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  • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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  • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
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  • A01K67/0276—Knock-out vertebrates
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  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
  • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
  • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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  • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
  • C12N5/06—Animal cells or tissues; Human cells or tissues
  • C12N5/0602—Vertebrate cells
  • C12N5/0603—Embryonic cells ; Embryoid bodies
  • C12N5/0604—Whole embryos; Culture medium therefor
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  • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
  • C12N5/10—Cells modified by introduction of foreign genetic material
  • C12N5/12—Fused cells, e.g. hybridomas
  • C12N5/16—Animal cells
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2207/00—Modified animals
  • A01K2207/12—Animals modified by administration of exogenous cells
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
  • A01K2227/00—Animals characterised by species
  • A01K2227/10—Mammal
  • A01K2227/105—Murine
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  • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
  • C12N2501/20—Cytokines; Chemokines
  • C12N2501/23—Interleukins [IL]
  • C12N2501/235—Leukemia inhibitory factor [LIF]
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  • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
  • C12N2501/40—Regulators of development
  • C12N2501/415—Wnt; Frizzeled
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  • C12N2510/00—Genetically modified cells
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  • C12N2517/00—Cells related to new breeds of animals
  • C12N2517/02—Cells from transgenic animals
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  • C12N2799/00—Uses of viruses
  • C12N2799/02—Uses of viruses as vector
  • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
  • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

Definitions

• the invention is directed to improved methods for generating an animal homozygous for a genetic modification. More particularly, the present invention is directed to methods for increasing the efficiency of generating animals capable of transmitting a desired modification to subsequent offspring, for generating animals having high genetic material contribution from donor cells, and for increasing the efficiency and decreasing the time required for generating homozygous animals carrying the desired genetic modification.
• Patent No. 6,586,251 Methods for generating transgenic animals are also known in the art. A proposed method for improving the efficiency of generating animals capable of transmitting a desired modification in TT2 ES cells is described in Yagi et al. (1993) Analytical Biochemistry
• the invention is based in part on the realization that introduction of a modified cell into an early stage host embryo substantially increases the genetic material and cellu lar contribution of the donor cells to the host embryo, such that the animal produced has increased fraction of cells from the donor and thus has an increased likelihood of transmitting the modification through the germline. Further, the methods of the invention allow a broader range of donor cells to be used than was practical with prior art methods. The methods of the instant invention thus reduce the number of animals and breedings necessary to generate an animal homozygous for a modification.
• the invention features a method for generating a mod ified mammal, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo; and (b) introducing the embryo of (a) into a surrogate mother for gestation, wherein a modified mammal is generated.
• the early stage embryo is a pre-morula stage cell.
• the early stage embryo is an 8-cell embryo.
• the early stage embryo may be derived from any strain.
• an inbred embryo e.g., C57BL/6
• an outbred embryo e.g., Swiss Webster, is used as a host embryo.
• the eukaryotic donor cell is a stem cell. More specifically, the stem cell is an embryonic sterrf(E?S) bell 6f ah" €S :ji like'Ml. ⁇ l S"fc ⁇ l1s from any suitable ES cell line can be used in the method of the invention.
• the ES cell is derived from an inbred strain, e.g., 129, C57BL/6, or BaIbC.
• the ES cell is derived from a hybrid strain, e.g., C57BL/6 x 129.
• the cell is a mouse ES cell.
• the method of the invention may be practiced with cells derived from other mammals, e.g., a rat ES or ES-Iike cell.
• the ES or ES-Iike cell is a modified cell comprising a genetic modification.
• the modification may arise spontaneously, may be random, or may result from experimental manipulation, for example, by the introduction of a foreign nucleic acid.
• a foreign nucleic acid may be introduced by any method known to the art, e.g., by homologous recombination.
• the invention features a method for generating a mammal homozygous for a genetic modification, comprising: (a) introducing a female eukaryotic donor cell into an early stage host embryo, wherein the eukaryotic donor cell is heteroz/gous for the genetic modification; (b) maintaining the embryo of (a) in culture for further development; (c) introducing the embryo of (b) into a surrogate mother for gestation, wherein a modified female mammal is generated; (a') introducing a male eukaryotic donor cell into an early stage host embryo, wherein the eukaryotic donor cell is heterozygous for the genetic modification; (b 1 ) maintaining the embryo of (a 1 ) in culture for further development; (c 1 ) introducing the embryo of (b 1 ) into a surrogate mother for gestation, wherein a modified male mammal is generated; and (d) breeding the sexually mature female and male mammal of (c and c'), where
• the female eukaryotic donor cell of step (a) is derived from the same cell line of the male eukaryotic donor cell of step (a 1 ).
• the female eukaryotic donor cell of step (a) is an XO cell and the modified female mammal of step (c) is an XO mammal.
• the male or female eukaryotic donor cell is a stem cell, preferably a heterozygous ES or ES-Iike cell.
• the heterozygous ES cell is generated by (i) obtaining a large cloned genomic fragment containing a DNA sequence of interest; (ii) using bacterial homologous recombination to genetically modify the large cloned genomic fragment of (i) to create a large targeting vector for use in the eukaryotic donor cells (LTVEC, or large targeting vector); (iii) introducing the LTVEC of (ii) into the eukaryotic donor cells to modify an endogenous gene or chromosomal locus in the eukaryotic donor cells; and (iv) using a quantitative assay to detect modification of allele in the eukaryotic donor cells of (iii) to identify those eukaryotic donor cells in which the endogenous gene or chromosomal locus has been genetically modified.
• the culture conditions of step (b) and/or (b 1 ) allow the embryo to develop to a post-morula stage or further.
• the culture conditions of step (b) and/ ⁇ r(b') are prdvldecF'by a ':; grbwtH ';; fck!itor-conditioned culture medium.
• the growth factor is a protein of the Wnt family.
• the Wnt protein is added to the culture medium directly.
• the Wnt protein is produced by mouse L-cell.
• the culture medium further comprises a leukemia inhibitor factor (LIF).
• LIF leukemia inhibitor factor
• a blastocyst stage embryo of (b) and/or (b 1 ) is introduced into a surrogate mother for gestation and further development.
• the eukaryotic donor cell may be introduced into an early stage embryo by any method known to the art.
• the eukaryotic donor cell is introduced under the zona pellucida of the early stage embryo.
• Other methodologi es include removal of the zona pellucida membrane.
• the eukaryotic donor cell may be introduced under the zona pellucida by injection or other methods that create an opening in the zona pellucida.
• an opening is created in the zona pellucida by a laser.
• the laser comprises an Infrared Solid State Diode laser.
• the eukaryotic donor cell is introduced into the host embryo by cell fusion methods.
• a modified mammal comprising more than 90% donor cell-derived cells is bred in step (d).
• the mod ified mammal comprises more than 95% donor cell-derived cells. More preferably, the modified mammal comprises 100% donor cell-derived cells.
• the invention features a method of generating an embryo homozygous for a genetic modification, comprising: (a) introducing a female eukaryotic donor cell into an early stage host embryo, wherein the female eukaryotic donor cell is heterozygous for the genetic modification; (b) maintaining the embryo of (a) in culture for further development; (c) introducing the embryo of (b) into a surrogate mother for gestation, wherein a modified female mammal is generated; (a') introducing a male eukaryotic donor cell into an early stage host embryo, wherein the male eukaryotic donor cell is heterozygous for the genetic modification; (b') maintaining the embryo of (a 1 ) in culture for further development; (c 1 ) introducing the embryo of (b 1 ) into a surrogate mother for gestation, wherein a modified male mammal is generated; and (d) breeding the modified female and the modified male mammal of (c and c'), wherein a
• the invention features a method of generating a mammal homozygous for a genetic modification, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo, wherein the eukaryotic donor cell is homozygous for the genetic modification; (b) maintaining the embryo of (a) in culture for further development; (c) introducing the embryo of (b) into a surrogate mother for gestation, wherein a mammal homozygous for the genetic modification is generated.
• the invention features a method of generating an embryo homozygous for genetic modification, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo, wherein the eukaryotic donor cell is homozygous for the genetic modification; and (b) culturing the early stage embryo of step (a), wherein an embryo homozygous for the genetic modification is generated.
• the invention features a method of increasing the relative contribution of genetic material from a donor eukaryotic donor cell to a host embryo, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo; and (b) culturing the early stage embryo of step (a) under conditions that allow the embryo to develop to a post-morula stage or further.
• the culture conditions of step (b) allow the embryo to develop to a blastocyst.
• the culture conditions of step (b) allow the embryo to develop to a gastrula or beyond.
• the culture conditions of step (b) allow the embryo to develop to a post-morula stage or further.
• the culture conditions of step (b) is provided by a growth factor-conditioned culture medium.
• the growth factor is a protein of the Wnt family.
• the Wnt protein is added to the culture medium directly.
• the Wnt protein is produced by L-cells.
• the culture medium further comprises a leukemia inhibitor factor (LIF).
• LIF leukemia inhibitor factor
• the invention features a method of generating a mammal having an increased relative contribution of genetic material from a donor eukaryotic donor cell to a host embryo, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo; (b) culturing the early stage embryo of step (a) under conditions that allow the embryo to develop to a post- morula stage or further; and (c) introducing the embryo of (b) into a surrogate mother for gestation, wherein a mammal having an increased fraction of cells derived from the eukaryotic donor cell is generated.
• the invention features a method of generating a mammal capable of transmitting a genetic modification, comprising: (a) introducing a eukaryotic donor cell into an early stage embryo, wherein the eukaryotic donor cell carries a genetic modification; (b) culturing the early stage embryo of step (a) under conditions which allow the embryo to develop to a post-morula stage or further; and (c) introducing the embryo of (b) into a surrogate mother for gestation, wherein a mammal capable of transmitting the genetic modification is generated.
• the invention features a genetically modified mammal generated by a method of the invention.
• 90% or more of the cells of the mammal are donor cell-derived cells; more preferably, 95% or more of the cells of the mammal are donor cell- derived cells; even more preferably, 99% or more of the cells of the mammal are donor cell-derived cells.
• IFoile 'eitibOiti'ii+ieht, 1 ⁇ 50%- ; ⁇ fith&''cells of the rnammal are donor cell-derived cells.
• the invention features a genetically modified embryo generated by a method of the invention.
• 90% or more of the cells of the embryo are donor cell-derived cells; more preferably, 95% or more of the cells are donor cell-derived cells; even more preferably, 99% or more of the cells are donor cell-derived cells.
• 100% of the cells of the embryo are donor cell-derived cells.
• the invention features a culture medium for maintaining and/or growing an early stage embryo, wherein the culture medium comprises a growth factor.
• the growth factor is a protein of the Wnt family.
• the Wnt family protein is selected from the group consisting of Wnt1 , Wnt3a, Wnt2, Wnt2b, Wnt3, Wnt4, Wnt5a, Wnt6,
• the Wnt protein is Wnt3a.
• the Wnt protein is a recombinant protein.
• the culture medium is conditioned by a Wnt-producing mouse L-cell.
• the culture medium further comprises a maintenance agent.
• the maintenance agent is a leukemia inhibitor factor (LIF)
• embryonic stem (ES)-like cell includes a cell which, upon introduction into an embryo, can contribute to any tissue of the developing embryo.
• the terms "increased contribution”, “higher relative percentage” and the like, include an improved genetic material contribution of a donor eukaryotic donor cell to an organism resulting from further development of a modified early stage host embryo.
• the method of the invention provides the means of increasing the probability that cells introduced into the host embryo contribute to all tissues, including germline tissues, of the generated animal.
• gene knockout as used herein is meant a genetic modification resulting from the disruption of the genetic information encoded at a chromosomal locus.
• gene knockin as used herein is meant a genetic modification resulting from replacement of genetic information encoded at a chromosomal locus with a different DNA sequence or insertion of foreign genetic information at a chromosomal locus.
• knockout animal as used herein is meant an animal in which a significant proportion of the animal's cells harbor a gene knockout.
• knockin animal as used herein is meant an animal in which a significant proportion of the animal's cells harbor a genetic knockin.
• One of the desired components of a transgenic animal study is generating a genetically modified transgenic animal capable of transmitting the genetic modification to progeny, i.e., a transgenic animal comprising the genetic modification in its germline.
• Current methods of creating such a transmission-capable transgenic animal tend to be inefficient in terms of resources and time expenditures.
• a modified ES cell heterozygous for a desired genetic modification is injected into a recipient blastocyst embryo, and the recipient embryo is implanted into a surrogate mother for gestation and birth of transgenic progeny.
• the resulting transgenic progeny are chimeric because some of the progeny's tissues are derived from the injected ES cells while other of the progeny's tissues are derived from the recipient embryo cells. Because of this chimerism, the injected ES cells comprising the genetic modification may or may not form germline tissues in the progeny and be capable of transmitting the genetic modification to the next generation. To determine whether a chimera is capable of transmitting the genetic modification, the chimera must be bred to another animal that does not carry the same genetic modification to establish if the desired modification is transmitted to the resulting progeny (F1 ).
• Detection of the desired genetic modification in the F1 progeny of the cross between the chimera and the other animal establishes that the chimera carries the desired genetic modification in its germline and is capable of transmitting the modification to its progeny (germline transmission). Typically, approximately 50% of chimeras exhibit germline transmission. Coat color is the most frequS ⁇ ly ds ⁇ d i
• the current need to generate an F1 ge neration to determine if the chimera is capable of transmitting the genetic modification is inefficient and costly in terms of time and the costs of breeding and maintaining F1 progeny.
• One method of improving the efficiency of the process for generating transgenic animals is provided by the instant invention which allows introduction into pre-morula embryos of cells that generate animals having an increased genetic material contribution of the exogenous cells relative to the results obtained when donor cells are introduced into later stage embryos, e.g., blastocysts.
• a much higher percentage of the FO animals are germline transmitters. In most instances, FO animals are 100% germline transmitters and thus these FO animals can transmit ES cell materials to all of their offspring
• Introducing donor cells into early stage host embryos provides several important benefits over the current methods, which teach the use of later stage host embryos, e.g., blastocysts.
• later stage host embryos e.g., blastocysts.
• the number of early stage embryos harvested from a donor mother e.g., female BL/6 mouse
• the number of later stage embryos harvested is hig her than the number of later stage embryos harvested.
• fewer pregnant female mice are needed as donors, decreasing the cost of obtaining and maintaining pregnant female mice.
• donor cells may be introduced into a smaller number of early stage host embryos than later stage embryos to generate the same number of chimeric animals, red ucing the time and cost of introducing donor cells into embryos, e.g., when introduction is by microinjection, the amount of time spent on microinjections is greatly reduced.
• the instant invention also allows cultu ring the donor cell-containing host embryo to post- morula stages, e.g., to a blastocyst stage or a gastrula stage, before being introduced into a surrogate mother for gestation.
• the resulting embryo Since in vitro culturing conditions are more favorable for the donor cells than for the host embryo, the resulting embryo has a higher content of donor cell-derived cells compared to the method by which the morula stage host embryo is introduced into a surrogate mother for gestation.
• An important improvement provided by the instant invention is that the number of animals generated that are capable of transmitting donor DNA is increased substantially with the use of early stage host embryos, such that an entire generation of breeding is eliminated. This is a significant practical improvement with important commercial implications.
• a method known in the art that allows an entire generation of breeding to be eliminated employs tetraploid embryos as recipients of the modified donor cells. As the tetraploid cells of the recipient embryo are incapable of contributing to the tissue of the developing animal, the animals that are born are completely derived from the modified donor cells.
• the method of the invention can be applied to introduce inbred ES cells into outbred recipient embryos through microinjection.
• Mouse embryos for injection were obtained from natural matings where the morning of the plug was designated as 0.5 days p.c. (post coitus). Oviducts were collected from plugged females at 2.5 days p.c. and were flushed with Dulbecco's medium to harvest the 8-cell embryos. The embryos were maintained in this medium for culture (37 0 C in a 5% CO 2 incubator) and microinjection procedures.
• the injection of the 8-cell embryos was performed using standard microinjection techniques except that prior to introduction of the ES cells, a hole was generated in the zona pellucida of the 8- cell embryo using an XY Clone laser system according to the manufacturer's specifications. The injection needle is inserted through this hole and 6 to 10 ES cells are deposited into the perivitelline space.
• Homologous DLL4 knockout ES cells were microinjected into an 8-cell mouse embryo as described above. Injected embryos were cultured to blastocyst stage and were transferred to a surrogate female for gestation. All DLL4 knockout embryos died during gestation. The cause of death was identical to that observed for embryos produced by conventional breeding of heterozygous mice. Observation of the null DLL4 phenotype in the FO generation avoided the two generations of breeding that would normally be required to generate DII4 null mice.
• Percentages of ES cell contribution were estimated by the coat color of FO mice.
• Example 4 Generation of Highly and Fully ES Cell-Derived Female FO Mice
• Genetically modified XO ES cells (648B-H12 clone) were microinjected into either 8-cell or blastocyst stage C57BL/6 embryos. The microinjected blastocyst embryos were transferred to a surrogate female for gestation immediately after the injection. The microinjected 8-cell embryos were further cultured in KSOM+AA culture medium to blastocyst stage and were then transferred to a surrogate female for gestation. The percentages of ES cell contribution in the female FO mice were estimated by their coat color.
• Unmodified male inbred (C57BL/6) or hybrid (F1 H4) ES cells were microinjected into either 8-cell stage or blastocyst stage Swiss Webster (SW) embryos.
• the microinjected blastocyst embryos were transferred to a surrogate female for gestation immediately after the injection.
• the microinjected 8-cell embryos were further cultured in KSOM+AA culture medium to blastocyst stage and were then transferred to a surrogate female for gestation. Results are summarized in Table 4 (* Percentages of ES cell contribution were estimated by the coat color of FO mice).
• Example 6 Highly and Fully ES Cell-Derived FO Mice are Germline Transmission Competent
• F1 H4-derived male FO mice having more than 90% cells derived from the ES cells (more than 90% ES cell contribution) were used to test germline transmission competency.
• male ES cells sexually mature male FO mice were bred with sexually mature females.
• the coat color of the offspring was used as a marker for the evaluation of germline transmission competency. Results are summarized on Table 6. When 8-cell stage embryos were used as host embryos, over 95% (51 out of 53) of the FO males generated by 8-cell microinjection exhibited 100% germline transmission competency.
• Example 7 Effect of Post-lVlicroinjection Culture Medium on the Quality of FO Mice
• Modified male C57BL/6 and F1 H4 ES cells were microinjected into 8-cell stage Swiss Webster (SW) embryos.
• SW Swiss Webster
• the microinjected embryos were cultured in different culture media to blastocyst stage and were then transferred to a surrogate female for gestation.
• Three different culture media were used: (1) KSOM, mouse embryo culture medium; (2) LIF, ES cell cultu re WntSrWrfO'conditioned ES cell culture medium. Results are summarized in Table 7.
• Wnt3 conditioned ES medium was produced as following: (i) mouse L-cells were plated into a T75 flask in medium made of high glucose DMEM, 1O% FBS, and L-glutamine, and were incubated at 37°C, with 5% CO 2 ; (ii) when cell density reached 100% confluency, 10% of the cells were re-plated into another T75 flask; (iii) the culture medium was colleted until cell density reached confluency again (about 3 days after re-plating); and finally, (iv) the collected culture medium was mixed with equal volume of ES cell culture medium without LIF but with serum replacement (* Percentages of ES cell contribution were estimated by the coat color of FO mice).
• Example 8 Generation of Animals Homozygous for a Genetic Modification from Heterozygous ES cells.
• Male ES cells heterozygous for a desired genetic modification are microinjected into an 8- cell mouse embryo as described in Example 1.
• Female ES cells derived from the same male ES cell line and heterozygous for the same genetic modification are microinjected into another 8-cell mouse embryo as described above. Both embryos are cultured to the blastocyst stage and are transferred to a surrogate female for gestation. The resulting germline transmitting male and female FO mice are bred to obtain progeny homozygous for the desired genetic modification. Two pairs of mice were bred. A total of 39 pups were born and 9 of these pups were homozygous for the genetic modification.

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• 2005
  • 2005-10-19 EP EP05807480.8A patent/EP1802193B1/en not_active Expired - Lifetime
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  • 2005-10-19 ES ES05807480.8T patent/ES2463476T3/es not_active Expired - Lifetime
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  • 2005-10-19 CA CA2827654A patent/CA2827654C/en not_active Expired - Lifetime
  • 2005-10-19 WO PCT/US2005/037584 patent/WO2006044962A1/en not_active Ceased
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  • 2005-10-19 PT PT58074808T patent/PT1802193E/pt unknown
  • 2005-10-19 SI SI200531866T patent/SI1802193T1/sl unknown
  • 2005-10-19 AU AU2005295269A patent/AU2005295269B2/en not_active Expired
• 2007
  • 2007-09-27 US US11/904,558 patent/US7659442B2/en not_active Expired - Lifetime
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• 2014
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• 2016
  • 2016-07-11 US US15/207,388 patent/US9730434B2/en not_active Expired - Lifetime
• 2017
  • 2017-06-29 US US15/638,136 patent/US10039269B2/en not_active Expired - Lifetime
• 2018
  • 2018-04-26 CY CY20181100440T patent/CY1120496T1/el unknown

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