WO2008153056A1 - 細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法 - Google Patents

細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法 Download PDF

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Publication number
WO2008153056A1
WO2008153056A1 PCT/JP2008/060676 JP2008060676W WO2008153056A1 WO 2008153056 A1 WO2008153056 A1 WO 2008153056A1 JP 2008060676 W JP2008060676 W JP 2008060676W WO 2008153056 A1 WO2008153056 A1 WO 2008153056A1
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WO
WIPO (PCT)
Prior art keywords
living cells
cell
fractionating
flow cytometer
cells
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Application number
PCT/JP2008/060676
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English (en)
French (fr)
Inventor
Shigeyuki Nakada
Noriaki Kimura
Akihide Ito
Kenji Yasuda
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Mitsui Engineering & Shipbuilding Co., Ltd.
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Publication date
Application filed by Mitsui Engineering & Shipbuilding Co., Ltd. filed Critical Mitsui Engineering & Shipbuilding Co., Ltd.
Priority to JP2008546010A priority Critical patent/JP4295816B2/ja
Priority to US12/663,373 priority patent/US8642306B2/en
Priority to EP08765455A priority patent/EP2172540A1/en
Priority to CN2008800062693A priority patent/CN101622339B/zh
Publication of WO2008153056A1 publication Critical patent/WO2008153056A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
    • G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/42—Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　生細胞群を含むサンプル液流に測定用レーザ光を照射し、測定用レーザ光の照射を受けて、サンプル液流の各生細胞それぞれから発する、散乱光および蛍光の少なくともいずれか一方の光情報それぞれを取得し、取得された光情報に基づき、各光情報に対応する生細胞が、不要生細胞または目的生細胞のいずれであるかをそれぞれ判別し、判別結果に応じて、サンプル液中の生細胞群のうち不要生細胞と判別された生細胞に対してのみパルス電圧を印加し、不要生細胞に損傷を与えて死細胞とする。
PCT/JP2008/060676 2007-06-14 2008-06-11 細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法 WO2008153056A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2008546010A JP4295816B2 (ja) 2007-06-14 2008-06-11 細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法
US12/663,373 US8642306B2 (en) 2007-06-14 2008-06-11 Flow cytometer having cell-sorting function and method of separating living cells
EP08765455A EP2172540A1 (en) 2007-06-14 2008-06-11 Flow cytometer having cell-fractionation function and method of fractionating living cells
CN2008800062693A CN101622339B (zh) 2007-06-14 2008-06-11 具有细胞分级分离处理功能的流式细胞仪以及活细胞分级分离处理方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-157275 2007-06-14
JP2007157275 2007-06-14

Publications (1)

Publication Number Publication Date
WO2008153056A1 true WO2008153056A1 (ja) 2008-12-18

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Application Number Title Priority Date Filing Date
PCT/JP2008/060676 WO2008153056A1 (ja) 2007-06-14 2008-06-11 細胞分別処理機能を有するフローサイトメータ、および生細胞分別処理方法

Country Status (6)

Country Link
US (1) US8642306B2 (ja)
EP (1) EP2172540A1 (ja)
JP (1) JP4295816B2 (ja)
KR (1) KR101113167B1 (ja)
CN (1) CN101622339B (ja)
WO (1) WO2008153056A1 (ja)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011104770A1 (en) * 2010-02-26 2011-09-01 Sharp Kabushiki Kaisha Detection apparatus and method for detecting airborne biological particles
CN102381441A (zh) * 2011-06-30 2012-03-21 上海泽泉科技有限公司 用于饮用水水源地藻类监测预警的浮标
JP2012523849A (ja) * 2009-04-20 2012-10-11 オリジンオイル，インコーポレイテッド 藻類から細胞内生成物および細胞塊および砕片を得るためのシステム、装置および方法、ならびにその誘導物および使用法
WO2013069452A1 (ja) * 2011-11-08 2013-05-16 浜松ホトニクス株式会社 幹細胞の観察方法、分化傾向状態の細胞領域の除去方法、及び、幹細胞の観察装置
WO2015111293A1 (ja) * 2014-01-24 2015-07-30 ソニー株式会社 粒子分取装置及び粒子分取方法
WO2017191824A1 (ja) * 2016-05-06 2017-11-09 アライドフロー株式会社 生物学的粒子を含む液体フローを形成する装置および処理装置

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* Cited by examiner, † Cited by third party
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JP2011237201A (ja) * 2010-05-06 2011-11-24 Sony Corp 微小粒子分取装置、マイクロチップ及びマイクロチップモジュール
CN102243150B (zh) * 2010-05-14 2012-12-26 河北省农林科学院昌黎果树研究所 一种适于流式细胞分析的果树成熟叶片细胞核提取方法
EP2894459A4 (en) * 2012-09-06 2016-04-27 Furukawa Electric Co Ltd APPARATUS FOR IDENTIFICATION AND OUTPUT OF SAMPLES AND METHOD FOR IDENTIFYING AND SUBMITTING SAMPLES
CN103352068A (zh) * 2012-11-27 2013-10-16 江南大学 一种采用流式细胞术检测发酵过程中细胞活性的方法
US8773562B1 (en) 2013-01-31 2014-07-08 Apple Inc. Vertically stacked image sensor
KR102434111B1 (ko) * 2015-03-27 2022-08-19 도쿄엘렉트론가부시키가이샤 미립자 계측 장치
CN105547970B (zh) * 2015-12-22 2018-09-18 中国科学院苏州生物医学工程技术研究所 一种流式细胞仪激发光源系统及校正方法
JP6805560B2 (ja) * 2016-06-10 2020-12-23 ソニー株式会社 接続部材及び微小粒子測定装置
CN112136034A (zh) 2018-04-27 2020-12-25 贝克顿·迪金森公司 用于流式细胞术分选样品的收集系统及其使用方法
CN115895864A (zh) * 2022-11-30 2023-04-04 重庆大学 一种基于平面电极的微流控芯片检测系统

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Patent Citations (2)

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Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012523849A (ja) * 2009-04-20 2012-10-11 オリジンオイル，インコーポレイテッド 藻類から細胞内生成物および細胞塊および砕片を得るためのシステム、装置および方法、ならびにその誘導物および使用法
JP2013143957A (ja) * 2010-02-26 2013-07-25 Sharp Corp 空気中の生物由来の粒子を検出するための検出装置および検出方法
WO2011104770A1 (en) * 2010-02-26 2011-09-01 Sharp Kabushiki Kaisha Detection apparatus and method for detecting airborne biological particles
JP2013520639A (ja) * 2010-02-26 2013-06-06 シャープ株式会社 空気中の生物由来の粒子を検出するための検出装置および検出方法
CN102381441A (zh) * 2011-06-30 2012-03-21 上海泽泉科技有限公司 用于饮用水水源地藻类监测预警的浮标
JPWO2013069452A1 (ja) * 2011-11-08 2015-04-02 浜松ホトニクス株式会社 幹細胞の観察方法、分化傾向状態の細胞領域の除去方法、及び、幹細胞の観察装置
WO2013069452A1 (ja) * 2011-11-08 2013-05-16 浜松ホトニクス株式会社 幹細胞の観察方法、分化傾向状態の細胞領域の除去方法、及び、幹細胞の観察装置
US9134296B2 (en) 2011-11-08 2015-09-15 Hamamatsu Photonics K.K. Method for observing stem cells, method for removal of cell region in state tending toward differentiation, and device for observing stem cells
US9435734B2 (en) 2011-11-08 2016-09-06 Hamamatsu Photonics K.K. Method for observing stem cells, method for removal of cell region in state tending toward differentiation, and device for observing stem cells
JP2016168052A (ja) * 2011-11-08 2016-09-23 浜松ホトニクス株式会社 幹細胞の観察方法、及び幹細胞の観察装置
WO2015111293A1 (ja) * 2014-01-24 2015-07-30 ソニー株式会社 粒子分取装置及び粒子分取方法
WO2017191824A1 (ja) * 2016-05-06 2017-11-09 アライドフロー株式会社 生物学的粒子を含む液体フローを形成する装置および処理装置
JP2017201278A (ja) * 2016-05-06 2017-11-09 アライドフロー株式会社 生物学的粒子を含む液体フローを形成する装置および処理装置
US10730052B2 (en) 2016-05-06 2020-08-04 Allied Flow, Inc. Apparatus for forming liquid flow including biological particles, and treatment apparatus
US11192113B2 (en) 2016-05-06 2021-12-07 Allied Flow Inc. Treatment apparatus and sterilization treatment method for treatment apparatus

Also Published As

Publication number Publication date
US20100178682A1 (en) 2010-07-15
JP4295816B2 (ja) 2009-07-15
EP2172540A1 (en) 2010-04-07
KR20090096744A (ko) 2009-09-14
KR101113167B1 (ko) 2012-03-07
CN101622339A (zh) 2010-01-06
JPWO2008153056A1 (ja) 2010-08-26
CN101622339B (zh) 2012-05-23
US8642306B2 (en) 2014-02-04

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