than FGF19 binding affinity for FGFR4; peptide sequences that activate FGFR4 to an extent or amount comparable to or greater than FGF19 activates FGFR4; peptide sequences that down-regulate or reduce aldo-keto reductase gene expression, for example, compared to F
• variants include various N-terminal modifications and/or truncations of FGF19, including variants in which there has been a substitution of one or several N-terminal FGF19 amino acids with amino acids from FGF21.
• variants include variants having glucose lowering activity, as well as a favorable lipid profile and are not measurably or detectably tumorigenic.
• Loop-8 region are disclosed herein that have glucose lowering activity and also possess favorable metabolic parameters without exhibiting substantial
• FGF19 residues 127-129 are defined as constituting the Loop-8 region, although in the literature the Loop-8 region is sometimes defined as including or consisting of other residues (e.g., residues 125-129).
• certain combinations of R127L and P128E substitutions to the FGF19 framework had an unexpectedly positive effect on HCC formation.
• a combination of R127L and P128E substitutions and a substitution of Gin (Q) for Leu (L) in the FGF19 core region had an even more significant effect on preventing HCC formation.
• variants of FGF19 Loop-8 region are included since they can reduce or eliminate substantial, measurable or detectable HCC formation. Furthermore, the effect of reducing HCC formation may be enhanced by modifications to amino acid residues outside of the Loop 8 region (e.g., substitutions of amino acid residues in the core region).
• Activities such as, for example, modulation of bile acid homeostasis, glucose lowering activity, analysis of a bile-acid related or associated disorder, HCC formation or tumorigenesis, lipid increasing activity, or lean mass reducing activity can be ascertained in an animal, such as a db/db mouse. Measurement of binding to FGFR4 or activation of FGFR4 can be ascertained by assays disclosed herein or known to the skilled artisan.
• binding when used in reference to a peptide sequence, means that the peptide sequence interacts at the molecular level.
• a peptide sequence that binds to FGFR4 binds to all or a part of the FGFR4 sequence.
• Specific and selective binding can be distinguished from non-specific binding using assays known in the art (e.g., competition binding,
• Peptides and peptidomimetics can be produced and isolated using methods known in the art. Peptides can be synthesized, in whole or in part, using chemical methods (see, e.g., Caruthers (1980). Nucleic Acids Res. Symp. Ser. 215; Horn (1980); and Banga, A.K., Therapeutic Peptides and Proteins, Formulation, Processing and Delivery Systems (1995) Technomic Publishing Co.,
• Peptide synthesis can be performed using various solid-phase techniques (see, e.g., Roberge Science 269:202 (1995); Merrifield, Methods Enzymol. 289:3 (1997)) and automated synthesis may be achieved, e.g. , using the ABI 431 A Peptide Synthesizer (Perkin Elmer) in accordance with the manufacturer's instructions. Peptides and peptide mimetics can also be synthesized using combinatorial methodologies. Synthetic residues and polypeptides incorporating mimetics can be synthesized using a variety of procedures and methodologies known in the art (see, e.g.. Organic Syntheses Collective Volumes, Gilman, et al.
• Modified peptides can be produced by chemical modification methods (see, for example, Belousov, Nucleic Acids Res. 25:3440 (1997); Frenkel, Free Radic. Biol. Med. 19:373 (1995); and Blommers, Biochemistry 33:7886 (1994)). Peptide sequence variations, derivatives, substitutions and modifications can also be made using methods such as oligonucleotide-mediated (site-directed) mutagenesis, alanine scanning, and PCR based mutagenesis. Site-directed mutagenesis (Carter et al., Nucl.
• a "synthesized” or “manufactured” peptide sequence is a peptide made by any method involving manipulation by the hand of man. Such methods include but are not limited to the aforementioned, such as chemical synthesis, recombinant DNA technology, biochemical or enzymatic fragmentation of larger molecules, and combinations of the foregoing.
• Peptide sequences of the invention including subsequences, sequence variants and modified forms of the exemplified peptide sequences (e.g. , sequences listed in Tables 1-10 and the Sequence Listing), can also be modified to form a chimeric molecule.
• peptide sequences that include a heterologous domain. Such domains can be added to the amino-terminus or at the carboxyl-terminus of the peptide sequence.
• Heterologous domains can also be positioned within the peptide sequence, and/or alternatively flanked by FGF19 and/or FGF21 derived amino acid sequences.
• peptide also includes dimers or multimers (oligomers) of peptides.
• dimers or multimers oligomers of the exemplified peptide sequences as well as subsequences, variants and modified forms of the exemplified peptide sequences (e.g. , sequences listed in Tables 1-10 and the Sequence Listing).
• the invention further provides nucleic acid molecules encoding peptide sequences of the invention, including subsequences, sequence variants and modified forms of the sequences listed in Tables 1-10 and the Sequence Listing, and vectors that include nucleic acid that encodes the peptide.
• nucleic acids include those that encode the exemplified peptide sequences disclosed herein, as well as those encoding functional subsequences, sequence variants and modified forms of the exemplified peptide sequences, so long as the foregoing retain at least detectable or measureable activity or function.
• a subsequence, a variant or modified form of an exemplified peptide sequence disclosed herein e.g. , a sequence listed in Tables 1 -10 and the Sequence Listing
• that retains some ability to lower or reduce glucose provide normal glucose homeostasis, or reduce the histopathological conditions associated with chronic or acute hyperglycemia in vivo, etc.
• Nucleic acid which can also be referred to herein as a gene, polynucleotide, nucleotide sequence, primer, oligonucleotide or probe refers to natural or modified purine- and pyrimidine- containing polymers of any length, either polyribonucleotides or polydeoxyribonucleotides or mixed polyribo-polydeoxyribo nucleotides and a-anomeric forms thereof.
• the two or more purine- and pyrimidine-containing polymers are typically linked by a phosphoester bond or analog thereof.
• the terms can be used interchangeably to refer to all forms of nucleic acid, including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
• the nucleic acids can be single strand, double, or triplex, linear or circular. Nucleic acids include genomic DNA and cDNA. RNA nucleic acid can be spliced or unspliced mRNA, rRNA, tRNA or antisense. Nucleic acids include naturally occurring, synthetic, as well as nucleotide analogues and derivatives.
• nucleic acid molecules include sequences degenerate with respect to nucleic acid molecules encoding the peptide sequences of the invention.
• degenerate nucleic acid sequences encoding peptide sequences including subsequences, variants and modified forms of the peptide sequences exemplified herein (e.g. , sequences listed in Tables 1-10 and the Sequence Listing), are provided.
• Nucleic acid can be produced using any of a variety of known standard cloning and chemical synthesis methods, and can be altered intentionally by site-directed mutagenesis or other recombinant techniques known to one skilled in the art. Purity of polynucleotides can be determined through sequencing, gel electrophoresis, UV spectrometry.
• Nucleic acids may be inserted into a nucleic acid construct in which expression of the nucleic acid is influenced or regulated by an "expression control element," referred to herein as an "expression cassette.”
• expression control element refers to one or more nucleic acid sequence elements that regulate or influence expression of a nucleic acid sequence to which it is operatively linked.
• An expression control element can include, as appropriate, promoters, enhancers, transcription terminators, gene silencers, a start codon (e.g. , ATG) in front of a protein-encoding gene, etc.
• An expression control element operatively linked to a nucleic acid sequence controls transcription and, as appropriate, translation of the nucleic acid sequence.
• the term "operatively linked” refers to a juxtaposition wherein the referenced components are in a relationship permitting them to function in their intended manner.
• expression control elements are juxtaposed at the 5' or the 3 ' ends of the genes but can also be intronic.
• Expression control elements include elements that activate transcription constitutively, that are inducible (i.e. , require an external signal or stimuli for activation), or derepressible (i.e. , require a signal to turn transcription off; when the signal is no longer present, transcription is activated or "derepressed”). Also included in the expression cassettes of the invention are control elements sufficient to render gene expression controllable for specific cell-types or tissues (i.e. , tissue-specific control elements). Typically, such elements are located upstream or downstream ⁇ i.e. , 5' and 3 ') of the coding sequence. Promoters are generally positioned 5' of the coding sequence. Promoters, produced by recombinant DNA or synthetic techniques, can be used to provide for transcription of the polynucleotides of the invention. A "promoter” typically means a minimal sequence element sufficient to direct transcription.
• Nucleic acids may be inserted into a plasmid for transformation into a host cell and for subsequent expression and/or genetic manipulation.
• a plasmid is a nucleic acid that can be stably propagated in a host cell; plasmids may optionally contain expression control elements in order to drive expression of the nucleic acid.
• a vector is synonymous with a plasmid. Plasmids and vectors generally contain at least an origin of replication for propagation in a cell and a promoter.
• Plasmids and vectors may also include an expression control element for expression in a host cell, and are therefore useful for expression and/or genetic manipulation of nucleic acids encoding peptide sequences, expressing peptide sequences in host cells and organisms (e.g. , a subject in need of treatment), or producing peptide sequences, for example.
• transgene means a polynucleotide that has been introduced into a cell or organism by artifice.
• a cell having a transgene the transgene has been introduced by genetic manipulation or "transformation” of the cell.
• a cell or progeny thereof into which the transgene has been introduced is referred to as a "transformed cell” or “transformant.”
• the transgene is included in progeny of the transformant or becomes a part of the organism that develops from the cell.
• Transgenes may be inserted into the chromosomal DNA or maintained as a self-replicating plasmid, YAC, minichromosome, or the like.
• Bacterial system promoters include T7 and inducible promoters such as pL of bacteriophage ⁇ , plac, ptrp, ptac (ptrp-lac hybrid promoter) and tetracycline responsive promoters.
• Insect cell system promoters include constitutive or inducible promoters (e.g. , ecdysone).
• Mammalian cell constitutive promoters include SV40, RSV, bovine papilloma virus (BPV) and other virus promoters, or inducible promoters derived from the genome of mammalian cells (e.g. , metallothionein IIA promoter; heat shock promoter) or from mammalian viruses (e.g. , the adenovirus late promoter; the inducible mouse mammary tumor virus long terminal repeat).
• a retroviral genome can be genetically modified for introducing and directing expression of a peptide sequence in appropriate host cells.
• expression systems further include vectors designed for in vivo use.
• vectors designed for in vivo use include adenoviral vectors (U.S. Patent Nos. 5,700,470 and 5,731,172), adeno-associated vectors (U.S. Patent No. 5,604,090), herpes simplex virus vectors (U.S. Patent No. 5,501,979), retroviral vectors (U.S. Patent Nos.
• Vectors include those that deliver genes to cells of the intestinal tract, including the stem cells (Croyle et ah , Gene Ther. 5:645 (1998); S.J. Henning, Adv. Drug Deliv. Rev. 17:341 (1997), U.S. Patent Nos. 5,821,235 and 6,110,456). Many of these vectors have been approved for human studies.
• Yeast vectors include constitutive and inducible promoters (see, e.g., Ausubel et ah, In:
• a constitutive yeast promoter such as ADH or LEU2 or an inducible promoter such as GAL may be used (R. Rothstein In: DNA Cloning. A Practical Approach. Vol.11, Ch. 3, ed.
• Yeast artificial chromosomes are typically used when the inserted polynucleotides are too large for more conventional vectors ⁇ e.g., greater than about 12 Kb).
• Expression vectors also can contain a selectable marker conferring resistance to a selective pressure or identifiable marker ⁇ e.g., beta-galactosidase), thereby allowing cells having the vector to be selected for, grown and expanded.
• a selectable marker can be on a second vector that is co-transfected into a host cell with a first vector containing a nucleic acid encoding a peptide sequence.
• Selection systems include but are not limited to herpes simplex virus thymidine kinase gene (Wigler et al., Cell 11 :223 (1977)), hypoxanthine-guanine phosphoribosyltransferase gene (Szybalska et al., Proc. Natl. Acad. Sci. USA 48:2026 (1962)), and adenine
• phosphoribosyltransferase (Lowy et al., Cell 22:817 (1980)) genes that can be employed in tk-, hgprt- or aprt- cells, respectively. Additionally, antimetabolite resistance can be used as the basis of selection for dhfr, which confers resistance to methotrexate (O'Hare et ah, Proc. Natl. Acad. Sci.
• transformed cell(s) in vitro, ex vivo and in vivo and host cells that produce a variant or fusion of FGF19 and/or FGF21 as set forth herein, where expression of the variant or fusion of FGF19 and/or FGF21 is conferred by a nucleic acid encoding the variant or fusion of FGF19 and/or FGF21.
• Transformed and host cells that express invention peptide sequences typically include a nucleic acid that encodes the invention peptide sequence.
• a transformed or host cell is a prokaryotic cell.
• a transformed or host cell is a eukaryotic cell.
• the eukaryotic cell is a yeast or mammalian (e.g., human, primate, etc) cell.
• a "transformed” or “host” cell is a cell into which a nucleic acid is introduced that can be propagated and/or transcribed for expression of an encoded peptide sequence.
• the term also includes any progeny or subclones of the host cell.
• Transformed and host cells include but are not limited to microorganisms such as bacteria and yeast; and plant, insect and mammalian cells.
• bacteria transformed with recombinant bacteriophage nucleic acid, plasmid nucleic acid or cosmid nucleic acid expression vectors yeast transformed with recombinant yeast expression vectors
• plant cell systems infected with recombinant virus expression vectors e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV
• plant cell systems infected with recombinant virus expression vectors e.g., cauliflower mosaic virus, CaMV; tobacco mosaic virus, TMV
• recombinant plasmid expression vectors e.g. , Ti plasmid
• insect cell systems infected with recombinant virus expression vectors e.g.
• virus expression vectors e.g., retroviruses, adenovirus, vaccinia virus
• transformed animal cell systems engineered for transient or stable propagation or expression.
• a transformed cell can be in a subject.
• a cell in a subject can be transformed with a nucleic acid that encodes an invention peptide sequence as set forth herein in vivo.
• a cell can be transformed in vitro with a transgene or
• a primary cell isolate or an established cell line can be transformed with a transgene or polynucleotide that encodes a variant of FGF19 and/or FGF21 or a fusion/chimeric sequence (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21, and then optionally transplanted into a tissue of a subject.
• a transgene or polynucleotide that encodes a variant of FGF19 and/or FGF21 or a fusion/chimeric sequence (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21, and then optionally transplanted into a tissue of a subject.
• Non-limiting target cells for expression of peptide sequences include pancreas cells (islet cells), muscle cells, mucosal cells and endocrine cells.
• pancreas cells islet cells
• muscle cells can provide inducible production (secretion) of a variant of FGF19 and/or FGF21, or a fusion/chimeric sequence (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21.
• Additional cells to transform include stem cells or other multipotent or pluripotent cells, for example, progenitor cells that differentiate into the various pancreas cells (islet cells), muscle cells, mucosal cells and endocrine cells. Targeting stem cells provides longer term expression of peptide sequences of the invention.
• the term "cultured,” when used in reference to a cell, means that the cell is grown in vitro.
• a particular example of such a cell is a cell isolated from a subject, and grown or adapted for growth in tissue culture.
• Another example is a cell genetically manipulated in vitro, and transplanted back into the same or a different subject.
• isolated when used in reference to a cell, means a cell that is separated from its naturally occurring in vivo environment. "Cultured” and “isolated” cells may be manipulated by the hand of man, such as genetically transformed. These terms include any progeny of the cells, including progeny cells that may not be identical to the parental cell due to mutations that occur during cell division. The terms do not include an entire human being.
• Nucleic acids encoding invention peptide sequences can be introduced for stable expression into cells of a whole organism. Such organisms including non-human transgenic animals are useful for studying the effect of peptide expression in a whole animal and therapeutic benefit. For example, as disclosed herein, production of a variant of FGF19 and/or FGF21 or a
• fusion/chimeric sequence (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21 as set forth herein, in mice modulated bile acid homeostasis.
• mice strains that develop or are susceptible to developing a particular disease are also useful for introducing therapeutic proteins as described herein in order to study the effect of therapeutic protein expression in the disease susceptible mouse.
• Transgenic and genetic animal models that are susceptible to particular disease or physiological conditions such as streptozotocin (STZ)-induced diabetic (STZ) mice, are appropriate targets for expressing variants of FGF19 and/or FGF21, fusions/chimeric sequences (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21 , as set forth herein.
• non-human transgenic animals that produce a variant of FGF19 and/or FGF21 , or a fusion/chimeric sequence (or variant) thereof, such as a chimeric peptide sequence including all or a portion of FGF19, or including all or a portion of FGF21 , the production of which is not naturally occurring in the animal which is conferred by a trans gene present in somatic or germ cells of the animal.
• transgenic animal refers to an animal whose somatic or germ line cells bear genetic information received, directly or indirectly, by deliberate genetic manipulation at the subcellular level, such as by microinjection or infection with recombinant virus.
• transgenic further includes cells or tissues (i.e. , "transgenic cell,” “transgenic tissue”) obtained from a transgenic animal genetically manipulated as described herein.
• a transgenic animal does not encompass animals produced by classical crossbreeding or in vitro fertilization, but rather denotes animals in which one or more cells receive a nucleic acid molecule.
• Invention transgenic animals can be either heterozygous or homozygous with respect to the transgene. Methods for producing transgenic animals, including mice, sheep, pigs and frogs, are well known in the art (see, e.g. , U.S. Patent Nos. 5,721 ,367, 5,695,977, 5,650,298, and 5,614,396) and, as such, are additionally included.
• Peptide sequences, nucleic acids encoding peptide sequences, vectors and transformed host cells expressing peptide sequences include isolated and purified forms.
• isolated when used as a modifier of an invention composition, means that the composition is separated, substantially completely or at least in part, from one or more components in an environment.
• compositions that exist in nature, when isolated, are substantially free of one or more materials with which they normally associate with in nature, for example, one or more protein, nucleic acid, lipid, carbohydrate or cell membrane.
• isolated does not exclude alternative physical forms of the composition, such as variants, modifications or derivatized forms, fusions and chimeras, multimers/oligomers, etc., or forms expressed in host cells.
• isolated also does not exclude forms (e.g. , pharmaceutical compositions, combination compositions, etc.) in which there are combinations therein, any one of which is produced by the hand of man.
• An "isolated" composition can also be “purified” when free of some, a substantial number of, or most or all of one or more other materials, such as a contaminant or an undesired substance or material.
• Peptide sequences of the invention are generally not known or believed to exist in nature. However, for a composition that does exist in nature, an isolated composition will generally be free of some, a substantial number of, or most or all other materials with which it typically associates with in nature. Thus, an isolated peptide sequence that also occurs in nature does not include polypeptides or polynucleotides present among millions of other sequences, such as proteins of a protein library or nucleic acids in a genomic or cDNA library, for example.
• purified composition includes combinations with one or more other inactive or active molecules.
• a peptide sequence of the invention combined with another drug or agent, such as a glucose lowering drug or therapeutic agent, for example.
• the term "recombinant,” when used as a modifier of peptide sequences, nucleic acids encoding peptide sequences, etc., means that the compositions have been manipulated (i.e. , engineered) in a fashion that generally does not occur in nature (e.g. , in vitro).
• a particular example of a recombinant peptide would be where a peptide sequence of the invention is expressed by a cell transfected with a nucleic acid encoding the peptide sequence.
• a particular example of a recombinant nucleic acid would be where a nucleic acid (e.g.
• genomic or cDNA encoding a peptide sequence cloned into a plasmid, with or without 5', 3 ' or intron regions that the gene is normally contiguous within the genome of the organism.
• a recombinant peptide or nucleic acid is a hybrid or fusion sequence, such as a chimeric peptide sequence comprising a portion of FGF19 and a portion of FGF21.
• compositions and mixtures of invention peptide sequences including subsequences, variants and modified forms of the exemplified peptide sequences (including the FGF19 and FGF21 variants and subsequences listed in Tables 1-10 and the Sequence Listing, and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and the Sequence Listing).
• a mixture includes one or more peptide sequences and a pharmaceutically acceptable carrier or excipient.
• a mixture in another embodiment, includes one or more peptide sequences and an adjunct drug or therapeutic agent, such as a bile acid homeostasis modulating or anti-diabetic, or glucose lowering, drug or therapeutic agent.
• an adjunct drug or therapeutic agent such as a bile acid homeostasis modulating or anti-diabetic, or glucose lowering, drug or therapeutic agent.
• Combinations such as one or more peptide sequences in a pharmaceutically acceptable carrier or excipient, with one or more of a bile acid homeostasis modulating or a treatment for a bile-acid related or associated disorder, or anti-diabetic, or glucose lowering drug or therapeutic agent are also provided.
• Such combinations of peptide sequence of the invention with another drug or agent such as a bile acid homeostasis modulating or acid related or associated disorder treating, or glucose lowering drug or therapeutic agent, for example are useful in accordance with the invention methods and uses, for example, for treatment of a subject.
• Combinations also include incorporation of peptide sequences or nucleic acids of the invention into particles or a polymeric substances, such as polyesters, carbohydrates, polyamine acids, hydrogel, polyvinyl pyrrolidone, ethylene- vinylacetate, methylcellulose, carboxymethylcellulose, protamine sulfate, or lactide/glycolide copolymers, polylactide/glycolide copolymers, or ethylenevinylacetate copolymers; entrapment in microcapsules prepared by coacervation techniques or by interfacial polymerization, for example, by the use of
• hydroxymethylcellulose or gelatin-microcapsules, or poly (methylmethacrolate) microcapsules respectively; incorporation in colloid drug delivery and dispersion systems such as macromolecule complexes, nano-capsules, microspheres, beads, and lipid-based systems (e.g., N- fatty acyl groups such as N-lauroyl, N-oleoyl, fatty amines such as dodecyl amine, oleoyl amine, etc., see US Patent No. 6,638,513), including oil-in-water emulsions, micelles, mixed micelles, and liposomes, for example.
• N- fatty acyl groups such as N-lauroyl, N-oleoyl, fatty amines such as dodecyl amine, oleoyl amine, etc.
• Invention peptides including subsequences, variants and modified forms of the exemplified peptide sequences (including the FGF19 and FGF21 variants and subsequences listed in Tables 1-10 and the Sequence Listing, and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and the Sequence Listing) as set forth herein can be used to modulate glucose metabolism and facilitate transport of glucose from the blood to key metabolic organs such as muscle, liver and fat.
• Such peptide sequences can be produced in amounts sufficient or effective to restore glucose tolerance and/or to improve or provide normal glucose homeostasis.
• invention peptides including subsequences, variants and modified forms of the exemplified peptide sequences (including the FGF19 and FGF21 variants and subsequences listed in Tables 1-10 and the Sequence Listing, and the FGF19/FGF21 fusions and chimeras listed in Tables 1-10 and the Sequence Listing), into an animal, either by direct or indirect in vivo or by ex vivo methods (e.g., administering the variant or fusion peptide, a nucleic acid encoding the variant or fusion peptide, or a transformed cell or gene therapy vector expressing the variant or fusion peptide), can be used to treat various disorders, such as bile-acid related or associated disorders.
• disorders such as bile-acid related or associated disorders.
• the invention includes in vitro, ex vivo and in vivo (e.g., on or in a subject) methods and uses. Such methods and uses can be practiced with any of the peptide sequences of the invention set forth herein.
• a method includes administering a peptide sequence, such as an FGF19 or FGF21 variant, fusion or chimera disclosed herein (see, e.g., Tables 1-10), or a subsequence, a variant or modified form of an FGF19 or FGF21 variant, fusion or chimera disclosed herein (see, e.g., Tables 1-10 and the Sequence Listing), to a subject in an amount effective for treating the disorder.
• a peptide sequence such as an FGF19 or FGF21 variant, fusion or chimera disclosed herein (see, e.g., Tables 1-10), or a subsequence, a variant or modified form of an FGF19 or FGF21 variant, fusion or chimera disclosed herein (see, e.g., Tables 1-10 and the Sequence Listing)
• Exemplary disorders treatable, preventable, and the like with invention peptides, and methods and uses include bile-acid related or associated disorders.
• diseases and disorders include: metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (i)
• invention peptide sequences can be administered to subjects in need of modulation of bile acid homeostasis or having a bile-acid related or associated disorder.
• Peptide sequences of the invention may also be useful in other hyperglycemic-related disorders, including kidney damage (e.g., tubule damage or nephropathy), liver degeneration, eye damage (e.g., diabetic retinopathy or cataracts), and diabetic foot disorders; Dyslipidemias and their sequelae such as, for example, atherosclerosis, coronary artery disease, cerebrovascular disorders and the like.
• Other conditions which may be associated with metabolic syndrome such as obesity and elevated body mass (including the co-morbid conditions thereof such as, but not limited to, nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and polycystic ovarian syndrome (PCOS)), and also include thromboses, hypercoagulable and prothrombotic states
• hypertension including portal hypertension (defined as a hepatic venous pressure gradient (HVPG) greater than 5 mm Hg), cardiovascular disease, stroke and heart failure;
• HVPG hepatic venous pressure gradient
• disorders or conditions in which inflammatory reactions are involved including atherosclerosis, chronic inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), asthma, lupus erythematosus, arthritis, or other inflammatory rheumatic disorders; Disorders of cell cycle or cell differentiation processes such as adipose cell tumors, lipomatous carcinomas including, for example, liposarcomas, solid tumors, and neoplasms; Neurodegenerative diseases and/or demyelinating disorders of the central and peripheral nervous systems and/or neurological diseases involving neuroinflammatory processes and/or other peripheral neuropathies, including Alzheimer's disease, multiple sclerosis, Parkinson's disease, progressive multifocal leukoencephalopathy and Guillian- Barre syndrome; Skin and dermatological disorders and/or disorders of wound healing processes, including erythemato-squamous dermatoses; and other disorders such as syndrome X, osteoarthritis, and acute respiratory distress syndrome.
• atherosclerosis chronic inflammatory bowel diseases (
• the term "bile-acid related or associated disorder,” when used in reference to a condition of a subject means a transient or chronic abnormal level of a bile acid (one or more bile acids) present in the subject.
• the condition can be caused by inhibition, reduction or a delay in bile acid synthesis, metabolism or absorption such that the subject exhibits a bile acid level not typically found in normal subjects.
• the invention includes methods of preventing (e.g. , in subjects predisposed to having a particular disorder(s)), delaying, slowing or inhibiting progression of, the onset of, or treating (e.g. , ameliorating) a bile-acid related or associated disorder relative to an appropriate matched subject of comparable age, gender, race, etc.).
• a method of the invention for, for example, modulating bile acid homeostasis or treating a bile-acid related or associated disorder includes contacting or administering a peptide of the invention as set forth herein (e.g.
• FGF19 and/or FGF21 as set forth in Tables 1-10 or the Sequence Listing, for example) in an amount effective to modulate bile acid homeostasis or treat a bile-acid related or associated disorder.
• the invention includes methods of preventing (e.g. , in subjects predisposed to having a particular disorder(s)), slowing or inhibiting the progression of, delaying the onset of, or treating undesirable levels or abnormally low levels of bile acids, all of which, alone or in combination, can lead to, for example, to at a bile-acid related or associated disorder.
• Such disorders can be due to, for example, genetic predisposition or diet, for example.
• subject refers to an animal.
• the animal is a mammal that would benefit from treatment with a peptide sequence of the invention.
• Particular examples include primates (e.g. , humans), dogs, cats, horses, cows, pigs, and sheep.
• Subjects include those having a disorder, e.g. , a bile acid related or associated disorder, such as metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis
• a disorder e.g. , a bile acid related or associated disorder, such as metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis
• bile acid malabsorption and other disorders involving the distal small intestine including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea (e.g., BAD) and GI symptoms, and GI, liver, and/or biliary cancers (e.g., colon cancer and hepatocellular cancer); and/or bile acid synthesis abnormalities, such as those contributing to NASH, cirrhosis and portal hypertension; or subjects that do not have a disorder but may be at risk of developing the disorder
• Subjects at risk of developing a bile acid associated or related disorder include, for example, those whose diet may contribute to development of acute or metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea (e.g., BAD)
• treatment methods include contacting or administering a peptide of the invention as set forth herein (e.g., a variant or fusion of FGF19 and or FGF21 as set forth in
• a treatment that results in a desired outcome or result includes decreasing, reducing or preventing severity or frequency of one or more symptoms of the condition in the subject, e.g., an improvement in the subject's condition or a "beneficial effect” or "therapeutic effect.” Therefore, treatment can decrease or reduce or prevent the severity or frequency of one or more symptoms of the disorder, stabilize or inhibit progression or worsening of the disorder, and in some instances, reverse the disorder, transiently (e.g., for 1-6, 6-12, or 12-24 hours), for medium term (e.g., 1-6, 6-12, 12-24 or 24-48 days) or long term (e.g., for 1-6, 6-12, 12-24, 24-48 weeks, or greater than 24-48 weeks).
• transiently e.g., for 1-6, 6-12, or 12-24 hours
• medium term e.g., 1-6, 6-12, 12-24 or 24-48 days
• long term e.g., for 1-6, 6-12, 12-24, 24-48 weeks, or greater than 24-48 weeks.
• a bile acid related or associated disorder such as metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea (e.g., BAD) and GI symptoms, and GI, liver, and/or
• an "effective amount” or a "sufficient amount” for use and/or for treating a subject refer to an amount that provides, in single or multiple doses, alone, or in combination with one or more other compositions (therapeutic agents such as a drug or treatment for hyperglycemia), treatments, protocols, or therapeutic regimens agents, a detectable response of any duration of time (transient, medium or long term), a desired outcome in or an objective or subjective benefit to a subject of any measurable or detectable degree or for any duration of time (e.g., for hours, days, months, years, or cured).
• Such amounts typically are effective to ameliorate a disorder, or one, multiple or all adverse symptoms, consequences or complications of the disorder, to a measurable extent, although reducing or inhibiting a progression or worsening of the disorder, is considered a satisfactory outcome.
• the term “ameliorate” means an improvement in the subject's disorder, a reduction in the severity of the disorder, or an inhibition of progression or worsening of the disorder
• a bile acid related or associated disorder e.g., metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC,
• PSC neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea (e.g., BAD) and GI symptoms, and GI, liver, and/or biliary cancers (e.g., colon cancer and hepatocellular cancer); and/or bile acid synthesis abnormalities, such as those contributing to NASH, cirrhosis and portal hypertension; for example, an improvement can be a lowering or a reduction in one or more symptoms or effects of the disorder.
• a therapeutic benefit or improvement therefore need not be complete ablation of any one, most or all symptoms, complications, consequences or underlying causes associated with the disorder or disease.
• a satisfactory endpoint is achieved when there is a transient, medium or long term, incremental improvement in a subject's condition, or a partial reduction in the occurrence, frequency, severity, progression, or duration, or inhibition or reversal, of one or more associated adverse symptoms or complications or consequences or underlying causes, worsening or progression (e.g. , stabilizing one or more symptoms or complications of the condition, disorder or disease), of the disorder or disease, over a duration of time (hours, days, weeks, months, etc.).
• the amount of peptide sufficient to ameliorate a disorder will depend on the type, severity and extent, or duration of the disorder, the therapeutic effect or outcome desired, and can be readily ascertained by the skilled artisan. Appropriate amounts will also depend upon the individual subject (e.g. , the bioavailability within the subject, gender, age, etc.).
• a transient, or partial, restoration of normal bile acid homeostasis in a subject can reduce the dosage amount or frequency of a drug used to treat metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis
• bile cut compression from tumor, bile duct blockade by gall stones e.g., bile cut compression from tumor, bile duct blockade by gall stones
• diseases of extrahepatic cholestasis e.g., bile cut compression from tumor, bile duct blockade by gall stones
• diseases of extrahepatic cholestasis e.g., bile cut compression from tumor, bile duct blockade by gall stones
• bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea e.g.,
• BAD BAD
• GI symptoms and GI, liver, and/or biliary cancers (e.g., colon cancer and hepatocellular cancer); and/or bile acid synthesis abnormalities, such as those contributing to NASH, cirrhosis and portal hypertension; even though complete freedom from treatment has not resulted.
• An effective amount can be ascertained, for example, by measuring one or more relevant physiological effects. .
• Methods and uses of the invention for treating a subject are applicable for prophylaxis to prevent or reduce likelihood of a disorder in a subject, such as a bile acid related or associated disorder.
• methods and uses can be practiced during or following treatment of a subject.
• a method or use of the invention can, for example, a peptide sequence of the invention can be administered to the subject.
• a composition such as a peptide sequence of the invention can be combined with another drug or agent, such as a bile acid stabilizing drug or therapeutic agent, for example.
• Supplementary therapies include other glucose lowering treatments, such as insulin, an insulin sensitivity enhancer and other drug treatments, a change in diet (low sugar, fats, etc.), weight loss surgery- (reducing stomach volume by gastric bypass,
• a method or use of the invention for treating a hyperglycemic or insulin resistance disorder can be used in combination with drugs or other pharmaceutical compositions that lower glucose or increase insulin sensitivity in a subject.
• the present disclosure contemplates combination therapy with numerous agents (and classes thereof), including 1) insulin e.g., bolus and basal analogs), insulin mimetics and agents that entail stimulation of insulin secretion, including sulfonylureas (e.g. , chlorpropamide, tolazamide, acetohexamide, tolbutamide, glyburide, glimepiride, glipizide) and meglitinides (e.g. , repaglinide
• PRANDIN PRANDIN and nateglinide (STARLIX)
• biguanides e.g. , metformin (GLUCOPHAGE)
• alpha-glucosidase inhibitors e.g. , acarbose and miglitol
• thiazolidinediones e.g. , rosiglitazone (AVANDIA), troglitazone (REZULIN), pioglitazone (ACTOS), glipizide, balaglitazone, rivoglitazone,
• glucagon-like-peptides including DPP-IV inhibitors (e.g. , vildagliptin (GALVUS) and sitagliptin
• GLP-1 Glucagon-Like Peptide- 1
• GLP-1 agonists and analogs e.g. , exenatide
• BYETTA and ITCA 650 an osmotic pump inserted subcutaneously that delivers an exenatide analog over a 12-month period; Intarcia, Boston, MA
• DPP-IV-resistant analogues cretin mimetics
• PPAR gamma agonists dual-acting PPAR agonists, pan-acting PPAR agonists, PTP1B inhibitors, SGLT inhibitors, insulin secretagogues, RXR agonists, glycogen synthase kinase-3 inhibitors, immune modulators, beta-3 adrenergic receptor agonists, 1 lbeta-HSDl inhibitors, and amylin analogues.
• DPP-4 dipeptidyl peptidase-4
• bromocriptine formulations e.g. and bile acid sequestrants (e.g., colesevelam)
• SGLT-2 inhibitors e.g., glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, glycerol, g., g., bile acid sequestrants (e.g., colesevelam), and SGLT-2 inhibitors.
• Appetite suppression drugs are also well known and can be used in combination with the compositions and methods provided herein.
• Supplementary therapies can be administered prior to, contemporaneously with or following invention methods and uses.
• Peptide sequences of the invention including subsequences, sequence variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing), may be formulated in a unit dose or unit dosage form.
• sequences listed in Tables 1-10 and the Sequence Listing may be formulated in a unit dose or unit dosage form.
• a peptide sequence is in an amount effective to treat a subject in need of treatment, e.g., due to abnormal or aberrant bile acid homeostasis, such as metabolic syndrome; a lipid- or glucose- related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic
• Exemplary unit doses range from about 25-250, 250-500, 500-1000, 1000-2500 or 2500-5000, 5000-25,000, 25,000-50,000 ng; from about 25-250, 250-500, 500-1000, 1000-2500 or 2500-5000, 5000-25,000, 25,000-50,000 ⁇ g; and from about 25-250, 250-500, 500-1000, 1000-2500 or 2500-5000, 5000- 25,000, 25,000-50,000 mg.
• Peptide sequences of the invention including subsequences, sequence variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing) can be administered to provide the intended effect as a single dose or multiple dosages, for example, in an effective or sufficient amount.
• Exemplary doses range from about 25- 250, 250-500, 500-1000, 1000-2500 or 2500-5000, 5000-25,000, 25,000-50,000 pg/kg; from about 50-500, 500-5000, 5000-25,000 or 25,000-50,000 ng/kg; and from about 25-250, 250-500, 500-1000, 1000-2500 or 2500-5000, 5000-25,000, 25,000-50,000 ⁇ .
• Single or multiple doses can be administered, for example, multiple times per day, on consecutive days, alternating days, weekly or intermittently (e.g. , twice per week, once every 1 , 2, 3, 4, 5, 6, 7 or 8 weeks, or once every 2, 3, 4, 5 or 6 months).
• Peptide sequences of the invention including subsequences, variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing) can be administered and methods may be practiced via systemic, regional or local administration, by any route.
• a peptide sequence can be administered parenterally (e.g. , subcutaneously, intravenously, intramuscularly, or intraperitoneally), orally (e.g. , ingestion, buccal, or sublingual), inhalation, intradermally, intracavity, intracranially, transdermally (topical), transmucosally or rectally.
• Peptide sequences of the invention including subsequences, variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing) and methods of the invention including pharmaceutical compositions can be administered via a
• a particular non- limiting example of parenteral (e.g. , subcutaneous) administration entails the use of Intarcia's subcutaneous delivery system (Intarcia Therapeutics, Inc.; Hayward, CA).
• the system comprises a miniature osmotic pump that delivers a consistent amount of a therapeutic agent over a desired period of time.
• the system can be used with formulations that maintain the stability of proteinaceous therapeutic agents at human body temperature for extended periods of time.
• the invention further provides "pharmaceutical compositions," which include a peptide sequence (or sequences) of the invention, including subsequences, variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing), and one or more pharmaceutically acceptable or physiologically acceptable diluent, carrier or excipient.
• a peptide sequence or sequences are present in a therapeutically acceptable amount.
• the pharmaceutical compositions may be used in accordance with the invention methods and uses.
• the pharmaceutical compositions can be administered ex vivo or in vivo to a subject in order to practice treatment methods and uses of the invention.
• compositions of the invention can be formulated to be compatible with the intended method or route of administration; exemplary routes of administration are set forth herein.
• the pharmaceutical compositions may further comprise other therapeutically active agents or compounds disclosed herein (e.g. , bile acid stabilizing agents or drugs) or known to the skilled artisan which can be used in the treatment or prevention of various bile acid diseases and disorders as set forth herein.
• compositions typically comprise a therapeutically effective amount of at least one of the peptide sequences of the invention, including subsequences, variants and modified forms of the exemplified peptide sequences (sequences listed in Tables 1-10 and the Sequence Listing) and one or more pharmaceutically and physiologically acceptable formulation agents.
• Suitable pharmaceutically acceptable or physiologically acceptable diluents, carriers or excipients include, but are not limited to, antioxidants (e.g., ascorbic acid and sodium bisulfate), preservatives (e.g., benzyl alcohol, methyl parabens, ethyl or n-propyl, p-hydroxybenzoate), emulsifying agents, suspending agents, dispersing agents, solvents, fillers, bulking agents, buffers, vehicles, diluents, and/or adjuvants.
• a suitable vehicle may be physiological saline solution or citrate buffered saline, possibly supplemented with other materials common in pharmaceutical compositions for parenteral administration.
• Neutral buffered saline or saline mixed with serum albumin are further exemplary vehicles.
• Typical buffers include, but are not limited to pharmaceutically acceptable weak acids, weak bases, or mixtures thereof.
• Buffer components also include water soluble materials such as phosphoric acid, tartaric acids, lactic acid, succinic acid, citric acid, acetic acid, ascorbic acid, aspartic acid, glutamic acid, and salts thereof.
• a primary solvent in a vehicle may be either aqueous or non-aqueous in nature.
• the vehicle may contain other pharmaceutically acceptable excipients for modifying or maintaining the pH, osmolarity, viscosity, sterility or stability of the pharmaceutical composition.
• the pharmaceutically acceptable vehicle is an aqueous buffer.
• a vehicle comprises, for example, sodium chloride and/or sodium citrate.
• compositions of the invention may contain still other pharmaceutically- acceptable formulation agents for modifying or maintaining the rate of release of an invention peptide.
• formulation agents include those substances known to artisans skilled in preparing sustained release formulations.
• physiologically acceptable formulation agents see, for example, Remington's Pharmaceutical Sciences. 18th Ed. (1990, Mack Publishing Co., Easton, Pa. 18042) pages 1435-1712, The Merck Index, 12th Ed. (1996, Merck Publishing Group, Whitehouse, NJ); and Pharmaceutical Principles of Solid Dosage Forms (1993, Technonic Publishing Co., Inc., Lancaster, Pa.). Additional pharmaceutical compositions appropriate for administration are known in the art and are applicable in the methods and compositions of the invention.
• a pharmaceutical composition may be stored in a sterile vial as a solution, suspension, gel, emulsion, solid, or dehydrated or lyophilized powder. Such compositions may be stored either in a ready to use form, a lyophilized form requiring reconstitution prior to use, a liquid form requiring dilution prior to use, or other acceptable form.
• a pharmaceutical composition is provided in a single-use container (e.g., a single-use vial, ampoule, syringe, or autoinjector (similar to, e.g., an EpiPen®)), whereas a multi-use container (e.g., a multi-use vial) is provided in other embodiments.
• Any drug delivery apparatus may be used to deliver invention peptides, including implants (e.g., implantable pumps) and catheter systems, both of which are known to the skilled artisan.
• Depot injections which are generally administered subcutaneously or intramuscularly, may also be utilized to release invention peptides over a defined period of time. Depot injections are usually either solid- or oil-based and generally comprise at least one of the formulation components set forth herein. The skilled artisan is familiar with possible formulations and uses of depot injections.
• a pharmaceutical composition can be formulated to be compatible with its intended route of administration.
• pharmaceutical compositions include carriers, diluents, or excipients suitable for administration by routes including parenteral (e.g., subcutaneous (s.c), intravenous, intramuscular, or intraperitoneal), intradermal, oral (e.g., ingestion), inhalation, intracavity, intracranial, and transdermal (topical).
• parenteral e.g., subcutaneous (s.c), intravenous, intramuscular, or intraperitoneal
• intradermal e.g., oral (e.g., ingestion), inhalation, intracavity, intracranial, and transdermal (topical).
• compositions may be in the form of a sterile injectable aqueous or oleagenous suspension.
• This suspension may be formulated using suitable dispersing or wetting agents and suspending agents disclosed herein or known to the skilled artisan.
• the sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally- acceptable diluent or solvent, for example, as a solution in 1 ,3-butane diol.
• Acceptable diluents, solvents and dispersion media include water, Ringer's solution, isotonic sodium chloride solution, Cremophor ELTM (BASF, Parsippany, NJ) or phosphate buffered saline
• PBS polyol
• polyol e.g., glycerol, propylene glycol, and liquid polyethylene glycol
• suitable mixtures thereof sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil may be employed including synthetic mono- or diglycerides.
• fatty acids such as oleic acid find use in the preparation of injectables. Prolonged absorption of particular injectable formulations can be achieved by including an agent that delays absorption (e.g., aluminum monostearate or gelatin).
• compositions may be in a form suitable for oral use, for example, as tablets, capsules, troches, lozenges, aqueous or oily suspensions, dispersible powders or granules, emulsions, hard or soft capsules, or syrups, solutions, microbeads or elixirs.
• Pharmaceutical compositions intended for oral use may be prepared according to any method known to the art for the manufacture of pharmaceutical compositions. Such compositions may contain one or more agents such as sweetening agents, flavoring agents, coloring agents and preserving agents in order to provide pharmaceutically elegant and palatable preparations. Tablets containing an invention peptide may be in admixture with non-toxic pharmaceutically acceptable excipients suitable for the manufacture of tablets.
• excipients include, for example, diluents, such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate; granulating and disintegrating agents, for example, corn starch, or alginic acid; binding agents, for example starch, gelatin or acacia, and lubricating agents, for example magnesium stearate, stearic acid or talc.
• diluents such as calcium carbonate, sodium carbonate, lactose, calcium phosphate or sodium phosphate
• granulating and disintegrating agents for example, corn starch, or alginic acid
• binding agents for example starch, gelatin or acacia
• lubricating agents for example magnesium stearate, stearic acid or talc.
• Tablets, capsules and the like suitable for oral administration may be uncoated or they may be coated by known techniques to delay disintegration and absorption in the gastrointestinal tract and thereby provide a sustained action over a longer period.
• a time delay material such as glyceryl monostearate or glyceryl distearate may be employed. They may also be coated by techniques known in the art to form osmotic therapeutic tablets for controlled release.
• Additional agents include biodegradable or biocompatible particles or a polymeric substance such as polyesters, polyamine acids, hydrogel, polyvinyl pyrrolidone, polyanhydrides, polyglycolic acid, ethylene- vinylacetate, methylcellulose, carboxymethylcellulose, protamine sulfate, or lactide/glycolide copolymers, polylactide/glycolide copolymers, or ethyl enevinylacetate copolymers in order to control delivery of an administered composition.
• a polymeric substance such as polyesters, polyamine acids, hydrogel, polyvinyl pyrrolidone, polyanhydrides, polyglycolic acid, ethylene- vinylacetate, methylcellulose, carboxymethylcellulose, protamine sulfate, or lactide/glycolide copolymers, polylactide/glycolide copolymers, or ethyl enevinylacetate copolymers in order to control delivery of an administered composition.
• the oral agent can be entrapped in microcapsules prepared by coacervation techniques or by interfacial polymerization, by the use of hydroxymethylcellulose or gelatin-microcapsules or poly (methylmethacrolate) microcapsules, respectively, or in a colloid drug delivery system.
• Colloidal dispersion systems include
• macromolecule complexes including oil-in- water emulsions, micelles, mixed micelles, and liposomes.
• Methods for preparation of such formulations are known to those skilled in the art and are commercially available.
• Formulations for oral use may also be presented as hard gelatin capsules wherein the active ingredient is mixed with an inert solid diluent, for example, calcium carbonate, calcium phosphate, kaolin or microcrystalline cellulose, or as soft gelatin capsules wherein the active ingredient is mixed with water or an oil medium, for example peanut oil, liquid paraffin, or olive oil.
• an inert solid diluent for example, calcium carbonate, calcium phosphate, kaolin or microcrystalline cellulose
• water or an oil medium for example peanut oil, liquid paraffin, or olive oil.
• Aqueous suspensions contain the active materials in admixture with excipients suitable for the manufacture thereof. Such excipients are suspending agents, for example sodium
• dispersing or wetting agents may be a naturally-occurring phosphatide, for example lecithin, or condensation products of an alkylene oxide with fatty acids, for example polyoxy-ethylene stearate, or condensation products of ethylene oxide with long chain aliphatic alcohols, for example heptadecaethyleneoxycetanol, or condensation products of ethylene oxide with partial esters derived from fatty acids and a hexitol such as polyoxyethylene sorbitol monooleate, or condensation products of ethylene oxide with partial esters derived from fatty acids and hexitol anhydrides, for example polyethylene sorbitan monooleate.
• the aqueous suspensions may also contain one or more preservatives.
• Oily suspensions may be formulated by suspending the active ingredient in a vegetable oil, for example arachis oil, olive oil, sesame oil or coconut oil, or in a mineral oil such as liquid paraffin.
• the oily suspensions may contain a thickening agent, for example beeswax, hard paraffin or cetyl alcohol. Sweetening agents such as those set forth above, and flavoring agents may be added to provide a palatable oral preparation.
• Dispersible powders and granules suitable for preparation of an aqueous suspension by addition of water provide the active ingredient in admixture with a dispersing or wetting agent, suspending agent and one
• compositions of the invention may also be in the form of oil-in-water emulsions.
• the oily phase may be a vegetable oil, for example olive oil or arachis oil, or a mineral oil, for example, liquid paraffin, or mixtures of these.
• Suitable emulsifying agents may be naturally- occurring gums, for example, gum acacia or gum tragacanth; naturally-occurring phosphatides, for example, soy bean, lecithin, and esters or partial esters derived from fatty acids; hexitol anhydrides, for example, sorbitan monooleate; and condensation products of partial esters with ethylene oxide, for example, polyoxyethylene sorbitan monooleate.
• compositions can also include carriers to protect the composition against rapid degradation or elimination from the body, such as a controlled release formulation, including implants, liposomes, hydrogels, prodrugs and microencapsulated delivery systems.
• a time delay material such as glyceryl monostearate or glyceryl stearate alone, or in combination with a wax, may be employed.
• Prolonged absorption of injectable pharmaceutical compositions can be achieved by including an agent that delays absorption, for example, aluminum monostearate or gelatin.
• Prevention of the action of microorganisms can be achieved by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, ascorbic acid, thimerosal, and the like.
• the invention also includes invention peptides in the form of suppositories for rectal administration.
• the suppositories can be prepared by mixing an invention peptide with a suitable non-irritating excipient which is solid at ordinary temperatures but liquid at the rectal temperature and will therefore melt in the rectum to release the drug.
• suitable non-irritating excipient include, but are not limited to, cocoa butter and polyethylene glycols.
• a method includes: providing a candidate peptide sequence; administering the candidate peptide sequence to a test animal; measuring bile acid levels of the animal after administration of the candidate peptide sequence, to determine if the candidate peptide sequence modulates bile acid homeostasis; and analyzing the candidate peptide sequence for induction of HCC in the animal, or expression of a marker correlating with HCC activity.
• a candidate peptide that modulates bile acid homeostasis but does not have substantial HCC activity thereby identifies a peptide sequence having that modulates bile acid homeostasis without substantial HCC activity.
• bile acids and precursors such as 7 alpha-hydroxy-4- cholesten-3-one
• a sample e.g., serum
• Another non- limiting examples is a two reaction method (Randox Laboratories, Ltd.) using serum or heparinized plasma.
• bile acids are oxidized by 3-a-hydroxysteroid dehydrogenase with the subsequent reduction of Thio-NAD to Thio-NADH.
• oxidized bile acids are reduced by the same enzyme with the subsequent oxidation of NADH to NAD.
• the rate of formation of Thio-NADH is determined by measuring the specific absorbance change at 405 nm.
• HCC liver cancer
• risk factors for HCC include type 2 diabetes (probably exacerbated by obesity).
• the risk of HCC in type 2 diabetics is greater (from -2.5 to ⁇ 7 times the non-diabetic risk) depending on the duration of diabetes and treatment protocol.
• Indicators for HCC include detection of a tumor maker such as elevated alpha- fetoprotein (AFP) or des-gamma carboxyprothrombin (DCP) levels.
• AFP alpha- fetoprotein
• DCP des-gamma carboxyprothrombin
• a number of different scanning and imaging techniques are also helpful, including ultrasound, CT scans and MRI.
• liver cancer may be nodular, where the tumor nodules (which are round-to-oval, grey or green, well circumscribed but not encapsulated) appear as either one large mass or multiple smaller masses.
• HCC may be present as an infiltrative tumor which is diffuse and poorly circumscribed and frequently infiltrates the portal veins.
• Methods of the invention may further include assessing a hepatic tissue sample from an in vivo animal model (e.g., a db/db mouse) useful in HCC studies in order to determine whether a peptide sequence exhibits evidence of inducing HCC.
• an in vivo animal model e.g., a db/db mouse
• a pathologist can determine whether one of the four general architectural and cytological types (patterns) of HCC are present (i.e., fibrolamellar, pseudoglandular (adenoid), pleomorphic (giant cell) and clear cell).
• the invention also includes the generation and use of antibodies, and fragments thereof, that bind the peptide sequences of the invention, including subsequences, sequence variants and modified forms of the exemplified peptide sequences (including the peptides listed in Tables 1-10 and the Sequence Listing).
• antibodies As used herein, the terms “antibodies” (Abs) and “immunoglobulins” (Igs) refer to glycoproteins having the same structural characteristics. While antibodies exhibit binding specificity to an antigen, immunoglobulins include both antibodies and other antibody-like molecules which may lack antigen specificity.
• antibody includes intact monoclonal antibodies, polyclonal antibodies, multispecific antibodies (e.g., bispecific antibodies) formed from at least two intact antibodies, and antibody binding fragments including Fab and F(ab) ! 2, provided that they exhibit the desired biological activity.
• the basic antibody structural unit comprises a tetramer, and each tetramer is composed of two identical pairs of polypeptide chains, each pair having one "light” chain (about 25 kDa) and one "heavy” chain (about 50-70 kDa).
• the amino-terminal portion of each chain includes a variable region of about 100 to 110 or more amino acids primarily responsible for antigen recognition.
• each chain defines a constant region primarily responsible for effector function.
• Human light chains are classified as kappa and lambda light chains, whereas human heavy chains are classified as mu, delta, gamma, alpha, or epsilon, and define the antibody's isotype as IgM, IgD, IgA, and IgE, respectively.
• Binding fragments are produced by recombinant DNA techniques, or by enzymatic or chemical cleavage of intact antibodies. Binding fragments include Fab, Fab', F(ab')2, Fv, and single-chain antibodies.
• Each heavy chain has at one end a variable domain (VH) followed by a number of constant domains.
• Each light chain has a variable domain at one end (VL) and a constant domain at its other end; the constant domain of the light chain is aligned with the first constant domain of the heavy chain, and the light chain variable domain is aligned with the variable domain of the heavy chain.
• the variable and constant regions are joined by a "J" region of about 12 or more amino acids, with the heavy chain also including a "D" region of about 10 more amino acids.
• the antibody chains all exhibit the same general structure of relatively conserved framework regions (FR) joined by three hyper- variable regions, also called complementarity- determining regions or CDRs.
• both light and heavy chains comprise the domains FR1 , CDR1 , FR2, CDR2, FR3, CDR3 and FR4.
• An intact antibody has two binding sites and, except in bifunctional or bispecific antibodies, the two binding sites are the same.
• a bispecific or bifunctional antibody is an artificial hybrid antibody having two different heavy/light chain pairs and two different binding sites.
• Bispecific antibodies can be produced by a variety of methods including fusion of hybridomas or linking of Fab' fragments.
• the term "monoclonal antibody” refers to an antibody obtained from a population of substantially homogeneous antibodies, that is, the individual antibodies comprising the population are identical except for possible naturally occurring mutations that may be present in minor amounts. Monoclonal antibodies are highly specific, being directed against a single antigenic site. In contrast to polyclonal antibody preparations which include different antibodies directed against different determinants (epitopes), each monoclonal antibody is directed against a single determinant on the antigen.
• a “neutralizing antibody” is an antibody molecule that is able to eliminate or
• Antibody binding fragments may be produced by enzymatic or chemical cleavage of intact antibodies. Digestion of antibodies with the enzyme papain results in two identical antigen- binding fragments, also known as "Fab" fragments, and an "Fc” fragment which has no antigen- binding activity. Digestion of antibodies with the enzyme pepsin results in a F(ab')2 fragment in which the two arms of the antibody molecule remain linked and comprise two-antigen binding sites. The F(ab')2 fragment has the ability to crosslink antigen.
• Fab refers to a fragment of an antibody that comprises the constant domain of the light chain and the CHI domain of the heavy chain.
• Fv when used herein refers to the minimum fragment of an antibody that retains both antigen-recognition and antigen-binding sites. In a two-chain Fv species, this region consists of a dimer of one heavy-chain and one light-chain variable domain in non-covalent association. In a single-chain Fv species, one heavy-chain and one light-chain variable domain can be covalently linked by a flexible peptide linker such that the light and heavy chains can associate in a "dimeric" structure analogous to that in a two-chain Fv species.
• variable domain interacts to define an antigen- binding site on the surface of the VH-VL dimer. While the six CDRs, collectively, confer antigen- binding specificity to the antibody, even a single variable domain (or half of an Fv comprising only three CDRs specific for an antigen) has the ability to recognize and bind antigen.
• CDRs complementarity determining regions
• hypervariable region refers to the amino acid residues of an antibody which are responsible for antigen-binding.
• the hypervariable region generally comprises amino acid residues from a “complementarity determining region” or “CDR” and/or those residues from a “hypervariable loop”.
• epitope refers to binding sites for antibodies on protein antigens.
• Epitopic determinants usually consist of chemically active surface groupings of molecules such as amino acids or sugar side chains, as well as specific three dimensional structural and charge characteristics.
• An antibody is said to bind an antigen when the dissociation constant is ⁇ 1 ⁇ , preferably ⁇ 100 nM, and most preferably ⁇ 10 nM.
• An increased equilibrium constant means that there is less affinity between the epitope and the antibody, whereas a decreased equilibrium constant means that there is a higher affinity between the epitope and the antibody.
• an antibody with a ⁇ ⁇ of "no more than” a certain amount means that the antibody will bind to the epitope with the given ⁇ ⁇ or more strongly.
• ⁇ ⁇ describes the binding characteristics of an epitope and an antibody
• potency describes the effectiveness of the antibody itself for a function of the antibody. There is not necessarily a correlation between an equilibrium constant and potency; thus, for example, a relatively low KD does not automatically mean a high potency.
• the term "selectively binds" in reference to an antibody does not mean that the antibody only binds to a single substance, but rather that the ⁇ ⁇ of the antibody to a first substance is less than the KQ of the antibody to a second substance. An antibody that exclusively binds to an epitope only binds to that single epitope.
• antibodies that contain rodent (murine or rat) variable and/or constant regions are sometimes associated with, for example, rapid clearance from the body or the generation of an immune response by the body against the antibody.
• rodent-derived antibodies In order to avoid the utilization of rodent-derived antibodies, fully human antibodies can be generated through the introduction of human antibody function into a rodent so that the rodent produces fully human antibodies.
• human and “fully human” antibodies can be used interchangeably herein. The term “fully human” can be useful when distinguishing antibodies that are only partially human from those that are completely, or fully human. The skilled artisan is aware of various methods of generating fully human antibodies.
• Chimeric or otherwise humanized antibodies can be utilized. Chimeric antibodies have a human constant region and a murine variable region, and, as such, human anti-chimeric antibody responses may be observed in some patients. Therefore, it is advantageous to provide fully human antibodies against multimeric enzymes in order to avoid possible human anti-mouse antibody or human anti-chimeric antibody responses.
• Fully human monoclonal antibodies can be prepared, for example, by the generation of hybridoma cell lines by techniques known to the skilled artisan. Other preparation methods involve the use of sequences encoding particular antibodies for transformation of a suitable mammalian host cell, such as a CHO cell. Transformation can be by any known method for introducing
• polynucleotides into a host cell including, for example, packaging the polynucleotide in a virus (or into a viral vector) and transducing a host cell with the virus (or vector) or by transfection procedures known in the art.
• Methods for introducing heterologous polynucleotides into mammalian cells are well known in the art and include dextran-mediated transfection, calcium phosphate precipitation, polybrene-mediated transfection, protoplast fusion, electroporation, encapsulation of the
• Mammalian cell lines available as hosts for expression are well known in the art and include, but are not limited to CHO cells, HeLa cells, and human hepatocellular carcinoma cells.
• Antibodies can be used diagnostically and/or therapeutically.
• the antibodies can be used as a diagnostic by detecting the level of one or more peptides of the invention in a subject, and either comparing the detected level to standard control level or to a baseline level in a subject determined previously (e.g. , prior to any illness).
• the antibodies can be used as a therapeutic to modulate the activity of one or more peptides of the invention, thereby having an effect on a condition or disorder.
• kits including, but not limited to, peptide sequences of the invention, optionally in combination with one or more therapeutic agents, compositions and pharmaceutical compositions thereof, packaged into suitable packaging material.
• a kit optionally includes a label or packaging insert including a description of the components or instructions for use in vitro, in vivo, or ex vivo, of the components therein.
• Exemplary instructions include instructions for treatment of a bile acid related or associated disorder, such as metabolic syndrome; a lipid- or glucose-related disorder; cholesterol or triglyceride metabolism; type 2 diabetes; cholestasis, including, for example diseases of intrahepatic cholestasis (e.g., PBC, PFIC, PSC, PIC, neonatal cholestasis, and drug induced cholestasis (e.g., estrogen)), and diseases of extrahepatic cholestasis (e.g., bile cut compression from tumor, bile duct blockade by gall stones); bile acid malabsorption and other disorders involving the distal small intestine, including ileal resection, inflammatory bowel diseases (e.g., Crohn's disease and ulcerative colitis), disorders impairing absorption of bile acids not otherwise characterized (idiopathic)) leading to diarrhea (e.g., BAD) and GI symptoms, and GI, liver,
• a kit can contain a collection of such components, e.g. , two or more peptide sequences alone, or a combination of a peptide sequence with another therapeutically useful composition (e.g. , a bile acid homeostasis modulating drug).
• a peptide sequence alone, or a combination of a peptide sequence with another therapeutically useful composition (e.g. , a bile acid homeostasis modulating drug).
• packaging material refers to a physical structure housing the components of the kit.
• the packaging material can maintain the components sterilely, and can be made of material commonly used for such purposes (e.g. , paper, corrugated fiber, glass, plastic, foil, ampules, vials, tubes, etc).
• Kits of the invention can include labels or inserts.
• Labels or inserts include "printed matter," e.g. , paper or cardboard, separate or affixed to a component, a kit or packing material (e.g. , a box), or attached to, for example, an ampule, tube or vial containing a kit component.
• Labels or inserts can additionally include a computer readable medium, such as a disk (e.g. , hard disk, card, memory disk), optical disk such as CD- or DVD-ROM/RAM, DVD, MP3, magnetic tape, or an electrical storage media such as RAM and ROM or hybrids of these such as magnetic/optical storage media, FLASH media or memory type cards.
• Labels or inserts can include identifying information of one or more components therein, dose amounts, clinical pharmacology of the active ingredient(s) including mechanism of action, pharmacokinetics and pharmacodynamics. Labels or inserts can include information identifying manufacturer information, lot numbers, manufacturer location and date.
• Labels or inserts can include information on a condition, disorder, disease or symptom for which a kit component may be used.
• Labels or inserts can include instructions for the clinician or for a subject for using one or more of the kit components in a method, treatment protocol or therapeutic regimen. Instructions can include dosage amounts, frequency or duration, and
• kits of the invention therefore can additionally include labels or instructions for practicing any of the methods and uses of the invention described herein including treatment methods and uses.
• Labels or inserts can include information on any benefit that a component may provide, such as a prophylactic or therapeutic benefit. Labels or inserts can include information on potential adverse side effects, such as warnings to the subject or clinician regarding situations where it would not be appropriate to use a particular composition. Adverse side effects could also occur when the subject has, will be or is currently taking one or more other medications that may be incompatible with the composition, or the subject has, will be or is currently undergoing another treatment protocol or therapeutic regimen which would be incompatible with the composition and, therefore, instructions could include information regarding such incompatibilities.
• Invention kits can additionally include other components. Each component of the kit can be enclosed within an individual container and all of the various containers can be within a single package. Invention kits can be designed for cold storage. Invention kits can further be designed to contain peptide sequences of the invention, or that contain nucleic acids encoding peptide sequences.
• the cells in the kit can be maintained under appropriate storage conditions until ready to use.
• reference to a range of 90-100% includes 91-99%, 92-98%, 93-95%, 91-98%, 91-97%, 91-96%, 91-95%, 91-94%, 91-93%, and so forth.
• Reference to a range of 90-100% also includes 91%, 92%, 93%, 94%, 95%, 95%, 97%, etc., as well as 91.1%, 91.2%, 91.3%, 91.4%, 91.5%, etc., 92.1%, 92.2%, 92.3%, 92.4%, 92.5%, etc., and so forth.
• reference to a range of 1-3, 3-5, 5-10, 10-20, 20-30, 30-40, 40-50, 50-60, 60- 70, 70-80, 80-90, 90-100, 100-1 10, 1 10-120, 120-130, 130-140, 140-150, 150-160, 160-170, 170- 180, 180-190, 190-200, 200-225, 225-250 includes 1 , 2, 3, 4, 5, 6, 7, 8, 9, 10, 1 1 , 12, 13, 14, 15, 16, 17, 18, 19, 20, etc.
• reference to a range of 25-250, 250-500, 500-1000, 1000- 2500 or 2500-5000, 5000-25,000, 5000-50,000 includes any numerical value or range within or encompassing such values, e.g. , 25, 26, 27, 28, 29. ..250, 251 , 252, 253, 254. ...500, 501 , 502, 503, 504. .., etc.
• the invention is generally disclosed herein using affirmative language to describe the numerous embodiments.
• the invention also specifically includes embodiments in which particular subject matter is excluded, in full or in part, such as substances or materials, method steps and conditions, protocols, procedures, assays or analysis.
• the invention is generally not expressed herein in terms of what the invention does not include, aspects that are not expressly included in the invention are nevertheless disclosed herein.
• mice Animals, db/db mice were purchased from The Jackson Laboratory (Bar Harbor, ME), Mice were kept in accordance with welfare guidelines under controlled light (12 hr light and 12 hr dark cycle, dark 6:30 pm-6:30 am), temperature (22 ⁇ 4°C) and humidity (50% ⁇ 20%) conditions. Mice had free access to water (autoclaved distilled water) and were fed ad libitum on a commercial diet (Harlan Laboratories, Indianapolis, IN, Irradiated 2018 Teklad Global 18% Protein Rodent Diet) containing 17 kcal% fat, 23 kcal% protein and 60 kcal% carbohydrate. All animal studies were approved by the NGM Institutional Animal Care and Use Committee.
• PCR FGF19 ORF was amplified with polymerase chain reaction (PCR) using recombinant DNA (cDNA) prepared from human small intestinal tissue.
• cDNA recombinant DNA
• PCR reagents kits with Phusion® high-fidelity DNA polymerase were purchased from New England BioLabs (F-530L, Ipswich, MA). The following primers were used: forward PCR primer:
• Amplified DNA fragment was digested with restriction enzymes Spe I and Not I (the restriction sites were included in the 5' or 3' PCR primers, respectively) and was then ligated with AAV transgene vectors that had been digested with the same restriction enzymes.
• the vector used for expression contained a selectable marker and an expression cassette composed of a strong eukaryotic promoter 5' of a site for insertion of the cloned coding sequence, followed by a 3' untranslated region and bovine growth hormone polyadenylation tail.
• the expression construct is also flanked by internal terminal repeats at the 5' and 3' ends.
• DMEM Mediatech, Inc. Manassas, VA
• fetal bovine serum 10% fetal bovine serum
• lx antibiotic-antimycotic solution Mediatech, Inc. Manassas, VA
• the cells were plated at 50% density on day 1 in 150 mm cell culture plates and transfected on day 2, using calcium phosphate precipitation method with the following 3 plasmids (20 ⁇ g/plate of each): AAV transgene plasmid, pHelperTM plasmids (Agilent Technologies) and AAV2/9 plasmid (Gao et ah, J. Virol. 78:6381 (2004)).
• the cells were scraped off the plates, pelleted by centrifugation at 3000xg and resuspended in buffer containing 20 mM Tris pH 8.5, 100 mM NaCl and 1 mM MgCl 2 .
• the suspension was frozen in an alcohol dry ice bath and was then thawed in 37°C water bath. The freeze and thaw cycles were repeated three times; Benzonase® (Sigma-aldrich, St. Louis, MO) was added to 50 units/ml; deoxycholate was added to a final concentration of 0.25%).
• Viral particles in the supernatant were purified using a discontinued iodixanal (Sigma-aldrich, St. Louis, MO) gradient as previously described (Zolotukhin S. et al (1999) Gene Ther. 6:973).
• the viral stock was concentrated using Vivaspin® 20 (MW cutoff 100,000 Dalton, Sartorius Stedim Biotech, Aubagne, France) and re-suspended in phosphate-buffered saline (PBS) with 10%> glycerol and stored at -80°C.
• PBS phosphate-buffered saline
• HCC Hepatocellular carcinoma
• Serum FGF19/FGF21/variants exposure level assay Whole blood (about 50 ⁇ /mouse) from mouse tail snips was collected into plain capillary tubes (BD Clay Adams SurePrepTM, Becton Dickenson and Co. Sparks, MD). Serum and blood cells were separated by spinning the tubes in an AutocritTM Ultra 3 (Becton Dickinson and Co. Sparks, MD). FGF19, FGF21 , and variant exposure levels in serum was determined using EIA kits (Biovendor) by following the manufacturer's instructions.
• phosphorylation assay can be performed using purified recombinant proteins.
• FGFR binding assay can be conducted using solid phase ELISA. Briefly, a 96-well plate can be coated with 2 ⁇ g/ml anti- hFc antibody and can be incubated with 1 ⁇ g/ml FGFRl-hFc or FGFR4-hFc. Binding to FGF19 variants in the presence of 1 ⁇ g/ ml soluble ⁇ -klotho and 20 ⁇ g/ml heparin can be detected by biotinylated anti- FGF19 antibodies (0.2 ⁇ g/mL), followed by streptavidin- HRP incubation (100 ng/mL).
• Hep3B cells can be stimulated with FGF19 variants for 10 minutes at 37°C, then can be immediately lysed and assayed for ERK phosphorylation using a commercially available kit from Cis-Bio.
• mice were dosed intraperitoneally with either recombinant FGF19 (0.1 mg/kg; 1 mg/kg; 10 mg/kg) or recombinant FGF21 (0.1 mg/kg; 1 mg/kg; 10 mg/kg).
• FGF19 0.1 mg/kg; 1 mg/kg; 10 mg/kg
• FGF21 0.1 mg/kg; 1 mg/kg; 10 mg/kg
• livers were harvested, RNA was extracted, and cyp7al expression was determined by real-time PCR (QPCR) using GADPH as a normalization control.
• n 3
• cyp7al expression values for the various FGF19 and FGF21 concentrations were compared to mice dosed with PBS vehicle control.
• FGF19 dramatically decreased cyp7al expression in a concentration-dependent manner.
• administration of FGF21 caused a reduction of cyp7al expression, the effect was demonstrably less than that observed with FGF19.
• variant M70 The effect of variant M70 on cyp7al expression in human primary hepatocytes was compared to that of FGF19. As noted in FIG. 2, variant M70 repressed cyp7al expression in an amount comparable to that of FGF19.
• FIG. 5 sets forth the results (IC 50 and Cyp7al (%)) in tabular form. While most FGF19 variants that were evaluated exhibited Cyp7al -inhibiting activity, a few variants ⁇ e.g., M90, M96, M98, M5 and M32) no longer repressed Cyp7al .
• FGF19 variants that retain Cyp7al repression activity can be further evaluated in the HCC assay (or other relevant assay or model) described above to identify variants that might be useful for modulating bile acid metabolism and/or for treating bile acid-related diseases ⁇ e.g., bile acid diarrhea and primary biliary cirrhosis) without causing induction of HCC.
• the figures set forth data for variants that were evaluated in the HCC assay.
• lipid elevating activity can be used as an indicator and/or predictor of HCC formation in animals.
• Table 1 Elevated Triglyceride and Cholesterol in db/db Mice Appears to Positively Correlate With HCC Formation (see SEQ ID NOs:99, 5 and 74 to 81).
• Table 2 Elevated Triglyceride and Cholesterol in db/db Mice Appears to Positively Correlate with HCC Formation (see SEQ ID NOs:99, 100 and 82 to 98).
• Table 4 illustrates the peptide "core sequences" of 35 additional FGF19 variants, denoted
• EPEDLRGHLESDMFSSPLETDSMDPFGLVTGLEAVRSPSFEK (SEQ ID NO: 188) at the C- terminal portion, e.g., following the "TSG" amino acid residues of the core sequence.
• Table 4a (see SEQ ID NOs:99, 100, 5, 9, 8, 12, 10, 13, 15, 14, 43, 6 and 7)
• Table 4c (see SEQ ID NOs:99, 100, 5, 52, 54, to 68, 4, 69, 70 and 53)
• Table 5 illustrates the peptide sequences of additional variants.
• Table 6 illustrates the peptide sequences of 3 FGF19 variants, denoted Ml , M2 and M69. The data clearly show that these three variants have the desired characteristics of glucose lowering activity in db/db mice. These three variants appear to elevate lipids in db/db mice.
• mice were injected with FGF19 or FGF21 in AAV vector. Body weight was recorded 4 weeks after injection.
• Table 7 FGF19 reduces body weight in diet-induced obese mice and in ob/ob mice (sequences correspond to aa 1-29 of SEQ ID NO:99 and aa 1-25 of SEQ ID NO: 100, respectively)
• Table 8 Correlation of body weight and liver tumor formation of FGF19, FGF21 and selected variants in db/db mice (see, e.g., SEQ ID NOs:99, 100, 5, 6, 32, 52 and 69)
• mice were injected (subcutaneously) with M5 (0.1 and 1 mg/kg, s.c.) or FGF19 (1 mg/kg, s.c), or variant M69 (0.1 and 1 mg/kg, s.c.) or FGF19 (1 mg/kg, s.c).
• Plasma glucose levels were measured at 2, 4, 7, and 24 hours after injection.
• the results of variant M5 and variant M69 showed similar glucose lowering effects as wild type FGF19 (data not shown).
• This example sets forth several variant polypeptides and particular characteristics thereof, including the variants' effect on glucose lowering, lipid profile parameters, and HCC formation.
• Table 9 compares data generated for variants M5 (SEQ ID NO:5), M6 (SEQ ID NO: 6) and M50 (SEQ ID NO: 50) with data generated for corresponding variant polypeptides (denoted as M144, M145, and M146, respectively) having N-terminal Arg (R) deletions. Only certain sequence domains for each variant are listed: N-terminal domain, Core, and Sheet-8/Loop- 8/Sheet-9 region.
• ID NO:5 ID NO:5
• ID NO:50 ID NO:50
• ID NO:5 ID NO:5
• ID NO:50 ID NO:50
• Ml 40, M141 and Ml 60 The amino acid sequence for M3 is set forth elsewhere herein, and the amino acid sequences for M139, M140, M141 and M160 are as follows: RPLAFSDAGPHVHYGWGDPIRLRHLYTSGPHGLSSCFLRIRADGWDCARGQSAHSLLEIKA VALRTVAIKGVHSVRYLCMGADGKMQGLLQYSEEDCAFEEEILPDGYNVYRSEKHRLPVSL SSAKQRQLYKNRGFLPLSHFLPMLPMVPEEPEDLRGHLESDMFSSPLETDSMDPFGLVTGLE AVRSPSFEK (M139) (SEQ ID NO: 193);
• FGF1 RPLAFSDAGPHVHYGWGDPI RLRHLYTSG //EEIRPDGYNVY// + + + (aa l-20 of SEQ ID O:99) (aa 21-29 of (aa 102-112 of SEQ
• This example shows that administration of M70 in human patients results in suppression of 7a-hydroxy-4-cholsten-3-one (C4), a marker of bile acid synthesis.
• C4 7a-hydroxy-4-cholsten-3-one
• Study subjects Healthy adults in the age range 18-65 years and with normal body weight (body mass index, BMI 20-35) were enrolled in the study. The study protocol was approved by the Human Research Ethics Committee in Australia, and written informed consent was obtained from each subject. For inclusion in the study each subject had to be in good health determined by no clinically significant findings from medical history, physical exam, 12 lead ECG, clinical laboratory findings, and vital signs at screening. Subjects with history or clinical manifestation of any significant metabolic, allergic, dermatological, hepatic, renal, hematological, pulmonary, cardiovascular, GI, neurological, or psychiatric disorder were excluded from enrollment.
• Study Design The study was a randomized, double-blind, placebo-controlled design. Prescreening of subjects was performed 7-30 days prior to entry, and baseline evaluations were performed before treatment. Each subject was given subcutaneous injection of M70 at doses 3 mg/day in a single bolus dose daily for 7 days. Blood samples were collected into heparinized tubes through an indwelling catheter. Blood samples taken on Day 1 and Day 7 at 4.5 hrs or 24 hrs after administration of M70 or placebo were analyzed. Serum levels of 7a-hydroxy-4-cholesten-3-one (C4) were used to monitor CYP7A1 enzymatic activity (bile acid synthesis). They were analyzed from individual serum samples after sample extraction followed by high-pressure liquid chromatography (HPLC) as described previously (Galman et al. (2003) J Lipid Res. 2003;44(4):859- 66).
• HPLC high-pressure liquid chromatography
• Results The data provided in FIG. 6 show that on days 1 and 7, at both 4.5 hours and 24 hours post-dose, serum levels of C4 were significantly suppressed in the patients, as compared to patients receiving a placebo.
• This example shows activation of mouse FGFR4-P-klotho signaling by FGF19, M3, and M70 in a rat myoblast cell line
• ELK luciferase assay was performed in L6 cells transiently transfected with mouse FGFR4, b-klotho, and reporter constructs containing 5xUAS luciferase and GAL4-DNA- binding domain (DBD) fused to ELK1.
• DBD GAL4-DNA-bind domain
• luciferase activity is regulated by the endogenous phosphorylated extracellular signal-regulated kinase (ERK).
• ERK extracellular signal-regulated kinase
• a cell-based receptor activation assay was used to evaluate the ability of mouse FGFR4 to mediate ligand-dependent signaling in the presence of ⁇ -klotho.
• a rat L6 myoblast cell line which lacks endogenous expression of these proteins, was transfected with DNAs encoding FGFR4 and ⁇ -klotho from mouse, as well as plasmids containing an Elk 1 -dependent chimeric transcription factor-based reporter system.
• Table 11 Co-expression of Mouse FGFR4/p-klotho complex in L6 Cells Potentiates Activation of Intracellular Signaling Pathways by FGF19, M3 and M70.

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