WO2012019326A8 - 一种农杆菌介导的小油桐基因转化方法 - Google Patents

一种农杆菌介导的小油桐基因转化方法 Download PDF

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WO2012019326A8
WO2012019326A8 PCT/CN2010/001376 CN2010001376W WO2012019326A8 WO 2012019326 A8 WO2012019326 A8 WO 2012019326A8 CN 2010001376 W CN2010001376 W CN 2010001376W WO 2012019326 A8 WO2012019326 A8 WO 2012019326A8
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agrobacterium tumefaciens
jatropha curcas
mediated gene
transformation method
gene transformation
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PCT/CN2010/001376
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English (en)
French (fr)
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WO2012019326A1 (zh
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徐增富
潘竟丽
付乾堂
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中国科学院西双版纳热带植物园
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Priority to BR112013003362A priority Critical patent/BR112013003362A2/pt
Publication of WO2012019326A1 publication Critical patent/WO2012019326A1/zh
Publication of WO2012019326A8 publication Critical patent/WO2012019326A8/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

提供一种农杆菌介导的小油桐基因转化方法，包括步骤：将小油桐种子的子叶切除其基部的1/3-1/4后作为外植体用于农杆菌感染，子叶外植体与农杆菌共培养后，转移至无筛选剂的培养基上诱导愈伤组织共4周，继代到以硫酸卡那霉素为筛选剂的选择培养基上筛选抗性芽，继代培养抗性芽获得抗性植株，经分子检测试验验证转基因植株。
PCT/CN2010/001376 2010-08-13 2010-09-08 一种农杆菌介导的小油桐基因转化方法 WO2012019326A1 (zh)

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BR112013003362A BR112013003362A2 (pt) 2010-08-13 2010-09-08 método de transformação genética mediada por uma agrobacterium tumefaciens para jatropha curcas

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CN201010252415.X 2010-08-13
CN 201010252415 CN101948867B (zh) 2010-08-13 2010-08-13 一种农杆菌介导的小油桐基因转化方法

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WO2012019326A1 WO2012019326A1 (zh) 2012-02-16
WO2012019326A8 true WO2012019326A8 (zh) 2013-02-14

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CN102304543B (zh) * 2011-08-18 2014-05-28 普罗米绿色能源(深圳)有限公司 利用卡那霉素抗性基因作为筛选标记对麻疯树进行基因转化的方法
CN102994544A (zh) * 2012-12-04 2013-03-27 浙江大学 农杆菌介导转基因培养大岩桐植株的方法
CN103385168B (zh) * 2013-07-01 2014-08-20 中南林业科技大学 一种油桐叶片再生植株的方法
CN104094848B (zh) * 2014-07-10 2015-09-09 中南林业科技大学 油桐下胚轴愈伤组织诱导及高效再生植株的方法
CN106636196B (zh) * 2017-02-21 2019-11-19 辽宁省农业科学院大连生物技术研究所 一种优化的、高效率的农杆菌介导的花生转化方法
CN109874450B (zh) * 2019-01-31 2023-08-01 浙江大学 一种高通量筛选转基因纯合植株的装置及方法
CN110747196A (zh) * 2019-10-16 2020-02-04 中国科学院西双版纳热带植物园 一种在植物花中表达的组织特异性启动子JcTM6基因启动子及其应用
CN111500622A (zh) * 2020-04-02 2020-08-07 山西师范大学 一种二穗短柄草遗传转化方法
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CN113584072B (zh) * 2021-07-29 2024-02-06 上海市农业科学院 草莓的遗传转化体系构建方法
CN113832178A (zh) * 2021-09-08 2021-12-24 浙江省农业科学院 一种发根农杆菌介导的菜用豌豆遗传转化体系建立的方法
CN114107376A (zh) * 2021-12-17 2022-03-01 临沂大学 一种烟草的农杆菌遗传转化方法
CN114592001A (zh) * 2022-03-01 2022-06-07 华中农业大学 一种不依赖基因型的除虫菊遗传转化方法
CN114410679B (zh) * 2022-03-10 2024-01-09 中国林业科学研究院亚热带林业研究所 山鸡椒的遗传转化方法和应用
CN114657206B (zh) * 2022-03-17 2024-03-22 中国热带农业科学院热带作物品种资源研究所 一种柱花草的遗传转化方法
CN114568309A (zh) * 2022-04-01 2022-06-03 北京大学现代农业研究院 花生组培方法和花生遗传转化方法
CN114807219B (zh) * 2022-04-15 2024-02-09 河南省农业科学院芝麻研究中心 一种农杆菌介导的芝麻子叶转基因方法
CN114958898B (zh) * 2022-05-20 2023-11-14 中国农业科学院郑州果树研究所 Peg介导的菜豆壳球孢遗传转化体系的建立方法及其应用
CN114836468B (zh) * 2022-05-25 2023-07-28 东北林业大学 一种白桦根转基因方法
CN114921491B (zh) * 2022-06-15 2023-07-14 沈阳农业大学 一种农杆菌介导的芍药瞬时表达的方法
CN115074383B (zh) * 2022-07-22 2023-05-23 中国科学院武汉植物园 一种发根农杆菌介导的桃愈伤组织遗传转化方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1149918C (zh) * 2001-02-26 2004-05-19 山西省农业生物技术研究中心 农杆菌介导植物萌发种子基因转化方法

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CN101948867B (zh) 2012-06-27
CN101948867A (zh) 2011-01-19
BR112013003362A2 (pt) 2016-07-12
WO2012019326A1 (zh) 2012-02-16

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