WO2012064096A2 - Nouvelle souche de bacillus subtilis, et application associée pour la prévention de la pourriture des racines des végétaux - Google Patents

Nouvelle souche de bacillus subtilis, et application associée pour la prévention de la pourriture des racines des végétaux Download PDF

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WO2012064096A2
WO2012064096A2 PCT/KR2011/008496 KR2011008496W WO2012064096A2 WO 2012064096 A2 WO2012064096 A2 WO 2012064096A2 KR 2011008496 W KR2011008496 W KR 2011008496W WO 2012064096 A2 WO2012064096 A2 WO 2012064096A2
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root rot
ginseng
composition
strain
bacillus subtilis
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PCT/KR2011/008496
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Korean (ko)
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WO2012064096A3 (fr
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김영국
김중수
김형진
배경숙
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한국생명공학연구원
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Priority to CN201180059724.8A priority Critical patent/CN103703121A/zh
Publication of WO2012064096A2 publication Critical patent/WO2012064096A2/fr
Publication of WO2012064096A3 publication Critical patent/WO2012064096A3/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus

Definitions

  • the present invention relates to a novel Bacillus subtilis strain, more specifically Bacillus subtilis strain B-5604, which inhibits the growth of root rot pathogens, the composition for the control of the root rot pathogens comprising the strain or its culture solution, and It relates to a method for controlling the ginseng root rot pathogens using the composition and to inhibit the growth of Sirindrocarpon destructans or Fusarium solani using the strain.
  • Ginseng root rot disease control research has been tried by various researchers. However, the mechanism of pathogenesis of root rot disease caused by syringrocapon destructans and the physiology and ecological basic research of pathogens are insufficient. There is no way to control it. In addition, the root rot was identified as the root rot pathogen in the soil, but after the onset of the effective control means, the root rot caused after ginseng transplantation only prevented the spread of the surrounding environment. In ginseng cultivation, harvesting ginseng and cultivating other crops for 7-10 years, then cultivating ginseng again, and cultivating ginseng, harvesting ginseng, and cultivating ginseng again for 1-2 years without growing other crops. It is tilling.
  • Ginseng is a representative crop that causes obstacles caused by crops.
  • the main ginseng producing area there is an absolute shortage of cropland due to the increase of the required growing area. .
  • the production and quality of ginseng deteriorated due to the lack of adaptation due to the increase of production cost, neglect of management and the change of weather and soil conditions in new areas.
  • root rot occurs when the ginseng package becomes dark brown and leaves become prematurely reddened.
  • Ginseng cultivators recognize that the initial level of root rot pathogens in cropland is not so high that the initial root rot disease occurs at least 50% when harvested for at least 6 years. In general, it is thought to be part of the 50% that is lost during cultivation. However, in the field of serial disturbance, initial colonization rate of root rot disease reached 30% and severe cases reached 80% within 1-2 years after transplantation.
  • Root rot pathogens are inoculated at 30cm from the soil surface and ginseng is transplanted, but root rot disease occurs when the root rot pathogens are inoculated at 50 cm or less. Although a little expected, if the roots rot by root rot pathogens during ginseng cultivation, in the state that the ginseng is being cultivated, it is difficult to use the fumigant because it is impossible.
  • the present inventors have made diligent efforts to find new fungicides that can control environmentally-friendly and low-toxic root-mixing diseases, and as a result, ginseng root rot-inducing microorganisms from environmentally-friendly microbial resources for controlling root rot caused during the planting and re-production of ginseng.
  • Newly developed antagonistic microorganisms to control the present invention was completed.
  • the active antagonistic microorganisms developed in the present invention have no environmental pollution because they are friendly to water quality and soil, and in particular, the active antagonist microorganisms are innovative for ginseng cultivation because they improve the root rot disease of the ginseng under cultivation or re-cultivation of ginseng. It is expected to contribute.
  • One object of the present invention is to provide a novel Bacillus subtilis strain B-5604 (Accession Number: KCTC 11758BP).
  • Another object of the present invention is to provide a composition for controlling root rot pathogens comprising the strain or its culture as an active ingredient.
  • Another object of the present invention to provide a method for controlling the ginseng root rot pathogens using the composition.
  • Another object of the present invention is to use the strain strains of Syringrocarpon destructans ( Cylindrocarpon destructans ) or Fusarium Solani ( Fusarium solani It is to provide a method of suppressing the growth of).
  • Still another object of the present invention is to provide a method for controlling root rot pathogens of the strain or composition.
  • the antagonistic microorganisms developed in the present invention have a new mechanism of action by making a search system that can search for active substances for bactericidal activity of the new mechanism of action by identifying that there is a bactericidal resistance mechanism specific to ginseng root rot disease. It may be used as an active microorganism capable of overcoming the mechanism of bactericidal resistance.
  • it does not cause environmental pollution to ginseng cultivation and reduces the management cost and environmental pollution of domestic ginseng cultivating farmers by supplying eco-friendly ginseng root rot control or pesticide that is not toxic to human body. It will also contribute to the stable supply and export of high-quality ginseng products with high competitiveness.
  • Fusarium Solani strain is known to cause problems not only in American ginseng but also Japanese ginseng
  • the secured active microorganisms are expected to enhance the national competitiveness and contribute to the vitalization of the national economy.
  • 1 is a diagram showing an antagonistic strain showing inhibitory activity on Cylindrocarpon destructans .
  • Figure 2 is a diagram showing the sequence of the gene rDNA encoding the 16S rRNA of Bacillus subtilis B-5604 of the present invention (SEQ ID NO: 1).
  • Figure 3 is an electron micrograph of the antagonist microorganism of the present invention.
  • Figure 4 is a diagram showing the results of plant root rot disease prevention of antagonism microorganisms.
  • 5 is a view showing the results according to the treatment of antagonism microorganisms in plants.
  • the present invention provides a novel Bacillus subtilis strain B-5604 (Accession Number: KCTC 11758BP).
  • the strain is characterized in that it inhibits the growth of root rot pathogens, preferably can inhibit the growth of ginseng root rot pathogens, and thereby can control the ginseng root rot disease that has emerged as a major problem in cultivation of ginseng. have.
  • the term "ginseng root rot” is a soil plant disease that occurs more than 50% during cultivation of ginseng roots is a disease in which the roots rot to dark brown and leaves become prematurely reddened.
  • the bacillus subtilis strain B-5604 a novel strain developed by the present inventors, is a strain of ginseng root rot pathogen, which is a kind of ginseng root rot pathogen. ( Cylindrocarpon destructans ) or Fusarium Solani ( Fusarium solani It was first identified that ginseng root rot can be prevented by suppressing the growth of the.
  • the strain of the present invention is a strain having an antagonistic ability to inhibit the growth of the root rot causing strains, preferably can suppress the growth of the strain causing ginseng root rot.
  • "ginseng root rot pathogen” includes a strain that can cause ginseng root rot, without limitation, for example, phytopsorra cockroach ( Phytophthora cactorum ) , Cylindro car phone destruction tanks ( Cylindrocarpon destructans ) , Fusarium Solani ( Fusarium solani And the like, and preferably, the pathogen which can inhibit growth of the strain of the present invention is Sirandrocarpon destructans.
  • the term “cylindrocaphone destructives” Cylindrocarpon destructans) "is a destructans (scavenger) squalton ( Cylindrocarpon destructans (Zinssm.) Scholten) can be used interchangeably.
  • the pathogen is known as a causative agent of ginseng consecutive disorders, it has been found to form a thick film spores, causing the disease infectious soil.
  • the strain was isolated from the typical black lesion tissue of the root rot disease, pathogenicity was confirmed, and if the growth of the pathogen is inhibited, ginseng root rot disease can be controlled soon.
  • Fusarium solani is a bacterium known as a pathogen of ginseng root rot, and when infected with the fungus, the surface of the ginseng root becomes light brown or reddish brown and soft to the root. do.
  • the present inventors isolated the mutation-induced antagonistic strains by exposure to ultraviolet rays while searching for strains showing inhibitory activity against the Fusarium solanie strain and the ginseng root rot strain of ginseng root rot. Were separated from soil samples (FIG. 1).
  • the sequence of the gene (rDNA) encoding the 16S rRNA of the strain provided by the present invention is set forth in SEQ ID NO: 1 (FIG. 2).
  • the sequence of the rDNA set forth in SEQ ID NO: 1 shows 99% similarity to the known Bacillus subtilis strain AB017589 and 99% identity to another known Bacillus subtilis strain AB017590 Indicated.
  • the size of the strains provided by the present invention was on average 0.6-0.7 X 1.5-1.6 ⁇ m, slightly smaller than the general Bacillus subtilis size of 1.2-2.5 ⁇ m, with one flagella, and its length was approximately 7.5 ⁇ m. Was about 5 times (Fig. 3).
  • Bacillus subtilis As a result of comparing the classification position of the strain, it was classified as Bacillus subtilis, and the strain of the present invention was named "Bacillus subtilis B-5604" based on the characteristics of fatty acid components and ratios, cell appearance, and the like.
  • Bacillus subtilis B-5604 On September 13, 2010, it was deposited with the accession number KCTC 11758BP at the Korea Research Institute of Bioscience and Biotechnology, Korea Institute of Bioscience and Biotechnology, 111, Gwahak-ro, Yuseong-gu, Daejeon, Korea.
  • the strain of the present invention inhibits the growth of Fusarium solani and Sirandrocarpon destructans (see Example 4).
  • the strain of the present invention has a ginseng root rot inhibitory effect, three quarters of the ginseng roots are covered with soil containing the root rot pathogen, and the above is adsorbed to the vermiculite, etc.
  • the soil part where root rot pathogen was present was rotted as it was, but the part grown at the drug attachment part adsorbed to the vermiculite such as the root rot antagonist microorganism was improved and the root rot disease was improved. It confirmed that the state was favorable (FIG. 4).
  • the novel Bacillus subtilis strain B-5604 of the present invention has excellent antagonistic ability against ginseng root rot pathogen, and can be used as an environment-friendly ginseng root rot pathogen control microorganism because there is no environmental pollution and human toxicity. have.
  • the present invention provides a composition for the control of root rot pathogens comprising Bacillus subtilis strain B-5604 (Accession Number: KCTC 11758BP) or a culture thereof as an active ingredient.
  • Root rot pathogens are as described above.
  • compositions of the present invention may be formulated in the form of hydrates, granules or encapsulations for the purpose of stable formulation of microorganisms.
  • the microorganism or the culture medium may be supplied as contained in the composition for controlling ginseng root rot pathogen, or may be stored separately for long-term storage and mixed before use.
  • the microorganisms may be supplied separately for long term storage, they may be stored at -70 ° C. or lower in glycerol stock solution or may be used by freeze-drying at -20 to -80 ° C.
  • the hydrating agent of the present invention may be prepared by drying and grinding a solid medium inoculated with microorganisms, followed by mixing by adding a surfactant and an extender / nutrient.
  • a surfactant polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene Group consisting of alkyl aryl phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers, sodium sulfonate naphthalene formaldehyde, Triton 100 and Tween 80 One or two or more selected from among them may be used, and one or two or more selected from the
  • the granules of the present invention may be prepared by drying and grinding a solid medium inoculated with microorganisms and then adding a surfactant, an extender / nutrient and a disintegrant.
  • a surfactant polycarboxylate, sodium lignosulfonate, calcium lignosulfonate, sodium dialkyl sulfosuccinate, sodium alkyl aryl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene Group consisting of alkyl aryl phosphoric esters, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl aryl polymers, polyoxyalkylone alkyl phenyl ethers, polyoxyethylene nonyl phenyl ethers, sodium sulfonate naphthalene formaldehyde, triton 100 and twin 80 One or two or more selected from the group consisting of one or two or more selected
  • the granules of the present invention may include one or two or more selected from the group consisting of surface active agents, inert carriers, preservatives, wetting agents, feed promoters, attractants, encapsulating agents, binders, emulsifiers, dyes, UV protectors, buffers and flow agents for microorganisms. It can be prepared by adding additional.
  • the composition can be applied to the root of the plant or the soil around it to control the root rot pathogen. More preferably, it can be applied to the roots of or around the ginseng to control the ginseng root rot pathogens.
  • the method of applying the composition may be directly spreading, spraying, soaking the culture or strain of the strain, or scattering or cultivating the strain culture or the strain by adsorbing on vermiculite, diatomaceous earth, perlite, or tilling.
  • a method of mixing the strain culture medium or the adsorbed strains during soil plowing can be used.
  • known pesticide administration methods, microbial application methods and the like can be used.
  • composition is sprayed by concentrating Bacillus subtilis B-5604 in the roots or adjacent soil of ginseng, the effective amount of microorganisms is 1 X 10 7 to 5 X 10 7 May be sparged, preferably 4 ⁇ 10 7 birds.
  • the application of microorganisms can be achieved by spraying the roots of ginseng with a solution in which the effective amount of microorganisms contained in the composition is diluted at a concentration of 1 X 10 6 to 1 X 10 9 microorganisms per ml (ml), or per ml (ml) Ginseng may be immersed in a solution diluted to a concentration of 1 X 10 6 to 1 X 10 9 microorganisms for 1 to 2 hours.
  • the present invention is a method for controlling ginseng root rot pathogens using the composition for the control of root rot pathogens comprising Bacillus subtilis strain B-5604 (Accession Number: KCTC 11758BP) or a culture thereof as an active ingredient.
  • KCTC 11758BP Bacillus subtilis strain B-5604
  • the method of controlling the ginseng root rot pathogens can be controlled by spraying or immersing the composition in the root or the soil adjacent to the ginseng, or immersed, the details are as described above.
  • composition can inhibit the growth of ginseng root rot pathogens to control the ginseng root rot pathogens, the description of the ginseng root rot pathogens is as described above.
  • the present invention provides a Cylindrocarpon distractans using Bacillus subtilis strain B-5604 (Accession Number: KCTC 11758BP). ( Cylindrocarpon destructans ) or Fusarium Solani ( Fusarium solani It provides a method of suppressing the growth of).
  • strains, strains that are subject to growth inhibition and growth methods are as described above.
  • the present invention comprises Bacillus subtilis strain B-5604 (Accession No .: KCTC 11758BP), or Bacillus subtilis strain B-5604 (Accession No .: KCTC 11758BP) or its culture solution as an active ingredient.
  • Bacillus subtilis strain B-5604 accesion No .: KCTC 11758BP
  • Bacillus subtilis strain B-5604 accesion No .: KCTC 11758BP
  • its culture solution as an active ingredient.
  • Provided is a method for controlling root rot pathogens of the composition for root rot pathogen control.
  • Control uses may be carried out by controlling the root rot pathogens by spraying or soaking the strain or composition concentrated on the root of the plant or the soil adjacent thereto, and the details are as described above.
  • the plant may be ginseng, thereby controlling the ginseng root rot pathogens.
  • the strain or composition may control the growth of the root rot pathogens by inhibiting the growth of the root rot pathogens, the description of the root rot pathogens as described above.
  • the present inventors searched for antagonistic microorganisms among microorganisms that can also grow in the inhibitory activity produced by the root rot pathogens in the soil samples of Chungcheongnam-do in order to search for antagonistic microorganisms that inhibit the growth of root rot pathogens of ginseng.
  • Investigation of antagonistic microorganisms that inhibit the growth of root rot pathogens of ginseng is carried out by diluting the soil sample in distilled water stepwise to spread the inhibitory substances produced by the root rot pathogens on a separation medium, and then selecting and separating candidate strains. was performed.
  • the microorganism separation medium used by the present inventors (yeast extract 0.1%, polypeptone 0.5%, potassium monohydrogen phosphate 0.05%, potassium diphosphate 0.05%, magnesium sulfate seven times crystallized water 0.02%, agar 1.5%, hydrogen ion concentration 7.2) After sterilization, the temperature was lowered to about 50 ° C, and a solution of colistin dissolved in distilled water at a concentration of 5 mg / L as an added antibiotic and 10 mg / of nalidixic acid was added to suppress growth of general bacteria. 1 ml per 1 liter of the sterilized separation medium in which the solution dissolved in methanol at a concentration of l was mixed well and then a solidified separation medium was used.
  • the search for antagonistic microorganisms was selected from microorganisms capable of growing even on antibiotics with inhibitory activity produced by root rot pathogens. Searching for antagonistic microorganisms primarily inhibits the growth of Fusarium Solani strains by first separating the selected microorganisms and spreading the strains to be searched during the cultivation of Fusarium Solani strains, the root rot-causing bacteria, on agar medium per starch. Antagonistic microorganisms with this were selected secondarily.
  • LBG tryptone 1.0%, yeast extract 0.5%, NaCl 1.0%, glucose 1.06%
  • Selected strains were cultured in LB liquid medium and then produced medium (sucrose 30g, soybean meal 20G, MnCl 2 4H 2 O 5mg, FeSO 4 7H 2 Strains with increased inhibitory activity compared to the parent strain were selected from the high titer test specimens of Syringrocarpon destructhans by incubating for 24 to 48 hours in O 50mg per liter).
  • the sequence of the gene (rDNA) encoding 16S rRNA of the antagonistic microbial strain selected in Example 1 is shown in Figure 2 (SEQ ID NO: 1).
  • Figure 2 The sequence of the gene (rDNA) encoding 16S rRNA of the antagonistic microbial strain selected in Example 1 is shown in Figure 2 (SEQ ID NO: 1).
  • the rDNA sequences of Bacillus subtilis selected in Example 1 described in SEQ ID NO: 1 are known from Bacillus subtilis strains AB017589 and 99 % Similarity and 99% concordance with another known Bacillus subtilis strain AB017590.
  • the size of the strain was 0.6-0.7 X 1.5-1.6 ⁇ m on average
  • the size of the general Bacillus subtilis was slightly smaller than 1.2-2.5 ⁇ m, with one flagella having a length of approximately 7.5 ⁇ m, which was about 5 times the size of the cell (FIG. 3).
  • PCR was performed using two kinds of primers to amplify the gene, and PCR conditions and sequencing were analyzed by Chun and Goodfellow methods. Accordingly. As a result, the new strains were classified into Bacillus subtilis based on the comparison data of 16S rDNA sequences of the microorganisms most used to identify the location of the new strains. Based on the characteristics of the strain selected in Example 1 of the present invention was named a new strain "Bacillus subtilis B-5604", on September 13, 2010 Korea Biotechnology Research Institute Biological Resource Center (Daejeon, Korea) It was deposited with Accession No. KCTC 11758BP in Yuseong-gu Hagyuro-ro 111).
  • Bacillus subtilis B-5604 an antagonistic microorganism with inhibitory activity against ginseng root rot, was cultured by shaking at 28 ° C for 24 hours in LB medium (1% casein peptone, 0.5% yeast extract, 0.5% salt, after autoclaving). And 20% glycerin were mixed at a ratio of 1: 1 and placed in frozen vials, each of which was used for culturing the active material while being frozen at -70 ° C.
  • the frozen Bacillus subtilis B-5604 vial is dissolved and the active substance production medium (1% soluble starch, 0.5% Pharmamade 0.5%) in a 1-liter Erlenmeyer flask.
  • the active substance production medium 1% soluble starch, 0.5% Pharmamade 0.5%) in a 1-liter Erlenmeyer flask.
  • Shake culture was performed at a speed of 200 rpm.
  • the foam defoaming agent produced at the time of culture used an appropriate amount of silicone oil.
  • Bacillus subtilis Bacillus subtilis Strain B-5604 is Fusarium Solani or Cylindrocarpon distractans To confirm the fact that it inhibits the growth of strains, Fusarium Solani or Syringrocarpon destructans in PDA medium The growth inhibition activity of the strain was analyzed. Fusarium solani or cyndrocarpon distractans incubated for 72 hours at 37 ° C. in PDA medium The strain was cultivated 3X3 mm with sterile spatula and placed on PDA medium, and Bacillus subtilis strain B-5604 was taken with sterile white gold teeth, side-by-side and incubated for 28 hours at 28 ° C. Leeum Solani or Cylindrocaphone Distantans It was confirmed that the growth of the strain is suppressed.
  • the presence of root rot pathogens was confirmed in the ginseng arable land at the time of the test in May, and 2 years old ginseng was planted in 1 field out of 5000 (Note: the size of one cell is 1.8x 0.9 m and the ginseng used for the test)
  • the number of ginseng roots was transplanted to 500 roots per year, and three quarters of the ginseng roots were covered with soil containing the root rot pathogen, and the same amount of vermiculite as the root rot control antagonism microorganism cultured by the method of Example 3 was placed thereon.
  • Ginseng was harvested in August of the same year by covering the dried soil with adsorption on the back.
  • the root rot inhibiting experiment of antagonistic microorganisms confirmed the presence of root rot pathogens in the ginseng farmland and transplanted 2 year old ginseng to 1 of 5000 ginseng ginseng planted in the cultivated land, and cultivated the control covered with soil containing root rot pathogens. .
  • the test group transplanted 2 years old ginseng and cultivated the root rot disease control antagonist active microorganism cultured by the method of Example 3 to adsorb the same amount of vermiculite, and then mixed the dried vermiculite with soil present in the root rot pathogen 1: 1
  • ginseng was harvested to investigate the improvement of root rot of ginseng against control and root rot control antagonist microorganisms.

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Abstract

La présente invention concerne une nouvelle souche de Bacillus subtilis, et plus particulièrement, la souche B-5604 de Bacillus subtilis, qui inhibe la croissance des pathogènes responsables de la pourriture des racines, une composition contenant ladite souche ou son milieu de culture pour lutter contre les pathogènes responsables de la pourriture des racines, un procédé pour lutter contre les pathogènes responsables de la pourriture des racines du ginseng à l'aide de ladite composition, et un procédé permettant d'inhiber la croissance de Cylindrocarpon destructans ou de Fusarium solani à l'aide de ladite souche. Les microorganismes ayant une activité antagoniste conçus selon la présente invention ne sont pas nocifs pour l'eau ni pour la terre, et n'entraînent donc aucune pollution de l'environnement, et particulièrement, les microorganismes ayant une activité antagoniste selon la présente invention peuvent non seulement réduire les pathogènes responsables de la pourriture des racines dans les terres où le ginseng est cultivé ou recultivé de façon répétée, mais peuvent également soulager la pourriture des racines du ginseng cultivé, contribuant ainsi de façon significative à la culture du ginseng.
PCT/KR2011/008496 2010-11-09 2011-11-09 Nouvelle souche de bacillus subtilis, et application associée pour la prévention de la pourriture des racines des végétaux WO2012064096A2 (fr)

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Cited By (8)

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CN102805107A (zh) * 2012-05-30 2012-12-05 山东京青农业科技有限公司 一种防治苹果轮纹腐烂病的微生物制剂及应用
WO2016127184A1 (fr) * 2015-02-06 2016-08-11 University Of Florida Researchfoundation, Inc. Bactéries et procédé permettant d'améliorer la santé et la croissance de plantes
US9622484B2 (en) 2014-12-29 2017-04-18 Fmc Corporation Microbial compositions and methods of use for benefiting plant growth and treating plant disease
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CN110643540A (zh) * 2019-10-23 2020-01-03 莲花县兴木农业科技有限公司 一种防治根腐病的药物菌剂的制备方法及其应用
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CN113303340A (zh) * 2021-05-21 2021-08-27 宁夏大学 生防细菌和鱼蛋白的组合物及其在黄瓜栽培中的应用
CN113264803A (zh) * 2021-06-28 2021-08-17 厦门市江平生物基质技术股份有限公司 太子参根腐病连作障碍的土壤修复基质及其应用

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