WO2011074273A1 - Dispositif d'analyse automatique - Google Patents

Dispositif d'analyse automatique Download PDF

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Publication number
WO2011074273A1
WO2011074273A1 PCT/JP2010/007350 JP2010007350W WO2011074273A1 WO 2011074273 A1 WO2011074273 A1 WO 2011074273A1 JP 2010007350 W JP2010007350 W JP 2010007350W WO 2011074273 A1 WO2011074273 A1 WO 2011074273A1
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WO
WIPO (PCT)
Prior art keywords
sample
container
dispensing
dispensing probe
liquid level
Prior art date
Application number
PCT/JP2010/007350
Other languages
English (en)
Japanese (ja)
Inventor
三島直子
Original Assignee
ベックマン コールター， インコーポレイテッド
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Application filed by ベックマン コールター， インコーポレイテッド filed Critical ベックマン コールター， インコーポレイテッド
Publication of WO2011074273A1 publication Critical patent/WO2011074273A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B01F31/651—Mixing by successively aspirating a part of the mixture in a conduit, e.g. a piston, and reinjecting it through the same conduit into the receptacle
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N35/1011—Control of the position or alignment of the transfer device
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1048—General features of the devices using the transfer device for another function
    • G01N2035/1058—General features of the devices using the transfer device for another function for mixing
    • G01N2035/106—General features of the devices using the transfer device for another function for mixing by sucking and blowing

Definitions

  • the present invention relates to an automatic analyzer, and a sample stirring method and a sample dispensing method in the automatic analyzer.
  • an automatic analyzer for analyzing a sample such as blood or body fluid dispenses a predetermined amount of a sample from a sample container containing the sample into a reaction container, and causes the sample to react with the reagent in the reaction container. Analyzing.
  • a sample container containing the sample into a reaction container
  • HbA1c hemoglobin A1c
  • the nozzle descending distance is calculated from the liquid surface position information and the sample container information of the whole blood sample contained in the sample container, and is calculated
  • an automatic analyzer that dispenses blood cell components from a specimen in which blood cells have settled by operating a specimen dispensing mechanism based on the nozzle descending distance (see Patent Documents 1 and 2).
  • the type of the sample container is determined by storing the blood cell sample aspiration position for each type of sample container.
  • An analyzer that aspirates a blood cell sample at a stored suction position for each sample container is disclosed (see Patent Document 3).
  • the reagent into the separated plasma / blood specimen is a position where the reagent probe can reach the blood cell layer.
  • the red blood cell layer is aspirated with the reagent probe, and the aspirated specimen is discharged as it is in the specimen, and the specimen is agitated.
  • An automatic analyzer that dispenses a sample has been proposed (see Patent Document 4).
  • the present invention relates to a sample container storage unit that stores a sample container for storing a sample, a reagent container storage unit that stores a reagent container for a reagent used for analyzing the sample, and a reaction between the reagents.
  • a sample-dispensing unit having a reaction-container storing unit for storing a reaction container for dispensing the sample, and a dispensing probe for dispensing the sample stored in the sample-container.
  • Sample dispensing means for dispensing a sample
  • liquid level detecting means for detecting the liquid level of the sample contained in the sample container
  • dropping the dispensing probe into the sample container to detect the liquid level
  • An agitation control means for controlling the sample to be agitated by repeating aspiration and discharge of the sample a plurality of times by the dispensing probe after reaching the sample liquid level detected by the means in the reaction container. Reagent and anti It is allowed to provide an automatic analyzer for analyzing the specimen.
  • the automatic analyzer of the present invention dispenses the sample into a reaction container by a sample dispensing means having a dispensing probe for dispensing the sample contained in the sample container, and the reagent is contained in the reaction container.
  • the automatic analyzer for analyzing the sample by reacting with the liquid level detecting means for detecting the level of the sample liquid stored in the sample container, the dispensing probe is lowered into the sample container, and the liquid level is detected.
  • agitation control means for controlling the sample to be agitated by repeating aspiration and discharge of the sample a plurality of times by the dispensing probe after reaching the sample liquid level detected by the detection means.
  • the automatic analyzer is the above-described invention, wherein the sample is a blood sample, and the sample information acquisition unit acquires the analysis item of the sample, and the sample information acquisition unit acquires the sample information Determination means for determining whether there is an analysis item using a whole blood sample as a sample analysis item, and when the determination means determines that there is an analysis item using a whole blood sample as a sample,
  • the agitation control means controls the sample to be agitated by repeating aspiration and discharge of the sample a plurality of times by the dispensing probe before dispensing the whole blood sample for the analysis item.
  • the agitation control unit controls the agitation control unit to start agitation by the aspiration and discharge of the specimen by the dispensing probe from the aspiration.
  • the automatic analyzer is the above-described invention, wherein the sample information acquisition unit acquires sample container information including the type of the sample container, and the sample container acquired by the sample information acquisition unit Calculation means for calculating a sample height based on information and a sample liquid level position detected by the liquid level detection means, and the agitation control means calculates the aspiration and discharge of the sample by the dispensing probe. Based on the sample height calculated by the means, control is performed so as to perform at different height positions.
  • the agitation control means sets the initial suction amount of the specimen by the dispensing probe to be larger than the discharge amount.
  • the automatic analyzer according to the present invention is the automatic analyzer according to the invention described above, wherein the stirring control means repeats the suction and discharge of the sample by the dispensing probe, and the dispensing probe is placed in the sample container. Control to descend toward the bottom.
  • the automatic analyzer according to the present invention is the automatic analyzer according to the above invention, wherein the agitation control unit lowers the dispensing probe after the sample is aspirated and discharged by the dispensing probe, and the aspiration is performed. -Control is performed so that the discharge and the lowering are repeated.
  • the automatic analyzer of the present invention is the above-described invention, wherein the sample is a blood sample separated into plasma and blood cells.
  • the automatic analyzer of the present invention is the above-described invention, wherein the determination unit determines whether or not there is an analysis item using a plasma sample as the analysis item of the sample, and the determination
  • the means determines that the analysis item of the sample includes an analysis item having a whole blood sample as a sample and an analysis item having a plasma sample as a sample
  • the order of the analysis items of the sample is changed to the whole blood sample as a sample.
  • the agitation control unit controls the sample to be agitated after the dispensing of the analysis item using the plasma as a sample is completed.
  • the analysis item using the whole blood sample as a sample is HbA1c.
  • the automatic analyzer is the above invention, wherein the calculation unit calculates a sample aspiration position based on the calculated sample height, and the agitation control unit is configured to agitate the sample after agitation.
  • the whole blood sample is aspirated and collected by the dispensing probe at the sample aspiration position calculated by the calculation means.
  • the automated analyzer of the present invention includes any two or more of the features described above.
  • the present invention provides a sample agitation method in an automatic analyzer, and the sample agitation method includes a sample dispensing means having a dispensing probe for dispensing a sample accommodated in a sample container.
  • a sample agitation method in an automatic analyzer for analyzing the sample by reacting with a reagent in the reaction vessel and detecting the liquid level of the sample contained in the sample container. The sample is lowered by the detection step, the dispensing probe is lowered into the sample container, and after reaching the sample liquid level detected in the liquid level detection step, the sample is aspirated and discharged by the dispensing probe repeatedly.
  • a stirring step of stirring is used to stir.
  • the sample agitation method in the automatic analyzer of the present invention is the sample information acquisition step in which the sample is a blood sample and the analysis item of the sample is acquired in the above invention, and the sample information
  • the agitation step is controlled so that the sample is agitated by repeating aspiration and discharge of the sample a plurality of times by the dispensing probe before dispensing the whole blood sample for the analysis item. To do.
  • the sample agitation method in the automatic analyzer of the present invention is the above invention, wherein the agitation step starts agitation by aspiration and discharge of the sample by the dispensing probe from aspiration. Control.
  • the sample agitation method in the automatic analyzer of the present invention is the above invention, wherein the sample information acquisition step acquires sample container information including the type of the sample container, and the sample information acquisition step includes A calculation step of calculating a sample height based on the acquired sample container information and the sample liquid level position detected by the liquid level detection step, wherein the agitation step includes aspiration and discharge of the sample by the dispensing probe. Based on the sample height calculated in the calculation step, control is performed so as to be performed at different height positions.
  • the agitation step sets an initial aspiration amount of the sample by the dispensing probe to be larger than a discharge amount.
  • the sample agitation method in the automatic analyzer of the present invention is the above-described invention, wherein the agitation step is performed while the sample is aspirated and discharged while the sample is aspirated and discharged.
  • the sample container is lowered toward the bottom of the sample container and stirred.
  • the sample agitating method in the automatic analyzer of the present invention is the above invention, wherein the agitating step lowers the dispensing probe after the sample is aspirated and discharged by the dispensing probe. Then, the agitation is performed by repeating the suction / discharge and the lowering.
  • the sample agitation method in the automatic analyzer of the present invention is the above invention, wherein the determination step determines whether or not the analysis item of the sample includes an analysis item using a plasma sample as a sample.
  • the determination step determines that the analysis item of the sample includes an analysis item using the whole blood sample as a sample and an analysis item using the plasma sample as a sample
  • the order of the analysis items of the sample is determined as whole blood Including a changing step of changing the analysis item having the sample as the sample to the last
  • the stirring step controls the stirring of the sample after the dispensing of the analysis item having the plasma as the sample is completed .
  • the sample agitation method in the automatic analyzer of the present invention is the above-described invention
  • the analysis item using the whole blood sample as a sample is HbA1c.
  • the sample agitation method in the automatic analyzer of the present invention is the above-described invention, wherein the sample is put into the reaction container by the sample dispensing means having a dispensing probe for dispensing the sample accommodated in the sample container.
  • a sample dispensing method in an automatic analyzer that dispenses and reacts with a reagent in the reaction vessel to analyze the sample, and the calculation step calculates after the sample is stirred by the sample stirring method described above
  • a specimen aspiration position is calculated based on the specimen height, and the whole blood specimen is aspirated by the dispensing probe at the computed specimen aspiration position, and the aspirated whole blood specimen is discharged into the reaction container.
  • the method of the present invention includes any one or more (two or more) optional features of the automatic analyzer and method of the present invention.
  • the present invention provides a method in which the sample is dispensed into a reaction container by a sample dispensing means having a dispensing probe for dispensing a sample contained in the sample container, and reacted with a reagent in the reaction container.
  • a control mechanism for controlling the stirring of the sample in the automatic analyzer for analyzing the sample, the liquid level detecting means for detecting the sample liquid level stored in the sample container, and the dispensing probe in the sample container Stirring control means for controlling the sample to be stirred by repeating aspiration and discharge of the sample a plurality of times by the dispensing probe after reaching the sample liquid level detected by the liquid level detection means;
  • a control mechanism is provided.
  • control mechanism of the present invention includes any one or more of the above features of the automatic analyzer and method of the present invention.
  • the present invention provides a method in which the sample is dispensed into a reaction container by a sample dispensing means having a dispensing probe for dispensing a sample contained in the sample container, and reacted with a reagent in the reaction container.
  • a control program for controlling the stirring of the sample in the automatic analyzer for analyzing the sample the control program for implementing a process executed by the automatic analyzer according to an instruction from an operator;
  • the processing includes a liquid level detection procedure for detecting the sample liquid level stored in the sample container, and the dispensing probe is lowered into the sample container and reaches the sample liquid level detected by the liquid level detection procedure.
  • a stirring procedure for stirring the sample by repeatedly aspirating and discharging the sample by the dispensing probe.
  • the program of the present invention includes any one or more of the above features of the automatic analyzer, method and control mechanism of the present invention.
  • the present invention provides a method in which the sample is dispensed into a reaction container by a sample dispensing means having a dispensing probe for dispensing a sample contained in the sample container, and reacted with a reagent in the reaction container.
  • a computer-readable recording medium recording a control program for controlling sample agitation in an automatic analyzer for analyzing the sample, wherein the control program is a process executed by the automatic analyzer in accordance with an instruction from an operator
  • the processing includes a liquid level detection procedure for detecting the liquid level of the specimen contained in the specimen container, and the dispensing probe is lowered into the specimen container to detect the liquid level.
  • a stirring procedure in which the sample is stirred by repeating aspiration and discharge of the sample with the dispensing probe after reaching the sample liquid level detected in the procedure. It provides a medium.
  • the recording medium of the present invention includes any one or more of the above features of the automatic analyzer, method, control mechanism, and program of the present invention.
  • the dispensing probe of the sample dispensing unit is lowered into the sample container based on the sample liquid level information detected by the liquid level detection unit, and the sample is aspirated / discharged a plurality of times by the dispensing probe.
  • the sample can be easily agitated, so that it is possible to collect a whole blood sample without performing the work of inversion mixing of the sample container.
  • FIG. 1 is a schematic diagram showing an automatic analyzer according to an embodiment of the present invention.
  • FIG. 2 is a schematic diagram of a sample dispensing apparatus used in the automatic analyzer of FIG.
  • FIG. 3 is a flowchart of the sample dispensing process according to the embodiment of the present invention.
  • FIG. 4 is an operation diagram of whole blood sample dispensing according to the embodiment of the present invention.
  • FIG. 1 is a schematic view showing an automatic analyzer according to an embodiment of the present invention.
  • the automatic analyzer 1 controls a measurement mechanism 9 that measures light passing through a reaction product between a specimen and a reagent, and the entire automatic analyzer 1 including the measurement mechanism 9. And a control mechanism 10 for analyzing a measurement result in the measurement mechanism 9.
  • the automatic analyzer 1 automatically performs analysis of a plurality of samples by cooperation of these two mechanisms.
  • the measurement mechanism 9 roughly includes a sample table 2, a reaction table 3, a reagent table 4, a sample dispensing device 5, a reagent dispensing device 7, and dispensing probe cleaning devices 6 and 8.
  • the sample table 2 includes a disk-shaped table, and includes a plurality of sample container storage portions 21 arranged at equal intervals along the circumferential direction of the table.
  • a sample container 22 containing a sample is detachably stored in each sample container storage unit 21, a sample container 22 containing a sample is detachably stored.
  • the sample container 22 has an opening that opens upward.
  • the sample table 2 is rotated in a direction indicated by an arrow in FIG. 1 by a sample table driving unit (not shown) with a vertical line passing through the center of the sample table 2 as a rotation axis.
  • the sample container 22 is transported to the sample aspirating position where the sample is aspirated by the sample dispensing device 5.
  • the sample table 2 includes a reading unit 23 that reads information on a recording medium of the sample container 22.
  • the reading unit 23 emits infrared light or visible light to the recording medium and processes reflected light from the recording medium to read information on the recording medium.
  • the reaction table 3 has an annular table and includes a plurality of reaction container storage portions 31 arranged at equal intervals along the circumferential direction of the table.
  • a transparent reaction container 32 for storing a sample and a reagent is detachably stored in an open shape upward.
  • the reaction table 3 is rotated in a direction indicated by an arrow in FIG. 1 by a reaction table driving unit (not shown) with a vertical line passing through the center of the reaction table 3 as a rotation axis.
  • the reaction container 32 is transported to the sample discharge position where the sample is discharged by the sample dispensing apparatus 5 or the reagent discharge position where the reagent is discharged by the reagent dispensing apparatus 7.
  • the photometric device 33 has a light source 33a and a light receiving unit 33b.
  • the light source 33a emits analysis light having a predetermined wavelength
  • the light receiving unit 33b measures the intensity of light emitted from the light source 33a and transmitted through the reaction solution in which the specimen and the reagent contained in the reaction container 32 have reacted.
  • the light source 33 a and the light receiving unit 33 b are arranged at positions where the light source 33 a and the light receiving unit 33 b are opposed to each other in the radial direction with the reaction container storage unit 31 of the reaction table 3 interposed therebetween.
  • the reaction table 3 includes a reaction container cleaning device 34 that discharges the measured reaction solution from the reaction container 32 and cleans the reaction container 32.
  • the reagent table 4 has a disk-shaped table and includes a plurality of reagent container storage portions 41 arranged at equal intervals along the circumferential direction of the table.
  • a reagent container 42 containing a reagent is detachably stored in each reagent container storage unit 41.
  • the reagent container 42 has an opening that opens upward.
  • the reagent table 4 is rotated in a direction indicated by an arrow in FIG. 1 by a reagent table driving unit (not shown) with a vertical line passing through the center of the reagent table 4 as a rotation axis.
  • the reagent container 42 is transported to the reagent suction position where the reagent is sucked by the reagent dispensing device 7.
  • the reagent table 4 includes a reading unit 43 that reads information on the recording medium of the reagent container 42.
  • the reading unit 43 emits infrared light or visible light to the recording medium, and reads the information on the recording medium by processing the reflected light from the recording medium.
  • the sample dispensing device 5 has a dispensing probe for aspirating and discharging a sample attached to the distal end, and freely moves up and down in the vertical direction and rotates around a vertical line passing through its proximal end as a central axis. Provide an arm.
  • the sample dispensing device 5 is provided between the sample table 2 and the reaction table 3, sucks the sample in the sample container 22 transported to a predetermined position by the sample table 2 with a dispensing probe, rotates the arm, The sample is dispensed into the reaction container 32 conveyed to a predetermined position by the reaction table 3, and the sample is transferred into the reaction container 32 on the reaction table 3 at a predetermined timing.
  • the sample dispensing device 5 includes a liquid level detection unit 60 (see FIG. 2) that detects the sample liquid level stored in the sample container 22.
  • the reagent dispensing apparatus 7 has a dispensing probe for aspirating and discharging the reagent attached to the distal end portion, and freely moves up and down in the vertical direction and rotates around the vertical line passing through its base end portion as a central axis. Provide an arm.
  • the reagent dispensing device 7 is provided between the reagent table 4 and the reaction table 3, sucks the reagent in the reagent container 42 transported to a predetermined position by the reagent table 4 with a dispensing probe, rotates the arm, The reagent is dispensed into the reaction container 32 transported to a predetermined position by the reaction table 3, and the reagent is transferred into the reaction container 32 on the reaction table 3 at a predetermined timing.
  • the reagent dispensing device 7 also includes a liquid level detection unit (not shown) that detects the reagent liquid level stored in the reagent container 42.
  • the dispensing probe cleaning device 6 is provided between the sample table 2 and the reaction table 3 and is provided in the middle of the horizontal movement locus of the dispensing probe 50 (see FIG. 2) in the sample dispensing device 5. In order to prevent carryover in between, the dispensing probe 50 is washed by the dispensing probe washing device 6 every time the sample is dispensed by the dispensing probe 50.
  • the dispensing probe cleaning device 8 is provided between the reagent table 4 and the reaction table 3 and in the middle of the trajectory of the horizontal movement of the dispensing probe in the reagent dispensing device 7 to prevent carryover between reagents. Therefore, every time the reagent is dispensed by the dispensing probe, the dispensing probe is cleaned by the dispensing probe cleaning device 8.
  • the control mechanism 10 includes a control unit 101, an input unit 102, an analysis unit 103, a storage unit 104, an output unit 105, a transmission / reception unit 107, and a sample dispensing control unit 108.
  • Each unit included in the control mechanism 10 is electrically connected to the control unit 101.
  • the control unit 101 is configured using a CPU or the like, and controls processing and operation of each unit of the automatic analyzer 1.
  • the control unit 101 performs predetermined input / output control on information input / output to / from each of these components, and performs predetermined information processing on this information.
  • the analysis unit 103 is connected to the photometric device 33 via the control unit 101, analyzes the component concentration of the sample based on the amount of light received by the light receiving unit 33 b, and outputs the analysis result to the control unit 101.
  • the input unit 102 is a part that performs an operation of inputting an inspection item or the like to the control unit 101. For example, a keyboard or a mouse is used.
  • the storage unit 104 is configured by using a hard disk that magnetically stores information and a memory that loads various programs related to the process from the hard disk and electrically stores them when the automatic analyzer 1 executes the process. Various information including the analysis result of the specimen is stored.
  • the storage unit 104 may include an auxiliary storage device that can read information stored in a storage medium such as a CD-ROM, a DVD-ROM, or a PC card.
  • the storage unit 104 stores information required for analysis of all analysis items that can be analyzed, for example, analysis conditions such as sample dispensing amount and sample aspiration amount, and examination items (analysis items) input by the input unit 102. ) May be stored.
  • the output unit 105 is configured using a printer, a speaker, and the like, and outputs various information related to analysis under the control of the control unit 101.
  • the output unit 105 includes a display unit 106 configured using a display or the like.
  • the display unit 106 displays analysis contents, alarms, and the like, and a display panel or the like is used.
  • the input unit 102 and the display unit 106 may be realized by a touch panel.
  • the transmission / reception unit 107 has a function as an interface for performing transmission / reception of information according to a predetermined format via a communication network (not shown).
  • the sample dispensing control unit 108 includes a determination unit 108a, a change unit 108b, an agitation control unit 108c, and a calculation unit 108d.
  • the determination unit 108a determines whether or not there is an analysis item HbA1c using the whole blood sample as a sample analysis item read from the recording medium attached to the sample container 22 by the reading unit 23.
  • the information may be recorded on the recording medium of the sample container 22 or may be stored in the storage unit 104 by the operator via the input unit 102.
  • the changing unit 108b changes the order of the analysis items of the sample to all The analysis item (HbA1c) using the blood sample as a sample is changed to the last.
  • the agitation control unit 108c lowers the dispensing probe of the sample dispensing device 5 into the sample container 22, and after the dispensing probe reaches the sample liquid level detected by the liquid level detection unit 60, the sample is sampled by the dispensing probe.
  • the sample is controlled to be agitated by repeating aspiration and discharge a plurality of times. It is preferable that the stirring of the sample by repeating the suction and discharge of the sample by the dispensing probe is started from the suction in order to prevent foaming due to air mixing into the sample. Further, it is preferable for control that the sample suction amount and the discharge amount by the dispensing probe are the same, but by setting only the first suction amount large, a dummy sample can be secured in the dispensing probe. It is possible to prevent air mixing.
  • the calculation unit 108d calculates the sample height and the sample aspiration position based on the sample container information acquired by the reading unit 23 and the sample liquid level position detected by the liquid level detection unit 60.
  • the sample dispensing device 5 dispenses a sample from the sample container 22 to the reaction container 32.
  • the reagent dispensing device 7 dispenses a reagent from the reagent container 42 into the reaction container 32.
  • the reaction container 32 into which the sample and the reagent are dispensed reacts while the sample and the reagent are agitated while being conveyed along the circumferential direction by the reaction table 3, and passes between the light source 33 a and the light receiving unit 33 b. pass.
  • the analysis light emitted from the light source 33a and passed through the reaction solution in the reaction vessel 32 is measured by the light receiving unit 33b and analyzed for component concentration and the like.
  • the reaction vessel 32 is used by the reaction vessel washing device 34 after the measurement reaction solution is discharged and washed, and then used again for analyzing the specimen.
  • FIG. 2 is a schematic diagram of the sample dispensing device 5.
  • the sample dispensing device 5 includes a dispensing probe 50, a probe driving unit 53, a dispensing pump 55, a pump driving unit 56, a liquid feeding pump 59, a tank 57, and a liquid level detecting unit 60. Yes.
  • the sample dispensing apparatus 5 is provided with a dispensing probe 50 that rotates in a horizontal plane and dispenses a sample to an arm that is moved up and down.
  • the dispensing probe 50 is made of a conductive metal material such as stainless steel.
  • the dispensing probe 50 is connected to a dispensing pump 55 by a pipe 51, and the dispensing pump 55 and the liquid feeding pump 59 are connected via a pipe 52.
  • the dispensing probe 50 is moved by the probe drive unit 53 in the horizontal direction indicated by the arrow X and the vertical direction indicated by Z in the drawing, and is transferred from the sample container 22 conveyed to the lower part of the dispensing probe 60 according to the analysis item.
  • a plasma sample or whole blood sample is aspirated, and the aspirated sample is discharged into the reaction container 32 on the reaction table 3 to dispense the sample.
  • the dispensing pump 55 is a syringe pump that sucks the sample in the sample container 22 with the dispensing probe 50 and then discharges the sample sucked into the reaction container 32 conveyed by the reaction table 3.
  • the piston 55b is reciprocated within the syringe 55a.
  • the liquid feed pump 59 sucks up the deaerated extruded water L1 stored in the tank 57 and pumps it into the pipe 52 through the electromagnetic valve 58.
  • the electromagnetic valve 58 is switched to “open” when the pumped water L1 sucked up into the pipe 52 is pumped into the pipe 52 by the control signal from the control unit 101, and the dispensing probe 55 causes the dispensing probe 50 to be Is sucked and discharged, it is switched to “closed”.
  • the liquid level detection unit 60 detects the liquid level of the sample stored in the sample container 22.
  • the liquid level detection unit 60 includes an oscillation circuit 61 and a liquid level detection circuit 62, and a metal plate 22 c is disposed near the bottom of the sample container 22. As shown in FIG. 2, the oscillation circuit 61 and the metal plate 22c are grounded.
  • the liquid level detection unit 60 detects the liquid level based on the change in capacitance between the dispensing probe 50 and the sample in the sample container 22 based on the oscillation signal oscillated by the oscillation circuit 61.
  • the liquid level detection circuit 62 includes an amplifier 62a that amplifies the oscillation signal introduced into the amplifier 62a, a diode 62b that rectifies the oscillation signal amplified by the amplifier 62a, a capacitor 62c that smoothes the rectified signal, A comparator 62d for comparing the smoothed signal with the reference signal.
  • the comparator 62d outputs a liquid level detection signal to the control unit 101 when the input smoothing signal (voltage) is larger than the reference value signal (voltage).
  • a capacitance type liquid level detection method is adopted, but a liquid level may be detected using an optical element such as a CCD.
  • FIG. 3 is a flowchart of the sample dispensing process according to the embodiment of the present invention.
  • the reading unit 23 reads the analysis item information and sample container information of the sample from the storage medium attached to the sample container 22 of the sample to be analyzed, and the sample dispensing control unit via the control unit 101 reads the information. (Step S101). Based on the transmitted analysis item information, the determination unit 108a determines whether or not HbA1c, which is an analysis item using whole blood as a sample, has been ordered for the sample (step S102). When the determination unit 108a determines that there is an analysis item (HbA1c) using a whole blood sample as a sample (Yes in step S102), the determination unit 108a further uses plasma for the sample as a sample based on the transmitted analysis item information.
  • step S103 It is determined whether or not the analysis item to be ordered has been ordered (step S103).
  • the determination unit 108a determines that there is an analysis item using a plasma sample as a sample (Yes in step S103)
  • the changing unit 108b adds HbA1c, which is an analysis item using whole blood as a sample, to the end of the analysis item of the sample.
  • the analysis order is changed so as to perform analysis (step S104).
  • the sample collected in the sample container 22 is subjected to pretreatment such as centrifugation, and then the sample table 2 of the automatic analyzer 1. Therefore, after the plasma sample, which is the upper layer component, is first dispensed, the analysis items are replaced in order to agitate the sample and dispense the whole blood sample.
  • the sample dispensing control unit 108 lowers the dispensing probe 50 into the sample container 22.
  • the liquid level detector 60 detects the sample liquid level based on the change in capacitance between the dispensing probe 50 and the sample in the sample container 22 (step S105).
  • the sample dispensing control unit 108 sucks the plasma sample with the dispensing probe 50 (step S106), transfers the dispensing probe 50 to the reaction table 3 by driving the probe driving unit 53, and then sucked plasma.
  • the specimen is discharged into the reaction container 32 (step S107).
  • the sample dispensing control unit 108 confirms whether or not dispensing of all plasma samples has been completed for the sample (step S108). When dispensing of all the plasma samples is completed for the sample (step S108, Yes), the process proceeds to step S109, while when dispensing of all the plasma samples is not completed for the sample. (Step S108, No), the process proceeds to Step S105, and a plasma sample is dispensed.
  • the liquid level is detected by the liquid level detection unit 60 (step S109), and based on the detected liquid level position and the analysis item information and sample container information acquired by the reading unit 23 in step S101, the calculation unit 108d
  • the height h (see FIG. 4 (4-2)) and the whole blood sample suction position are calculated (step S110).
  • the specimen height h is calculated from the liquid level position and specimen container information.
  • the whole blood sample aspiration position is obtained by multiplying the calculated sample height h by the collection position set by the user (for example, 20%, 30%, etc. as a ratio to the sample height).
  • the specimen separated in layers is stirred by repeating the suction and discharge of the specimen by the dispensing probe 50 under the control of the stirring control unit 108c (step S111).
  • the dispensing probe 50 performs agitation by repeating the suction and discharge of the separated sample at least twice.
  • the first suction and discharge of the specimen is preferably performed by sucking plasma near the liquid surface.
  • the sample to be aspirated for the second time and thereafter becomes a sample in which plasma and blood cells are partially mixed (has a lower viscosity than the blood cell sample), so that the dispensing probe 50 having a small inner diameter can be easily aspirated and discharged. it can.
  • the sample is aspirated and discharged by the dispensing probe 50 at different height positions of the sample, and the sample aspirating and discharging positions may be set in advance. For example, when the sample height h is set to perform stirring by suction and discharge at 0.7 h and 0.3 h, first, the dispensing probe 50 is lowered to the sample height of 0.7 h, and then the sample is aspirated and discharged. After the ejection, the dispensing probe 50 is further lowered to the specimen height of 0.3 h, and then the specimen is aspirated and ejected. More specifically, the agitation control unit 108c drives the probe driving unit 53 and the pump control unit 56 to agitate the descent of the dispensing probe 50 and the suction and discharge of the sample twice or more. I do.
  • FIG. 4 is an operation diagram of whole blood sample dispensing when the sample aspirating and discharging positions are set in advance.
  • the stirring control unit 108c lowers the dispensing probe 50 into the sample container 22 (FIG. 4 (4-1)), and after detecting the sample liquid level (FIG. 4 (4-2)), the sample calculated by the calculation unit
  • the dispensing probe 50 is lowered to a preset sample aspirating and discharging position based on the height h, and the plasma sample is aspirated and discharged (FIG. 4 (4-3)).
  • the agitation control unit 108c lowers the dispensing probe 50 to the next sample aspirating and discharging position, and agitates the sample by aspirating and discharging the sample partially mixed with plasma and blood cells (FIG. 4 (4-4)). After agitation, the agitation control unit 108c moves the dispensing probe 50 to the whole blood sample aspiration position calculated by the calculation unit 108d, aspirates the whole blood sample (FIG. 4 (4-5)), and reacts the aspirated sample. Dispense into container.
  • the position where the specimen is finally aspirated and discharged is the same as the whole blood specimen aspirating position, but it is not limited to this. It is not a thing.
  • the sample may be aspirated and discharged from the bottom surface of the sample container 22 and stirred, and then the whole blood sample may be aspirated by raising the dispensing probe 50 to the whole blood sample aspirating position.
  • the suction and discharge by the dispensing probe 50 may be performed while the dispensing probe 50 is lowered.
  • the descending speed of the dispensing probe 50, the suction time and discharge time per time, and the number of times (the suction speed and the discharge speed are constant) may be set in advance. It should be noted that in both cases where the dispensing probe 50 is lowered and aspirated and discharged alternately, and in the case where the suction and discharge are performed while the dispensing probe 50 is lowered, bubbles are caused by the introduction of air into the sample.
  • the initial aspiration amount of the sample is set larger than the discharge amount by a dummy amount, and the aspirated dummy amount is held in the dispensing probe, thereby Mixing can be reliably prevented.
  • the agitation control unit 108c once lowers the dispensing probe 50 to the bottom surface of the specimen container 22 to cause convection of the specimen due to the lowering, and then aspirates and discharges the specimen while raising the dispensing probe 50. May be performed.
  • the sample dispensing control unit 108 moves the dispensing probe to the whole blood sample aspirating position and aspirates the whole blood sample (step S112).
  • the agitation control unit 108c transfers the dispensing probe 50, which has aspirated the whole blood sample by driving the probe driving unit 53, to the reaction table 3 and discharges it to the predetermined reaction container 32 (step S113).
  • the sample dispensing control unit 108 checks whether or not dispensing for all the samples set in the sample table 2 has been completed (step S114). When the dispensing of all the samples has been completed (step S114, Yes), the sample dispensing process is terminated, and when the dispensing of all the samples has not been completed (No at step S114), the process proceeds to step S101. To do.
  • step S115 the sample dispensing control unit 108 uses the liquid level detection unit 60 to perform the sample.
  • the plasma sample is aspirated by the dispensing probe 50 (step S116), and the aspirated plasma sample is discharged into the reaction container 32 (step S117).
  • the sample dispensing control unit 108 repeats steps S115 to S117 until dispensing of all plasma samples is completed for the sample (step S118, No), and dispensing of all plasma samples is completed for the sample. If yes (step S118, Yes), the process proceeds to step S114.
  • step S109 Dispense whole blood samples. An analysis item using whole blood as a sample is ordered, but a sample for which an analysis item using plasma as a sample is not ordered is set in the sample table 2 in a whole blood state without being subjected to centrifugation. However, since the blood cell layer may settle before dispensing is started, the sample is also agitated by the agitation method in steps S109 to S111 described above, and then the whole blood sample is dispensed. .
  • the automatic analyzer 1 it is possible to collect a whole blood sample without performing the work of inversion mixing of the sample container by the user, and thereby, the cap at the time of inversion mixing of the sample container In addition to preventing sample contamination due to misplacement, etc., it is also possible to prevent dispensing failures due to bubbles that may be caused by overturning mixing. Furthermore, according to the automatic analyzer of the present embodiment, it is possible to mix new blood cells and old blood cells in the separated blood cell layer, so that more accurate measurement values can be obtained.
  • a control program for controlling processing executed by the automatic analyzer 1 is installed in the storage unit 104 of the control mechanism 10 shown in FIG.
  • the computer can function as part or all of the control mechanism 10 (FIG. 1).
  • Such a control program may be installed in the memory before the computer is shipped, or may be installed in the memory after the computer is shipped.
  • the program may be installed in the computer memory by reading the program recorded in the recording medium, or the program downloaded via a network such as the Internet may be installed in the computer memory. Any type of computer can be used as the computer.
  • control mechanism 3 (FIG. 1).
  • the operation of the control mechanism 10 means that a control method corresponding to the installed control program is executed. This is because the control method corresponds to the operation method of the control mechanism.
  • the automatic dispensing device, the sample agitation method, and the sample dispensing method of the present invention are useful for an analyzer that analyzes both a plasma sample and a whole blood sample, and particularly an analysis that requires analysis accuracy. Suitable for the field of equipment.

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
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  • General Physics & Mathematics (AREA)
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Abstract

L'invention porte sur un dispositif d'analyse automatique qui permet d'éviter un mélange à tambour vertical des contenants des substances sujets, ce qui est un fardeau pour les utilisateurs, tout en supprimant l'augmentation du coût du dispositif. L'invention porte également sur un procédé d'agitation des substances sujets et sur un procédé de distribution des substances sujets pour le dispositif d'analyse automatique. Un dispositif d'analyse automatique (1) distribue une substance sujet reçue dans un contenant de substance sujet (22) à une cuve de réaction (32) à l'aide d'un dispositif de distribution de substance sujet (5) et analyse la substance sujet en amenant la substance sujet à réagir avec un réactif à l'intérieur de la cuve de réaction (32). Le dispositif d'analyse automatique comporte un moyen de détection de surface de liquide qui détecte la surface de liquide de la substance sujet reçue dans le contenant de substance sujet (22), et une unité de commande d'agitation (108c) qui effectue une commande de façon à ce qu'une sonde de distribution du dispositif de distribution de substance sujet (5) soit abaissée vers l'intérieur de chaque contenant de substance sujet (22) et aspire et éjecte de façon répétée la substance sujet une pluralité de fois après que la sonde de distribution a atteint la surface de liquide de la substance sujet détectée par le moyen de détection de surface de liquide, de telle sorte que la substance sujet est agitée.
PCT/JP2010/007350 2009-12-18 2010-12-17 Dispositif d'analyse automatique WO2011074273A1 (fr)

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JP2012063179A (ja) * 2010-09-14 2012-03-29 Toshiba Corp 自動分析装置
JP2016166876A (ja) * 2012-12-19 2016-09-15 株式会社日立ハイテクノロジーズ 自動分析装置および分析方法
JPWO2017006969A1 (ja) * 2015-07-07 2018-04-19 コニカミノルタ株式会社 検出装置および検出方法
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CN113358885A (zh) * 2020-03-06 2021-09-07 株式会社日立高新技术 自动分析装置
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JP2017156249A (ja) * 2016-03-02 2017-09-07 アークレイ株式会社 分注装置、それを含む測定システム、および分注方法
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CN114062100A (zh) * 2022-01-11 2022-02-18 深圳市瑞图生物技术有限公司 精液混匀方法、装置、精子质量检测仪及存储介质

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