WO2011059212A2 - Preparation method of ginseng and red ginseng concentrates containing the increased amount of compound k by using pectinex - Google Patents

Preparation method of ginseng and red ginseng concentrates containing the increased amount of compound k by using pectinex Download PDF

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Publication number
WO2011059212A2
WO2011059212A2 PCT/KR2010/007862 KR2010007862W WO2011059212A2 WO 2011059212 A2 WO2011059212 A2 WO 2011059212A2 KR 2010007862 W KR2010007862 W KR 2010007862W WO 2011059212 A2 WO2011059212 A2 WO 2011059212A2
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WO
WIPO (PCT)
Prior art keywords
ginseng
compound
red
concentrates
pectinex
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Application number
PCT/KR2010/007862
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English (en)
French (fr)
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WO2011059212A3 (en
Inventor
Jin Hee Lee
Seok Jun Park
Yong Ki Seo
Yeong Eun Kim
Min Ju Park
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Cj Cheiljedang Corporation
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Application filed by Cj Cheiljedang Corporation filed Critical Cj Cheiljedang Corporation
Publication of WO2011059212A2 publication Critical patent/WO2011059212A2/en
Publication of WO2011059212A3 publication Critical patent/WO2011059212A3/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A23L2/08—Concentrating or drying of juices
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258—Panax (ginseng)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives

Definitions

  • the present invention relates to a preparation method of ginseng and red ginseng concentrates that have an amount of compound K increased by fermenting ginseng saponin among the extract of ginseng, red ginseng, or root hair of ginseng using pectinex, for use as a raw material for foods or health functional foods.
  • Ginseng is a kind of perennial herb belonging to the Panax genus in the family Araliacease, and its roots are called a ginseng radix that can be used for medicinal purposes.
  • Garriques in USA started the first scientific research related to the ingredients of ginseng. Garriques separated amorphous glycosides from American ginseng at 1854, and named it panaquilon and then reported it. Since Brekhman who was a pharmacologist in the Soviet Union at 1957 reported saponin glycoside as an active ingredient that shows the medical effectiveness of ginseng, an intensive research for saponin was started.
  • 20-0- ⁇ -D-glucophyranosyl-20(S)-protophanaxadiol (hereinafter, be called as 'Compound K') is one of PPD saponin of which the 20 th carbon of aglycon that is a basic framework of ginsenoside binds to one glucose, and is the conversion product produced by enterobacteria from ginsenoside Rb1, Rb2 and Rc that are a diol saponin of ginseng (Hasegawa, H. et al ., Planta Medica, 62:453-457(1996)).
  • the compound K is known to have the effects, such as the suppressing of cancer cell proliferation and metastasis, an anti-aging, an anti-allergy and an enhancement of immunity.
  • the compound K is not contained in ginseng itself, but may be only obtained as an internal metabolite produced by enterobacteria.
  • enterobacteria since the type and metabolic ability of an enterobacteria vary according to each person, an absorption and effectiveness can be different to each individual (Hasegawa, H. et al. , Planta Med., 63(5);436-440 (1997)).
  • the method for converting ginseng saponin is reported to include heat-treating (Kitagawa et al. , Yakugaku Zasshi., 103: 612-622(1983); Kwon et al. , J. Chromatogr. A., 921:335-339(2001); Park, Food Ind. Nutr., 9:23-27(2004)), acid-treating (Han et al. , Planta Medica., 44:146-149(1982); Bae et al. , Arch Pharm Res. 27(1):61-67(2004)), alkali-treating (Chen et al. , Chem. Pharm. Bull.
  • the method using an enzyme among those methods may be most effective to convert ginseng saponin, because it can make a selectively conversion to be possible with a specific response to a substrate without any by-products from the reaction, can make the reaction to be performed under relatively stable conditions, and can show high efficiency.
  • Korean Patent Registration No. 10-0418604 discloses a method for manufacturing the compound K at a high yield by being reacted with at least one of pectinase isolated from Aspergillus genus and naringinase isolated from Penicillium genus.
  • the organic solvents being unacceptable for foods are used in the process of isolating a purified saponin being used as a substrate from ginseng and isolating the compound K to obtain at a high yield, so that the method may not be applied for foods.
  • Korean Patent Publication No. 10-2008-0094275 discloses an example for producing the compound K by using a method for enzyme conversion with pectinex as embodiments; the yield is 0.8%, not high.
  • the present inventors use only water or alcohol that is useful for preparation of the food raw material under the Korean Food Sanitation Act in order to use as subject material of foods or healthy functional foods.
  • the ginseng or red-ginseng concentrate containing the increased contents of the compound K with the enzyme-fermentation by using pectinex that is a polysaccharide lyases could be manufactured.
  • an object of the present invention is to provide the preparation method of ginseng and red ginseng concentrates containing the increased amount of compound K with the enzyme-fermentation of ginseng saponin of the extracts of ginseng, red-ginseng, or root hair of ginseng extracted by using water or alcohol as solvent, by using pectinex.
  • the present invention provides a preparation method of ginseng and red ginseng concentrates containing the increased amount of compound K, comprising the steps of:
  • the present invention provides ginseng and red-ginseng concentrates containing the increased amount of compound K that is not contained in the existing ginseng and red-ginseng concentrate.
  • the ginseng and red-ginseng concentrates are converted into ginsenoside having the functionality through the fermentation process by using enzyme so that the ginseng and red-ginseng concentrates can be contained with the increased amount of compound K that has high intestinal digestion ability and absorption ability, thereby being utilized as a material for foods or healthy functional Foods.
  • Fig. 1 is HPLC chromatogram of standard product of compound K (99%);
  • Fig. 2 is HPLC chromatogram of red-ginseng water-extracts before enzyme-treating
  • Fig. 3 is HPLC chromatogram after reacting for 54hrs at 180 rpm by treating with 5%(v/v) pectinex, after mixing the extract of red-ginseng extracted by using water with eight times of water.
  • the present invention provides a preparation method of preparing ginseng and red ginseng concentrates containing the increased amount of compound K, comprising the steps of:
  • the solvent used in the present invention is limited to water or fermentation alcohol that is able to use as a raw material for foods.
  • the fermentation alcohol is preferably 60 to 70% alcohol, and more preferably 70% alcohol.
  • the extract is preferably concentrated to more than 65 Brix by concentrating under reduced pressure after cooling and filtering.
  • the concentrates are diluted by mixing with six to eight times of water based on the weight of the concentrate, preferably the diluted concentrates reacted with pectinex which are added with 5 to 10% of total volume.
  • the pectinex used in the present invention may be originated from Aspergillus aculeatus and Aspergillus niger , and preferably may be Pectinex AFP-L4 by Novozymes.
  • the preparation method of ginseng and red-ginseng concentrates containing the increased amount of compound K is as follows:
  • reaction temperature is not specially limited if enzyme can be activated, and is preferably 45 to 55°C, most preferably 55°C.
  • reaction time is also not specially limited if the activity of enzyme can be maintained, and is preferably 24 to 72 hrs, more preferably 48 to 60 hrs.
  • the enzyme was inactivated by heating the reaction solution in effervescent water bath for 10 minutes, so as not to occur the reaction any more.
  • the concentrates containing the increased amount of compound K were prepared by concentrating the reaction solution under reduced pressure.
  • the present invention provides foods or healthy functional foods by using the concentrates containing the increased amount of compound K.
  • ginseng extract 200 ml of 50 to 80% alcohol was added to 20 g of ginseng powder as a raw material, and extracted repeatedly 3 times for 24 hrs at 70°C, then the ginseng extract was prepared.
  • ginseng extract 200 ml of 70% alcohol was added to 20 g of ginseng powder as a raw material and extracted repeatedly 3 times for 10 hrs at 40 to 80°C, and then the ginseng extract was prepared.
  • Example 3 Preparation of Water Extracts containing the increased content of compound K using root hair of Ginseng
  • Example 4 Preparation of 70% Alcohol Extracts containing the increased content of compound K using root-hair of Ginseng
  • Example 5 Preparation of Extracts containing the increased content of compound K using Red-Ginseng

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Medical Informatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)
PCT/KR2010/007862 2009-11-12 2010-11-10 Preparation method of ginseng and red ginseng concentrates containing the increased amount of compound k by using pectinex WO2011059212A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020090109166A KR20110052218A (ko) 2009-11-12 2009-11-12 펙티넥스를 이용하여 화합물 ｋ의 함량이 증가된 인삼 및 홍삼 농축액의 제조방법
KR10-2009-0109166 2009-11-12

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WO2011059212A2 true WO2011059212A2 (en) 2011-05-19
WO2011059212A3 WO2011059212A3 (en) 2011-09-29

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102352402A (zh) * 2011-07-29 2012-02-15 金凤燮 红参皂苷Rg3组和Rh2组混合皂苷的制备方法
CN115400157A (zh) * 2021-05-26 2022-11-29 大东高丽蔘株式会社 红参水解浓缩液的特定成分增强生产工艺

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013109062A1 (ko) * 2012-01-20 2013-07-25 씨제이제일제당(주) 효소전환 및 분획기술을 이용한 사포닌 전환체 화합물 케이-강화된 분획물 및 그 제조방법
KR102466825B1 (ko) * 2020-10-05 2022-11-16 주식회사 비티씨 사포닌에 대해 생물전환능을 갖는 락토바실러스 애시도필러스 bla-9 균주, 이를 이용한 화합물 k의 함량이 증대된 발효 홍삼 추출물의 제조 방법, 이에 따른 발효 홍삼 추출물 및 이를 포함하는 건강기능식품 조성물

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020009756A (ko) * 2000-07-26 2002-02-02 박명규 효소적 방법에 의한 진세노사이드 컴파운드 케이의 제조방법
KR100418604B1 (ko) * 2001-11-01 2004-02-11 주식회사 태평양 인삼 사포닌으로부터 화합물 ｋ 및 진세노사이드 ｆ1을제조하는 방법
KR20080094275A (ko) * 2007-04-19 2008-10-23 메타볼랩(주) 진세노사이드의 효소전환

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020009756A (ko) * 2000-07-26 2002-02-02 박명규 효소적 방법에 의한 진세노사이드 컴파운드 케이의 제조방법
KR100418604B1 (ko) * 2001-11-01 2004-02-11 주식회사 태평양 인삼 사포닌으로부터 화합물 ｋ 및 진세노사이드 ｆ1을제조하는 방법
KR20080094275A (ko) * 2007-04-19 2008-10-23 메타볼랩(주) 진세노사이드의 효소전환

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102352402A (zh) * 2011-07-29 2012-02-15 金凤燮 红参皂苷Rg3组和Rh2组混合皂苷的制备方法
CN115400157A (zh) * 2021-05-26 2022-11-29 大东高丽蔘株式会社 红参水解浓缩液的特定成分增强生产工艺
EP4094770A1 (en) * 2021-05-26 2022-11-30 Daedong Korea Ginseng Co. Ltd. Process for production of red ginseng hydrolysis concentrate having enriched specific component
US11759490B2 (en) 2021-05-26 2023-09-19 Daedong Korea Ginseng Co., Ltd Process for production of red ginseng hydrolysis concentrate having enriched specific component
CN115400157B (zh) * 2021-05-26 2024-01-30 大东高丽蔘株式会社 红参水解浓缩液的特定成分增强生产工艺

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KR20110052218A (ko) 2011-05-18

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