WO2009038194A1 - 組換え微生物及びポリ－ガンマ－グルタミン酸の製造方法 - Google Patents

組換え微生物及びポリ－ガンマ－グルタミン酸の製造方法 Download PDF

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Publication number
WO2009038194A1
WO2009038194A1 PCT/JP2008/067023 JP2008067023W WO2009038194A1 WO 2009038194 A1 WO2009038194 A1 WO 2009038194A1 JP 2008067023 W JP2008067023 W JP 2008067023W WO 2009038194 A1 WO2009038194 A1 WO 2009038194A1
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gene
gamma
glutamic acid
recombinant microorganism
poly
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PCT/JP2008/067023
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English (en)
French (fr)
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Kazuhisa Sawada
Hiroshi Hagihara
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Kao Corporation
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Priority to CN200880107496.5A priority Critical patent/CN101802169B/zh
Priority to EP08831819.1A priority patent/EP2196534B1/en
Priority to US12/678,798 priority patent/US20100248308A1/en
Publication of WO2009038194A1 publication Critical patent/WO2009038194A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　ポリ－ガンマ－グルタミン酸合成に関与する遺伝子群のなかで、枯草菌における<>pgsB</>遺伝子又は当該遺伝子に相当する遺伝子と、枯草菌における<>pgsC</>遺伝子又は当該遺伝子に相当する遺伝子を宿主微生物に導入し、枯草菌における<>pgsA</>遺伝子又は当該遺伝子に相当する遺伝子を上記宿主微生物に導入していない、ポリ－ガンマ－グルタミン酸生産能を有する組換え微生物、および上記組換え微生物を用いたポリ－ガンマ－グルタミン酸の製造方法。
PCT/JP2008/067023 2007-09-20 2008-09-19 組換え微生物及びポリ－ガンマ－グルタミン酸の製造方法 WO2009038194A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN200880107496.5A CN101802169B (zh) 2007-09-20 2008-09-19 重组微生物以及聚-γ-谷氨酸的制造方法
EP08831819.1A EP2196534B1 (en) 2007-09-20 2008-09-19 A recombinant microorganism and method for producing poly-gamma-glutamic acid
US12/678,798 US20100248308A1 (en) 2007-09-20 2008-09-19 Recombinant Microorganism and a Method for Producing Poly-Gamma-Glutamic Acid

Applications Claiming Priority (2)

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JP2007-244023 2007-09-20
JP2007244023 2007-09-20

Publications (1)

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WO2009038194A1 true WO2009038194A1 (ja) 2009-03-26

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US (1) US20100248308A1 (ja)
EP (1) EP2196534B1 (ja)
JP (1) JP5551349B2 (ja)
CN (1) CN101802169B (ja)
WO (1) WO2009038194A1 (ja)

Cited By (3)

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CN103820430A (zh) * 2013-12-19 2014-05-28 河南科技大学 高gc含量或以复杂结构dna为模板的dna片段的重叠延伸pcr法
CN114426976A (zh) * 2021-12-31 2022-05-03 华东理工大学 一种耦合ATP再生酶和聚谷氨酸合成酶的γ-PGA制备方法
CN114752522A (zh) * 2022-04-11 2022-07-15 中国科学院天津工业生物技术研究所 一株高产γ-聚谷氨酸的耐盐芽孢杆菌及其发酵条件优化

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EP2172539A1 (en) * 2006-05-23 2010-04-07 Toyo Boseki Kabushiki Kaisha Microorganism capable of producing gamma-L-PGA, method for production of Gamma-L-PGA using the microorganism, crosslinked product, and agent for external application to the skin
JP5190829B2 (ja) * 2011-01-13 2013-04-24 株式会社ノースペット ペットフード及びその製造方法
JP5945168B2 (ja) * 2012-06-08 2016-07-05 花王株式会社 組換え微生物及びそれを用いたポリ−γ−グルタミン酸の製造方法
JP6088283B2 (ja) * 2013-02-19 2017-03-01 花王株式会社 ポリ−ガンマ−グルタミン酸の生産方法
WO2019142773A1 (ja) 2018-01-16 2019-07-25 花王株式会社 Ｍ２３ａファミリープロテアーゼの製造方法
CN110066758A (zh) * 2019-06-06 2019-07-30 武汉骏安生物科技有限公司 一种高产聚-γ-谷氨酸的芽胞杆菌及其构建方法和应用
US20230111102A1 (en) 2020-01-27 2023-04-13 Kao Corporation Method for producing the variable domain of the heavy chain of a heavy-chain antibody
CN113801856B (zh) * 2020-06-12 2023-12-22 华东理工大学 利用表达聚谷氨酸合成酶的重组菌静息细胞和/或聚谷氨酸合成酶制备γ-聚谷氨酸的方法
US20240060110A1 (en) 2020-10-07 2024-02-22 Basf Se Bacillus cell with reduced lipase and/or esterase side activities
JP2022071423A (ja) 2020-10-28 2022-05-16 花王株式会社 改変シグナルペプチド
CN113234764A (zh) * 2021-05-19 2021-08-10 江南大学 一种γ-聚谷氨酸的异源表达方法
US20240318188A1 (en) 2021-06-24 2024-09-26 Basf Se Bacillus licheniformis host cell for production of a compound of interest with increased purity
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
CN114369561B (zh) * 2022-01-11 2024-07-02 江南大学 调控CapBCA单体表达水平的菌株及在聚谷氨酸生产的应用
CN114874965B (zh) * 2022-06-14 2023-04-14 安徽工程大学 一种枯草芽孢杆菌工程菌及其构建方法和应用

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103820430A (zh) * 2013-12-19 2014-05-28 河南科技大学 高gc含量或以复杂结构dna为模板的dna片段的重叠延伸pcr法
CN114426976A (zh) * 2021-12-31 2022-05-03 华东理工大学 一种耦合ATP再生酶和聚谷氨酸合成酶的γ-PGA制备方法
WO2023124062A1 (zh) * 2021-12-31 2023-07-06 华东理工大学 一种耦合ATP再生酶和聚谷氨酸合成酶的γ-PGA制备方法
CN114752522A (zh) * 2022-04-11 2022-07-15 中国科学院天津工业生物技术研究所 一株高产γ-聚谷氨酸的耐盐芽孢杆菌及其发酵条件优化
CN114752522B (zh) * 2022-04-11 2023-08-04 中国科学院天津工业生物技术研究所 一株高产γ-聚谷氨酸的耐盐芽孢杆菌及其发酵条件优化

Also Published As

Publication number Publication date
CN101802169B (zh) 2015-11-25
JP5551349B2 (ja) 2014-07-16
EP2196534A4 (en) 2011-04-06
JP2009089708A (ja) 2009-04-30
CN101802169A (zh) 2010-08-11
EP2196534A1 (en) 2010-06-16
EP2196534B1 (en) 2016-08-24
US20100248308A1 (en) 2010-09-30

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