WO2009072593A1 - 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 - Google Patents

乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 Download PDF

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Publication number
WO2009072593A1
WO2009072593A1 PCT/JP2008/072129 JP2008072129W WO2009072593A1 WO 2009072593 A1 WO2009072593 A1 WO 2009072593A1 JP 2008072129 W JP2008072129 W JP 2008072129W WO 2009072593 A1 WO2009072593 A1 WO 2009072593A1
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WIPO (PCT)
Prior art keywords
lactic acid
expression cassette
lactate dehydrogenase
yeast
producing lactic
Prior art date
Application number
PCT/JP2008/072129
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English (en)
French (fr)
Inventor
Kenji Sawai
Hideki Sawai
Original Assignee
Toray Industries, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Toray Industries, Inc. filed Critical Toray Industries, Inc.
Priority to AU2008332348A priority Critical patent/AU2008332348B2/en
Priority to CN200880126352.4A priority patent/CN101939426B/zh
Priority to CA2707687A priority patent/CA2707687A1/en
Priority to JP2009504930A priority patent/JP5532919B2/ja
Priority to ES08856191.5T priority patent/ES2616428T3/es
Priority to EP08856191.5A priority patent/EP2221373B1/en
Priority to US12/746,642 priority patent/US8859260B2/en
Priority to BRPI0818979-0A2A priority patent/BRPI0818979A2/pt
Publication of WO2009072593A1 publication Critical patent/WO2009072593A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　高い光学純度、乳酸収率ならびに乳酸生産速度を同時に達成することが可能となる、乳酸生産能力を有する酵母を分離膜で濾過しながら行う連続培養において、乳酸収率ならびに乳酸生産速度の低下を防ぐために必要となる乳酸脱水素酵素をコードする遺伝子発現カセット、該カセットを有する酵母ならびに該酵母を培養することによる乳酸の製造方法を提供する。 　本発明の乳酸脱水素酵素発現カセットは、プロモーターの下流に乳酸脱水素酵素をコードする遺伝子が連結された乳酸脱水素酵素発現カセットであって、乳酸生産能力を有する酵母を分離膜で濾過しながら行う連続培養において、前記プロモーターは、培養開始後５０時間以降における遺伝子発現量が全遺伝子の平均相対発現量の５倍以上である遺伝子のプロモーターである、乳酸脱水素酵素発現カセットである。
PCT/JP2008/072129 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法 WO2009072593A1 (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
AU2008332348A AU2008332348B2 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
CN200880126352.4A CN101939426B (zh) 2007-12-07 2008-12-05 乳酸脱氢酶表达盒、转化酵母和乳酸的制造方法
CA2707687A CA2707687A1 (en) 2007-12-07 2008-12-05 Lactate dehydrogenase-expressing cassette, transformant yeast strain and method of producing lactic acid
JP2009504930A JP5532919B2 (ja) 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法
ES08856191.5T ES2616428T3 (es) 2007-12-07 2008-12-05 Casete de expresión para lactasa deshidrogenasa, levadura transformada y método para producir ácido láctico
EP08856191.5A EP2221373B1 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
US12/746,642 US8859260B2 (en) 2007-12-07 2008-12-05 Expression cassette for lactase dehydrogenase, transformed yeast and method of producing lactic acid
BRPI0818979-0A2A BRPI0818979A2 (pt) 2007-12-07 2008-12-05 "cassete de expressão de lactato desidrogenase, cepa de levedura transformante e método de produzir ácido láctico"

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-317566 2007-12-07
JP2007317566 2007-12-07

Publications (1)

Publication Number Publication Date
WO2009072593A1 true WO2009072593A1 (ja) 2009-06-11

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/072129 WO2009072593A1 (ja) 2007-12-07 2008-12-05 乳酸脱水素酵素発現カセット、形質転換酵母および乳酸の製造方法

Country Status (10)

Country Link
US (1) US8859260B2 (ja)
EP (1) EP2221373B1 (ja)
JP (1) JP5532919B2 (ja)
KR (1) KR20100094525A (ja)
CN (1) CN101939426B (ja)
AU (1) AU2008332348B2 (ja)
BR (1) BRPI0818979A2 (ja)
CA (1) CA2707687A1 (ja)
ES (1) ES2616428T3 (ja)
WO (1) WO2009072593A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8822195B2 (en) 2009-06-03 2014-09-02 Toray Industries, Inc. Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid
JP2017023953A (ja) * 2015-07-23 2017-02-02 株式会社東芝 処理装置及び処理方法
CN113699090A (zh) * 2021-09-09 2021-11-26 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101939439B (zh) * 2008-02-04 2014-08-06 东丽株式会社 通过连续发酵实施的乳酸的制造方法
KR20130001103A (ko) * 2011-06-24 2013-01-03 삼성전자주식회사 젖산의 고효율 생산을 위한 변형 미생물
US9150835B2 (en) * 2011-06-24 2015-10-06 Samsung Electronics Co., Ltd. Modified microorganism for highly efficient production of lactic acid
FR2988733B1 (fr) * 2012-03-27 2016-02-05 Carbios Microorganisme recombinant
US9593350B2 (en) 2014-02-14 2017-03-14 Samsung Electronics Co., Ltd. Lactate dehydrogenase mutant, polynucleotide coding for the mutant, yeast cell including the polynucleotide, method of preparing the mutant, and method of producing the lactate using the same
PT3143075T (pt) 2014-05-16 2022-08-12 Carbios Processo de reciclagem de artigos de plástico pet mistos
DK3209771T3 (da) 2014-10-21 2021-01-11 Carbios Polypeptid med en polyester-nedbrydende aktivitet og anvendelser deraf
EP3233995B1 (en) 2014-12-19 2023-08-09 Carbios Plastic compound and preparation process
US10508269B2 (en) 2015-03-13 2019-12-17 Carbios Polypeptide having a polyester degrading activity and uses thereof
JP6907132B2 (ja) 2015-06-12 2021-07-21 キャルビオスＣａｒｂｉｏｓ 生分解性ポリエステル組成物及びその使用
WO2017108577A1 (en) 2015-12-21 2017-06-29 Carbios Recombinant yeast cells producing polylactic acid and uses thereof
WO2017198786A1 (en) 2016-05-19 2017-11-23 Carbios A process for degrading plastic products
CN109943492B (zh) * 2019-04-03 2022-08-16 广东省微生物研究所（广东省微生物分析检测中心） 一种重组酵母菌株及其应用

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516584A (ja) 1997-09-12 2001-10-02 エイ・イー・スタリー・マニユフアクチヤリング・カンパニー 乳酸生産のための酵母菌株
WO2002064240A1 (fr) 2001-02-16 2002-08-22 Toray Industries, Inc. Film de separation, element de film de separation, module de film de separation, dispositif de traitement d'eaux usees et residuaires, et procede de fabrication de film de separation
JP2003093060A (ja) 2001-09-20 2003-04-02 Toyota Motor Corp 耐酸性微生物を用いた有機酸及びアルコールの製造方法
JP2003265177A (ja) * 2002-03-13 2003-09-24 Gekkeikan Sake Co Ltd タンパク質の高発現システム
JP2005137306A (ja) 2003-11-07 2005-06-02 Toyota Central Res & Dev Lab Inc 有機酸存在下におけるプロモーター及びその利用
JP2005245335A (ja) * 2004-03-04 2005-09-15 Gekkeikan Sake Co Ltd 目的タンパク質提示酵母及びその利用
JP2006288318A (ja) * 2005-04-13 2006-10-26 Toyota Central Res & Dev Lab Inc 酸性条件下で使用するためのプロモーター及びその利用
JP2006296377A (ja) * 2005-04-25 2006-11-02 Toyota Central Res & Dev Lab Inc 有機酸生産用形質転換体
JP2007020538A (ja) * 2005-07-21 2007-02-01 Gekkeikan Sake Co Ltd タンパク質の高発現システム
JP2007082490A (ja) * 2005-09-22 2007-04-05 Toyota Central Res & Dev Lab Inc 有機酸の抽出発酵による生産方法及び該生産方法に用いる有機酸生産酵母
WO2007043253A1 (ja) 2005-10-14 2007-04-19 Toray Industries, Inc. 酵母及びｌ-乳酸の製造方法
WO2007097260A1 (ja) 2006-02-24 2007-08-30 Toray Industries, Inc. 化学品の製造方法、および、連続発酵装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1955299A (zh) 2000-11-22 2007-05-02 内特尔沃克公司 合成有机产物的方法和材料
WO2003049525A2 (en) * 2001-11-23 2003-06-19 Cargill Dow Llc Methods and materials for the production of organic products in cells of $i(candida) species
BR0311452A (pt) 2002-05-30 2005-03-29 Cargill Dow Llc Processos e materiais para a produção de ácido lático em levedura
CN101654666B (zh) * 2003-09-30 2012-11-14 三井化学株式会社 用于生产d-乳酸的生物催化剂
US8822195B2 (en) * 2009-06-03 2014-09-02 Toray Industries, Inc. Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001516584A (ja) 1997-09-12 2001-10-02 エイ・イー・スタリー・マニユフアクチヤリング・カンパニー 乳酸生産のための酵母菌株
WO2002064240A1 (fr) 2001-02-16 2002-08-22 Toray Industries, Inc. Film de separation, element de film de separation, module de film de separation, dispositif de traitement d'eaux usees et residuaires, et procede de fabrication de film de separation
JP2003093060A (ja) 2001-09-20 2003-04-02 Toyota Motor Corp 耐酸性微生物を用いた有機酸及びアルコールの製造方法
JP2003265177A (ja) * 2002-03-13 2003-09-24 Gekkeikan Sake Co Ltd タンパク質の高発現システム
JP2005137306A (ja) 2003-11-07 2005-06-02 Toyota Central Res & Dev Lab Inc 有機酸存在下におけるプロモーター及びその利用
JP2005245335A (ja) * 2004-03-04 2005-09-15 Gekkeikan Sake Co Ltd 目的タンパク質提示酵母及びその利用
JP2006288318A (ja) * 2005-04-13 2006-10-26 Toyota Central Res & Dev Lab Inc 酸性条件下で使用するためのプロモーター及びその利用
JP2006296377A (ja) * 2005-04-25 2006-11-02 Toyota Central Res & Dev Lab Inc 有機酸生産用形質転換体
JP2007020538A (ja) * 2005-07-21 2007-02-01 Gekkeikan Sake Co Ltd タンパク質の高発現システム
JP2007082490A (ja) * 2005-09-22 2007-04-05 Toyota Central Res & Dev Lab Inc 有機酸の抽出発酵による生産方法及び該生産方法に用いる有機酸生産酵母
WO2007043253A1 (ja) 2005-10-14 2007-04-19 Toray Industries, Inc. 酵母及びｌ-乳酸の製造方法
WO2007097260A1 (ja) 2006-02-24 2007-08-30 Toray Industries, Inc. 化学品の製造方法、および、連続発酵装置

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ISHIDA N ET AL.: "D-lactic acid production by metabolically engineered Saccharomyces cerevisiae", J. BIOSCI. BIOENG., vol. 101, no. 2, 2006, pages 172 - 177, XP025182892 *
ISHIDA, TAKAHASHI ET AL., J. BIOSCI. BIOENG., vol. 101, no. 2, 2006, pages 172 - 177
KAWAHATA M ET AL.: "Yeast genes involved in response to lactic acid and acetic acid: acidic conditions caused by the organic acids in Saccharomyces cerevisiae cultures induce expression of intracellular metal metabolism genes regulated by Aftlp", FEMS YEAST RES., vol. 6, no. 6, 2006, pages 924 - 936, XP008128587 *
RES. MICROBIOL, vol. 146, 1995, pages 291 - 302
See also references of EP2221373A4 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8822195B2 (en) 2009-06-03 2014-09-02 Toray Industries, Inc. Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid
JP2017023953A (ja) * 2015-07-23 2017-02-02 株式会社東芝 処理装置及び処理方法
CN113699090A (zh) * 2021-09-09 2021-11-26 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法
CN113699090B (zh) * 2021-09-09 2023-06-20 浙江华睿生物技术有限公司 一种构建丙酸生产菌的方法

Also Published As

Publication number Publication date
JPWO2009072593A1 (ja) 2011-04-28
JP5532919B2 (ja) 2014-06-25
US8859260B2 (en) 2014-10-14
EP2221373B1 (en) 2017-02-15
EP2221373A4 (en) 2011-07-06
EP2221373A1 (en) 2010-08-25
CN101939426A (zh) 2011-01-05
AU2008332348A1 (en) 2009-06-11
CN101939426B (zh) 2014-01-08
ES2616428T3 (es) 2017-06-13
US20100273225A1 (en) 2010-10-28
BRPI0818979A2 (pt) 2014-10-14
AU2008332348B2 (en) 2014-06-26
KR20100094525A (ko) 2010-08-26
CA2707687A1 (en) 2009-06-11

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