WO2006068250A1 - ナノ黒鉛構造体-金属ナノ粒子複合体 - Google Patents
ナノ黒鉛構造体-金属ナノ粒子複合体 Download PDFInfo
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- WO2006068250A1 WO2006068250A1 PCT/JP2005/023675 JP2005023675W WO2006068250A1 WO 2006068250 A1 WO2006068250 A1 WO 2006068250A1 JP 2005023675 W JP2005023675 W JP 2005023675W WO 2006068250 A1 WO2006068250 A1 WO 2006068250A1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
- B82B3/00—Manufacture or treatment of nanostructures by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
- B82B3/0009—Forming specific nanostructures
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/158—Carbon nanotubes
- C01B32/168—After-treatment
- C01B32/174—Derivatisation; Solubilisation; Dispersion in solvents
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/18—Nanoonions; Nanoscrolls; Nanohorns; Nanocones; Nanowalls
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y40/00—Manufacture or treatment of nanostructures
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B2202/00—Structure or properties of carbon nanotubes
- C01B2202/02—Single-walled nanotubes
Definitions
- the present invention relates to a protein in which a nanographite structure recognition peptide is fused or chemically bound to the surface of a cage protein such as ferritin, or to a nanographite structure produced using the protein.
- the present invention relates to a nanographite structure-metal nanoparticle composite in which a plurality of nanoparticles of an inorganic metal compound are supported.
- a graphite structure compound having a nanometer-scale fine structure and a nanographite structure-metal nanoparticle composite in which multiple nanoparticles are supported via a cage protein such as fused ferritin that specifically recognizes this Can be advantageously used in semiconductors and nanobiotechnology.
- Non-Patent Document 1 C60 has a soccerball-like structure consisting of 12 pentagons and 20 hexagons, and there are large cage molecules such as C70 and C76 in the force of C60. It is called “fullerene”.
- Sumio Iijima called “Carbon Nanotubes” (Non-patent Document 2, Patent Document 1), and in 1999, also called “Carbon Nanohorn” (Non-patent Document 3, Patent Document 1).
- Carbon-based compounds with new structures that were not known until now were discovered one after another. These fullerenes, carbon nanotubes, and carbon nanohorns are all composed of 6-membered and 5-membered rings of carbon atoms, and form nanometer-scale microstructures. It attracts attention.
- Nanographite structures are attracting attention are as follows: “Carbon nanotubing force can have both metal and semiconductor properties due to its chirality” (Non-patent Document 4 ), “Metal-introduced fullerene exhibits superconductivity” (Non-patent document 5), “Selective gas storage capacity of carbon nanohorn” (Non-patent document 6), “Carbon nanohorn possessed pharmaceutical compound, sustained release capability (Patent Document 2, Non-Patent Document 7). These Utilizing the characteristic properties of new electronic materials, catalysts, optical materials, and other fields, more specifically, semiconductor wiring, fluorescent display tubes, fuel cells, gas storage, gene therapy vectors, cosmetics, pharmaceuticals Applications of nanographite structures to delivery systems and biosensors are expected.
- Kiyotaka Shiba et al. One of the inventors, has isolated a peptide motif that binds to carbon nanohorn, which is one of the nano black structures, by the phage display method (Patent Document 3, Non-Patent Document 8).
- ferritin protein has long been known as a protein that stores “iron” molecules that have both the essential metal force S and toxicity at the same time in vivo.
- Ferritin exists universally from animals and plants to bacteria, and is deeply involved in homeostasis of iron elements in living organisms and cells.
- Higher eukaryotic ferritin such as human tuma forms a spherical nuclear structure consisting of a 24-mer peptide chain with a molecular weight of about 20 kDa and a diameter of about 12 nm, and has a space of 7-8 nm inside. In this internal space, iron molecules are stored as a cluster of nanoparticulate iron oxide.
- the 24 subunits that make up the protein spherical shell (cage) are of two types (H type and L type), and the composition ratios differ depending on the species and tissue.
- Patent Document 1 JP-A-2001-64004
- Patent Document 2 # 112004-139247
- Patent Document 3 JP 2004-121154
- Non-Patent Document 1 Nature, 318: 162-163, 1985
- Non-Patent Document 2 Nature, 354: 56-58, 1991
- Non-Patent Document 3 Chem. Phys. Lett., 309: 165-170, 1999
- Non-Patent Document 4 Nature, 391: 59-62
- Non-Patent Document 5 Nature, 350: 600-601
- Non-Patent Document 6 Nikkei Science, 42, August, 2002
- Non-Patent Document 7 Mol Pharmaceutics 1: 399
- Non-Patent Document 8 Langmuir, 20, 8939-8941, 2004
- the object of the present invention is to fuse the ability of ferritin molecules having the ability to form nanoparticles of inorganic metal atoms or inorganic metal compounds to nanographite structure recognition peptides, so that carbon nanotubes' carbon nanohorns or their modifications can be efficiently produced. It is to recognize well and make it possible to carry functional compounds.
- ferritin molecules have the ability to form two-dimensional crystals at the interface and have the ability to align molecules, it is possible to align carbon nanotubes' carbon nanohorns using the molecular alignment ability of ferritin fused with nanographite structure recognition peptides. Is to make it. Means for solving the problem
- the present inventors have intensively studied to solve the above problems, and a peptide consisting of the amino acid sequence represented by SEQ ID NO: 1 is added to cDNA encoding the amino terminal of the L-type ferritin molecule derived from horse spleen.
- the encoded DNA is fused, E. coli is used to express a protein having the amino acid sequence shown in SEQ ID NO: 26, the protein is purified, and metal oxide nanoparticles are placed in the internal space of the resulting fusion protein. It was confirmed that a plurality of nanoparticles can be supported on the nanographite structure, and the present invention has been completed.
- the present invention (1) holds inorganic metal atoms or inorganic metal compound nanoparticles in the internal space of a protein in which a nanographite structure recognition peptide is fused or chemically bound to the surface of a cage protein.
- Nanographite structure-recognizing peptide using a nanographite structure with a plurality of inorganic metal atoms or inorganic metal compound nanoparticles supported on the nanographite structure Complex or (2) cage tamper The nano-black lead structure-metal nanoparticle complex described in (1) above, wherein the protein is a ferritin protein family, and (3) the ferritin protein family is ferritin (2) Nanographite structure metal nanoparticle composite according to (2)
- the ferritin derived from higher eukaryotes is a ferritin derived from a higher eukaryote, the nanographite structure-metal-nanoparticle complex described in (3) above, and (5) ferritin derived from a higher eukaryote.
- the spleen-derived L-type ferritin is characterized in that the nanographite structure-metal-metal nanoparticle complex described in (4) and (6) the cage protein are derived from bacteria (1) ) Nanographite structure / metal nanoparticle complex according to (7), or (7) the nanographite structure / metal nanoparticle complex according to (1) above, wherein the cage protein is a virus particle.
- the nanographite structure-recognizing peptide is a peptide having an amino acid sequence represented by any one of SEQ ID NOs:! To 20, as described in any one of (1) to (7) above Nanographite structure / metal nanoparticle composite, (9) Nanographite structure
- the structure recognition peptide is a peptide capable of binding to a nanographite structure containing all or part of the amino acid sequence represented by any of SEQ ID NOs: 1 to 20, wherein (1) to ( 7) The nanographite structure according to any one of the above, or (10) the amino acid arrangement IJ shown in any one of SEQ ID NOs:!
- DYFSSPYYEQLF SEQ ID NO: 1
- the nanographite structure metal nanoparticle composite according to (8) or (9) above, or (11) the amino acid arrangement IJ represented by any one of SEQ ID NOs: 1 to 20 is YDPFHII (SEQ ID NO: 2)
- the nanographite structure metal nanoparticle composite according to the above (8) or (9), or (12) nanoparticles of an inorganic metal atom or an inorganic metal compound are metal nanoparticles.
- nanographite structure according to any one of the above (1) to (11), characterized in that the metal nanoparticle composite Or a nanographite structure according to any one of the above (1) to (: 11), wherein the nanoparticles of the inorganic metal atom or inorganic metal compound are metal compound nanoparticles.
- Nanographite structure The nano-black lead structure-metal-nanoparticle composite according to any one of (1) to (19) above, wherein (21) the metal nanoparticle is a substrate
- the present invention is also characterized in that (23) a protein obtained by fusing or chemically binding a nanographite structure recognition peptide to the surface of a cage protein, or (24) the cage protein is a ferritin protein family.
- the protein according to (23) above, (25) the protein according to (24) above characterized by being a ferritin of the ferritin protein family, and (26) ferritin derived from higher eukaryotes
- the protein according to (25) above, or (27) the ferritin derived from higher eukaryotes is L-type ferritin derived from equine spleen.
- the protein or (28) the ferritin protein family is derived from bacteria, or the protein according to (23) above, or (29) a cage protein force virus particle.
- the protein according to (23) above and (30) the nanographite structure-recognizing peptide are peptides having an amino acid sequence represented by any one of SEQ ID NOs:! -20.
- the protein according to any one of the above (23) to (29) and (31) the nanographite structure recognition peptide may include all or part of the amino acid sequence represented by any one of SEQ ID NOs: 1 to 20. Any of the above (23) to (29), wherein the peptide has a binding ability to the nanographite structure containing The protein according to (30) to (31) above, wherein the protein according to (32) SEQ ID NO :!
- the nanoparticle of a metal atom or an inorganic metal compound is a metal nanoparticle, or the protein according to any one of (23) to (33) above, (35) an inorganic metal atom or an inorganic metal
- the nanoparticle of the compound is a metal compound nanoparticle, or the protein according to any one of the above (23) to (33), or (36) the nanoparticle force of the metal compound is an oxidized metal nanoparticle
- the protein according to (35) above, which is a magnetic material nanoparticle, and (38) the metal is iron, beryllium, gallium, manganese, phosphorus, uranium, lead, cononoreto, nickelore,
- nanographite structure is a carbon nanotube or carbon nanohorn
- protein is a particle, zinc selenide nanoparticle, zinc sulfide nanoparticle, or cadmium sulfide nanoparticle
- the present invention provides (42) a nanographite structure-recognizing peptide nanoparticle by retaining nanoparticles of an inorganic metal atom or an inorganic metal compound in the internal space of the protein according to any one of (23) to (41).
- a method of supporting a plurality of nanoparticles of an inorganic metal atom or an inorganic metal compound on a nanographite structure using affinity with a graphite structure, or (43) any one of (23) to (41) above By holding the inorganic metal atom or inorganic metal compound nanoparticles in the inner space of the protein and utilizing the affinity of the nanographite structure recognition peptide with the nanographite structure, the nanographite structure has an inorganic metal atom or A method of making a composite of nanographite structure and inorganic metal compound nanoparticles by supporting a plurality of inorganic metal compound nanoparticles and removing the protein portion by heat treatment; ) Inorganic nanoparticles or inorganic metal compound nanoparticles are
- a method of forming a composite of nanographite structure and inorganic metal compound nanoparticle by loading multiple atoms or inorganic metal compound nanoparticles and removing the protein part by electron beam treatment (45) A method of binding a nanographite structure to the protein described in any one of the above (23) to (41) and arranging the nanographite structure, or (46) a method of forming a two-dimensional array ( The present invention relates to a method for binding a nanographite structure to the protein according to any one of 23) to (41) and arranging the nanographite structure.
- FIG. 1 is a diagram showing the crystal structure of L-type ferritin (LF) derived from equine spleen and the site where DYFSSPYYEQLF (SEQ ID NO: 1; N1 sequence) is presented.
- the N-terminal part of the crystal structure of equine spleen-derived L ferritin (LF0) is shown in red.
- Fig. 1 since the N-terminus of LF0 is located outside the molecule, it is possible to present multiple N1 sequences by fusing the N1 sequence to the N-terminus.
- FIG. 2 is a schematic diagram of the construction of an N1-LF expression vector pKIS2.
- the N1-LF recombinant ferritin expression vector PKIS2 was prepared by cleaving pKITO, an L ferritin expression vector derived from equine spleen, with restriction enzymes BamHI and Sacl, and inserting the annealed synthetic DNAs of SEQ ID NOS: 22 and 23. Cut with BamHI. A short DNA fragment produced when pKITO was cleaved with BamHI was inserted into it.
- FIG. 3 shows the results of polyacrylamide gel electrophoresis of the final purified sample of N1-LF.
- the uniformity of 3 ⁇ g of N1-LF final purified sample was evaluated by polyacrylamide gel electrophoresis.
- a protein band was observed only at the position corresponding to the molecular weight of the target N1-LF. It is a highly pure standard.
- the molecular weight marker on the left lane corresponds to 97.4, 66.3, 42.4, 30.0, 20.1, 14.4 kDa.
- the right lane is the Nl-LF final purified sample.
- FIG. 4 is a diagram showing the formation of iron oxide nanoparticles in the internal space of N1-LF.
- N1 The state of the solution when iron oxide nanoparticles are formed in the internal space of LF.
- Control ferritin Does not contain protein solution. From the color of the solution, it can be seen that iron oxide nanoparticles were formed in the internal space of ferritin.
- FIG. 5 is a transmission electron micrograph showing that iron oxide nanoparticles are formed in the internal space of N1-LF.
- N1-LF images stained with 1% gold gnolecose were observed with JEOL JEOL1010, lOOkV.
- FIG. 6 is a transmission electron micrograph showing that iron oxide nanoparticles are formed in the internal space of LF0. N1-LF images stained with 1% gold gnolecose were observed with JEOL J EOL1010, lOOkV.
- FIG. 7 is a diagram showing metal oxide nanoparticles supported on carbon nanohorns.
- Nl_LF was able to specifically carry multiple metal oxide nanoparticles on carbon nanohorn (left).
- L type ferritin (LF0) derived from horse spleen cannot support metal oxide nanoparticles on carbon nanophones (right).
- FIG. 8 is a view showing metal oxide nanoparticles supported on single-walled carbon nanotubes.
- the nanographite structure-metal nanoparticle composite of the present invention includes an inorganic metal atom or an inorganic metal in the internal space of a protein in which a nanographite structure recognition peptide is fused or chemically bonded to the cage protein surface.
- the nanographite structure carries a plurality of inorganic metal atoms or inorganic metal compound nanoparticles.
- the protein is not particularly limited as long as it is a complex, and the protein is not particularly limited as long as it is a protein in which a nanographite structure recognition peptide is fused or chemically bound to the surface of a cage protein.
- the cage protein of the present invention refers to a protein having a space inside and having a substance-encapsulating ability.
- the cage protein is derived from the ferritin protein family and bacteria. And virus particles.
- the ferritin protein family include ferritin and apoferritin.
- L type or H type ferritin derived from higher eukaryotes such as L type ferritin derived from equine spleen is preferably exemplified.
- Can do examples of cage proteins derived from bacteria include DpsA protein and MrgA protein.
- virus particles include virus particles such as retroviruses, adenoviruses, rotaviruses, polioviruses, cytomegaloviruses and cauliflower mosaic viruses.
- nanographite structure in addition to carbon nanotubes and carbon nanohorns, functional groups such as amino groups, hydroxyl groups, and carboxyl groups are added to the carbon structure that is formed by powerful carbon nanotubes and carbon nanohorns.
- functional groups such as amino groups, hydroxyl groups, and carboxyl groups are added to the carbon structure that is formed by powerful carbon nanotubes and carbon nanohorns.
- Such modified nanographite structures can also be listed.
- nanographite structure recognition peptide examples include peptides consisting of amino acid sequences IJ shown in SEQ ID NOs:! -20 (see Patent Document 3 and Non-Patent Document 8), and SEQ ID NOs:! -20.
- DYFSSPYYEQLF (SEQ ID NO: 1) and Y DPFHII (SEQ ID NO: 2) peptides are preferred, among which the ability to list peptides capable of binding to a nanographite structure containing all or part of the amino acid sequence indicated by the force Can be exemplified.
- the site of the cage protein surface where the nanographite structure recognition peptide is fused or chemically bound is not particularly limited as long as the nanographite structure recognition peptide can bind to the nanographite structure.
- a loop structure site exposed on the ferritin surface can be exemplified in addition to the amino terminal.
- Nanoparticles of the inorganic metal atom or inorganic metal compound include nanoparticles of metals such as iron, beryllium, gallium, manganese, phosphorus, uranium, lead, cobalt, nickel, zinc, cadmium, chromium, and the like.
- Forces that can include metal oxide nanoparticles such as metal oxides, hydroxides, carbonates, and metal compound nanoparticles such as magnetic material nanoparticles, iron oxide nanoparticles, cadmium selenide nanoparticles, selenium-zinc nanoparticles, Zinc sulfide nanoparticles and cadmium sulfide nanoparticles can be suitably exemplified.
- a protein having a nanographite structure-recognizing peptide fused or chemically bound to the surface of the cage protein is further fused or chemically bound to a functional peptide such as a peptide capable of binding to titanium. It is also possible to arrange nanographite structures on a titanium substrate and to carry a plurality of nanoparticles on the nanographite structure.
- a nanometer-size pattern can be prepared by two-dimensionally crystallizing the cage protein.
- nano-graphite structure recognition peptides can be retained by holding inorganic metal atoms or inorganic metal compound nanoparticles in the internal space of proteins in which nano-graphite structure recognition peptides are fused or chemically bound to the surface of cage proteins.
- the nanographite Nano-graphite structure in which structure is two-dimensionally aligned on substrate Metal nano-particle composite, or nano-graphite structure in which metal nano-particle is two-dimensionally aligned on substrate Obtain metal nano-particle composite Can do.
- the obtained composite can be a basic technology for high integration of device 'memory' in the semiconductor field such as device 'memory'.
- the cage protein is obtained by utilizing the reactivity between a compound having a plurality of functional groups such as dartal aldehyde and the side chain of the amino acid constituting the protein.
- a method of immobilizing proteins on the substrate by forming a cross-link between them and a SAM with a functional group (a molecule that has a membrane self-forming ability) is placed on the substrate, and the functional group and By forming a bond between the side chains of the amino acids that make up the protein and immobilizing the protein, etc.
- nanographite structures in which nanographite structures are aligned two-dimensionally on a substrate metal nanoparticle composites, and nanographite structures in which metal nanoparticles are aligned two-dimensionally on a substrate You can get a body.
- the resulting composite can be a basic technology for highly integrated devices such as device memory in the semiconductor field.
- Fusion ferritin protein (N1-LF, Fig. 1) expressed by fusion of L-type ferritin (LF) derived from horse spleen with nanographite structure recognition peptide (N1) consisting of amino acid sequence shown in SEQ ID NO: 1.
- the DNA (pKIS2) for the preparation was prepared according to the following procedure. In other words, Met, which is complementary to each other and is the start codon, is encoded by the amino acid sequence shown in SEQ ID NO: 21, with the restriction enzyme B HI linker sequence on the start codon side and the restriction enzyme Sail linker on the opposite side.
- the annealing reaction was performed by slowly returning to room temperature.
- the plasmid pKIT ⁇ (Okuda et al. 2003, Biotechnology and Bioengineering, Vol 84, No. 2, pl87-194) cloned downstream of the t-type promoter of L-type ferritin cDNA from horse spleen was used as a restriction enzyme. Dissolve with BamHI, Sail, 1% agarose gel electrophoresis, isolate a large DNA fragment of approximately 6 kb using the Gene Clean II kit (BIO101), mix with the annealed DNA described above, and add T4 DNA ligase. And combined.
- this DNA and pKITO were each digested with BamHI and separated by 1% agarose gel electrophoresis.
- the former was an approximately 6 kb fragment, and the latter was an approximately 300 bp fragment.
- Gene Clean II kit BIO101
- T4 DNA Ligase T4 DNA Ligase
- the bound DNA was transferred to Escherichia coli XLI-blue strain (hsdR17, supE44, recAl, end Al, gyrA46, thi, relAl, lac / F '[proAB +, lad q A (lacZ) M15:: Tnl0 (tetR)]) 'Suspension' method (Molecular Cloning Third Edition, Cold Spring Harbor Laboratory Press) (clone, cloned, primer in about 300 bp BamHI fragment of pKITO (SEQ ID NO: 2 Using 4), the DNA sequence was used to determine the clones in which the BamHI fragment of about 300 bp was inserted in the desired direction by the dye oxidation termination method (CEQ DTCS Quick start kit, Beckman, California). For migration of reaction products and data analysis, an auto-cabilizer sequencer (CEQ2000, Beckman) was used. ( Figure 2)
- Escherichia coli XLI_blue was transformed into pKIS2 according to a conventional method, and the colonies were picked up with a sterilized toothpick and cultured in 5 ml of LB medium at 37 ° C for 16-18 hours. Next, this culture medium was transplanted to 1 ⁇ medium at 11 6 and incubated at 37 ° for 16 to 18 hours. E. coli was collected by centrifugation (Beckman J2-21M, JA-14 rotor, 5000 rpm, 5 minutes). The collected E.
- E. coli disruption solution was obtained.
- the E. coli disruption solution is centrifuged (Kubota, 5922, RA410M2 rotor, 8000 rpm, 30 minutes) to recover the soluble fraction and denature the contaminating protein by warm bathing at 65 ° C for 20 minutes. It was. Centrifugation (Kubota, 5922, RA410M2 rotor, 8000 rpm, 30 minutes) removed the denatured contaminating proteins that formed a precipitate, and the supernatant was collected.
- the precipitate was dissolved in 20 ml of 50 mM Tris_HCl (pH 8.0), this time 5M NaCl was added to a final concentration of 0.375 M, stirred, allowed to stand at room temperature for 5-10 minutes, and then centrifuged ( The precipitate was recovered by Kubota, 5922, RA410M2 rotor, 5000 i "pm, 10 minutes. The recovered precipitate was dissolved in 10 ml of 50 mM TrisHCl (pH 8.0). [0030] Furthermore, purification by gel filtration chromatography is performed as necessary.
- the above purified sample 200-500 ⁇ 1 was injected into a SW4000XL column (TOSO) equilibrated with 50 mM TrisHCI (pH 7.5), 150 mM Na CI, ImM NaN3 and chromatographed at a flow rate of lml / min. Separation and purification by the method described above, the fraction corresponding to ferritin 24-mer was recovered (Fig. 3).
- N1-LF obtained in Example 1 was confirmed to have the ability to form iron oxide nanoparticles in the internal space by the following procedure.
- Example 1 a recombinant was used for L-type ferritin (LF0) derived from horse spleen.
- the recombinant was prepared as follows. Escherichia coli XLI_blue was transformed with pKITO according to a conventional method, and the colonies were picked up with a sterilized toothpick and cultured in 5 ml of LB medium at 37 ° C for 16-18 hours. Next, this culture solution was transferred to 1 liter LB medium and cultured at 37 ° C for an additional 16-18 hours. E. coli was collected by centrifugation (Beckman J2 — 21M, JA-14 rotor, 5000 rpm, 5 minutes). The collected E.
- coli was washed with 80 ml of 50 mM Tris-HCl (pH 8.0) and centrifuged again (Kubota, 5922, RA410M). Bacteria were collected using 2 rotors, 4000 rpm, 10 minutes). After suspending in 30 ml of 50 mM Tris-HCl (pH 8.0), using an ultrasonic breaker (BRANSON, SONIFIER 250, trace chip, maximum output, duty cycle 50%, 2 minutes 3-4 times) Then, E. coli disruption solution was obtained. The soluble protein fraction is recovered by centrifugation (Kubota, 5922, RA410M2 rotor, 8000 rpm, 30 minutes) of the E.
- BRANSON, SONIFIER 250 ultrasonic breaker
- N1-LF (SEQ ID NO: 26) having the iron oxide nanoparticles obtained in Example 2 in the internal space specifically binds to the nanographite structure, but the iron oxide obtained in Comparative Example 2
- LF0 SEQ ID NO: 25
- This carbon nanohorn is 0.1 mg fetal bovine serum albumin 0.05% Polyoxyethylenesorbitan monolaurate [hereinafter "I ween-20 (Nkuma, 3 ⁇ 4t. Loui s)] containing TBS (hereinafter TBS)
- TBS Polyoxyethylenesorbitan monolaurate
- the carbon nanohorn was precipitated by centrifugation (Kubota, 5922, AT-2018M rotor, 15000 rpm, 15 minutes) and suspended in TBS-BT to 1 mg / ml. After repeating the operation three times, the precipitated carbon nanohorn was suspended in N1-LF or LF0-containing TBS-BT having 0.1 mg / ml iron oxide nanoparticles in the core so that the concentration was 1 mg / ml.
- Hipco Carbon Nanotechnology, Texas
- a single-walled carbon nanotube synthesized by chemical vapor deposition was treated at 1750 ° C, C ⁇ — 5 Torr for 5 hours, and then in 70% concentration of nitric acid.
- the mixture was refluxed at about 130 ° C for 30 minutes. After completion, the mixture was neutralized with sodium hydroxide and washed with distilled water to prepare a single-walled carbon nanotube having a functional group (including a carboxyl group).
- This single-walled carbon nanotube was dissolved in TBS-BT in the same manner as in Example 3.
- Single-walled carbon nanotubes are centrifuged (Kubota, 5922, AT— 2018M rotor, 150 OOrpm, 15 minutes) and suspended again with TBS-BT. After this operation was repeated three times, the precipitated single-walled carbon nanotubes were suspended in N1-LF-containing TBS-BT having iron oxide nanoparticles in the core in the same manner as in Example 3. The mixture was rotated and stirred for 12 hours at room temperature using rotat or RT_50 manufactured by Taitec.
- single-walled carbon nanotubes were precipitated by centrifugation (Kubota, 5922, AT—2018M rotor, 15000 rpm, 15 minutes) and washed 5 times with 0.05% Tween20-containing TBS. After that, the desalted water was replaced with sterilized water, and observed with a transmission electron microscope (T ⁇ PC ⁇ N EM-002 B, 120kV).
- T ⁇ PC ⁇ N EM-002 B, 120kV transmission electron microscope
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JP2006549069A JP4843505B2 (ja) | 2004-12-24 | 2005-12-22 | ナノ黒鉛構造体−金属ナノ粒子複合体 |
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- 2005-12-22 KR KR1020077016330A patent/KR100905526B1/ko active IP Right Grant
- 2005-12-22 EP EP05819764A patent/EP1840082B1/en not_active Not-in-force
- 2005-12-22 WO PCT/JP2005/023675 patent/WO2006068250A1/ja active Application Filing
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Also Published As
Publication number | Publication date |
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US8017729B2 (en) | 2011-09-13 |
EP1840082A1 (en) | 2007-10-03 |
JP4843505B2 (ja) | 2011-12-21 |
CA2592180A1 (en) | 2006-06-29 |
US20100029910A1 (en) | 2010-02-04 |
JPWO2006068250A1 (ja) | 2008-08-07 |
EP1840082A4 (en) | 2011-06-01 |
CA2592180C (en) | 2010-02-09 |
EP1840082B1 (en) | 2012-07-18 |
KR20070089236A (ko) | 2007-08-30 |
KR100905526B1 (ko) | 2009-07-01 |
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